Publications

studies on tissue cholinesterase in experimental trichinosis in albino rat. 2 effects of treatment with mintezol and 5-fluorouracil-endoxan.	the changes in cholinesterase (che) activity was studied in different tissues of normal and 60 days t. spiralis infected albino rats following treatment with mintezol (5 mg/rat/day, for 5 days) and 5-fluorouracil-endoxan (500 mg/rat/day--100 mg/rat/day, for 5 days). in normal rats, administration of either mintezol or 5-fluorouracil-endoxan provoked a general decrease in the che activity of the various rat tissues. treatment of 60 days t. spiralis infected rats with mintezol increased markedly t ...	1993	8482867
studies on tissue cholinesterase in experimental trichinosis in albino rat. 1. effect of t. spiralis infection.	adult male albino rats were orally infected with trichinella spiralis (t. spiralis) larvae (400 larvae/rat). the cholinesterase (che) activity was then determined in the serum, brain, spinal cord, liver, stomach, intestine, heart, diaphragm and gastrocnemius muscle of the infected rats at different time intervals (15, 30, 45, 60 and 90 days) after infection. the enzyme activity was inhibited at all the time intervals in the brain, liver, heart and diaphragm while it increased progressively in th ...	1993	8482886
trichinella spiralis infected skeletal muscle cells arrest in g2/m and cease muscle gene expression.	infection by trichinella spiralis causes a variety of changes in skeletal muscle cells including the hypertrophy of nuclei and decreased expression of muscle specific proteins. potential cellular processes leading to these changes were investigated. in synchronized muscle infections, [3h]thymidine was incorporated into infected cell nuclei from 2-5 days post infection. labeled nuclei were stably integrated into the infected cell up to 60 days post infection and appear to originate from different ...	1993	8491772
human recombinant interleukin 1 beta suppresses acetylcholine release from rat myenteric plexus.	a marked suppression of acetylcholine (ach) release from myenteric nerves in the inflamed intestine of rats infected by trichinella spiralis has been shown. in this model, there is increased expression of interleukin 1 beta (il-1 beta) in the myenteric plexus. therefore, the ability of il-1 beta to alter ach release in longitudinal muscle-myenteric plexus (lmmp) preparations from noninfected rats was examined.	1993	8500722
biochemical, biological, and genetic characterization of a sylvatic isolate of trichinella.	biological, biochemical, and genetic relationships of trichinella isolates were assessed and compared from 3 species of illinois fur-bearing mammals. tongue muscle collected from 1987 through 1989 from 323 raccoons (procyon lotor), 9 red fox (vulpes fulva), and 1 coyote (canis latrans) were digested and trichinella muscle larval prevalences and mean intensities (larvae/g) determined. the prevalence and mean intensity of tongue muscle-stage larvae were 2.8% and 44.4% and 326 and 2 larvae/g for ra ...	1993	8501590
arbitrarily primed polymerase chain reaction of individual trichinella specimens.	recently, 5 sibling species and 3 other phenotypes were identified in the genus trichinella. single primers of arbitrary nucleotide sequence were used to produce random amplified polymorphic dna starting from decreasing amounts of trichinella spiralis and trichinella britovi dna. reproducible amplification products from 30 pg of dna were obtained using 1 of 6 examined primers. these fragments distinguish between 2 european trichinella species, t. spiralis, showing a 1,350-bp band, and t. britovi ...	1993	8501603
a morphological study of the effects of liposomized albendazole on the muscle phase of trichinella spiralis in mice.	the pathomorphological effects of daily and weekly doses of liposomized albendazole on trichinella spiralis larvae have been examined and compared during the course of muscle infections in mice. treatment with three and six daily doses of the drug given by intraperitoneal injection to mice resulted in marked pathological alterations to encapsulated larvae, mainly in the walls of the capsules. there was a 5% efficacy against t. spiralis in mice given three daily doses of albendazole, and 38% effi ...	1993	8509615
trichinosis with ventilatory failure and persistent myocarditis.	life-threatening infections with trichinella spiralis are rare in countries that have adopted laws requiring cooking of raw garbage fed to pigs. thus such infections may pose a diagnostic dilemma for clinicians unfamiliar with their presentation. we report a case of imported trichinosis in a mexican national who developed respiratory failure, myocarditis, and sinus arrest. the patient recovered uneventfully after the administration of benzimidazole and corticosteroid drugs, although a pacemaker ...	1993	8513055
trichinella spiralis: the effect of specific antibody on muscle larvae in the small intestines of weaned rats.	weaned rats were passively immunized with rat igg2c monoclonal antibodies previously shown to protect infant rats against challenge with trichinella spiralis. although the antibodies did not protect rats against infection, the course of larval establishment in the intestine was altered in a way that has not been described previously. specifically, larvae that invaded the epithelium were inhibited from migrating from intestinal tissue into saline during the standard recovery procedure. the inhibi ...	1993	8513877
the mucosal and systemic response to phosphorylcholine in mice infected with trichinella spiralis.	the presence of phosphorylcholine (pc) in trichinella was confirmed by elisa and western blot experiments with the pc-specific myeloma tepc-15. anti-pc antibody production was detected in elisa by cross-reaction with the pc-positive somatic polysaccharide of aspergillus and the synthetic conjugate phosphorylcholine-bovine serum albumin conjugate and by inhibition with phosphorylcholine chloride (pccl). the kinetics of the serum and mucosal anti-pc immunoglobulin response were determined followin ...	1993	8513878
treatment of experimentally induced trichinosis in dogs and cats.	studies were undertaken to determine the efficacy of milbemycin oxime against the enteric adult stages of trichinella spiralis and of albendazole against the muscle stage larvae in experimentally infected dogs and cats. specific-pathogen-free beagle pups (n = 6) and domestic shorthair kittens (n = 6) were inoculated with 7,500 first-stage larvae of trichinella spiralis. physical examination (including collection of blood and fecal samples) was performed weekly. during the first week after inocul ...	1993	8214900
dynamics of rat muscle mitochondria uncoupling in trichinellosis.	parallel oxygraphic measurements of respiratory control index (rci) and spectroscopic measurements of the activity of two mitochondrial enzymes from rat muscle, mtatpase and succinate dehydrogenase were performed. in trichinella spiralis infection, in comparison with trichinella pseudospiralis infection, a delayed stimulation of mtatpase in 3rd week p.i. was observed and the degree of stimulation was weaker. a second peak of mtatpase stimulation appeared at the beginning of the encystation phase ...	1993	8225761
human humoral immune responses against trichinella spiralis.	the analysis of trichinellosis patients' sera was performed by western blot against a crude extract of trichinella spiralis. eight distinctive bands from 38 to 104 kda were present in most of the patients' sera as a striking homogeneous response. some of these characteristic bands were found to be recognized by the serum of infected rodents and of an immunized rabbit in this work, and have also been reported to be recognized by monoclonal and polyclonal antibodies from t. spiralis infected or im ...	1993	8225769
vaccination against trichinella spiralis in mice using antigens from different isolates.	antigen preparations from three isolates of trichinella spiralis (sensu-stricto) have been tested for their ability to immunize mice against homologous and heterologous challenge infections. immunization was measured by accelerated expulsion of adult worms, reduction in female worm fecundity, stunting of growth, and level of antibody responses before and after challenge. antigens of two isolates, from london (l) and poland (p), immunized effectively against homologous challenge, adult worm recov ...	1993	8233594
superoxide dismutase of trichinella spiralis and trichinella pseudospiralis larvae.	the aim of this study was to compare the in vitro and in vivo activities of superoxide dismutase, an enzyme acting against free-radicals, in trichinella spiralis and t. pseudospiralis isolates. in vitro, the activity of the superoxide dismutase in t. pseudospiralis was determined to be about two times higher than in t. spiralis. only one band representing superoxide dismutase was found by electrophoretic analysis. in vivo, in muscles infected with t. spiralis, a strong histochemical reaction in ...	1993	8256096
characterization of novel fucosyl- and tyvelosyl-containing glycoconjugates from trichinella spiralis muscle stage larvae.	the monosaccharide composition of an affinity-purified family of antigenically-related trichinella spiralis larval glycoproteins was determined by gas chromatography/mass spectrometry. this group of 6 major glycoproteins, designated tsl-1, originates in the muscle stage (l1) larval stichosome. they are present on the l1 surface and in excretory/secretory products of l1 larvae, are stage-specific, and are highly immunodominant. the glycosyl composition of the tsl-1 antigens was remarkable in 2 re ...	1993	8259130
serosurvey of some major zoonotic infections in children and teenagers in bali, indonesia.	one hundred and ninety serum samples, mainly from children and teenagers, from northeast bali were tested for the presence of antibodies against five zoonotic agents: brucella abortus, coxiella burnetii, toxoplasma gondii, toxocara canis and trichinella spiralis. all children were negative for brucellosis and q fever. a high prevalence rate was found for toxocariasis (63.2%) and trichinosis (19.5%). antibody prevalence against t. gondii was found to be rather low (3.1%). to our knowledge, this i ...	1993	8266236
trichinosis: a prospective controlled study of patients ten years after acute infection.	the existence of chronic trichinosis as a disease entity is still a matter of debate. for 10 years after an outbreak of infection with trichinella spiralis, we conducted a prospective controlled study of the patients involved. at the termination of this investigation, we undertook clinical, biochemical, serological, immunologic, neuroradiological, radiological, and psychological studies of 128 originally infected persons and 16 controls. the categories of symptoms most often documented in person ...	1993	8268344
random amplified polymorphic dna technique for the identification of trichinella species.	the random amplified polymorphic dna (rapd) technique was successfully used to produce genetic fingerprints distinguishing between trichinella spiralis and trichinella britovi. the same patterns were obtained from purified and crude dna preparations of pooled and single muscle larvae. rapd fingerprinting was applied to muscle larvae preserved under different conditions and recovered from different hosts. larvae recovered from fresh and frozen meat and stored at -20 degrees c for a long time or u ...	1993	8278221
effects of levamisole on ethanol-induced suppression of lactational immune transfer in rats.	maternal ethanol consumption in rats has been shown to inhibit lactational transfer of immunity to trichinella spiralis (t. spiralis) from dams to their neonates. the purpose of this study was to determine if this depressed immune transfer could be altered by treating the dams with a known immunostimulatory drug during pregnancy and lactation. groups of female rats were fed ethanol-containing or were pair-fed isocaloric control liquid diets for 30 days, infected orally with 1,000 t. spiralis lar ...	1993	8279681
[comparison of numbers of active lymphocytes b in spleen of mice infected with trichinella pseudospiralis or trichinella spiralis].	the experiments were performed on balb/c mice (male, 20 g body weight). the mice were infected 23 days before the experiment by peroral application of about 100 trichinella larves. control group consisted of not infected mice. the number of lymphocytes b producing antibodies was determined according to cunningham and szenberg. five days before performance of the test, all mice were immunized by peritoneal application of sheep erythrocyte suspension. after that the mice were sacrificed, spleen wa ...	1993	8189819
[2 outbreaks of trichinelliasis in rostov province].	two epidemiologically unrelated outbreaks of trichinellosis were registered in rostov province in the november and december of 1984 with 16 patients in the salsk district and 20 patients in rostov-on-don. the course of the disease was characterized by 6-20% of severe and 31-45% moderate forms, respectively. the source of the infection was pork that had not been controlled for trichinella spiralis. late diagnosis and inadequate treatment in one case led to a fatal outcome. economic losses account ...	1993	8127264
in vitro tnf, il-6 and il-2 production by rat milk cells following trichinella spiralis infection.	trichinella spiralis-specific immunity can be transferred from immune mothers to suckling neonates via lactation, suggesting that milk from immune dams contains specific factors to t. spiralis. tnf and il-6 are important cytokines in inflammatory processes, and il-2 is essential in lymphocyte activation. using cell line bioassays, we examined the capacity of rat milk mononuclear cells from immune and non-immune control dams to produce these cytokines following in vitro stimulation with mitogens ...	1993	8164194
molecular variation in trichinella.	the taxonomic status of variants within the genus trichinella is problematical. some authors recognise no fewer than four species (trichinella spiralis, t. pseudospiralis, t. nativa and t. nelsoni), others regard t. nativa and t. nelsoni as strains of t. spiralis (t. spiralis var nativa or sylvatica), while others consider the genus to be monospecific, with a variety of more or less well defined isolates. much of the current evidence adduced to support these various positions is similar to that ...	1993	8100678
relationships between the anthelmintic activity of eight derivatives of benzimidazole carbamates against trichinella spiralis and their chemical structures.	efficacy of eight recently developed and used anthelmintics of the benzimidazole carbamates; mebendazole, flubendazole, oxfendazole, albendazole, oxibendazole, 790163 proflubendazole, 780118 "cyanide" benzimidazole and 780120 "selenium" benzimidazole was tested orally against the enteral immature larval and adult stages of trichinella spiralis in mice. six of these derivatives of methyl benzimidazole-2-carbamates have an aryl and two have an alkyl substituent at the 5'-position of the parent ben ...	1993	8051807
biochemical characterisation of trichinella spiralis and t. pseudospiralis stichocyte antigens.	one and two dimensional western blots of trichinella spiralis and trichinella pseudospiralis extracts were probed with a monoclonal antibody (7c2c5) which recognizes molecules found in the stichocytes. three major antigens were revealed in excreted:secreted (e/s) preparations at approx mr 45, 53 & 60 x 10(3). the mr 45 x 10(3) molecule was the most abundant secreted antigen, on two dimensional analysis it resolved into many isoforms with a pi range 4.5-6.4 in t. spiralis and into four isoforms p ...	1993	8298662
characteristics of t lymphocyte cell lines established from nih minipigs challenge inoculated with trichinella spiralis.	mhc restricted, antigen specific, cytokine dependent t-cell lines were established from peripheral blood mononuclear cells (pbmc) of nih minipigs that were challenge inoculated with trichinella spiralis. swine lymphocyte antigen (sla) inbred nih minipigs of the sla a/a (aa) haplotype can be divided into responder (r) and non-responder (nr) phenotypes on the basis of their ability to destroy encysted muscle larval (ml) forms of the parasite t. spiralis. when orally inoculated with 300 infectious ...	1993	7679239
the in vivo role of stem cell factor (c-kit ligand) on mastocytosis and host protective immunity to the intestinal nematode trichinella spiralis in mice.	the role of stem cell factor (scf) in the generation of intestinal mast cell hyperplasia and host protective immunity following helminth infection was investigated using the trichinella spiralis/mouse model. in vivo administration of a monoclonal antibody specific for the receptor for scf (c-kit) was found to completely prevent the generation of intestinal mastocytosis normally observed following t. spiralis infection. this was reflected by markedly reduced intestinal mast cell protease (imcp) l ...	1993	7679484
identification of potential antigenic targets for rapid expulsion of trichinella spiralis.	rat monoclonal antibodies, which have been shown to mediate rapid expulsion of trichinella spiralis in infant rats, were used in immunochemical analyses to identify the antigens targeted in protection. protective antibodies bound to gp45, and to a series of related antigens, in the excretory-secretory products of muscle stage larvae. non-protective antibodies did not bind gp45, but did bind the related species. the latter were shown to be present as aggregates (dimers and multimers) in excretory ...	1993	7681546
altered expression of sodium pump isoforms in the inflamed intestine of trichinella spiralis-infected rats.	we used polymerase chain reaction (pcr) and northern analysis to examine the effect of trichinella spiralis infection on mrna expression of sodium pump isoforms in rat intestine. 28s rna was coamplified as an internal standard, and the abundance of the isoformic mrna was expressed relative to 28s rna. as assessed by pcr and northern analysis, the alpha 1- and beta 1-subunits were expressed in the jejunum and ileum of noninfected control rats. however, in the jejunum the levels were suppressed af ...	1993	7687413
regional content of enteric substance p and vasoactive intestinal peptide during intestinal inflammation in the parasitized ferret.	our aim was to characterize and quantitate changes in two key neuropeptides, substance p (sp) and vasoactive intestinal peptide (vip), that are involved in governing neurally-mediated gastrointestinal (gi) reflex activity during enteric inflammation in the ferret. neuropeptide content was determined by radioimmunoassay of extracts of jejunal, ileal and colonic muscularis externa from uninfected ferrets and ferrets infected with enteric stages of the parasitic nematode, trichinella spiralis. incr ...	1993	7692345
inhibition of murine intestinal inflammation by anti-substance p antibody.	several neuropeptides have recently been shown to affect various aspects of the inflammatory process. among these, the neuropeptide substance p possesses a host of immune modifying actions, which include the enhancement of lymphocyte activity, macrophage function, and neutrophil chemotaxis. the role of substance p during inflamed states has, as yet, not been fully described. here, in t. spiralis-infected mice, we parallel increased levels of substance p both locally, (the gut) and peripherally ( ...	1993	7692913
[pulmonary echinococcosis, hepatic opisthorchiasis and generalized trichinelliasis combined with pregnancy].	a case is reported of a spontaneous abortion (25 weeks of gestation) in a nenets woman resulting in thrombohemorrhagic syndrome and profuse uterine bleeding. the abortion is attributed to drastic allergization by three parasites: echinococcus granulosus, opisthorchis felineus, trichinella spiralis.	1993	7980070
immune killing of newborn trichinella larvae by human leucocytes.	the capacity of human leucocytes from normal donors to kill the newborn larvae of the nematode trichinella spiralis in vitro, in the presence of serum from infected individuals, was studied using newborn larvae (nbl) less than 2 h of age or nbl that had been maintained in culture at 37 degrees c for 20 h. neutrophils and monocytes attached to newborn trichinella larvae and killed them, regardless of their age. when eosinophils were used, 20 h old nbl were killed whereas 2 h old nbl were not. com ...	1993	7877832
interleukin 1 beta-induced increase in substance p in rat myenteric plexus.	substance p (sp) is increased in the inflamed intestine of trichinella spiralis-infected rats, but the underlying mechanism is unknown. interleukin 1 beta (il-1 beta) messenger rna and protein is expressed in the longitudinal muscle-myenteric plexus (lm-mp) of this model. thus, the purpose of the study was to examine the ability of human recombinant il-1 beta (hril-1 beta) to increase sp in lm-mp preparations from the intestine of noninfected rats.	1993	7504644
an evaluation of the role of carbohydrate epitopes in immunity to trichinella spiralis.	a study is described in which the role of carbohydrate epitopes in the generation of protective immunity to trichinella spiralis was investigated. antigen preparations were treated with increasing molar concentrations of sodium periodate, which is known to degrade carbohydrate moieties by cleaving one side of the hexose ring, and a suitable protocol was established for the selective degradation of carbohydrates with the retention of protein integrity. using excretory/secretory (es) proteins, bot ...	1992	1279505
synthesis and characterization of rat interleukin-10 (il-10) cdna clones from the rna of cultured ox8- ox22- thoracic duct t cells.	a cdna of the complete coding region of rat il-10 was cloned and sequenced using rna isolated from a cultured population of thoracic duct t-lymphocytes obtained from trichinella spiralis infected animals. the ox8- ox22-t-helper cells were stimulated in vitro with concanavalin a for 24 hours prior to harvest. reverse transcription of cellular rna was primed with oligo-dt followed by amplification of il-10 specific cdna by polymerase chain reaction with synthetic oligo nucleotide primers chosen fr ...	1992	1280414
trichinella spiralis: specificity of es antigens from pre-encysted larvae.	excretory/secretory (es) antigens were obtained by culturing pre-encysted trichinella spiralis larvae which were recovered from muscles of experimentally infected mice 14-15 days postinfection. analyses of these antigens (pel es) with immunoblotting, sds-page and triple antibody elisa showed that they yielded a low sensitivity and specificity when tested with antisera against the common nematodes of chinese pigs. as compared to es antigens from encysted larvae, pel es also contained more low mol ...	1992	1281854
an outbreak of trichinosis in lebanon.	in 1982 an outbreak of trichinosis occurred in an area of south lebanon consisting of 4 villages, including 6440 persons. in 267 households, involving 2456 persons, typical clinical signs of trichinella spiralis infection could be recognized among household members. twenty-one of these households, including 193 persons, were randomly chosen for further studies. nearly half of the household members (46%) had clinical symptoms consistent with acute trichinosis. 12 of 16 tested persons had high ant ...	1992	1287937
immunodiagnosis of human trichinellosis using excretory-secretory (es) antigen.	infective first stage larvae of trichinella spiralis were recovered from muscles of laboratory infected mice by digesting the muscles with 1% hc1-1% pepsin and collecting the larvae by modified baerman's method. the larvae were cultivated in a serum-free medium for 18 h. the es antigen obtained from the culture medium was used in an enzyme-linked immunosorbent assay (elisa) for detecting igg antibodies to t. spiralis in serum samples collected from three groups of individuals. the individuals of ...	1992	1293197
[trichinellosis focus resulting from consumption of wild boar meat].	the focus of trichinellosis was presented comprising 28 patients and resulting from consumption of the wild boar meat. early confirmation of trichinellosis diagnosis in the first case (index case) and an accurate epidemiological analysis established that the patients became infected with trichinella spiralis strain originating from natural environment. a severe clinical course was disclosed in the index case, moderate course of trichinellosis in 11 patients, a mild course in 15 cases and an abor ...	1992	1296244
[establishment and preliminary application of competitive elisa using monoclonal antibody against trichinella spiralis].	monoclonal antibody competitive elisa was devised with a highly specific mcab (2g8) against t. spiralis. among 20 rabbits infected with t. spiralis experimentally. 35% (7/20) were positive on the 17th day post infection (pi); 100% positive on the 31st day pi. no cross reaction was found with 29 sera of rabbits infected with other kinds of helminth. the dynamic antibody titer of five infected rabbits sera were studied with this method. the change may be helpful to judge the state of infection. in ...	1992	1298715
[a method for acquiring newborn larvae of trichinella spiralis in vitro].	this paper reports a simple method for acquiring numerous newborn larvae of trichinella spiralis in vitro. adults of t. spiralis, collected from small intestine on rats which were infected with infective larvae separated from mice infected experimentally, were put into tissue culture bottle containing m199. then the bottles were incubated in co2 incubator (37 degrees c, 5% co2) for 15-20h. the newborn larvae were collected by passing through filtration, centrifugation and repeated wash. the resu ...	1992	1298727
[influence of cadmium on the course of intestinal and muscular phases in mice infected with trichinella spiralis].	at the first experiment 20 swiss male mice were infected with 100 larvae and 20 mice with 500 larvae t. spiralis per mouse. two days after infection (d.a.i.) mice orally received 1.5 mg cd (water solution cdcl2) each. 40 mice were infected only t. spiralis as control. at the second experiment muscle larvae used were isolated from mice (from the first experiment) which received cd. 20 mice were infected with 100 larvae and 20-with 500 larvae per mouse. two d.a.i. mice received 1.5 mg cd. mice fro ...	1992	1299056
activity of natural killer (nk) cells in the course of experimental trichinellosis in mice.	b6c3f1 mice were infected with 200 or 500 larvae of trichinella spiralis per mouse and pulmonary nk cell-mediated clearance of semisyngeneic tumour cells was determined in vivo on days 10, 20, 30, and 60 after the infection. cytotoxic activity of nk cells in the lungs was substantially elevated on days 20 and 30 after challenge with both "doses" of the parasite. at the same time large granular lymphocytes (lgls) as well as cells expressing surface asialo-gm1 molecules were isolated in elevated n ...	1992	1299057
activity of specific igg, igm and ige antibodies in human trichinellosis.	the activity of igg, igm and ige antibodies to somatic antigen of trichinella spiralis in the sera of patients with trichinellosis at various intervals after infection was examined by means of elisa. mathematical analysis of the dose-response curves was used. elevated level of igg and igm antibodies of relatively high avidity and of rather low ige avidity was documented. amount or avidity of igg antibodies was found to be most useful for the diagnosis of trichinellosis (85% positive results in p ...	1992	1299058
[influence of nalcrom (sodium cromoglycate) on the course of the intestinal phase of trichinellosis in mice].	the influence of nalcrom (sodium cromoglycate) on the course of the intestinal phase of trichinellosis in mice was investigated. the animals infected with 200 trichinella spiralis larvae were treated with nalcrom between 7-20 or 3-20 days after infection (d.a.i.). the drug was administered in two doses: 0.6 or 1.7 mg/mouse/day. in the all groups of animals received nalcrom higher number of mast cells and eosinophils than in the control groups was observed. these results are the opposite of those ...	1992	1299059
efficacy of albendazole against trichinella pseudospiralis and trichinella spiralis in mice.	albendazole (smith kline, beecham) in a dose of 20 mg/kg/day was given to b6c3f1 mice exposed to 300 larvae of t. pseudospiralis or t. spiralis. the drug was introduced on days 2, 3 and 4; or 4, 5 and 6; or 18, 19 and 20 after infection. it limited the intensity of intestinal and muscle phases of trichinellosis. given at the stage of maturation of adult forms and production of new-born larvae, the drug caused almost total elimination of both species from the intestines of mice and a decrease in ...	1992	1299060
trichinellosis in the ussr (1983-1987), tendency to spreading.		1992	1299061
[the role of the nucleus in the mechanism of transformation of muscle after infection by trichinella spiralis larvae. iii. morphometric analysis of the functional transformation of the muscle cell nucleus after infection].	morphometric investigations (using the convergence analysis method) of geometric parameters of nuclei and nucleoli of transformed muscle cells were carried in mice 15 days, 30 days and 6 month after infection with t. spiralis larvae. the analysis showed the largest increase of investigated the parameters of 15th day after the infection. the results of morphometric analysis are in agreement with the morphologic, ultrastructural and histochemical observations (published in i and ii part) as for as ...	1992	1299069
[current prevalence of trichinosis in santiago, chile (1992). a study on 500 corpses].	in august-december 1992, 500 diaphragm muscle samples taken at random from people who were autopsied at the santiago medico-legal service, were submitted to phototrichinoscopy to search for encysted larvae of trichinella spiralis. for each sample seven small pieces were examined. three hundred and eighty nine (77.8%) cadavers corresponded to males and one hundred and eleven (22.2%) to females. ages ranged from 1 to 90 years. ten (2.0%) of the individuals examined resulted positive, the higher fr ...	1992	1307006
impaired acetylcholine release in the inflamed rat intestine is t cell independent.	we investigated mechanisms underlying the suppression of [3h]acetylcholine ([3h]ach) release from myenteric plexus-longitudinal muscle preparations of rats infected 6 days previously with trichinella spiralis. there was a 73% suppression of kcl-evoked release of [3h]ach in the jejunum, and a 76% suppression was observed in the worm-free ileum, indicating that the local presence of the parasite in the lumen is not prerequisite for the suppression of ach release from the myenteric plexus. treatmen ...	1992	1325127
the significance of the "lung phase" in host-helminth relations.	many helminth infections have a "lung phase" whose significance considering the host-parasite relations has not yet been clarified. in some nematode infections larvae must pass through the lungs, in others adult worms live in the respiratory tract, and some others cause tropical pulmonary eosinophilia. some examples of these infections (nippostrongylus brasiliensis, schistosoma mansoni, trichinella spiralis and toxocara canis) are considered to clarify whether this phase of infection is a "pro-h ...	1992	1339975
[susceptibility and the immune response to trichinella spiralis infection].	the aim of this study was to assess a possible association between parameters of immune response and susceptibility/resistance to t. spiralis infection. the immune response to t. spiralis was monitored in two inbred strains of mice (balb/c and c57bl/6), as well as in the most important natural host--swine, by analysing the changes in main lymphocyte populations, the appearance of specific antibodies and worm burden recovery. in the murine model, the lower level of muscle larvae worm burden recov ...	1992	1340488
recognition of trichinella spiralis muscle larvae antigens by sera from human infected with this parasite and its potential use in diagnosis.	human antibody response to total soluble extract of trichinella spiralis muscle larvae (tse) was analyzed by western blot. the most frequently recognized antigens had molecular weights of 96, 67, 63, 60, 55 and 47 kda. an antigenic fraction containing two peptides with m.w. of 43, 47 kda from the parasite (p43, 47 ts l1) was isolated by elution from polyacrylamide gel slabs. it was used as antigen in an elisa test and compared to that of tse. serum samples from 51 symptomatic trichinellosis pati ...	1992	1345318
influence of resistant and susceptible genotype, il-1, and lymphoid organ on trichinella spiralis-induced cytokine secretion.	the relative importance of cell-mediated inflammatory responses and antibody-mediated responses in controlling parasitic helminth infection is debated. to study the relationship between these responses and resistance or susceptibility to primary trichinella spiralis infection, we infected resistant akr mice and susceptible b10.br mice and analyzed the lymphokines il-2, ifn-gamma, and il-5 produced by their t cells as a function of time and lymphoid organ. il-2-secretors occurred maximally betwee ...	1992	1353100
characterization of cholinesterases from the parasitic nematode trichinella spiralis.	1. trichinella cholinesterases occur in multiple molecular forms which differ in size, kinetics, activity with butyrylthiocholine, and effects of inhibitors. 2. the 5.3 and 13s forms identified in trichinella extracts are also found in c. elegans and other nematodes but the 7s form which occurs in other nematodes was absent from trichinella detergent extracts. differences in kinetic and inhibition properties among nematode species were also evident. 3. the level of cholinesterases in excretory/s ...	1992	1360365
[soluble metabolic antigens of trichinella spiralis: their isolation and characteristics].	cultivation of trichinella muscular larvae, purified by centrifugation in 20 ... 50% saccharose density gradient, in protein--free nutrient media at a dosage of 3.5-.10(3) lar./ml in the presence of insulin has made it possible to obtain a soluble antigen of trichinella. it has been shown by means of electrophoresis in polyacrylamide gel that the soluble (secretory-excretory) antigen has three protein fractions while the somatic trichinella antigen has 18 fractions. it has been shown that the so ...	1992	1364527
specificity of affinity-purified trichinella spiralis antigens.	an affinity-purified fraction (apf) was obtained by passing crude somatic antigens of trichinella spiralis muscle larvae through an affi-gel 10 column coupled with anti-trichuris suis igg. the fraction contained seven antigens with molecular weights ranging from 28 to 55 kda. when tested with antiserum against other common nematodes of pigs from china, the apf was found to be markedly more specific than s3 antigens (prepared by a combination of cell fractionation and differential centrifugation ...	1992	1373256
increased levels of substance p in the myenteric plexus of trichinella-infected rats.	changes in immunoreactive substance p concentrations were investigated in longitudinal muscle-myenteric plexus preparations from the inflamed jejunum of trichinella spiralis-infected rats. the substance p concentration increased within 2 days of infection and increased fivefold by day 6; in contrast, there was no significant increase in substance p in the noninflamed ileum. in vitro exposure of preparations from infected rats to scorpion venom reduced substance p levels by 88%. in addition, no i ...	1992	1375178
analysis of a 43-kda glycoprotein from the intracellular parasitic nematode trichinella spiralis.	the l1 larvae of the parasitic nematode trichinella spiralis invade skeletal muscle and initiate a process that has been interpreted to represent skeletal muscle dedifferentiation. in this process, the infected region of the muscle cell is converted into a unique structure, called the nurse cell. the nematode t. spiralis can survive for tens of years within the cytoplasm of the nurse cell and secretes proteins into the cytoplasm that are believed to play a role in mediating the nurse cell format ...	1992	1382055
[chemistry and immunochemistry analysis of the soluble antigens of newborn larvae of trichinella spiralis].	the soluble antigens of newborn larvae of trichinella spiralis were characterized in terms of molecular weight of protein, glycoprotein and lipoprotein contents, and immunochemistry. after the antigens were separated with sds-page and then followed by ultrasensitive silver staining, at least 40 bands of proteins were noted. correspondingly, 28 bands of glycoproteins and 9 bands of lipoproteins were revealed as the similar gels were stained by the hypersensitive periodic acid silver and nile's bl ...	1992	1383119
[changes in t lymphocytes and their subpopulations and igg in peripheral blood from mice infected with trichinella spiralis].	acid alpha-naphthyl esterase (anae), a cytoplasmic marker, was used to identify the t lymphocytes and their subpopulations in peripheral blood of mice infected with trichinella spiralis, and dot-elisa was used to identify the serum igg antibody of the infected mice. the results showed that t lymphocytes increased on the d3 after infection, reaching the peak on d14, and then remained in number greater than normal up to d77. the spotted granular anae positive cells (help t cells, th) decreased and ...	1992	1394900
[analysis of newborn larva of trichinella spiralis by immunoblot].	newborn larva (nbl) antigens of trichinella spiralis were analysed by immunoblot, and were compared with the adult and muscle larva antigens. the sds-page patterns of nbl somatic constituents consisted of about 40 polypeptide bands, which were obviously different from those of adult and muscle larva. immunoblot analysis indicated that immunization with nbl could induce a stage specific immune response. the molecular weight of specific nbl antigens were 129, 120, 89, 87, 79, 74, 72, 64, 58, 43, 4 ...	1992	1394901
boosted mucosal immune responsiveness in the rat intestine by actively transported hexose.	anaphylaxis-mediated intestinal fluid secretion was measured in trichinella spiralis- or ovalbumin-immunized rats challenged intraduodenally with t. spiralis somatic antigen (1 mg protein/0.5 ml saline) or ovalbumin (1 mg/0.5 ml saline), respectively. intestinal fluid volume was measured 30 minutes after challenge as an index of net secretion. challenge with the antigenic bolus containing 40 mmol/l d-glucose induced twice the fluid secretion as that induced by either antigen alone. l-glucose was ...	1992	1397873
minimizing elisa background in the diagnosis of swine trichinellosis.	after confirming that long-term serum storage (frozen at -20 c for greater than 3 mo) causes optical density to drift upward, several modifications of an enzyme-linked immunosorbent assay (elisa) protocol were evaluated to identify a protocol that would reduce background in porcine sera tested for trichinellosis. modifications evaluated included blocking the antigen-coated elisa plate with sample diluent containing 10% bovine serum albumin (bsa) or 10% nonfat milk powder (bovine lacto transfer o ...	1992	1403424
detection of circulating antigens in human trichinellosis.	a 'sandwich' enzyme-linked immunosorbent assay was established to detect circulating antigens of trichinella spiralis in human sera, its sensitivity and specificity was evaluated using 4 antigens (trichinella spiralis, trichuris trichiura, dirofilaria immitis and ascaris suum), and it was found to be sensitive and specific for t. spiralis antigen. samples of 347 individuals with suspected trichinellosis, who had eaten incompletely cooked bear meat containing larvae of t. spiralis, were examined. ...	1992	1412659
immunogenetic and evolutionary influences on the host-parasite relationship.	immunogenetic aspects of the host-parasite relationship between the laboratory mouse and the parasitic nematode trichinella spiralis are reviewed. variation in the capacity of hosts to express an effective protective immunity is associated primarily with variation in inflammatory responsiveness. analysis of the contributions of t lymphocytes, cytokines, and myeloid precursor populations to variation in one particular inflammatory component, eosinophilia, is described. variation in parasite immun ...	1992	1426489
immunization against geographical isolates of trichinella spiralis in mice.	partially purified antigen preparations from six isolates of trichinella spiralis were used to immunize mice. immunogenicity of the antigens was assessed in terms of antibody and lymphocyte responses and ability to stimulate protective immunity against challenge. isolate antigens showed considerable cross-reactivity, and all elicited protective responses. two major patterns of immunizing ability could be distinguished: (a) isolates that immunized well against heterologous challenge and elicited ...	1992	1428511
depletion of eosinophils by anti-il-5 monoclonal antibody treatment of mice infected with trichinella spiralis does not alter parasite burden or immunologic resistance to reinfection.	mechanisms of parasite killing by eosinophils are widely studied and are often implicated in mediating resistance to parasitic infection, especially in conjunction with specific antibodies. evidence for the eosinophil as an anti-parasite killer cell in vivo is limited and may not justify the belief that eosinophils engage and/or kill infective helminths. we reexamined this question in a mouse model of trichinosis in which antisera to eosinophils were previously used to show the requirement for e ...	1992	1431133
cyclosporin a in experimental trichinosis scanning electron microscopic study.	the surface morphology of adult and larvae of trichinella spiralis after cyclosporin a treatment was studied by scanning electron microscopy (sem) of fixed, dried and metal coated specimens. the earliest topographic changes revealed in adults after the drug treatment were in form of disorganization of the cuticle especially between the hypodermal pores which appeared somewhat thickened and irregular. other changes were in form of bullae formation covering the whole width of the adult surface. ot ...	1992	1431295
[a morphometric study of the bone marrow of mice infected with trichinella spiralis and t. pseudospiralis].	the study of cytograms of the marrow of mice with experimental t. spiralis and t. pseudospiralis invasion of 1 to 60 days revealed the marked activation of granulocytes and lymphoid cell proliferation with the much lower response of erythroid cells. the maximal response occurred at 21-35 days of invasion with subsequent diminution. in mice experimentally invaded with t. pseudospiralis the response of the marrow was less pronounced, but the period of active cell proliferation was prolonged.	1992	1435529
[the morphological changes in the organs of the lymphoid system in the experimental infection of mice with trichinella spiralis].	the dynamics of morphological changes in central and peripheral organs of the lymphoid system of mice experimentally invaded with trichinella spiralis was studied. the immune response of the host was shown to have two phases including two peaks and the suppression period. the activation of the lymphoid organs was observed in earlier intestinal and muscular phases, and the suppression was noted in migration phase of invasion.	1992	1435560
[the effect of specific therapy on the bone marrow reaction in experimental trichinelliasis].		1992	1435574
occurrence and characteristics of hypodense eosinophils in rats infected with trichinella spiralis.	hypodense eosinophils are observed in peripheral blood and tissue from patients with eosinophilia due to helminthic infections. in this study, the variation in eosinophil density was examined in rats during trichinella spiralis infection. hypodense eosinophils were observed in the peripheral blood in association with trichinella infection. in peritoneal fluid, which was representative of tissue fluid, a majority of eosinophils were hypodense regardless of the infection. during the course of tiss ...	1992	1437239
genetic influences upon eosinophilia and resistance in mice infected with trichinella spiralis.	genetic influences upon host variation in eosinophilia and resistance to helminth infection, and the relationship between these parameters, were investigated in 7 inbred and 1 hybrid strains of mice infected with trichinella spiralis. clear strain-dependent variations were observed in the maximum peripheral blood, bone marrow and spleen eosinophilia attained in infected animals. swr, nih and sjl strains of mice all gave high responses to infection; four congenic strains sharing the b10 backgroun ...	1992	1437268
variation in responsiveness to trichinella spiralis infection in inbred rat strains.	an analysis of interstrain variation between 12 inbred and 4 congenic rat strains in the expression of immunity against trichinella spiralis is reported. all rat strains expressed strong rapid expulsion which resulted in the elimination of 88-98% of a challenge infection of muscle larvae. in contrast, substantial interstrain variation in the rate of adult worm expulsion in the primary infection was evident. by day 10 after infection, buf and yo strains had less than 50 worms left in the intestin ...	1992	1437269
the effects of maternal ethanol consumption on lactational transfer of immunity to trichinella spiralis in rats.	transient immunity to the intestinal parasite trichinella spiralis can be transferred from the mother to the neonate during lactation. the goal of this study was to determine whether maternal ingestion of ethanol during pregnancy and lactation inhibited expression of anti- t. spiralis immunity in nursing pups. groups of female rats were infected with 1000 t. spiralis l1 larva, mated, and fed either ethanol-containing or isocaloric liquid diets and maintained on diets through pregnancy and lactat ...	1992	1443426
distribution of lymphocyte subsets in rat milk from normal and trichinella spiralis-infected rats.	we have shown that t. spiralis-specific t lymphocytes can mediate maternal-to-neonatal immunity during lactation. this study addresses the change of lymphocyte populations in rat milk during normal and disease conditions. two color flow cytometric analysis was performed for milk lymphocytes. t cells (ox19+) made up 45% of rat milk lymphocyte population. t helper cells (th) composed 35% of total t cells while t cytotoxic/suppressor (tcs) cells constituted 34%, giving a th/tcs ratio of 1.03. the c ...	1992	1453391
isolation, characterization, and transcription of the gene encoding mouse mast cell protease 7.	a gene that encodes mouse mast cell protease (mmcp) 7 (also known as mouse mast cell tryptase 2) was isolated by genomic cloning with a cdna that encodes mmcp-6, a tryptase in serosal mast cells. cdnas encoding mmcp-7 were isolated from a bone-marrow-derived mast cell cdna library. the mmcp-7 gene spans 2.3 kilobases and contains five exons rather than six, as found in the mmcp-6 and human mast cell tryptase i genes. comparison of the 5' end of the transcript with the genomic sequence indicated ...	1992	1454796
trichinosis from wild boar meat in gojjam, north-west ethiopia.	an outbreak of trichinosis associated with ingestion of meat from a wild boar in gojjam administrative region, ethiopia, is reported. of the 30 soldiers, 20 who ate the meat raw, became ill and 5 of them were admitted to the armed forces general hospital (afgh). even though they presented with a typical history and clinical features the disease was not even suspected at the peripheral hospital. the diagnosis was confirmed, at the afgh, by deltoid muscle biopsy in all the 5 cases. trichinosis bei ...	1992	1455537
[the helminth fauna of the red fox (vulpes vulpes linne, 1758) in nordhessen and ostwestfalen. 2. nematodes].	between november 1989 and june 1990 a total number of 397 foxes were examined for the presence of nematodes in the stomach and the small intestine and 403 foxes for the presence of trichinella spiralis larvae. the animals came from the districts of kassel, arnsberg and detmold. in 32.7% of the foxes infections with toxocara canis were found, in 11.1% toxascaris leonina, in 3.8% ancylostoma caninum, in 3.5% uncinaria stenocephala and in 2.3% capillaria species. in most cases the number of nematod ...	1992	1459030
abnormal thymus development and impaired function of the immune system in rats after prenatal exposure to aciclovir.	aciclovir (synonym: acyclovir) causes abnormal thymus development in rats. after treatment on day 10 of gestation a weight reduction of the organ is obvious in 21-day-old fetuses which persists postnatally. adult male rats exposed in utero to one or three injections of 100 mg aciclovir/kg body wt given to the dam on day 10 of pregnancy showed a reduction of the thymus weight to 333 +/- 158 mg and 276 +/- 61 mg (control: 428 +/- 92 mg; n = 10). corresponding alterations were detectable in female ...	1992	1463390
immunosuppression of in vivo and in vitro lymphocyte responses in swine induced by trichinella spiralis or excretory-secretory antigens of the parasite.	the in vivo and in vitro effects of trichinella spiralis excretory-secretory (es) antigens on porcine peripheral blood lymphocyte (pbl) responses induced with mitogens (phytohemagglutinin, pha; concanavalin a, con a; pokeweed mitogen, pwm) or unrelated antigen (protein a) were studied to determine whether es antigens depress lymphocyte responses in experimental swine trichinosis, and/or if this response was manifested after lymphocytes from infected pigs had been pretreated with es antigens. add ...	1992	1466133
persistence of igg, igm, and ige antibodies in human trichinosis.	an enzyme-linked immunosorbent assay (elisa) was used to detect serum igg, igm, and ige antibodies against trichinella spirals s. str. following an outbreak of human trichinosis in 1989 in chiang rai province, thailand, for two years and seven months in order to observe diagnostic sensitivity of elisa as well as the persistence of antibodies. it was found that the sensitivity of igg-elisa, as determined from biopsy-positive cases, reached 100% on the 50th day of infection. for igm-elisa, peak se ...	1992	1470835
significance of diaphragm sampling for determining larvicidal effect of flubendazole and mebendazole on trichinella spiralis in mice.		1992	1479783
proteolytic enzymes from trichinella spiralis larvae.	trichinella spiralis larvae infect their hosts by the penetration of small intestine enterocytes. the exact mechanism of penetration is unknown, but the presence of proteolytic enzymes is suspected. in this study, whole worm extracts and excretory-secretory (es) components were obtained and their proteolytic enzymes examined. enzymes from worm extracts were capable of hydrolysing azocoll, a general protease substrate in a wide range of ph (2-8), with maximal activity at ph 5. trichinella spirali ...	1992	1485414
allozymic and biological characters of trichinella pseudospiralis isolates from free-ranging animals.	to evaluate biological and biochemical variability in nonencapsulated trichinella isolates, biological and allozymic studies were conducted on isolates of trichinella collected from a raptoral bird (aquila rapax) and a fox (vulpes corsac) in kazakhstan and from a dasyurid marsupial (dasyurus maculatus) on the island of tasmania, australia. allozyme profiles of bird and marsupial isolates showed close similarity with the type isolate of trichinella pseudospiralis. the avian and fox isolates succe ...	1992	1491304
schistosome antigen gp50 is responsible for serological cross-reactivity with trichinella spiralis.	the 50-kda component (gp50) present in schistosoma mansoni eggs and secretions of the various life stages of the parasite was recognized by experimentally infected mice and by humans with s. mansoni, schistosoma haematobium, and schistosoma japonicum infection. all sera reacting with crude s. mansoni-soluble egg antigens (sea) also reacted strongly with gp50 in enzyme-linked immunosorbent assay. no reactivity against gp50 was seen with sera from individuals without schistosomiasis, with the exce ...	1992	1491314
prevalence of antibodies to toxoplasma gondii and trichinella spiralis in 509 pigs from 31 farms in oahu, hawaii.	serum samples from 509 pigs from 31 farms in oahu, hawaii were examined for antibodies to toxoplasma gondii using the agglutination test in dilutions of 1:25, 1:50, and 1:500 and for trichinella spiralis using the enzyme-linked immunosorbent assay. toxoplasma gondii antibodies were found in 48.5% of pigs. antibody titers were: 5.1% positive at 1:25 dilution, 28.6% positive at 1:50 dilution and 14.7% positive at 1:500 dilution. the prevalence of toxoplasma gondii antibodies in garbage-fed pigs (6 ...	1992	1496803
a trichinosis outbreak among southeast asian refugees.	the number of cases of trichinosis reported to centers for disease control has declined steadily, with an average of only 44 cases per year from 1984 through 1988. this decline was almost entirely due to a reduction in cases acquired from ingestion of fresh commercial pork. however, from july 21 through september 3, 1990, 90 (72%) of 125 southeast asian refugees from six states and canada developed trichinosis after attending or eating pork sausage taken from a wedding held in des moines, iowa, ...	1992	1510086
prevalence of trichinella spiralis in black bears (ursus americanus) from newfoundland and labrador, canada.	tongue and diaphragm samples from 158 black bears (ursus americanus) from newfoundland and labrador were examined for trichinella spiralis. no larvae were detected in samples from the island of newfoundland but one animal from the labrador samples was infected. the results of this and other studies suggest a lack of involvement of the black bear in a sylvatic cycle of t. spiralis in eastern canada.	1992	1512886
il-10 induces transcription of the gene for mouse mast cell protease-1, a serine protease preferentially expressed in mucosal mast cells of trichinella spiralis-infected mice.	mouse bone marrow-derived mast cells (bmmc) obtained by culturing progenitor cells with ril-3 express mouse mast cell protease (mmcp)-5 mrna but not mmcp-1 mrna or mmcp-4 mrna. in terms of mast cell differentiation, these transcripts encode one early-expressed and two late-expressed chymases, respectively. cdna and crna probes were used in rnase protection assays and rna blot analyses to study the expression of these three homologous protease genes in cultured mast cells and in helminth-infected ...	1992	1517575
[a family outbreak of trichinosis acquired in israel].	trichinosis is a parasitic disease transmitted to man by ingestion of contaminated raw meat, usually pork, containing cysts of trichinella spiralis. formerly appearing as serious epidemics, it has now become uncommon due to public health control measures. since the jewish and moslem religions forbid consumption of pork, the disease is even rarer in israel. the literature indicates that all the cases of trichinosis hospitalized in israel were contaminated abroad. we describe an israeli family in ...	1992	1526560
distribution of mast cells in intestinal muscle of nematode-sensitized rats.	we examined the distribution and functional integrity of mast cells in intestinal longitudinal muscle in rats sensitized by two previous infections with trichinella spiralis. a segment of jejunum was excluded from the gut before infection, and the remainder of the gut was anastomosed. few mast cells were seen in muscle of noninfected control rats except in the region of the jejunal anastomosis. in rats sensitized by t. spiralis infection, mast cells were increased in number in the jejunum and th ...	1992	1550236
identification of diagnostic antigens from trichinella spiralis.	the western blotting technique was used to determine the antigens of trichinella spiralis muscle larvae that were recognized by antibodies in sera from humans and pigs displaying t. spiralis infections. this resulted in the identification of several antigens that were recognized by all sera. some of these antigens, notably those that were recognized during the early stage of infection, cross-reacted with antibodies to other parasites. this cross-reactivity was caused by the presence of phosphory ...	1992	1557322
susceptibility of adult heligmosomoides polygyrus to intestinal inflammatory responses induced by heterologous infection.	adult h. polygyrus are capable of surviving for many months after primary exposure of mice to infective larvae, raising the possibility that worms of this species have inherent resistance to intestinal immune responses. accordingly experiments were carried out to determine whether h. polygyrus are resistant to the inflammatory changes elicited during the acute phase of the intestinal response to trichinella spiralis. adult worms were expelled from mice when their presence coincided with the most ...	1992	1563923
experimental reorganization of host muscle cells by excretory/secretory products of infective trichinella spiralis larvae.		1992	1566315
t lymphocyte dependent enteropathy in murine trichinella spiralis infection.	mice infected with trichinella spiralis developed significant enteropathy, comprising villus atrophy, crypt hyperplasia, goblet cell hyperplasia and a decrease in intra-epithelial lymphocyte numbers by 10 days post-infection, when most of the parasites had been expelled from the gut. however, worm expulsion was prevented by treatment with cyclosporin a and, despite a continued parasite burden, cyclosporin a treated animals had no villus atrophy or changes in inflammatory cell numbers. these resu ...	1992	1570173
rapid expulsion of trichinella spiralis in adult rats mediated by monoclonal antibodies of distinct igg isotypes.	the role of igg in rapid expulsion of trichinella spiralis in adult rats was analysed. in this experimental model, rats were first infected with an unrelated nematode heligmosomoides polygyrus, then 5-14 days later, immune serum, its fractions, or igg monoclonal antibody (mab) was transferred. rats were challenged with t. spiralis muscle larvae 24 hr after antibody transfer and intestinal worms counted at various times, up to 24 hr, after challenge. provided rats were exposed to h. polygyrus fir ...	1992	1572697