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interleukin-22-induced antimicrobial phospholipase a2 group iia mediates protective innate immunity of nonhematopoietic cells against listeria monocytogenes.listeria monocytogenes is a bacterial pathogen which establishes intracellular parasitism in various cells, including macrophages and nonhematopoietic cells, such as hepatocytes. it has been reported that several proinflammatory cytokines have pivotal roles in innate protection against l. monocytogenes infection. we found that a proinflammatory cytokine, interleukin 22 (il-22), was expressed by cd3(+) cd4(+) t cells at an early stage of l. monocytogenes infection in mice. to assess the influence ...201526644377
choice of reference-guided sequence assembler and snp caller for analysis of listeria monocytogenes short-read sequence data greatly influences rates of error.the influences that different programs and conditions have on error rates of single-nucleotide polymorphism (snp) analyses are poorly understood. using illumina short-read sequence data generated from listeria monocytogenes strain hpb5622, we assessed the performance of four snp callers (bcftools, freebayes, unifiedgenotyper, varscan) under a variety of conditions, including: (1) a range of sequencing coverages; (2) use of four popular reference-guided assemblers (burrows-wheeler aligner, novoal ...201526643440
biosynthesis of paenibacillin, a lantibiotic with n-terminal acetylation, by paenibacillus polymyxa.paenibacillin, a recently-discovered lantibiotic from paenibacillus polymyxa osy-df, showed potency against listeria monocytogenes, methicillin-resistant staphylococcus aureus and other gram-positive bacteria. the chemical structure of paenibacillin has been determined previously. this study was initiated to investigate the biosynthesis of paenibacillin, and to reveal unique features in its biosynthetic pathway. paenibacillin structural gene (paea) was identified by polymerase chain reaction (pc ...201526640048
[a clinical analysis of 16 patients with maternal listeriosis].to summarize the clinical characteristics and outcome of maternal listeriosis so as to improve the understanding of disease and the level of diagnosis and treatment.201526674793
rhombencephalitis due to listeria monocytogenes: a clinicopathologic study of a case.central nervous system infections caused by listeria monocytogenes usually manifest in the form of meningitis or meningoencephalitis, and are more common among immunosuppressed patients. brainstem encephalitis (rhombencephalitis) is less common and fatal if not recognized and treated early.201526671740
listeriolysin o, but not murine e-cadherin, is involved in invasion of listeria monocytogenes into murine liver parenchymal cells.human e-cadherin and listeriolysin o (llo) are involved in invasion of listeria monocytogenes into human liver parenchymal cells (lpc). yet, it remains to be determined whether murine e-cadherin and llo participate in invasion of l. monocytogenes into murine lpc. in the present study, involvement of murine e-cadherin and llo in invasion of l. monocytogenes into murine lpc was investigated. murine e-cadherin was expressed on murine lpc, but the expression became undetectable by insertion of trans ...201526668665
listeriosis phytotherapy: a review study on the effectiveness of iranian medicinal plants in treatment of listeriosis.listeria monocytogenes can be found in many processed foods, raw milk, dairy products, meat and meat products such as sausages, beef and fish products, seafoods, eggs, fruits, and vegetables such as radish and cabbage. this article is a review study on the iranian medicinal plants applied for treatment of listeriosis. information of this review article was obtained by searching various key words such as listeria monocytogenes, medicinal plants, plant extracts and essential oils among scientific ...201526682768
rapid detection of listeria by bacteriophage amplification and sers-lateral flow immunochromatography.a rapid listeria detection method was developed utilizing a511 bacteriophage amplification combined with surface-enhanced raman spectroscopy (sers) and lateral flow immunochromatography (lfi). anti-a511 antibodies were covalently linked to sers nanoparticles and printed onto nitrocellulose membranes. antibody-conjugated sers nanoparticles were used as quantifiable reporters. in the presence of a511, phage-sers nanoparticle complexes were arrested and concentrated as a visible test line, which wa ...201526694448
ongoing outbreak of invasive listeriosis, germany, 2012 to 2015.listeriosis patient isolates in germany have shown a new identical pulsed-field gel electrophoresis (pfge) pattern since 2012 (n = 66). almost all isolates (listeria monocytogenes serotype 1/2a) belonged to cases living in southern germany, indicating an outbreak with a so far unknown source. case numbers in 2015 are high (n = 28). no outbreak cases outside germany have been reported. next generation sequencing revealed the unique cluster type ct1248 and confirmed the outbreak. investigations in ...201526691727
[quantitative detection on contamination of listeria monocytogenes isolated from retail ready-to-eat meats in beijing].to survey the contamination of l. monocytogenes isolated from retail ready-to-eat meats in beijing.201526738383
functional heterogeneity in cd4(+) t cell responses against a bacterial pathogen.to investigate how cd4(+) t cells function against a bacterial pathogen, we generated a listeria monocytogenes-specific cd4(+) t cell model. in this system, two tcrtg mouse lines, llo56 and llo118, recognize the same immunodominant epitope (llo190-205) of l. monocytogenes and have identical in vitro responses. however, in vivo llo56 and llo118 display vastly different responses during both primary and secondary infection. llo118 dominates in the primary response and in providing cd8 t cell help. ...201526697015
occurrence and antibiotic susceptibility of listeria species in turkey meats.the aims of this study were to investigate the occurrence of listeria species in turkey meats and to check the antimicrobial susceptibility of the isolated strains. hundred and fifteen raw turkey meat samples were randomly collected from the supermarkets, butchers and restaurants. strain isolation and identification were made according to the iso11290-1 method. antimicrobial susceptibility was determined by the standard disc diffusion method. a total of 47 listeria spp. were isolated from 115 (4 ...201526761896
survey of yogurt powder storage in ambient export countries a safety evaluation standard compliance and comparative analysis.yogurt powder is fermented milk processed in the form of dry yogurt, and has advantages such as stability, storability, convenience, and portability. china and vietnam are important export target countries because of the increased demand for dairy products. therefore, we surveyed dairy product standardization in order to establish an export strategy. lactic acid bacteria counts are unregulated in korea and vietnam. in china, lactic acid bacteria counts are regulated at 1×10(6) colony-forming uni ...201526761812
growth modelling of listeria monocytogenes in korean pork bulgogi stored at isothermal conditions.the purpose of this study was to develop predictive models for the growth of listeria monocytogenes in pork bulgogi at various storage temperatures. a two-strain mixture of l. monocytogenes (atcc 15313 and isolated from pork bulgogi) was inoculated on pork bulgogi at 3 log cfu/g. l. monocytogenes strains were enumerated using general plating method on listeria selective medium. the inoculated samples were stored at 5, 15, and 25℃ for primary models. primary models were developed using the barany ...201526761807
listeria monocytogenes infection in ruminants: is there a link to the environment, food and human health? a review.listeria (l.) monocytogenes is widely distributed in the environment, but also has the ability to cause serious invasive disease in ruminants and humans. this review provides an overview of listeriosis in ruminants and discusses our insufficient understanding of reservoirs and possible cycling ofl. monocytogenes between animal and human hosts, food and the environment. it indicates gaps in our knowledge of the role of genetic subtypes in l. monocytogenes ecology and virulence as well as risk fac ...201526753347
growth potential of listeria monocytogenes and staphylococcus aureus on fresh-cut tropical fruits.the objective of this study was to evaluate the fate of staphylococcus aureus, listeria monocytogenes, and natural microbiota on fresh-cut tropical fruits (pitaya, mango, papaya and pineapple) with commercial pvc film at different storage temperature (5, 13, and 25 °c). the results showed that s. aureus, l. monocytogenes, and natural microbiota increased significantly on fresh-cut tropical fruits at 25 °c. both pathogen and natural microbiota were able to grow on fresh-cut tropical fruits at 13 ...201526862605
probabilistic models to predict listeria monocytogenes growth at low concentrations of nano2 and nacl in frankfurters.this study developed probabilistic models to describe listeria monocytogenes growth responses in meat products with low concentrations of nano2 and nacl. a five-strain mixture of l. monocytogenes was inoculated in nbye (nutrient broth plus 0.6% yeast extract) supplemented with nano2 (0-141 ppm) and nacl (0-1.75%). the inoculated samples were then stored under aerobic and anaerobic conditions at 4, 7, 10, 12, and 15℃ for up to 60 d. growth response data [growth (1) or no growth (0)] for each comb ...201526877642
development of a multi-pathogen enrichment broth for simultaneous growth of five common foodborne pathogens.the objective of the present study was to formulate a multi-pathogen enrichment broth which could support the simultaneous growth of five common foodborne pathogens (salmonella enterica, staphylococcus aureus, shigella flexneri, listeria monocytogenes and escherichia coli o157:h7). the formulated broth sssle was composed of potassium tellurite, bile salt, lithium chloride, and sodium chloride as growth-inhibitors; glucose, esculin, mannitol and sodium pyruvate as growth-promoters. compared with ...201526782652
invasiveness of listeria monocytogenes strains isolated from animals in poland.animals are important reservoir of listeria monocytogenes, a pathogen causing serious infections in both humans and livestock. however, data on invasiveness of l. monocytogenes strains of animal origin is very scarce. ability of 18 l. monocytogenes strains of animal origin to invade ht-29 cells was investigated. plaque forming assay was used to assess invasiveness and ability of the pathogen to spread in the cell line. almost 40% of l. monocytogenes strains were weakly invasive. it was shown tha ...201526812809
metallothionein differentially affects the host response to listeria infection both with and without an additional stress from cold-restraint.acute stress alters anti-bacterial defenses, but the neuroimmunological mechanisms underlying this association are not yet well understood. metallothionein (mt), a cysteine-rich protein, is a stress response protein that is induced by a variety of chemical, biological, and psychological stressors, and mt has been shown to influence immune activities. we investigated mt's role in the management of anti-bacterial responses that occur during stress, using a c57bl/6 (b6) strain that has targeted dis ...201526267326
the effect of short-time microwave exposures on listeria monocytogenes inoculated onto chicken meat portions.listeria monocytogenes can be found throughout the environment and in many foods. it is associated primarily with meat and animal products. listeria monocytogenes has become increasingly important as a food-borne pathogen. the aim of this study was to evaluate the effect of microwave (mw) treatment of chicken meat samples which were inoculated with l. monocytogenes. drumettes of broiler carcasses were soaked in fully growth of l. monocytogenes in brain-heart infusion broth. the swab samples were ...201526261715
isg15 counteracts listeria monocytogenes infection.isg15 is an interferon-stimulated, linear di-ubiquitin-like protein, with anti-viral activity. the role of isg15 during bacterial infection remains elusive. we show that isg15 expression in nonphagocytic cells is dramatically induced upon listeria infection. surprisingly this induction can be type i interferon independent and depends on the cytosolic surveillance pathway, which senses bacterial dna and signals through sting, tbk1, irf3 and irf7. most importantly, we observed that isg15 expressio ...201526259872
estimates of foodborne illness-related hospitalizations and deaths in canada for 30 specified pathogens and unspecified agents.foodborne illness estimates help to set food safety priorities and create public health policies. the public health agency of canada estimates that 4 million episodes of foodborne illness occur each year in canada due to 30 known pathogens and unspecified agents. the main objective of this study was to estimate the number of domestically acquired foodborne illness-related hospitalizations and deaths. using the estimates of foodborne illness for canada along with data from the canadian hospitaliz ...201526259128
multi-province listeriosis outbreak linked to contaminated deli meat consumed primarily in institutional settings, canada, 2008.a multi-province outbreak of listeriosis occurred in canada from june to november 2008. fifty-seven persons were infected with 1 of 3 similar outbreak strains defined by pulsed-field gel electrophoresis, and 24 (42%) individuals died. forty-one (72%) of 57 individuals were residents of long-term care facilities or hospital inpatients during their exposure period. descriptive epidemiology, product traceback, and detection of the outbreak strains of listeria monocytogenes in food samples and the p ...201526258258
the evolution and epidemiology of listeria monocytogenes in europe and the united states.listeria monocytogenes is an opportunistic food-borne pathogen responsible for listeriosis, a disease associated with high mortality rates. l. monocytogenes causes invasive syndromes and case-fatality can be as high as 30%, in specific high-risk population groups such as the elderly, immuno-compromised individuals, fetuses and newborns. acquisition of the disease is mainly due to consumption of contaminated (predominantly ready-to-eat) food. we aimed to provide a state-of-the-art collection of d ...201526254574
phylogenomic grouping of listeria monocytogenes.the precise delineation of lineages and clonal groups are a prerequisite to examine within-species genetic variations, particularly with respect to pathogenic potential. a whole-genome-based approach was used to subtype and subgroup isolates of listeria monocytogenes. core-genome typing was performed, employing 3 different approaches: total core genes (cg), high-scoring segment pairs (hsps), and average nucleotide identity (ani). examination of 113 l. monocytogenes genomes available in-house and ...201526245135
eradication of high viable loads of listeria monocytogenes contaminating food-contact surfaces.this study demonstrates the efficacy of cold gaseous ozone treatments at low concentrations in the eradication of high listeria monocytogenes viable cell loads from glass, polypropylene, stainless steel, and expanded polystyrene food-contact surfaces. using a step by step approach, involving the selection of the most resistant strain-surface combinations, 11 listeria sp. strains resulted inactivated by a continuous ozone flow at 1.07 mg m(-3) after 24 or 48 h of cold incubation, depending on bot ...201526236306
reconstituting the actin cytoskeleton at or near surfaces in vitro.actin filament dynamics have been studied for decades in pure protein solutions or in cell extracts, but a breakthrough in the field occurred at the turn of the century when it became possible to reconstitute networks of actin filaments, growing in a controlled but physiological manner on surfaces, mimicking the actin assembly that occurs at the plasma membrane during cell protrusion and cell shape changes. the story begins with the bacteria listeria monocytogenes, the study of which led to the ...201526235437
pregnancy - associated human listeriosis: virulence and genotypic analysis of listeria monocytogenes from clinical samples.listeria monocytogenes, a life-threatening pathogen, poses severe risk during pregnancy, may cause abortion, fetal death or neonatal morbidity in terms of septicemia and meningitis. the present study aimed at characterizing l. monocytogenes isolated from pregnant women based on serotyping, antibiotic susceptibility, virulence genes, in vivo pathogenicity test and eric- and rep-pcr fingerprint analyses. the results revealed that out of 3700 human clinical samples, a total of 30 (0.81%) isolates [ ...201526231373
short branched-chain c6 carboxylic acids result in increased growth, novel 'unnatural' fatty acids and increased membrane fluidity in a listeria monocytogenes branched-chain fatty acid-deficient mutant.listeria monocytogenes is a psychrotolerant food borne pathogen, responsible for the high fatality disease listeriosis, and expensive food product recalls. branched-chain fatty acids (bcfas) of the membrane play a critical role in providing appropriate membrane fluidity and optimum membrane biophysics. the fatty acid composition of a bcfa-deficient mutant is characterized by high amounts of straight-chain fatty acids and even-numbered iso fatty acids, in contrast to the parent strain where odd-n ...201526225744
clonogrouping, a rapid multiplex pcr method for identification of major clones of listeria monocytogenes.three multiplex pcr assays were developed to identify the 11 most common listeria monocytogenes clones in clinical and food samples; 270 (95.7%) of 282 strains of serogroups ivb, iib, iia, and iic were identified accurately. this novel tool is a rapid and efficient alternative to multilocus sequence typing for identification of l. monocytogenes clones.201526224848
contribution of endemic listeriosis to spontaneous abortion and stillbirth in a large outdoor-housed colony of rhesus macaques (macaca mulatta).listeria monocytogenes is an endemic agent in the primate population at the california national primate research center and has been associated with both sporadic cases and a general outbreak of pregnancy failures. the primary objective of this study was to verify the incidence of l. monocytogenes-associated abortion and fetal deaths in the center's outdoor breeding colony. in addition, we sought to compare the group of female macaques that presented with listeria-associated abortion with both t ...201526224440
different methods to quantify listeria monocytogenes biofilms cells showed different profile in their viability.listeria monocytogenes is a foodborne pathogen able to adhere and to form biofilms in several materials commonly present in food processing plants. the aim of this study was to evaluate the resistance of listeria monocytogenes attached to abiotic surface, after treatment with sanitizers, by culture method, microscopy and quantitative real time polymerase chain reaction (qpcr). biofilms of l. monocytogenes were obtained in stainless steel coupons immersed in brain heart infusion broth, under agit ...201526221112
differential gene expression of three gene targets among persistent and nonpersistent listeria monocytogenes strains in the presence or absence of benzethonium chloride.persistence of listeria monocytogenes strains in food processing environments remains relatively common but is difficult to control. understanding the basis for such persistence represents an important step in the potential control or eradication of this pathogen from these environments. in this study, reverse transcription pcr was used to determine the relative and absolute expression of selected gene targets (pocr, eutj, and qach) among five persistent and four presumed nonpersistent l. monocy ...201526219372
determination of the thermal inactivation kinetics of listeria monocytogenes, salmonella enterica, and escherichia coli o157:h7 and non-o157 in buffer and a spinach homogenate.produce has been associated with a rising number of foodborne illness outbreaks. while much produce is consumed raw, some is treated with mild heat, such as blanching or cooking. the objectives of this research were to compare the thermal inactivation kinetics of listeria monocytogenes, salmonella enterica, shiga toxin-producing escherichia coli (stec) o157:h7, and non-o157 stec in phosphate-buffered saline (pbs; ph 7.2) and a spinach homogenate and to provide an estimate of the safety of mild h ...201526219359
supporting data for comparative proteomic analysis of listeria monocytogenes atcc 7644 exposed to a sublethal concentration of nisin.here we provide the lc-ms/ms data from a comparative analysis of listeria monocytogenes atcc 7644 treated and non-treated with a sublethal concentration of nisin (10(-3) mg/ml). protein samples were analyzed by multidimensional protein identification technology (mudpit) approach, in an off-line configuration. the raw ms/ms data allowed the detection of 49,591 spectra which resulted in 576 protein identifications. after scaffold validation, 179 proteins were identified with high confidence. a lab ...201526217729
human lymphocytic b-leukemia cell line treatment with the bacterial toxin listeriolysin o and rituximab (anti-cd20 antibody): effects of similar localization of their receptors.small b-cell lymphocytic lymphoma/chronic lymphocytic leukemia, which typically affects elderly people, is a group of conditions that are not clinically uniform. it has been suggested that using the combined activity of the monoclonal antibody anti-cd20 (rituximab) and listeria monocytogenes toxin listeriolysin o (llo) for this condition could produce an enhanced treatment effect. here, we tested the effect of the joint activity of rituximab and llo, which is a cell membrane toxin, in human leuk ...201526216910
behaviour of listeria monocytogenes and escherichia coli o157:h7 during the cheese making of traditional raw-milk cheeses from italian alps.the behaviour of listeria monocytogenes and escherichia coli o157:h7 was studied during the manufacture and ripening of two traditional italian alps cheeses. each cheese type was manufactured in a pilot plan from raw cow milk (without the addition of starter cultures) artificially inoculated with l. monocytogenes and e. coli o157:h7 to a final concentration of about 4 log cfu/ml. the pathogens were enumerated throughout the cheese making and ripening processes to study their behaviour. when the ...201527800408
characterization of epidemic clones of listeria monocytogenes serotype 4b isolated from humans and meat products in brazil.listeria monocytogenes is an important foodborne pathogen and the 4b serotype is responsible for many cases of human listeriosis reported in brazil. several listeriosis outbreaks worldwide have involved a small number of well-defined clonal groups, designated as epidemic clones (ecs).201526409737
behavior of listeria monocytogenes in sliced ready-to-eat meat products packaged under vacuum or modified atmosphere conditions.the objective of this research was to determine the behavior of listeria monocytogenes in three types of sliced ready-to-eat meat products packaged under vacuum or modified atmosphere conditions and stored at three temperatures. slices of about 25 g of chorizo (a fermented dry pork sausage), jamón (cured ham), and cecina (a salted, dried beef product) were inoculated with l. monocytogenes nctc 11994. slices were packaged in a vacuum or in a modified atmosphere (20% co2, 80% n2). after packaging, ...201526408140
effects of two application methods of plantaricin bm-1 on control of listeria monocytogenes and background spoilage bacteria in sliced vacuum-packaged cooked ham stored at 4°c.two application methods were used to investigate the effect of plantaricin bm-1 on the control of listeria monocytogenes and background spoilage bacteria in sliced vacuum-packaged cooked ham without the addition of any chemical preservatives, including sodium nitrite, during 35 days of storage at 4°c. regardless of the application method, plantaricin bm-1 treatment (320, 640, or 1,280 arbitrary units [au]/g of sliced cooked ham) significantly (p < 0.05) reduced the survival of l. monocytogenes ( ...201526408132
prevalence and distribution of listeria monocytogenes inla alleles prone to phase variation and inla alleles with premature stop codon mutations among human, food, animal, and environmental isolates.in listeria monocytogenes, 18 mutations leading to premature stop codons (pmscs) in the virulence gene inla have been identified to date. while most of these mutations represent nucleotide substitutions, a frameshift deletion in a 5' seven-adenine homopolymeric tract (ht) in inla has also been reported. this ht may play a role in phase variation and was first identified among l. monocytogenes lineage ii ribotype dup-1039c isolates. in order to better understand the distribution of different inla ...201526407886
growth of listeria monocytogenes within a caramel-coated apple microenvironment.a 2014 multistate listeriosis outbreak was linked to consumption of caramel-coated apples, an unexpected and previously unreported vehicle for listeria monocytogenes. this outbreak was unanticipated because both the ph of apples (<4.0) and the water activity of the caramel coating (<0.80) are too low to support listeria growth. in this study, granny smith apples were inoculated with approximately 4 log10 cfu of l. monocytogenes (a cocktail of serotype 4b strains associated with the outbreak) on ...201526463161
antilisterial peptides released by enzymatic hydrolysis from grass carp proteins and activity on controlling l. monocytogenes inoculated in surimi noodle.the primary objective of this study was to prepare the antilisterial peptides by enzymatic (pepsin, trypsin, protamex, neutrase, flavourzyme, papain, alcalase, and acid protease) hydrolysis of grass carp proteins with various degree of hydrolysis. the second objective was to evaluate the antilisterial activity of grass carp proteins hydrolysates (gcph) at differnet levels in the surimi noodle samples with or without boiling treatment inoculated with 10(4) cfu/g of listeria monocytogenes for stor ...201526467537
comparison of multiple‑locus variable number tandem repeat analysis and pulsed field gel electrophoresis in molecular subtyping of listeria monocytogenes isolates from italian cheese.subtyping of listeria monocytogenes strains is an essential epidemiological tool to trace contamination and determine evolutionary relationships among different strains. pulsed field gel electrophoresis (pfge) is the current gold standard method for listeria characterization. multiple-locus variable-number tandem repeat analysis (mlva) is a rapid subtyping method based on polymerase chain reaction (pcr) amplification that has been successfully developed for subtyping bacterial genera. the purpos ...201526455371
effect of honokiol on exotoxin proteins listeriolysin o and p60 secreted by listeria monocytogenes.listeria monocytogenes is considered one of the most important foodborne pathogens. the virulence-related proteins listeriolysin o (llo) and p60 are critical factors involved in listeria pathogenesis. in the present study, we investigated the effect of honokiol on llo and p60 secreted from l. monocytogenes. a listeriolysin assay was used to investigate the haemolytic activities of l. monocytogenes exposed to honokiol, and the secretion of llo and p60 was detected by immunoblot analysis. addition ...201526445991
development of listeriabase and comparative analysis of listeria monocytogenes.listeria consists of both pathogenic and non-pathogenic species. reports of similarities between the genomic content between some pathogenic and non-pathogenic species necessitates the investigation of these species at the genomic level to understand the evolution of virulence-associated genes. with listeria genome data growing exponentially, comparative genomic analysis may give better insights into evolution, genetics and phylogeny of listeria spp., leading to better management of the diseases ...201526444974
drosophila melanogaster natural variation affects growth dynamics of infecting listeria monocytogenes.we find that in a listeria monocytogenes/drosophila melanogaster infection model, l. monocytogenes grows according to logistic kinetics, which means we can measure both a maximal growth rate and growth plateau for the microbe. genetic variation of the host affects both of the pathogen growth parameters, and they can vary independently. because growth rates and ceilings both correlate with host survival, both properties could drive evolution of the host. we find that growth rates and ceilings are ...201526438294
a case of listeria monocytogenes endophthalmitis with recurrent inflammation and novel management.listeria monocytogenes is a rare cause of endogenous endophthalmitis. in the limited number of reported listeria endophthalmitis cases, visual acuity outcomes have been very poor.201526437938
[listeria monocytogenes meningitis in immunocompetent children: unpasteurized cheese likely cause of infection].listeria meningoencephalitis is a rare condition, occurring mainly in immunocompromised patients. we present two cases of listeria monocytogenes meningoencephalitis in immunocompetent children, with successful treatment with betalactam/aminoglycoside combination. unpasteurized cheese was postulated as the source of infection.201526436795
characterization of listeria monocytogenes isolated from human listeriosis cases in china. 201526421272
use of e-beam radiation to eliminate listeria monocytogenes from surface mould cheese.camembert and brie soft cheese varieties were subjected to e-beam irradiation as a sanitation treatment. the effects of treatments on microbiota and selected physicochemical properties were also studied. the absorbed doses required to meet the food safety objective (fso) according to eu and usda criteria for listeria monocytogenes were 1.27 and 2.59 kgy, respectively. the bacterial load, mainly lactic acid bacteria, was reduced by the treatment but injured cells were recovered during storage at ...201526415665
polymer-based microfluidic chip for rapid and efficient immunomagnetic capture and release of listeria monocytogenes.infections caused by foodborne pathogens such as listeria monocytogenes pose a threat to public health while timely detection is challenging due to pathogen low numbers. the development of robust and efficient sample preparation techniques is crucial to improve detection sensitivity and workflow. immunomagnetic separation using magnetic nanoparticles (mnps) is attractive, as it can efficiently capture target cells. for food safety applications, a platform is needed to rapidly process large sampl ...201526346021
production of antilisterial bacteriocins from lactic acid bacteria in dairy-based media: a comparative study.one hundred and eight strains of lactic acid bacteria (lab) were screened for bacteriocin production by the modified deferred antagonism and agar well diffusion methods. when the modified deferred antagonism method was employed, 82 lab strains showed inhibitory action against listeria monocytogenes v7 ½a, whereas 26 lab strains expressed no inhibition. only 12 lab strains exhibited inhibitory activity when the agar well diffusion method was used, 11 of which had been previously recognized as bac ...201526341641
estimation of listeria monocytogenes survival during thermoultrasonic treatments in non-isothermal conditions: effect of ultrasound on temperature and survival profiles.estimation of listeria monocytogenes survival during thermoultrasonic treatments in non-isothermal conditions was determined considering an increment from 45 to 70 °c, assessing the adequacy of predictions through experimental data obtained in laboratory media model systems. in order to characterize the sonication effect on the survival pattern, observed behavior was compared to that obtained when only thermal treatment was applied. a noticeable impact on l. monocytogenes survival in non-isother ...201526338125
bacteriophage cocktail significantly reduces or eliminates listeria monocytogenes contamination on lettuce, apples, cheese, smoked salmon and frozen foods.listshield™, a commercially available bacteriophage cocktail that specifically targets listeria monocytogenes, was evaluated as a bio-control agent for l. monocytogenes in various ready-to-eat foods. listshield™ treatment of experimentally contaminated lettuce, cheese, smoked salmon, and frozen entrèes significantly reduced (p < 0.05) l. monocytogenes contamination by 91% (1.1 log), 82% (0.7 log), 90% (1.0 log), and 99% (2.2 log), respectively. listshield™ application, alone or combined with an ...201526338115
erratum: increased spread and replication efficiency of listeria monocytogenes in organotypic brain-slices is related to multilocus variable number of tandem repeat analysis (mlva) complex. 201526335249
antimicrobial poly(lactic acid)-based nanofibres developed by solution blow spinning.the present study reports on the development of hybrid poly(lactic acid) (pla) fibres loaded with highly crystalline bacterial cellulose nanowhiskers (bcnw) by the novel solution blow spinning method. furthermore, fibres with antimicrobial properties were generated by incorporating carvacrol and thc as antimicrobial agents and the biocide effect against listeria monocytogenes was studied. initially, pla blow spun fibres containing bcnw were optimized in terms of morphology and thermal properties ...201526328414
behaviour of listeria monocytogenes in artisanal raw milk pecorino umbro cheese: a microbiological challenge test.in the present study, a microbiological challenge test in artificially contaminated raw milk pecorino umbro cheese during cheese-making was carried out. raw ewe milk was contaminated by a suspension of particular listeria monocytogenes strains. the number of l. monocytogenes and l. monocytogenes dynamic growth were evaluated during cheese-making and storage. a significant decrease of the viable count of l. monocytogenes was observed during ripening and l. monocytogenes viable count was below the ...201527800412
diversity of listeria monocytogenes within a u.s. dairy herd, 2004-2010.listeria monocytogenes, the causative agent of listeriosis, is frequently isolated from the environment. dairy cows and dairy farm environments are reservoirs of this pathogen, where fecal shedding contributes to its environmental dispersal and contamination of milk, dairy products, and meat. the molecular diversity of 40 l. monocytogenes isolates representing 3 serogroups (1/2a, 1/2b, and 4b) collected between 2004 and 2010 from the feces of dairy cattle on a single dairy farm was assessed usin ...201526325149
chloroxyanion residue quantification in cantaloupes treated with chlorine dioxide gas.previous studies show that treatment of cantaloupes with chlorine dioxide (clo2) gas at 5 mg/liter for 10 min results in a significant reduction (p < 0.05) in initial microflora, an increase in shelf life without any alteration in color, and a 4.6- and 4.3-log reduction of escherichia coli o157:h7 and listeria monocytogenes, respectively. however, this treatment could result in the presence of chloroxyanion residues, such as chloride (cl(-)), chlorite (clo2(-)), chlorate (clo3(-)), and perchlora ...201526319725
modeling the effect of storage temperatures on the growth of listeria monocytogenes on ready-to-eat ham and sausage.the aim of this study was to model the growth kinetics of listeria monocytogenes on ready-to-eat ham and sausage at different temperatures (4 to 35°c). the observed data fitted well with four primary models (baranyi, modified gompertz, logistic, and huang) with high coefficients of determination (r(2) > 0.98) at all measured temperatures. after the mean square error (0.009 to 0.051), bias factors (0.99 to1.06), and accuracy factors (1.01 to 1.09) were obtained in all models, the square root and ...201526319721
putative cross-contamination routes of listeria monocytogenes in a meat processing facility in romania.putative routes of listeria monocytogenes contamination, based on the workflow of the employees, were studied in a meat processing facility by investigating 226 samples collected from food contact surfaces, non-food contact surfaces, raw materials, and ready-to-eat meat products on four occasions over a 1-year period. in total, 19.7% of non-food contact surfaces, 22.9% of food contact surfaces, 45% of raw materials, and 20% of ready-to-eat meat products were positive for l. monocytogenes (analyz ...201526319720
effect of alginate coatings with cinnamon bark oil and soybean oil on quality and microbiological safety of cantaloupe.the quality and microbiological safety of cantaloupes can potentially be improved using antimicrobial coatings that are able to maintain effectiveness throughout storage. the objective of this work was to study the effect of coating mixtures containing sodium alginate and cinnamon bark oil (cbo) on the quality of cantaloupes and the survival of inoculated bacterial pathogens and naturally occurring yeasts and molds during ambient storage at 21 °c. cantaloupes were dipped in mixtures containing 1 ...201526318410
listeria monocytogenes prevalence and characteristics in retail raw foods in china.the prevalence and levels of listeria monocytogenes in retail raw foods covering most provincial capitals in china were studied with testing of 1036 samples of vegetables, edible mushrooms, raw meat, aquatic products and quick-frozen products from september 2012 to january 2014. the total prevalence of listeria monocytogenes was 20.0% (207/1036), and the most probable number (mpn) values of 65.7% of the positive samples ranged from 0.3 to 110 mpn/g. geographical differences were observed in this ...201526317852
rhombencephalitis by listeria monocytogens in two diabetic patients.brainstem involvement is a characteristic feature and accounts for the high mortality associated with listeriosis especially in immunosuppressed patients. we report two cases of rhombencephalitis infection by listeria monocytogenes in a 65 and 63-year-old men with diabetes. they were referred to a neurologist due to fever and drowsiness after 3 weeks. the 65-year-old man had vertigo, diplopia, ataxia, bidirectional nystagmus and the 63-year-old man complained of perioral numbness, dysphagia and ...201526317605
pi3kδ regulates the magnitude of cd8+ t cell responses after challenge with listeria monocytogenes.pi3ks regulate diverse immune cell functions by transmitting intracellular signals from ag, costimulatory receptors, and cytokine receptors to control cell division, differentiation, survival, and migration. in this study, we report the effect of inhibiting the p110δ subunit of pi3kδ on cd8(+) t cell responses to infection with the intracellular bacteria listeria monocytogenes. a strong dependency on pi3kδ for ifn-γ production by cd8(+) t cells in vitro was not recapitulated after listeria infec ...201526311905
it is not all about single nucleotide polymorphisms: comparison of mobile genetic elements and deletions in listeria monocytogenes genomes links cases of hospital-acquired listeriosis to the environmental source.the control of food-borne outbreaks caused by listeria monocytogenes in humans relies on the timely identification of food or environmental sources and the differentiation of outbreak-related isolates from unrelated ones. this study illustrates the utility of whole-genome sequencing for examining the link between clinical and environmental isolates of l. monocytogenes associated with an outbreak of hospital-acquired listeriosis in sydney, australia. comparative genomic analysis confirmed an epid ...201526311854
evaluation of two loop-mediated isothermal amplification methods for the detection of salmonella enteritidis and listeria monocytogenes in artificially contaminated ready-to-eat fresh produce.in the present study, the effectiveness of two loop-mediated isothermal amplification (lamp) assays was evaluated. samples of romaine lettuce, strawberries, cherry tomatoes, green onions and sour berries were inoculated with known dilutions (10(0)-10(8) cfu/g of produce) of s. enteritidis and l. monocytogenes. with lamp, assay pathogens can be detected in less than 60 min. the limits of detection of s. enteritidis and l. monocytogenes depended on the food sample tested and on the presence of enr ...201527800413
a comparison analysis of listeria monocytogenes isolates recovered from chicken carcasses and human by using rapd pcr.chicken meat is a valuable source of protein and consumption of it continues to rise day to day. the aim of the present study was to evaluate the genetic homogeneity of listeria monocytogenes (l. monocytogenes) isolates obtained from chicken carcasses and human. random amplification of polymorphic dna (rapd) pcr with three different primers were used to analyze the 31 l. monocytogenes isolates recovered from human and chicken carcasses. primers were d8635, hlwl74, and opm01. scanned images of ra ...201526309714
influence of ph on bile sensitivity amongst various strains of listeria monocytogenes under aerobic and anaerobic conditions.listeria monocytogenes is a dangerous bacterium that causes the food-borne disease listeriosis and accounts for nearly 20% of food-borne deaths. this organism can survive the body's natural defences within the digestive tract, including acidic conditions and bile. although the bile response has been analysed, limited information is available concerning the ability of l. monocytogenes to resist bile under anaerobic conditions, especially at acidic ph, which mimics conditions within the duodenum. ...201526307079
sly1 regulates t-cell proliferation during listeria monocytogenes infection in a foxo1-dependent manner.infection of mice with listeria monocytogenes results in a strong t-cell response that is critical for an efficient defense. here, we demonstrate that the adapter protein sly1 (sh3-domain protein expressed in lymphocytes 1) is essential for the generation of a fully functional t-cell response. the lack of sly1 leads to reduced survival rates of infected mice. the increased susceptibility of sly1 knock-out (ko) mice was caused by reduced proliferation of differentiated t cells. ex vivo analyses o ...201526306874
quantification of listeria monocytogenes cells with digital pcr and their biofilm cells with real-time pcr.the purpose of this study was to develop a pcr-based method for quantification of listeria monocytogenes adhesion in microtitre plates. we optimized isolation of dna in the microtitre plates using cell lysis, ultrasound treatment, heating, and centrifugation of the lysate. digital pcr was applied for quantification of l. monocytogenes dna that was used for construction of the standard curve, and real-time pcr was used for quantification of the attached l. monocytogenes cells. this pcr-based meth ...201526304835
directly observing the lipid-dependent self-assembly and pore-forming mechanism of the cytolytic toxin listeriolysin o.listeriolysin o (llo) is the major virulence factor of listeria monocytogenes and a member of the cholesterol-dependent cytolysin (cdc) family. gram-positive pathogenic bacteria produce water-soluble cdc monomers that bind cholesterol-dependent to the lipid membrane of the attacked cell or of the phagosome, oligomerize into prepores, and insert into the membrane to form transmembrane pores. however, the mechanisms guiding llo toward pore formation are poorly understood. using electron microscopy ...201526302195
designer probiotics: development and applications in gastrointestinal health.given the increasing commercial and clinical relevance of probiotics, improving their stress tolerance profile and ability to overcome the physiochemical defences of the host is an important biological goal. herein, i review the current state of the art in the design of engineered probiotic cultures, with a specific focus on their utility as therapeutics for the developing world; from the treatment of chronic and acute enteric infections, and their associated diarrhoeal complexes, to targeting h ...201526301121
identification of listeria spp. strains isolated from meat products and meat production plants by multiplex polymerase chain reaction.listeriosis is a foodborne disease caused by listeria monocytogenes and is considered as a serious health problem, due to the severity of symptoms and the high mortality rate. recently, other listeria species have been associated with disease in human and animals. the aim of this study was to develop a multiplex polymerase chain reaction (pcr) in order to simultaneously detect six listeria species (l. grayi, l. welshimeri, l. ivanovii, l. monocytogenes, l. seeligeri, l. innocua) in a single reac ...201527800422
dietary effects of oregano (origanum vulgaris l.) plant or sweet chestnut (castanea sativa mill.) wood extracts on microbiological, chemico-physical characteristics and lipid oxidation of cooked ham during storage.the aim of this study was to evaluate the dietary effect of feeding pigs with diets enriched with sweet chestnut wood (castanea sativa mill.) or oregano (origanum vulgaris l.) extract on the microbiological and chemical characteristics of cooked pork ham. three groups of 10 pigs were fed with a control diet (ctrl), with the ctrl diet enriched with 0.2% of oregano extract (or) and with the ctrl diet enriched with 0.2% of sweet chestnut wood extract (scw), respectively. six cooked hams per group w ...201527800421
bacterial meningitis: an update of new treatment options.the outcome of bacterial meningitis critically depends on the rapid initiation of bactericidal antibiotic therapy and adequate management of septic shock. in community-acquired meningitis, the choice of an optimum initial empirical antibiotic regimen depends on the regional resistance patterns. pathogens resistant to antibacterials prevail in nosocomial bacterial meningitis. dexamethasone is recommended as adjunctive therapy for community-acquired meningitis in developed countries. in comatose p ...201526293166
sonorensin: a new bacteriocin with potential of an anti-biofilm agent and a food biopreservative.the emergence of antibiotic resistant bacteria has led to exploration of alternative therapeutic agents such as ribosomally synthesized bacterial peptides known as bacteriocins. biofilms, which are microbial communities that cause serious chronic infections, form environments that enhance antimicrobial resistance. bacteria in biofilm can be upto thousand times more resistant to antibiotics than the same bacteria circulating in a planktonic state. in this study, sonorensin, predicted to belong to ...201526292786
evaluating the effect of copper nanoparticles in inhibiting pseudomonas aeruginosa and listeria monocytogenes biofilm formation.biofilm formation is a major virulence factor in different bacteria. biofilms allow bacteria to resist treatment with antibacterial agents. the biofilm formation on glass and steel surfaces, which are extremely useful surfaces in food industries and medical devices, has always had an important role in the distribution and transmission of infectious diseases.201526290685
the padr-like transcriptional regulator lftr ensures efficient invasion of listeria monocytogenes into human host cells.invasion of the bacterial pathogen listeria monocytogenes into human host cells requires specialized surface molecules for attachment and induction of phagocytosis. however, efficient invasion is also dependent on factors with house-keeping functions, such as seca2-dependent secretion of autolysins for post-divisional segregation of daughter cells. mutations in this pathway prevent degradation of peptidoglycan cross-walls, so that long cell chains are formed that cannot be phagocytosed. the extr ...201526284051
experimental design for the optimization of propidium monoazide treatment to quantify viable and non-viable bacteria in piggery effluents.distinguishing between viable and dead bacteria in animal and urban effluents is a major challenge. among existing methods, propidium monoazide (pma)-qpcr is a promising way to quantify viable cells. however, its efficiency depends on the composition of the effluent, particularly on total suspended solids (tss)) and on methodological parameters. the aim of this study was evaluate the influence of three methodological factors (concentration of pma, incubation time and photoactivation time) on the ...201526276157
effect of rosemary essential oil and modified-atmosphere packaging (map) on meat quality and survival of pathogens in poultry fillets.the effect of rosemary (rosmarinus officinalis l.) essential oil (reo) and modified-atmosphere packaging (map) on the survival of certain pathogens (salmonella typhimurium and listeria monocytogenes) in poultry fillets and on their meat quality during 7 days of refrigerated storage were investigated. because reo at 0.05% and 0.1% had weak antibacterial activity and reo at 0.3%, 0.5% and 1.0% imparted unacceptable organoleptic properties, only reo at 0.2% was used to treat the poultry meat. the r ...201526273279
evaluation of 3m™ molecular detection assay (mda) listeria for the detection of listeria species in selected foods and environmental surfaces: collaborative study, first action 2014.06.the 3m™ molecular detection assay (mda) listeria is used with the 3m molecular detection system for the detection of listeria species in food, food-related, and environmental samples after enrichment. the assay utilizes loop-mediated isothermal amplification to rapidly amplify listeria target dna with high specificity and sensitivity, combined with bioluminescence to detect the amplification. the 3m mda listeria method was evaluated using an unpaired study design in a multilaboratory collaborati ...201526268982
evaluation of 3m™ molecular detection assay (mda) listeria monocytogenes for the detection of listeria monocytogenes in selected foods and environmental surfaces: collaborative study, first action 2014.07.the 3m™ molecular detection assay (mda) listeria monocytogenes combines isothermal amplification and bioluminescence to detect listeria monocytogenes with high specificity and efficiency in select foods and environmental samples. the 3m mda listeria monocytogenes method was evaluated using an unpaired study design in a multilaboratory collaborative study to the u.s. department of agriculture, food safety and inspection service microbiology laboratory guidebook 8.09 (2011) isolation and identific ...201526268981
nosocomial empyema caused by a rare serotype, serotype 4c, of listeria monocytogenes in a patient with rheumatoid arthritis and chronic kidney disease: a case report and review of the literature.the intracellular pathogen listeria monocytogenes presents characteristically as meningoencephalitis and bacteremia. herein, we report an extremely rare case of empyema caused by serotype 4c l. monocytogenes in an immunocompromised patient. this case supports the inclusion of l. monocytogenes infection in the differential diagnosis of empyema in an immunocompromised patient.201526268455
mechanistic model coupling gas exchange dynamics and listeria monocytogenes growth in modified atmosphere packaging of non respiring food.a mechanistic model coupling o2 and co2 mass transfer (namely diffusion and solubilisation in the food itself and permeation through the packaging material) to microbial growth models was developed aiming at predicting the shelf life of modified atmosphere packaging (map) systems. it was experimentally validated on a non-respiring food by investigating concomitantly the o2/co2 partial pressure in packaging headspace and the growth of listeria monocytogenes (average microbial count) within the fo ...201526187845
combined effects of thermosonication and slightly acidic electrolyzed water on the microbial quality and shelf life extension of fresh-cut kale during refrigeration storage.this study evaluated the efficacy of thermosonication combined with slightly acidic electrolyzed water (sacew) on the shelf life extension of fresh-cut kale during storage at 4 and 7 °c. each kale (10 ± 0.2 g) was inoculated to contain approximately 6 log cfu/g of listeria monocytogenes. each inoculated or uninoculated samples was dip treated at 40 °c for 3 min with deionized water, thermosonication (400 w/l), sacew (5 mg/l), sodium chlorite (sc; 100 mg/l), sodium hypochlorite (sh; 100 mg/l), an ...201526187840
microbiological assessment along the fish production chain of the norwegian pelagic fisheries sector--results from a spot sampling programme.microbes play an important role in the degradation of fish products, thus better knowledge of the microbiological conditions throughout the fish production chain may help to optimise product quality and resource utilisation. this paper presents the results of a ten-year spot sampling programme (2005-2014) of the commercially most important pelagic fish species harvested in norway. fish-, surface-, and storage water samples were collected from fishing vessels and processing factories. totally 1,1 ...201526187839
control of listeria species food safety at a poultry food production facility.surveillance and control of food-borne human pathogens, such as listeria monocytogenes, is a critical aspect of modern food safety programs at food production facilities. this study evaluated contamination patterns of listeria species at a poultry food production facility, and evaluated the efficacy of procedures to control the contamination and transfer of the bacteria throughout the plant. the presence of listeria species was studied along the production chain, including raw ingredients, food- ...201526187831
cold plasma treatment for the microbiological safety of cabbage, lettuce, and dried figs.microwave-powered cold plasma treatment (cpt) was evaluated as a means to improve the microbiological safety of fresh vegetables and dried fruits. the cpt at 900 w, conducted for 10 min using nitrogen as a plasma-forming gas, inactivated salmonella typhimurium inoculated on cabbage and lettuce by approximately 1.5 log cfu/g. the cpt at 400-900 w and 667 pa, conducted for 1-10 min using a helium-oxygen gas mixture, inactivated listeria monocytogenes on cabbage by 0.3-2.1 log cfu/g in a time-depen ...201526187830
comparative genomics of listeria sensu lato: genus-wide differences in evolutionary dynamics and the progressive gain of complex, potentially pathogenicity-related traits through lateral gene transfer.historically, genome-wide and molecular characterization of the genus listeria has concentrated on the important human pathogen listeria monocytogenes and a small number of closely related species, together termed listeria sensu strictu. more recently, a number of genome sequences for more basal, and nonpathogenic, members of the listeria genus have become available, facilitating a wider perspective on the evolution of pathogenicity and genome level evolutionary dynamics within the entire genus ...201526185097
cutting edge: a natural antisense transcript, as-il1α, controls inducible transcription of the proinflammatory cytokine il-1α.natural antisense transcripts (nats) are a class of long noncoding rnas (lncrnas) that are complementary to other protein-coding genes. although thousands of nats are encoded by mammalian genomes, their functions in innate immunity are unknown. in this study, we identified and characterized a novel nat, as-il1α, which is partially complementary to il-1α. similar to il-1α, as-il1α is expressed at low levels in resting macrophages and is induced following infection with listeria monocytogenes or s ...201526179904
prfa regulation offsets the cost of listeria virulence outside the host.virulence traits are essential for pathogen fitness, but whether they affect microbial performance in the environment, where they are not needed, remains experimentally unconfirmed. we investigated this question with the facultative pathogen listeria monocytogenes and its prfa virulence regulon. prfa-regulated genes are activated intracellularly (prfa 'on') but shut down outside the host (prfa 'off'). using a mutant prfa regulator locked on (prfa*) and thus causing prfa-controlled genes to be co ...201526178789
the multicopy srna lhrc controls expression of the oligopeptide-binding protein oppa in listeria monocytogenes.listeria monocytogenes is the causative agent of the foodborne disease listeriosis. during infection, l. monocytogenes produces an array of non-coding rnas, including the multicopy srna lhrc. these five, nearly identical srnas are highly induced in response to cell envelope stress and target the virulence adhesin lapb at the post-transcriptional level. here, we demonstrate that lhrc controls expression of additional genes encoding cell envelope-associated proteins with virulence function. using ...201526176322
a sensitive impedance biosensor based on immunomagnetic separation and urease catalysis for rapid detection of listeria monocytogenes using an immobilization-free interdigitated array microelectrode.in this study, we described a novel impedance biosensor combining immunomagnetic separation with urease catalysis for sensitive detection of foodborne bacteria using listeria monocytogenes as model and an immobilization-free microelectrode as detector. the monoclonal antibodies (mabs) were immobilized on the surface of the magnetic nanoparticles (mnps) with the diameter of 180 nm by biotin-streptavidin system for specifically and efficiently separating listeria cells from sample background. the ...201526176211
unusual presentation of listerial myocarditis and the diagnostic value of cardiac magnetic resonance.listeria monocytogenes is an infrequent cause of bacterial myocarditis. myocarditis without evidence of endocarditis is even rarer. management in such cases involves early diagnosis, antibiotic therapy, and emergency treatment of arrhythmias. we report the case of a 47-year-old man who presented with features of acute st-segment-elevation myocardial infarction complicated by ventricular tachycardia that necessitated urgent electrical cardioversion. contrast-enhanced cardiac magnetic resonance im ...201526175642
application of a novel antimicrobial coating on roast beef for inactivation and inhibition of listeria monocytogenes during storage.the antilisterial efficacy of novel coating solutions made with organic acids, lauric arginate ester, and chitosan was evaluated in a three-stage study on inoculated roast beef for the first time. ready-to-eat roast beef was specially ordered from the manufacturer. the meat surface was inoculated with five-strain listeria monocytogenes cocktail inoculums at two different levels, ~3 and 6 log cfu/cm(2) and treated with the stock solution (hams), the 1:5 diluted solution (mams), and the 1:10 dilut ...201526173201
lamellipodin is important for cell-to-cell spread and actin-based motility in listeria monocytogenes.listeria monocytogenes is a foodborne pathogen capable of invading a broad range of cell types and replicating within the host cell cytoplasm. this paper describes the colocalization of host cell lamellipodin (lpd) with intracellular l. monocytogenes detectable 6 h postinfection of epithelial cells. the association was mediated via interactions between both the peckstrin homology (ph) domain in lpd and phosphatidylinositol (3,4)-bisphosphate [pi(3,4)p2] on the bacterial surface and by interactio ...201526169271
fundamental characteristics of deep-uv light-emitting diodes and their application to control foodborne pathogens.low-pressure mercury uv (lp-uv) lamps have long been used for bacterial inactivation, but due to certain disadvantages, such as the possibility of mercury leakage, deep-uv-c light-emitting diodes (duv-leds) for disinfection have recently been of great interest as an alternative. therefore, in this study, we examined the basic spectral properties of duv-leds and the effects of uv-c irradiation for inactivating foodborne pathogens, including escherichia coli o157:h7, salmonella enterica serovar ty ...201526162872
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