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is it safe to reuse dental implant healing abutments sterilized and serviced by dealers of dental implant manufacturers? an in vitro sterility analysis.this study was undertaken to investigate the sterility of used healing abutments sterilized and serviced by dealers of dental implant manufacturers.201525706262
natural sesquiterpene lactones enhance oxacillin and gentamicin effectiveness against pathogenic bacteria without antibacterial effects on beneficial lactobacilli.this is a report on the synergistic interactions (sis) between melampolide-type sesquiterpene lactones 1-8 from acanthospermum hispidum dc., and oxacillin or gentamicin, against four pathogenic strains of staphylococcus aureus and enterococcus faecalis; two of them were multi-resistant strains obtained from chronic infectious processes. our results showed that all associations of 1-8 with antibiotics (atbs) are more effective than pure atbs to control pathogenic strains of s. aureus and e. faeca ...201525641272
antibacterial effect of butyryl alkannin from arnebia euchroma against vancomycin-resistant pathogens of enterococcus faecalis causing urinary tract infections.this study was carried out to investigate the biomedicinal potential of a bioactive marker component, butyryl alkannin, isolated from n-hexane root extract of arnebia euchroma against various vancomycin-resistant enterococcus (vre) isolates of enterococcus faecalis causing urinary tract infections. as a result, butyryl alkannin showed significant antibacterial activity against multidrug-resistant e. faecalis pathogens of vre as minimum inhibitory concentration values which were found in the rang ...201525635653
ef0176 and ef0177 from enterococcus faecalis v583 are substrate-binding lipoproteins involved in abc transporter mediated ribonucleoside uptake.one of the abc transporter systems in enterococcus faecalis v583 is encoded by the ef0176-ef0180 gene cluster, which differs from orthologous operons in related bacteria in that it contains two genes putatively encoding substrate-binding proteins (sbps). these sbps, ef0176 and ef0177, have previously been identified on the surface of e. faecalis. by phenotypic studies of single and double knockout mutants, we show here that ef0176 and ef0177 are specific for ribonucleosides and, by inference, th ...201525635276
in vitro activity of linezolid as assessed through the 2013 leader surveillance program.the 2013 leader surveillance program monitored the in vitro activity of linezolid and comparator agents against gram-positive bacteria at 60 medical centers in the united states. a total of 7183 pathogens were contributed from 6 predetermined pathogen groups. the groups were staphylococcus aureus, coagulase-negative staphylococci, enterococci, streptococcus pneumoniae, β-hemolytic streptococci, and viridans group streptococci. the mic90 value for each of the 6 pathogen groups was 1 μg/ml. suscep ...201525633420
behavior of listeria monocytogenes in a multi-species biofilm with enterococcus faecalis and enterococcus faecium and control through sanitation procedures.the formation of mono-species biofilm (listeria monocytogenes) and multi-species biofilms (enterococcus faecium, enterococcus faecalis, and l. monocytogenes) was evaluated. in addition, the effectiveness of sanitation procedures for the control of the multi-species biofilm also was evaluated. the biofilms were grown on stainless steel coupons at various incubation temperatures (7, 25 and 39°c) and contact times (0, 1, 2, 4, 6 and 8 days). in all tests, at 7°c, the microbial counts were below 0.4 ...201525655573
enterococcus faecalis attenuates the differentiation of macrophages into osteoclasts.enterococcus faecalis is closely associated with refractory apical periodontitis, manifesting periapical lesions and alveolar bone loss. macrophages playing an important role in the induction of inflammation can differentiate into bone-resorbing osteoclasts. in the present study, we investigated the effect of e. faecalis on the differentiation and function of macrophages as osteoclast precursors.201525649294
extraction, characterization and antimicrobial activity of sulfated polysaccharides from fish skins.sulfated polysaccharides were extracted from gray triggerfish (gtsp) and smooth hound (shsp) skins. their chemical and physical characteristics were determined using x-ray diffraction and infrared spectroscopic analysis. the antibacterial activities of gtsp and shsp against listeria monocytogenes (atcc 43251), staphylococcus aureus (atcc 25923), enterococcus faecalis (atcc 29212), escherichia coli (atcc 25922), salmonella enterica (atcc 43972) and enterobacter sp were evaluated by determining cl ...201525647621
antibiofilm efficacy evaluation of a bioelectric dressing in mono- and multi-species biofilms.chronic infections are often related to the formation of single or polymicrobial biofilms, which lead to hard-to-treat infections, difficult wound management, and recurrent infections. in this study, we evaluated in vitro antibiofilm properties of a silver and zinc bioelectric dressing (procellera).201525647504
horizontal transfer of antibiotic resistance from enterococcus faecium of fermented meat origin to clinical isolates of e. faecium and enterococcus faecalis.enterococcus species are part of the normal intestinal flora of a large number of mammals including humans and consequently, they can be used as indicators of faecal contamination in food and water for human consumption. their presence in large numbers in foods may indicate a lapse in sanitation and their ability to serve as a genetic reservoir of transferable antibiotic resistance is of concern. in the present study, enterococcus spp., isolated from commercially fermented meat and human clinica ...201525647243
detection of vancomycin resistances in enterococci within 3 ½ hours.vancomycin resistant enterococci (vre) constitute a challenging problem in health care institutions worldwide. novel methods to rapidly identify resistances are highly required to ensure an early start of tailored therapy and to prevent further spread of the bacteria. here, a spectroscopy-based rapid test is presented that reveals resistances of enterococci towards vancomycin within 3.5 hours. without any specific knowledge on the strain, vre can be recognized with high accuracy in two different ...201525645753
β-lactam combinations with daptomycin provide synergy against vancomycin-resistant enterococcus faecalis and enterococcus faecium.enterococcus faecalis (efc) and enterococcus faecium (efm) are frequently resistant to vancomycin and β-lactams (bls). in vitro data suggest synergy between several bls and glycopeptides or lipopeptides against resistant pathogens. our objective was to conduct combination mic and time-kill experiments to evaluate bl synergy with daptomycin against enterococci.201525645208
distribution of human-specific bacteroidales and fecal indicator bacteria in an urban watershed impacted by sewage pollution, determined using rna- and dna-based quantitative pcr assays.the identification of fecal pollution sources is commonly carried out using dna-based methods. however, there is evidence that dna can be associated with dead cells or present as "naked dna" in the environment. furthermore, it has been shown that rrna-targeted reverse transcription-quantitative pcr (rt-qpcr) assays can be more sensitive than rrna gene-based qpcr assays since metabolically active cells usually contain higher numbers of ribosomes than quiescent cells. to this end, we compared the ...201525326295
baseline activity of telavancin against gram-positive clinical isolates responsible for documented infections in u.s. hospitals (2011-2012) as determined by the revised susceptibility testing method.telavancin had mic50 and mic90 values of 0.03 and 0.06 μg/ml (100.0% susceptible), respectively, against methicillin-resistant and -susceptible staphylococcus aureus. telavancin was active against vancomycin-susceptible enterococcus faecalis (mic50/90, 0.12/0.12 μg/ml; 100% susceptible) and enterococcus faecium (mic50/90, 0.03/0.06 μg/ml), while higher mic values were obtained against vancomycin-resistant e. faecium (mic50/90, 1/2 μg/ml) and e. faecalis (mic50/90, >2/>2 μg/ml). streptococci show ...201525348529
oxidative stress enhances cephalosporin resistance of enterococcus faecalis through activation of a two-component signaling system.enterococcus faecalis is a low-gc gram-positive bacterium, a normal resident of the gastrointestinal (gi) tract, and an important hospital-acquired pathogen. an important risk factor for hospital-acquired enterococcal infections is prior therapy with broad-spectrum cephalosporins, antibiotics that impair cell wall biosynthesis by inhibiting peptidoglycan cross-linking. enterococci are intrinsically resistant to cephalosporins; however, environmental factors that modulate cephalosporin resistance ...201525331701
estimation of cultivable bacterial diversity in the cloacae and pharynx in eurasian griffon vultures (gyps fulvus).in this work, we describe the biodiversity of cloacal and pharynx culture-based bacteria (commensal and pathogenic), in 75 eurasian griffon vultures (gyps fulvus) from two geographic areas. we address the question of whether the cultivable microbiota of vultures is organised into assemblages occurring by chance. in addition, we assess bacterial diversity in both anatomic regions and geographic areas. bacterial diversity was represented by 26 gram-negative and 20 gram-positive genera. the most co ...201525388757
bacteriocin protein bacl1 of enterococcus faecalis targets cell division loci and specifically recognizes l-ala2-cross-bridged peptidoglycan.bacteriocin 41 (bac41) is produced from clinical isolates of enterococcus faecalis and consists of two extracellular proteins, bacl1 and baca. we previously reported that bacl1 protein (595 amino acids, 64.5 kda) is a bacteriolytic peptidoglycan d-isoglutamyl-l-lysine endopeptidase that induces cell lysis of e. faecalis when an accessory factor, baca, is copresent. however, the target of bacl1 remains unknown. in this study, we investigated the targeting specificity of bacl1. fluorescence micros ...201525368300
activity of oritavancin against gram-positive clinical isolates responsible for documented skin and soft-tissue infections in european and us hospitals (2010-13).to assess oritavancin activity in vitro against clinically relevant gram-positive pathogens causing skin and soft-tissue infections (sstis) in european and us hospitals.201525362568
a liar deletion restores susceptibility to daptomycin and antimicrobial peptides in multidrug-resistant enterococcus faecalis.daptomycin is a lipopeptide antibiotic that is used clinically against many gram-positive bacterial pathogens and is considered a key frontline bactericidal antibiotic to treat multidrug-resistant enterococci. emergence of daptomycin resistance during therapy of serious enterococcal infections is a major clinical issue. in this work, we show that deletion of the gene encoding the response regulator, liar (a member of the liafsr system that controls cell envelope homeostasis), from daptomycin-res ...201525362197
genomic annotation for the temperate phage efc-1, isolated from enterococcus faecalis kbl101.the temperate phage efc-1 was newly isolated from a mitomycin-c-induced lysate of enterococcus faecalis kbl101. efc-1 has an isometric head and a long tail. the phage belongs to the family siphoviridae according to its genomic structure and morphology. the phage efc-1 has 40,286 base pairs of double-stranded dna and a g+c content of 35.05 %. bioinformatic analysis of the phage revealed 60 putative open reading frames (orfs). the genome of the temperate phage efc-1 was not significantly similar t ...201525359106
ceftobiprole and ampicillin increase daptomycin susceptibility of daptomycin-susceptible and -resistant vre.the synergistic combination of daptomycin plus ampicillin has proven to be effective against vre including daptomycin-non-susceptible strains. ceftobiprole is a cephalosporin with broad binding affinity for enterococcal pbp subtypes including pbp5. given the synergy between β-lactams and daptomycin against vre, it was of interest to determine whether ceftobiprole offered any synergistic advantage with daptomycin compared with ampicillin.201525304643
definitive structural assessment of enterococcal diheteroglycan.enterococcus faecalis is one of most important nosocomial and often multi-antibiotic resistant pathogens responsible for infections that are difficult to treat. previously, a cell-wall polysaccharide termed diheteroglycan (dhg) was isolated and characterized as a promising vaccine candidate. however, the configuration of its lactic acid (la) residue attached to the galactofuranoside unit was not assessed, although it influences conformation of dhg chain in terms of biological recognition and imm ...201525421964
atomic force microscopy visualization of injuries in enterococcus faecalis surface caused by er,cr:ysgg and diode lasers.to visualize by atomic force microscopy the alterations induced on enterococcus. faecalis surface after treatment with 2 types of laser: erbium chromium:yttrium-scandium-gallium-garnet (er,cr:ysgg) laser and diode laser.201525475770
the influence of the wooden equipment employed for cheese manufacture on the characteristics of a traditional stretched cheese during ripening.the influence of the wooden equipment used for the traditional cheese manufacturing from raw milk was evaluated on the variations of chemico-physical characteristics and microbial populations during the ripening of caciocavallo palermitano cheese. milk from two farms (a, extensive; b, intensive) was processed in traditional and standard conditions. chemical and physical traits of cheeses were affected by the farming system and the cheese making technology, and changed during ripening. content in ...201525475270
enterococcus faecalis pcf10-encoded surface proteins prga, prgb (aggregation substance) and prgc contribute to plasmid transfer, biofilm formation and virulence.enterococcus faecalis pcf10 transfers at high frequencies upon pheromone induction of the prgq transfer operon. this operon codes for three cell wall-anchored proteins - prga, prgb (aggregation substance) and prgc - and a type iv secretion system through which the plasmid is delivered to recipient cells. here, we defined the contributions of the prg surface proteins to plasmid transfer, biofilm formation and virulence using the caenorhabditis elegans infection model. we report that a combination ...201525431047
antimicrobial bioassay-guided fractionation of a methanol extract of eupatorium triplinerve.eupatorium triplinerve vahl (asteraceae), popularly known as japana, is widely used in folk medicine, due its analgesic, anticoagulant, antianorexic, antiparasitic, anthelmintic, sedative, antifungal, and antibacterial properties.201525430540
in vitro and in vivo activity of hyperimmune globulin preparations against multiresistant nosocomial pathogens.we compared different immunoglobulin preparations containing igg (intraglobin/intratect) or a mixture of igg, iga, and igm (pentaglobin) to assess the opsonic and protective efficacy of human immunoglobulin preparations against multiresistent nosocomial pathogens.201525428225
role of secondary metabolites in establishment of the mutualistic partnership between xenorhabdus nematophila and the entomopathogenic nematode steinernema carpocapsae.xenorhabdus nematophila engages in a mutualistic partnership with the nematode steinernema carpocapsae, which invades insects, migrates through the gut, and penetrates into the hemocoel (body cavity). we showed previously that during invasion of manduca sexta, the gut microbe staphylococcus saprophyticus appeared transiently in the hemocoel, while enterococcus faecalis proliferated as x. nematophila became dominant. x. nematophila produces diverse secondary metabolites, including the major water ...201525398871
enterococcus faecalis 6-phosphogluconolactonase is required for both commensal and pathogenic interactions with manduca sexta.enterococcus faecalis is a commensal and pathogen of humans and insects. in manduca sexta, e. faecalis is an infrequent member of the commensal gut community, but its translocation to the hemocoel results in a commensal-to-pathogen switch. to investigate e. faecalis factors required for commensalism, we identified e. faecalis genes that are upregulated in the gut of m. sexta using recombinase-based in vivo expression technology (rivet). the rivet screen produced 113 clones, from which we identif ...201525385794
the polyamine n-acetyltransferase-like enzyme pmve plays a role in the virulence of enterococcus faecalis.we previously showed that the mutant strain of enterococcus faecalis lacking the transcriptional regulator slya is more virulent than the parental strain. we hypothesized that this phenotype was due to overexpression of the second gene of the slya operon, ef_3001, renamed pmve (for polyamine metabolism and virulence of e. faecalis). pmve shares strong homologies with n(1)-spermidine/spermine acetyltransferase enzymes involved in the metabolism of polyamines. in this study, we used an e. faecalis ...201525385793
the two-component system grvrs (etars) regulates ace expression in enterococcus faecalis og1rf.expression of ace (adhesin to collagen of enterococcus faecalis), encoding a virulence factor in endocarditis and urinary tract infection models, has been shown to increase under certain conditions, such as in the presence of serum, bile salts, urine, and collagen and at 46 °c. however, the mechanism of ace/ace regulation under different conditions is still unknown. in this study, we identified a two-component regulatory system grvrs as the main regulator of ace expression under these stress con ...201525385790
an in vivo comparison of internal bacterial colonization in two dental implant systems: identification of a pathogenic reservoir.the aim of this study was to compare internal bacterial colonization in two implant systems, one screw root form (srf) with an external hexagon connection and one plateau root form (prf) with a morse taper internal connection.201525385682
photoactivation of curcumin and sodium hypochlorite to enhance antibiofilm efficacy in root canal dentin.to test the effect of ultrasonic or light activated curcumin and sodium hypochlorite against enterococcus faecalis biofilms in vitro.201525462576
the question of pathogen quantification in disinfected graywater.graywater (gw) reuse for irrigation is recognized as a sustainable solution for water conservation. one of the major impediments to gw reuse is the presence of pathogenic microorganisms. this study monitored three similar on-site gw treatment systems bi-monthly over the course of a year to compare the presence of pathogens and indicators in raw, biologically treated, and biologically treated and disinfected [by chlorine and ultraviolet light (uv)] gw. the systems were designed to allow the testi ...201525437766
photodynamic therapy in root canals contaminated with enterococcus faecalis using curcumin as photosensitizer.the aim of the study was to evaluate the photodynamic therapy (pdt) effect on root canals contaminated with enterococcus faecalis using a light emitting diode (led) light and a curcumin solution (cur) as photosensitizer (ps). eighty root canals from uniradicular human teeth were prepared with protaper universal rotary system and contaminated with e. faecalis for 21 days. they were divided as: gia-pdt (cur, pre-irradiation for 5 + 5 min of irradiation); gib-pdt (cur, pre-irradiation for 5 + 10 mi ...201525502831
removal of total and antibiotic resistant bacteria in advanced wastewater treatment by ozonation in combination with different filtering techniques.elimination of bacteria by ozonation in combination with charcoal or slow sand filtration for advanced sewage treatment to improve the quality of treated sewage and to reduce the potential risk for human health of receiving surface waters was investigated in pilot scale at the sewage treatment plant eriskirch, baden-wuerttemberg/germany. to determine the elimination of sewage bacteria, inflowing and leaving wastewater of different treatment processes was analysed in a culture-based approach for ...201525497174
transfer of tetracycline resistance genes with aggregation substance in food-borne enterococcus faecalis.enterococcus faecalis has the ability to conjugate with the aid of aggregation substance (as) and inducible sex pheromones to exchange genetic elements in food matrix. to evaluate the food safety condition and the transferable factor, 250 tetracycline-resistant food-borne e. faecalis were collected in korea. among the isolates, a majority of tetracycline-resistant isolates (49.6 %) harbored both the tet(m) and tet(l) genes together, followed by tet(m) (19.6 %), and tet(l) (6.8 %) alone. also, we ...201525487115
outcome of aortic valve replacement for active infective endocarditis in patients on chronic hemodialysis.the high risk of morbidity and mortality for patients on hemodialysis who are undergoing cardiac surgery is increased for those with active infective endocarditis (aie). this retrospective observational single-center study evaluated the impact of chronic hemodialysis on the outcome of aortic valve replacement in patients with aortic aie.201525483000
biosynthesis of silver nanoparticles using ethanolic petals extract of rosa indica and characterization of its antibacterial, anticancer and anti-inflammatory activities.the present study was aimed at biosynthesis of silver nanoparticles (agnps) using ethanolic extract of rose (rosa indica) petals and testing their potential antibacterial activity using selective human pathogenic microbes, anticancer activity using human colon adenocarcinoma cancer cell line hct 15 as well as anti-inflammatory activity using rat peritoneal macrophages in vitro. the biologically synthesized agnps were also characterized by uv-visible spectroscopy, scanning electron microscopy (se ...201525481491
the use of the replication region of plasmid prs7 from oenococcus oeni as a putative tool to generate cloning vectors for lactic acid bacteria.a chimeric plasmid, prs7rep (6.1 kb), was constructed using the replication region of prs7, a large plasmid from oenococcus oeni, and pem64, a plasmid derived from pij2925 and containing a gene for resistance to chloramphenicol. prs7rep is a shuttle vector that replicates in escherichia coli using its pij2925 component and in lactic acid bacteria (lab) using the replication region of prs7. high levels of transformants per µg of dna were obtained by electroporation of prs7rep into pediococcus aci ...201525479060
effectiveness of ktp laser versus 980 nm diode laser to kill enterococcus faecalis in biofilms developed in experimentally infected root canals.this study aimed to evaluate the antibacterial action of ktp (potassium-titanyl-phosphate) laser irradiations (compared with 980 nm diode laser), associated with conventional endodontic procedures, on enterococcus faecalis biofilms. fifty-six dental roots with single canals were prepared with ni-ti rotary instruments, autoclaved, inoculated with an e. faecalis suspension and incubated for 72 h. they were randomly allocated to control and treatment groups. laser parameters were as follows: power ...201524588799
comparative cell attachment, cytotoxicity and antibacterial activity of radiopaque dicalcium silicate cement and white-coloured mineral trioxide aggregate.to comparatively examine the cell attachment, cytotoxicity, and antibacterial activity of radiopaque dicalcium silicate cement (rdsc) and proroot white-coloured mineral trioxide aggregate (wmta).201524802368
demography and burden of care associated with patients readmitted for urinary tract infection.urinary tract infection (uti) is one of the most prevalent admission diagnoses in hospital-based clinical practice. despite its frequency, few data are available regarding its demographics and economic implications.201524863498
formulation and characterization of lyophilized curcumin solid dispersions for antimicrobial photodynamic therapy (apdt): studies on curcumin and curcuminoids lii.bacterial resistance to antibiotics is increasing and alternative antibacterial treatments like antimicrobial photodynamic therapy (apdt) are needed. curcumin is under investigation as a potential photosensitizer in apdt.201524842546
clonal diversity in biofilm formation by enterococcus faecalis in response to environmental stress associated with endodontic irrigants and medicaments.to determine whether clonal diversity within e. faecalis affects biofilm formation when exposed to antimicrobial compounds found in endodontic medicaments and irrigants.201524749689
in vitro efficacy of antibiotic beads in treating abdominal vascular graft infections.abdominal aortic vascular graft infection often involves several different organisms. antibiotic polymethyl methacrylate (pmma) beads may be effective in controlling infection after débridement, but bacterial species identification and antibiotic susceptibility are often not available at the time of operation, generating a need for a broad-spectrum drug combination for empirical use. we sought to determine an effective antibiotic in pmma beads for use in abdominal vascular graft infection.201524745942
cytotoxicity, interaction with dentine and efficacy on multispecies biofilms of a modified salt solution intended for endodontic disinfection in a new in vitro biofilm model.to investigate the cytotoxicity of a modified salt solution (mss) and evaluate the antimicrobial properties of mss on in vitro biofilm models.201524702260
effectiveness of a new method of disinfecting the root canal, using er, cr:ysgg laser to kill enterococcus faecalis in an infected tooth model.some lasers have demonstrated to provide effective disinfection when used as adjunctive device to the conventional treatment. the aim of this in vitro study was to determine the effectiveness of the erbium, chromium:yttrium scandium gallium garnet (er, cr:ysgg) laser by measuring its bactericidal effect inside the root canal experimentally colonized with enterococcus faecalis. the laser was tested at different irradiation times (30 and 60 s) and energy of impulses (75 and 25 mj). a total of 52 s ...201523917414
antibacterial, antioxidant, anti-cholinesterase potential and flavonol glycosides of biscutella raphanifolia (brassicaceae).different extracts of the aerial parts of biscutella raphanifolia (brassicaceae), which has not been the subject of any study, were screened for the phytochemical content, anti-microbial, antioxidant and anti-cholinesterase activities. we used four methods to identify the antioxidant activity namely, abts(•+), dpph• scavenging, cuprac and ferrous-ions chelating methods. since there is a relationship between antioxidants and cholinesterase enzyme inhibitors, we used two methods to determine the i ...201525553679
[case of a 14-year-old boy with chronic tubulointerstitial nephritis first diagnosed as acute focal bacterial nephritis].a 14-year-old boy was admitted to a general hospital because of prolonged fever of unknown origin. after enterococcus feacalis was detected from his urine and abdominal contrast enhanced computed tomography and 99m-tc dimercaptosuccinic acid scintigram showed multiple focal defects, he was diagnosed as acute focal bacterial nephritis (afbn). his condition recovered as a result of ampicillin (abpc)and cefotaxime infusion. there was no specific finding in voiding cystography. six months later, his ...201525735087
effects of bacterial products on the activity of odontoblast-like cells and their formation of type 1 collagen.to study how products released from different bacteria in a deep carious lesion affect the metabolic activity of odontoblast-like cells and their ability to produce the major organic component of dentine, collagen 1.201423902462
antimicrobial activity of a uv-stable bacteriocin-like inhibitory substance (blis) produced by enterococcus faecium strain dsh20 against vancomycin-resistant enterococcus (vre) strains.the narrow spectrum of action of most bacteriocins is an important limitation for their application as antimicrobial agents. the current study describes a novel bacteriocin-like inhibitory substance (blis) that display extended spectrum antimicrobial activity against vancomycin-resistant enterococcus (vre) strains.201423838264
isolation, identification, and antibiogram of enterococci isolated from patients with urinary tract infection.enterococci, though commensals in adult feces are important nosocomial pathogens. the most common nosocomial infection caused by these organisms is urinary tract infection.201424005591
emergence of enterococcus species in the infectious microorganisms cultured from patients with endophthalmitis in south korea.to investigate the microorganisms in culture-proven endophthalmitis and their susceptibilities to antimicrobial agents commonly used in south korea.201424072645
american crows as carriers of vancomycin-resistant enterococci with vana gene.we studied the vana-carrying vancomycin-resistant enterococci (vre) isolated from american crows in the united states during the winter 2011/2012. faecal samples from crows were cultured selectively for vre and characterized. pulsed-field gel electrophoresis (pfge) and multilocus sequence typing (mlst) were used to examine epidemiological relationships of vana-containing vre. isolates were tested in vitro for their ability to horizontally transfer the vancomycin resistance trait. vre with the va ...201423919480
interdigitated silver-polymer-based antibacterial surface system activated by oligodynamic iontophoresis - an empirical characterization study.there is a pressing need to control the occurrences of nosocomial infections due to their detrimental effects on patient well-being and the rising treatment costs. to prevent the contact transmission of such infections via health-critical surfaces, a prophylactic surface system that consists of an interdigitated array of oppositely charged silver electrodes with polymer separations and utilizes oligodynamic iontophoresis has been recently developed. this paper presents a systematic study that em ...201423917746
oral application of bacterial lysate in infancy diminishes the prevalence of atopic dermatitis in children at risk for atopy.numerous interventions such as avoidance of food allergens, prolonged breast feeding and supplementation of pro-and/or prebiotics have been tried as primary prevention of atopic dermatitis. recent data suggest that prevention of infantile eczema is possible in a subgroup of children by feeding bacterial lysates early in life. bacterial lysates of escherichia coli and enterococcus faecalis were found to impair allergic immune responses in rats. an interventional trial in 606 infants at risk for a ...201423886978
molecular characterization of rifr mutations in enterococcus faecalis and enterococcus faecium.mutation rate is an important factor affecting the appearance and spread of acquired antibiotic resistance. the frequencies and types of enterococci mutations were determined in this study. the mics of rifampicin in enterococci and their rifampicin-resistant mutants were determined by the clinical and laboratory standards institute (clsi) agar dilution method. the enterococcus faecalis isolates a15 and 18165 showed no significant differences in mutation frequencies or mutation rates. in enteroco ...201424070269
apical extrusion of bacteria when using reciprocating single-file and rotary multifile instrumentation systems.to evaluate ex vivo, apical bacterial extrusion associated with two reciprocating single-file systems (waveone and reciproc) compared with a conventional multifile rotary system (biorace).201424111671
isolation and partial characterization of bacteria (pseudoalteromonas sp.) with potential antibacterial activity from a marine costal environment from new caledonia.marine bacteria are a rich source of bioactive metabolites. however, the microbial diversity of marine ecosystem still needs to be explored. the aim of this study was to isolate and characterize bacteria with antimicrobial activities from various marine coastal environment of new caledonia. we obtained 493 marine isolates from various environments and samples of which 63 (12.8%) presented an antibacterial activity against a panel of reference pathogenic strains (staphylococcus aureus, pseudomona ...201424106876
endogenous endophthalmitis caused by enterococcus faecalis in a cat.a three-year-old male neutered domestic shorthair cat was presented for loss of vision associated with hyperthermia, lethargy and anorexia. ophthalmic examination revealed a bilateral panuveitis. cytological examination of aqueous and vitreous humours was performed and revealed a suppurative inflammation associated with numerous cocci. enterococcus faecalis was identified by bacterial culture from aqueous and vitreous humour. no primary infection site was identified. active uveitis resolved afte ...201424028653
reduction in enteroccocus faecalis counts - a comparison between rotary and reciprocating systems.to compare the chemomechanical reduction and regrowth of enterococcus faecalis between rotary and reciprocating systems in root canal preparation.201423980811
efficacy of endodontic applications of ozone and low-temperature atmospheric pressure plasma on root canals infected with enterococcus faecalis.this study aimed to compare the antimicrobial efficacy of low-temperature atmospheric pressure plasma (ltapp) design and gaseous ozone delivery system with 2.5% naocl on enterococcus faecalis in root canal walls and dentine tubules. the samples were divided into ltapp (n = 12), ozone (n = 12), naocl (positive control, n = 12) and saline (negative control, n = 6) groups. microbial samples were collected using paper points and dentin chips from root canals. antimicrobial efficacy was assessed by c ...201423980743
marked elevation of interleukin-6 in mild encephalopathy with a reversible splenial lesion (mers) associated with acute focal bacterial nephritis caused by enterococcus faecalis.this report describes two cases of mild encephalitis/encephalopathy with reversible splenial lesion (mers) associated with acute focal bacterial nephritis (afbn). the patients, who presented with fever and delirious behavior, exhibited hyponatremia and markedly elevated interleukin (il)-6 in cerebrospinal fluid (csf) and serum. enterococcus faecalis was detected in the urine culture. after ampicillin treatment, their consciousness improved without neurological sequelae. moreover, a diffusion-wei ...201423978488
decolourisation of acid orange 7 recalcitrant auto-oxidation coloured by-products using an acclimatised mixed bacterial culture.this study focuses on the biodegradation of recalcitrant, coloured compounds resulting from auto-oxidation of acid orange 7 (ao7) in a sequential facultative anaerobic-aerobic treatment system. a novel mixed bacterial culture, bac-zs, consisting of brevibacillus panacihumi strain zb1, lysinibacillus fusiformis strain zb2, and enterococcus faecalis strain zl bacteria were isolated from environmental samples. the acclimatisation of the mixed culture was carried out in an ao7 decolourised solution. ...201424293297
incidence of virulence factors in enterococci from raw and fermented meat and biofilm forming capacity at 25°c and 37°c.twenty-nine enterococcus strains from raw and fermented meat products were screened for the presence of virulence genes, including those for aggregation substances (asa1 and asa373), cytolysin activator (cyla), collagen binding protein (ace), endocarditis antigen (efaa), enterococcal surface protein (esp) and gelatinase (gele). virulence gene occurrence, expression of gelatinase and pheromone aggregation was greater in enterococcus faecalis than in enterococcus faecium strains. all e. faecalis a ...201424291183
characterization of lactic acid bacteria isolated from infant faeces as potential probiotic starter cultures for fermented sausages.a total of 109 lactic acid bacteria isolated from infant faeces were identified by partial 16s rrna, cpn60 and/or phes sequencing. lactobacillus was the most prevalent genus, representing 48% of the isolates followed by enterococcus (38%). lactobacillus gasseri (21%) and enterococcus faecalis (38%) were the main species detected. a further selection of potential probiotic starter cultures for fermented sausages focused on lactobacillus as the most technologically relevant genus in this type of p ...201424290655
nutritional control of antibiotic resistance via an interface between the phosphotransferase system and a two-component signaling system.enterococci are ubiquitous inhabitants of the gastrointestinal (gi) tract. however, antibiotic-resistant enterococci are also major causes of hospital-acquired infections. enterococci are intrinsically resistant to cephalosporins, enabling growth to abnormally high densities in the gi tract in patients during cephalosporin therapy, thereby promoting dissemination to other sites where they cause infection. despite its importance, many questions about the underlying basis for cephalosporin resista ...201424277024
investigation of a multiresistance gene cfr that fails to mediate resistance to phenicols and oxazolidinones in enterococcus faecalis.to investigate the basis of susceptibility to phenicols and oxazolidinones of the porcine enterococcus faecalis cppf5 despite the presence of the multiresistance gene cfr.201424272266
friends group: clinical and microbiological characteristics of post-filtering surgery endophthalmitis.to study the clinical and microbiological characteristics as well as the prognostic factors for post-filtering surgery endophthalmitis.201424248809
boosted rat natural xenoantibodies cross-react with enterococcus faecalis by targeting melibiose and l-rhamnose.natural antibodies include a subset described as xenoantibodies considered to be directed at microorganisms and also cross-react with antigens of unrelated species. in this study, we generated t-cell-independent (ti) and t-cell-dependent (td) xenoantibodies in lewis rats with hamster and pig blood injections. ti anti-hamster and anti-pig igm and igg xenoantibodies cross-reacted with enterococcus faecalis but not with escherichia coli isolated from the blood of lewis rats after cecal ligation and ...201424246417
biofilm mediates enterococcus faecalis adhesion, invasion and survival into bovine mammary epithelial cells.we proposed in this study that during intramammary infection, biofilm formation may facilitate adherence and colonization of enterococcus faecalis to mammary gland epithelium. this was established by comparing six different ent. faecalis isolates with different biofilm-forming profiles for their adhesive, invasive and survival capabilities to bovine mammary epithelial cell line (mac-t). our results showed increased ability of the biofilm-producer ent. faecalis strains to adhere, invade and survi ...201424224825
immunostimulatory cpg motifs in the genomes of gut bacteria and their role in human health and disease.toll-like receptor (tlr) signalling plays an important role in epithelial and immune cells of the intestine. tlr9 recognizes unmethylated cpg motifs in bacterial dna, and tlr9 signalling maintains the gut epithelial homeostasis. here, we carried out a bioinformatic analysis of the frequency of cpg motifs in the genomes of gut commensal bacteria across major bacterial phyla. the frequency of potentially immunostimulatory cpg motifs (all cpg hexamers) or purine-purine-cg-pyrimidine-pyrimidine hexa ...201424255136
lipoteichoic acid of streptococcus mutans interacts with toll-like receptor 2 through the lipid moiety for induction of inflammatory mediators in murine macrophages.streptococcus mutans is a pathogenic gram-positive bacterium that is closely associated with dental caries and subsequent pulpal inflammation. although lipoteichoic acid (lta) is considered a major virulence factor of gram-positive bacteria, little is known about the innate immunity to s. mutans lta. in this study, we purified lta from s. mutans (sm.lta) through n-butanol extraction, hydrophobic interaction column chromatography, and ion-exchange column chromatography to investigate its immunolo ...201424216318
photoactivated rose bengal functionalized chitosan nanoparticles produce antibacterial/biofilm activity and stabilize dentin-collagen.treatment of infected teeth presents two major challenges: persistence of the bacterial-biofilm within root canals after treatment and compromised structural integrity of the dentin hard-tissue. in this study bioactive polymeric chitosan nanoparticles functionalized with rose-bengal, csrbnp were developed to produce antibiofilm effects as well as stabilize structural-integrity by photocrosslinking dentin-collagen. csrbnp were less toxic to fibroblasts and had significant antibacterial activity e ...201424200522
prevalence of the aminoglycoside phosphotransferase genes aph(3')-iiia and aph(3')-iia in escherichia coli, enterococcus faecalis, enterococcus faecium, pseudomonas aeruginosa, salmonella enterica subsp. enterica and staphylococcus aureus isolates in austria.the aminoglycoside phosphotransferase aph(3')-iia primarily inactivates kanamycin and neomycin, whilst aph(3')-iiia also inactivates amikacin. the aim of this study was to determine the frequency of both resistance genes in major human pathogens to obtain their baseline prevalence in the gene pool of these bacterial populations in austria. in total, 10 541 escherichia coli, enterococcus faecalis, enterococcus faecium, pseudomonas aeruginosa, salmonella enterica subsp. enterica and staphylococcus ...201424194558
sunlight mediated inactivation mechanisms of enterococcus faecalis and escherichia coli in clear water versus waste stabilization pond water.escherichia coli and enterococci have been previously reported to differ in the mechanisms and conditions that affect their sunlight-mediated inactivation in waste stabilization ponds. this study was undertaken to further characterize these mechanisms, using simulated sunlight and single strains of laboratory-grown e. coli and enterococcus faecalis, with a focus on characterizing the contribution of exogenous reactive oxygen species to the inactivation process. we found that direct damage by uvb ...201424188579
vancomycin-resistant enterococci among haemodialysis patients in portugal: prevalence and molecular characterization of resistance, virulence and clonality.vancomycin-resistant enterococci (vre) among haemodialysis patients has increased rapidly and, to date, there is no report of this incidence in portugal.201424169320
a new antibacterial amino phenyl pyrrolidone derivative from a novel marine gliding bacterium rapidithrix thailandica.a recently described marine gliding bacterium rapidithrix thailandica strain tistr 1741 was isolated from biofilm specimen collected from the andaman sea in thailand. four liters fermentation broth of r. thailandica tistr 1741 cultivated in vy/2 medium were extracted with methanol to yield a novel amino phenyl pyrrolidone derivative compound (1) with antibacterial activities. the chemical structure and physico-chemical properties of 1 were investigated by spectrometry techniques. compound 1 exhi ...201424162950
activities of fosfomycin and rifampin on planktonic and adherent enterococcus faecalis strains in an experimental foreign-body infection model.enterococcal implant-associated infections are difficult to treat because antibiotics generally lack activity against enterococcal biofilms. we investigated fosfomycin, rifampin, and their combinations against planktonic and adherent enterococcus faecalis (atcc 19433) in vitro and in a foreign-body infection model. the mic/mbclog values were 32/>512 μg/ml for fosfomycin, 4/>64 μg/ml for rifampin, 1/2 μg/ml for ampicillin, 2/>256 μg/ml for linezolid, 16/32 μg/ml for gentamicin, 1/>64 μg/ml for va ...201424145537
nonclinical and clinical enterococcus faecium strains, but not enterococcus faecalis strains, have distinct structural and functional genomic features.certain strains of enterococcus faecium and enterococcus faecalis contribute beneficially to animal health and food production, while others are associated with nosocomial infections. to determine whether there are structural and functional genomic features that are distinct between nonclinical (nc) and clinical (cl) strains of those species, we analyzed the genomes of 31 e. faecium and 38 e. faecalis strains. hierarchical clustering of 7,017 orthologs found in the e. faecium pangenome revealed ...201424141120
microbiological findings of vulvovaginitis in prepubertal girls.to compare vaginal culture results between prepubertal girls with and without vulvovaginitis, and obtain an overview of the most commonly encountered microbes.201424191064
antibacterial, physicochemical and mechanical properties of endodontic sealers containing quaternary ammonium polyethylenimine nanoparticles.to evaluate the antibacterial, physicochemical and mechanical properties of two endodontic sealers incorporating quaternary ammonium polyethylenimine (qpei) nanoparticles at concentrations of 1% and 2% (w/w).201424134748
isolation and biological activities of an endophytic mortierella alpina strain from the antarctic moss schistidium antarctici.the antarctic endophytic fungus (strain ita1-ccma 952) was isolated from the moss schistidium antarctici found in admiralty bay, king george island, antarctica. strain ita1-ccma 952 was assigned to the specie mortierella alpina by phylogenetic analysis based on 18s rrna gene sequences. this strain produces high levels of polyunsaturated fatty acids (pufas), including y-(gamma) linolenic acid and arachidonic acid, which when combined represents 48.3% of the total fatty acid content. fungal extrac ...201424126742
polymerase chain reaction for enterococcus faecalis in drain fluid: the first screening test for symptomatic colorectal anastomotic leakage. the appeal-study: analysis of parameters predictive for evident anastomotic leakage.with current diagnostic methods, the majority of patients with symptomatic colorectal anastomotic leakage(cal) is identified approximately 1 week after operation.the aim of this study is to determine whether real-time polymerase chain reaction (rt-pcr) for detection of escherichia coli and enterococcus faecalis on drain fluid can serve as a screening test for cal in the early postoperative phase.201424122105
antimicrobial activity of short- and medium-term applications of polyhexamethylene biguanide, chlorhexidine digluconate and calcium hydroxide in infected immature bovine teeth in vitro.to compare the antimicrobial activity of polyhexamethylene biguanide (prontosan wound gel, pr) and chlorhexidine digluconate (chx) after short- and medium-term application with the disinfection ability of calcium hydroxide (ca) in a model using immature bovine teeth.201424118966
hydrolysates of glycated and heat-treated peanut 7s globulin (ara h 1) modulate human gut microbial proliferation, survival and adhesion.evaluation of an effect of glycation of ara h 1 on proliferation and survival rate and adhesion of intestinal enterococcus faecalis, escherichia coli and lactobacillus acidophilus.201424118877
solidago, orthosiphon, birch and cranberry extracts can decrease microbial colonization and biofilm development in indwelling urinary catheter: a microbiologic and ultrastructural pilot study.plants extracts are used in urology to manage urinary tract infections. we aimed to evaluate the efficacy of a preparation with solidago, orthosiphon, birch and cranberry extracts (cistimev plus(®)) in reducing microbial colonization and biofilm development in patients with indwelling urinary catheters.201424092275
in vitro efficacy of various topical antimicrobial agents in different time periods from contamination to application against 6 multidrug-resistant bacterial strains isolated from burn patients.in vitro efficacy evaluation of eleven topical antimicrobials against multidrug-resistant (mdr) bacteria isolated from burn wounds of our patients.201424091332
hospital acquired infections among patients admitted in the medical and surgical wards of a non-teaching secondary care hospital in northern india.to investigate the incidence of nosocomial infection (ni) and type of bacteriological isolates among the patients admitted in the medical and surgical wards of a non-teaching secondary care hospital in north india.201424701489
transcriptome analysis of enterococcus faecalis toward its adaption to surviving in the mouse intestinal tract.we have performed a transcriptomic in vivo study with enterococcus faecalis og1rf in the intestine of living mice to identify novel latent and adaptive fitness determinants within e. faecalis. from 2,658 genes that are present in e. faecalis strain og1rf, 124 genes were identified as significantly differentially expressed within the intestinal tract of living mice as compared to exponential growth in bhi broth. the groups of significantly up- or down-regulated genes consisted of 94 and 30 genes, ...201424700373
antimicrobial prenylated benzoylphloroglucinol derivatives and xanthones from the leaves of garcinia goudotiana.bioassay-guided fractionation using antimicrobial assay of the crude acetonic extract of garcinia goudotiana leaves and of its five partitions led to the isolation of two new prenylated benzoylphloroglucinol derivatives, goudotianone 1 (1) and goudotianone 2 (2), in addition to two known compounds including one xanthone, 1,3,7-trihydroxy-2-isoprenylxanthone (3), and one triterpenoid, friedelin (4). their structures were elucidated on the basis of different spectroscopic methods, including extens ...201424690454
the ability of triple antibiotic paste and calcium hydroxide in disinfection of dentinal tubules.the purpose of this in vitro study was to compare the ability of triple antibiotic paste (tap) to calcium hydroxide (ch) in disinfecting dentinal tubules.201424688581
antimicrobial effect of lippia sidoides and thymol on enterococcus faecalis biofilm of the bacterium isolated from root canals.the species lippia sidoides cham. (verbenaceae) is utilized in popular medicine as a local antiseptic on the skin and mucosal tissues. enterococcus faecalis is the bacterium isolated from root canals of teeth with persistent periapical lesions and has the ability to form biofilm, where it is responsible for the failure of endodontic treatments. essential oil of l. sidoides (eols) and its major component, thymol, were evaluated for reducing the cfu in biofilms of e. faecalis in vitro. the essenti ...201424683344
heterologous expression and characterization of tyrosine decarboxylase from enterococcus faecalis r612z1 and enterococcus faecium r615z1.tyrosine decarboxylase (tdc) is responsible for tyramine production and can catalyze phenylalanine to produce β-phenylethylamine. enterococcus strains are a group of bacteria predominantly producing tyramine and β-phenylethylamine in water-boiled salted duck. in this study, the heterologous expression and characterization of two tdcs from enterococcus faecalis r612z1 (612tdc) and enterococcus faecium r615z1 (615tdc) were studied. the recombinant putative proteins of 612tdc and 615tdc were hetero ...201424680070
influence of in vitro gastrointestinal digestion of fruit juices enriched with pine bark extract on intestinal microflora.the selective antimicrobial effect of fruit juices enriched with pine bark extract (pbe) (0.5 g/l) has been studied before and after in vitro gastrointestinal digestion. pbe (a concentrate of water-soluble bioflavonoids, mainly including phenolic compounds) has been proven to have high stability to the digestion process. pure phenolic compounds such as gallic acid had a high antimicrobial effect on staphylococcus aureus and escherichia coli, maintaining the lactic acid bacteria population (≈100% ...201424679746
identification of temporal clusters and risk factors of bacteremia by nosocomial vancomycin-resistant enterococci.this study aimed to evaluate a different methodology for addressing the evolution of nosocomial bacteremia by vancomycin-resistant enterococci (vre) in a hospital setting.201424679566
antimicrobial resistance and in vitro biofilm-forming ability of enterococci spp. isolated from urinary tract infection in a tertiary care hospital in dhaka.the biofilm mode of life conveys a survival advantage to the microorganism associated with it. biofilm on an indwelling urinary catheter consists of adherent microorganisms, their extra cellular products, and host components deposited on the catheter and thus biofilm on urinary catheters results in persistent infections that are resistant to antimicrobial therapy. this study was done during the period of january 2010 to december 2010. fifty nine enterococci isolated from 1203 urine samples were ...201426118165
in vitro comparison of the effects of probiotic, commensal and pathogenic strains on macrophage polarization.macrophages are important with respect to both innate and adaptive immune responses and are known to differentiate into pro-inflammatory m1- or anti-inflammatory m2-phenotypes following activation. in order to study how different bacteria affect macrophage polarization, we exposed murine raw 264.7 macrophages to sixteen different strains representing probiotic strains, pathogens, commensals and strains of food origin. increased inducible nitric oxide synthase (inos) or arginase-1 gene expression ...201424676762
draft genome sequence of a new homofermentative, lactic acid-producing enterococcus faecalis isolate, cbrd01.we report here the draft genome sequence of the novel homofermentative enterococcus faecalis isolate cbrd01, which is capable of high lactic acid productivity and yields, with minimal nutritional requirements. the genome is 2.8 mbp, with 37% g+c, and contains genes for two lactate dehydrogenase (ldh) enzymes found in related organisms.201424675849
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