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cecal and fecal bacterial flora of the mongolian gerbil and the chinchilla.the mongolian gerbil is being increasingly used as a laboratory animal and as a pet. both chinchillas and gerbils are used as animal models for otitis media and other otic research. previously, only incomplete information was available regarding the indigenous bacterial flora of the lower intestinal tracts of these coprophagic animals. using the strict anaerobic methodology of the virginia polytechnic institute anaerobe laboratory, we studied the predominant bacterial flora of the cecum and feca ...19883389814
presentation and management of neck abscess: a retrospective analysis.a retrospective analysis of deep neck abscesses managed at the los angeles county-usc medical center was performed. fifty-one patients met the criteria and were reviewed as to presentation, etiology, location, and microbiology. hemolytic streptococci and anaerobic species, especially bacteroides and peptostreptococci, were the most common organisms isolated. intravenous drug abuse was the most common etiology, and was an important source of primary carotid space infection. this group was most co ...19883398666
induction of chronic arthritis in rats by cell wall fragments of anaerobic coccoid rods isolated from the faecal flora of patients with crohn's disease.crohn's disease and ulcerative colitis are accompanied by seronegative arthritis in about one fifth of the cases. in the present study, cell wall fragments from major residents such as eubacterium, coprococcus and peptostreptococcus species, isolated from the faecal flora of patients with crohn's disease, were tested for properties to induce chronic arthritis in lewis rats. cell wall fragments from eubacterium contortum strains me44 and me47 were found to induce chronic arthritis; peptostreptoco ...19883410168
efficacy and safety of single-dose ceftizoxime vs. multiple-dose cefoxitin in preventing infection after vaginal hysterectomy.the efficacy, safety and effect of a single, 1-g dose of ceftizoxime on the return of vaginal flora were compared to those of the standard regimen of three 2-g doses of cefoxitin for prophylaxis in 99 randomized women undergoing vaginal hysterectomy. thirty-four were studied in dallas and 65 in los angeles. surgical procedures were comparable for all the antibiotic groups, although proportionately more simple hysterectomies were performed in dallas. the patients received a povidone-iodine vagina ...19883422693
protein l. a novel bacterial cell wall protein with affinity for ig l chains.a novel ig-binding protein has been isolated from the surface of bacteria belonging to the anaerobic species peptococcus magnus. to solubilize the protein, peptococci were treated with different proteolytic enzymes (papain, pepsin, and trypsin) or with mutanolysin, a bacteriolytic agent known to digest the cell walls of streptococci. papain, trypsin, and mutanolysin all solubilized peptides showing affinity for radiolabeled human igg in western blot analysis. compared with papain and trypsin, mu ...19883125250
characterization of cross-reactive proteins detected by culturette brand rapid latex test for clostridium difficile.clostridium sporogenes, peptostreptococcus anaerobius, and bacteroides asaccharolyticus have been reported to react in the culturette brand rapid latex test (marion scientific, div. marion laboratories, inc., kansas city, mo.) for clostridium difficile. from the results of this study we showed that c. sporogenes and p. anaerobius produce a protein which is very similar biochemically and immunologically to the protein of c. difficile that is detected by the test. thus, the positive latex reaction ...19883128574
increased faecal glycosidases in patients with crohn's disease.the strictly anaerobic faecal flora of patients with crohn's disease (cd) contains higher numbers of coccoid rods (eubacterium, peptostreptococcus and coprococcus species) and gram-negative rods (bacteroides vulgatus) than the flora of healthy subjects. the abnormal flora of patients with cd might affect levels of glycolytic enzymes and metabolic products and thus play some role in the pathogenesis of cd. therefore, the activity of 23 glycosidases that can degrade mucus glycoproteins or plant po ...19873609504
controlled evaluation of modified radiometric blood culture medium supplemented with gelatin for detection of bacteremia and fungemia.although the addition of 1.2% gelatin to broth blood culture media containing sodium polyanetholesulfonate has been shown to enhance detection of certain bacteria, including neisseria meningitidis, n. gonorrhoeae, peptostreptococcus anaerobius, and gardnerella vaginalis, the effect of such supplementation on the detection of other microorganisms causing bacteremia and fungemia is not known. therefore, we studied bactec 6b medium with and without gelatin in 6,833 paired comparisons to examine the ...19873624437
proline requirement for glucose utilization by peptostreptococcus anaerobius atcc 27337.resting cells of peptostreptococcus anaerobius maintained under anaerobic conditions were unable to metabolize either glucose or alanine. the addition of proline to the appropriate suspension, however, resulted in the immediate utilization of both compounds. fermentation of alanine by the cells required that stoichiometric concentrations of proline be present in the medium; and during the oxidation of alanine, proline was simultaneously reduced to the ring cleavage product delta-aminovaleric aci ...19873804441
pathogenicity and therapy of anaerobic bacteria in upper respiratory tract infections. 19873547288
use of intravenous ciprofloxacin in difficult-to-treat infections.intravenous ciprofloxacin was administered to 54 patients who were either critically ill or in whom oral administration was not possible. the 31 males and 23 females ranged in age from 20 to 89 years (mean, 53.2 +/- 17.8 years). patients had "difficult-to-treat" infections, i.e., respiratory infections (15), abscesses (four intraabdominal, three lung, two soft tissue, and one intrahepatic), deep soft tissue infections (10), chronic post-traumatic osteomyelitis in exacerbation (nine), upper urina ...19873555059
comparative activity of metronidazole and tinidazole against clostridium difficile and peptostreptococcus anaerobius.broth dilution mics of metronidazole against 38 strains of clostridium difficile (0.13 microgram/ml) and 11 strains of peptostreptococcus anaerobius (0.11 microgram/ml) were lower than those of tinidazole (0.16 and 0.17 microgram/ml, respectively). as molar concentrations the nitroimidazoles were equally active. the mics of the two drugs correlated positively, and the relative activity of tinidazole increased with decreasing susceptibility of both species.19873566248
viability of lyophilized anaerobes in two media.viability of 15 species of anaerobes was followed after freeze-drying and storage for 1 year. organisms maintained in a 12% sucrose concentration in chopped meat carbohydrate broth survived longer and maintained higher viability counts than those organisms in double-strength skim milk.19873568744
heparinase production by anaerobic bacteria.the production of heparinase by a wide range of anaerobic bacteria isolated from clinical specimens was investigated. none of the 29 strains of bacteroides fragilis produced heparinase. of 62 other bacteroides tested, only two of four strains of b ovatus, two of three strains of b thetaiotaomicron, and two of four strains of b uniformis were heparinase producers. none of the 48 strains of fusobacteria or seven strains of veillonella produced heparinase. the anaerobic cocci (19 peptococci and sev ...19873584480
rapid identification of obligately anaerobic gram-positive cocci using high-performance liquid chromatography.high-performance liquid chromatography was evaluated as a rapid means of identifying obligately anaerobic gram-positive cocci of medical interest. isolates were inoculated into a defined chemical medium consisting primarily of amino acids and were incubated aerobically for 1 h at 35 degrees c. after removal of organisms, the supernatant fluids were derivatized for 1 min at room temperature by the addition of o-phthalaldehyde. the total time required to run a chromatogram was approximately 50 min ...19873597760
effect of streptococcus parvulus and peptostreptococcus magnus on cytotoxin levels of clostridium difficile in anaerobic continuous flow culture.an anaerobic continuous flow (cf) culture method was used in order to study the effect of peptostreptococcus magnus and streptococcus parvulus, anaerobic gram-positive cocci which are members of intestinal bacterial flora, on growth and cytotoxin-activity of clostridium difficile. the growth- and the cytotoxin activity-patterns of c. difficile in an established cf culture of p. magnus were similar to those of c. difficile alone. on the other hand, in the mixed culture system of c. difficile and ...19873431487
bacteria in bile of patients with bile duct inflammation.bile samples taken intraoperatively from 100 patients with three different bile system diseases were subjected to bacteriological analysis. statistically significant differences between the types of aerobic and anaerobic bacteria present in the bile were found.19873436318
[studies on antimicrobial concentrations of flomoxef in serum, pelvic dead space exudate, and pelvic organs/tissues].to women undergoing radical and total hysterectomy, flomoxef (fmox, 6315-s) in a dose of 2 g was administered by intravenous drip infusion over 1 hour and drug concentrations in serum and pelvic dead space exudate as well as pelvic organs/tissues were determined over time. the following results were obtained: 1. serum concentrations of fmox after intravenous infusion showed the peak value of 92.86 +/- 17.05 micrograms/ml at the end of infusion and then gradually decreased to 29.00 +/- 10.49 micr ...19873444023
anaerobic bacteraemia. 19873449455
severe infections of the head and neck resulting from gas-forming organisms: report of case.a case of severe infection of the head and neck associated with gas-forming organisms in a patient with fractures of the mandible is presented. selected aspects of antibiotic therapy are discussed.19873468169
comparative serum bactericidal activity against test anaerobes in volunteers receiving imipenem, clindamycin, latamoxef and metronidazole.ten healthy volunteers received on separate days the following regimens: imipenem 500 mg, clindamycin 600 mg, latamoxef 1 g, and metronidazole 500 mg. the antibiotics were given intravenously as an infusion over 15 min. blood samples were obtained before and 30 min, 1 and 6 h after the start of the infusion. serum bacteriostatic and bactericidal activities were measured against the following strains of strict anaerobes: two strains of bacteroides fragilis, one strain each of b. vulgatus, b. thet ...19873471750
effects of extracellular products of a presumed gingival pathogen, the bacterium peptostreptococcus 84h14s, on cultured human fibroblasts and hela cells.culture fluids from this microorganism, fibroblasts and hela cells contained potent factors which inhibited thymidine uptake in hela cells. the outcome of heating the culture fluid and fractionating it by gel filtration suggested that this was not due to bacterial hyaluronidase activity. purified peptostreptococcal hyaluronidase and several commercially-available mucopolysaccharidases also did not inhibit thymidine uptake.19873478031
[peptostreptococcus sepsis complicating third molar pericoronitis]. 19873483065
the intraocular environment and experimental anaerobic bacterial endophthalmitis.anaerobic bacteria are prevalent in conjunctival flora but have not been adequately investigated as possible causes of endophthalmitis. the mean oxidation-reduction potential (eh) of the rabbit vitreous was found to be +25.1 mv, well within the limiting eh value of many anaerobes. there was an oxygen pressure gradient in the vitreous ranging from 2.1 mm hg immediately posterior to the lens to approximately 20 mm hg adjacent to the medullary ray. endophthalmitis was produced with pure cultures of ...19873499883
microbiological studies of tracheostomy site wounds.specimens were obtained from 25 patients who developed tracheostomy site wound infections. aerobic bacteria only were isolated in 4 (16%) instances, anaerobic bacteria only in 2 (8%), and mixed aerobic and anaerobic isolates were recovered in 19 (76%). a total of 145 isolates (72 aerobes and 73 anaerobes) were recovered, an average of 5.8 isolates per specimen (2.9 aerobes and 2.9 anaerobes). the most frequently recovered isolates were peptostreptococcus sp., bacteroides sp., alpha-hemolytic str ...19873502089
intraventricular haemorrhage complicating a brain abscess.intraventricular haemorrhage occurred in a patient with a parietal rim-enhancing mass on computed tomographic scan. at operation a brain abscess was identified and removed. peptostreptococcus and fusobacterium were isolated, possibly of dental origin. the possible sources of this intracranial bleeding are discussed. a neoplasm should not always be considered in the case of a cerebral ring-enhancing mass complicated with intracranial bleeding; in selected cases, brain abscess should be excluded t ...19873502181
[nonclostridial obligate anaerobes--the main causative agents of lung abscesses].the use of the anaerobic technique in bacteriological investigations has made it possible to establish the leading role of asporogenous anaerobes in the etiological structure of the causative agents of pulmonary abscesses. asporogenous anaerobes have been isolated from 93% of all examined patients, and in 54.4% of such patients these microorganisms have proved to be an independent etiological factor. among asporogenous anaerobes, the main causative agents of pulmonary abscesses have been found t ...19873296575
[revision of the taxonomy of the genera peptococcus and peptostreptococcus and a study on their identification system]. 19873302346
[activity of fusidic acid on strictly anaerobic bacteria].fusidic acid is a well known antimicrobial agent due to its narrow spectrum of activity against gram positive bacteria and especially staphylococci. therefore, it is after used preventively against bacterial infection in traumatology, but the susceptibility of anaerobic bacteria is not well known. we have studied, the in vitro activity of sodium fusidate against 147 strains of anaerobic bacteria. this antibiotic has a moderate activity against bacteroides, more significant against clostridium, p ...19873302863
the growth of oral bacteria on saliva.the present experiments were aimed at studying the degradation of salivary glycoproteins by the oral microflora. to this end, s. sanguis i strain ny476 and s. sanguis ii (s. mitior) strain ny581 were grown continuously in human-whole saliva. under these conditions, the strains produced a variety of cell-associated hydrolytic activities, including glycosidases, exo- and endopeptidases, and esterases. s. sanguis ii generally exhibited higher levels of enzyme activity than did s. sanguis i, in part ...19873305628
direct and indirect pathogenicity of anaerobic bacteria in respiratory tract infections in children.bacteroides sp (bacteroides melaninogenicus, bacteroides oralis, and bacteroides fragilis), peptostreptococci, and fusobacteria are important pathogens in respiratory tract infections (rti). these organisms are often recovered mixed with other aerobic, facultative, and anaerobic bacteria. evidence supporting their virulence is provided by studies showing their synergistic potentials in mixed infections and their increased virulence when encapsulated. a recent increase in numbers of beta-lactamas ...19873318301
omental pedicle graft in the management of infected ascending aortic prostheses.two patients had mediastinal infections with chronic draining sinus tracts that involved a vascular prosthesis in the ascending aorta. in 1 patient, a false mycotic aneurysm developed and in the other, a beginning rupture of the proximal suture line. in both patients, the infection was cured by replacing the infected aortic prosthesis combined with wrapping the new prosthesis with a pedicled omental graft. an omental graft was used to protect the vascular prosthesis and minimize the risk of recu ...19873318738
[clinical studies on cefuzonam in obstetrics and gynecological infections].to evaluate the usefulness and the safety of cefuzonam (czon, l-105) against obstetric and gynecological infections, we conducted clinical trials and obtained the results summarized as follows. 1. thirteen patients with obstetric and gynecological infections (intrauterine infection 2 cases, adnexitis 7, 1 case each of abscess of adnexa uteri, abscess of the vaginal wall, pyelonephritis, and mammitis) were treated with czon. the czon was administered by intravenous injection or intravenous drip i ...19873669283
identification of anaerobic bacteria using high-performance liquid chromatography.20 species of the genera clostridium, bacteroides, fusobacterium and the type strains of peptostreptococcus anaerobius, peptococcus asaccharolyticus, veillonella parvula and propionibacterium acnes were examined for the production of volatile (vfa) and nonvolatile (nvfa) short-chain fatty acids using high-performance liquid chromatography (hplc) with a column for organic acids (aminex hpx-87h). 10 min are needed for sample preparation and the vfa and nvfa were detected simultaneously in a single ...19873673341
interactions of ciprofloxacin with clindamycin, metronidazole, cefoxitin, cefotaxime, and mezlocillin against gram-positive and gram-negative anaerobic bacteria.a total of 598 clinical isolates of anaerobic bacteria were tested against ciprofloxacin by the agar dilution technique with 10(5) cfu on wilkins-chalgren medium. selected strains representative of the six major genera of anaerobes relatively resistant to the quinolones were tested for interactions with ciprofloxacin in combination with clindamycin, metronidazole, cefoxitin, cefotaxime, or mezlocillin by using a checkerboard agar dilution technique. cefotaxime-ciprofloxacin and clindamycin-cipro ...19873674848
enrichment of subgingival microflora on human serum leading to accumulation of bacteroides species, peptostreptococci and fusobacteria.this study was undertaken to identify ecological factors that favour opportunistic pathogenic species in the subgingival microflora. in a first approach, human serum as a substitute for gingival exudate, was used for batch-wise enrichment of subgingival plaque. the microflora resulting after 5-6 enrichment steps consisted of black-pigmented and non-black-pigmented bacteroides species, peptostreptococcus micros and fusobacterium nucleatum as the main organisms. it is noted that the same group of ...19873674857
the in-vitro activity of doxycycline and minocycline against anaerobic bacteria.the likelihood of bacterial resistance now prevents the use of oxytetracycline in the empirical therapy of anaerobic infections. this study investigates the in-vitro activity of two semi-synthetic derivatives, doxycycline and minocycline, against a range of anaerobic bacteria. mics for each antibiotic were determined by an agar incorporation technique. doxycycline and minocycline were four to eight times more active against the majority of strains than oxytetracycline. with the exception of bact ...19873680075
in vitro activity of ampicillin plus sulbactam against anaerobes compared to ampicillin and cefoxitin.the antimicrobial susceptibility of 195 recent clinical isolates of anaerobic bacteria was studied to ampicillin alone, ampicillin + 1 mg/l sulbactam, ampicillin + 5 mg/l sulbactam, and cefoxitin by means of agar dilution tests. the ampicillin-sulbactam combinations were the most effective drugs against species of the bacteroides fragilis group, the mic90 of ampicillin + 5 mg/l sulbactam for b. fragilis being less than 1 mg/l, compared to 256 mg/l of ampicillin, 4 mg/l of ampicillin + 1 mg/l sul ...19873692611
microbiology of retropharyngeal abscesses in children.aspiration of retropharyngeal abscesses was performed in 14 children. cultures were taken from aspirates for aerobic and anaerobic bacteria, and all yielded bacterial growth. anaerobes were isolated in all patients; they were the only organisms isolated in two patients (14%) and were mixed with aerobes in 12 patients (86%). there were 78 anaerobic isolates (5.6 per specimen). the predominant anaerobes were bacteroides species, peptostreptococcus species, and fusobacterium species. there were 26 ...19873812388
bacteraemia and seeding of capsulate bacteroides spp. and anaerobic cocci.the effect of capsulation on the ability of bacteroides fragilis, b. asaccharolyticus and anaerobic gram-positive cocci to induce bacteraemia and seeding to various organs was investigated. the test species were injected into mice subcutaneously alone, or mixed with other aerobic or facultative organisms. capsulate anaerobes were isolated more frequently from the blood, spleen, liver, and kidneys of infected animals than were non-capsulate organisms. after injection of single anaerobic strains, ...19873820272
ampicillin/sulbactam versus metronidazole-gentamicin in the treatment of soft tissue pelvic infections.the clinical efficacy and safety of ampicillin/sulbactam versus metronidazole-gentamicin were evaluated in a comparative, randomized, prospective study. forty-four patients were enrolled: 22 received the ampicillin/sulbactam regimen, and 22 received the metronidazole-gentamicin combination. there were 33 cases of severe acute pelvic inflammatory disease, two tuboovarian abscesses, five cases of endomyometritis, and two cases of posthysterectomy pelvic cellulitis. aerobic and anaerobic cultures f ...19873030109
the effect of carbon dioxide on the in vitro activity of erythromycin and ru-28965 against anaerobic bacteria.the in vitro activity of erythromycin and ru-28965 (a novel macrolide antimicrobial with improved pharmacokinetics) was determined against a variety of anaerobic bacteria in anaerobic atmospheres with and without added carbon dioxide. minimum inhibitory concentrations (mic) were determined using an antimicrobial incorporation technique in wilkins-chalgren agar (oxoid, uk) containing saponinlysed horse blood to a final concentration of 10%. the inoculum used was approximately 10(4) colony forming ...19873113907
myonecrosis of the abdominal wall, complicating radical hysterectomy. 19872978389
in vitro activity of vancomycin and teicoplanin against anaerobic bacteria.the in vitro activity of vancomycin and teicoplanin, a new glycopeptide antimicrobial, was determined against a total of 286 anaerobic bacteria including bacteroides fragilis group (100), b. melaninogenicus (21), b. bivius (16), fusobacterium spp. (15), peptococcus spp. (20), peptostreptococcus spp. (21), clostridium perfringens (23), c. difficile (41) and propionibacterium acnes (29). minimum inhibitory concentrations (mic) were determined using an antimicrobial incorporation technique in wilki ...19872962846
effect of high-fiber and high-oil diets on the fecal flora of swine.six pairs of pigs were fed a basal diet, a high-fiber diet, and a diet high in corn oil in different sequences to minimize the carry-over effect of diet. after 2 months on each diet, a fecal specimen from each pig was cultured on nonselective medium in roll tubes. fifty colonies were randomly selected from each fecal sample, and isolates were characterized to identify a representative cross section of the fecal flora. the bacterial composition of the fecal flora differed between basal and high-f ...19872821900
controlled evaluation of trypticase soy broth with and without gelatin and yeast extract in the detection of bacteremia and fungemia.the addition of gelatin to blood culture media has been suggested to prevent the inhibition of neisseria meningitidis, neisseria gonorrhoeae, gardnerella vaginalis, and peptostreptococcus anaerobius that is caused by sodium polyanetholsulfonate. to determine the effect of such supplementation on the overall yield of microorganisms, we compared the yield and speed of detection of clinically important microorganisms from 5422 paired 10-ml samples of blood cultured in trypticase soy broth (tsb) con ...19872831009
comparative activity of ly146032 against anaerobic cocci.fifty strains of anaerobic gram-positive cocci were tested in vitro against benzylpenicillin, teicoplanin, vancomycin and ly146032. the organisms displayed a wide range of susceptibility to penicillin and the minimum inhibitory concentration for 11 of the strains was greater than or equal to 1 mg penicillin/l. the activity of teicoplanin exceeded that of vancomycin by a factor of two. the activity of ly 146032 varied in different culture media and was dramatically potentiated by the addition of ...19872831046
early microbial colonization of permucosal implants in edentulous patients.in edentulous patients, the microbial colonization of permucosal implants of sintered hydroxyapatite was studied. samples were taken from mucosa and dentures before insertion of implants and from supra- and subgingival sites two to 10 weeks after insertion. in total, five patients and 10 implants with clinically healthy peri-implant tissues were studied. the samples were investigated by dark-field microscopy and anaerobic culture. the supragingival plaque of the implants was dominated by gram-po ...198710872401
sulbactam/ampicillin versus metronidazole/gentamicin in the treatment of severe pelvic infections.the clinical efficacy and safety of sulbactam/ampicillin versus metronidazole/gentamicin were compared in 39 patients with severe pelvic infections. 30 patients had severe acute pelvic inflammatory disease with peritonitis, 3 tubo-ovarian abscesses, 4 endomyometritis, and 2 posthysterectomy pelvic cellulitis. aerobic and anaerobic cultures from the sites of infection yielded 259 micro-organisms from 38 patients; an average of 6.8 bacteria per infection (3.9 anaerobes and 2.9 aerobes). the most f ...19863013566
efficacy of sulbactam plus ampicillin in gynecologic infections.the efficacy of sulbactam plus ampicillin in the treatment of various gynecologic infections was evaluated in 24 women (median age, 35 years). ten women had pelvic cellulitis plus vaginal cuff abscess; six, pyeloperitonitis; three, vaginal cuff abscess; three, surgical wound sepsis; one, tubo-ovarian abscess; and one, endometritis. surgical procedures preceding infection included abdominal hysterectomy, ovarian cyst removal, ectopic pregnancy, correction of cystocele, and uterine dilatation and ...19863026007
anaerobic bacteria in dentoalveolar abscesses.the present study was initiated to determine the bacteriology of 40 orofacial abscesses of dental origin in patients who had taken antibiotics for several days. bacteria were isolated from all but 2 specimens. aerobic or facultative anaerobic bacteria were isolated in 21 cases, obligate anaerobic bacteria in 17 cases, whereas in 11 cases, polymicrobial growth was revealed. the average number of bacterial species was 2.1 per specimen. gram positive aerobic micro-organisms predominated, namely, st ...19863088157
long-term management of patients who have had urinary diversions into colon.thirty-four patients with urinary-colonic diversions have been followed up for 13 to 41 years (mean 20.3). the commonest long-term complication was hyperchloraemic acidosis (50%). the most serious was neoplasm at the anastomotic site: benign lesions occurred in three patients and carcinomas in two (15%). staining for sialomucins in colonic biopsies adjacent to the anastomoses was positive in 17 of 19 patients. it has been suggested that this represents a pre-malignant change. analysis of faecal ...19863099879
[bacterial infection agents in hospitalized patients].during six months the pattern of microbial isolates has been analysed from 1492 urinary tract infections, from 1445 wound infections, from 451 bronchial secretions, from 3067 blood cultures, from 116 peritoneal swabs and from 39 cerebrospinal fluids. all the specimens investigated were taken from patients of surgical, neurosurgical, internal wards and two intensive care units. in total, the most frequent isolates were grampositive microorganisms like staphylococus aureus, straphylococcus epiderm ...19863107262
is ciprofloxacin active against clinically important anaerobes?the comparative activity of ciprofloxacin against 272 clinical isolates and reference strains of anaerobes was determined by an agar dilution method. the majority of strains of bacteroides fragilis and clostridia were resistant (mic greater than 2 mg/l). the effect of ph, inoculum size and medium composition was also studied; the activity of ciprofloxacin was decreased at acid ph and by increasing inoculum size but was unaffected by the composition of the test media. ciprofloxacin should not be ...19862941400
[in vitro effect of piperacillin, amoxicillin, cefoxitin and metronidazole against obligate anaerobic bacteria].the minimum inhibitory concentrations (mic) of piperacillin and three other antibiotics (amoxicillin, cefoxitin, metronidazole) against 165 strains of obligate anaerobes isolated in hospitals in 1985 and 1986 were determined by the wilkins-chalgren agar medium dilution method. among the 67 strains of bacteroides fragilis, 98.5% were sensitive to piperacillin (mic less than or equal to 128 mg/l), 100% were sensitive to metronidazole (mic less than or equal to 4 mg/l), 11.9% were resistant to cefo ...19862949268
the significance of clostridial isolates in intra-abdominal sepsis.in order to evaluate the significance of clostridial species in intra-abdominal infections, the bacteriology records of three hospitals were reviewed during a period of five years. included in this report were 41 patients from whom clostridial species were recovered from specimens of free peritoneal fluid, abscess cavities or bile. seven patients died for a mortality rate of 17.1 per cent. most patients had polymicrobial infections of which clostridial organisms were one of the several anaerobes ...19862876527
pathogenicity of clostridium species with other bacteria in mixed infections.the relationship of clostridial isolates with other bacteria in mixed infections was studied by means of a subcutaneous abscess model in mice. we used 26 isolates of seven clostridial species, two bacteroides spp., eight gram-positive facultative or anaerobic cocci and three enteric gram-negative aerobic rods. abscesses were induced by all seven clostridium perfringens and three c. butyricum isolates and by some of the others. selective antimicrobial therapy experiments showed that enteric gram- ...19862878957
[2 cases of pneumonia with empyema caused by anaerobic micro-organisms in childhood]. 19863826924
comparative activity of sch 34343 against anaerobic bacteria.the in vitro activity of sch 34343 was compared with that of imipenem, clindamycin, cefoxitin, and metronidazole against 78 strains of anaerobic bacteria in the presence and absence of blood. wilkins-chalgren agar was used. sch 34343 and imipenem were the most active agents, inhibiting all strains at less than or equal to 8 micrograms/ml. the addition of blood had little effect on the activity of any of the agents. seven strains were unable to grow on wilkins-chalgren agar. even with the additio ...19863698546
[study of concentrations of clindamycin phosphate in bone marrow blood and tissue].clindamycin phosphate (cldm) was administered to 33 surgical cases in orthopedics to treat or prevent infection (600 mg in 15 cases and 1,200 mg in 18 cases by intravenous drip infusion over 60 minutes) and the concentrations of cldm in the bone marrow blood and tissue of the operative field as well as in venous blood were determined with the cup plate method of bioassay by collecting specimens immediately after completion of infusion or 30 or 60 minutes later. the concentration of cldm in bone ...19863702055
immunochemical characterization of the cell surface carbohydrate antigens of peptostreptococcus anaerobius.two carbohydrate antigens were isolated from the cell surface of peptostreptococcus anaerobius. one, extracted from purified cell walls with naoh, contained glucose and phosphorus, with traces of galactosamine and glucosamine. serological activity was detected by a 'dot blot' procedure. the second antigen, extracted from cell membranes with phenol and purified by chromatography on sepharose 6b and an immunoadsorbent column, contained glucose, glycerol phosphate, phosphorus and fatty acids. antig ...19863711862
microbiologic analyses of nasal polyp tissue.nasal polyps from 40 patients were cultured within 2 1/2 hours after surgical removal to determine whether microorganisms were present. the first 20 polyps were cultured for aerobic and anaerobic bacteria, viruses, fungi, mycoplasmas, and mycobacteria. of these 20 polyps, eight were sterile by all tests, one grew cryptococcus albidus, one grew sporobolomyces, one had large numbers of peptostreptococcus micros and propionibacterium acnes, greater than 10(6) colony-forming units per gram (cfu/gm), ...19863722630
[a case of peptostreptococcal meningitis associated with subarachnoid hemorrhage and subdural hematoma].a very rare case of peptostreptococcal meningitis associated with subarachnoid hemorrhage and subdural hematoma was reported. a 25-year-old man was admitted to st. mary's hospital on november 6, 1984 with a few day's history of headache and low grade fever. on admission, he had high grade fever (39.2 degrees c) and tachycardia (110/min). there were no neurological deficits other than neck stiffness and kernig's sign. the cerebrospinal fluid (csf) which was obtained through lumbar puncture showed ...19863741708
[changes in pathogenic microbes in infectious diseases--infections in otorhinolaryngologic diseases]. 19863761585
[the role of intrauterine contraceptive devices in the development of inflammatory processes in the small pelvis].the incidence of pelvic inflammatory disease (pid) attributable to iud use has been increasing, especially after the removal of the dalkon shield from the market, but this relationship has not been settled conclusively. in recent decades pid included a variety of infections, but lately the definition of pid has meant acute ascending infections of the female genital tract. its most common risk factors include promiscuity of iud use, although this can be reduced to one fourth by regular checkups ...19863763205
[meningitis and brain abscess caused by anaerobic bacteria in an infant]. 19863765691
[effect of metronidazole on the course of experimental anaerobic streptococcal pneumonia].the effect of metronidazole in experimental anaerobic pneumonia in guinea pigs infected with peptostreptococci has been studied. in the untreated animals the prolonged pathological process in the lungs is mainly associated with the suppression of the functional state of the thymus-dependent link of the immune system. metronidazole suppresses anaerobic flora, stimulates local immune reaction and arrests the development of inflammatory changes in the lungs. the drug eliminates the unbalance in som ...19863765970
diabetic foot infections. bacteriologic analysis.diabetic patients with foot infections were prospectively evaluated over a two-year period. cultures from reliable specimens avoiding contamination with foot ulcers were obtained in 54 infectious episodes. staphylococcus species, enterococcus species, corynebacterium species, and various species of enterobacteriaceae were commonly isolated. common anaerobic isolates included peptostreptococcus magnus, peptostreptococcus prevotii, and bacteroides species. results of cultures from 94 unreliable sp ...19863767539
peptostreptococcal vertebral osteomyelitis.the clinical course of a patient with vertebral osteomyelitis caused by peptostreptococcus micros is described. the organism was isolated on two occasions from lumbar vertebral biopsies and was visualized in histologic sections of one of the biopsies. the patient had no apparent infections at other body sites. the infection was eradicated after 6 weeks of intravenous clindamycin therapy.19863771752
[studies of the sensitivity of absolute anaerobes to antibiotics and metronidazole (determination of minimal inhibitory concentrations-mic)]. 19863776192
isolation and characterization of a mucin-degrading strain of peptostreptococcus from rat intestinal tract.a mucin-degrading microorganism was isolated from the intestinal tract by serial sectioning from the serosal side of the caecum wall from a conventional rat. the ability of degrading the intestinal water-soluble mucin was present both in vivo after monocontamination of germ-free rats and in vitro, when adding the microbe to mucin medium. the morphology, gram-positive cocci single or in short chains and the very weak biochemical activities allow us to place this strain in the species peptostrepto ...19863788570
short-term failure of iud removal to alter bacterial flora in a patient with chronic anaerobic endometritis.a patient with cytological and clinical evidence of iud-associated anaerobic endometritis was studied with quantitative and qualitative bacteriological techniques. with the exception of elimination of actinomyces israelii from the deep endocervical/endometrial culture, iud removal induced minimal qualitative changes in the bacterial flora of the endocervical/endometrial sample during the 35 days the patient was monitored. the interposition of menstruation did not significantly alter either the q ...19863789249
mutagenic activation of biliary metabolites of benzo(a)pyrene by beta-glucuronidase-positive bacteria in human faeces.human faeces hydrolysed synthetic beta-d-glucuronides of both p-nitrophenol and phenolphthalein. the origin of this activity in faeces was localised in the bacterial pellet fraction after centrifugation. ninety-seven bacterial strains with beta-glucuronidase activity isolated from fresh human faeces were identified as species of bacteroides, peptostreptococcus, fusobacterium, propionibacterium, clostridium, eubacterium and bifidobacterium. they were classified into two groups according to their ...19863795254
[strict anaeroba in the endodontic region]. 19863506338
[sensitivity of anaerobes to 8 antibiotics].we studied the susceptibility of 282 obligate anaerobes to 8 antibiotics. the strains were isolated from clinical specimens taken during 1983 and 1984. minimal inhibitory concentrations (mics) were determined using a microplate method for 245 strains and agar dilution for 33 bacteroides asaccharolyticus strains. tested strains were as follows: 85 bacteroides fragilis, 71 clostridium, 19 fusobacterium nucleatum, 35 peptostreptococcus, 39 bacteroides oralis and bacteroides bivius and 33 bacteroide ...19863534760
pseudofolliculitis barbae with keloids.pseudofolliculitis barbae is a common cutaneous infection occurring in the bearded area of the face of black men. the infection is caused by ingrown hairs that produce an inflammatory foreign body reaction characterized by papules and pustules at the point of hair penetration that may result in permanent scarring, usually in grooved patterns, and occasionally in keloids. the purpose of this article is to describe the successful treatment of a patient with pseudofolliculitis with keloids that was ...19863540094
in vitro activity of flurithromycin, a novel macrolide antibiotic.flurithromycin is an (8,s)-8-fluoroerythromycin isolated from the fermentation broth of streptomyces erythraeus atcc 31772, a blocked mutant of a strain producer of erythromycin. its in vitro antibacterial activity has been determined on recent clinical isolates of respiratory pathogens. the range of mic for streptococcus pneumoniae and streptococcus beta-haemolyticus group a is from 0.0015 to 0.006 microgram/ml, for haemophilus influenzae from 0.012 to 0.4 microgram/ml, for staphylococcus aureu ...19863487389
[in vitro susceptibilities of brl 25000 (clavulanic acid-amoxicillin) against causative organisms in the field of obstetrics and gynecology].the in vitro susceptibilities of various causative organisms recently isolated from patients with genital infections to brl 25000 (a formulation with 2 parts of amoxicillin and 1 part of potassium clavulanate), amoxicillin (ampc), cefaclor (ccl), cephalexin (cex), cefadroxil (cdx) and cefroxadine (cxd) were determined. beta-lactamase-producing strains were detected by the nitrocefin disc method. frequencies of isolation of beta-lactamase producing strains of e. coli, k. pneumoniae and b. fragili ...19863488425
[fundamental and clinical studies of imipenem and imipenem/cilastatin sodium in the field of obstetrics and gynecology].fundamental and clinical studies of imipenem (mk-0787), a new type of carbapenem antibiotic, and mk-0787 combined with cilastatin sodium (mk-0791), a renal dipeptidase inhibitor, were carried out. the results obtained were as follows: mk-0787 500 mg alone or mk-0787 500 mg with mk-0791 500 mg was administered by intravenous drip infusion over 30 minutes. plasma levels of the drug were similar either following the administration of 500 mg of mk-0787 alone or 500 mg of mk-0787 with 500 mg of mk-07 ...19863463787
gastric acidity: an important factor regulating the composition of the bacterial flora in the small intestine.the role of gastric acidity in regulating the bacterium composition in the small intestine was studied. gastric acidity was determined with the kay test. intestinal juice obtained by a probe was examined for the presence and number of microorganisms. parallel with the increase in gastric acidity, the number of bacteria in the jejunal juice generally decreased. their composition also varied: in anacid patients e. coli predominated whereas in those with free hydrochloric acid, streptococcus alpha ...19863601581
increased resistance towards two systemic experimental infections by tetrachlorodecaoxygen anion complex. possible implications of cellular and humoral immunity.the tetrachlorodecaoxygen anion complex (tcdo, oxoferin) given intravenously 1 h after intravenous infection of mice with candida albicans increased the host's resistance gradually in doses up to 3.1 mumol/kg body weight (b.w.). above this level, the stimulatory effect decreased, turning to an inhibition at a dose of 18.6 mumol/kg b.w. tcdo. repeated applications of the optimum single dose had no cumulative effect. in experimental infections with the strictly anaerobic peptostreptococcus interme ...19863566839
analysis of edta-soluble cell surface components of gram-positive anaerobic cocci.the protein content of edta extracts from 76 strains of gram-positive anaerobic cocci was examined using sds-page. strains of peptostreptococcus anaerobius produced almost identical profiles; greater heterogeneity was observed within the species peptococcus magnus, peptococcus prevotii and peptococcus asaccharolyticus, but several strains within each biotype produced similar patterns. serological investigation of these extracts by elisa revealed numerous cross-reactions among the different bioty ...19863806050
[anaerobic bacteria in various infections and their antibiotic susceptibility patterns].in this study we have examined antibiotic susceptibility of anaerobic bacteria isolated from various samples of 75 patients. anaerobic bacteria from 22 (29.3%) patients, and only aerobic bacteria from 29 (38.7%) patients have been isolated; there has been no growth in cultures from 24 (32%) patients. of 75 isolates from 51 patients 38 (50.6%) are anaerobic and 37 (49.4%) are aerobic bacteria. following anaerobic bacteria have been isolated: peptostreptococcus (13), peptococcus (5), microaerophil ...19863657617
arthropathic properties of cell wall polymers from normal flora bacteria.peptidoglycan-polysaccharide (pg-ps) fragments were purified from cell walls of group d streptococci (streptococcus faecium, strains atcc 9790 and f-24) with a protocol which minimizes autolytic activity and tested for ability to induce arthritis in rats. pg-ps fragments from cell walls of other normal flora bacteria (peptostreptococcus productus, and propionibacterium acnes), group a streptococci, and pseudomurein-ps fragments from cell walls of methanobacterium formicicum, were similarly purif ...19863940996
survival of gram positive anaerobic cocci on swabs and their isolation from the mouth and vagina.the survival of gram positive anaerobic cocci on plain cotton wool and albumin coated swabs held in various transport media was investigated. results suggested that in most cases amies', stuart's and vmgii media do not offer any more protection to the bacteria than storing swabs dry in their containers. a technique was developed for the isolation and identification of gram positive anaerobic cocci from the mouth and vagina, incorporating bicozamycin in the medium as a selective agent. few strain ...19863950035
peptococcaceae bacteremia.bacteremia with peptococcaceae is an uncommon clinical manifestation of infection with this family of microorganisms. a 20-month review of 12 patients with bacteremic infections due to anaerobic gram-positive cocci revealed that obstetrical patients during the peripartum period constitute the group at greatest risk for the development of such infections. eight of the 12 patients were young women hospitalized on the obstetrical service. seven patients had postpartum endometritis and one patient h ...19863956135
[hyperbaric oxygen therapy of anaerobic brain abscesses following tonsillectomy].this study describes the clinical course of a 31 year old woman who developed multiple anaerobic brain abscesses six days after tonsillectomy, followed by hemoparesis and dysarthria. in spite of craniotomy, repeated punctures and drainage of pus and high dose local and systemic antibiotics, there was an obvious deterioration in the patient's condition. hyperbaric oxygen therapy was tried as a last resort. the patient improved quickly, and six months after the tonsillectomy seems to be neurologic ...19853980250
mutagenic activation of biliary metabolites of 1-nitropyrene by intestinal microflora.to investigate the modifying role of intestinal microflora in the metabolism of chemical carcinogens in vivo, we subjected bile from fischer rats treated per os with chemical carcinogens and related compounds to a mutagenicity assay in the presence and absence of a cell-free extract from human feces. a mixture of the bile sample and potassium phosphate buffer was incubated in the presence or absence of human cell-free fecal extract and then further incubated with a bacterial suspension of salmon ...19853982438
serum antibody reactive with predominant organisms in the subgingival flora of young adults with generalized severe periodontitis.in the present study we sought to determine whether serum antibody was present against microorganisms which predominate in the subgingival flora of young adults with generalized severe periodontitis (sp). subjects with sp were often seropositive for eubacterium brachy, fusobacterium nucleatum e3c22, and peptostreptococcus micros, whereas subjects with juvenile periodontitis (jp) and subjects with healthy periodontium (hp) were not. both sp and jp subjects were more frequently seropositive for ba ...19853988337
antimicrobial susceptibilities of anaerobic bacteria isolated from female genital tract infections.certain species or subspecies of anaerobic bacteria are isolated with higher frequency from female genital tract infections than from other anatomic sites. to gain susceptibility data more specific to the treatment of these infections, nine antimicrobial agents were tested by an agar dilution technique against 230 anaerobic bacteria isolated solely from obstetric and gynecological infections. these genital isolates were, in general, very susceptible to imipenem (most active, inhibiting all gram- ...19853994347
beta-glucuronidase activities of intestinal bacteria determined both in vitro and in vivo in gnotobiotic rats.the beta-glucuronidase activities of bacterial strains isolated from the rat intestinal tract were studied both in vitro in culture media and in vivo in the intestinal contents of gnotobiotic rats. only 50 of 407 strains tested were found to be positive in vitro. they belonged to the three genera clostridium, peptostreptococcus, and staphylococcus. the in vitro-negative strains were also negative in vivo. the beta-glucuronidase activities of the beta-glucuronidase activities of the positive stra ...19853994372
changes in prevalence and susceptibility of obligate anaerobes in clinical veterinary practice.of 3,133 clinical specimens obtained from domestic animals, 26% contained species of bacteria that were obligate anaerobes. members of the genera bacteroides, fusobacterium, and peptostreptococcus accounted for 77% of the isolates. on the average, 2 species of obligate anaerobes were found in each specimen, usually admixed with facultatively anaerobic bacteria. of those specimens containing an obligate anaerobe, 20% contained one isolate that was resistant to penicillin, ampicillin, and cephalot ...19853997623
anaerobic bacteria in urine before and after prostatic massage of infertile men.in the present study 25 infertile men delivered urine before and after prostatic massage. expressed prostatic secretion (eps) was obtained from 11 men. aerobic and anaerobic bacterial analyses showed that a total of 33 isolates was found in samples of urine voided before massage as compared to 59 isolates after massage of the prostate. there was an increase in the number of anaerobes whereas there was no change in the number of aerobes. the occurrence of eps did not influence the number of aerob ...19854000063
in vitro activity of cefbuperazone, a new cephamycin, against anaerobic bacteria.the 90% mic of cefbuperazone (bmy 25182) was 32 micrograms/ml for bacteroides fragilis and bacteroides spp., 128 micrograms/ml for fusobacterium and clostridium spp., 64 micrograms/ml for eubacterium and peptococcus spp., 8 micrograms/ml for actinomyces spp., and 32 micrograms/ml for peptostreptococcus spp. the level of activity of cefbuperazone was higher against b. fragilis and lower against anaerobic cocci than those of related cephalosporins, i.e., cefoxitin, cefoperazone, cefotaxime, ceftiz ...19854004195
[a case of preputial calculi].a case of a 73-year-old patient with preputial calculi is presented. the patient visited our hospital complaining of consciousness loss. it was impossible to place a catheter because of complete phimosis. palpatory examination suggested the presence of stones in preputial cavity. dorsal incision was made. after pus discharge nine small stones were extirpated. ammonium hydrogen urate (96%) and calcium phosphate (4%) were found in these stones with infrared spectroscopic analysis. bacteriodes ureo ...19854013948
change in the volatile fatty acids content of laboratory stored sterilized and non-sterilized swine wastes.the influence of urine and microbial activity was studied as possible factors leading to the generation of volatile fatty acids in non-sterilized stored swine wastes. urine was found to promote the generation of the volatile acids. the microbial origin of the volatile acids in the wastes was ascertained by the growth of an anaerobic bacterial strain (peptostreptococcus anaerobius). significant generation and transformation of these acids was observed in sterilized swine waste samples stored unde ...19854033458
a sodium ion gradient as energy source for peptostreptococcus asaccharolyticus.the determination of enzymatic activities in cell-free extracts of acidaminococcus fermentans and peptostreptococcus asaccharolyticus led to a refined scheme for the pathway of glutamate fermentation via (r)-2-hydroxyglutarate to acetate and butyrate. from the ratio of these products the amount of atp generated by substrate level phosphorylation was calculated. growth experiments with the organisms including clostridium symbiosum and clostridium tetanomorphum indicated that a sodium gradient con ...19854037980
[non-puerperal mastitis. etiology, clinical aspects and therapy].non-puerperal mastitis was diagnosed in 79 patients (aged 12-77 years) over the years 1974-1984. malignant neoplasm was not present. bacterial infection in the region of the areola was the most frequent finding (40%), followed by abacterial inflammation without involvement of the nipples (29%). the other cases, bacterial or nonbacterial, occurred at different sites. the histological picture or clinical features of an increased secretory activity of the mammary gland (galactorrhoea, mastodynia) i ...19854038482
rapidly cavitating nodules in a young man. 19854042712
analysis of gram-positive anaerobic cocci in oral, fecal and vaginal flora. 19854043069
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