Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| dissemination of multiresistant enterobacter cloacae isolates producing oxa-48 and ctx-m-15 in a spanish hospital. | twenty-one multiresistant enterobacter cloacae isolates producing oxa-48 (n=10), ctx-m-15 (n=7) or both (n=4) β-lactamases were detected in a spanish hospital during a 1-year period (june 2013 to june 2014). the isolates were also resistant to non-β-lactam antimicrobials, further complicating the therapeutic options. genotyping of the isolates identified two major clones (st74 and st66) that caused prolonged outbreaks in different buildings of the hospital as well as some sporadic isolates (st78 ... | 2015 | 26307466 |
| kpc and vim producing enterobacter cloacae strain from a hospital in northeastern venezuela. | an 83-year-old male patient is admitted to the central hospital in cumana, venezuela with severe urinary infection, history of hospitalizaions and prolonged antimicrobial treatments. a strain of enterobacter cloacae was isolated showing resistance to multiple types of antibiotics (only sensitive to gentamicin), with phenotype of serine- and metallo-carbapenemases. both, bla(vim-2) and bla(kpc) genes were detected in the isolate. this is the first report of an enterobacteriaceae species producing ... | 2015 | 26299058 |
| spontaneous bacterial and fungal infections in genetically engineered mice: is escherichia coli an emerging pathogen in laboratory mouse? | the impact of particular microbes on genetically engineered mice depends on the genotype and the environment. infections resulting in clinical disease have an obvious impact on animal welfare and experimentation. in this study, we investigated the bacterial and fungal aetiology of spontaneous clinical disease of infectious origin among the genetically engineered mice from our institution in relation to their genotype. a total of 63 mice belonging to 33 different mice strains, from severe immunod ... | 2015 | 26281439 |
| bloodstream infections due to enterobacteriaceae among neonates in poland--molecular analysis of the isolates. | bloodstream infections (bsis) are associated with a significantly increased risk of fatality. no report has been found about the molecular epidemiology of enterobacteriaceae causing bsi in neonates in poland. the aim of this work was to determine the antibiotic resistance profiles, virulence gene prevalence, the epidemiological and genetic relationships among the isolates from enterobacteriaceae causing bsi in neonates with birth weight < 1501 g. antimicrobial susceptibility testing was performe ... | 2015 | 26638530 |
| recovery of bacteria and fungi from a leg wound. | acute and chronic wound infections can both be encountered in the deployed setting. these wounds are often contaminated by bacteria and fungi derived from the external environment. in this article, we present the case of a wound infection simultaneously colonized by enterobacter cloacae (a bacterial pathogen) and trichosporon asahii (an unusual fungal pathogen). we describe the examination and treatment of the patient and review the distinguishing characteristics of each organism. | 2015 | 26630106 |
| molecular characteristics of extended spectrum β-lactamase and carbapenemase genes carried by carbapenem-resistant enterobacter cloacae in a chinese university hospital. | in recent years, the increase in the prevalence of carbapenem-resistant enterobacter cloacae (crec) has been significant and has become a global problem. the aim of this study is to characterize crec extended spectrum β-lactamases (esbls) and crec carbapenemase genes and evaluate the risk factors for crec infections in a university hospital in china. | 2015 | 26775390 |
| evaluation of carbapenem-resistant enterobacteriaceae in an italian setting: report from the trench. | the spread of carbapenem resistant enterobacteriaceae (cre) has recently become a matter of concern in public health, mainly due to the wide distribution of carbapenemase genes. italy is a country considered endemic for the spread of blakpc klebsiella pneumoniae (kp). the aim of this study was to depict the epidemiological trend of cre in one italian hospital over a long period (3 years surveillance, from may 2011 to april 2014). based on defined mic cut-off for specific carbapenems, 164 strains ... | 2015 | 25444940 |
| complete genome sequence of enterobacter cloacae ggt036: a furfural tolerant soil bacterium. | enterobacter cloacae is a facultative anaerobic bacterium to be an important cause of nosocomial infection. however, the isolated e. cloacae ggt036 showed higher furfural-tolerant cellular growth, compared to industrial relevant strains such as escherichia coli and corynebacterium glutamicum. here, we report the complete genome sequence of e. cloacae ggt036 isolated from mt. gwanak, seoul, republic of korea. the genomic dna sequence of e. cloacae ggt036 will provide valuable genetic resources fo ... | 2015 | 25444880 |
| extreme furfural tolerance of a soil bacterium enterobacter cloacae ggt036. | detoxification process of cellular inhibitors including furfural is essential for production of bio-based chemicals from lignocellulosic biomass. here we isolated an extreme furfural-tolerant bacterium enterobacter cloacae ggt036 from soil sample collected in mt. gwanak, republic of korea. among isolated bacteria, only e. cloacae ggt036 showed cell growth with 35 mm furfural under aerobic culture. compared to the maximal half inhibitory concentration (ic50) of well-known industrial strains esche ... | 2015 | 25444876 |
| a new oxathiolane from enterobacter cloacae. | enterobacter cloacae is a versatile bacterial species inhabiting a wide variety of niches and is capable of metabolising a wide variety of substances as energy resources. the fermentation culture of this bacterial species has successfully yielded one new compound, rimboxa (1) and three known compounds, i.e. indole-3-carboxaldehyde (2), indole-3-acetic acid (3) and 3,4-di-t-butylaniline (4). rimboxa (1) is shown to possess the 1,2-oxathiolane core structure. 3,4-di-t-butylaniline (4) is isolated ... | 2015 | 25427277 |
| polysaccharides produced by enterobacter cloacae induce apoptosis in cervical cancer cells. | the effects and possible mechanism of polysaccharides produced by enterobacter cloacae (eps) on cervical cancer cells (hela and siha) were investigated in vitro in the current study. the results showed that supplementation of eps inhibited the proliferation of hela and siha cells in a concentration- and time-dependent manner. in addition, the eps also induced the apoptosis of the cervical cancer cells by changing the nuclear morphology and decreasing the mitochondrial membrane potential. further ... | 2015 | 25451749 |
| fecal carriage of extended-spectrum β-lactamase-producing enterobacteriaceae in community setting in casablanca. | the importance of community-acquired infections due to extended-spectrum β-lactamase-producing enterobacteriaceae (esbl-pe) has been increasingly recognized in recent years. this study aimed to determine the prevalence of intestinal carriage of esbl-pe in the community in casablanca, morocco. | 2015 | 25329550 |
| prevalence and characterization of plasmid-mediated quinolone resistance genes in extended-spectrum β-lactamase-producing enterobacteriaceae in a tunisian hospital. | the objective of the study was to assess the prevalence of plasmid-mediated quinolone resistance (pmqr) genes (qnra, qnrb, qnrc, qnrd, qnrs, aac(6')-ib-cr, qepa, and oqxab) in a collection of 120 extended-spectrum β-lactamases (esbls)-producing enterobacteria and to characterize them. overall, pmqr determinants were detected in 72 (60%) isolates (20 escherichia coli, 32 klebsiella pneumoniae, and 20 enterobacter cloacae). pmqr frequencies were as follows: qnr genes (25.8%), oqxab (21.6%), and aa ... | 2015 | 25247633 |
| mlst reveals potentially high-risk international clones of enterobacter cloacae. | to perform the first multinational enterobacter cloacae clonality study, using the mlst scheme newly developed in japan. | 2015 | 25216820 |
| indolent infection in nonunion of the distal femur. | in the treatment of nonunions of the distal femur, infection should be excluded. however, it is difficult to determine whether the nonunion is infected or not with negative history and signs of infection. the purpose of this study was to investigate indolent infection as a cause of presumptive aseptic distal femur nonunion. all presumptive aseptic distal femur nonunions treated from 1998 to 2008 were retrospectively reviewed. any patient with suspected of having an infection clinically was exclu ... | 2015 | 25189289 |
| synthesis and biological evaluation of curcuminoid derivatives. | many curcuminoid derivatives have been reported to have multiple biological activities. the aim of this study was to improve the biological activity of curcuminoids by synthesizing 16 new derivatives which combined cinnamic acids with curcuminoids, and we also analyzed the structure-activity relationship of the new compounds. almost all the new compounds showed encouraging activity, especially compound 7g. it had much better antioxidant activity than curcuminoids and vitamin c (vc), and also had ... | 2015 | 26521852 |
| long-term arsenic monitoring with an enterobacter cloacae microbial fuel cell. | a microbial fuel cell was constructed with biofilms of enterobacter cloacae grown on the anode. bioelectrocatalysis was observed when the biofilm was grown in media containing sucrose as the carbon source and methylene blue as the mediator. the presence of arsenic caused a decrease in bioelectrocatalytic current. biofilm growth in the presence of arsenic resulted in lower power outputs whereas addition of arsenic showed no immediate result in power output due to the short term arsenic resistance ... | 2015 | 25862430 |
| isolation and characterization of endophytic plant growth-promoting bacteria from date palm tree (phoenix dactylifera l.) and their potential role in salinity tolerance. | endophytic bacteria were isolated from date palm (phoenix dactylifera l.) seedling roots, characterized and tested for their ability to help plants grow under saline conditions. molecular characterization showed that the majority of these strains belonged to the genera bacillus and enterobacter and had different degrees of resistance to various antibiotics. some of these strains were able to produce the enzyme 1-aminocyclopropane-1-carboxylic acid (acc) deaminase and the plant growth regulatory ... | 2015 | 25860542 |
| bioaugmentation of biogas production by a hydrogen-producing bacterium. | the rate-limiting nature of the hydrogen concentration prevailing in the anaerobic digester has been recognized, but the associated alterations in the microbial community are unknown. in response to the addition of enterobacter cloacae cells in laboratory anaerobic digesters, the level of biogas production was augmented. terminal restriction fragment length polymorphism (t-rflp) and real-time polymerase chain reaction (real-time pcr) were used to study the survival of mesophilic hydrogen-produci ... | 2015 | 25836037 |
| the three-dimensional structure of vim-31--a metallo-β-lactamase from enterobacter cloacae in its native and oxidized form. | the metallo-β-lactamase vim-31 differs from vim-2 by only two tyr224his and his252arg substitutions. located close to the active site, the tyr224his substitution is also present in vim-1, vim-4, vim-7 and vim-12. the vim-31 variant was reported in 2012 from enterobacter cloacae and kinetically characterized. it exhibits globally lower catalytic efficiencies than vim-2. in the present study, we report the three-dimensional structures of vim-31 in its native (reduced) and oxidized forms. the so-ca ... | 2015 | 25825035 |
| detection and characterization of extended-spectrum beta-lactamase-producing enterobacteriaceae in high-risk patients in an irish tertiary care hospital. | extended-spectrum beta-lactamase-producing enterobacteriaceae (esbl-e) are gram-negative, multi-drug-resistant organisms that are of major clinical significance among immunocompromised patients in high-risk areas in hospital settings. in ireland, the number of esbl-e bloodstream infections is increasing. | 2015 | 25799484 |
| the extract of hypericum ascyron l. induces bacterial cell death through apoptosis pathway. | hypericum ascyron l. (h. ascyron l.) has been used as a traditional medicine for the treatment of wounds, swelling, headache, nausea, stomach ache, abscesses, dysentery and chronic bronchitis. pharmacological studies are necessary to provide a scientific basis to substantiate their traditional use. in this study, antimicrobial effect and its mechanism of the h. ascyron l. extract was investigated. | 2015 | 25796407 |
| medicinal plants used by a mbyá-guarani tribe against infections: activity on kpc-producing isolates and biofilm-forming bacteria. | the traditional use of medicinal plants for treatment of infectious diseases by an indigenous mbyá-guarani tribe from south brazil was assessed by evaluating the antibiotic and antibiofilm activities against relevant bacterial pathogens. aqueous extracts from 10 medicinal plants were prepared according to indigenous mbyá-guarani traditional uses. to evaluate antibiotic (od600) and antibiofilm (crystal violet method) activities, pseudomonas aeruginosa atcc 27853, staphylococcus epidermidis atcc 3 ... | 2015 | 26749812 |
| clonal distribution of multidrug-resistant enterobacter cloacae. | a multilocus sequence typing (mlst) scheme including 7 housekeeping genes was used to evaluate whether the current spread of multidrug-resistant enterobacter cloacae isolates worldwide might be associated to specific successful clones. fifty e. cloacae clinical isolates of worldwide origin, with various β-lactamase content, and recovered at different periods of time were studied. forty-four sequence types were identified, highlighting a high clonal diversity with 3 main lineages. this study reve ... | 2015 | 25680336 |
| pathogenicity of bacteria isolated from gut of spodoptera litura (lepidoptera: noctuidae) and fitness costs of insect associated with consumption of bacteria. | gut microbes contribute to the health of insects and perturbations in the composition or location of gut microbiota can lead to pathological states and host mortality. we explored the culturable bacterial community in the gut of spodoptera litura (fab.) larvae, which is a polyphagous pest. bacterial isolates were identified as microbacterium arborescens (sl6), enterococcus casseliflavus (sl10) and enterobacter cloacae (sl11) by using culture dependent technique based on 16s rrna gene sequencing. ... | 2015 | 25725116 |
| whole genome sequence of enterobacter ludwigii type strain en-119t, isolated from clinical specimens. | enterobacter ludwigii strain en-119(t) is the type strain of e. ludwigii, which belongs to the e. cloacae complex (ecc). this strain was first reported and nominated in 2005 and later been found in many hospitals. in this paper, the whole genome sequencing of this strain was carried out. the total genome size of en-119(t) is 4952,770 bp with 4578 coding sequences, 88 trnas and 10 rrnas. the genome sequence of en-119(t) is the first whole genome sequence of e. ludwigii, which will further our und ... | 2015 | 25724532 |
| structure and genetics of the o-antigen of enterobacter cloacae g3054 containing di-n-acetylpseudaminic acid. | mild acid degradation of the lipopolysaccharide of enterobacter cloacae g3054 resulted in the cleavage of the o-polysaccharide at the linkage of residues of 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid (di-n-acetylpseudaminic acid, pse5ac7ac) in the main chain. the resultant oligosaccharide and an alkali-treated lipopolysaccharide were studied by sugar analysis along with (1)h and (13)c nmr spectroscopy, and the following structure of the branched pentasaccharide o-un ... | 2015 | 25723621 |
| matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof) directly from positive blood culture flasks allows rapid identification of bloodstream infections in immunosuppressed hosts. | in immunosuppressed hosts, rapid identification of microorganisms of bloodstream infections is crucial to ensuring effective antimicrobial therapy. conventional culture requires up to 72 h from sample collection to pathogen identification. | 2015 | 25704776 |
| bacteriological investigation of chronic wounds in a specialized wound healing department: a retrospective analysis of 107 cases. | to investigate the information of chronic wounds, especially in the aspect of microbiological profile and to explore the relationship between the wound culture result and chronic wounds infection, we retrospectively reviewed the medical records of 107 patients with chronic wounds from january 2011 to december 2013. the sociodemographic data, wound-related information, therapeutic type, and wound infection status were extracted. microbial specimens were obtained and processed using standard hospi ... | 2015 | 25700708 |
| assessment of species and antimicrobial resistance among enterobacteriaceae isolated from mallard duck faeces. | mallard ducks have demonstrated to be a likely reservoir for zoonotic e. coli strains; thus, it is possible that these ducks could also act as a reservoir for other enterobacteriaceae members. the present study was initiated to evaluate the species distribution of enterobacteriaceae other than e. coli in 175 fresh faecal samples collected from a population of mallard ducks. sixty-four samples displayed detectable colonies of enterobacteriaceae (excluding e. coli), which resulted in 75 pulsed-fie ... | 2015 | 25697309 |
| enterobacter cloacae pericardial effusion in a frail elderly patient. | we report a case of a frail 82-year-old man with seronegative rheumatoid arthritis and a recent pacemaker insertion, admitted with pulmonary oedema and a symptomatic pericardial effusion. he was treated with diuretics and an urgent pericardiocentesis, a sample from which cultured enterobacter cloacae. a subsequent abdominal ct scan revealed faecal loading, an abnormal anorectal canal and sigmoid colon and a bowel perforation. endoscopy, biopsies and histopathology confirmed a diagnosis of cytome ... | 2015 | 25697299 |
| pancreatic fistulae secondary to trypsinogen activation by pseudomonas aeruginosa infection after pancreatoduodenectomy. | pancreatic fistula after pancreatoduodenectomy (pd) is associated with high mortality and morbidity. trypsinogen activation and bacteria, although hypothesized to be interrelated etiopathogenetically, have not had their relationship and pathogenic mechanisms elucidated. this study investigated bacterial involvement in pancreatic juice activation perioperatively after pd at sites of pancreatic fistula formation. | 2015 | 25678202 |
| studies of house dust mites can now fully embrace the "-omics" era. | 2015 | 25662308 | |
| enhanced biohydrogen production from beverage industrial wastewater using external nitrogen sources and bioaugmentation with facultative anaerobic strains. | in this work biohydrogen generation and its improvement possibilities from beverage industrial wastewater were sought. firstly, mesophilic hydrogen fermentations were conducted in batch vials by applying heat-treated (80°c, 30 min) sludge and liquid (lb-grown) cultures of escherichia coli xl1-blue/enterobacter cloacae dsm 16657 strains for bioaugmentation purposes. the results showed that there was a remarkable increase in hydrogen production capacities when facultative anaerobes were added in t ... | 2015 | 25661265 |
| the dissemination mode of drug-resistant genes in enterobacter cloacae. | enterobacter cloacae (e. cloacae) infection has the highest mortality rate among enterobacter infections. this study aimed to determine the prevalence and the transmission route of the class i integron, qnr genes, and ctx-m esbls genes in clinical isolates and to analyse the association between the prevalence of mdr genes and the antibiotic resistance of e. cloacae. | 2015 | 25657163 |
| acquisition of carbapenem-resistant gram-negative bacilli in intensive care unit: predictors and molecular epidemiology. | we had for aim to determine the risk factors for acquiring carbapenem-intermediate or -resistant gram-negative bacilli (cr-gnb) in an intensive care unit (icu) and to identify the resistance mechanisms involved. | 2015 | 25640914 |
| high prevalence of ctx-m-15 and first report of ctx-m-3, ctx-m-22, ctx-m-28 and plasmid-mediated ampc beta-lactamase producing enterobacteriaceae causing urinary tract infections in bosnia and herzegovina in hospital and community settings. | to investigate molecular epidemiology of extended-spectrum β-lactamase/esbl and plasmid-mediated ampc β-lactamase/pampc producing gram-negative bacteria causing urinary tract infections (utis) in zenica-doboj canton, bosnia and herzegovina, in the period decembar 2009-may 2010. | 2015 | 25638292 |
| carbapenem susceptibility among escherichia coli, klebsiella pneumoniae, and enterobacter cloacae isolates obtained from patients in intensive care units in taiwan in 2005, 2007, and 2009. | to investigate the evolutionary trends in non-susceptibility of carbapenems against the isolates of escherichia coli, klebsiella pneumoniae, and enterobacter cloacae from patients hospitalized in intensive care units (icus) of major teaching hospitals throughout taiwan during 2005-2009, we applied the breakpoints of mics recommended by clinical and laboratory standards institute and european committee on antimicrobial susceptibility testing in 2013. escalations in imipenem mic levels for overall ... | 2015 | 25600841 |
| alveolar and serum concentrations of imipenem in two lung transplant recipients supported with extracorporeal membrane oxygenation. | venovenous extracorporeal membrane oxygenation (ecmo) is increasingly used in patients with respiratory failure who fail conventional treatment. postoperative pneumonia is the most common infection after lung transplantation (40%). imipenem is frequently used for empirical treatment of nosocomial pneumonia in the intensive care unit. nevertheless, few data are available on the impact of ecmo on pharmacokinetics, and no data on imipenem dosing during ecmo. currently, no guidelines exist for antib ... | 2015 | 25572932 |
| emergence of new sequence type oxa-48 carbapenemase-producing enterobacteriaceae in kuwait. | the aim of this study was to investigate the infections due to oxa-48 carbapenemase-producing bacteria in tertiary hospitals in kuwait (september 2011 to april 2013) and to determine the sequence types (sts) of the corresponding isolates. eleven oxa-48 carbapenemase-producing enterobacteriaceae isolates were recovered from patients treated in nine different hospitals in kuwait. susceptibility testing to eighteen antibiotics was done using the e-test. pcr assays were performed for the detection o ... | 2015 | 25551428 |
| probable interspecies transfer of the bla(vim-4) gene between enterobacter cloacae and klebsiella pneumoniae in a single infant patient. | we report the interspecies transfer of the bla(vlm-4) gene in mbl-producing enterobacter cloacae and klebsiella pneumoniae isolates from a newborn patient who had received meropenem therapy. we show evidence that gene bla(vim-4) was transmitted as a part of the class-1 integron on a ca. -90 kb conjugative plasmid. high homology of nucleotide sequence was observed between the integron found in vim-4 producing e. cloacae and k. pneumoniae strains tested and class-1 integrons previously reporteded ... | 2015 | 26999960 |
| genetic analysis of maintenance of pec156, a naturally occurring escherichia coli plasmid that carries genes of the ecoviii restriction-modification system. | in the present study the role of the mechanisms responsible for maintenance of a natural plasmid pec156, that carries genes of the ecoviii restriction-modification system was investigated. analysis of this plasmid's genetic content revealed the presence of genetic determinants suggesting two such mechanisms. the first of them relies on site specific recombination utilizing the xer/cer molecular machinery, while the second involves a restriction-modification system as an addiction module. our ana ... | 2015 | 25500017 |
| metabolic engineering of enterobacter cloacae for high-yield production of enantiopure (2r,3r)-2,3-butanediol from lignocellulose-derived sugars. | biotechnological production of biofuels is restricted by toxicity of the products such as ethanol and butanol. as its low toxicity to microbes, 2,3-butanediol (2,3-bd), a fuel and platform bio-chemical, could be a promising alternative for biofuel production from renewable bioresources. in addition, no bacterial strains have been reported to produce enantiopure 2,3-bd using lignocellulosic hydrolysates. in this study, enterobacter cloacae strain sdm was systematically and metabolically engineere ... | 2015 | 25499652 |
| hypoglycemic and hypolipidemic properties of polysaccharides from enterobacter cloacae z0206 in kkay mice. | the water-soluble polysaccharides (eps) were isolated from culture broth of enterobacter cloacae z0206, and the hypoglycemic and hypolipidemic effects of eps were investigated. in this study, kkay mice were gavaged once daily with either distilled water or eps (200 mg/kg body weight) for 6 weeks. results showed that eps possessed significant hypoglycemic effects. improved oral glucose tolerance, reduced serum insulin levels as well as decreased serum triglycerides (tg), cholesterol (tc) and low ... | 2015 | 25498613 |
| prevalence and diversity of extended-spectrum-β-lactamase-producing enterobacteriaceae from marine beach waters. | a total of 1,351 enterobacteriaceae isolates from 144 seawater samples were collected over a four-year period from three public beaches in the eastern adriatic sea in croatia. approximately 35% of the strains were multidrug-resistant. blaesbl genes were detected in 4.2% of the isolated enterobacteriaceae, the main species of which were escherichia coli, enterobacter cloacae and klebsiella pneumoniae. blatem-1+shv-12 was the most dominant genotype, followed by blactx-m-15.raoultella terrigena and ... | 2015 | 25480155 |
| [malignant pertussis and exchange transfusion]. | malignant pertussis is a critical clinical state associated with fatal outcome in 70% of cases. the severity criteria are a lung infection with pulmonary hypertension and hyperleukocytosis usually above 50 g/l. we report the case of a 2.5-month-old girl hospitalized with critical pertussis in a pediatric intensive care unit. she had acute respiratory distress syndrome with pulmonary hypertension complicated by a bacterial secondary infection with enterobacter cloacae managed by high-frequency os ... | 2015 | 25466784 |
| study of bio-degradation and bio-decolourization of azo dye by enterobacter sp. sxcr. | the objective of this study was to evaluate the decolourization potential of textile dyes by a relatively newly identified bacteria species, enterobacter sp. sxcr which was isolated from the petroleum polluted soil samples. the bacterial strain was identified by 16s rrna gene sequence analysis. the effects of operational conditions like initial dye concentration, ph, and temperature were optimized to develop an economically feasible decolourization process. the isolate was able to decolourize su ... | 2015 | 24645479 |
| in vitro activity of colistin sulfate against enterobacteriaceae producing extended-spectrum β-lactamases. | the widespread multidrug-resistant enterobacteriaceae pose a serious therapeutic challenge. colistin and tigecycline are potential antimicrobial agents for treating infections caused by extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae. we evaluated the in-vitro activity of colistin sulfate against 253 esbl producers isolated from patients admitted to a medical center in southern taiwan (escherichia coli, n = 82; klebsiella pneumoniae, n = 102; enterobacter cloacae, n = 34; and s ... | 2015 | 24388585 |
| phytostabilization of moderate copper contaminated soils using co-inoculation of vicia faba with plant growth promoting bacteria. | there is a need to conduct research on the selection of microbial isolates from rhizosphere of plants growing on heavy metal contaminated soils for specific restoration programs. this article suggest a consortium of bacteria combining rhizobium sp. ccnwsx0481, rhizobium leguminosarum bv. viciae, enterobacter cloacae and pseudomonas sp. 2(2010) that was examined for the ability to promote vicia faba.l. growth when cultivated on the vineyard of soil moderately contaminated with copper. data showed ... | 2015 | 24338717 |
| risk factors of community-onset urinary tract infections caused by plasmid-mediated ampc β-lactamase-producing enterobacteriaceae. | the ampc β-lactamase (ampc)-producing enterobacteriaceae emerged worldwide. this study was conducted to determine the risk factors of community-onset urinary tract infections (utis) caused by plasmid-mediated ampc-producing enterobacteriaceae. | 2015 | 24239065 |
| relationship between the distribution of cefepime minimum inhibitory concentrations and detection of extended-spectrum β-lactamase production among clinically important enterobacteriaceae isolates obtained from patients in intensive care units in taiwan: results from the surveillance of multicenter antimicrobial resistance in taiwan (smart) in 2007. | the data on susceptibility of important cephalosporins against four enterobacteriaceae members producing potential extended-spectrum β-lactamase (esbl) collected from taiwanese intensive care units are lacking. | 2015 | 23973410 |
| infective endocarditis due to enterobacter cloacae resistant to third- and fourth-generation cephalosporins. | we report the case of using a long-term combination of meropenem and amikacin to treat infective endocarditis caused by enterobacter cloacae resistant to third- and fourth-generation cephalosporins. multi-drug resistant gram-negative bacilli, such as the e. cloacae in our study, may become possible pathogens of infective endocarditis. our experience with this case indicates that long-term use of a combination of β-lactam and aminoglycosides might represent a suitable management option for future ... | 2015 | 23017689 |
| thrombolytic therapy using urokinase for management of central venous catheter thrombosis. | the management of central venous catheters (cvcs) and catheter thrombosis vary among centers, and the efficacy of the methods of management of catheter thrombosis in cvcs is rarely reported. we investigated the efficacy of bedside thrombolysis with urokinase for the management of catheter thrombosis. | 2014 | 26217634 |
| temporal dynamics of the very premature infant gut dominant microbiota. | the very-preterm infant gut microbiota is increasingly explored due to its probable role in the development of life threatening diseases. results of high-throughput studies validate and renew the interest in approaches with lower resolution such as pcr-temporal temperature gel electrophoresis (ttge) for the follow-up of dominant microbiota dynamics. we report here an extensive longitudinal study of gut colonization in very preterm infants. we explored by 16s rdna-based pcr-ttge a total of 354 st ... | 2014 | 25551282 |
| positive predictive value of true bacteremia according to the number of positive culture sets in adult patients. | performing multiple blood culture sets simultaneously is a standard blood culture methodology, although it is often difficult to distinguish true bacteremia from contamination when only one of several blood culture sets is positive. this study clarified the relationship between the number of positive blood culture sets and clinical significance in patients with positive blood culture. | 2014 | 25901103 |
| eliminating the heart from the curcumin molecule: monocarbonyl curcumin mimics (macs). | curcumin is a natural product with several thousand years of heritage. its traditional asian application to human ailments has been subjected in recent decades to worldwide pharmacological, biochemical and clinical investigations. curcumin's achilles heel lies in its poor aqueous solubility and rapid degradation at ph ~ 7.4. researchers have sought to unlock curcumin's assets by chemical manipulation. one class of molecules under scrutiny are the monocarbonyl analogs of curcumin (macs). a thousa ... | 2014 | 25547726 |
| genome sequences of two copper-resistant escherichia coli strains isolated from copper-fed pigs. | the draft genome sequences of two copper-resistant escherichia coli strains were determined. these had been isolated from copper-fed pigs and contained additional putative operons conferring copper and other metal and metalloid resistances. | 2014 | 25540351 |
| evolving trna(sec) for efficient canonical incorporation of selenocysteine. | bacterial selenocysteine incorporation occurs in response to opal stop codons and is dependent on the presence of a selenocysteine insertion sequence (secis) element, which recruits the selenocysteine specific elongation factor and trna(sec) needed to reassign the uga codon. the secis element is a stem-loop rna structure immediately following the uga codon and forms part of the coding sequence in bacterial selenoproteins. although the site specific incorporation of selenocysteine is of great int ... | 2014 | 25521771 |
| evolving trna(sec) for efficient canonical incorporation of selenocysteine. | bacterial selenocysteine incorporation occurs in response to opal stop codons and is dependent on the presence of a selenocysteine insertion sequence (secis) element, which recruits the selenocysteine specific elongation factor and trna(sec) needed to reassign the uga codon. the secis element is a stem-loop rna structure immediately following the uga codon and forms part of the coding sequence in bacterial selenoproteins. although the site specific incorporation of selenocysteine is of great int ... | 2014 | 25521771 |
| generating and reversing chronic wounds in diabetic mice by manipulating wound redox parameters. | by 2025, more than 500 m people worldwide will suffer from diabetes; 125 m will develop foot ulcer(s) and 20 m will undergo an amputation, creating a major health problem. understanding how these wounds become chronic will provide insights to reverse chronicity. we hypothesized that oxidative stress (os) in wounds is a critical component for generation of chronicity. we used the db/db mouse model of impaired healing and inhibited, at time of injury, two major antioxidant enzymes, catalase and gl ... | 2014 | 25587545 |
| prevalence of community-acquired bacteraemia in guinea-bissau: an observational study. | the burden of bloodstream infections is insufficiently studied in children in africa and many healthcare facilities lack the capacity to identify invasive disease. often studies have been limited to febrile patients or patients admitted to hospital. | 2014 | 25526763 |
| the effect of the timing of antibiotics and surgical treatment on infection rates in open long-bone fractures: a 6-year prospective study after a change in policy. | our current protocol in treating open long-bone fractures includes early administration of intravenous antibiotics and surgery on a scheduled trauma list. this represents a change from a previous protocol where treatment as soon as possible after injury was carried out. this review reports the infection rates in the period 6 years after the start of this protocol. two hundred and twenty open long-bone fractures were reviewed. data collected included time of administration of antibiotics, time to ... | 2014 | 25526857 |
| rapid isolation of gluten-digesting bacteria from human stool and saliva by using gliadin-containing plates. | the number of individuals with gluten intolerance has increased dramatically over the last years. to date, the only therapy for gluten intolerance is the complete avoidance of dietary gluten. to sustain a strictly gluten-free diet, however, is very challenging. therefore, there is need for a non-dietary therapy. any such treatment must appreciate that the immunogenic part of gluten are gliadin peptides which are poorly degraded by the enzymes of the gastrointestinal tract. probiotic therapy and ... | 2014 | 25519429 |
| rapid isolation of gluten-digesting bacteria from human stool and saliva by using gliadin-containing plates. | the number of individuals with gluten intolerance has increased dramatically over the last years. to date, the only therapy for gluten intolerance is the complete avoidance of dietary gluten. to sustain a strictly gluten-free diet, however, is very challenging. therefore, there is need for a non-dietary therapy. any such treatment must appreciate that the immunogenic part of gluten are gliadin peptides which are poorly degraded by the enzymes of the gastrointestinal tract. probiotic therapy and ... | 2014 | 25519429 |
| epidemiological monitoring of nosocomial infections caused by acinetobacter baumannii. | acinetobacter baumannii is a frequent cause of infections in hospitals around the world, which is very difficult to control and treat. it is particularly prevalent in intensive care wards. | 2014 | 25648217 |
| a national survey of the diagnosis and management of suspected ventilator-associated pneumonia. | ventilator-associated pneumonia (vap) affects up to 20% of patients admitted to intensive care units (icu). it is associated with increased morbidity, mortality and healthcare costs. despite published guidelines, variability in diagnosis and management exists, the extent of which remains unclear. we sought to characterise consultant opinions surrounding diagnostic and management practice for vap in the uk. | 2014 | 25553248 |
| variations in o-antigen biosynthesis and o-acetylation associated with altered phage sensitivity in escherichia coli 4s. | the o polysaccharide of the lipopolysaccharide (o antigen) of gram-negative bacteria often serves as a receptor for bacteriophages that can make the phage dependent on a given o-antigen type, thus supporting the concept of the adaptive significance of the o-antigen variability in bacteria. the o-antigen layer also modulates interactions of many bacteriophages with their hosts, limiting the access of the viruses to other cell surface receptors. here we report variations of o-antigen synthesis and ... | 2014 | 25512310 |
| variations in o-antigen biosynthesis and o-acetylation associated with altered phage sensitivity in escherichia coli 4s. | the o polysaccharide of the lipopolysaccharide (o antigen) of gram-negative bacteria often serves as a receptor for bacteriophages that can make the phage dependent on a given o-antigen type, thus supporting the concept of the adaptive significance of the o-antigen variability in bacteria. the o-antigen layer also modulates interactions of many bacteriophages with their hosts, limiting the access of the viruses to other cell surface receptors. here we report variations of o-antigen synthesis and ... | 2014 | 25512310 |
| a retrospective study of risk and prognostic factors in relation to lower respiratory tract infection in elderly lung cancer patients. | lung cancer (lca) is one of the most common and deadly malignancies in elderly patients. during the course of the disease, these patients frequently present with lower respiratory tract infection. therefore, this study aims to investigate the clinical features of lower respiratory tract infection in elderly lca patients and evaluate the impact on overall survival rate. clinical and laboratory data were analyzed retrospectively for a total of 1936 patients that were over 60-years-old. patients we ... | 2014 | 25628950 |
| a retrospective study of risk and prognostic factors in relation to lower respiratory tract infection in elderly lung cancer patients. | lung cancer (lca) is one of the most common and deadly malignancies in elderly patients. during the course of the disease, these patients frequently present with lower respiratory tract infection. therefore, this study aims to investigate the clinical features of lower respiratory tract infection in elderly lca patients and evaluate the impact on overall survival rate. clinical and laboratory data were analyzed retrospectively for a total of 1936 patients that were over 60-years-old. patients we ... | 2014 | 25628950 |
| first emergence of acrab and oqxab mediated tigecycline resistance in clinical isolates of klebsiella pneumoniae pre-dating the use of tigecycline in a chinese hospital. | tigecycline is one of the few therapeutic options for treating infections caused by some multi-drug resistant pathogens, such as klebsiella pneumoniae. however, tigecycline-resistant k. pneumoniae has been discovered recently in china. from 2009 to 2013, nine tigecycline-resistant k. pneumoniae isolates were identified in our hospital. six of nine strains were identified before using tigecycline. to investigate the efflux-mediated resistance mechanisms of k. pneumoniae, the expression of efflux ... | 2014 | 25503276 |
| draft genome sequence of enterobacter cloacae strain s611. | we report draft genomes of enterobacter cloacae strain s611, an endophytic bacterium isolated from surface-sterilized germinating wheat seeds. we present the assembly and annotation of its genome, which may provide insights into the metabolic pathways involved in adaptation. | 2014 | 25502660 |
| kinetic and structural requirements for carbapenemase activity in ges-type β-lactamases. | carbapenems are the last resort antibiotics for treatment of life-threatening infections. the ges β-lactamases are important contributors to carbapenem resistance in clinical bacterial pathogens. a single amino acid difference at position 170 of the ges-1, ges-2, and ges-5 enzymes is responsible for the expansion of their substrate profile to include carbapenem antibiotics. this highlights the increasing need to understand the mechanisms by which the ges β-lactamases function to aid in developme ... | 2014 | 25485972 |
| kinetic and structural requirements for carbapenemase activity in ges-type β-lactamases. | carbapenems are the last resort antibiotics for treatment of life-threatening infections. the ges β-lactamases are important contributors to carbapenem resistance in clinical bacterial pathogens. a single amino acid difference at position 170 of the ges-1, ges-2, and ges-5 enzymes is responsible for the expansion of their substrate profile to include carbapenem antibiotics. this highlights the increasing need to understand the mechanisms by which the ges β-lactamases function to aid in developme ... | 2014 | 25485972 |
| evaluation of the intron vre vana/vanb real-time pcr assay for detection of vancomycin-resistant enterococci. | recently, the intron vre vana/vanb real-time pcr (intron; intron biotechnology, korea) assay, a multiplex real-time pcr method, was introduced. in this prospective study, we compared the intron assay with the seeplex vre ace detection kit (seeplex; seegene, korea), a conventional multiplex pcr assay. | 2014 | 25553284 |
| evaluation of the intron vre vana/vanb real-time pcr assay for detection of vancomycin-resistant enterococci. | recently, the intron vre vana/vanb real-time pcr (intron; intron biotechnology, korea) assay, a multiplex real-time pcr method, was introduced. in this prospective study, we compared the intron assay with the seeplex vre ace detection kit (seeplex; seegene, korea), a conventional multiplex pcr assay. | 2014 | 25553284 |
| production, characterization, and flocculation mechanism of cation independent, ph tolerant, and thermally stable bioflocculant from enterobacter sp. eth-2. | synthetic high polymer flocculants, frequently utilized for flocculating efficiency and low cost, recently have been discovered as producing increased risk to human health and the environment. development of a more efficient and environmentally sound alternative flocculant agent is investigated in this paper. bioflocculants are produced by microorganisms and may exhibit a high rate of flocculation activity. the bioflocculant eth-2, with high flocculating activity (2849 mg kaolin particle/mg eth- ... | 2014 | 25485629 |
| unusual association of ndm-1 with kpc-2 and arma among brazilian enterobacteriaceae isolates. | we report the microbiological characterization of four new delhi metallo-β-lactamase-1 (blandm-1)-producing enterobacteriaceae isolated in rio de janeiro, brazil. blandm-1 was located on a conjugative plasmid and was associated with klebsiella pneumoniae carbapenemase-2 (blakpc-2) or aminoglycoside-resistance methylase (arma), a 16s rrna methylase not previously reported in brazil, in two distinct strains of enterobacter cloacae. our results suggested that the introduction of blandm-1 in brazil ... | 2014 | 25466163 |
| unusual association of ndm-1 with kpc-2 and arma among brazilian enterobacteriaceae isolates. | we report the microbiological characterization of four new delhi metallo-β-lactamase-1 (blandm-1)-producing enterobacteriaceae isolated in rio de janeiro, brazil. blandm-1 was located on a conjugative plasmid and was associated with klebsiella pneumoniae carbapenemase-2 (blakpc-2) or aminoglycoside-resistance methylase (arma), a 16s rrna methylase not previously reported in brazil, in two distinct strains of enterobacter cloacae. our results suggested that the introduction of blandm-1 in brazil ... | 2014 | 25466163 |
| evaluation of real-time pcr and pyrosequencing for screening incubating blood culture bottles from adults with suspected bloodstream infection. | several molecular platforms can identify bacteria associated with bloodstream infections but require positive culture bottles as starting material. here, we describe results of screening 1140 blood cultures at 8h postinoculation, from 918 eligible adults being evaluated for bloodstream infection. dna was extracted and analyzed by 16s and/or 23s rrna real-time pcr/pyrosequencing. compared to culture, pcr/pyrosequencing displayed 90.9% sensitivity, 99.6% specificity, 95.7% positive predictive valu ... | 2014 | 25534615 |
| evaluation of real-time pcr and pyrosequencing for screening incubating blood culture bottles from adults with suspected bloodstream infection. | several molecular platforms can identify bacteria associated with bloodstream infections but require positive culture bottles as starting material. here, we describe results of screening 1140 blood cultures at 8h postinoculation, from 918 eligible adults being evaluated for bloodstream infection. dna was extracted and analyzed by 16s and/or 23s rrna real-time pcr/pyrosequencing. compared to culture, pcr/pyrosequencing displayed 90.9% sensitivity, 99.6% specificity, 95.7% positive predictive valu ... | 2014 | 25534615 |
| uncommon manifestations of listeria monocytogenes infection. | listeria monocytogenes causes gastroenteritis, meningitis and bacteraemia in immunocompromised, pregnant patients, the elderly as well in immunocompetent patients. focal infections with this organism are uncommon, especially in sporadic (non-outbreak) setting, require high index of suspicion and are challenging to diagnose. we present 3 cases of listeria monocytogenes presenting as focal infections to our hospitals, all of which are the first reported cases from australia. | 2014 | 25466213 |
| screening rectal swabs for carbapenemase genes. | in an outbreak setting, we screened 16,296 samples from 3,644 patients by pcr for the presence of blaoxa-48, blavim, blaimp, blandm, and blakpc. the blaoxa-48 gene was found in samples from 43 patients infected with 9 different species of enterobacteriaceae. five patients had pseudomonas aeruginosa isolates containing blavim. the negative predictive value of screening was 100%, and the positive predictive value was 86%. | 2014 | 25320224 |
| evaluation of high-resolution melting curve analysis of ligation-mediated real-time pcr, a rapid method for epidemiological typing of eskape (enterococcus faecium, staphylococcus aureus, klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, and enterobacter species) pathogens. | a single-tube method, ligation-mediated real-time pcr high-resolution melt analysis (lmqpcr hrma), was modified for the rapid typing of enterococcus faecium, staphylococcus aureus, klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, and enterobacter spp. (eskape) pathogens. a 97% agreement (60/62 isolates) was achieved in comparison to pulsed-field gel electrophoresis (pfge) results, which indicates that lmqpcr hrma is a rapid and accurate screening tool for monitoring nosoco ... | 2014 | 25232168 |
| distribution and drug resistance of pathogenic bacteria isolated from cancer hospital in 2013. | to understand distribution and drug resistance of pathogenic bacteria from a specialized cancer hospital in 2013 in order to provide a basis for rational clinical antimicrobial agents. | 2014 | 25561768 |
| does antimicrobial use density at the ward level influence monthly central line-associated bloodstream infection rates? | the aim of this study was to elucidate risk factors, including ward antimicrobial use density (aud), for central line-associated bloodstream infection (clabsi) as defined by the centers for disease control and prevention in a 430-bed community hospital using central venous lines with closed-hub systems. we calculated aud as (total dose)/(defined daily dose × patient days) ×1,000 for a total of 20 drugs, nine wards, and 24 months. into each line day data, we inputed aud and device utilization rat ... | 2014 | 25525373 |
| first description of oxa-48 carbapenemase harbored by escherichia coli and enterobacter cloacae from a single patient in portugal. | 2014 | 25246399 | |
| genome sequencing of an extended series of ndm-producing klebsiella pneumoniae isolates from neonatal infections in a nepali hospital characterizes the extent of community- versus hospital-associated transmission in an endemic setting. | ndm-producing klebsiella pneumoniae strains represent major clinical and infection control challenges, particularly in resource-limited settings with high rates of antimicrobial resistance. determining whether transmission occurs at a gene, plasmid, or bacterial strain level and within hospital and/or the community has implications for monitoring and controlling spread. whole-genome sequencing (wgs) is the highest-resolution typing method available for transmission epidemiology. we sequenced car ... | 2014 | 25267672 |
| abdominal candidiasis is a hidden reservoir of echinocandin resistance. | fks mutant candida isolates were recovered from 24% (6/25) of abdominal candidiasis patients exposed to echinocandin. candida glabrata (29%) and candida albicans (14%) mutants were identified. multidrug-resistant bacteria were recovered from 83% of fks mutant infections. mutations were associated with prolonged echinocandin exposure (p = 0.01), breakthrough infections (p = 0.03), and therapeutic failures despite source control interventions (100%). abdominal candidiasis is a hidden reservoir for ... | 2014 | 25288081 |
| thiouracil-forming bacteria identified and characterized upon porcine in vitro digestion of brassicaceae feed. | in recent years, the frequent detection of the banned thyreostat thiouracil (tu) in livestock urine has been related to endogenous tu formation following digestion of glucosinolate-rich brassicaceae crops. recently, it was demonstrated that, upon in vitro digestion of brassicaceae, fecal bacteria induce tu detection in livestock (porcine livestock > bovines). therefore, the present study was intended to isolate and identify bacteria involved in this intestinal tu formation upon brassicaceae dige ... | 2014 | 25261511 |
| mechanistic links between gut microbial community dynamics, microbial functions and metabolic health. | gut microbes comprise a high density, biologically active community that lies at the interface of an animal with its nutritional environment. consequently their activity profoundly influences many aspects of the physiology and metabolism of the host animal. a range of microbial structural components and metabolites directly interact with host intestinal cells and tissues to influence nutrient uptake and epithelial health. endocrine, neuronal and lymphoid cells in the gut also integrate signals f ... | 2014 | 25469018 |
| srst2: rapid genomic surveillance for public health and hospital microbiology labs. | rapid molecular typing of bacterial pathogens is critical for public health epidemiology, surveillance and infection control, yet routine use of whole genome sequencing (wgs) for these purposes poses significant challenges. here we present srst2, a read mapping-based tool for fast and accurate detection of genes, alleles and multi-locus sequence types (mlst) from wgs data. using >900 genomes from common pathogens, we show srst2 is highly accurate and outperforms assembly-based methods in terms o ... | 2014 | 25422674 |
| a case of imp-4-, oxa-421-, oxa-96-, and carb-2-producing acinetobacter pittii sequence type 119 in australia. | an imp-4-producing acinetobacter pittii strain coproducing oxacillinases was isolated from a leg wound of a 67-year-old female patient. identification to the species level by rpob and gyrb sequencing and multiplex-pcr-based analysis revealed that the isolate was a. pittii. whole-genome sequencing of this a. pittii isolate determined the presence of blaoxa-96, blacarb-2, and a novel blaoxa-421 gene. the position of this novel blaoxa-421 gene was similar to that of blaoxa-51 in a. baumannii, downs ... | 2014 | 25428154 |
| a case of imp-4-, oxa-421-, oxa-96-, and carb-2-producing acinetobacter pittii sequence type 119 in australia. | an imp-4-producing acinetobacter pittii strain coproducing oxacillinases was isolated from a leg wound of a 67-year-old female patient. identification to the species level by rpob and gyrb sequencing and multiplex-pcr-based analysis revealed that the isolate was a. pittii. whole-genome sequencing of this a. pittii isolate determined the presence of blaoxa-96, blacarb-2, and a novel blaoxa-421 gene. the position of this novel blaoxa-421 gene was similar to that of blaoxa-51 in a. baumannii, downs ... | 2014 | 25428154 |
| two-stage hip revision arthroplasty with a hexagonal modular cementless stem in cases of periprosthetic infection. | two-stage revision arthroplasty is today regarded as the gold standard treatment method for deep prosthetic joint infection. the aim of the present study was to evaluate clinical and functional outcomes with the modular universal tumor and revision system (mutars) rs stem in patients undergoing two-stage revisions. | 2014 | 25428415 |
| laparoscopic excision of a renal subcapsular abscess presenting as a subcapsular haematoma. | renal subcapsular abscess is a very rare entity that is defined by a suppurative process localized to a space between the renal capsule and the renal parenchyma. the pathogenesis and aetiology of this entity remain speculative. to our knowledge, only five cases have been reported in the english literature. we describe a 74-year old woman with renal subcapsular abscess treated with laparoscopic removal and do a review of the literature. | 2014 | 25303183 |
| gut microbiota and metabolic syndrome. | gut microbiota exerts a significant role in the pathogenesis of the metabolic syndrome, as confirmed by studies conducted both on humans and animal models. gut microbial composition and functions are strongly influenced by diet. this complex intestinal "superorganism" seems to affect host metabolic balance modulating energy absorption, gut motility, appetite, glucose and lipid metabolism, as well as hepatic fatty storage. an impairment of the fine balance between gut microbes and host's immune s ... | 2014 | 25473159 |
| prevalence of carbapenemases and metallo-β-lactamases in clinical isolates of enterobacter cloacae. | 2014 | 25664278 | |
| first experience of a multicenter external quality assessment of molecular 16s rrna gene detection in bone and joint infections. | the objective of this study was to assess the performance of seven french laboratories for 16s rrna gene detection by real-time pcr in the diagnosis of bone and joint infection (bji) to validate a large multicenter study. external quality control (qc) was required owing to the differences in extraction procedures and the molecular equipment used in the different laboratories. three proficiency sets were organized, including four bacterial dna extracts and four bead mill-pretreated osteoarticular ... | 2014 | 25411177 |
| first experience of a multicenter external quality assessment of molecular 16s rrna gene detection in bone and joint infections. | the objective of this study was to assess the performance of seven french laboratories for 16s rrna gene detection by real-time pcr in the diagnosis of bone and joint infection (bji) to validate a large multicenter study. external quality control (qc) was required owing to the differences in extraction procedures and the molecular equipment used in the different laboratories. three proficiency sets were organized, including four bacterial dna extracts and four bead mill-pretreated osteoarticular ... | 2014 | 25411177 |