Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| mutations in the gyra and parc genes associated with fluoroquinolone resistance in clinical isolates of citrobacter freundii. | we determined partial sequences of the gyra and parc genes of citrobacter freundii type strain, and then examined 38 c. freundii clinical strains isolated from patients with urinary tract infections for the association of alterations in gyra and parc with susceptibility to fluoroquinolones. our results suggest that in c. freundii dna gyrase may be a primary target of quinolones, that an amino acid change at thr-83 or asp-87 in gyra is sufficient to decrease susceptibility to fluoroquinolones, an ... | 1997 | 9311142 |
| effect of vancomycin on intestinal flora of patients who previously received antimicrobial therapy. | to evaluate the ecological disturbances of peroral vancomycin administration following cephalosporin administration, 20 healthy volunteers received cefuroxime axetil tablets (250 mg) perorally twice a day for 1 week, and 10 of these volunteers subsequently received vancomycin capsules (125 mg) perorally four times daily for 7 days. the concentration of vancomycin in feces after 1 week of vancomycin administration was high (mean +/- sd, 520 +/- 197 mg/kg), which correlated with the ecological dis ... | 1997 | 9314469 |
| distribution of a sub-class of bacterial abc polar amino acid transporter and identification of an n-terminal region involved in solute specificity. | a new sub-class of binding protein-dependent transporter with specificity for a broad range of polar amino acids has been identified by sequence comparison, in rhizobium leguminosarum, rhodobacter capsulatus, escherichia coli and pseudomonas fluorescens. southern blotting and pcr analysis has shown that transporters from this new sub-class are widely distributed in gram-negative bacteria, including, in addition to the above, citrobacter freundii, erwinia carotovorum and rhizobium meliloti. abc t ... | 1997 | 9315727 |
| molecular epidemiology of a nosocomial outbreak due to shv-4-producing strains of citrobacter diversus. | over a 6-month period, eight strains of citrobacter diversus (citrobacter koseri) resistant to extended-spectrum cephalosporins and monobactams were isolated from seven colonized and/or infected patients from the same intensive care unit. all strains harbored a single large conjugative plasmid which mediated an extended-spectrum beta-lactamase of the shv-4 type (ceftazidimase phenotype; enzyme pi, 7.8; plasmid dna hybridization with a blashv-specific probe). all strains were characterized by ant ... | 1997 | 9316908 |
| occurrence and detection of extended-spectrum beta-lactamases in members of the family enterobacteriaceae at a veterans medical center: seek and you may find. | a total of 907 consecutive isolates of members of the family enterobacteriaceae recovered during a 20-week period were tested for production of extended-spectrum beta-lactamases (esbls) by the double-disk (dd) potentiation method. of 84 dd-positive isolates, 83 (9.2%) produced esbls based on isoelectric focusing. shv-derived esbls and several tem-derived esbls were present in nine species, including the first isolate of citrobacter koserii and morganella morganii known to harbor an shv-derived e ... | 1997 | 9316913 |
| molecular epidemiology of acquisition of ceftazidime-resistant gram-negative bacilli in a nonoutbreak setting. | we prospectively studied the acquisition of ceftazidime-resistant gram-negative bacilli (caz-rgn) in two surgical intensive care units (sicu) during a nonoutbreak period. surveillance cultures were obtained from patients at the time of admission and serially thereafter. caz-rgn isolates were typed by pulsed-field gel electrophoresis (pfge). three hundred and forty-three patients were enrolled from whom 1,621 baseline and follow-up cultures were obtained. the most common species isolated from pat ... | 1997 | 9316915 |
| citrobacter sedlakii meningitis and brain abscess in a premature infant. | citrobacter sedlakii was isolated from blood and cerebrospinal fluid cultures of a 5-day-old premature infant with sepsis, meningitis, and brain abscess. this newly described organism was difficult to identify due to discrepancies between the vitek and api 20e identification systems. to our knowledge, this is the first report of the isolation of c. sedlakii from cerebrospinal fluid. | 1997 | 9316937 |
| inducible amp c beta-lactamase producing gram-negative bacilli from blood stream infections: frequency, antimicrobial susceptibility, and molecular epidemiology in a national surveillance program (scope). | a surveillance study of nosocomial blood stream infections [surveillance and control of pathogens of epidemiologic importance (scope)] was conducted during a 14-month period in 1995 to 1996 in approximately 50 american medical centers. among the 4725 blood stream infections, the etiologic agent was enterobacter spp. in 230, citrobacter freundii in 24, and serratia marcescens in 65. the vast majority of these isolates (89%) had been sent to the university of iowa including 198 enterobacter spp. ( ... | 1997 | 9327251 |
| mechanism of suppression of piperacillin resistance in enterobacteria by tazobactam. | resistance to piperacillin in several isolates of citrobacter freundii and enterobacter cloacae was investigated and confirmed to occur at a frequency of 10(-7) to 10(-6). development of resistance to piperacillin was significantly suppressed by tazobactam but not by clavulanic acid. to elucidate the mechanism by which resistance suppression occurs, the effect of piperacillin plus tazobactam on the induction of ampc beta-lactamase was analyzed by monitoring the beta-galactosidase activity of an ... | 1997 | 9333044 |
| propanediol utilization genes (pdu) of salmonella typhimurium: three genes for the propanediol dehydratase. | the propanediol utilization (pdu) operon of salmonella typhimurium encodes proteins required for the catabolism of propanediol, including a coenzyme b12-dependent propanediol dehydratase. a clone that expresses propanediol dehydratase activity was isolated from a salmonella genomic library. dna sequence analysis showed that the clone included part of the pduf gene, the pduabcde genes, and a long partial open reading frame (orf1). the clone included 3.9 kbp of pdu dna which had not been previousl ... | 1997 | 9352910 |
| neonatal meningitis and multiple brain abscesses due to citrobacter diversus. | we report a fatal sporadic case of neonatal citrobacter diversus meningitis with rapid clinical progression. at autopsy, multiple brain abscesses and abscesses in the spinal cord had complicated purulent meningitis. septic omphalitis was identified as the most likely portal of entry. | 1997 | 9353837 |
| carbapenem resistance in a clinical isolate of citrobacter freundii. | carbapenem resistance was studied in two sets of citrobacter freundii strains: (i) strain cfr950, resistant to imipenem (mic, 16 microg/ml) and isolated in vivo during imipenem therapy, and strain cfr950-rev, the spontaneous, imipenem-susceptible revertant of cfr950 selected in vitro, and (ii) strains cfr801 and cfr802, two imipenem-resistant mutants selected in vitro from the susceptible clinical isolate cfr800. in all strains, whether they were imipenem-susceptible or -resistant strains, produ ... | 1997 | 9371332 |
| cloning and characterization of an aminoglycoside 6'-n-acetyltransferase gene from citrobacter freundii which confers an altered resistance profile. | a novel gene encoding a 6'-n-aminoglycoside acetyltransferase, aac(6')-in, has been cloned and sequenced from citrobacter freundii 13996-19, a clinical isolate from venezuela. this gene mediates resistance to amikacin, 2'-n-ethylnetilmicin, isepamicin, kanamycin, netilmicin, and tobramycin. the aac(6')-in gene is 573 nucleotides in length and encodes a putative protein of 190 amino acids. aac(6')-in is most closely related to aac(6')-im and aac(6')-ie, demonstrating 64.4% and 62.3% similarity, r ... | 1997 | 9371347 |
| characterization of blatem-3 and blashv-4 beta-lactamase-encoding genes in citrobacter diversus. | 1997 | 9371377 | |
| vertical transmission of a citrobacter infection. | citrobacter species have rarely been described as-etiological factors of intraamniotic infections. citrobacter is not a normal inhabitant of the female genital tract. vertical transmission of citrobacter from mother to fetus has rarely been reported. a 21-year-old primigravida presented to labor and delivery at 40 6/7 weeks' gestation complaining of ruptured membranes, painful uterine contractions, and fever. an intraamniotic infection was diagnosed and antibiotics begun. she was subsequently de ... | 1997 | 9376007 |
| 3-methylaspartate ammonia-lyase as a marker enzyme of the mesaconate pathway for (s)-glutamate fermentation in enterobacteriaceae. | the enzyme 3-methylaspartase (3-methylaspartate ammonia-lyase, ec 4. 3.1.2) was found in the cells of enteric bacteria, especially in the genera citrobacter and morganella, that were grown under anoxic and oxygen-limited conditions. the enzymes were purified to homogeneity from the cell-free extracts of 18 active strains and had similar enzymological properties such as action on columns, specific activity, molecular weight, subunit structure, and n-terminal amino acid sequence similarity. the pr ... | 1997 | 9385136 |
| antibiotic susceptibility of blood culture isolates of enterobacteriaceae from six norwegian hospitals 1991-1992. | from august 1991 to february 1992, each of the six largest hospitals throughout norway collected 84 to 107 consecutive blood culture isolates of enterobacteriaceae, altogether 571 isolates. the distribution of various species and genera at the different hospitals was uniform; escherichia coli being most prevalent (57-67%), followed by klebsiella spp. (12-18%) and proteus mirabilis (7-11%). twenty-one and 4% of e. coli isolates were resistant to ampicillin and cefuroxime, respectively, and 11% of ... | 1997 | 9393556 |
| citrobacter farmeri bacteremia in a child with short-bowel syndrome. | a case of sepsis in a pediatric patient due to the newly described citrobacter species c. farmeri is described. factors predisposing this child to infection included short-bowel syndrome requiring total parenteral nutrition. | 1997 | 9399558 |
| production of escherichia coli group i-like heat-labile enterotoxin by enterobacteriaceae isolated from environmental water. | strains of klebsiella oxytoca (2), citrobacter freundii (2), enterobacter cloacae (1) and escherichia coli (1) isolated from environmental water were identified as heat-labile enterotoxin (lt) producing strains by immunological methods and polymerase chain amplification. a 322 bp amplified fragment was obtained with specific primers ltr and ltl, and hybridized to a digoxigenin-labelled ltb probe only under low stringency conditions, and not with a cholera toxin probe. these results suggest that ... | 1997 | 9418038 |
| comparison of viab regions of vi-positive organisms. | we cloned the vipr genes from salmonella paratyphi c, s. dublin, and citrobacter freundii strains and compared them with the s. typhi sequence to clarify the genetic relationship of the viab regions of vi-positive organisms. viab regions were divided into two groups based on their sequences, the salmonella and c. freundii groups. the vipr coding sequences of the salmonella group were identical. southern blot hybridization results using the full-length viab region as a probe support these finding ... | 1997 | 9418239 |
| in vitro and in vivo antibacterial activities of cs-834, a novel oral carbapenem. | cs-834 is a novel oral carbapenem antibiotic. this compound is an ester-type prodrug of the active metabolite r-95867. the antibacterial activity of r-95867 was tested against 1,323 clinical isolates of 35 species and was compared with those of oral cephems, i.e., cefteram, cefpodoxime, cefdinir, and cefditoren, and that of a parenteral carbapenem, imipenem. r-95867 exhibited a broad spectrum of activity covering both gram-positive and -negative aerobes and anaerobes. its activity was superior t ... | 1997 | 9420035 |
| regulation of whole blood tumor necrosis factor production upon endotoxin stimulation after severe blunt trauma. | trauma has been recognized to be accompanied by alterations of leukocyte functions such as cytokine release. the regulatory principles involved in these changes are still poorly defined. to further characterize leukocyte function after multiple trauma, endotoxin-stimulated tumor necrosis factor (tnf) production of trauma patients' whole blood and a possible regulatory mechanism were studied. | 1997 | 9420099 |
| detection and speciation of bacteria through pcr using universal major cold-shock protein primer oligomers. | the detection of bacteria using pcr is a well-established diagnostic technique. however, conventional pcr requires the use of dna primer oligomers that are specific to the target organism and, as a consequence, a sample can only be tested for the presence of that specific target. a significant advantage would be to probe a sample for the presence of any bacteria, followed by identification. to achieve this it is necessary to identify a dna sequence common to all bacteria. here we demonstrate tha ... | 1997 | 9439003 |
| comparative in vitro activity of meropenem versus other extended-spectrum antimicrobial agents against 2,085 clinical isolates tested in 13 brazilian centers. | meropenem is a parenteral carbapenem antibacterial agent with a very broad spectrum of antibacterial activity. it is the second agent of its class to become available in brazil. the in vitro antibacterial activity of meropenem was compared with imipenem and four other antimicrobial agents in a multicenter study. this study involved 13 clinical microbiology laboratories, 10 of which came from 8 brazilian states. a total of 2,085 clinical isolates consecutively collected between december 1995 and ... | 1997 | 11105151 |
| beta-lactamases: quantity and resistance. | rates of enzyme-mediated catalysis are proportional to enzyme quantity, so increasing the amount of beta-lactamase should increase resistance. kinetic considerations support this argument for both gram-positive and gram-negative bacteria. direct relationships between resistance and enzyme quantity are most obvious with constitutive beta-lactamases, e.g. the tem types from gram-negative bacteria. as the level of tem enzyme rises, so do the mics of substrates and the concentrations of inhibitors r ... | 1997 | 11869238 |
| differentiation and susceptibility of citrobacter isolates from patients in a university hospital. | objective: recently a publication of brenner et al. introduced 11 genetically distinct species within the genus citrobacter. these newly recognized citrobacter species can be classified by means of their biochemical characteristics. the aim of this study was to examine the distribution and susceptibility of citrobacter isolates in our patient population. methods: a total of 126 samples---containing a citrobacter species---was collected from 116 hospitalized patients during a 6-month period. orga ... | 1997 | 11864076 |
| high frequency of antibiotic resistance among gram-negative isolates in intensive care units at 10 swedish hospitals. | objective: to investigate the resistance rates among gram-negative isolates in swedish intensive care units (icus). methods: during 1994-95, members of the swedish study group collected, on clinical indication, 502 consecutive initial isolates of gram-negative bacteria from patients admitted to icus at 10 swedish hospitals and performed minimal inhibitory concentration (mic) determinations with the etest. breakpoints were defined according to the criteria of the swedish reference group for antib ... | 1997 | 11864106 |
| cq-397 and cq-414: antimicrobial activity and spectrum of two fluoroquinolone---cephalosporin, dual-action compounds with carboxamido bonds. | objective: to evaluate the potential spectrum of activity of two novel dual-action compounds with carboxamido bonds (cq-397 and cq-414; laboratorios aranda, san rafael, mexico) against human pathogens. methods: approximately 800 gram-positive and gram-negative aerobic clinical bacteria were tested in vitro using the mueller-hinton broth microdilution method of the national committee of clinical laboratory standards. results: cq-397 (cefamandole+enrofloxacin) and cq-414 (cefamandole+norfloxacin) ... | 1997 | 11864130 |
| cloning of a transaminase gene of citrobacter freundii and the gene dosage effect on l-phenylalanine production through transaminase reaction. | 1997 | 12501345 | |
| antimicrobial activity and durability of a novel antimicrobial-impregnated bladder catheter. | the main objective of this study was to examine the antimicrobial activity and durability of a novel indwelling bladder catheter impregnated with minocycline and rifampin. thirty antimicrobial-impregnated bladder catheters were inserted transurethrally in spinal cord-injured patients and removed, in six groups of five catheters each, at 3, 7, 10, 14, 17 or 21 days. removed catheters had detectable zones of inhibition against two different clinical isolates of each of the 10 tested uropathogens ( ... | 1997 | 18611809 |
| microbially enhanced chemisorption of nickel into biologically synthesized hydrogen uranyl phosphate: a novel system for the removal and recovery of metals from aqueous solutions. | ni(2+) was removed quantitatively from aqueous flows by columns loaded with polycrystalline hydrogen uranyl phosphate (hup) bound to immobilized cells of citrobacter sp. the columns functioned effectively in ni uptake/regeneration cycles; five cycles were completed without significant decrease in the ni-removing capacity of the column. the influence of ph, temperature, and flow rate on the ni-removing capacity of the columns was examined. the composition of the ni/hup cell-bound deposits was con ... | 1997 | 18634098 |
| effect of substrate concentration and nitrate inhibition on product release and heavy metal removal by a citrobacter sp. | a citrobacter sp. accumulates heavy metals as cell-bound metal phosphates, utilizing phosphate released by the enzymatic cleavage of a phosphomonoester substrate. the effect of increased substrate (glycerol 2-phosphate, g2p) concentration on phosphate release and heavy metal accumulation was evaluated using a stirred tank reactor (str) and a plug flow reactor (pfr). a significant improvement in metal removal was achieved with increased substrate concentration using immobilized citrobacter cells ... | 1997 | 18636593 |
| [characterization of antimicrobial properties of cefpirome]. | the results of a multicentre investigation of antibiotic susceptibility in 800 clinical isolates were analyzed. the levels of susceptibility to cefpirome and other antibiotics in gram-negative organisms with inducable production of chromosomal beta-lactamases (enterobacter, serratia, citrobacter and others) were compared and the advantages of cefpirome over other cephalosporins were shown: 89 per cent of the susceptible strains against 58 to 78 per cent. with respect to other microorganisms the ... | 1996 | 9124987 |
| [lipopolysaccharides containing sialic acid]. | sialic acids which are important constituents of animal tissue glycoconjugates are also present in antigens of some bacterial strains. capsular polysaccharides with sialic acid have been extensively studied whereas little is known on lipopolysaccharides which contain sialic acid. the paper presents review of the data concerning structure of bacterial endotoxic lipopolysaccharides. methodological peculiarities were described. specially emphasized were endotoxins of escherichia coli o24, o56, o104 ... | 1996 | 9072762 |
| [fluoroquinolone and aminoglycoside resistance in chromosomal cephalosporinase-overproducing gram-negative bacilli strains with inducible beta-lactamase]. | the increasing prevalence of stable derepressed mutants over-producers of type i chromosomal cephalosporinase in inducible enterobacteriaceae and pseudomonas aeruginosa challenges the adequacy of third generation cephalosporins in the empirical treatment of certain nosocomial infections. we sought to determine the frequency of stable over-producers of type i enzyme and their associated resistance to fluoroquinolones and aminoglycosides. | 1996 | 9044634 |
| [antimicrobial activities of cefetamet against clinical isolates from urinary tract infection]. | in order to evaluate antimicrobial activity of cefetamet (cemt), minimum inhibitory concentrations (mics) of cemt and control drugs were determined against gram-negative rods mainly from complicated urinary tract infections examined in our laboratory from april to september of 1994. the results are summarized as follows; 1. the obtained strains were citrobacter diversus 20, citrobacter freundii 30, enterobacter aerogenes 20, enterobacter cloacae 30, serratia marcescens 30, proteus mirabilis 30, ... | 1996 | 9032594 |
| iron utilization studies in citrobacter species. | seventy-one strains of citrobacter were screened for iron scavenging mechanisms by biologic and chemical assays. essentially all citrobacteria (70/71) were found to elaborate enterobactin-like siderophores by both biologic and chemical assays, however only c. koseri (c. diversus) was found to produce aerobactin. the concentration of ethylenediamine di(o-hydroxyphenylacetic acid) (edda) required to inhibit the growth of individual citrobacter strains by depleting free iron ranged from 250 microgr ... | 1996 | 8998976 |
| antimicrobial effects of oligoamines. | twenty-four oligoamines belonging to six (1-6) structurally different types were tested in vitro for their antibacterial activity against 14 different bacterial species comprising a total of 187 strains. ten compounds were able to inhibit growth of at least one strain at concentrations < or = 10 mumol/l. for three compounds, minimum inhibitory concentrations for some strains were even below 1 mumol/l. clear structure-activity relationships showed that the inhibitory effect depended on the bridge ... | 1996 | 9005815 |
| screening of in vitro antibacterial activity from syzygium guineense (willd) hydrosoluble dry extract. | diarrhoea is one of the most important causes of infant mortality in the world. as modern drugs are expensive or unavailable in developing countries, many people use traditional medicines in africa for the treatment of several diseases. in our study, we investigated the antibacterial activity of syzygium guineense extract in order to assess its activity on some bacterial strains involved in diarrhoeal diseases and to justify its use. the aqueous dry extract was prepared by decoction followed by ... | 1996 | 9008903 |
| re-speciation of the original reference strains of serovars in the citrobacter freundii (bethesda-ballerup group) antigenic scheme of west and edwards. | the antigenic scheme for the bethesda-ballerup group of bacteria established by west and edwards in 1954 has continued to be applied as a serotyping scheme for citrobacter freundii. in 1993, however, the classification of the citrobacter was drastically revised and the species c. freundii redefined by brenner et al. accordingly, to judge the propriety to continuously use a single antigenic scheme for the c. freundii complex, the 90 reference strains listed in the antigenic scheme for c. freundii ... | 1996 | 9013489 |
| [use of semi-solid diasalm medium for isolation of salmonella enteritidis]. | the diagnostic semi-solid salmonella agar (diasalm) was compared with two commercial semi-solid rappaport-vasiliadis media (msrv by oxoid and srva, basis by himedia) using 52 strains of salmonella and 10 strains of interfering gram-negative bacteria. the diagnostic potency for salmonellae was higher in diasalm than in msrv or srva. unlike the rappaport-vasiliadis media, diasalm contains a diagnostic system consisting of lactose, saccharose and bromocresol purple, allowing the differentiation of ... | 1996 | 8966968 |
| [sympathetic reflex dystrophy: an uncommon illness in childhood]. | 1996 | 8967653 | |
| [diagnosis and management of acute haematogenous osteomyelitis in newborns and infants]. | many newborns in poland still suffer from acute hematogenous osteomyelitis. the paper discusses the principles of early diagnosis and treatment. | 1996 | 8975223 |
| [enterobacteria in areas of water along the crimean coast]. | characteristic of coliform bacteria in the coastal sea ecosystems is given. a decrease of escherichia coli indicator value was ascertained and presence of klebsiella sp. and citrobacter freundii was revealed, in the sea areas with high level of anthropogenic load. wide distribution of bacteria of enterobacter agglomerans kind was determined in the sea substrates and their stability to unfavourable influences was shown. | 1996 | 8983520 |
| transmission of citrobacter koseri from mother to infant documented by ribotyping and pulsed-field gel electrophoresis. | we describe a case in which citrobacter koseri (formerly c. diversus) was transmitted from a pregnant mother with chorioamnionitis and bacteremia to her infant who was bacteremic at birth and in apparent septic shock. two highly discriminating molecular methods, ribotyping and pulsed field gel electrophoresis, were used to examine restriction fragment length polymorphisms within the genomic dna of maternal and infant isolates. both techniques identified the maternal and infant isolates as the sa ... | 1996 | 8985657 |
| [antimicrobial activity of cefodizime against clinical isolates]. | in order to evaluate antimicrobial activity of cefodizime (cdzm), minimum inhibitory concentrations (mics) of cdzm and control drugs were determined against clinical isolates collected from nation-wide medical institutions and in our laboratory from september to december of 1992 and from september to december of 1995. the results are summarized as follows: 1. bacterial species with no or few strains resistant to cdzm included streptococcus pyogenes, haemophilus influenzae, citrobacter koseri, pr ... | 1996 | 8986558 |
| variation in salmonella core lipopolysaccharide as detected by the monoclonal antibody m105. | the lipopolysaccharide antigenicity of 22 salmonella strains (representing nine serogroups) and four non-salmonellae enterobacteriaceae to the salmonella genus specific monoclonal antibody m105 was analysed. the monoclonal antibody m105 reacted with all 22 salmonella strains. probing sds-page separated lps molecules with mab m105 revealed that the antibody reacted with the core region of all salmonella serovars. however, no reaction was obtained to the long-chain lps of serovars o (salm. adelaid ... | 1996 | 8987449 |
| [lytic lesion of the distal phalanx of a patient with systemic circumscribed sclerosis]. | 1996 | 8991443 | |
| isolation, identification, and transcriptional specificity of the heat shock sigma factor sigma32 from caulobacter crescentus. | we report the identification of the caulobacter crescentus heat shock factor sigma32 as a 34-kda protein that copurifies with the rna polymerase holoenzyme. the n-terminal amino acid sequence of this protein was determined and used to design a degenerate oligonucleotide as a probe to identify the corresponding gene, rpoh, which encodes a predicted protein with a molecular mass of 33,659 da. the amino acid sequence of this protein is similar to those of known bacterial heat shock sigma factors of ... | 1996 | 8606189 |
| the detection of verocytotoxins in bacterial cultures from human diarrhoeal samples with monoclonal antibody-based elisas. | bacterial cultures from 1801 human diarrhoeal faecal specimens were examined for verocytotoxins i and ii by monoclonal antibody-based sandwich elisas. of the 68 elisa-positive cultures selected from initial screening, 32 were positive by elisa on repeat or from freshly grown cultures. elisa-positive pure cultures were obtained from 13 of these, of which seven were confirmed as verocytotoxin positive by cytotoxicity assay. these seven strains were typed as o26 (5) and o146 (2). the six false posi ... | 1996 | 8606355 |
| identification of tonb homologs in the family enterobacteriaceae and evidence for conservation of tonb-dependent energy transduction complexes. | the transport of fe(iii)-siderophore complexes and vitamin b12 across the outer membrane of escherichia coli requires the tonb-dependent energy transduction system. a set of murine monoclonal antibodies (mabs) was generated against an e. coli trpc-tonb fusion protein to facilitate structure and function studies. in the present study, the epitopes recognized by these mabs were mapped, and their distribution in gram-negative organisms was examined. cross-species reactivity patterns obtained agains ... | 1996 | 8631714 |
| affinity purification and binding characteristics of citrobacter freundii ampr, the transcriptional regulator of the ampc beta-lactamase gene. | the transcriptional regulator of the citrobacter freundii ampc beta-lactamase gene, ampr, was purified as a single sds/page-gel band by using various techniques, including dna-sepharose 4b affinity chromatography. the purified ampr consisted of a 32.5 kda monomer that interacted with three operator sequences: two binding sequences, at positions -75 to -70 and -67 to -51 with respect to the transcriptional start site, were located in the lysr motif (-72 to -60), and the third sequence was at posi ... | 1996 | 8639272 |
| growth suppression in early-stationary-phase nutrient broth cultures of salmonella typhimurium and escherichia coli is genus specific and not regulated by sigma s. | we have studied the growth suppression seen in early-stationary-phase lb broth cultures of salmonella typhimurium. multiplication of small numbers of an antibiotic-resistant s. typhimurium mutant was prevented when the mutant was added to 24-h cultures of the antibiotic-sensitive parent strain, whereas an antibiotic-resistant mutant of an escherichia coli strain added to the same culture grew well. a 24-h e. coli culture produced a similar specific bacteriostatic inhibition against e. coli. in o ... | 1996 | 8655482 |
| assessment of biodegradability of organic acids by a defined microbial mixture. | 1996 | 8661457 | |
| the catalytic mechanism of tyrosine phenol-lyase from erwinia herbicola: the effect of substrate structure on ph-dependence of kinetic parameters in the reactions with ring-substituted tyrosines. | apparently homogeneous tyrosine phenol-lyase (tpl) from erwinia herbicola has been prepared by a new method. the ph-dependencies of the main kinetic parameters for the reactions of erwinia tpl with tyrosine, 2-fluorotyrosine, 3-fluorotyrosine, 2-chlorotyrosine, and 3,4-dihydroxyphenylalanine (dopa) have been studied. the pattern of ph-dependence of v(max) depends on the nature of the substituent in the aromatic ring. for the substrates bearing small substituents (h, 2-f, 3-f) v(max) values were ... | 1996 | 8663898 |
| inhibition of the hemolytic activity of the first component of complement c1 by an escherichia coli c1q binding protein. | molecular mimicry is a well established mechanism via which bacteria protect themselves from complement-mediated killing. we have previously demonstrated that a number of human cells express receptors for c1q (c1qr) and that the soluble form of this receptor inhibits activation of the classical pathway of complement. we now investigated whether escherichia coli possesses a c1qr-like protein that protects these bacteria from complement-mediated injury. by facs analysis it was shown that approxima ... | 1996 | 8666822 |
| belgian multicentre study on the in vitro activity of cefepime against gram-negative bacilli. | the in vitro activity of cefepime has been compared with that of cefotaxime, ceftazidime, aztreonam, and piperacillin against 1826 enterobacteriaceae including 537 inducible enterobacteriaceae (enterobacter spp., serratia spp., citrobacter spp,. morganella morganii) and 572 non-fermenters, including 401 pseudomonas aeruginosa and 111 acinebacter spp. isolated from hospitalized patients in 28 belgian hospitals. overall, cefepime was found substantially more active than the third-generation cephal ... | 1996 | 8669160 |
| the use of a rapid salmonella latex serogrouping test (spectate) to assist in the confirmation of elisa-based rapid salmonella screening tests. | spectate, a 10 min, simple, latex-based agglutination test for serogrouping of salmonellae, has been investigated as a tool to assist in the confirmation of elisa presumptive positive broth samples when screening for salmonellae in foods. when obtaining a combined elisa and spectate positive result, there was a 90% (27/30) confidence limit of a genuine positive result, some one or two working days quicker than the traditional methods. of the 10% (3/30) that were not confirmed as salmonellas, two ... | 1996 | 8672278 |
| susceptibility of bacterial isolates to beta-lactam antibiotics from u.s. clinical trials over a 5-year period. | results are reported for agar dilution susceptibility testing of 3,075 isolates of aerobic bacteria collected from >200 u.s. institutions, located in 30 different states. these isolates were collected from 1987 through 1991 from patients who participated in cefepime clinical trials. cefepime susceptibility was compared with ceftazidime, cefotaxime, ceftriaxone, cefoperazone, and imipenem. to avoid duplication of strains, only initial isolates were included. cefepime minimum inhibitory concentrat ... | 1996 | 8678092 |
| comparative activity of eight antimicrobial agents against clinical bacterial isolates from the united states, measured by two methods. | in a surveillance study conducted during 1992-1993 at 83 medical institutions of different types and sizes (e.g., laboratories, community hospitals, teaching hospitals) and from different geographical areas of the united states, clinical bacterial isolates were tested for their susceptibility to eight comparative antimicrobial agents (cefepime, ceftazidime, cefotaxime, ceftriaxone, ciprofloxacin, gentamicin, imipenem, and piperacillin). a total of 12,574 isolates were tested by either the etest ... | 1996 | 8678094 |
| bacteriologic and clinical applications of a new extended-spectrum parenteral cephalosporin. | although third-generation cephalosporins have been considered the backbone of antibiotic therapy for the treatment of many kinds of serious infections, including those in hospitalized patients, lack of activity against some important pathogens still exists among currently available drugs. in addition, increasing accounts of antibiotic resistance, particularly in the hospital environment, are of deep concern and have thus led to the need for the development of newer antimicrobial agents. cefepime ... | 1996 | 8678097 |
| [variation in transfer resistance to cefotaxime, ceftazidime and aztreonam in strains of acinetobacter sp., enterobacter sp. and citrobacter sp. isolated from the same university hospitals]. | in the last decade were in sr documented new problems in resistance to the newer antibiotics that with regard to their structure and antibacterial properties resisted to the known mechanism of bacterial resistance. the emergence of multiple drug resistance to the new betalactams is connected both with frequent application of these drugs in the hospitals and transfer of r plasmids. | 1996 | 8681362 |
| thermal necrosis after tibial reaming for intramedullary nail fixation. a report of three cases. | we present three young men who sustained closed diaphyseal fracture of the tibia and later developed severe osteocutaneous necrosis induced by heat during intramedullary reaming. they all had a narrow medullary cavity and in all a tourniquet had been used. each developed a pretibial cutaneous blister soon after operation. in the following month severe osteomyelitis ensued, requiring segmental resection and osteocutaneous reconstruction. | 1996 | 8682825 |
| the occurrence of glycine in bacterial lipopolysaccharides. | the aminoacyl analysis of endotoxic lipopolysaccharides (lps) isolated from several bacteria revealed essential amounts of glycine, among the inherent lps components. significant amounts of the glycine was detected in lipopolysaccharides isolated from over 30 strains of escherichia, salmonella, hafnia, citrobacter and shigella species. glycine as a single amino acid was found only in a core part of lps. molar ratio of glycine in core oligosaccharide fraction ranged from 0.2 to 0.6 per 3 heptoses ... | 1996 | 8739188 |
| distribution of diaminopropane and acetylspermidine in enterobacteriaceae. | polyamines of 97 strains (60 species) belonging to 18 genera of the family enterobacteriaceae were determined by high performance liquid chromatographic analysis. in addition to putrescine and cadavarine, diaminopropane was widely distributed in enterobacteriaceae and almost ubiquitously within enterobacter, pantoea, erwinia, leminorella, proteus, leclercia, morganella, klebsiella, hafnia, rahnella, serratia, and tatumella species and sporadically within citrobacter, escherichia, moellerella, pr ... | 1996 | 8742354 |
| outbreak of tem-24-producing enterobacter aerogenes in an intensive care unit and dissemination of the extended-spectrum beta-lactamase to other members of the family enterobacteriaceae. | we report an outbreak of enterobacter aerogenes in an intensive care unit (icu) and two medicine departments that produced the extended-spectrum beta-lactamase tem-24, which was difficult to detect by disk agar diffusion. the strains were compared by dna restriction fragment length polymorphism after pulsed-field gel electrophoresis following cleavage with xbai. this typing method indicated that a single strain, first isolated in the icu, spread throughout the other medical departments as a resu ... | 1996 | 8748277 |
| comparative evaluation of orally active antibiotics against community-acquired pathogens: results of eight european countries. | in this multicenter study conducted in eight european countries, 13,173 pathogens--all isolated from community-acquired infections in 1992 and 1993--were evaluated for their susceptibility to the following orally active antibiotics: penicillin g, ampicillin, amoxycillin plus clavulanic acid, cefaclor, cefuroxime, cefetamet, doxycycline and erythromycin. ten centers in italy, five in germany, in the netherlands and switzerland, four in greece and spain, three in hungary and one in finland contrib ... | 1996 | 8751263 |
| [comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (1994). i. susceptibility distribution]. | the frequencies of isolation and susceptibilities to antimicrobial agents were investigated on 567 bacterial strains isolated from patients with urinary tract infections in 10 hospitals during the period of june 1994 to may 1995. of the above total bacterial isolates, gram-positive bacteria accounted for 26.8% and a majority of them were enterococcus faecalis. gram-negative bacteria accounted for 73.2% and most of them were escherichia coli. 1. enterococcus faecalis. ampicillin (abpc) and imipen ... | 1996 | 8752862 |
| [in vitro study of antibacterial activity of cefepime (axepim) against gram negative bacteria: comparison with cephalosporins and other beta-lactams]. | the susceptibility to cefepime and to other beta-lactams of 1017 inducible cephalosporinase-producing enterobacteria, 897 pseudomonas aeruginosa strains, and 295 acinetobacter baumanii strains was studied over a two-year period (july 1, 1993 to june 30, 1995). the isolates were from patients in visceral surgery, intensive care, and clinical hematology wards. cefepime was compared to other third-generation cephalosporins and to imipenem, aztreonam, and the piperacillin-tazobactam combination. cef ... | 1996 | 8761598 |
| citrobacter emphysematous pyelonephritis in a tuberculous kidney caused by citrobacter. a case report in a diabetic patient. | emphysematous pyelonephritis caused by gas-producing bacteria like escherichia coli or klebsiella pneumonia is generally observed in female diabetic patients. we report a case in which citrobacter was the microbiologically documented pathogen. high-dose antibiotic regimen was administered, but nephrectomy was necessary to overcome the life-threatening situation. | 1996 | 8766145 |
| [comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (1994). iii. secular changes in susceptibility]. | susceptibilities of enterococcus faecalis, staphylococcus aureus, citrobacter spp., enterobacter spp., escherichia coli, klebsiella spp., proteus mirabilis, pseudomonas aeruginosa and serratia spp. isolated from patients with urinary tract infections (utis) in 10 hospitals during june 1994 to may 1995 to various antimicrobial agents were compared with those in the same period of previous years according to a classification, uncomplicated utis, complicated utis without indwelling catheter, and co ... | 1996 | 8776631 |
| favorable outcome possible in citrobacter brain abscess. | 1996 | 8783364 | |
| evaluation of chromagar orientation for differentiation and presumptive identification of gram-negative bacilli and enterococcus species. | a new chromogenic plate medium, chromagar orientation, was evaluated for use in the differentiation and presumptive identification of gram-negative bacilli and enterococcus species by a multipoint inoculation (replicator) technique. in this study, 1,404 gram-negative bacilli and 74 enterococcal isolates were tested on chromagar orientation. six control american type culture collection strains were also included with the testing to ensure quality control of the media. of the escherichia coli isol ... | 1996 | 8784591 |
| [in vitro antibacterial activity of a new parenteral penem, sulopenem]. | eighty percent minimum inhibitory concentrations (mic80) of sulopenem against clinically isolated 12 to 80 strains of each of different bacteria were as follows: methicillin-susceptible staphylococcus aureus (mssa): 0.20 micrograms/ml, methicillin-resistant s. aureus (mrsa): 50 micrograms/ml, coagulase-negative staphylococci: 3.13 micrograms/ml, streptococcus pyogenes: < or = 0.013 microgram/ml, streptococcus pneumoniae: < or = 0.013 microgram/ml, beta-streptococci: 0.05 microgram/ml, enterococc ... | 1996 | 8786624 |
| [antibacterial activity of sulopenem, a new parenteral penem antibiotic]. | sulopenem, a new penem antibiotic, was compared with other antibiotics with regard to in vitro antibacterial and bactericidal activities, stabilization against beta-lactamases, and effect on the release of lipopolysaccharide from gram-negative bacteria. the results are summarized as follows. 1. sulopenem showed more potent activities than other antibiotics against both gram-positive and gram-negative bacteria except pseudomonas aeruginosa. 2. sulopenem showed potent bactericidal activities (mic/ ... | 1996 | 8786625 |
| [in vitro and in vivo antibacterial activities of sulopenem, a new penem antibiotic]. | the in vitro and in vivo antibacterial activities of sulopenem, a new penem, were evaluated in comparison with imipenem (ipm), meropenem (mepm), ceftazidime (caz) and flomoxef (fmox). sulopenem had broad and potent antibacterial spectra against gram-positive and gram-negative bacteria, including enterococcus faecalis, proteus vulgaris, morganella morganii, enterobacter spp. and citrobacter freundii. sulopenem showed concentration-dependent bactericidal activities against staphylococcus aureus, e ... | 1996 | 8786626 |
| characterization of the plasmidic beta-lactamase cmy-2, which is responsible for cephamycin resistance. | the phenotype of klebsiella pneumoniae hel-1 indicates a plasmidic cephamycinase gene (blacmy-2). its sequence shows one open reading frame coding for a protein of 381 amino acids. cmy-2 is classified as class c beta-lactamase that is closely related to the plasmidic enzymes bil-1 and lat-1 and the chromosomal ampc of citrobacter freundii. the blacmy-2 gene possibly was translocated onto a plasmid of c. freundii which spread to k. pneumoniae. | 1996 | 8787910 |
| confirmational identification of escherichia coli, a comparison of genotypic and phenotypic assays for glutamate decarboxylase and beta-d-glucuronidase. | genotypic and phenotypic assays for glutamate decarboxylase (gad) and beta-d-glucuronidase (gud) were compared for their abilities to detect various strains of escherichia coli and to discriminate among other bacterial species. test strains included nonpathogenic e. coli, three major groups of diarrheagenic e. coli, three other non-coli escherichia species, and various other gram-negative and -positive bacteria found in water. the genotypic assays were performed with hybridization probes generat ... | 1996 | 8795225 |
| mutational analysis of the cita citrate transporter from salmonella typhimurium: altered substrate specificity. | the cita citrate transporter in salmonella typhimurium is encoded by the cita gene and consists of 434 amino acid residues that probably include 12 membrane-spanning segments [shimamoto. t., et al. (1991) j. biochem. 110, 22-28]. cita mutants with altered substrate specificities were isolated by in vitro mutagenesis using nitrous acid. the mutants could grow on isocitrate as a sole carbon source which normally cannot be transported well by the cita transporter of s. typhimurium. the mutation sit ... | 1996 | 8806660 |
| transferable cefoxitin resistance in enterobacteria from greek hospitals and characterization of a plasmid-mediated group 1 beta-lactamase (lat-2). | cefoxitin resistance in klebsiella pneumoniae from escherichia coli strains isolated in greek hospitals was found to be due to the acquisition of similar plasmids coding for group 1 beta-lactamases. the plasmids were not self-transferable but were mobilized by conjugative plasmids. these elements have also been spread to enterobacter aerogenes. the most common enzyme was a citrobacter freundii-derived cephalosporinase (lat-2) which differed from lat-1 by three amino acids. | 1996 | 8807075 |
| design, synthesis, and evaluation of 2 beta-alkenyl penam sulfone acids as inhibitors of beta-lactamases. | a general method for synthesis of 2 beta-alkenyl penam sulfones has been developed. the new compounds inhibited most of the common types of beta-lactamase. the level of activity depended very strongly on the nature of the substituent in the 2 beta-alkenyl group. the inhibited species formed with the beta-lactamase from citrobacter freundii 1205 was sufficiently stable for x-ray crystallographic studies. these, together with uv absorption spectroscopy and studies of chemical degradation, suggeste ... | 1996 | 8809160 |
| structural and serological studies of lipopolysaccharides of citrobacter o35 and o38 antigenically related to salmonella. | structural analysis using 13c nmr spectroscopy and methylation showed that lipopolysaccharides (lpss) of citrobacter freundii o35 and salmonella arizonae o59 have structurally identical o-specific polysaccharide chains, and those of c. freundii o38 and salmonella kentucky differ only in the presence of o-acetyl groups in the former. serological relationships between the structurally similar lpss were demonstrated using inhibition of elisa, rocket immunoelectrophoresis, double gel diffusion, and ... | 1996 | 8821392 |
| characterization and expression of the plasmid-borne bedd gene from pseudomonas putida ml2, which codes for a nad+-dependent cis-benzene dihydrodiol dehydrogenase. | the catabolic plasmid phmt112 in pseudomonas putida ml2 contains the bed gene cluster encoding benzene dioxygenase (bedc1c2ba) and a nad+-dependent dehydrogenase (bedd) required to convert benzene into catechol. analysis of the nucleotide sequence upstream of the benzene dioxygenase gene cluster (bedc1c2ba) revealed a 1,098-bp open reading frame (bedd) flanked by two 42-bp direct repeats, each containing a 14-bp sequence identical to the inverted repeat of is26. in vitro translation analysis sho ... | 1996 | 8824602 |
| cloning, sequencing, and overexpression of the genes encoding coenzyme b12-dependent glycerol dehydratase of citrobacter freundii. | the genes encoding coenzyme b12-dependent glycerol dehydratase of citrobacter freundii were cloned and overexpressed in escherichia coli. the b12-free enzyme was purified to homogeneity. it consists of three types of subunits whose n-terminal sequences are in accordance with those deduced from the open reading frames dhab, dhac, and dhae, coding for subunits of 60,433 (alpha), 21,487 (beta), and 16,121 (gamma) da, respectively. the enzyme complex has the composition alpha2beta2gamma2. amino acid ... | 1996 | 8824629 |
| use of two 16s dna targeted oligonucleotides as pcr primers for the specific detection of salmonella in foods. | a 16s dna targeted polymerase chain reaction (pcr) method specific for the detection of salmonella isolates with various serotypes was developed. the primers used for such a pcr method were 16sf1 and 16siii. 16sf1 is the reverse and complementary strand of 16si which has been shown to be able to hybridize with salmonella and citrobacter spp. 16iii on the other hand, is able to hybridize with klebsiella and serratia spp. in addition to salmonella. although 16sf1 and 16siii were not specific to sa ... | 1996 | 8698667 |
| chromosomal beta-lactamase genes of klebsiella oxytoca are divided into two main groups, blaoxy-1 and blaoxy-2. | the chromosomally encoded beta-lactamase gene (blaoxy-2) of the wild-type klebsiella oxytoca sl911 was cloned and sequenced. its nucleotide sequence similarity with the previously sequenced k. oxytoca beta-lactamase gene (blaoxy-1) (y. arakawa, m. ohta, n. kido, m. mori, h. ito, t. komatsu, y. fujii, and n. kato, antimicrob. agents chemother. 33:63-70, 1989) is 87.3%, and its amino acid similarity is 89.7%. this group of k. oxytoca beta-lactamases is related to chromosomal beta-lactamases of cit ... | 1996 | 8834897 |
| comparative characterization of the cephamycinase blacmy-1 gene and its relationship with other beta-lactamase genes. | a plasmidic beta-lactamase which hydrolyzed cephamycins was first detected and reported in 1989. at that time its description was restricted to phenotypic characteristics. we analyzed nucleotide sequence of its gene and explored it genetic relationship with other bla genes. the deduced amino acid sequence of the blacmy-1 product was compared with those of other known plasmidic cephamycinases and of chromosomal ampc beta-lactamases. the results indicate that the relationship of cmy-1 is closest t ... | 1996 | 8843306 |
| a new time-kill method of assessing the relative efficacy of antimicrobial agents alone and in combination developed using a representative beta-lactam, aminoglycoside and fluoroquinolone. | a time-kill curve employing nine sampling times over 6 h was used to provide data which were then used to develop a theoretical (best-fit) curve. from the theoretical curve parameters describing the rate of kill (alpha), time from addition of antibiotic to initiation of killing (d) and a function of the degree of killing observed (ym/yo) were defined. the area-under-the-curve (auc) was calculated from the theoretical curve. the variability of each parameter was assessed using a theoretical curve ... | 1996 | 8877533 |
| bacteremia due to citrobacter species: significance of primary intraabdominal infection. | from 1982 to 1994, 45 patients (1.22 episodes per 10,000 discharged patients) were treated for citrobacter bacteremia at national taiwan university hospital (taipei). all patients had at least one underlying disease. citrobacter bacteremia most commonly occurred in patients with malignancies (48.9%) or hepatobiliary stones (22.2%). intraabdominal tumors comprised the majority (59.1%) of malignancies. bacteremia commonly originated from sites such as the abdominal cavity (51.1%), urinary tract (2 ... | 1996 | 8879778 |
| risk factors for nosocomial infections caused by gram-negative bacilli. the hellenic antibiotic resistance study group. | two hundred and ninety-nine gram-negative hospital-acquired infections from 257 patients, consecutively identified during one month (november 1992) in five hospitals in the greater athens area, were divided into four groups on the basis of the bacterium isolated: group 1 (escherichia coli group) included infections owing to e. coli, group 2 (proteus group) consisted of infections owing to proteus spp. and providencia spp., group 3 (kiebsiella/enterobacter group) involved infections owing to kieb ... | 1996 | 8880546 |
| royal society of tropical medicine and hygiene meeting at manson house, london, 14 december 1995. enteropathogenic escherichia coli--mucosal infection models. | the formation of attaching and effacing (a/e) lesions is central to the pathogenesis of enteropathogenic escherichia coli (epec)-mediated disease in humans and citrobacter rodentium-mediated transmissible colonic hyperplasia in mice. closely related outer membrane proteins, known as intimins, are required for formation of the a/e lesion by both epec and c. rodentium. in this study we found similar ultrastructural damage in small intestinal biopsies from an epec-infected child and large bowel spe ... | 1996 | 8882174 |
| a simple method to detect bacteriolytic enzymes produced by enterobacteriaceae. | the production of bacteriolytic enzymes by enterobacteriaceae in various growth conditions was investigated. peptone-based media containing killed gram-negative cells facilitated detection of bacteriolytic enzyme production in the highest number of species. these belonged to the genera serratia, proteus, morganella and providencia. in contrast, escherichia coli, shigella, salmonella, klebsiella, enterobacter and citrobacter species did not produce bacteriolytic activities in any of the condition ... | 1996 | 8893522 |
| characterization of the eaea gene from rabbit enteropathogenic escherichia coli strain rdec-1 and comparison to other eaea genes from bacteria that cause attaching-effacing lesions. | a number of enteric pathogens, including enteropathogenic (epec) and enterohemorrhagic (ehec) escherichia coli, hafnia alvei, a strain of citrobacter freundii, and rabbit epec strain rdec-1 cause attaching-effacing (ae) lesions in the gut mucosa. these bacteria have a pathogenicity cassette (locus of enterocyte effacement or lee) containing the eaea gene. this gene encodes intimin, an outer membrane protein required for production of ae lesions. rdec-1, a non-invasive enteropathogen in young rab ... | 1996 | 8900070 |
| [evaluation of a new disk containing indoxyl-beta-d-glucuronide for rapid identification of escherichia coli]. | to establish a simple identification procedure for escherichia coli, we developed a disk containing indoxyl-beta-d-glucuronide, the chromogenic substrate of beta-d-glucuronidase. of 188 isolates of enterobacteriaceae, 101 (97%) of 104 strains of e. coli and two (67%) of three strains of shigella species were positive for beta-glucuronidase. we also tested 495 strains (466 strains of e. coli and 29 of citrobacter freundii) that might be considered lactose-fermenting e. coli under routine conditio ... | 1996 | 8911077 |
| occurrence of a novel l-2,4-diaminobutyrate decarboxylase activity in some species of enterobacteriaceae, and purification and characterization of the enzymes of enterobacter aerogenes and serratia marcescens. | l-2,4-diaminobutyrate decarboxylase (daba dc) is a novel enzyme yielding 1,3-diaminopropane (dap) from daba, which has previously been purified from strains of the genera vibrio and acinetobacter. in this study, we also detected daba dc activity in the species of enterobacteriaceae: e. aerogenes, e. cloacae, e. agglomerans, serratia marcescens, s. liquefaciens, klebsiella pneumoniace, k. oxytoca and citrobacter freundii, all of which produced dap in sufficient amounts. subsequently, the daba dcs ... | 1996 | 8913500 |
| 3-hydroxypropionaldehyde, an inhibitory metabolite of glycerol fermentation to 1,3-propanediol by enterobacterial species. | glycerol fermentation by enterobacter agglomerans revealed that both growth and 1,3-propanediol production ceased after consumption of about 430 mm glycerol, irrespective of the initial glycerol content. this phenomenon was assigned to the production of 3-hydroxypropionaldehyde, which was identified by proton nuclear magnetic resonance and which showed a bacteriostatic effect. the accumulation during glycerol fermentation was also observed with two other enterobacterial species, i.e., klebsiella ... | 1996 | 8919810 |
| the influence of incubation conditions on the adherence of oral enterobacteriaceae to hela cells. | the adherence of 12 oral isolates and 4 type strains of enterobacteriaceae (equally representing enterobacter cloacae, klebsiella pneumoniae, escherichia coli and citrobacter freundii) to hela cell monolayers following five different incubation conditions (sucrose, d-mannose, serum, mem and candida albicans gdh 1957) was investigated. incubation with sucrose and d-mannose resulted in the greatest and least adherence, respectively. the presence of preadherent c. albicans gdh 1957 on the hela cell ... | 1996 | 8920813 |
| [enterobacteria isolated from commercial fishes from the volga-caspian basin]. | enteric bacteria, such as proteus, citrobacter [correction of cytobacter], and providencia, were found to be prevalent particularly in the gills and intestines of marketable fishes of the volga and caspian seas. half of all the enteric bacteria was detected in the fish kidneys, spleen, and liver. this shows it necessary to standardize different types of fish raw materials. | 1996 | 8925957 |
| [use of aztreonam for the purpose of preventing postoperative purulent complications]. | the efficacy of the prophylactic use of azthreonam, a monocyclic beta lactam antibiotic, was studied in 20 patients operated for liver diseases. the drug was administered intramuscularly in a dose of 1 g 30 minutes prior to the operation. during the postoperative period the drug was administered in a dose of 1 g 4-5 times a day for 4 to 5 days. the clinical effect in all the patients was good: no elevation of the body temperature above 37.5 degrees c and no suppuration of the operation wound. ad ... | 1996 | 8929118 |
| characterization of espc, a 110-kilodalton protein secreted by enteropathogenic escherichia coli which is homologous to members of the immunoglobulin a protease-like family of secreted proteins. | enteropathogenic escherichia coli (epec) secretes at least five proteins. two of these proteins, espa and espb (previously called eaeb), activate signal transduction pathways in host epithelial cells. while the role of the other three proteins (39, 40, and 110 kda) remains undetermined, secretion of all five proteins is under the control of pera, a known positive regulator of several epec virulence factors. on the basis of amino-terminal protein sequence data, we cloned and sequenced the gene wh ... | 1996 | 8932311 |