Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| microbiota-mediated inflammation and antimicrobial defense in the intestine. | the diverse microbial populations constituting the intestinal microbiota promote immune development and differentiation, but because of their complex metabolic requirements and the consequent difficulty culturing them, they remained, until recently, largely uncharacterized and mysterious. in the last decade, deep nucleic acid sequencing platforms, new computational and bioinformatics tools, and full-genome characterization of several hundred commensal bacterial species facilitated studies of the ... | 2015 | 25581310 |
| respiratory paralysis in a child: the severe axonal variant of childhood guillain-barré syndrome. | guillain-barre syndrome (gbs) is a common cause of acute flaccid paralysis in children. axonal variants of this disease are rare, and frequently life-threatening or debilitating. the course and outcome of a 17-month-old child with acute flaccid paralysis including severe respiratory involvement are presented. gbs was suspected. nerve conduction studies demonstrated acute motor-sensory axonal neuropathy including both phrenic nerves. the difficulties with the diagnosis and management of this seve ... | 2015 | 25878751 |
| the role of the gut microbiota in the pathogenesis of antiphospholipid syndrome. | infectious triggers are associated with the induction of transient antiphospholipid antibodies. one therefore wonders if microbes that permanently colonize us play a role in the pathogenesis of antiphospholipid syndrome (aps). the microbiota represents the collection of all microorganisms colonizing humans and is necessary for normal host physiology. the microbiota, however, is a constant stress on the immune system, which is tasked with recognizing and eliminating pathogenic microbes while tole ... | 2015 | 25475595 |
| pediatric guillain-barré syndrome: indicators for a severe course. | this study aims to retrospectively evaluate pediatric guillain-barré syndrome cases in a tertiary center in istanbul, turkey. | 2015 | 25745306 |
| guillain - barre syndrome in a patient with acute myocardial infarction with ventricular septal defect repair treated with plasma exchange. | guillain - barre syndrome (gbs) is an acute, frequently severe progressive illness of peripheral nervous system that is autoimmune in nature. gbs after myocardial infarction (mi) with ventricular septal defect (vsd) is uncommon with high mortality rate if not treated promptly.[1] we report a successful outcome of gbs post mi with vsd in a 60-year-old male patient who was on a ventilator treated successfully with therapeutic plasma exchange. | 2015 | 25722582 |
| enteropathogens detected in a daycare center, southeastern brazil: bacteria, virus, and parasite research. | the objective of this study was to determine the prevalence and etiological profile of enteropathogens in children from a daycare center. | 2015 | 25651323 |
| cloning, expression, refolding, purification and preliminary crystallographic analysis of the sensory domain of the campylobacter chemoreceptor for aspartate a (ccaa). | in campylobacter jejuni, chemotaxis and motility have been identified as important virulence factors that are required for host colonization and invasion. chemotactic recognition of extracellular signals is mediated by the periplasmic sensory domains of its transducer-like proteins (tlps). in this study, the sensory domain of the c. jejuni chemoreceptor for aspartate a (ccaa) has been expressed in escherichia coli and purified from inclusion bodies. the urea-denatured protein was refolded and th ... | 2015 | 25615981 |
| participatory evaluation of chicken health and production constraints in ethiopia. | chicken production has a major role in the economy of developing countries and backyard production is particularly important to women. several programmes, in ethiopia and elsewhere, have attempted to improve chicken production as a means to reduce poverty. a key constraint to chicken production identified by farmers is disease. this study used participatory rural appraisal methods to work with chicken-keepers in order to prioritise chicken diseases, place these within the context of other produc ... | 2015 | 25466215 |
| chemical composition and antimicrobial activity of essential oils of juniperus excelsa bieb. (cupressaceae) grown in r. macedonia. | there are no information of the yield, chemical composition and antimicrobial activity of essential oils of berries (eob) or leaves (eol) of juniperus excelsa bieb. (cupressaceae) growing wild in r. macedonia. | 2015 | 25598638 |
| commentary. | 2015 | 25552856 | |
| mechanisms of bacterial morphogenesis: evolutionary cell biology approaches provide new insights. | how darwin's "endless forms most beautiful" have evolved remains one of the most exciting questions in biology. the significant variety of bacterial shapes is most likely due to the specific advantages they confer with respect to the diverse environments they occupy. while our understanding of the mechanisms generating relatively simple shapes has improved tremendously in the last few years, the molecular mechanisms underlying the generation of complex shapes and the evolution of shape diversity ... | 2015 | 25664446 |
| genome-wide gene order distances support clustering the gram-positive bacteria. | initially using 143 genomes, we developed a method for calculating the pair-wise distance between prokaryotic genomes using a monte carlo method to estimate the conservation of gene order. the method was based on repeatedly selecting five or six non-adjacent random orthologs from each of two genomes and determining if the chosen orthologs were in the same order. the raw distances were then corrected for gene order convergence using an adaptation of the jukes-cantor model, as well as using the co ... | 2015 | 25653643 |
| genome-wide gene order distances support clustering the gram-positive bacteria. | initially using 143 genomes, we developed a method for calculating the pair-wise distance between prokaryotic genomes using a monte carlo method to estimate the conservation of gene order. the method was based on repeatedly selecting five or six non-adjacent random orthologs from each of two genomes and determining if the chosen orthologs were in the same order. the raw distances were then corrected for gene order convergence using an adaptation of the jukes-cantor model, as well as using the co ... | 2015 | 25653643 |
| pcr for enteric pathogens in high-prevalence settings. what does a positive signal tell us? | molecular methods, in particular pcr, are increasingly used for the diagnosis of enteric pathogens in stool samples. in high-endemicity settings, however, asymptomatic carriage or residual dna from previous infections will hamper the interpretation of positive test results. we assessed the quantitative dimension of this problem in schoolchildren in the rural highlands of madagascar. | 2015 | 25761823 |
| [detection of biofilm formation by selected pathogens relevant to the food industry]. | detection of biofilm formation by microbial pathogens relevant to the food industry and comparison of biofilm formation under different conditions of culture. | 2015 | 26448305 |
| antibiotic-resistant bacteria: prevalence in food and inactivation by food-compatible compounds and plant extracts. | foodborne antibiotic-resistant pathogenic bacteria such as campylobacter jejuni, bacillus cereus, clostridium perfringens, escherichia coli, salmonella enterica, staphylococcus aureus, vibrio cholerae, and vibrio parahemolyticus can adversely affect animal and human health, but a better understanding of the factors involved in their pathogenesis is needed. to help meet this need, this overview surveys and interprets much of our current knowledge of antibiotic (multidrug)-resistant bacteria in th ... | 2015 | 25856120 |
| microbiological diversity and prevalence of spoilage and pathogenic bacteria in commercial fermented alcoholic beverages (beer, fruit wine, refined rice wine, and yakju). | the present study examined 469 commercially available fermented alcoholic beverages (fabs), including beer (draft, microbrewed, and pasteurized), fruit wine (grape and others), refined rice wine, and yakju (raw and pasteurized). samples were screened for escherichia coli and eight foodborne pathogens (bacillus cereus, campylobacter jejuni, clostridium perfringens, escherichia coli o157:h7, listeria monocytogenes, salmonella spp., staphylococcus aureus, and yersinia enterocolitica), and the aerob ... | 2015 | 25836410 |
| antimicrobial impacts of essential oils on food borne-pathogens. | the antimicrobial activity of twelve essential oil (pine oil, eucalyptus, thyme, sage tea, lavender, orange, laurel, lemon, myrtle, lemon, rosemary and juniper) was tested by a disc diffusion method against food borne pathogens (escherichia coli, salmonella paratyphi a, klebsiella pneumoniae, yersinia enterocolitica, pseudomonas aeruginosa, aeromonas hydrophila, campylobacter jejuni, enterococcus faecalis, staphylococcus aureus). the major components in essential oils were monoterpenes hydrocarb ... | 2015 | 26072990 |
| association between acute motor axonal neuropathy and septic shock due to acinetobacter baumannii. | in this report, we describe a case of acute motoral axonal neuropathy (aman) following septic shock due to acinetobacter baumannii. the aetiology of aman is still not fully clarified. an association with a potential infection by campylobacter jejuni, resulting in stimulation of autoimmune response against gangliosides mediated by a phenomenon of molecular mimicry, is believed to play a major role. since the lipopolysaccharide of a. baumannii has a structure that is similar to that of c. jejuni, ... | 2015 | 26700086 |
| conventional and molecular methods in the diagnosis of community-acquired diarrhoea in children under 5 years of age from the north-eastern region of poland. | the purpose of this study was to determine the main causative agents of community-acquired acute diarrhoea in children using conventional methods and pcr. | 2015 | 26159845 |
| [identification of zoonotic bacterial pathogens by the maldi tof ms method]. | to verify whether the maldi tof ms method can be used for rapid identification of selected zoonotic bacterial pathogens isolated from various types of materials in the real conditions of routine laboratory work. | 2015 | 26312374 |
| management of the marginal zone lymphomas. | marginal zone lymphomas (mzl) represent around 8 % of all non-hodgkin lymphomas. during the last decades a number of studies have addressed the mechanisms underlying the disease development. extranodal mzl lymphoma usually arises in mucosal sites where lymphocytes are not normally present from a background of either autoimmune processes, such as hashimoto thyroiditis or sjögren syndrome or chronic infectious conditions. in the context of a persistent antigenic stimulation, successive genetic abn ... | 2015 | 25655612 |
| prevalence and antimicrobial resistance of campylobacterjejuni and campylobacter coli from adult hospitalized patients with diarrhea in thailand. | infection with campylobacter jejuni and c. coli are recognized as the major cause of bacterial gastroenteritis in humans. | 2015 | 26749592 |
| eucast recommendations for antimicrobial susceptibility testing applied to the three main campylobacter species isolated in humans. | antimicrobial susceptibility testing of campylobacter isolates is of great importance for treatment options especially in systemic diseases. the european committee for antimicrobial susceptibility testing (eucast) recently proposed epidemiological cut-offs (ecoffs) for a limited number of antimicrobial compounds and for campylobacter jejuni and campylobacter coli only. in the present study, the eucast method was used after minor modifications to define antimicrobial susceptibility patterns for, ... | 2015 | 26519770 |
| efficacy of a typing scheme for campylobacter based on the combination of true and questionable crispr. | this study evaluates an improved scheme for campylobacter genotyping based on the combination of true and questionable crispr (clustered regularly interspaced short palindromic repeats) elements. a total of 180 campylobacter strains (campylobacter jejuni n=93 and campylobacter coli n=87), isolated from neck skin and caecal content of broilers, poultry meat and sewage water were analysed. another 97 c. jejuni dna samples from cases of human campylobacteriosis were assessed. sixty-three genotypes ... | 2015 | 26518609 |
| campylobacter in poultry: ecology and potential interventions. | avian hosts constitute a natural reservoir for thermophilic campylobacter species, primarily campylobacter jejuni and campylobacter coli, and poultry flocks are frequently colonized in the intestinal tract with high numbers of the organisms. prevalence rates in poultry, especially in slaughter-age broiler flocks, could reach as high as 100% on some farms. despite the extensive colonization, campylobacter is essentially a commensal in birds, although limited evidence has implicated the organism a ... | 2015 | 26473668 |
| campylobacter species in animal, food, and environmental sources, and relevant testing programs in canada. | campylobacter species, particularly thermophilic campylobacters, have emerged as a leading cause of human foodborne gastroenteritis worldwide, with campylobacter jejuni, campylobacter coli, and campylobacter lari responsible for the majority of human infections. although most cases of campylobacteriosis are self-limiting, campylobacteriosis represents a significant public health burden. human illness caused by infection with campylobacters has been reported across canada since the early 1970s. m ... | 2015 | 26422448 |
| risk of escherichia coli o157:h7, non-o157 shiga toxin-producing escherichia coli, and campylobacter spp. in food animals and their products in qatar. | escherichia coli o157:h7, non-o157 e. coli, and campylobacter spp. are among the top-ranked pathogens that threaten the safety of food supply systems around the world. the associated risks and predisposing factors were investigated in a dynamic animal population using a repeat-cross-sectional study design. animal and environmental samples were collected from dairy and camel farms, chicken processing plants, and abattoirs and analyzed for the presence of these pathogens using a combination of bac ... | 2015 | 26408129 |
| antimicrobial susceptibilities of campylobacter jejuni and campylobacter coli recovered from organic turkey farms in germany. | the popularity of food produced from animals kept under an organic regimen has increased in recent years. in germany, turkey meat consumption has increased. despite several studies assessing the susceptibility of campylobacters to various antibiotics in poultry, no sufficient data exists regarding the antimicrobial resistance of campylobacters in organic-reared turkeys. this study provides information about antibiotic resistance in campylobacter isolated from turkeys reared on organic farms in g ... | 2015 | 26371330 |
| campylobacter coli infection causing second trimester intrauterine growth restriction (iugr): a case report and review of the literature. | campylobacter is a gram-negative, microaerophilic, curved rod and a normal resident of the gastrointestinal flora and may be the cause of disease in animals. transmission to humans occurs by ingestion of contaminated food or by direct contact with infected animals. in the past few decades, an increasing number of reports have implicated the presence of this organism in human abortions as well. an infectious mechanism due to primary placental inflammatory damage followed by secondary damage to th ... | 2015 | 26340229 |
| antibiotic susceptibility of clinical isolates of campylobacter jejuni and campylobacter coli in ontario, canada during 2011-2013. | a total of 219 clinical isolates of campylobacter spp. including 180 campylobacter jejuni and 39 campylobacter coli were assessed for in vitro antimicrobial susceptibility. resistance among c. coli was higher for ciprofloxacin (41% versus 30.80%), erythromycin (12.80% versus 3.90%) and lower for tetracycline (53.80% versus 64.60%) compared to c. jejuni. | 2015 | 26320936 |
| development of a novel chromogenic medium for improved campylobacter detection from poultry samples. | the presence of expanded-spectrum β-lactamase (esbl)-producing escherichia coli is a common problem in the isolation of campylobacter from poultry samples using conventional cefoperazone-based selective media. a novel chromogenic medium (cm-ht), based on modified charcoal cefoperazone deoxycholate agar (mccda), has been developed as a solution for improved campylobacter detection from poultry samples. although the basic components of cm-ht are the same as mccda, cm-ht uses both granular charcoal ... | 2015 | 26319731 |
| are campylobacters now capable of carbo-loading? | campylobacters are a leading cause of gastrointestinal morbidity worldwide and the majority of human infections are triggered by eating foods contaminated with campylobacter jejuni or campylobacter coli. campylobacters are equally notorious for their ability to mimic human glycoconjugate structures and for their capacity to synthesize both n- and o-linked glycoproteins. these species were once considered to be asaccharolytic, but it was recently shown that several strains possess a pathway for f ... | 2015 | 26259768 |
| high prevalence and genetic diversity of campylobacter jejuni in wild crows and pigeons. | the occurrence, seasonal variation and genetic diversity of campylobacter spp. in pigeons and crows over a 1-year period were evaluated. campylobacter spp. were isolated from 166 (34.6 %) out of 480 wild bird faecal samples. the occurrence of campylobacter spp. in faecal samples was higher among crows (39.2 %) than pigeons (30.0 %), (p < 0.05). campylobacter jejuni was the most common species detected among wild bird faecal samples (98.2 %). meanwhile, campylobacter coli prevalence in wild bird ... | 2015 | 26228635 |
| a new variant of cytolethal distending toxin in a clinical isolate of campylobacter hyointestinalis. | increasing numbers of campylobacter hyointestinalis have been isolated from humans and animals with gastroenteritis, although the virulence mechanism of this species remains largely unknown. here, we show that c. hyointestinalis isolated from a patient with diarrhoea in thailand produced a novel variant of cytolethal distending toxin (cdt). sequencing of a 13 965 bp genomic region of c. hyointestinalis carrying the genes coding for ch-cdt revealed three orfs of 798, 804 and 537 bp, which code fo ... | 2015 | 26220191 |
| adherence reduction of campylobacter jejuni and campylobacter coli strains to hep-2 cells by mannan oligosaccharides and a high-molecular-weight component of cranberry extract. | campylobacter infections are a leading cause of human bacterial gastroenteritis in the united states and are a major cause of diarrheal disease throughout the world. colonization and subsequent infection and invasion of campylobacter require that the bacteria adhere to the surface of host cells. agents that inhibit adherence could be used prophylactically to reduce campylobacter carriage and infection. mannan oligosaccharides (mos) have been used as a feed supplement in livestock animals to impr ... | 2015 | 26219363 |
| microarray on digital versatile disc for identification and genotyping of salmonella and campylobacter in meat products. | highly portable, cost-effective, and rapid-response devices are required for the subtyping of the most frequent food-borne bacteria; thereby the sample rejection strategies and hygienization techniques along the food chain can be tailor-designed. here, a novel biosensor is presented for the generic detection of salmonella and campylobacter and the discrimination between their most prevalent serovars (salmonella enteritidis, salmonella typhimurium) and species (campylobacter jejuni, campylobacter ... | 2015 | 26198111 |
| rapid detection of campylobacter jejuni, campylobacter coli, and campylobacter lari in fresh chicken meat and by-products in bangkok, thailand, using modified multiplex pcr. | a multiplex pcr assay for simultaneous detection and differentiation of campylobacter jejuni, campylobacter coli, and campylobacter lari was developed and validated to assess the occurrence of these bacteria in fresh chicken meat and by-products in bangkok, thailand, by using a new combination of four previously published pcr primers for c. jejuni, c. coli, c. lari, and a universal 16s rdna gene as an internal control. the specificity was determined by using 13 strains of other bacteria. with pu ... | 2015 | 26197289 |
| real-time pcr detection of campylobacter spp.: a comparison to classic culturing and enrichment. | the major disadvantage of the current gold standard for detection of the food pathogen campylobacter, i.e. culturing, is the lengthy procedure. in this study we assessed the use of real-time pcr for detection of campylobacter. to this end, 926 poultry samples, taken from transport containers and broiler caeca in the netherlands in 2007, were subjected to three different real-time pcr detection methods: one targeting the campylobacter jejuni hipo gene, one targeting the campylobacter coli glya ge ... | 2015 | 26187833 |
| restoring the selectivity of modified charcoal cefoperazone deoxycholate agar for the isolation of campylobacter species using tazobactam, a β-lactamase inhibitor. | extended spectrum β-lactamase (esbl) producing escherichia coli have emerged as a contaminant on modified charcoal cefoperazone deoxycholate agar (mccda) when attempting to selectively isolate campylobacter spp. from poultry. e. coli are particularly problematic given their ability to grow under microaerophilic conditions and have been shown to outcompete campylobacter species making campylobacter detection or enumeration difficult. this paper recommends a novel method for restoring the selectiv ... | 2015 | 26119190 |
| the evolution of campylobacter jejuni and campylobacter coli. | the global significance of campylobacter jejuni and campylobacter coli as gastrointestinal human pathogens has motivated numerous studies to characterize their population biology and evolution. these bacteria are a common component of the intestinal microbiota of numerous bird and mammal species and cause disease in humans, typically via consumption of contaminated meat products, especially poultry meat. sequence-based molecular typing methods, such as multilocus sequence typing (mlst) and whole ... | 2015 | 26101080 |
| comparative analysis of antimicrobial resistance and genetic diversity of campylobacter from broilers slaughtered in poland. | in the current study, the relationship of campylobacter jejuni and campylobacter coli strains isolated at slaughter was investigated using comparative analysis of antimicrobial resistance (amr), virulence gene (vg) and pfge profiling. a total of 254 campylobacter isolates from poultry caeca and corresponding carcasses, including 139 c. jejuni and 115 c. coli strains were tested. the most prevalent resistance profiles observed in c. jejuni were ciprofloxacin, nalidixic acid and tetracycline (46 o ... | 2015 | 26092707 |
| prevalence of campylobacter spp. in retail chicken, turkey, pork, and beef meat in poland between 2009 and 2013. | the purpose of the present study was to determine the prevalence of thermophilic campylobacter in poultry, pork, and beef meat at the retail level and to identify the main categories of meat representing the most significant reservoirs of campylobacter. a monitoring study was conducted throughout poland from 2009 to 2013. a total of 1,700 fresh meat samples were collected from supermarkets, large retail outlets, and smaller stores. thermophilic campylobacter species were detected in 690 (49.3%) ... | 2015 | 25951401 |
| a five-year study on prevalence and antimicrobial resistance of campylobacter from poultry carcasses in poland. | during 2009-2013 a total of 2114 swab samples collected from broiler carcasses in all 16 voivodeships (administrative districts) of poland were examined for the presence of campylobacter jejuni and campylobacter coli. the antimicrobial resistance of the isolates to ciprofloxacin, tetracycline and erythromycin using the mic method was also tested. it was found that 1151 (54.4%) carcasses were contaminated with campylobacter, with 50% of c. jejuni and c. coli species isolated from positive samples ... | 2015 | 25846926 |
| effect of exposure to stress conditions on propidium monoazide (pma)-qpcr based campylobacter enumeration in broiler carcass rinses. | campylobacter quantification by qpcr is unable to distinguish viable vs. dead cells in contrast to the culture-based iso 10272-2 reference method. propidium monoazide (pma) has been used to overcome this disadvantage. a campylobacter pma-qpcr enumeration method was evaluated for its consistency and compared to the culture-based enumeration for both artificially and natural contaminated broiler carcass rinses. the pma effect was further evaluated on stressed cells. five conditions, commonly encou ... | 2015 | 25791007 |
| higher resistance of campylobacter coli compared to campylobacter jejuni at chicken slaughterhouse. | in order to compare the prevalence of campylobacter coli and campylobacter jejuni during the processing of broilers at slaughterhouse a total of 848 samples were analyzed during 2012 in southern spain. four hundred and seventy six samples were collected from cloaca, carcass surfaces and quartered carcasses. moreover, 372 environmental swabs from equipment and scalding water were collected. minimum inhibitory concentration (mic) to ciprofloxacin, erythromycin, streptomycin, tetracycline and genta ... | 2015 | 25770597 |
| identification of campylobacter species isolates with phenotypic methods and polymerase chain reaction. | campylobacter jejuni (c. jejuni) and campylobacter coli (c. coli) are the most common bacterial causes of enterocolitis in humans. however, identification of the species level is not always possible using standard biochemical tests. | 2015 | 25731001 |
| survival after cryogenic freezing of campylobacter species in ground turkey patties treated with polyphosphates. | the use of polyphosphate-based marinades in the processing of poultry has been previously shown to increase the survival of campylobacter species present in the exudates derived from these products. this study investigates the effects that some of the same polyphosphates have on the survival of campylobacter species within a ground turkey product subjected to cryogenic freezing. ground turkey patties with two different polyphosphate formulations added in two different concentrations were artific ... | 2015 | 25710161 |
| [antimicrobial susceptibility and resistance mutations in campylobacter jejuni and c. coli isolates from human and meat sources]. | recently, there has been a marked increase in the number of reports of fluoroquinolone-resistant campylobacter jejuni and campylobacter coli. the aim of this study was to evaluate the prevalence of antimicrobial resistance and its genetic determinants in campylobacter species isolated from meat and human subjects in fukuoka prefecture, japan. between 2011 and 2013, 55 and 64 isolates were collected from meat (chicken meat and beef liver) and humans, respectively, in this prefecture. antimicrobia ... | 2015 | 26552121 |
| a receptor-binding protein of campylobacter jejuni bacteriophage nctc 12673 recognizes flagellin glycosylated with acetamidino-modified pseudaminic acid. | bacteriophage receptor-binding proteins (rbps) confer host specificity. we previously identified a putative rbp (gp047) from the campylobacter lytic phage nctc 12673 and demonstrated that gp047 has a broader host range than its parent phage. while nctc 12673 recognizes the capsular polysaccharide (cps) of a limited number of campylobacter jejuni isolates, gp047 binds to a majority of c. jejuni and related campylobacter coli strains. in this study, we demonstrate that gp047 also binds to acapsula ... | 2015 | 25354466 |
| free-living waterfowl as a source of zoonotic bacteria in a dense wild bird population area in northeastern spain. | salmonella spp. and campylobacter spp. are zoonotic bacteria that represent an economic and public health concern worldwide. due to the difficulty to collect samples from free-living waterfowl, little is known on their importance as a reservoir of zoonotic agents. thus, a study was conducted to determine the prevalence, genotypic diversity and antimicrobial susceptibility of salmonella and campylobacter from waterfowl in ebro delta (northeastern spain), a geographical area with a dense wild bird ... | 2015 | 24112278 |
| polycarbonate filtration technique is noninferior to mccda for isolation of campylobacter species from stool samples. | a total of 5963 diarrheic stool samples were cultivated for campylobacter spp. with use of modified charcoal cefoperazone deoxycholate agar (mccda) plates as well as a polycarbonate (pc) filter technique on blood agar plates. a total of 376 campylobacter jejuni/coli were isolated from both pc and mccda. six and three were isolated from pc and mccda only, respectively (p = ns). the pc technique is noninferior to mccda for isolation of c. jejuni/coli. | 2015 | 26022656 |
| the susceptibility of campylobacter concisus to the bactericidal effects of normal human serum. | campylobacter concisus is an emerging pathogen of the gastrointestinal tract that has been associated with barrett's oesophagus, enteritis and inflammatory bowel disease. despite having invasive potential in intestinal epithelial cells in-vitro, bacteraemic cases with c. concisus are extremely scarce, having only been reported once. therefore, we conducted a serum resistance assay to investigate the bactericidal effects of human complement on c. concisus in comparison to some other campylobacter ... | 2015 | 25627875 |
| novel gentamicin resistance genes in campylobacter isolated from humans and retail meats in the usa. | to understand the molecular epidemiology of gentamicin-resistant campylobacter and investigate aminoglycoside resistance mechanisms. | 2015 | 25645207 |
| rapid identification and classification of campylobacter spp. using laser optical scattering technology. | campylobacter jejuni and campylobacter coli are the two important species responsible for most of the campylobacter infections in humans. reliable isolation and detection of campylobacter spp. from food samples are challenging due to the interferences from complex food substances and the fastidious growth requirements of this organism. in this study, a novel biosensor-based detection called bardot (bacterial rapid detection using optical scattering technology) was developed for high-throughput s ... | 2015 | 25583335 |
| genotypes and antibiotic resistance of bovine campylobacter and their contribution to human campylobacteriosis. | campylobacter jejuni and campylobacter coli are the most important bacterial causes of human gastroenteritis. chicken has been recognized as a major source for human infection, whereas cattle might also contribute to a lesser extent. however, there is a paucity of information available regarding campylobacter in swiss cattle and their role for human campylobacteriosis. to gain more information on genotypes and antibiotic resistance of bovine c. jejuni and c. coli and on their contribution to hum ... | 2015 | 25511436 |
| antimicrobial activity of gallic acid against thermophilic campylobacter is strain specific and associated with a loss of calcium ions. | gallic acid has been suggested as a potential antimicrobial for the control of campylobacter but its effectiveness is poorly studied. the minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) of gallic acid against campylobacter jejuni (n = 8) and campylobacter coli (n = 4) strains was determined. gallic acid inhibited the growth of five c. jejuni strains and three c. coli strains (mic: 15.63-250 μg ml(-1)). gallic acid was only bactericidal to two c. coli strains ( ... | 2015 | 25475290 |
| prevalence, virulence, and antimicrobial resistance of campylobacter jejuni and campylobacter coli in white stork ciconia ciconia in poland. | the aim of this study was to investigate the role of white stork ciconia ciconia as a potential reservoir of campylobacter spp. antimicrobial resistance and the presence of putative virulence genes of the isolates were also examined. a total of 398 white stork chicks sampled in western poland in habitats with high density of breeding were examined. rectal swabs were collected during breeding season 2009-2012 from storks developing in a relatively pure environment (odra meadows), in polluted area ... | 2015 | 25456607 |
| characterization of campylobacter jejuni and campylobacter coli genotypes in poultry flocks by restriction fragment length polymorphism (rflp) analysis. | we describe a simple, rapid, and discriminatory methodology that allows the routine molecular characterization of campylobacter jejuni and campylobacter coli isolates. the proposed approach is built on one of the earliest and simplest molecular typing methods ever, consisting on the analysis of the fragments of different lengths generated by digestion of homologous dna sequences with specific restriction endonucleases, a process known as restriction fragment length polymorphism (rflp) analysis. ... | 2015 | 25399105 |
| effect of propionic acid on campylobacter jejuni attached to chicken skin during refrigerated storage. | the ability of propionic acid to reduce campylobacter jejuni on chicken legs was evaluated. chicken legs were inoculated with campylobacter jejuni. after dipping legs in either water (control), 1% or 2% propionic acid solution (vol/vol), they were stored at 4ºc for 8 days. changes in c. jejuni, psychrotrophs and pseudomonas counts were evaluated. washing in 2% propionic acid significantly reduced c. jejuni counts compared to control legs, with a decrease of about 1.62 log units after treatment. ... | 2015 | 27036744 |
| successful conservative management of campylobacter cholecystitis occurring post chemotherapy and rituximab: a rare disease entity. | campylobacter jejuni is commonly associated with gastroenteritis, but extremely few reports worldwide link it acute cholecystitis. these infectious complications can assume menacing proportions in the immunocompromised and need careful management. we present a report of such a case from australia, successfully managed conservatively, without surgery. | 2015 | 26913915 |
| [molecular mimicry and guillain-barré syndrome]. | one-thirds of patients develop guillain-barré syndrome subsequent to campylobacter jejuni enteritis. molecular mimicry exists between c. jejuni lipo-oligosaccharides and human peripheral nerve gangliosides gm1 and gd1a. igg antibodies against gm1 or gd1a are produced in one out of 5,000 patients with c. jejuni enteritis. the autoantibodies bind to gangliosides at the nodes of ranvier in the peripheral motor nerves and activate complement in situ. this is followed by the disappearance of the volt ... | 2015 | 26560949 |
| [clinical phenotypes in guillain-barré syndrome]. | guillain-barré syndrome can be classified into several phenotypes according to the type of predominantly disturbed nerve fibers, distribution of muscular weakness, and electrophysiological and pathological findings. although several regional variants including fisher syndrome and pharyngeal-cervical-brachial weakness were initially reported in western researchers, many labor-intensive studies by japanese researchers have significantly contributed to defining and highlighting these variants. this ... | 2015 | 26560946 |
| eric-pcr genotyping of some campylobacter jejuni isolates of chicken and human origin in egypt. | the public health importance of the genus campylobacter is attributed to several species causing diarrhea in consumers. poultry and their meat are considered the most important sources of human campylobacteriosis. in this study, 287 samples from chicken (131 cloacal swabs, 39 chicken skin, 78 chicken meat, and 39 cecal parts) obtained from retail outlets as well as 246 stool swabs from gastroenteritis patients were examined. a representative number of the biochemically identified campylobacter j ... | 2015 | 26579615 |
| conservation and covariance in small bacterial phosphoglycosyltransferases identify the functional catalytic core. | phosphoglycosyltransferases (pgts) catalyze the transfer of a c1'-phosphosugar from a soluble sugar nucleotide diphosphate to a polyprenol phosphate. these enzymes act at the membrane interface, forming the first membrane-associated intermediates in the biosynthesis of cell-surface glycans and glycoconjugates, including glycoproteins, glycolipids, and the peptidoglycan in bacteria. pgts vary greatly in both their membrane topologies and their substrate preferences. pgts, such as mray and weca, a ... | 2015 | 26600273 |
| [potential use of mass spectrometry for subtyping of campylobacter]. | molecular epidemiology is a field that uses results of typing techniques to obtain information on detailed characterization of bacterial strains for determining the identity, similarity or difference in bacteria of the same genus, species or serotype. nowadays, the most commonly used methods are based on monitoring differences in bacterial genotypes. however, most of these techniques are time-consuming and costly. a method increasingly used in routine microbiological testing is matrix-assisted l ... | 2015 | 26636631 |
| guillain barré syndrome (gbs): new insights in the molecular mimicry between c. jejuni and human peripheral nerve (hpn) proteins. | profile and immunoreactivity of proteins from hpn tissue, and from campylobacter jejuni (o:19) were investigated. proteins were extracted, separated by sds-page, their cross reactivity monitored by western blotting, and identified by nhplc-nesi-hrms analysis. proteins from c. jejuni, at mw ~70 kda were chaperone/co-chaperone proteins (groel, dnak and htpg). in the corresponding hpn band were serum albumin, neurofilament light peptide, cytoskeletal keratins and one hsp 70 and one hsp60. these cha ... | 2015 | 26616887 |
| pseudomonas aeruginosa facilitates campylobacter jejuni growth in biofilms under oxic flow conditions. | we investigated the growth of campylobacter jejuni in biofilms with pseudomonas aeruginosa under oxic flow conditions. we observed the growth of c. jejuni in mono-culture, deposited on pre-established p. aeruginosa biofilms, and co-inoculated with p. aeruginosa. in mono-culture, c. jejuni was unable to form biofilms. however, deposited c. jejuni continuously grew on pre-established p. aeruginosa biofilms for a period of 3 days. the growth of scattered c. jejuni clusters was strictly limited to t ... | 2015 | 26610432 |
| the campylobacter jejuni cprrs two-component regulatory system regulates aspects of the cell envelope. | 2015 | 26767725 | |
| [multilocus sequence typing of campylobacter jejuni isolates from guizhou province]. | 2015 | 26882572 | |
| loop-mediated isothermal amplification (lamp) for detection of campylobacter jejuni and c. coli in thai children with diarrhea. | campylobacter species are common causes of bacterial diarrhea, and campylobacter jejuni and c. coli are known as the predominant causative agents in humans. recent studies suggested that loop-mediated isothermal amplification (lamp) is an efficient and practical tool for rapid detection of c. jejuni and c. coli in clinical samples. we used lamp to screen 151 stool samples for campylobacter; these samples were collected in 2012 from thai children with diarrhea. the pcr method discriminated c. jej ... | 2015 | 25866115 |
| involvement of cholesterol in campylobacter jejuni cytolethal distending toxin-induced pathogenesis. | the aim of this study was to investigate whether cholesterol plays a pivotal role in cytolethal distending toxin (cdt) mediated pathogenic effects in hosts. | 2015 | 25865189 |
| application of pulsed field gel electrophoresis to type campylobacter jejuni. | pulsed field gel electrophoresis (pfge) is generally accepted as one of the most discriminatory methods available for genotyping campylobacter jejuni. pfge has been extensively used in epidemiological studies, including outbreak investigation, persistence of genotypes in a human population, environmental diversity of sporadic infection isolates, dissemination of antibiotic-resistant strains, and comparison of genotypes within and between hosts. the main purpose of this chapter is to present a wo ... | 2015 | 25862055 |
| an unconventional anaerobic membrane protein production system based on wolinella succinogenes. | in cases where membrane protein production attempts in more conventional escherichia coli-based systems have failed, a solution is to resort to a system based on the nonpathogenic epsilon-proteobacterium wolinella succinogenes. this approach has been demonstrated to be successful for structural and mechanistic analyses not only for homologous production of w. succinogenes membrane proteins but also for the heterologous production of membrane protein complexes from the human pathogens helicobacte ... | 2015 | 25857779 |
| expression of stress-related proteins in sediminibacterium sp. growing under planktonic conditions. | aggregation is a common trait of bacteria in natural and engineered biological systems. microbial aggregates, such as flocs, granules, and biofilms, are spatially heterogeneous environments. it is generally observed that by growing under aggregated conditions bacteria respond and adapt to environmental stress better than free-swimming bacteria of the same species. we performed a proteomic analysis of a strain of sediminibacterium, isolated from activated sludge, which grew planktonically in dilu ... | 2015 | 25847231 |
| the combined efficacy of carvacrol and modified atmosphere packaging on the survival of salmonella, campylobacter jejuni and lactic acid bacteria on turkey breast cutlets. | the primary objective of this study was to determine the efficacy of carvacrol in combination with modified atmosphere packaging (map) in reducing salmonella on turkey breast cutlets stored at 4 °c. in experiment i, carvacrol (0.5, 1, and 2% v/v) was applied as surface treatment and samples were stored under aerobic condition or as surface and dip treatments followed by storage in an environment of 100% carbon dioxide. the findings of the experiment i revealed the synergistic activity of carvacr ... | 2015 | 25846923 |
| effect of oxygen stress on growth and survival of clostridium perfringens, campylobacter jejuni, and listeria monocytogenes under different storage conditions. | this study investigated the growth and survival of three foodborne pathogens (clostridium perfringens, campylobacter jejuni, and listeria monocytogenes) in beef (7% fat) and nutrient broth under different oxygen levels. samples were tested under anoxic (<0.5%), microoxic (6 to 8%), and oxic (20%) conditions during storage at 7 °c for 14 days and at 22 °c for 5 days. two initial inoculum concentrations were used (1 and 2 log cfu per g of beef or per ml of broth). the results show that c. perfring ... | 2015 | 25836393 |
| campylobacter jejuni infection and ige sensitization in up to 2-year-old infants. | the "hygiene hypothesis" addresses the correlation between the occurrence of atopy and the frequency of infections in the earliest age, explaining an increase in the incidence of atopic diseases by living in good, infection-free, hygienic conditions. the aim of our study was to determine the conection between atopy and camplobacter infection, and to analyze the association between serum concentrations of total ige and campyobacter infection in relation to atopy in children up to two years. | 2015 | 25831906 |
| increased intracellular calcium level and impaired nutrient absorption are important pathogenicity traits in the chicken intestinal epithelium during campylobacter jejuni colonization. | although a high number of chickens carry campylobacter jejuni, the mechanistic action of colonization in the intestine is still poorly understood. the current study was therefore designed to investigate the effects of c. jejuni on glucose uptake, amino acids availability in digesta, and intracellular calcium [ca(2+)]i signaling in the intestines of broiler chickens. for this, we compared: control birds (n = 60) and c. jejuni-infected birds (n = 60; infected orally with 1 × 10(8) cfu of c. jejuni ... | 2015 | 25825050 |
| cytochrome c biogenesis in campylobacter jejuni requires cytochrome c6 (ccca; cj1153) to maintain apocytochrome cysteine thiols in a reduced state for haem attachment. | the microaerophilic food-borne pathogen campylobacter jejuni uses complex cytochrome-rich respiratory chains for growth and host colonisation. cytochrome c biogenesis requires haem ligation to reduced apocytochrome cysteines, catalysed by the cytochrome c synthase, ccsba. while ccsba could not be deleted, we showed that the thiol reductase dsbd and the ccsx homologue cj1207 are involved in, but not essential for, cytochromes c biogenesis. mutant phenotypic analyses and biochemical studies with p ... | 2015 | 25825009 |
| antimicrobial activity and stability of weakly acidified chlorous acid water. | the antimicrobial activity of weakly acidified chlorous acid water (wacaw) against staphylococcus aureus, non-pathogenic escherichia coli, enterohemorrhagic e. coli (ehec o157:h7), candida albicans, and spore-forming bacillus and paenibacillus species was evaluated in vitro. the antiviral activity was also examined using feline calicivirus (fcv). diluted wacaw (>100 ppm) effectively reduced the number of non-spore-forming bacteria (>4 log10 cfu reductions) within 5 min. treatment with this sanit ... | 2015 | 25817812 |
| prevalence and characterization of campylobacter jejuni from chicken meat sold in french retail outlets. | campylobacter was detected in 76% of broiler meat products collected in retail outlets during a monitoring plan carried out in france throughout 2009. campylobacter jejuni was the most prevalent species (64.7% of products being contaminated). the 175 c. jejuni isolates collected were characterized. mlst typing results confirmed substantial genetic diversity as the 175 c. jejuni isolates generated 76 sequence types (sts). the st-21, st-45 and st-464 complexes predominated accounting for 43% of al ... | 2015 | 25770428 |
| role of capsular modified heptose in the virulence of campylobacter jejuni. | the campylobacter jejuni capsular polysaccharide is important for virulence and often contains a modified heptose. in strain atcc 700819 (a.k.a. nctc 11168), the modified heptose branches off from the capsular backbone and is directly exposed to the environment. we reported previously that the enzymes encoded by wcag, mlghb and mlghc are involved in heptose modification. here, we show that inactivation of any of these genes leads to production of capsule lacking modified heptose and alters the t ... | 2015 | 25766665 |
| identification of campylobacter jejuni and determination of point mutations associated with macrolide resistance using a multiplex taqman mgb real-time pcr. | the aim of the study was to develop a multiplex real-time pcr method to identify campylobacter jejuni containing mutations commonly associated with macrolide resistance. | 2015 | 25766481 |
| antibiotic susceptibility profiling and virulence potential of campylobacter jejuni isolates from different sources in pakistan. | to determine antibiotic resistance patterns and virulence potential of campylobacter jejuni (c. jejuni) isolates from clinical human diarrheal infections, cattle and healthy broilers. | 2015 | 25902160 |
| molecular survey of occurrence and quantity of legionella spp., mycobacterium spp., pseudomonas aeruginosa and amoeba hosts in municipal drinking water storage tank sediments. | to examine the occurrence and quantity of potential pathogens and an indicator of microbial contamination in the sediments of municipal drinking water storage tanks (mdwsts), given the absence of such data across the united states. | 2015 | 25891145 |
| notes from the field: campylobacteriosis outbreak associated with consuming undercooked chicken liver pâté - ohio and oregon, december 2013-january 2014. | on january 8, 2014, the ohio department of health notified the oregon public health division (ophd) of campylobacteriosis in two ohio residents recently returned from oregon. the travelers reported consuming chicken liver pâté* at an oregon restaurant. on january 10, ophd received additional reports of campylobacteriosis in two persons who had consumed chicken liver pâté at another oregon restaurant. campylobacter jejuni was isolated in cultures of fecal specimens from three patients. ophd inves ... | 2015 | 25879900 |
| [effects of the environment on health of feral pigeons (columba livia)]. | we examined 80 feral pigeons and their fecal samples from two feral pigeon lofts of the "pigeon action of basel" (switzerland) for different pathogens. the tested material harbored four pathogenic agents transmissible to humans (chlamydia spp., salmonella spec., campylobacter jejuni, cryptococcus neoformans) in addition several pathogens were found which are no zoonotic agents but potentially pathogenic for the pigeons themselves, such as trichomonas gallinae, coccidia, helminths, ectoparasites ... | 2015 | 25876285 |
| the signalling molecule autoinducer-2 is not internalised in campylobacter jejuni. | bacteria recognise and constantly adjust to changing situations by sensing environmental and self-produced signals. autoinducer-2 (ai-2) is a signal molecule, found in many bacterial species and thus proposed to enable interspecies com- munication. two classes of ai-2 receptors have been identified so far. one class of ai-2 receptors sense ai-2 by a two component signalling system.the other class, the lsrb family, internalises and phosphorylates ai-2. campylobacter (c.) jejuni is known to produc ... | 2015 | 25876270 |
| changes within the intestinal flora of broilers by colonisation with campylobacter jejuni. | in most european countries human campylobacteriosis is the most important bacterial zoonotic foodborne infection. chicken meat is considered the main source of infection. since most strategies assessed so far, in reducing campylobacter colonization in chickens or in the reduction of human disease, have not been very effective, new knowledge regarding campylobacter's interaction with the host is needed. in this study we analysed fecal and cecal samples of five chicken flocks of different austrian ... | 2015 | 25876269 |
| murine infection models for the investigation of campylobacter jejuni--host interactions and pathogenicity. | despite the socioeconomic burden of campylobacteriosis, our insights into the molecular mechanisms underlying campylobacter (c.) jejuni-induced intestinal immunopathogenesis are limited.the absence and the ban of convenient murine infection models caused fundamental restrictions in campylobacter research. the development of novel and modified murine infection models in the last years has greatly contributed to our knowledge in cjejuni--host interactions and pathogenicity. novel findings revealed ... | 2015 | 25876268 |
| campylobacter jejuni: components for adherence to and invasion of eukaryotic cells. | campylobacter (c.) jejuni is the most important reported cause for bacterial diarrhoea. the infection can be accompanied by fever and abdominal cramps and in rare cases the guillain-barré syndrome or reactive arthritis can develop as a post-infection complication. several biological properties of cjejuni, e. g. motility and chemotaxis, contribute to the biological fitness of the pathogen. for this, deficiencies in the function of these features clearly reduce the pathogenicity of c. jejuni witho ... | 2015 | 25876267 |
| claudin-related intestinal diseases. | with up to 200 m(2) the human intestine is the organ with the largest absorptive surface of the body. it is lined by a single layer of epithelial cells that separates the host from the environment. the intestinal epithelium provides both, selective absorption of nutrients, ions, and water but also a highly effective barrier function which includes the first line of defense against environmental antigens. the paracellular part of this barrier function is provided by tight junction (tj) proteins, ... | 2015 | 25999319 |
| campylobacter jejuni contamination of broiler carcasses: population dynamics and genetic profiles at slaughterhouse level. | six slaughter batches deriving from six typical industrial broiler flocks were examined for the presence, quantity and genetic characteristics of contaminating campylobacter jejuni (c. jejuni) during various stages of slaughtering and carcass processing. to assess the contamination dynamics of the carcasses, the analyses were always conducted on neck-skin samples from the same pre-selected and carefully marked carcasses in each batch. the skin samples were taken sequentially at three successive ... | 2015 | 25998821 |
| perr controls oxidative stress defence and aerotolerance but not motility-associated phenotypes of campylobacter jejuni. | the foodborne bacterial pathogen campylobacter jejuni is an obligate microaerophile that is exposed to atmospheric oxygen during transmission through the food chain. survival under aerobic conditions requires the concerted control of oxidative stress systems, which in c. jejuni are intimately connected with iron metabolism via the perr and fur regulatory proteins. here, we have characterized the roles of c. jejuni perr in oxidative stress and motility phenotypes, and its regulon at the level of ... | 2015 | 25968890 |
| genetic diversity and host associations in campylobacter jejuni from human cases and broilers in 2000 and 2008. | campylobacter jejuni is an important food-borne pathogen, with a global distribution. it can colonize numerous host species, including both domestic and wild animals, but is particularly associated with birds (poultry and wild birds). for human campylobacteriosis, poultry products are deemed the most significant risk factor for acquiring infection. we conducted a genotyping and host attribution study of a large representative collection of c. jejuni isolated from humans and broilers in sweden in ... | 2015 | 25960333 |
| studies on tridecaptin b(1), a lipopeptide with activity against multidrug resistant gram-negative bacteria. | previously other groups had reported that paenibacillus polymyxa nrrl b-30507 produces srcam 37, a type iia bacteriocin with antimicrobial activity against campylobacter jejuni. genome sequencing and isolation of antimicrobial compounds from this p. polymyxa strain show that the antimicrobial activity is due to polymyxins and tridecaptin b1. the complete structural assignment, synthesis, and antimicrobial profile of tridecaptin b1 is reported, as well as the putative gene cluster responsible for ... | 2015 | 25959079 |
| reactive arthritis in relation to internal derangements of the temporomandibular joint: a case control study. | the aim of this study was to find out if reactive arthritis was involved in the aetiology of chronic closed lock of the temporomandibular joint (tmj) by looking for bacterial antigens in the synovial membrane of the tmj, and by studying the antibody serology and carriage of human leucocyte antigen (hla) b27 in patients with chronic closed lock. patients with reciprocal clicking and healthy subjects acted as controls. we studied a total of 43 consecutive patients, 15 with chronic closed lock, 13 ... | 2015 | 25957137 |
| impact of disinfectant wipes on the risk of campylobacter jejuni infection during raw chicken preparation in domestic kitchens. | in the present study, we conducted a quantitative microbial risk assessment forecasting the exposure to campylobacter jejuni contaminated surfaces during preparation of chicken fillets and how using a disinfectant-wipe intervention to clean a contaminated work area decreases the risk of infection following the preparation of raw chicken fillet in a domestic kitchen. | 2015 | 25939813 |
| microbial food safety: potential of dna extraction methods for use in diagnostic metagenomics. | the efficiency of ten widely applied dna extraction protocols was evaluated for suitability for diagnostic metagenomics. the protocols were selected based on a thorough literature study. chicken fecal samples inoculated with about 1×10(3) and 1×10(6) cfu/g campylobacter jejuni were used as a model. the evaluation was performed based on total dna yield measured by fluorometry, and quality and quantity of c. jejuni dna measured by real-time pcr. there was up to a 25-fold variance between the lowes ... | 2015 | 25937085 |