Publications

[the determination of neuraminidases in vibrios of the 01 and non-01 groups].	vibrio cholerae 01 and non 01 neuraminidase may be rapidly detected with the use of a synthetic 4-methylumbelliferyl-neuraminic acid substrate. other vibrios and enterobacteriaceae show no neuraminidase activity against this substrate. the method may be used with microorganism cultures grown in solid and liquid nutrient media. the time of analysis is 15-20 min.	1990	1705608
[optimization of bacteriologic studies of cholera].	a colored medium is described, consisting of dry nutrient agar, 1 percent mannose, and 0.02 percent bromothymol blue. use of this medium helps accelerate the process of vibrio growth and differentiate mannose-positive vibrios from mannose-negative ones. the described medium is simple to make and use, thus recommending it for bacteriologic laboratories.	1990	1705614
microbial isopenicillin n synthase genes: structure, function, diversity and evolution.	clinically and economically, penicillins and cephalosporins are the most important class of the beta-lactam antibiotics. they are produced by a wide variety of microorganisms including numerous species of streptomyces, some unicellular bacteria and several filamentous fungi. a key step common to their biosynthetic pathways is the conversion of a linear, cysteine-containing tripeptide to a bicyclic beta-lactam antibiotic by isopenicillin n synthase. recent successes in the cloning and expression ...	1990	1366527
production of cholera toxin subunit b by a mutant strain of vibrio cholerae.	the b subunit (ctb) of cholera toxin (ct) can be used as a carrier protein for conjugate vaccines designed to elicit antipolysaccharide antibodies. a defined medium, agm4, was designed to grow a high-producing mutant of vibrio cholerae expressing only the b subunit of ct: v. cholerae 0395-ni. agm4 contains four amino acids, asparagine, glutamic acid, arginine and serine, salts and a trace element solution. the carbon source is glucose. the fermentations performed in agm4 indicated that ctb produ ...	1990	1366742
antibacterial activity of clove extracts against phagogenic strains including clinically resistant isolates of shigella and vibrio cholerae.	clove extracts (petroleum ether, chloroform and ethanol extracts) were tested in vitro for their antibacterial activity against forty isolates of pathogenic bacteria including clinically resistant (resistant to ampicillin and nalidixic acid) strains of twenty-five shigella and four vibrio cholerae. all of the isolates except pseudomonas aeruginosa showed promising sensitivity to the extracts.	1990	16414655
erratum: variation in epitopes of the b subunit of vibrio cholerae non-o1 and vibrio mimicus cholera toxins.		1990	22049941
[acute lymphoblastic leukemia and lymphoblastic lymphoma. two names for one disease (author's transl)].	of 14 non-t-cell malignant non-hodgkin lymphomas (mnhl) 5/14 presented with leukemic transformation of the bone marrow; in three more cases bone marrow involvement developed during an observation period of 60--200weeks. in 2/14 cases mnhl terminated the first continuous and complete remission of acute lymphoblastic leukemia (all). cns involvement has been observed without preceding bone marrow involvement. only 4/14 mnhl remained local diseases. these clinical courses as well as hematological fi ...	1990	267804
a novel autoregulatory cytokine is required for the regulation of autoaggressive responses.	limiting dilution studies indicate that cells with the potential to lyse autologous target cells exist in the peripheral blood of all normal individuals. in contrast to allocytotoxic cells, autocytolytic cells are down-regulated by a second less frequent cell population. when recombinant interleukin 2 is substituted for crude lymphocyte conditioned medium in these limiting dilution experiments, autocytotoxicity develops normally. under these conditions, however, the autocytotoxic response is not ...	1990	2187837
the relevance of 25-hydroxycalciferol measurements in sera of patients with renal failure.	iliac crest biopsies and serum specimens were obtained from 36 non-dialyzed uraemic patients. the mean serum 25-hydroxycalciferol concentration of the patients was lower than that of normal subjects and a significant correlation was found between 25-hydroxycalciferol values and the severity of osteomalacia. parathyroid osteopathy was significantly correlated with serum immunoreactive parathyroid hormone but not with serum 25-hydroxycalciferol values. while normal serum 25-hydroxycalciferol conce ...	1990	313783
vestibular effects of antibiotics introduced in the inner ear. behavioral and electrophysiological studies in the frog.	the aim of this study was to find a rapid and suitable method for testing the toxicity of drugs upon the vestibular end organs. the experiments were performed in the frog and the action of two antibiotics, streptomycin sulphate and penicillin g, was studied by carefully observing the equilibrium behaviour of the animals and recording the spontaneous activity of the nerve of the horizontal semicircular canal. the results obtained show that: 1) injection of streptomycin sulphate (20 microgram) dir ...	1990	313657
exercise tests in male diabetics. a nine years' follow-up study with special reference to ecg changes and cardiovascular morbidity.		1990	268141
cystosarcoma phyllodes of the breast.		1990	184742
clinical aspects of grief and bereavement.		1990	258819
endophthalmitis after staphylococcal sepsis.		1990	313769
aortic valve replacement in a patient with von willebrand's disease.	few cases have been reported to date of cardiac surgery performed on patients with von willebrand's disease. this report details the management of such a patient, who underwent aortic valve replacement for severe calcific aortic stenosis.	1990	313787
action of adenosine on chloride active transport of isolated frog cornea.	addition of adenosine (10-7 to 10-4 m) to the tear side of isolated corneas (rana catesbeiana) produced a rapid, sustained increase in short-circuit current, potential difference, and radioisotopic chloride net flux. the increased net chloride flux accounted for the increased short-circuit current. adenosine, a known activator of adenyl cyclase in other tissues, exerted its effects on chloride transport through a receptor different from the one described for epinephrine and prostaglandins in the ...	1990	313716
differentiation of b cells and the clonal abortion theory.		1990	313669
[systemic lupus erythematosus (s.l.e.) with pregnancy].		1990	313663
[diagnostic significance of the occurrence of tissue antigen hla-b27 in various rheumatic diseases].		1990	313621
tests and management affecting survival after portacaval and spenorenal shunts.	new data were derived from records of 115 patients undergoing portacaval or spenorenal shunts from 1966 to 1973. the last serum bilirubin level determined before a shunt was the best predictor of operative mortality. the strongest predictor of long term survival was the first serum albumin level measured after admission. operative mortality was strongly correlated with intraoperative clotting disorders; however, the prothrombin time and the partial thromboplastin time predicated long term surviv ...	1990	313608
[from a study in molde: positive experiences with active learning].		1990	258880
emphasis placed on winning in athletics by male high school students.		1990	267961
[an attempt to improve the contents of nursing records - based on the survey of nurse's image].		1990	258694
[my experience as a patient - on a simple standard of values].		1990	258689
[clinical and statistical study of 100 vitiligo patients. i. etiologic factors and clinical study].	a study of the etiological factors in 100 cases of vitiligo showed female predominance (59%) variable age of onset, absence of radial factor, moderate influence of psychological, physical or endocrine factors, the large number of subjects belonging to group b, a family history in 18% of cases. the morphology site and course of the lesions were also studied. there was no significant correlation between the course of the disease and the various etiological and clinical parameters studied.	1990	184543
neurophysiological defects in temperature-sensitive paralytic mutants of drosophila melanogaster.	a new temperature-sensitive paralytic mutant of drosophila, comatose, is compared behaviorally and physiologically with the previously known types, para and shi. all three have different properties with respect to kinetics of paralysis at high temperature and recovery from paralysis; com is hypersensitive to paralysis by cooling. neurophysiological experimeents indicate different mechanisms for paralysis in each of the mutants.	1990	184469
single health agency: viable concept.		1990	267827
transplantation shock: a geriatric relocation.		1990	258653
[comparative study of spirographic values and scintigraphic aspects in patients with bronchopulmonary diseases].	a correlation was made of routine spyrographic data with the scintigraphic aspects of the lungs in 146, respectively 100 patients with bronchopulmonary diseases. the following conclusions can be inferred: 1) normal spirographic values do not necessarily reflect an integrity of the respiratory function. besides these values pulmonary perfusion can be severily altered; 2) the more deteriorated the spirographic values the more probable will be the alteration of the pulmonary perfusion; 3) in the ev ...	1990	171728
painless subacute thyroiditis: a report of two cases.		1990	267808
[lymphographic investigation of the face-neck-region (author's transl)].	indirect lymphography is shown to be a technically uncomplicated and easy to use method of displaying the lymph nodes and -vessels of the face and neck regions. "lipiodol-ultra-fluid" an oily contrast medium which has used in experiments with minipigs can be radiologically and histologically demonstrated in nodes for months. the histological findings in the course of a year are, at the beginning a phase of non-specific reaction followed by a phase of granulomatous reaction between two weeks and ...	1990	184356
[internal porosity in composite resins].		1990	296485
temporomandibular dysfunction in pretreatment adolescent patients.		1990	269671
pars plana vitrectomy in auckland.		1990	269552
don't expect - at her age!		1990	258408
[reconstruction of the bony orbital ring by means of mini-dc-plates after mid-facial fractures].	surface and structure of austenitic steel wire are changed when processed. demonstration of unused wire, wire during processing as well as wire used as implant in fracture healing after its removal. examination of metal ground sections of the used wire did not show any changes of the crystal structure in the sense of corrosion.	1989	267009
[infectious risk in ambulatory treatments with cutaneous breaking].		1989	1836607
idiotype-binding cells in plasmacytoma-bearing mice.		1989	313254
the effects of recurrent inhibition on the cross-correlated firing patterns of motoneurones (and their relation to signal transmission in the spinal cord-muscle channel).		1989	209834
cytochrome c peroxidase catalyzed oxidations of substitution inert iron(ii) complexes.		1989	184138
hansen's disease; wanted: more than just clinical nursing.		1989	4485999
neovascularization of surface demineralized dentin.	uneventful healing of the wound site created by periodontal reconstructive surgery is crucial for the long term survival of the dentition. wound healing has been shown to be initiated and mediated by matrix components and polypeptide growth factors. neovascularization (or angiogenesis) is one of the most important events in the healing process of a wound site. any increase in the degree and/or rate of neovascularization could result in more rapid or complete healing. previously, we have shown th ...	1989	2481025
[cytogenetic and cytological approaches to detection of gamete abnormalities (review of the literature)].		1989	2481072
[changes in cerebral level of monoamines by japanese encephalitis virus infection].	the changes in monoamine levels of different brain regions following japanese encephalitis virus (jev) intraperitoneal inoculation were examined in experimentally jev-infected mice. in addition, virus distribution was studied using infectivity assay and immuno-histochemistry of viral antigen. 1) the level of monoamines in brain tissues was not affected by 48 hours after viral inoculation, but marked effects were elicited at 96 hours after the inoculation. the cerebral concentration of 5-hydroxyi ...	1989	2481370
separation of branched sialylated oligosaccharides using high-ph anion-exchange chromatography with pulsed amperometric detection.	ten characterized sialylated oligosaccharides from bovine fetuin (b. bendiak, m. harris-brandts, s. w. michnick, j. p. carver, and d. a. cumming, biochemistry, in press; and d. a. cumming, c. g. hellerqvist, m. harris-brandts, s. w. michnick, j. p. carver, and b. bendiak, biochemistry, in press) were chromatographed using high-performance anion-exchange chromatography with pulsed amperometric detection. at near neutral ph values, oligosaccharides were separated according to their number of forma ...	1989	2481411
relationship between structure and antigenicity of o1 vibrio cholerae lipopolysaccharides.	the relationship between the release of fructose from o1 vibrio cholerae lipopolysaccharides (lps) by dilute acetic acid hydrolysis and the decrease in their antigenicity was examined. decrease in the antigenicity of lps was not parallel with the release of fructose, and occurred very much later than the latter. periodate oxidation of lps resulted in the total elimination of the fructose and glucose, and two-thirds of the heptose constituents, but no difference in the antigenicity of lps was obs ...	1989	2482336
variation in epitopes of the b subunit of el tor and classical biotype vibrio cholerae o1 cholera toxin.	variation in epitopes of the b subunit of cholera toxin (ct-b) produced by strains of el tor and classical biotype vibrio cholerae o1 was examined using monoclonal antibodies prepared to v. cholerae 569b ct. ct-b epitopes were markedly conserved for v. cholerae classical biotypes. in contrast, epitope variation was observed for el tor biotypes, which produced both a classical-like ct-b and a unique ct-b lacking at least one epitope common to 569b ct-b. the missing epitope was located outside the ...	1989	2482860
occurrence and characteristics of agglutination of vibrio cholerae by serum from the eastern oyster, crassostrea virginica.	cell-free hemolymph (serum) of the eastern oyster, crassostrea virginica, agglutinated vibrio cholerae, including all o1 serovars and biovars. seventy-nine other strains of bacteria, including 14 genera and 26 species, were not agglutinated. the a, b, and c factors of o1 antigen were not involved in agglutination. bacterial agglutinating (ba) activity was demonstrated for oysters inhabiting different environments of the u.s. atlantic and gulf coasts. oyster serum ba titers showed high individual ...	1989	2483041
[current problems in the microbiological control of food].	the paper deals with a number of aspects of current interest and significance on the subject of the microbiological testing of food with particular reference to the role of research institutes. the main objectives of microbiological testing of food are essentially two: (i) to establish the absence of a human health hazard due to microbial contamination of food (food safety); and (ii) to define the quality standard of food (food quality). as far as the first of these objectives is concerned, the ...	1989	2483651
[the fate of pathogens in waste-waters].	a large variety of pathogenic organisms capable of transmission by the faecal-oral route may be found in wastewaters. among the bacteria salmonella, shigella, vibrio cholerae, yersinia and campylobacter are the important agents of concern. also the human enteric viruses (poliovirus, coxsackievirus, echovirus, hepatitis a virus, rotavirus) have been shown to be present in domestic waste and may not be completely removed by conventional sewage treatment processes, including chlorination. discharge ...	1989	2484470
a bacteriological study of cholera in gulbarga district.		1989	2486988
immunocytochemical study of localization and traffic of thyroid peroxidase/microsomal antigen.	we studied the distribution of binding sites for anti-peroxidase monoclonal antibody and anti-microsomal antibodies on isolated human thyroid follicles and a human thyroid cell line. both open follicles and cells were incubated first with antibodies at +4 degrees c, then with colloidal gold labelled protein a. the topography of the binding sites for monoclonal anti-peroxidase antibody corresponded closely to the expected cell surface distribution of endogenous thyroid peroxidase since labelling ...	1989	2491623
toxigenic vibrio cholerae o1 infection acquired in colorado.		1989	2491910
recombinant system for overexpression of cholera toxin b subunit in vibrio cholerae as a basis for vaccine development.	we have constructed an overexpression system in which the gene encoding the b subunit of cholera toxin (ctb) was placed under the control of the strong tacp promoter in a wide host range plasmid. recombinant nontoxigenic classical and e1 tor vibrio cholerae strains of different serotypes harboring this plasmid excreted 10- to 100-fold higher amounts of ctb than any other wild-type or recombinant strain tested and may therefore be useful killed oral vaccine strains. the manipulations to place the ...	1989	2492108
immunobiological relationships among new cholera toxins produced by ct gene-negative strains of vibrio cholerae o1.	the enterotoxic activities of partially purified new cholera toxins (nct) prepared from the ct gene-negative strains of vibrio cholerae serotype o1 isolated from cases of diarrhoea in man and from diverse environmental sources were assayed in the rabbit ileal-loop model and the toxin unit was calculated in micrograms of protein content. the secretory activities of one unit of homologous and heterologous enterotoxins were completely neutralised by 2.5 units of crude antiserum raised against one n ...	1989	2492345
lack of effect by prostaglandin f2 alpha and verapamil on calcium uptake by isolated corpora lutea from pseudopregnant rats.		1989	2492809
a review of experimental studies of iodine deficiency during fetal development.	iodine deficiency is now recognized as a major international public health problem. it is estimated that 800 million people may be at risk of the effects of iodine deficiency. in humans, the effects occur at all stages of development: the fetus, the neonate, the child and adult. the effects are now denoted by the term iodine deficiency disorders (idd). they include miscarriages, stillbirths, congenital anomalies, as well as the more familiar goiter, cretinism, impaired brain function, and hypoth ...	1989	2493080
experimental transmission of crimean-congo hemorrhagic fever virus by hyalomma truncatum koch.	larval hyalomma truncatum ticks were infected with crimean-congo hemorrhagic fever (cchf) virus by allowing them to engorge on viremic newborn mice. the overall tick infection rate was 4.4% (24/542). virus was detected in specimens for greater than or equal to 160 days postinfection. transstadial transmission to the adult tick stage was observed and horizontal transmission to a mammalian host was demonstrated. horizontal transmission of cchf virus to uninfected adult ticks occurred while feeding ...	1989	2493203
monitoring of intestinal transplant rejection.	our results show that maltase, sucrase, and lactase activity are present at a normal level in nonrejecting small bowel transplants after an initial postoperative decline. this confirms that the disaccharide absorbing capacity of these grafts is intact. in allogeneic bowel, however, the levels of maltase and sucrase decline as histologic rejection occurs. these results suggest that serial maltase, sucrase, and possibly lactase levels in allogeneic intestinal transplants may serve as a useful adju ...	1989	2493205
in vitro susceptibility of feline herpesvirus-1 to vidarabine, idoxuridine, trifluridine, acyclovir, or bromovinyldeoxyuridine.	in vitro activities of 9-[( 2-hydroxyethoxy] methyl) guanine (acyclovir), (e)-5-(2-bromovinyl)-2'deoxyuridine, 9-beta-d-arabinofuranosyladenine (vidarabine), 5-iodo-2'-deoxyuridine (idoxuridine), and 5-trifluoromethyl-2'-deoxyuridine (trifluridine) were studied against 6 strains of feline herpesvirus-1. a significant difference was not detected among viral strains in their susceptibility to these compounds (p = 0.442). the relative potency of these compounds was trifluridine much greater than id ...	1989	2493210
structural studies of the asparagine-linked sugar chains of two immunoglobulin m's purified from a patient with waldenström's macroglobulinemia.	the structures of the sugar chains present in two human monoclonal igm molecules purified from the serum of a patient with waldenström's macroglobulinemia have been determined. the asparagine-linked sugar chains were liberated as oligosaccharides by hydrazinolysis and labeled by reduction with nab3h4 after n-acetylation. their structures were studied by serial lectin column chromatography and sequential exoglycosidase digestion in combination with methylation analysis. these two igm's were shown ...	1989	2493215
analysis of amikacin-resistant pseudomonas aeruginosa developing in patients receiving amikacin.	during a 36-month period, 28 patients treated for infections due to amikacin-susceptible pseudomonas aeruginosa subsequently developed infections or colonization with amikacin-resistant p aeruginosa at the same site. eleven amikacin-susceptible/-resistant pairs of isolates were analyzed for aminoglycoside-inactivating enzymes, plasmid profiles, cellular proteins, outer membrane proteins (omps), lipopolysaccharide (lps) profiles, and amikacin uptake. while clearly distinct from isolates of other ...	1989	2493230
inhibition of ca2+-induced cytosolic enzyme efflux from skeletal muscle by vitamin e and related compounds.	1. efflux of an intracellular enzyme (creatine kinase) from normal rat skeletal muscles was induced by treatment with the ca2+ ionophore a23187. addition of alpha-tocopherol (230 microm) to the incubation medium was found to significantly diminish this efflux, and this effect was mimicked by alpha-tocopherol acetate, phytol and isophytol, but not by trolox c (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid). 2. analysis of muscle cation content has shown that these protective effects of a ...	1989	2493242
purification of high and low molecular weight plasminogen activator inhibitor 1 from fibrosarcoma cell-line ht 1080 conditioned medium.	functionally active (high-mr) and inactive (low-mr) plasminogen activator inhibitor 1 (pai) have been purified from fibrosarcoma cell-line ht 1080 conditioned medium, containing 1% fetal calf serum. the two forms were first purified by affinity chromatography on heparin-sepharose and then separated from each other by gel filtration on sephadex g-150. the final purification was achieved by affinity chromatography on insolubilized monoclonal antibodies towards human pai. alternatively, the low-mr ...	1989	2493266
[soy lipoxygenase as the object for the primary testing of potential inhibitors of leukotriene biosynthesis].	the comparative study of the ability of three compounds (indomethacin, agent bw 755 c and arachidonate hydroxamic acid) to inhibit the activity of preparations of soya bean lipoxygenase and to block leukotriene c4 biosynthesis in human leukocytes recorded by reversed phase liquid chromatography was performed. it was shown that irrespectively of purification degree soya bean lipoxygenase is a sufficiently adequate object for primary testing of inhibitors of biosynthesis of leukotrienes with the g ...	1989	2501105
"new" food-borne pathogens of public health significance.	recent work by epidemiologists and microbiologists has uncovered several hitherto unrecognized food-borne bacterial pathogens of public health significance. one of these, listeria monocytogenes, has attracted considerable attention because of two major cheese-related outbreaks of listeriosis that were characterized by cases of meningitis, abortion, and perinatal septicemia. thus far, l. monocytogenes has been responsible for well over 300 reported cases of food-borne listeriosis, including about ...	1989	2501376
effects of salinity on the survival of non-o1 vibrio cholerae under environments of low temperature.	under low temperature conditions, the effects of survival and growth of non-o1 vibrio cholerae were studied in laboratory microcosms. as the environmental factors, various concentrations of nacl solution and that of mono- and bivalent cation added nutrient of polypeptone were used under several temperatures. the results of these experiments suggested an extended survival of non-o1 v. cholerae at low temperature. when a range of nacl concentration in microcosms water was between 0.4% and 2.0% wit ...	1989	2501431
oral rehydration formula containing alanine and glucose for treatment of diarrhoea: a controlled trial.	to determine whether adding l-alanine to the glucose based oral rehydration solution recommended by the world health organisation would improve its efficacy in treating acute diarrhoea.	1989	2502251
the immunodiagnosis of schistosoma mansoni infection in the different clinico-parasitological stages.	a total of 52 bilharzial mansoni patients were examined, 20 patients with early intestinal infection with living ova in stools, 20 patients with hepatosplenomegaly but without ascites, also with viable ova in stools, and 12 patients with hepatosplenomegaly and ascites without ova in stools; but in the rectal snip. also 20 normal persons were examined. an idt test was performed by s. mansoni adult worm antigen. s. mansoni adult worm and egg antigen extracts were used for performing ciep, ihat and ...	1989	2504828
assay of heat-labile enterotoxins by their adp-ribosyltransferase activities.	simple enzymatic assays to detect heat-labile enterotoxins whose modes of action are similar to that of cholera toxin were evaluated. the assays are performed by using an artificial substrate, diethylamino benzylidine-aminoguanidine, which is an adp-ribose acceptor. the product, formed in the presence of nad+, can be quantitated by spectrofluorometric, spectrophotometric, or high-performance liquid chromatographic (hplc) methods. as little as 25 ng (spectrofluorometry) or 125 ng (spectrophotomet ...	1989	2509510
[increased efficiency in the disinfection of sewage by lactic acid bacteria].	the antimicrobic properties have been studied in 30 strains of lactobacilli. as a result a strain, the strongest antagonist relative to choleric vibrios and other enteropathogenic microorganisms, is selected. lactobacilli are found to retain their viability and biological activity in the activated sludge during the whole period of observation (6 months). biological disinfection of sewage is shown possible to be intensified using the activated sludge inoculated by the culture of the selected lact ...	1989	2514340
an outbreak of nosocomial cholera in a 755-bed hospital.	from 30 october to 7 december 1984, an outbreak of nosocomial cholera involving 11 cases of biotype el tor, serotype inaba, took place in a 755-bed hospital in southern thailand. the outbreak occurred primarily among patients admitted with severe illness. of the 11 cases, 7 were children and 4 were adults. most cases had mild symptoms of cholera and no case died in this outbreak. the first 2 cases occurred sporadically with a subsequent cluster of cases showing an explosive pattern. a case-contr ...	1989	2514477
an immunocytochemical study of the proliferating cell nuclear matrix antigen p125/6.5 during rat spermatogenesis.	in previous studies of proliferating mammalian cells a p125/6.5 nuclear matrix antigen displaying a marked increase in mitotic cells has been identified. this antigen was investigated by immunocytochemistry of cryosections of testes at different stages of postnatal development: newborn, 20 days after birth and sexually mature rats. in sertoli cells, the distribution of the p125/6.5 antigen parallels [3h]thymidine incorporation: present in newborn and absent in sexually mature testes. the p125/6. ...	1989	2515195
time of insemination and its effect on in-vitro fertilization, cleavage and pregnancy rates in gnrh agonist/hmg-stimulated cycles.	in this prospective study, we compared the effect of delayed inseminations on fertilization, cleavage and pregnancy rates in two groups of patients. in one group, the ovarian stimulation was performed with a clomiphene citrate/human menopausal gonadotrophin/human chorionic gonadotrophin (cc/hmg/hcg) protocol. the other group was pre-treated with gonadotrophin-releasing hormone agonist (gnrha) and ovarian stimulation was carried out with an hmg/hcg protocol. under both protocols, a delay of 2, 4 ...	1989	2515208
the estimation of dietary protein intake in chronic renal failure.	forty-five adult clinic patients with chronic renal failure each supplied a 4-day weighed dietary record, a 24-h urine collection, and a nocturnal spot urine sample. total nitrogen (n) losses derived from the urines were corrected for proteinuria and non-urea nitrogen excretion. individual estimates of n intake were compared by correlation and assessing the level of agreement. daily urea n excretion derived from the spot sample correlated well with the 24-h collection p less than 0.001, but the ...	1989	2515492
the discovery of the enzyme test for rh antibodies.		1989	2515654
[quantitation of human serum apolipoprotein cii by radial immunodiffusion assay].	a radial immunodiffusion assay (rid) for quantitation of human serum apolipoprotein cii (apocii) was developed. antiserum to apocii was raised in goats with purified apocii. the antibody-gel plate was prepared with 1% agarose. 5 microliters of each sample or standard serum was added in a well. diffusion was performed at 25 degrees c or 37 degrees c for 48 h. the standard curve with a working range of 0.8-4.5 mg/dl was plotted. the minimum measurable concentration of apocii was 40 ng. the intra-a ...	1989	2517121
relationship between viral hepatitis and four trace elements in sera.		1989	2517616
survival and growth of food poisoning bacteria following inoculation into cottage cheese varieties.	following inoculation into cottage cheese varieties with and without sorbic acid, obtained directly from the manufacturer, strains of enteropathogenic escherichia coli and other e. coli survived but failed to multiply during storage at 7, 10 or 25 degrees c. in the absence of sorbic acid spoilage due to pseudomonas fluorescens occurred after storage for 5-13 days at 7 or 10 degrees c and 1-2 days at 25 degrees c. salmonella enteritidis, s. hadar, s. saint-paul, s. typhimurium and s. virchow surv ...	1989	2518228
[in vitro susceptibility of campylobacter jejuni and campylobacter coli to quinolone antibiotics].	in this study, in vitro susceptibilities of 24 campylobacter strains isolated from fecal samples were tested for quinolone antibiotics. 13 of the strains were identified as c. jejuni and 11 of them were c. coli. antibiotics which were included in this study were ciprofloxacin, ofloxacin, pefloxacin and fleroxacin. antibiotic susceptibility tests were performed on mueller-hinton agar (supplemented with 5% sheep blood) using the agar dilution method. results showed that quinolone antibiotics are v ...	1989	2518646
furazolidone-induced interstrand cross-links in vibrio cholerae dna. study of conformational change by circular dichroism.	vibrio cholerae dna bearing furazolidone-induced interstand cross-links show a change in the characteristic circular dichroism spectra of the dna itself in dilute buffer. the change in c.d. spectra was characterized by a shift of the positive band around 272 nm to lower wavelength and a loss of ellipticity of the negative band around 242 nm, and is similar to that exhibited by mitomycin linked vibrio cholerae dna under identical conditions and is suggestive of a conformational change of dna bear ...	1989	2518733
[a cytochemical study of lysosomal system in rat junctional epithelium after intravenous horseradish peroxidase injection].	lysosome formation was studied in rat junctional epithelium (je) by detection of acid phosphatase (acpase) using the cerium reaction method after intravenous injection of horseradish peroxidase (hrp). intravenously injected hrp was endocytosed by the whole je, especially at the coronal portion. acpase activity was also observed in numerous organellae (vacuoles, dense bodies, multivesicular bodies and golgi cisternae) in the junctional epithelial cells. these acpase positive organellae often fuse ...	1989	2519301
[acute polyarthritis in a patient with aids. clinical report].	a homosexual patient known to be hiv positive for 2 years developed fever, herpes labialis, salmonellosis and symmetric polyarthritis. no specific cause was documented for the arthritis which was ascribed to the aids syndrome. partial improvement in symptoms was observed after therapy with cotrimoxazole and indomethacin. musculoskeletal complications of aids are reviewed.	1989	2519452
cardiac arrest postoperatively in a patient infected with ascaris, the roundworm.		1989	2519513
removal and reimplantation of the parietal cortex of mice during the first nine days of life: consequences for the barrelfield.	vibrissal follicles on one side of the mouse whiskerpad are topologically connected to barrels in the contralateral somatosensory cortex. barre's develop from postnatal day 3 to 6. recently, i have observed that the barrelfields still develop in pieces of parietal cortex that were removed and reimplanted, in the same place and with the original orientation, on the day of birth, or on postnatal days 1 or 3. now, two questions were asked: (i) can the barrelfield form and/or remain in place after i ...	1989	2519518
killing of human tumor cells by antibody c3b conjugates and human complement.	to potentiate the lytic action of complement on tumor cells, we have constructed heteroconjugates composed of monoclonal antibody and of the human c3b component of complement. the conjugates were formed efficiently using the heterobifunctional cross-linking reagent spdp. the monoclonal antibody-c3b conjugate promoted the killing of k562 tumor cells by normal human serum. treatment of the tumor cells with the monoclonal antibody and normal human serum resulted in 10-15% lysis. however, following ...	1989	2519543
permethrin-impregnated bednets reduce nuisance arthropods in gambian houses.	the prevalence of bedbugs (cimex hemipterus l.), chicken ticks (argas persicus oken) and headlice (pediculus capitis de geer) was surveyed in a rural area of the gambia. at the beginning of the study 37.5% of children's beds were infested with bedbugs and 3.9% with chicken ticks, whilst the prevalence rate of pediculosis in children under 10 years old was 28.8%. both bedbugs and headlice were clustered within compounds. headlice prevalence increased with hair length and they were more common on ...	1989	2519687
neuraminidase pretreatment of donor lymphocytes and graft-versus-host disease.	treatment of murine cba splenocytes with vibrio cholerae neuraminidase (vcn) prior to engraftment into cyclophosphamide (cy) immunosuppressed balb/c x cba mice reduced the incidence of acute lethal graft-versus-host disease (gvhd) and delayed mortality in the later phase of the disease. in the same model, pretreatment of donor splenocytes with vcn also reduced splenomegaly. engraftment of f1 mice with cba cells was clearly demonstrated at day 30 after infusion. treatment of spleen cells with vcn ...	1989	2521829
[non 01 vibrio cholerae septicemia].		1989	2525717
plasmid vectors for constructing translational fusions to the b subunit of cholera toxin.	a family of plasmid cloning vectors has been developed for creating translational fusions to the ctxb gene encoding the b subunit of cholera toxin (ctb) in escherichia coli. these vectors permit insertion of transcriptionally and translationally competent gene sequences upstream from ctxb. to test the utility of the system, a portion of the glucosyltransferase b (gtf) gene (gtfb) from the cariogenic bacterium streptococcus mutans gs-5 (bratthall serotype c), encoding the n-terminal one-third of ...	1989	2531107
muscarinic receptor activation stimulates b-50/gap43 phosphorylation in isolated nerve growth cones.	a characteristic feature of neurite formation is high expression of the phosphoprotein b-50/gap43. previous studies with growth cone membranes have indicated that this neuron-specific protein kinase c substrate may be involved in transmembrane signal transduction at the growth cone. we monitored the degree of phosphorylation of b-50 by quantitative b-50 immunoprecipitation from intact nerve growth cones, isolated from 5-day-old rat brain and prelabeled with 32p-orthophosphate. b-50 phosphorylati ...	1989	2531215
cell surface sialic acid affects immunoglobulin binding to macrophages.	we demonstrate that guinea pig peritoneal macrophages pretreated with neuraminidase from vibrio cholerae bind more 125i-igg than non-treated cells. estimation of binding constants (ka and bmax) shows that the elevation of binding is the result of an increase in affinity and not in the number of receptors for igg. the change of affinity is proportional to amounts of sialic acid liberated from the cells by increasing doses of neuraminidase. it is also shown that affinity of interactions of igg wit ...	1989	2532157
isolation, characterization and immunological determination of basement membrane-associated heparan sulfate proteoglycan.	basement membrane-associated heparan sulfate proteoglycan (hspg) was extracted from isolated porcine glomerular basement membranes and purified by ion-exchange chromatography. the proteogycan was characterized by specific enzymatic digestions, by amino-acid analysis, by sds-polyacrylamide gel electrophoresis and by density gradient centrifugation. polyclonal antibodies were raised against the purified hspg in rabbits. antibodies were characterized by enzyme immunoassays, immunoprecipitation and ...	1989	2533508
ultrastructure of human granulosa cells obtained from follicular fluid aspirates.	samples of the granulosa cells obtained from a punctured human preovulatory follicles stimulated for in vitro fertilization purposes were studied by means of light and electron microscopic methods. early and late preluteinized granulosa cells were characterized by the presence of high amount of pale and homogeneous lipid droplets, moderate accumulation of glycogene and progressive development of smooth endoplasmic reticulum. a new type of granulosa cell containing dense-cored granules with fine ...	1989	2533848
inhibitory effect of dibutyryl cyclic adenosine monophosphate on the induction of alkaline phosphatase in human fetal liver cell line.	when human fetal liver cells (hul-1-317), cultured continuously in a serum-free medium, were incubated with a combination of prednisolone, butyrate and a hypertonic concentration of nacl at 37 degrees c, alkaline phosphatase activity increased. however, the addition of dibutyryl adenosine cyclic monophosphate (bt2camp) to these agents inhibited the increase in alkaline phosphatase activity in a dose-dependent manner: the inhibitory effect of bt2camp was significant at 0.05 mm, but disappeared at ...	1989	2535785
correlation between selective inhibition of the cyclic nucleotide phosphodiesterases and the contractile activity in human pregnant myometrium near term.	the present study was carried out to determine the ability of various pharmacological agents to selectively inhibit each cytosolic form of phosphodiesterase isolated from the longitudinal layer of human myometria near term. among the drugs tested, zaprinast specifically inhibits the first form of pde which hydrolyses both substrates (camp and cgmp) and is stimulated by the ca2+-calmodulin complex. a second form of pde specific for camp hydrolysis and ca2+-calmodulin insensitive is only present d ...	1989	2535936
aggregation by fragilis and non-fragilis bacteroides strains in vitro.	bacteroides fragilis is associated with the formation of intra-abdominal abscesses, whereas other bacteroides species are rarely involved. since bacterial clumping may contribute to the survival of bacteria in the face of host defence mechanisms, the hypothesis has been put forward that differences in aggregation between fragilis and non-fragilis strains of bacteroides may account for their differences in survival in vivo. all seven b. fragilis strains tested formed aggregates within 4 h, but st ...	1989	2536442
bile acid profile in a dog with cholangiocarcinoma.		1989	2536512
crystallization and preliminary x-ray analysis of porin from rhodobacter capsulatus.	porin monomers of the phototrophic bacterium rhodobacter capsulatus were purified. crystals were obtained from a solution of porin solubilized with the detergent octyltetraoxyethylene within 5 days using the vapor phase equilibration technique. the crystals were rhombohedral with an edge length of 0.4 mm. the space group was trigonal r3 with unit cell constants of a = b = 95 a, c = 147 a. reflexions were observed to 3.2 a.	1989	2536626
topographical mapping of calmodulin-target enzyme interaction domains.	calmodulin derivatives, specifically biotinylated in domains i and iii, were synthesized to address the structures of calmodulin necessary for binding to its target enzymes in active conformations. by binding avidin to these biotinylated calmodulins, the role of specific sequences of the calmodulin molecule in target enzyme interactions could then be evaluated. the role of domain i in these interactions was assessed by biotinylation of cys-27 of wheat germ calmodulin with n-ethylmaleimidobiotin. ...	1989	2536706