Publications

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template specificity of the cytoplasmic dna polymerase in xenopus laevis oocytes. 1975167780
molecular weights of estrogen and androgen binding proteins in the liver of xenopus laevis.[3-h]estradiol-17beta and [3-h]dihydrotestosterone binding proteins in the cytosol fraction of liver from both male and female xenopus laevis were characterized by electrophoresis on polyacrylamide gels. these binding proteins, which were indistinguishable based upon their mobilities on gels of different acrylamide concentrations, migrated as single components with a molecular weight of 2.0 x 10-4. separation of native or sodium dodecyl sulfate denatured specific estrogen-binding components on d ...1975167818
[the effects of cortisol and progesterone injected directly into the oocytes of xenopus laevis]. 1975167990
synthesis and secretion of a large glycoprotein in the pars intermedia.the biosynthesis, intracellular transport and release of [3-h]-leucine-containing secretory product has been followed in the pars intermedia of xenopus laevis and, in particular, the synthesis and secretion of a large molecular weight glycoprotein secretory product was demonstrated. however, if xenopus adrenocorticotrophin does contain a leucine residue the results obtained provided no support for the view that it serves as a precursor for melanocytestimulating hormone in this species.1975168285
inhibitor induced alterations of chromatoid bodies in male germ line cells of xenopus laevis.the action of inhibitors of protein synthesis on the structure of cytoplasmic inclusions found in the male germ cell line of the anuran, xenopus laevis, has been studied by light and electron microscopy. results indicate that one such inclusion, the chromatoid body, is sensitive to treatment with either chloramphenicol or puromycin. these drugs administered in vivo or in vitro cause up to a thirty-fold increase in the volume of the chromatoid body in all stages where it is normally present. maxi ...1975169993
mechanism of action of progesterone on amphibian oocytes. uptake and metabolism of progesterone by isolated oocytes of pleurodeles waltlii and xenopus laevis. 1975170033
light-induced formation of dense-core vesicles in rod photoreceptors in retina of xenopus laevis. 1975170537
ultrastructural and pharmacological studies on the afferent synapse of lateral-line sensory cells of the african clawed toad, xenopus laevis.lateral-line sensory cells of xenopus show considerable similarity to central nervous system synapses in their responses to the zinc iodide-osmium tetroxide, and bismuth-iodide cytochemical staining techniques. both reserpine and fla-63 cause a reduction in the diameter and electron density of the synaptic bars, and a dramatic loss of synaptic vesicles surrounding the bars. aminooxy acetic acid and 3-mercaptopropionic acid cause no significant changes in the sensory-cell afferent synaptic appara ...1975171077
changes in the content of cyclic amp and cyclic gmp during the development of xenopus laevis. 1975172338
translation of messenger ribonucleic acid from isolated pancreatic islets and human insulinomas.rna preparations from isolated rat pancreatic islets and from human insulinomas were injected into oocytes of xenopus laevis which were then incubated with [3h]leucine. acid-ethanol extracts of the oocytes were immunoprecipitated with anti-insulin serum using the double antibody technique. sodium dodecyl sulfate disc gel electrophoresis of the immunoprecipitates showed the presence of an insulin-displaceable immunoreactive material with a molecular weight of about 18,000 in extracts from oocytes ...1975174093
[meiosis: role of a histone kinase in the condensation of ovarian oocyte chromosomes of xenopus laevis and ambystoma mexicanum].by injecting heterologous histone-kinase preparations into ovarian axolotl oocytes, it has been possible to speed up the progesterone-induced process of chromosome condensation. moreover, in some instances, this condensation and even complete maturation have been obtained after injection of protein kinase alone, thus in the absence of hormone stimulation. two different histone kinase preparations have been used: one was prepared from ascites cell chromatin and the other from in vitro ovulated xe ...1975177222
[enzymatic aspects of the mitochondria of xenopus laevis oocytes]. 1975183438
[extra- and intra-mitochondrial distribution of respiratory enzymes in oocytes of xenopus laevis]. 1975183439
visual deprivation and intertectal neuronal connexions in xenopus laevis. 19751778
alkaline phosphatase isozymes of xenopus laevis embryos and tissues.alkaline phosphatase was obtained by treating embryos of xenopus laevis with n-butanol at different developmental stages from gastrula to tadpole; the enzyme was also obtained from adult kidney, liver, and intestinal mucosa. purification was carried out by gel filtration and polyacrylamide gel electrophoresis. the enzyme activity is chromatographically spearated into two peaks, with molecular weights of approximately 200,000 and 400,000. alternatively, two groups may be characterized on the basi ...1975237058
the time course of experience-dependent synaptic switching of visual connections in xenopus laevis. 1975237285
osmotic and ionic regulation in xenopus laevis daud. during adaptation to different osmotic environments--v. quantitative alterations of the acid glycosaminoglycans in the kidney. 1975237733
steady state current rectification in potential clamped nodes of ranvier (xenopus laevis).the membrane potential in single nodes of ranvier was changed in rectangular pulse steps while the membrane currents, associated with the potential steps, were measured. changes were made in the ionic composition of the external and the internal medium. the latter changes were obtained by free diffusion through a cut internode. the steady state currents, described on the basis of potassium and leak permeability, were affected by the solution composition in a characteristic way. increased inside ...1975238247
template preferences of dna polymerase and nuclease activities appearing during early development of xenopus laevis. 1975238871
changes in the activity and isozymes of acetyl and butyrylcholinesterase during xenopus laevis development. 1975240618
xenopus laevis cement gland as an experimental model for embryonic differentiation. i. in vitro stimulation of differentiation by ammonium chloride.ectoblastic cells explanted from the animal pole of young xenopus laevis gastrulae have been cultured in vitro. when these cells were cultured for five days in standard salt solutions they formed atypical epidermis. when they were first submitted for 6 h to holtfreter solution containing ammonium chloride and then transferred for five days in standard barth's solution they underwent differentiation into typical cement gland tissue. the optimum concentration of ammonium chloride was 10 mm. below ...1975240903
immunoglobulin heavy chain mrna in mitogen-stimulated b cells.this paper relates the synthesis of dna, immunoglobulin and heavy chain (h) mrna in murine spleen cells following activation of b cells with lipopolysaccharide from e. coli (lps). spleen cells (cba/h mice) were cultivated with 10% fcs and 10 mug lps/ml. 4 h pulses with [3h]thymidine showed that dna synthesis was stimulated within the first day following lps activation and exhibited a sharp peak at 24 h. the shape of the dna synthesis curve suggests that the cells susceptible to lps stimulation a ...1975824129
synthesis of mitochondrial rna by full-grown and maturing oocytes of rana pipiens and xenopus laevis. 19751081057
aspects of tail muscle ultrastructure and its degeneration in rana temporaria.1. tail muscles of larval rana temporaria and to a lesser extent xenopus laevis, at prometamorphosis, were investigated by light and electron microscopy. in the case of rana their degeneration is described at metamorphic climax. 2. the non-degenerate tail muscles of both genera are generally similar in their ultrastructure and likewise similar to those striated muscles of other adult vertebrates including amphibians. 3. at climax tail muscles of rana degenerate by autolysis. it seems likely that ...19751081119
humoral antibodies to soluble antigens in larvae of xenopus laevis. 1975804375
fine structure of the male genital tract and kidney in the anura xenopus laevis daudin, rana temporaria l. and bufo bufo l. under normal and experimental conditions.the ultrastructure of the main cellular components of testicular interstitial spaces in three anuran species was studied during two short periods of the seasonal cycle and after treatment with 5- and 6-hydroxydopamine (5- and 6-ohda). in early december when seminiferous tubules are completely filled with resting spermatozoa in rana, leydig cells display a well developed smooth- and rough-surfaced er, numerous granular vesicles being untypical of mammalian leeydig cells, metochondria with tubular ...1975805666
the macromolecular composition of xenopus laevis egg jelly coat.the three morphologically and functionally distinct jelly coat layers of xenopus laevis eggs, j1, j2, and j3, were separated by manual dissection, solubilized with dithiothreitol, and physicochemically analyzed. the chemical composition of the three jelly layers varied from 37 to 48% protein and 63 to 52% carbohydrate. the carbohydrate consisted of hexosamines, galactose, and fucose. some of the carbohydrate in each of the jelly layers was covalently linked to protein through o-glycosidic bonds ...1975807242
innervation of the male genital tract and kidney in the amphibia, xenopus laevis daudin, rana temporaria l., and bufo bufo l.the innervation of the male genital tract and kidney in three anuran species was studied by the fluorescence histochemical method of falck-hillarp for the demonstration of monoamines whose identity was secured by thin-layer chromatography, and by electron microscopy including administration of 5- or 6- hydroxydopamine (5- and 6-odha). the genital tract comprises testis, intra- and extratesticular and intrarenal seminal efferent ducts, bidder's canal, renal dorsal transverse ducts, and ureter. in ...1975807332
adult amphibian epidermal proteins: biochemical characterization and developmental appearance.the keratin-like proteins (klps) from the epidermis of adult frogs of the species xenopus laevis have been isolated and biochemically characterized by means of polyacrylamide gel electrophoresis, amino acid analysis, tryptic peptide mapping, amino-terminal end-group analysis and isoelectric focusing. one particular protein fraction of rather unusual amino acid composition found only in epidermal tissue was isolated in quantity by preparative gel electrophoresis and monospecific antibodies prepar ...1975810539
biogenesis of plasmalemmal glycoproteins. intracellular site of synthesis of mouse liver plasmalemmal 5'-nucleotidase as determined by the sub-cellular location of messenger rna coding for 5'-nucleotidase.1. free and membrane-bound mouse liver polyribosomes were separated by prolonged density-gradient centrifugation of the post-mitochondrial supernatant. rna was extracted from free and membrane-bound polyribosomes and mrna purified by oligo(dt)-cellulose column chromatography. 2. antisera against purified mouse liver plasma membrane 5'-nucleotidase and moust albumin were prepared and characterized. 3. microinjection of equivalent amounts of mrna from free and membrane-bound liver polyribosomes in ...1975810623
selenium effect on the growth of carcinogenic fungi and cytotoxic action of aflatoxin b1 on lymphocyte culture and on embryonal development of xenopus laevis.the authors report the results of investigations on the protective effect of selenium against teratogenic and cytotoxic action of aflatoxin b1 and the inhibitory action of this element on the growth of fungi producing carcinogenic aflatoxins. sodium selenate inhibited the growth of cultures of such fungi belonging to the class fungi imperfecti as aspergillus flavus, aspergillus fumigatus, penicillium meleagrinum and penicillium rougolosum. added to cell cultures of lymphocytes sodium selenate in ...1975817275
regeneration electrode units: implants for recording from single peripheral nerve fibers in freely moving animals.implantable electrode assemblies that become penetrated by regenerating axons were used to record signals from single sensory and motor nerve fibers associated with leg movement in unrestrained amphibians (xenopus laevis). such neuroimplants may provide a means for establishing the roles of various muscle afferents and efferents in posture and locomotion, and have potential clinical applications.19744809567
double sucrose-gap method applied to single muscle fiber of xenopus laevis.passive electrical properties (internal conductance, membrane conductance, low frequency capacity, and high frequency capacity obtained from the foot of the action potential) of normal and glycerol-treated muscle of xenopus were determined with the intracellular microelectrode technique. the results show that the electrical properties of xenopus muscle are essentially the same as those of frog muscle. characteristics of the action potential of xenopus muscle were also similar to those of frog mu ...19744812637
the effect of catecholamines and background color on the in vitro incorporation of labeled amino acids into the neurointermediate lobe of the pituitary in xenopus laevis. 19744815165
5-methylcytidylic acid: absence from mitochondrial dna of frogs and hela cells.the content of 5-methylcytidylic acid in nuclear dna and mito chondrial dna of xenopus laevis and hela cells has been determined. both nuclear dna's contain 5-methylcytidylic acid. the 5-methylcytidylic acid content of x. laevis dna is 1.7 mole percent of total nucleotides, and that of helc cell dna is 0.7 mole percent. in neither mitochondrial dna could any 5-methylcytidylic acid be detected; the limit of sensitivity was judged at below 0.1 mole percent for x. laevis dna and below 0.05 mole per ...19744815287
incorporation of (3h)uridine and (3h)thymidine during the phase of nucleolar mutliplication in xenopus laevis oögenesis: a high-resolution autoradiographic study. 19744816642
formation and structure of the fertilization envelope in xenopus laevis. 19744822839
the cortical granule reaction in living eggs of the toad, xenopus laevis. 19744822840
an ultrastructural analysis of cell and matrix differentiation during early limb development in xenopus laevis. 19744823502
new membrane formation during cytokinesis in normal and cytochalasin b-treated eggs of xenopus laevis. ii. electrophysiological observations.the electrical membrane potential (e(m)) and electrical membrane resistance (r(m)) were measured continuously during the first cleavage of xenopus eggs, using intracellular microelectrodes. a sharp hyperpolarization of e(m) and decrease in r(m) can be observed from 6 to 8 min after the onset of cleavage. this moment coincides with the onset of the insertion of new membrane (bluemink and de laat, 1973) leading to the formation of the interblastomeric membrane during normal cleavage. removal of th ...19744824285
hatching in the toad xenopus laevis: morphological events and evidence for a hatching enzyme. 19744826290
time of origin of mauthner's neuron in xenopus laevis embryos. 19744831106
gas exchange and its effect on blood gas concentrations in the amphibian, xenopus laevis. 19744832998
an analysis of the degree of homology between 28s rrna from xenopus laevis and xenopus mulleri. 19744834689
polyadenylic acid-containing rna in xenopus laevis oocytes. 19744835729
light microscope autoradiography of ribosomal dna amplification in xenopus laevis. 19744852996
localization of 3h-benzpyrene in the cells of xenopus laevis embryos. 19744853030
nuclear transplantation from specialized and unspecialized gut epithelial cells of adult xenopus laevis. 19744853387
physical properties and protein constituents fo cytoplasmic and mitochondrial ribosomes of xenopus laevis. 19744853633
purification of dna-dependent rna polymerases a and b from ovaries of xenopus laevis. 19744853978
rna metabolism in previtellogenic oocytes of xenopus laevis. 19744854181
the development of the retino-tectal projection in xenopus laevis: an autoradiographic and degeneration study. 19744854715
the effect of nerve growth factor on hindlimb regeneration in xenopus laevis froglets. 19744854766
ultrastructural features of ectodermal--mesenchymal relationships in the developing limb of xenopus laevis. 19744855302
multiple forms of deoxyribonucleic acid-dependent ribonucleic acid polymerase in xenopus laevis. isolation and partial characterization. 19744855626
multiple forms of deoxyribonucleic acid-dependent ribonucleic acid polymerase in xenopus laevis. levels of activity during oocyte and embryonic development. 19744855627
dna-binding proteins of xenopus laevis: synthesis during oogenesis-ovulation and early embryogenesis. 19744855878
a special form of deoxyribonucleic acid dependent ribonucleic acid polymerase from oocytes of xenopus laevis. isolation and characterization. 19744856027
relationship between the amount of the 'germinal plasm' and the number of primordial germ cells in xenopus laevis. 19744856488
on the contents of the cortical granules from xenopus laevis eggs. 19744856980
mitochondrial dna in xenopus laevis oocytes. i. displacement loop occurrence. 19744857493
the translation of mammalian globin mrna injected into fertilized eggs of xenopus laevis. ii. the distribution of globin synthesis in different tissues. 19744857973
resolution of acid-soluble protein component of yolk platelets from xenopus laevis eggs on polyacrylamide gels. 19744857974
ultrastructural changes induced by the fungicide ethylenebis (dithiocarbamic acid)disodium salt (nabm(rabam) in xenopus laevis tissues during development. 19744858828
specificity in deoxyribonucleic acid uptake by transformable haemophilus influenzae.cells of haemophilus influenzae strain rd competent for genetic transformation irreversibly bound approximately five molecular fragments of h. influenzae deoxyribonucleic acid (dna) per cell; under identical conditions, dna derived from escherichia coli b was not taken up (<1 molecule per 50 cells). similarly, dna from xenopus laevis was not taken up by competent h. influenzae. of the heterologous dnas tested, only dna from h. parainfluenzae interfered with the uptake of h. influenzae dna, as ju ...19744597440
detection of haemoglobin in red cells of xenopus laevis by immunofluorescent double labelling. 19744597872
replication and transcription of eukaryotic dna in escherichia coli.fragments of amplified xenopus laevis dna, coding for 18s and 28s ribosomal rna and generated by ecori restriction endonuclease, have been linked in vitro to the bacterial plasmid psc101; and the recombinant molecular species have been introduced into e. coli by transformation. these recombinant plasmids, containing both eukaryotic and prokaryotic dna, replicate stably in e. coli. rna isolated from e. coli minicells harboring the plasmids hybridizes to amplified x. laevis rdna.19744600264
5'-ends of ribosomal and ribosomal precursor rnas form xenopus laevis. 19744600324
the lampbrush chromosomes of xenopus laevis (daudin). 19744609703
the response of xenopus laevis oocytes to injected somatic nuclei soon after germinal vesicle breakdown induced in vitro. 19744609785
ontogeny and localization of the lens crystallins in xenopus laevis lens regeneration. 19744618568
template specificities of xenopus laevis rna polymerases. selective transcription of ribosomal cistrons by rna polymerase a. 19744137235
the preparation of rna-directed dna polymerase from ovaries of xenopus laevis. 19744137289
structural proteins associated with ribosomal cistrons in xenopus laevis chromosomes. 19744138192
analysis of the c-value paradox by molecular hybridization.poly(a)-containing rna was isolated from ovaries of xenopus laevis laevis and triturus cristatus carnifex and used as a template for the synthesis of radioactive complementary dna with rna-dependent dna polymerase. when annealed with an excess of homologous dna, the complementary dna is rendered double-stranded with kinetics that suggest that the coding sequences are single-copy in both these organisms. in triturus, these sequences are distinct from the majority of the genome, which consists of ...19744139720
an ultrastructural study of the subcommissural organ cells of the african clawed toad, xenopus laevis. 19744140039
the location of soluble antigen in the spleen of xenopus laevis. 19744140102
the movement of optically detectable organelles in myelinated axons of xenopus laevis.1. intra-axonal organelles were detected by darkfield and nomarski microscopy in isolated myelinated nerve fibres from xenopus laevis. nerve fibres from the 8th spinal roots, the sciatic nerve, and identified motor and sensory axons from other hind limb nerves were used. the movement of the organelles was recorded either on motion picture film or by noting the times at which they crossed the lines of an ocular grid.2. three groups of organelles were detected in all fibres. a group of particles w ...19744140230
a morphological study of the epithalamus and the thalamus of xenopus laevis. 19744140872
biochemical responses elicited in normal or prolactin-treated xenopus laevis (daudin) specimens by environmental changes. 19744149010
oogenesis in xenopus laevis (daudin). ii. the induction and subcellular localization of tyrosinase activity in developing oocytes. 19744131093
the retinotectal projection from a double-ventral compound eye in xenopus laevis. 19744206638
immunoglobulin classes in xenopus laevis. 19744211233
[energy production during oocyte maturation in xenopus laevis]. 19744211428
the cortical response in xenopus laevis ova. 19744213423
histochemical characteristics of cement-gland mucin in xenopus laevis. 19744275458
mechanical properties of muscle spindles in xenopus laevis. 19744276239
under-replication of ribosomal cistrons in polytene chromosomes of rhynchosciara.dna preparations obtained from several tissues of rhynchosciara americana and two related species, r. milleri and r. papaveroi, were hybridized to r. americana rrna. the percentage of hybridization was found to be higher in tissues with low polyteny than in tissues with high polyteny, suggesting a relationship between the amount of rdna and the tissue polyteny. this could be explained by under-replication of ribosomal cistrons in polytene cells, such as those from the salivary gland. only slight ...19744407049
formation and regression of inappropriate nerve sprouts during trochlear nerve regeneration in xenopus laevis. 19744418001
intercellular gap junctions in pigment epithelium cells during retinal specification in xenopus laevis. 19744423669
the decay of xenopus laevis mitochondrial rrna in the presence of ethidium bromide or actinomycin d. 19744425966
observations on early germ cell development and premeiotic ribosomal dna amplification in xenopus laevis.the origin of premeiotic ribosomal dna (rdna) amplification in germ-line cells of xenopus laevis has been examined using in situ rna-dna hybridization on cytological preparations, tritiated thymidine autoradiography, and isopycnic density gradient centrifugation. primordial germ cells (pgc), from the time they first become localized in the genital ridge at day no. 4 of development, until approximately day no. 22, remain in an extended interphase condition. during this time pgc do not incorporate ...19744426916
ultrastructure of xenopus laevis oocytes after injection of an extract from progesterone-treated oocytes. 19744431074
the effect of hydrocortisone on the sheep red cell response in adult xenopus laevis, the south african clawed toad. 19744436631
an analysis of acidic nuclear proteins during the development of xenopus laevis. 19744442125
hepatic melanosis and ageing in amphibia. a preliminary observation on two species of anura xenopus laevis and rana nigromaculata. 19744442478
metabolism of exogenous 4-14c-progesterone during early development of xenopus laevis. 19744442671
the cyclization of xenopus laevis 5 s dna. 19744444032
secondary structure maps of ribosomal rna and dna. i. processing of xenopus laevis ribosomal rna and structure of single-stranded ribosomal dna. 19744444053
[deranged axial growth of the xenopus laevis larva following elimination of the subcommissural organ]. 19744445814
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