Publications

[chemical and serological studies of lipopolysaccharides isolated from o1 and non-o1 vibrio cholerae].		1990	2082025
[relationship between the anti-hemolysin activity and the structure of catechins and theaflavins].	we examined the corresponding isomers of catechins and theaflavins for anti-hemolysin activities against staphylococcus aureus alpha-toxin and vibrio cholerae o1 hemolysin. catechins and theaflavins showed anti-hemolysin activities in a dose-dependent manner. among the catechins tested, (-)catechin gallate, (-)epicatechin gallate and (-)epigallocatechin gallate having galloyl groups in their molecules showed more potent anti-hemolysin activities against both toxins. on the other hand, free catec ...	1990	2082027
detection and interpolation of outliers in biosignals.	detection of outliers in recorded biosignals and their interpolation is described. the detection of outliers is based on the simple rule of 3 sigma. the subsequent interpolation is performed according to the method proposed by brubacher and wilson for autoregressive processes. the output of the suggested procedure can be treated by standard programs of statistical analysis as long as the influence of outliers is eliminated. the program in fortran iv developed in the institute of hygiene and epid ...	1990	2082646
effect of alprazolam on pentylenetetrazol convulsions.		1990	2082648
randomized double-blind placebo controlled trial to evaluate the safety and immunogenicity of the live oral cholera vaccine strain cvd 103-hgr in swiss adults.	a randomized, double-blind, placebo controlled trial was conducted in 50 healthy swiss adults to assess the safety and immunogenicity of the live oral attenuated cholera vaccine candidate strain vibrio cholerae cvd 103-hgr (classical, inaba). a single dose of 5 x 10(8) viable cvd 103-hgr organisms, administered in a buffered liquid formulation, was well tolerated as compared with individuals who received an equivalent amount of heat-killed escherichia coli k-12 placebo. eighty-eight percent of s ...	1990	2087879
immunogenicity of liposome-associated oral cholera vaccine prepared from combined vibrio cholerae antigens.	liposomes were prepared from bovine brain sphingomyelin and cholesterol. they were reinforced by incorporation of osmium tetroxide to prevent their immediate degradation inside the host. combined vibrio cholerae antigens (lipopolysaccharide, crude cell-bound hemagglutinin and procholeragenoid) were orally administered to experimental rats either as free or liposome-associated. a total of 70 experimental rats was utilized in experiments comparing the immune responses of rats to liposome-associate ...	1990	2091663
the morphology of synovial lining of various structures in several species as observed with scanning electron microscopy.	data concerning surface morphology of synovial lining of tendons, tendon sheaths, cruciate ligaments, infra-patellar fat pads and peripatellar synovial ridges in various species (rat, rabbit, dwarf goat, sheep, pig, dog, human) are reported on. supportive studies with transmission electron microscopy (tem) and light microscopy were performed. three principal morphological appearances of the synovium are evident. on structures with a dense fibrous architecture like tendons, tendon sheaths and cru ...	1990	2094014
the mechanism of inhibition of s-adenosyl-l-homocysteine hydrolase by fluorine-containing adenosine analogs.	(z)-4',5'-didehydro-5'-deoxy-5'-fluoroadenosine (i), 5'-deoxy-5'-difluoroadenosine (ii), and 4',5'-didehydro-5'-deoxy-5'-fluoroarabinosyl-adenosine (iii) are inhibitors of rat liver s-adenosyl-l-homocysteine hydrolase. compounds i and ii are time-dependent and irreversible inhibitors of the enzyme. both i and ii are oxidized by e.nad to produce e.nadh, and fluoride anion is formed in the inactivation reaction (0.7 to 1.0 mole fluoride/mole of enzyme subunit, and 1.7 moles fluoride/mole of enzyme ...	1990	2094766
[aminoacyl-trna synthetases (codases) and their noncanonical functions].	the aim of this review is to summarize the data obtained in the author's laboratory during the last decade. the main objects of these investigations were mammalian aminoacyl-trna synthetases, mainly bovine tryptophanyl-trna synthetase (ec 6.1.1.2). the data are discussed and compared with those described in literature. in the course of these studies it turned out that some properties of mammalian aminoacyl-trna synthetases for instance, nuclear location of some of the synthetases, presence of ex ...	1990	2094804
spinal cord repair: is tissue oxygenation an important variable?	we have demonstrated the reliability and feasibility of making pto2 recordings from graft and host tissue in the injured spinal cord. the data suggest that the oxygen microenvironment of developing graft and host spinal tissue is clearly different from that found in normal spinal tissue or in transplants that have not survived or integrated well. these same constraints seem to apply to cavitation in developing grafts and poorly developed graft/host interfaces. the similarity between these findin ...	1990	2096642
epidemiological profile of outbreaks of cholera in india during 1975-1989.	cholera has been present in india since antiquity. six pandemics originated in indian subcontinent. the present seventh pandemic caused by el tor vibrio cholerae started from indonesia (sulawesi) in 1961 and entered india in 1964. by the end of 1965 it has replaced the age old classical v. cholerae. many of the states which never had cholera or were free from it for a long time got infected and became endemic foci of el tor infection. this article reviews the epidemiological features of importan ...	1990	2098418
the occurrence of alpha (1----2) linked n-acetylperosamine--homopolymer in lipopolysaccharides of non-o1 vibrio cholerae possessing an antigenic factor in common with o1 v. cholerae.	chemical analysis was carried out on lipopolysaccharides from vibrio cholerae bio-serogroup hakata 487-85. the o-specific chain of the phenol-soluble lipopolysaccharides was demonstrated by 13c-nmr spectroscopy and methylation analysis to contain a linear homopolymer of alpha(1----2) linked n-acetylperosamine (4-acetamido-4,6-dideoxy-d-mannopyranose), which was closely similar to but not identical to a linear alpha(1----2) linked n-3-deoxy-l-glycerotetronyl (s-2,4-dihydroxybutyryl) perosamine-ho ...	1990	2098633
[the determination of the optimal inoculation dose of an oral cholera chemical bivalent vaccine in a controlled experiment].	276 volunteers aged 19 years and over were placed under observation in the course of the trial of oral cholera vaccine in tablets, containing choleragen toxoid, o-antigens of serovars inaba and ogawa and a number of vibrio cholerae exoenzymes, for safety, reactogenic properties and immunological effectiveness. the vaccine was found to produce no reactions in a dose of 1-4 tablets; the administration of 3 tablets (300,000 binding units of the toxoid and 10,000 units of o-antigens, serovars inaba ...	1990	2099071
[the effect of immune antibodies and the xanthine oxidase-xanthine enzymatic link on vibrio cholerae].	as revealed in experiments on v. cholerae, highly diluted cholera antiserum enhanced the inhibitory action of the enzymatic link xanthine oxidase-xanthine-fe2+ on the multiplication of v. cholerae, while low dilutions of the antiserum weakened this action. normal rabbit serum produced no such effect. the antivibrionic effectiveness of the immune molecular cycle, viz. antiserum--the xanthine oxidase enzymatic link, was found to depend also on the concentration of xanthine. immune antibodies to ch ...	1990	2099072
characterization of vibrio cholerae non-o1 hemagglutinin/protease.		1990	2101140
cloning and nucleotide sequence determination of a heat-stable enterotoxin gene from vibrio cholerae non-o1.		1990	2101148
management of acute diarrhoea.	before 1970, laboratory staff could not only identify the causative organism of acute diarrhea in 20% of cases, but in 1990, they could identify it in 80% of cases. these organisms are either bacteria, virus, or parasites. the bacteria include enterotoxigenic bacteria (vibrio cholerae, enterotoxigenic escherichia coli, clostridium perfringens, and staphylococcus aureus) and enteroinvasive bacteria (campylobacter jejuni, c. coli, and salmonella and shigella species). the leading cause of death ...	1990	2101385
etiological agents of diarrhoea.	two decades of research have established newer pathogens and techniques in establishing several organisms of diarrhoeal diseases as aetiological agents. it is now possible to detect an agent in 80% of the situation of diarrhoea in a standard laboratory. the brief review describes the list of pathogens, their diagnostic techniques with short description on clinical and epidemiological status.	1990	2101388
effectiveness of oral rehydration salt solution (ors) in reduction of death during cholera epidemic.	an extensive outbreak of acute gastroenteritis involving all age group of patients occurred during july-september, 1978 in the central district of manipur state. a total of 4469 cases occurred during the period. 45.7% of diarrhoea cases sampled and 47.6% of water samples collected from rivers were found to be positive for v.cholerae biotype eitor. case fatality rate in this epidemic was exceptionally low (0.8%) which was attributed to the early domiciliary use of oral rehydration salt solution ( ...	1990	2101392
construction of an invertible dna segment for improved antigen expression by a hybrid salmonella vaccine strain.		1990	2101464
new attenuated derivatives of vibrio cholerae.		1990	2101479
recombinant attenuated vibrio cholerae strains used as live oral vaccines.	although great strides have been made in the development of recombinant attenuated vibrio cholerae vaccine strains, the task has not been as simple as once imagined. the initial vaccine candidates proved to be unexpectedly reactogenic but further derivatives, such as cvd103-hgr, are well-tolerated, immunogenic and protective after a single dose. in addition, this strain carries a selectable marker to distinguish it from wild strains and has been evaluated in a practical, lyophilized formulation ...	1990	2101480
delivery of the cholera toxin b subunit by using a recombinant yersinia enterocolitica strain as a live oral carrier.	the gene ctxb encoding the cholera toxin b subunit was subcloned to design its production by yersinia enterocolitica. it was joined in two ways to yoph, a gene of the virulence plasmid pyv specific to this genus. this gene encodes one of the major yop proteins (yoph) secreted by bacteria incubated at 37 degrees c in a ca(2+)-deprived medium. in a first construction, an operon fusion was obtained between ctxb and yoph so that ct-b and a truncated yoph protein were produced. the recombinant ct-b f ...	1990	2101483
a study of v. cholerae strains isolated in bombay.	of 935 faecal samples studied over a period of one year, v. cholerae 01 was isolated from 102 samples (10.9%). all the strains were found to be e1 tor ogawa. the strains belonging to the phage types 2 and 4 were encountered in our study, type 2 being the highest (76.5%). the sensitivity pattern of all strains to the commonly used antibiotics was determined. strains sensitive to gentamicin (92.2%), nalidixic acid (85.3%), kanamycin (83.3%), cotrimoxazole (80.4%) and chloramphenicol (75.5%) were o ...	1990	2102910
neuroblastoma differentiation involves the expression of two isoforms of the alpha-subunit of go.	the regulation of gtp-binding proteins (g proteins) was examined during the course of differentiation of neuroblastoma n1e-115 cells. n1e-115 cell membranes possess three bordetella pertussis toxin (ptx) substrates assigned to alpha-subunits (g alpha) of go (a g protein of unknown function) and "gi (a g protein inhibitory to adenylate cyclase)-like" proteins and one substrate of vibrio cholerae toxin corresponding to an alpha-subunit of gs (a g protein stimulatory to adenylate cyclase). in undif ...	1990	2107277
long-term persistence of toxigenic vibrio cholerae 01 in the mucilaginous sheath of a blue-green alga, anabaena variabilis.	cholera epidemics occur twice a year in the endemic area of bangladesh. vibrio cholerae 01 can be isolated from the environment only during the epidemics and the question of possible interepidemic environmental reservoirs of v. cholerae remains open. the present laboratory-based studies investigate the role of an aquatic alga, anabaena variabilis, as a possible reservoir. persistence of v. cholerae inside the mucilaginous sheath of a. variabilis was observed by phase-contrast and fluorescent mic ...	1990	2109096
closely related glycosylation patterns of recombinant human il-2 expressed in a cho cell line and natural il-2.	we report here the study of the glycosylation pattern of human recombinant (r) il2 expressed in a chinese hamster ovary (cho) cell line. the human ril2 secreted by this high-producing recombinant cho cell line was metabolically radiolabelled with [35s]-methionine, or with [3h]-glucosamine and [3h]-galactose, purified to homogeneity, and then characterized. the electrophoretic analysis of the [35s]-methionine-labelled proteins present in the culture medium of the cho cell line showed that the ril ...	1990	2109157
a single pre-b cell can give rise to antigen-specific b cells that utilize distinct immunoglobulin gene rearrangements.	a group of hybridomas that express antibodies with related specificities for the influenza virus hemagglutinin (ha), that represent b cells that were the clonal progeny of a single pre-b cell, and that utilized distinct l chain gene rearrangements have been characterized. the clonal relationship was established by the sharing of h chain gene rearrangements at both the productive and the nonproductive alleles. among these hybridomas, one group had rearranged only one of its kappa alleles, having ...	1990	2117635
a single pre-b cell can give rise to antigen-specific b cells that utilize distinct immunoglobulin gene rearrangements.	a group of hybridomas that express antibodies with related specificities for the influenza virus hemagglutinin (ha), that represent b cells that were the clonal progeny of a single pre-b cell, and that utilized distinct l chain gene rearrangements have been characterized. the clonal relationship was established by the sharing of h chain gene rearrangements at both the productive and the nonproductive alleles. among these hybridomas, one group had rearranged only one of its kappa alleles, having ...	1990	2117635
properties of a hemolysin related to the thermostable direct hemolysin produced by a kanagawa phenomenon negative, clinical isolate of vibrio parahaemolyticus.	a hemolytic toxin (vp-trh) produced by a kanagawa phenomenon negative, clinical isolate of vibrio parahaemolyticus was further characterized. the purified vp-trh showed various biological activities, such as fluid accumulation in rabbit ileal loops, increase of rabbit skin vascular permeability, and cardiotoxicity on cultured myocardial cells, all of which are essentially similar to the activities found with thermostable direct hemolysin (vp-tdh), a pathogenic toxin produced by kanagawa phenomen ...	1990	2118824
polarized secretion of diamine oxidase by intestinal epithelial cells and its stimulation by heparin.	the caco-2 cells have been used as a model system to study the pathways of diamine oxidase secretion by the intestinal epithelium. when grown in transwell filter chamber devices, the polarized cell monolayers released the enzyme preferentially into the basal chamber. heparin (1-10 usp u/ml) rapidly induced a marked stimulation of enzyme secretion only when in contact with the basolateral cell membrane, where high affinity binding sites for [3h]heparin were also exclusively located. among the oth ...	1990	2121582
comparison of the vibrio cholerae hemagglutinin/protease and the pseudomonas aeruginosa elastase.	the soluble hemagglutinin/protease (ha/protease) produced by vibrio cholerae and the elastase of pseudomonas aeruginosa are both zinc/calcium-dependent proteases. in the present study the two enzymes are compared immunologically and functionally. the n-terminal amino acid sequences of the proteins had 65% identity within the first 20 amino acids. polyclonal antisera against each purified protein recognized the enzyme of the other species in enzyme-linked immunosorbent assay, checkerboard immunob ...	1990	2123831
[purification and various properties of vibrio cholerae non-01 cytolysin].	a new method for isolation and purification of vibrio cholerae non-01 cytolysin has been elaborated. it includes the steps of concentration by ammonium sulphate, ion exchange chromatography on de-52-cellulose, gel filtration via ultragel asa-44 and chromatography on mono q. cytolysin is shown to be a 60 kd and pi 6.2 protein. it is not inactivated by thiol-restoring agents and induces the production of precipitating antibodies. in contrast to choleragen the protein cytolytically affects erythroc ...	1990	2123963
expression of toxr, the transcriptional activator of the virulence factors in vibrio cholerae, is modulated by the heat shock response.	the toxr gene of vibrio cholerae encodes a transmembrane, dna-binding protein that positively controls transcription of the genes for cholera toxin, tcp pili, and other proteins important in cholera pathogenesis. nucleotide sequence analysis of the toxr upstream region has revealed that the heat shock gene htpg, encoding the bacterial homologue of the eukaryotic hsp90 protein, was located immediately upstream and was divergently transcribed from toxr. using lacz transcriptional fusions, we have ...	1990	2124707
telomere and centromere dna are associated with the cores of meiotic prophase chromosomes.	mouse (mus musculus) whole-mount, surface-spread, meiotic prophase chromosomes have an axial core structure, the synaptonemal complex, sc, from which extend chromatin loops. this arrangement permits a novel approach to the analysis of chromosome structure. using in situ hybridization, the types of dna sequences preferentially associated with the sc and the types located primarily in the chromatin loops can be determined. with biotinylated probes, detected by avidin conjugated to fitc, we present ...	1990	2129288
[presence of salmonella in the northwest shore of the l'albufera de valencia].	for the purpose of ascertaining the amount of pollution reaching the large salt lagoon in valencia from its northwest side, 19 river-beds flowing into it have been studied. for each sample, the presence of markers indicating fecal pollution and the presence or absence of pathogens; salmonella, vibrio cholerae and shigella have been evaluated. for each river-bed, a fecal pollution index has been calculated, this index revealing a positive correlation (p = 0.03) with the isolating of salmonella. s ...	1990	2131632
1,25 dihydroxycholecalciferol and the genesis of hypocalcaemia in magnesium-deficient chicks.	the role of 1,25 dihydroxycholecalciferol (1,25 dhcc) in the genesis of hypocalcaemia of magnesium (mg) deficiency was studied in chicks fed control or mg-deficient diets with varying calcium (ca) content. in one study, ca and mg deficiencies were induced simultaneously whereas in a second study, exposure to low dietary ca followed induction of mg deficiency. in both studies, mg-deficient chicks fed adequate dietary ca were hypocalcaemic yet had normal circulating 1,25 dhcc, whereas mg-deficient ...	1990	2132748
kinetic analysis of 3-quinuclidinyl 4-[125i]iodobenzilate transport and specific binding to muscarinic acetylcholine receptor in rat brain in vivo: implications for human studies.	radioiodinated r- and s-quinuclidinyl derivatives of rs-benzilate (r- and s-125iqnb) have been synthesized for quantitative evaluation of muscarinic acetylcholine receptor binding in vivo. two sets of experiments were performed in rats. the first involved determining the metabolite-corrected blood concentration and tissue distribution of tracer r-iqnb (active enantiomer) and s-iqnb (inactive enantiomer) in brain 1 min to 26 h after intravenous injection. the second involved the measurement of br ...	1990	2134838
surfaces of murine lymphocyte subsets differ in sialylation states and antigen distribution of a major n-linked penultimate saccharide structure.	rat liver beta-galactoside alpha-2,6-sialyltransferase and vibrio cholerae sialidase were used with cytidine-5'-monophospho-n-acetyl-[3h]neuraminic acid (cmp-[3h]neuac) to specifically probe the distribution and sialylation state of gal beta 1-4glcnac residues on n-linked saccharides on the surfaces of murine lymphocytes. the relative extent of exogenous sialyltransferase-mediated sialylation (per cellular protein) was thymocytes greater than t-cells greater than t-cell lymphoma (el-4) greater t ...	1990	2137033
ca2(+)-dependent membrane-binding proteins in normal erythrocytes and erythrocytes from patients with chronic myelogenous leukemia.	cytosolic and membrane fractions of human erythrocytes were probed with antisera raised against several members of the annexin family of ca2(+)-dependent phospholipid/membrane-binding proteins. one of the antisera, that against the 67 kd calcimedin, identified erythrocyte polypeptides of molecular weights 48 and 67 kd, which were found in the cytoplasm when normal erythrocytes were lysed in the presence of egta but on the membrane when lysis buffers contained ca2+. in contrast, membranes of eryt ...	1990	2138037
serious adverse drug reactions to pyrimethamine-sulphadoxine, pyrimethamine-dapsone and to amodiaquine in britain.	all reports of adverse reactions with pyrimethamine-sulphadoxine (fansidar), pyrimethamine-dapsone (maloprim), and amodiaquine spontaneously reported through the uk national post-marketing system were reviewed. retrospective reporting rates of serious reactions associated with these drugs were analysed using prescription data from the department of health, derived from the prescription pricing authority, and relevant pharmaceutical companies. whilst interpretation of these data requires caution, ...	1990	2138674
the glycosyl phosphatidylinositol-linked fc gamma riiipmn mediates transmembrane signaling events distinct from fc gamma rii.	to investigate the ability of fcgammariii(pmn), the gpi-anchored isoform of fcgammariii (cd16) in polymorphonuclear leukocytes (pmn), to mediate transmembrane signaling events, we measured changes in membrane potential with dioc(5) and in intracellular calcium with indo-1. fcgammar were ligated by anti-fcgammariii mab 3g8 (igg and fab), anti-fcgammarii mab iv.3 (igg and fab), and human igg aggregates. cell bound mabs were also crosslinked by goat f(ab')(2) anti-mouse igg. 3g8 igg elicited a rapi ...	1990	2139101
activation of the e2f transcription factor in adenovirus-infected cells involves e1a-dependent stimulation of dna-binding activity and induction of cooperative binding mediated by an e4 gene product.	previous experiments have demonstrated that the dna-binding activity of the e2f transcription factor is increased upon adenovirus infection and that both the e1a and e4 genes are required for activation. in this study, we demonstrated that this enhanced binding of e2f to the e2 promoter is the result of two events. (i) there is stimulation of the dna-binding activity of the e2f factor; this stimulation is e1a dependent but independent of e4. (ii) there is also induction of a stabilized interacti ...	1990	2139893
dna damage and prophage induction and toxicity of nitrofurantoin in escherichia coli and vibrio cholerae cells.	repair-deficient and repair-proficient strains of e. coli k12 were sensitive to nitrofurantoin treatment to varying degrees with the double mutant strain (uvra 6, reca 13) being most sensitive. ultraviolet absorption data and thermal chromatography through a hydroxyapatite column revealed that nitrofurantoin treatment of v. cholerae strain ogawa 154 produced a maximal amount of 55% reversibly bihelical dna at a nitrofurantoin dose of 120 micrograms/ml/h, which indicated the formation of inter-st ...	1990	2139919
contribution of u1 snrna structural domains to u1 snrnp function.		1990	2141898
influence of chronic alterations of salt intake and aging on the kinetic of red cell na+ and k+ transport in sprague-dawley rats.	the kinetics of na+ and k+ (rb)+ transport mediated by the na(+)-k+ pump and na(+)-k+ cotransport system (assessed as a function of rb+o and na+i) as well as the magnitude of cation leaks were determined in red cells of young male rats subjected to chronic salt deprivation or salt loading (0.1% and 8% nacl diet). these salt intake alterations induced moderate kinetic changes of the na(+)-k+ pump which did not result in significant changes of ouabain-sensitive (os) rb+ uptake or na+ net extrusion ...	1990	2142786
stimulation of a human erythrocyte membrane atpase by glutathione conjugates.	an atp-dependent transport process for s-(2,4-dinitrophenyl) glutathione (dnp-sg) mediated by a novel atpase designated as dnp-sg atpase has been demonstrated in human erythrocytes (labelle et al., febs lett. 228, 53-51, 1988). in order to investigate whether the dnp-sg atpase system represents a generalized mechanism for the transport of xenobiotic conjugates of glutathione (gsh), stimulation of this atpase by different gsh conjugates was studied in membrane vesicles prepared from human erythro ...	1990	2143605
[comparative study of cholera phages isolated from the water of open reservoirs and lysogenic strains of vibrio el-tor].		1990	2144501
iron-binding compounds and related outer membrane proteins in vibrio cholerae non-o1 strains from aquatic environments.	a total of 156 strains of vibrio cholerae non-o1 from aquatic origins were examined for the presence of iron uptake mechanisms and compared with o1 strains and other vibrio species. all non-o1 strains were able to grow in iron-limiting conditions, with mics of ethylenediaminedi (o-hydroxyphenylacetic acid) ranging from 20 microm to 2 mm. the production of siderophores was demonstrated by growth in chrome azurol s agar and cross-feeding assays. all strains produced phenolate-type compounds, as as ...	1990	2144956
[the epidemiological importance of vibrio cholerae isolated from different ecological systems].	the analysis of the data on the isolation of v. cholerae from different ecological systems indicates that v. eltor do not constantly inhibit the rivers and sea at the territory under control. hemolytically active v. cholerae without the vct gene, found to be faintly virulent and avirulent when studied on suckling rabbits used as a model and when evaluated by the complex method, show no tendency towards epidemic spread in the presence of conditions for the realization of the transmission of vibri ...	1990	2146847
preparation and characterization of anti-paroxetine antibodies.	6-nitroparoxetine was synthesized and reduced to 6-aminoparoxetine. after coupling to glutaraldehyde at the 6-position and to bovine serum albumin, the resulting schiff's base was further reduced into an amino-derivative which served as the antigen. anti-paroxetine antibodies were raised against this antigen in rabbits and the anti-paroxetine igg purified by protein a affinity chromatography. the anti-paroxetine igg demonstrated high specificity towards paroxetine and 6-nitroparoxetine without s ...	1990	2147049
[the epidemiological aspects of the importation of cholera into the territory of the uzbek ssr in recent years].		1990	2150142
[sensitivity of enteropathogenic bacteria to furazolidone].	the sensitivity in vitro of 348 strains of 18 enteropathogen agents to furazolidone was investigated during the period of 1987-1989. all strains of shigella sonnei (17), s. flexneri (17), s. boydii (16), escherichia coli enteropathogen (40), e. coli enteroinvasive (20), campylobacter jejuni (50), vibrio cholerae 01 (5), vibrio cholerae non 01 (5), v. parahaemolyticus (5), v. alginolyticus (2), aeromonas hydrophila (5), a. caviae (5), a. sobria (5), and plesiomonas shigelloides (12) were sensitiv ...	1990	2152485
identification of an iron-regulated virulence determinant in vibrio cholerae, using tnphoa mutagenesis.	several virulence determinants of bacteria are regulated by the concentration of iron in the medium, with increased expression occurring under low-iron conditions. iron-regulated virulence factors have not been previously described in vibrio cholerae. we used the transposon vector tn5 is50l::phoa (tnphoa) to obtain insertion mutations in iron-regulated genes of v. cholerae 0395. one strain, carrying an insertion mutation in iron-regulated gene irga, had reduced virulence in an animal model and h ...	1990	2152889
interaction of cholera toxin with cloned human goblet cells in monolayer culture.	exposure of the intestinal mucosa to vibrio cholerae enterotoxin (ct) results in mucus secretion from intestinal goblet cells. on the other hand, there is evidence that elevation of intracellular adenosine 3',5'-cyclic monophosphate levels is not sufficient to induce rapid mucin secretion. to determine whether ct has direct effects on human goblet cells and whether ct alone can elicit rapid exocytosis of apical mucin granules, purified ct was applied to monolayer cultures of well-differentiated ...	1990	2154122
comparative studies of azithromycin in skin and soft-tissue infections and sexually transmitted infections by neisseria and chlamydia species.	two open, randomized, single centre studies have investigated the efficacy and safety of azithromycin (cp-62,993) in the treatment of infections by azithromycin-sensitive pathogens: (a) acute bacterial infections of skin or soft tissue (compared with erythromycin; n = 82); and (b) urethritis and/or cervicitis caused by neisseria gonorrhoeae and/or chlamydia trachomatis (compared with doxycycline; n = 108). in study a, azithromycin was administered to 42 patients for five days at a dosage of 250 ...	1990	2154430
future vaccines against enteric pathogens.	a small number of bacterial agents, including enterotoxigenic escherichia coli (etec), enteropathogenic e. coli (epec), shigella and vibrio cholerae 01 and one virus, rotavirus, combine to cause a major proportion of the diarrheal illness of public health importance worldwide. salmonella typhi is by far the major cause of enteric fever. attempts to develop safe, practical, and effective vaccines against these agents are under way. examples of vaccine candidates include live oral vaccines against ...	1990	2155260
characterization of the hlyb gene and its role in the production of the el tor haemolysin of vibrio cholerae o1.	el tor strains of vibrio cholerae are capable of producing a haemolysin which they actively secrete into the growth medium. this requires translation to produce the protein at the surface of the cytoplasmic membrane and translocation across this membrane, the periplasmic space and the outer membrane. the mechanism by which this occurs is poorly understood. in addition to the structural gene for the haemolysin (hlya), we have cloned a second adjacent gene, hlyb. by site-directed mutagenesis, spec ...	1990	2162464
induction of ornithine decarboxylase mrna in cerebral cortex in response to lesion of nucleus basalis.		1990	2164982
subunit analysis of bovine cytochrome c oxidase by reverse phase high performance liquid chromatography.	bovine cytochrome c oxidase subunits were separated by reverse phase high performance liquid chromatography using a c4 column eluted with water and an acetonitrile gradient, both containing 0.1% trifluoroacetic acid. subunits i and iii precipitated in this solvent and could not be analyzed; the remaining eleven subunits were dissociated, denatured, soluble and could be resolved by elution from the column. the protein subunit eluting in each chromatographic peak was identified by a combination of ...	1990	2168156
kinetics of the reaction of prostaglandin h synthase compound ii with ascorbic acid.	the reduction of prostaglandin h synthase compound ii by ascorbic acid in the presence of diethyldithiocarbamate was studied in 0.1 m phosphate buffer (ph 8.0) at 4.0 +/- 0.5 degrees c, by rapid scan spectrometry and transient state kinetics. a saturation effect and nonzero intercept were observed in the plot of pseudo-first-order rate constant versus ascorbic acid concentration. the saturation behavior suggests formation of a complex between prostaglandin h synthase compound ii and ascorbic aci ...	1990	2168160
presence of human papillomavirus type-6-related sequences in inverted nasal papillomas.	twenty dna samples obtained from seven cases of inverted papillomas, eight cases of nasal polyps and five cases of chronic sinusitis were investigated by southern blot hybridization for the possible presence of sequences homologous to human papillomavirus (hpv) types 6, 11, 16 and 18. hpv type-6-related dna was identified in one of the seven inverted papillomas. the restriction endonuclease cleavage patterns showed that this latter dna is a new subtype of hpv type 6 dna. in the other six papillo ...	1990	2168185
vectorial electron and proton transfer steps in the cytochrome bc1 complex.		1990	2168207
a progesterone-modulated, low-molecular-weight protein from the uterus of the sheep is associated with crystalline inclusion bodies in uterine epithelium and embryonic trophectoderm.	a protein of mr 14,000 (14k protein) has been identified in the medium after culturing sheep conceptuses in vitro. however, it was also a component of uterine flushes of nonpregnant ewes during the luteal phase of the estrous cycle. the 14k protein, which consists of several isoforms, was purified from conceptus culture medium by a simple three-step procedure, involving ammonium sulfate precipitation, high-performance anion-exchange chromatography, and gel filtration on sephacryl s-200. western ...	1990	2168219
cytomegalovirus nucleic acids in allografted hearts.		1990	2168339
enhancement by calcium of the detection of cytomegalovirus in cells treated with dexamethasone and dimethyl sulfoxide.	mrc-5 cultures treated with dexamethasone, dimethyl sulfoxide, and calcium were compared with untreated cultures and cultures treated with dexamethasone and dimethyl sulfoxide for the detection of human cytomegalovirus by the shell vial method. the addition of calcium resulted in significantly increased growth of human cytomegalovirus ad169. protein studies and cell counts showed renewed cell proliferation in the treated cultures. when low-titer clinical specimens were retested, 27.5% of all pos ...	1990	2168437
accuracy and precision of computer-simulated tissue temperatures in individual human intracranial tumours treated with interstitial hyperthermia.	accurate knowledge of tissue temperature is necessary for effective delivery of clinical hyperthermia in the treatment of malignant tumours. this report compares computer-predicted versus measured intratumoral temperatures in 11 human subjects with intracranial tumours, treated with a conceptually simple 'conductive' interstitial hyperthermia system. interstitial hyperthermia was achieved by the use of parallel arrays of implanted, electrically heated catheters. the tissue was warmed by thermal ...	1990	2168460
urinary bladder blood flow. i. comparison of clearance of locally injected 99mtechnetium pertechnate and radioactive microsphere technique in dogs.	the blood flow of the dog urinary bladder measured by radioactive microsphere technique was compared to the clearance of locally injected 99mtechnetium pertechnate (99mtc) in the bladder wall. in semilogarithmic plots the 99mtc washout curves showed a multiexponential course. from the initial slopes (median 5.7 min) the bladder blood flow was calculated to be only 30-62% of the results obtained from the radioactive microsphere technique (blood flow in the muscular layer 21.7-44.8 ml/100 g/min). ...	1990	2168600
protective effect of inactivated pasteurella haemolytica bacterin challenged in bovine herpesvirus-1 experimentally infected calves.	formalin-inactivated and sonicated pasteurella haemolytica bacterins were prepared from 1 h cultures of bacterial pellets in rpmi-1640 medium containing 7% fetal calf serum. the bacterial pellets were obtained from logarithmic phase growth of the organism by centrifugation. the protective effect of the vaccine was evaluated in 43 specific-pathogen-free hereford crossbred calves and yearlings in three experiments. cattle were either single vaccinated or boosted via three routes; intratracheally ( ...	1990	2168606
increase of the anion and proton permeability of saccharomyces carlsbergensis plasmalemma by n-alcohols as a possible cause of its de-energization.	the effect of n-alcohols on atp-dependent generation of delta ph and em across the plasma membrane vesicles of the yeast saccharomyces carlsbergensis was investigated. the alcohols were shown to collapse delta ph and em in the order c2 less than c3 less than c4 less than c5 less than or equal to c6 greater than or equal to c7 greater than c8 greater than c11, the dissipation of em being more pronounced. inhibition of the plasmalemma h(+)-atpase was insignificant; at low alcohol concentrations it ...	1990	2168610
mechanisms underlying the stimulation of pth release by gppnhp in permeabilized bovine parathyroid cells.	we examined changes in camp and inositol phosphate metabolism to assess the contribution of the guanine nucleotide regulatory (g) protein(s) regulating adenylate cyclase and phospholipase c in mediating the stimulatory effects of gppnhp on pth release from permeabilized bovine parathyroid cells. to examine the role of gs, the g protein stimulating adenylate cyclase, and camp on pth release, permeabilized cells were incubated with either gppnhp or isoproterenol, and the effects of these agents on ...	1990	2168660
effect of superoxide dismutase in cats with cold-induced edema.	we evaluated the potential role of sod, an oxygen free radical scavenger, as a probe to cover the trigger period of injury and the prolonged period of development of edema in vasogenic brain edema. vasogenic brain edema was produced in 34 cats by a standardized cortical freezing lesion. brain edema and the disruption in the bbb were assessed by sg measurement and spread of evans blue by planimetry. detection of superoxide radicals was studied by topical application of nbt within the cranial wind ...	1990	2168663
proton-nuclear magnetic resonance relaxation times in brain edema.	proton relaxation times of protein solutions, bovine brain, and edematous feline brain tissue were studied as a function of water concentration, protein concentration, and temperature. in accordance with the fast proton exchange model for relaxation, a linear relation could be established between r1 and the inverse of the weight fraction of tissue water. this relation also applied to r2 of gray matter and of protein solutions. no straightforward relation with water content was found for r2 of wh ...	1990	2168666
phosphonylmethyl ethers of acyclic nucleoside analogues: inhibitors of hsv-1 induced ribonucleotide reductase.	diphosphates of n-(s)-(3-hydroxy-2-phosphonylmethoxypropyl) and n-(2-phosphonylmethoxyethyl) derivatives of purine and pyrimidine heterocyclic bases inhibit hsv-1 encoded ribonucleotide reductase. of the compounds studied, the most efficient inhibitors of cdp reduction (at 5.1 mumols.l-1) by the hsv-1-encoded enzyme are hpmpapp (ic50 = 0.9 mumols.l-1) and pmeapp (ic50 = 8 mumol.l-1). pmeapp does not inhibit the enzyme isolated from the mutant hsv-1 kos strain pmear which is resistant to pmea at ...	1990	2168691
antiinflammatory properties of the new antirheumatic agent 4-acetylaminophenylacetic acid.	the antiinflammatory activity of 4-acetylaminophenylacetic acid (ms-932) was investigated. ms-932 did not suppress the acute inflammation of carrageenin-induced paw edema in rats or of primary swelling in adjuvant arthritic rats. however, prophylactic treatment with ms-932 inhibited secondary inflammation in adjuvant arthritic rats. ms-932 also restored to normal the weight of the spleen and the serum albumin/globulin ratio of adjuvant arthritic rats. in addition to its prophylactic effect, ms-9 ...	1990	2168705
establishment of ddd/1-mtv-2/mtv-2, nu/nu and ddd/1-mtv-2/mtv-2, nu/+ mice: preliminary characterization in relation to mtv antigen expression and mammary tumorigenesis.	to investigate the effects of the t-cell deprivation on viral mammary tumorigenesis, two double congenic mouse strains of the ddd genetic background, ddd/1-mtv-2/mtv-2, nu/nu and ddd/1-mtv-2/mtv-2, nu/+, were produced by the cross between ddd/1-mtv-2/mtv-2 (ddd-mtv-2) and ddd/1-nu/nu mice, followed by repeated intercross breedings. expression of the mouse mammary tumor virus (mtv)-gp52 antigen was demonstrated in the mammary glands of mice from 14 days on, in both -nu/nu and -nu/+ females. mamma ...	1990	2168807
mu-opioid receptors mediate the inhibitory effect of opioids on dopamine-sensitive adenylate cyclase in primary cultures of rat neostriatal neurons.	the receptors mediating the inhibition of d1 dopamine receptor-stimulated adenylate cyclase by opioids were examined in primary cultures of rat neostriatal neurons. adenylate cyclase activity was dose-dependently increased by the selective d1 dopamine receptor agonist skf 38393 (ec50 = 0.05 microm). this stimulation was fully antagonized by the selective d1 dopamine receptor antagonist sch 23390 (1 microm). skf 38393 (1 microm)-stimulated adenylate cyclase activity was strongly reduced (by almos ...	1990	2168934
low-energy laser irradiation--a new measure for suppression of arachidonic acid metabolism in the optic nerve.	helium-neon low-energy laser (lel) irradiation, known for its therapeutic effects in arthritis, wound healing, and muscular strain, was applied to eyes of rats that had sustained an optic nerve crush injury. crush injury to the optic nerve resulted in a long-lasting in vitro elevation of prostaglandin e2 (pge2) production (1.8-3.9-fold above control values during the 12 day study period). lel irradiation per se had no effect in vitro on pge2 production by the optic nerve; however, lel irradiatio ...	1990	2168952
ebna size polymorphism can be used to trace epstein-barr virus spread within families.	the epstein-barr virus (ebv)-determined nuclear antigens ebna 1, 2, 3, 4, and 6, regularly expressed in ebv-transformed lymphoblastoid cell lines, vary in size among viral strains. we have used this characteristic to trace the spread of the virus within seven families by using an approach called ebnotyping. among 33 evaluable individuals, 3 were ebv seronegative, and 17 different ebv strains could be isolated from the peripheral blood or throat washes of the remaining 30. all unrelated persons c ...	1990	2168960
pertussis toxin b-subunit-induced ca2(+)-fluxes in jurkat human lymphoma cells: the action of long-term pre-treatment with cholera and pertussis holotoxins.	the exotoxins of bordetella pertussis and vibrio cholera have been used to investigate signal transduction in the human t-cell lymphoma jurkat. stimulation of the cells, leading to an increase in cytoplasmic free calcium, could be achieved by the anti-t-cell receptor complex antibody okt3 and by pertussis holotoxin (ptht), or its b-subunit (ptb), but not by cholera holotoxin (ctht) or its b-subunit (ctb). both holotoxins adp-ribosylated specifically g-proteins in the plasma membrane of intact ce ...	1990	2169284
photoprotective effect of superoxide-scavenging antioxidants against ultraviolet radiation-induced chronic skin damage in the hairless mouse.	albino hairless mice (skh:hr-1) exposed chronically to suberythemal doses of ultraviolet radiation develop visible skin changes, histological alterations, and tumors. topical treatment of mice with solutions of superoxide-scavenging antioxidants (such as alpha-tocopherol, ascorbic acid, propyl gallate and trolox) prior to each uvb radiation exposure reduced significantly the severity of these events. tocopherol esters and ascorbyl palmitate were not as effective as the parent compounds in provid ...	1990	2169296
effects of a novel 21-amino steroid, u74006f, on the rat pituitary-adrenocortical axis.	the 21-amino steroid u74006f is a potent inhibitor of lipid peroxidation and has been shown to affect beneficially the acutely injured central nervous system. therapeutically, it is desirable for this compound to be devoid of steroid side-effects. we have demonstrated a significant (p less than 0.001) inhibition of basal acth secretion from cultured rat pituitary cells during a 24-h incubation at concentrations (10-100 mumols/l) previously demonstrated to inhibit lipid peroxidation in vitro. u74 ...	1990	2169520
osteoblasts display receptors for and responses to leukemia-inhibitory factor.	specific binding of leukemia-inhibitory factor (lif) to osteoblasts, but not multinucleated osteoclasts, was demonstrated by receptor autoradiography by using cells isolated from newborn rat long bones. the clonal rat osteogenic sarcoma cells, umr 106-06, which have several phenotypic properties of osteoblasts, expressed 300 lif receptors per cell, with an apparent kd of 60 pm. treatment of calvarial osteoblasts or umr 106-01 cells with lif resulted in a dose-dependent inhibition of plasminogen ...	1990	2170427
evidence for a role of the na+/h+ exchanger in the colony-stimulating-factor-induced ornithine decarboxylase activity and proliferation of the human cell line m-07e.	the subclone m-07e, derived from the interleukin-3 (il-3)-responsive human myeloid cell line m-07, is strictly dependent on either il-3 or granulocyte-macrophage-colony-stimulating factor (gm-csf) for its growth and survival. this cell line may be regarded as a candidate model to investigate the poorly understood events triggered by growth factors binding to human hemopoietic cells. both il-3 and gm-csf induce in m-07e cells an increase of ornithine decarboxylase (odc) activity, which reaches it ...	1990	2170428
cytomegalovirus-induced pneumonitis and myocarditis in newborn mice. a model for perinatal human cytomegalovirus infection.	genetically determined resistance to the lethal effects of infection with murine cytomegalovirus (mcmv) has been reported previously in adult and newborn mice. we examined the pathogenesis of mcmv infection in resistant (cba, h-2k) and susceptible (balb/c, h-2d) mice infected intraperitoneally on the day of birth. balb/c mice developed a severe interstitial pneumonitis and myocarditis 10 days post-infection. their pulmonary and tissues contained high mcmv titres and large numbers of mcmv-antigen ...	1990	2174234
a nontraditional role for water in the cytochrome c oxidase reaction.	the passage of electrons through cytochrome c oxidase is directly related to the activity of water. reducing the activity in a system containing reductant, oxygen, and cytochrome oxidase blocks electron transfer between reduced cytochrome a and oxidized cytochrome a3. the extent of the block is directly related to the osmotic pressure of the system, implying that the protein shell of the oxidase acts as a semipermeable membrane that excludes osmotic perturbants but not water. it appears that app ...	1990	2174258
two-step processing for activation of the cytolysin/hemolysin of vibrio cholerae o1 biotype el tor: nucleotide sequence of the structural gene (hlya) and characterization of the processed products.	vibrio cholerae o1 biotype el tor produces and secretes a 65-kda cytolysin/hemolysin into the culture medium. we cloned the structural gene (hlya) for the cytolysin from the total dna of a v. cholerae o1 el tor strain, n86. nucleotide sequence analysis of hlya revealed an open reading frame consisting of 2,223 bp which can code for a protein of 741 amino acids with a molecular weight of 81,961. consistent with this, a 79-kda protein was identified as the product of hlya by maxicell analysis in e ...	1990	2174833
transcriptional regulation by iron of a vibrio cholerae virulence gene and homology of the gene to the escherichia coli fur system.	we have previously described an iron-regulated virulence determinant in vibrio cholerae. strain mbg40, which contains a tnphoa insertion mutation in the iron-regulated gene irga, has reduced virulence in a newborn mouse model and has lost the major 77-kda iron-regulated outer membrane protein. we report here the cloning of the irga'-'phoa gene fusion, the sequencing of the 5'-proximal portion of irga, and the definition of its promoter region by primer extension. the deduced amino acid sequence ...	1990	2174861
a new selective, differential agar medium for isolation of vibrio cholerae o1: pmt (polymyxin-mannose-tellurite) agar.	a new selective and differential agar medium, polymyxin-mannose-tellurite (pmt) agar was devised to differentiate easily colonies of vibrio cholerae o1 from those of v. cholerae non-o1. the differentiation between colonies of the two vibrios is based on mannose-fermentation. colonies of v. cholerae o1 on the agar are agglutinated with o1 antiserum of v. cholerae much more easily than those on thiosulfate-citrate-bile salts-sucrose (tcbs) agar.	1990	2175000
[cholestyramine adsorbs escherichia coli and vibrio cholerae toxins by way of ion exchange mechanism].	the use of cholestyramine in the treatment of enterocolitis is justified by its ability to fix the lt toxin of vibrio cholerae and etec, the st toxin of etec and the verotoxin of ehec at the ph of intestinal fluid. to elucidate the nature of the ion-exchange mechanism in the binding of the toxins on the resin, we try to eluate the toxins with 0.1 m to 0.9 m nacl solution at ph 7. irrespective of their presence or absence in the different fractions, the biological activity of the toxins was asses ...	1990	2181919
high- and low-density lipoproteins enhance infection of trypanosoma cruzi in vitro.	trypanosoma cruzi exhibits a developmentally regulated neuraminidase activity that is inhibited by high-density lipoprotein (hdl). we report here that the infection of culture cells by t. cruzi trypomastigotes is enhanced by hdl in a dose-dependent manner. the enhanced infection is prevented by vibrio cholerae neuraminidase, an enzyme whose activity is not inhibited by hdl, suggesting that sialic acid is involved in t. cruzi-host interaction. similar enhancement of infection is also produced by ...	1990	2183047
diabetes mellitus, the renin-angiotensin system, and angiotensin-converting enzyme inhibition.	many traditional antihypertensive drugs are linked to various effects that may cause concern in the hypertensive diabetic patient. the favorable tolerability of angiotensin-converting enzyme (ace) inhibitors in essential hypertension makes it likely that they will be well tolerated when used in diabetic patients. data from ongoing studies support the theory that ace inhibitors lower blood pressure to the same extent in hypertensive diabetics as in patients with essential hypertension. ace inhibi ...	1990	2189073
construction of a bivalent oral vaccine for prevention of typhoid fever and cholera diarrhea.	a recombinant plasmid pmm-ctb containing the gene for production of the nontoxic b subunit of vibrio cholera was transferred into a safe, effective and attenuated oral vaccine ty21a strain of salmonella typhi. the resulting ty21a (pmm-ctb) could steadily produce ct-b subunit that was secreted extracellularly and had the same antigenicity as ct-b produced by v. cholera. furthermore, the characteristics of the antigenicity, the persistance in mice and the galactose sensitivity possessed in the str ...	1990	2190570
vibrio cholerae serogroup o1 in northeast thailand.	strains of vibrio cholerae serogroup o1 biotype el tor that are susceptible to mukerjee cholera bacteriophage group iv (s. mukerjee, bull. w.h.o. 28:333-336, 1963) were found. cholera vibrios isolated from epidemics in northeast thailand were characterized, and 57 of 60 strains isolated in 1986 were susceptible to cholera phage iv. however, all 113 strains isolated in 1988 were not susceptible to the phage. all isolates in both epidemics revealed behaviors typical of el tor vibrios, except phage ...	1990	2191009
binding of bacteria to carbohydrates immobilized on beads to demonstrate the presence of cell-associated hemagglutinins in vibrio cholerae.	we describe a phase contrast microscopy method for direct observation of classical and el tor vibrios to agarose beads containing covalently attached l-fucose or d-mannose. binding of the vibrios to l-fucose beads was found to correlate with fucose-sensitive agglutination of human o erythrocytes, while binding of bacteria to beads with d-mannose was consistent with mannose-sensitive agglutination of chicken erythrocytes. furthermore, vibrios expressing both fucose and mannose-sensitive hemagglut ...	1990	2191694
factors influencing semen quality in pigs.		1990	2192031
no acquisition of metastatic capacity of r1h rhabdomyosarcoma upon transfection with c-ha-ras oncogene.	the effect of the activated c-ha-ras oncogene on invasiveness and formation of spontaneous metastases was studied using the rhabdomyosarcoma r1h of the rat. r1h tumor cells which are able to grow in vitro and produce tumors upon subcutaneous injection in syngeneic wag/rij rats were transfected with the c-ha-ras (ej) oncogene and the neomycin gene for selection. two r1h cell lines harboring and expressing the human c-ha-ras oncogene, one cell line containing the neomycin gene only, and the parent ...	1990	2192992
[inhibition of enteropathogens by clostridium butyricum miyairi 588].	the inhibitory effect of clostridium butyricum miyairi 588 against various enteropathogens was investigated in mixed cultures. it was observed that c. butyricum m588 inhibited the growth of vibrio cholerae o1, v. cholerae non-o1, aeromonas hydrophila, and shigella flexneri. considering that the interaction between c. butyricum and shigella is especially important because of their proliferation site in the lower intestine, further examinations were carried out on shigella in particular. results w ...	1990	2193065
hepatic apo b-100 lipoproteins and plasma ldl heterogeneity in african green monkeys.	the contribution of hepatic apolipoprotein (apo) b-100 lipoproteins to plasma low-density lipoprotein (ldl) metabolic heterogeneity was examined in african green monkeys. hepatic 3h-labeled very low-density lipoproteins (vldl) (d less than 1.006, where d is density in g/ml) or hepatic 131i-labeled ldl (1.030 less than d less than 1.063) were isolated from perfused livers and injected simultaneously with autologous plasma 125i-ldl into african green monkeys. serial blood samples were taken, and t ...	1990	2193530
direct effect of parathyroid hormone on insulin secretion from pancreatic islets.	indirect evidence indicates that parathyroid hormone (pth) interacts with pancreatic islets and modulates their insulin secretion. this property of pth has been implicated in the genesis of impaired insulin release in chronic renal failure. we examined the direct effect of pth-(1-84) and pth-(1-34) on insulin release using in vitro static incubation and dynamic perifusion of pancreatic islets from normal rats. both moieties of the hormone stimulated in a dose-dependent manner glucose-induced ins ...	1990	2193536