Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| effect of exogenous growth-hormone-releasing factor on blood metabolites and minerals in late maturing buffalo heifers (bubalus bubalis). | previous studies have suggested that growth-hormone-releasing factor (grf) enhanced growth and advanced puberty onset along with hormonal changes in buffalo heifers (bubalus bubalis). however, it is not known to what extent exogenous grf could influence blood metabolites and minerals to bring about puberty in buffalo heifers. therefore, we planned to investigate the effect of exogenous bovine grf (bgrf) on blood metabolites and minerals in buffalo heifers during a 3-month pre-treatment period, 9 ... | 2007 | 17615004 |
| capacitation of frozen thawed buffalo bull (bubalus bubalis) spermatozoa with higher heparin concentrations. | the present study was conducted to examine the effect of high heparin concentration on capacitation of buffalo spermatozoa with a short incubation time. frozen thawed spermatozoa from three buffalo bulls were pooled and treated with either 50, 100 or 200 microg/ml heparin for 30 min. capacitation was evaluated by acrosome reaction of spermatozoa and in vitro fertilization rate (per cent cleavage rate, per cent cleavage index). acrosome reaction was induced in heparin treated spermatozoa with cal ... | 2007 | 17635774 |
| water buffaloes (bubalus bubalis) identified as an important reservoir of shiga toxin-producing escherichia coli in brazil. | the presence of shiga toxin-producing escherichia coli (stec) in water buffaloes is reported for the first time in south america. the prevalence of stec ranged from 0 to 64% depending on the farm. stec isolates exhibiting the genetic profiles stx(1)stx(2)ehxa iha saa and stx(2)ehxa iha saa predominated. of the 20 distinct serotypes identified, more than 50% corresponded to serotypes associated with human diseases. | 2007 | 17644639 |
| comparison of two doses of ropivacaine for lumbosacral epidural analgesia in buffalo calves (bubalus bubalis). | six male buffalo calves aged from six to eight months and weighing 100 to 120 kg were used to evaluate the efficacy and safety of caudal epidural analgesia produced by doses of 37.5 mg (5 ml) and 75 mg (10 ml) ropivacaine (0.75 per cent). the study was completed in two phases. in phase 1, all the animals were used twice to evaluate the analgesic and clinical effects of two doses of ropivacaine. in phase 2, the animals were divided into two groups of three to evaluate the effects of two doses of ... | 2007 | 17545647 |
| single-dose pharmacokinetics of marbofloxacin in egyptian buffalo (bubalus bubalis l.) steers. | the objective of this study was to investigate the pharmacokinetics of marbofloxacin (mar) following intravenous (iv) and intramuscular (im) administration of a 2.0 mg/kg body weight dosage to five healthy egyptian buffalo steers. a cross-over design was used with a washout period of 2 weeks. blood samples were obtained at 0, 5,10,15, and 20 min and at 0.5,0.75,1,2,4,6,8,10,12,24,30 and 48 hours after marbofloxacin administration. the serum marbofloxacin concentrations were quantitated using a m ... | 2007 | 17555042 |
| preliminary radiation hybrid map for river buffalo chromosome 6 and comparison to bovine chromosome 3. | we present the first radiation hybrid (rh) map of river buffalo (bubalus bubalis) chromosome 6 (bbu6) developed with a recently constructed river buffalo whole-genome rh panel (bburh(5000)). the preliminary map contains 33 cattle-derived markers, including 12 microsatellites, 19 coding genes and two ests, distributed across two linkage groups. retention frequencies for markers ranged from 14.4% to 40.0%. most of the marker orders within the linkage groups on bbu6 were consistent with the cattle ... | 2007 | 17559550 |
| phylogenetic relationships and status quo of colonies for gayal based on analysis of cytochrome b gene partial sequences. | thirty-three mutations and four different haplotypes were found when cytochrome b(cytb) gene partial sequences of 12 gayals were analyzed. together with sequences of bos indicus, bos taurus, bos grunniens and bos gaurus with bubalus bubalis as the out group, the partial sequences of cytb gene of gayals were aligned and base composition and nucleotide variation of cytb gene were analyzed. the phylogenetic trees were constructed by the nj method and the mp method respectively, both supporting almo ... | 2007 | 17560527 |
| biometry of frozen-thawed sperm from eight breeds of indian buffaloes (bubalus bubalis). | sperm morphometry, in combination with other objective traits, can be useful for developing a fertility index. the objective of the present study was to measure various biometric end points of frozen-thawed sperm from eight breeds of indian buffaloes (murrah, surti, tarai, mehsana, jaffrabadi, bhadawari, pandharpuri and nili-ravi). the sperm head of pandharpuri buffaloes had the greatest length (10.21 microm), width (6.05 microm), area (52.31 microm(2)) and perimeter (31.86 microm). the ratio of ... | 2007 | 17573105 |
| morphological features of spermatozoa of swamp buffalo ai bulls in thailand. | the appearance and incidence of sperm abnormalities was studied in 115 ejaculates, collected periodically over 1 year covering all seasons from five mature, healthy swamp buffalo (bubalus bubalis) bulls reared under tropical conditions and serving as the current source of semen for artificial insemination (ai) in thailand. light microscopy of stained smears was used to investigate sperm head shape morphology, while unstained wet smears were used to examine other sperm abnormalities. the most com ... | 2007 | 17493162 |
| low incidence of bovine leukocyte adhesion deficiency (blad) carriers in indian cattle and buffalo breeds. | blad is an autosomal recessive genetic disease that affects holstein-friesian (hf) cattle worldwide. it is a disease characterized by a reduced expression of the adhesion molecules on neutrophils. the disease is caused by a mutation that replaces adenine at 383 with guanine, which causes an amino acid change from aspartic acid to glycine. blood samples and a few semen samples were collected from 1250 phenotypically normal individuals, including hf (n=377), hf crossbred (n=334), jersey (105), oth ... | 2007 | 17495349 |
| cloning, sequencing and expression of cdna of bovine neutrophil beta-defensin from water buffalo (bubalus bubalis). | neutrophil beta-defensins have been identified as naturally occurring potent antibacterial cationic peptides serving as effector molecules of innate immunity that provide a first line of defence against pathogens. considering the broad-spectrum antimicrobial activity against microorganisms and role in innate immunity of the neutrophil beta-defensins, it has been characterized in many livestock species including cattle, sheep, caprine and porcines. here we report the isolation, cloning, sequencin ... | 2007 | 17504507 |
| ovarian dynamics and milk progesterone concentrations in cycling and non-cycling buffalo-cows (bubalus bubalis) during ovsynch program. | the objective was to evaluate ovarian dynamics and progesterone concentrations in cyclic (cyc, n=10) and non-cyclic (ncy, n=8) buffalo-cows during ovsynch program. all cows received gnrh on day 0, pgf2alpha on day 7, and gnrh on day 9, and ai 14 h later. ovarian structures were monitored by ultrasound and milk samples were collected for progesterone (p4) analysis. the first gnrh resulted in ovulation in cyc (90%) and ncy (62.5%) cows. by day 7, almost all cows had large follicle and lutein tissu ... | 2007 | 17482252 |
| bubaline versus bovine reproduction. | fertility in water buffalo (bubalus bubalis) is considerably lower than that in cattle (bos taurus and bos indicus). poor breeding efficiency is attributed to late onset of puberty, seasonality, poor estrus expression, and long calving intervals. accurate estrus detection is a prerequisite for efficient reproductive management. established reproductive management techniques in cattle can be successfully applied to water buffalo because of the similarities in the anatomy, physiology, and endocrin ... | 2007 | 17482253 |
| real-time taqman polymerase chain reaction detection and quantification of cow dna in pure water buffalo mozzarella cheese: method validation and its application on commercial samples. | mozzarella cheese obtained from buffalo (bubalus bubalis) milk is a typical italian product certificated by means of the european protected designation of origin (pdo). mozzarella cheese can also be obtained from bovine milk or bovine/buffalo milk mixtures, but in this case, it cannot be sold as pdo product, and its label must report the actual ingredients. however, bovine milk in pdo products was frequently detected in the past, suggesting fraudulent addition or accidental contamination. severa ... | 2007 | 17419643 |
| clinical, haematological and therapeutic studies on tropical theileriosis in water buffaloes (bubalus bubalis) in egypt. | thirty buffaloes naturally infected with theileria annulata and 10 parasitologically free controls were used to determine the potential clinical, haematological and therapeutic impact of tropical theileriosis in egypt. the clinical signs in the infected buffaloes were pyrexia (40.5-41.5 degrees c), enlargement of superficial lymph nodes, slight nasal and ocular discharges, salivation, anaemia and respiratory distress. eye lesions also were recorded. there was a significant decrease in erythrocyt ... | 2007 | 17420101 |
| seasonality affects post-thaw plasma membrane intactness and sperm velocities in spermatozoa from thai ai swamp buffaloes (bubalus bubalis). | altogether 218 frozen semen ai doses, prepared between 1980 and 1989 and also between 2003 and 2005 from 18 ai thai swamp buffalo sires, were examined to determine whether seasonality affects post-thaw viability, as plasma membrane integrity (pmi, using sybr-14/pi), plasma membrane stability (pms, using annexin-v/pi), or motility (mot, using casa). a thermoresistance test (38 degrees c for 60 min) was used to further analyze sperm survivability in vitro. all variables were compared over 3 season ... | 2007 | 17442385 |
| isolation and sequence characterization of mammary derived growth inhibitor gene of riverine buffalo (bubalus bubalis). | in this study, attempts have been made to identify and characterize water buffalo (bubalus bubalis) mammary derived growth inhibitor (mdgi) gene, isolated from a mammary gland cdna library of lactating buffalo. the complete mdgi cdna was of 698 nucleotides, consisting 61 nucleotides in 5' utr, coding region of 402 nucleotides, and 235 nucleotides representing the 3' utr. comparison of nucleotide and deduced amino acid sequence data with that of mdgi/fatty acid binding protein (fabp) of other spe ... | 2007 | 17453652 |
| mitochondrial dna analyses of indian water buffalo support a distinct genetic origin of river and swamp buffalo. | water buffalo (bubalus bubalis) is broadly classified into river and swamp categories, but it remains disputed whether these two types were independently domesticated, or if they are the result of a single domestication event. in this study, we sequenced the mitochondrial d-loop region and cytochrome b gene of 217 and 80 buffalo respectively from eight breeds/locations in northern, north-western, central and southern india and compared our results with published mediterranean and swamp buffalo s ... | 2007 | 17459014 |
| sequence variation and molecular evolution of hormone-sensitive lipase genes in species of bovidae. | the partial sequences of exon i of hormone-sensitive lipase (hsl) genes in yak (bos grunniens), cattle (bos taurus), zebu (bos indicus), and buffalo (bubalus bubalis) were analyzed. comparisons of these sequences and the deduced amino acid sequences with the homologous hsl gene and protein sequences in other mammalian species including pig (sus scrofa), human (homo sapiens), mouse (mus musculus), and rat (rattus sp.) retrieved from the genbank were carried out and finally a phylogenetic tree was ... | 2007 | 17469775 |
| comparative pharmacokinetics of sulfamethazine after intravenous administration in bovine (bos taurus) and buffalo (bubalis bubalis) calves. | 2007 | 17472661 | |
| buffalos (bubalus bubalis) cloned by nuclear transfer of somatic cells. | cloning of buffalos (bubalus bubalis) through nuclear transfer is a potential alternative approach in genetic improvement of buffalos. however, to our knowledge, cloned offspring of buffalos derived from embryonic, fetal, or somatic cells have not yet been reported. thus, factors affecting the nuclear transfer of buffalo somatic cells were examined, and the possibility of cloning buffalos was explored in the present study. treatment of buffalo fibroblasts and granulosa cells with aphidicolin plu ... | 2007 | 17475931 |
| advanced reproductive technology in the water buffalo. | embryo transfer techniques in water buffalo were derived from those in cattle. however, the success rate is much lower in buffaloes, due to their inherent lower fertility and poor superovulatory response. the buffalo ovary has a smaller population of recruitable follicles at any given time than the ovary of the cow (89% fewer at birth). in addition, estrus detection is problematic. progress in the field of embryo transfer in water buffalo has been slow, and is primarily due to a poor response to ... | 2007 | 17481722 |
| congenital ocular dermoid cyst in a river buffalo (bubalus bubalis) calf. | clinical and histopathological findings of a congenital ocular dermoid cyst, located at the lower eyelid of a river female buffalo (bubalus bubalis) calf were presented. a soft, fluctuant, non-tender, hyperaemic cystic mass was detected overlaying the left eye. fine needle aspirate revealed filamentous debris with no malignant cells. the cyst was treated surgically by orbital exenteration and subsequently subjected to histopathological examination. the histopathological study disclosed a conjunc ... | 2007 | 17359456 |
| molecular cloning, characterization, and expression studies of water buffalo (bubalus bubalis) somatotropin. | cloning, high-level expression, and characterization of the somatotropin (st) gene of an indigenous nili-ravi breed of water buffalo bubalus bubalis (bbst) are described. coding, non-coding, and promoter regions of bbst were amplified and sequenced. sequence analysis revealed several silent and two interesting point mutations on comparison with sts of other vertebrate species. one interesting variation in the bbst sequence was the replacement of a conserved glutamine residue by arginine. a plasm ... | 2007 | 17367293 |
| construction of a river buffalo (bubalus bubalis) whole-genome radiation hybrid panel and preliminary rh mapping of chromosomes 3 and 10. | the buffalo (bubalus bubalis) is a source of milk and meat, and also serves as a draft animal. in this study, a 5000-rad whole-genome radiation hybrid (rh) panel for river buffalo was constructed and used to build preliminary rh maps for bbu3 and bbu10 chromosomes. the preliminary maps contain 66 markers, including coding genes, cattle expressed sequence tags (ests) and microsatellite loci. the rh maps presented here are the starting point for mapping additional loci that will allow detailed com ... | 2007 | 17403053 |
| milk of cow (bos taurus), buffalo (bubalus bubalis), and goat (capra hircus): a better alternative than fetal bovine serum in media for primary isolation, in vitro cultivation, and maintenance of leishmania donovani promastigotes. | tyndalized milk of goat, cow, and buffalo was found to be a potential substitute for fetal bovine serum (fbs) in the medium for the cultivation of leishmania donovani promastigotes. the numbers (means) of promastigotes reached 2.6 x 10(7), 2.3 x 10(7), and 2.1 x 10(7)/ml, respectively, in the medium supplemented with 10% milk of goat, cow, and buffalo, in comparison to 1.9 x 10(7)/ml in the control with 10% fbs. in primary isolation, the milk-supplemented medium showed that 22 out of 26 samples ... | 2007 | 17287321 |
| characterization and multilineage differentiation of embryonic stem cells derived from a buffalo parthenogenetic embryo. | embryonic stem (es) cells derived from mammalian embryos have the ability to form any terminally differentiated cell of the body. we herein describe production of parthenogenetic buffalo (bubalus bubalis) blastocysts and subsequent isolation of an es cell line. established parthenogenetic es (pges) cells exhibited diploid karyotype and high telomerase activity. pges cells showed remarkable long-term proliferative capacity providing the possibility for unlimited expansion in culture. furthermore, ... | 2007 | 17290421 |
| blood biochemical and ruminal liquor profile in buffaloes (bubalus bubalis) showing omasal impaction. | the blood biochemical and ruminal fluid parameters of 5 buffaloes showing omasal impaction were studied, together with 10 healthy buffaloes as control. the diseased buffaloes had significantly higher serum aspartate aminotransferase, bilirubin, glucose, blood urea nitrogen, creatinine, total protein, globulin and fibrinogen levels and significantly lower plasma calcium, potassium and chloride concentrations than the controls. the ruminal liquor of the diseased buffaloes revealed characteristic p ... | 2007 | 17294261 |
| genetic traceability of the geographical origin of typical italian water buffalo mozzarella cheese: a preliminary approach. | to distinguish italian protected designation of origin (pdo) water buffalo mozzarella from different producers on a molecular basis in relation to the place of manufacturing within the production district, and to develop a tool for genetic traceability of typical dairy products. | 2007 | 17309615 |
| independent maternal origin of chinese swamp buffalo (bubalus bubalis). | to obtain more knowledge on the origin and genetic diversity of the swamp buffalo (bubalus bubalis) in china, the complete mitochondrial d-loop sequences of 119 samples representing seven native types were compared. two mitochondrial dna (mtdna) lineages (lineages a and b) were determined for the chinese swamp buffalo. examination of the diversity patterns suggest that lineage a has undergone a population expansion event. divergence of lineages a and b was estimated at 18,000 years ago. combined ... | 2007 | 17313588 |
| differentiating human bone from animal bone: a review of histological methods. | this review brings together a complex and extensive literature to address the question of whether it is possible to distinguish human from nonhuman bone using the histological appearance of cortical bone. the mammalian species included are rat, hare, badger, racoon dog, cat, dog, pig, cow, goat, sheep, deer, horse, water buffalo, bear, nonhuman primates, and human and are therefore not exhaustive, but cover those mammals that may contribute to a north american or eurasian forensic assemblage. th ... | 2007 | 17316219 |
| the nramp1aa genotype confers susceptibility to brucella abortus in water buffalo. | the 3' untranslated region of the water buffalo nramp1 (natural resistance-associated macrophage protein 1) gene contains two alleles (nramp1a and nramp1b), as detected by the denaturing gradient gel electrophoresis (dgge) technique. the nramp1bb genotype is associated with resistance of water buffalo to the intracellular pathogen brucella abortus. this article provides evidence that the nramp1aa genotype is associated with susceptibility to the same pathogen. susceptibility or resistance of wat ... | 2007 | 17334658 |
| qualitative and quantitative studies of the inferior olivary complex in the water buffalo (buballus bubalis). | the shape and neuronal number of the inferior olivary complex (ioc) were determined in the water buffalo (buballus bubalis). the configuration and interrelations of the ioc compartments were ascertained by investigating serial sections through the whole rostro-caudal extent of the ioc. nissl-stained celloidin sections of six water buffalo's brainstems were used. the ioc in the water buffalo consisted of three major nuclei and four small cell groups. the medial accessory olivary nucleus (mao) had ... | 2007 | 17339758 |
| development of a competitive quantitative pcr strategy for evaluating the expression stability of 18s rrna during in vitro maturation of buffalo (bubalus bubalis) follicular oocytes. | the present work describes the development of a quantitative competitive pcr strategy for quantifying the relative abundance of 18s rrna transcripts in buffalo oocytes during in vitro maturation (ivm). as a method, the competitive pcr overcomes some of the shortcomings of conventional reverse transcriptase polymerase chain reaction (rt-pcr) procedure making it a more authentic quantitative method. a composite primer based approach was used to generate the competitor cdna to be used as external c ... | 2007 | 17348978 |
| effects of tiamulin, neomycin, tetracycline, fluorophenicol, penicillin g, linco-spectin, erythromycin and oxytetracycline on controlling bacterial contaminations of the river buffalo (buballus bubalis) semen. | in order to investigate the effects of tiamulin, neomycin, tetracycline, fluorophenicol, penicillin g, linco-spectin (0.15 mg ml(-1) lincomycin + 0.3 mg ml(-1) spectinomycin), erythromycin and oxytetracycline on controlling bacterial contaminations of the river buffalo semen, 120 ml diluted buffalo bull semen (diluted by tris-egg yolk extender) was divided into 5 ml tubes after initial evaluation and before (control sample) and at 0, 2, 6, 12 and 24 h after adding each of the above antibiotics a ... | 2007 | 19090126 |
| cleft palate in a male water buffalo calf. | congenital palatal defects are common in animals but there is only one report of water buffalo has been recorded in iran. one died male water buffalo calf was examined after hysterotomy operation. at necropsy findings, brachygnathia, palate cleft and small lungs were diagnosed. it is the second report of water buffalo cleft palate in iran. | 2007 | 19093537 |
| geo-referencing livestock farms as tool for studying cystic echinococcosis epidemiology in cattle and water buffaloes from southern italy. | cystic echinococcosis (ce), caused by the larval stages of the tapeworm echinococcus granulosus, is known to be one of the most important parasitic infection in livestock worldwide and one of the most widespread zoonoses known. in the present study, we used a geographical information system (gis) to study the spatial structure of livestock (cattle, water buffaloes and sheep) populations to gain a better understanding of the role of sheep as reservoir for the transmission of ce to cattle and wate ... | 2007 | 18686260 |
| cytosolic and microsomal glutathione-s-transferases from bovine filarial worms setaria cervi. | the work presented here deals with the status of glutathione-s-transferase (gst; e.c. 2.5.1.18), the major enzyme of the phase ii detoxification pathway, in bovine filarial worms setaria cervi. gst activity was determined in various subcellular fractions of bovine filarial worms s. cervi (bubalus bubalis linn.) and was found to be mainly associated with cytosolic and microsomal fractions. the respective specific activities of the enzyme from cytosolic and microsomal fractions of s. cervi females ... | 2007 | 18314670 |
| a radiation hybrid map of river buffalo (bubalus bubalis) chromosome 1 (bbu1). | the largest chromosome in the river buffalo karyotype, bbu1, is a submetacentric chromosome with reported homology between bbu1q and bovine chromosome 1 and between bbu1p and bta27. we present the first radiation hybrid map of this chromosome containing 69 cattle derived markers including 48 coding genes, 17 microsatellites and four ests distributed in two linkage groups spanning a total length of 1330.1 cr(5000). the rh map was constructed based on analysis of a recently developed river buffalo ... | 2007 | 18160788 |
| characterization of smoc-1 uncovers two transcript variants showing differential tissue and age specific expression in bubalus bubalis. | secreted modular calcium binding protein-1 (smoc-1) belongs to the bm-40 family which has been implicated with tissue remodeling, angiogenesis and bone mineralization. besides its anticipated role in embryogenesis, smoc-1 has been characterized only in a few mammalian species. we made use of the consensus sequence (5' cacctctccacctgcc 3') of 33.15 repeat loci to explore the buffalo transcriptome and uncovered the smoc-1 transcript tagged with this repeat. the main objective of this study was to ... | 2007 | 18042303 |
| ultrastructural and morphometric characterization of buffalo (bubalus bubalis) ovarian preantral follicles. | the main objective of the present study was to characterize buffalo preantral ovarian follicles. parts of ovarian cortex, collected from postpubertal buffalo females that were having estrous cycles at regular intervals, were selected under stereomicroscopy and processed for optic and transmission electron microscopy. primordial follicles were characterized as an oocyte encircled by one layer of flattened cells. the buffalo primordial follicle has a mean diameter of 35 microm and the oocyte diame ... | 2007 | 16580155 |
| effect of bradykinin on murrah buffalo (bubalus bubalis) semen cryopreservation. | keeping in view the poor freezability of bubaline semen in conventionally used extenders, this study was conducted on three murrah bulls to improve semen cryopreservation with the incorporation of bradykinin (0.5, 1.0 and 2.0 ng ml(-1)) in routinely used egg yolk tris-glycerol (eytg) extender. bradykinin (2.0 ng ml(-1)) had significant (p<0.05) beneficial effect on live sperm % (81.6+/-1.8) and hypo osmotic swelling (hos) % (63.0+/-1.3) as compared to their respective control values of 73.4+/-2. ... | 2007 | 16600533 |
| cryopreservation of in vitro matured buffalo (bubalus bubalis) oocytes by minimum volumes vitrification methods. | the aim of this study was to evaluate the efficiency of the solid surface vitrification (ssv) and the cryoloop vitrification (clv) methods to cryopreserve in vitro matured buffalo oocytes. another objective of the work was to investigate whether the presence of cumulus cells affects the efficiency of oocyte vitrification in this species. in the ssv method, oocytes were vitrified in a solution of 35% ethylene glycol, 5% polyvinyl-pyrrolidone and 0.4% trehalose and they were warmed in a 0.3m treha ... | 2007 | 16698202 |
| complete sequence of the yak (bos grunniens) mitochondrial genome and its evolutionary relationship with other ruminants. | the yak (bos grunniens) is the most important domesticated species in the qinhai-tibetan plateau. in present study, the complete sequence of the yak mitochondrial genome was determined. sequence analysis revealed that there are no differences with cattle in the yak mitochondrial genome organization. interestingly, within the d-loop, the conserved sequence blocks are less conserved than surrounding regions. neighbor-joining (nj) trees based on single genes, gene sets and concatenated genes of mit ... | 2007 | 16942892 |
| seroepidemiology of neospora caninum and toxoplasma gondii in cattle and water buffaloes (bubalus bubalis) in the people's republic of china. | a seroepidemiological survey of neospora caninum and toxoplasma gondii in cattle and water buffaloes was carried out in the people's republic of china. serum samples were obtained from dairy (n=262, 9 herds in 9 provinces) and beef cattle (n=10, 1 herd) and water buffaloes (n=40) in china. all sera were tested for antibodies to n. caninum and t. gondii by an enzyme-linked immunosorbent assay (elisa) and an indirect agglutination test (iat), respectively. the overall seroprevalence of n. caninum ... | 2007 | 17010521 |
| vegf system expression in different stages of estrous cycle in the corpus luteum of non-treated and superovulated water buffalo. | water buffaloes are easily adaptable animals, whose raising and economical exploitation have been growing in the last three decades all over the world. hyperstimulation of ovarian function in this species is a common technique aiming to improve reproductive performance. superovulatory treatment affects corpus luteum (cl) function, which is highly correlated to angiogenic process. the aim of this study was therefore to assess the temporal protein and mrna expression of vegf and its receptors in t ... | 2007 | 17014980 |
| isolation and characterization of embryonic stem cell-like cells from in vitro-produced buffalo (bubalus bubalis) embryos. | this study was carried out to isolate and characterize buffalo embryonic stem (es) cell-like cells from in vitro-produced embryos. inner cell mass (icm) cells were isolated either mechanically or by enzymatic digestion from 120 blastocysts whereas 28 morulae were used for the isolation of blastomeres mechanically. the icm cells/ blastomeres were cultured on mitomycin-c-treated feeder layer. primary cell colony formation was higher (p < 0.05) for hatched blastocysts (73.1%, 30/41) than that for e ... | 2007 | 17034054 |
| birth of twins after in vitro fertilization with flow-cytometric sorted buffalo (bubalus bubalis) sperm. | flow-cytometric sorting of mammalian sperm for production of offspring with the desired sex is one of the most important new biotechnologies available for livestock industry. the objectives of this study were: (i) to sort the sperm into x- and y-enriched populations and (ii) use the sorted sperm for in vitro fertilization (ivf) to produce sex-preselected embryo and offspring. the results revealed that the accuracy of sorted x- and y-sperm was 94% and 89%, respectively. there was a decrease in bl ... | 2007 | 17074453 |
| protocols for synchronizing estrus and ovulation in buffalo (bubalus bubalis): a review. | poor estrus expression and a prolonged intercalving interval compromise the reproductive efficiency of female buffaloes. these limitations are exacerbated during the hot season, when fertility decreases dramatically. pregnancy rate decrease further because difficulties in detecting estrus. to improve reproductive efficiency, several protocols of estrus and ovulation synchronization have been developed. these procedures are based on manipulating the cl, either to induce premature luteolysis using ... | 2007 | 17112577 |
| genetic variation of foot-and-mouth disease virus isolates recovered from persistently infected water buffalo (bubalus bubalis). | genetic variation of foot-and-mouth disease virus (fmdv) isolates, serotype o, recovered serially over a 1-year period from persistently infected buffalos was assessed. the persistent state was established experimentally with plaque-purified fmdv, strain o(1)campos, in five buffalos (bubalus bubalis). viral isolates collected from esophageal-pharyngeal (ep) fluids for up to 71 weeks after infection were analyzed at different times by nucleotide sequencing and t(1) rnase oligonucleotide fingerpri ... | 2007 | 17113729 |
| effects of growth hormone-releasing factor on growth hormone response, growth and feed conversion efficiency in buffalo heifers (bubalus bubalis). | the aim of this study was to determine the benefits of growth hormone-releasing factor (grf) on growth and feed conversion efficiency (fce) in buffaloes. twelve murrah buffalo heifers (bubalus bubalis) of mean age 24.8 months and mean body weight 302.4kg were divided into two groups (treatment and control) with six animals in each group. the buffaloes were given intravenous injections of bovine grf (bgrf) at a dose rate of 10microg/100kg body weight or an equal volume of saline at 15-day interva ... | 2007 | 17113797 |
| molecular characterization of coding sequences and analysis of toll-like receptor 3 mrna expression in water buffalo (bubalus bubalis) and nilgai (boselaphus tragocamelus). | toll-like receptor 3 (tlr3), an antiviral innate immunity receptor recognizes double-stranded rna, preferably of viral origin and induces type i interferon production, which causes maturation of phagocytes and subsequent release of chemical mediators from phagocytes against some viral infections. the present study has characterized tlr3 complementary dna (cdna) in buffalo (bubalus bubalis) and nilgai (boselaphus tragocamelus). tlr3 coding sequences of both buffalo and nilgai were amplified from ... | 2007 | 17119952 |
| bovine and water buffalo mx2 genes: polymorphism and antiviral activity. | millennia-long selective pressure of single-strand rna viruses on the bovine mx locus has increased the advantages of using the bovine mx protein to evaluate the ultimate significance of the antiviral role of mx proteins. the conclusions of research based only on the bovine mx1 protein showed the need for comprehensive studies that demonstrate the role of all isoforms, individually or together, especially in the presence of a second isoform, the bovine mx2 gene. this study provides information a ... | 2007 | 17119954 |
| protective effect of the nramp1 bb genotype against brucella abortus in the water buffalo (bubalus bubalis). | we tested 413 water buffalo cows (142 cases and 271 controls) for the presence of anti-brucella abortus antibodies (by the skin test, the agglutination test, and the complement fixation test) and the nramp1 genotype (by capillary electrophoresis). four alleles (nramp1a, -b, -c, and -d) were detected in the 3' untranslated region of the nramp1 gene. the bb genotype was represented among only controls, providing evidence that this genotype confers resistance to brucella abortus. the monocytes from ... | 2007 | 17145946 |
| seasonal variation in semen quality of swamp buffalo bulls (bubalus bubalis) in thailand. | to test the hypothesis that season affects the semen quality of swamp buffalo (bubalus bubalis) bulls used for artificial insemination (ai) under tropical conditions in thailand, as it does in bos taurus and bos indicus. | 2007 | 17187160 |
| giardia and cryptosporidium in water buffaloes (bubalus bubalis). | a cross-sectional survey of giardia duodenalis and cryptosporidium parvum infection in the water buffalo (bubalus bubalis) was carried out in central italy. a geographical information system (gis) was constructed utilizing as data-layers the topographic base map and the digital aerial photographs of the study area, as well as the geo-referenced points of all the buffalo farms. the survey was conducted on a sample of 90 farms, selected using a grid approach followed by proportional allocation. fo ... | 2007 | 17211662 |
| purification and some properties of galectin-1 derived from water buffalo (bubalus bubalis) brain. | an increasing number of galectins have been found in various animal species, the most abundant of which is galectin-1. the purpose of the present study was to purify and characterize galectin-1 from buffalo brain. we purified the galectin using a combination of ammonium sulphate fractionation and affinity chromatography and the homogeneity was determined by both native polyacrylamide gel electrophoresis (page) and denaturing sds-page. the molecular weight of the galectin as determined by sds-pag ... | 2007 | 17218122 |
| effects of vitrification procedures on subsequent development and ultrastructure of in vitro-matured swamp buffalo (bubalus bubalis) oocytes. | the purpose of the present study was to investigate the effects of two vitrification procedures on developmental capacity and ultrastructural changes of matured swamp buffalo oocytes. in vitro-matured oocytes were vitrified by using 35 and 40% ethylene glycol as vitrification solution for solid surface vitrification (ssv) and in-straw vitrification (isv), respectively. survival rate of vitrified-warmed oocytes, evaluated on the basis of ooplasm homogeneity, oolemma integrity and zona pellucida i ... | 2007 | 17257525 |
| sequence analysis of utr and coding region of kappa-casein gene of indian riverine buffalo (bubalus bubalis). | in this study, complete nucleotide as well as derived amino acid sequence characterization of water buffalo (bubalus bubalis) kappa-casein gene has been presented. kappa-casein cdna clones were identified and isolated from a buffalo lactating mammary gland cdna library. sequence analysis of kappa-casein cdna revealed 850 nucleotides with an open reading frame (orf) of 573 nucleotides, encoding mature peptide of 169 amino acids. the 5' untranslated region (utr) comprised 71 nucleotides, while 3' ... | 2006 | 17076250 |
| dgat1 and abcg2 polymorphism in indian cattle (bos indicus) and buffalo (bubalus bubalis) breeds. | indian cattle (bos indicus) and riverine buffalo (bubalus bubalis) give a poor yield of milk but it has a high fat and protein percentage compared to taurine cattle. the identification of qtls (quantitative trait loci) on bta14 and bta6 and its subsequent fine mapping has led to identification of two non conservative mutations affecting milk production and composition. our objective was to estimate the frequency of k232a (dgat1--diacylglycerol-acyltransferase 1) and y581s (abcg2--atp binding cas ... | 2006 | 17087837 |
| monensin toxicosis in water buffaloes (bubalus bubalis). | the consumption of monensin-containing feed resulted in deaths of water buffaloes from a feedlot in which cattle and buffaloes were kept together. the monensin formulation was recommended only for use in cattle. anorexia, muscular weakness, dyspnea, and recumbency were the major clinical findings. the most significant gross lesions were focal pale areas in semitendinosus and semimembranosus muscles, in which segmental necrosis of myofibers was seen microscopically. to compare susceptibilities of ... | 2006 | 17037623 |
| novel cytogenetic finding: an unusual x;x-translocation in mehsana buffalo (bubalus bubalis). | cytogenetic investigations of a phenotypically normal mehsana river buffalo calf (bubalusbubalis) revealed an xxy chromosome complement due to x;x-translocation in all screened metaphase plates. the chromosomal anomaly was identified by gtg-banding while cbg- and rbg-banding revealed two heterochromatin blocks and that one of the two x chromosomes was late replicating, respectively. the normal cytogenetic profiles of sire, dam and relatives of the calf suggest that the anomaly could have arisen ... | 2006 | 17065802 |
| characterization and kinetics studies of water buffalo (bubalus bubalis) myoglobin. | the colour of buffalo (bubalus bubalis l.) meat is darker than bovine meat. since meat colour depends on the concentration of myoglobin (mb) and its oxidation state, we have determined the main structural and functional properties of buffalo mb. buffalo mb was purified from longissimus dorsi muscles and its molecular mass determined by esi q-tof mass spectrometry. the molecular mass 17,034.50 was 86.20 da higher than the bovine mb. this was confirmed by analysing its primary structure, using a c ... | 2006 | 16959515 |
| cattle rob(1;29) originating from complex chromosome rearrangements as revealed by both banding and fish-mapping techniques. | sixteen carriers of rob(1;29) (one of which was homozygous) from six different breeds (four italian and two portuguese), two heterozygous carriers of rob(26;29), three river buffaloes and two sheep were cytogenetically investigated in this study by using banding and fish-mapping techniques (the latter only in cattle and river buffalo). single- and dual- colour fish were used with bovine probes containing both inra143 (mapping proximally to bta29) and bovine satellite (sat) dna sat i, sat iii and ... | 2006 | 16964571 |
| xylazine, ketamine and their combination for lumbar epidural analgesia in water buffalo calves (bubalus bubalis). | the study was conducted to evaluate the effects of xylazine individually (0.05 mg/kg), ketamine individually (2.5 mg/kg), and a combination of xylazine and ketamine (0.05 mg/kg and 2.5 mg/kg) after lumbar epidural administration in water buffalo calves. fifteen non-descript, male water buffalo calves of 6-8 months of age weighing between 55 and 75 kg were randomly placed in three groups (groups a, b and c). the agents were administered at the first lumbar epidural space. clinico-physiological pa ... | 2006 | 16970633 |
| organizational and expressional uniqueness of a testis-specific mrna transcript of protooncogene c-kit receptor in water buffalo bubalus bubalis. | protooncogene c-kit receptor is implicated with spermatogenesis, melanogenesis, and hematopoeisis, and undergoes tissue/stage specific alternate splicing. we have isolated 2973-bp full-length cdna sequence (cds) of this gene from testis and other tissues of water buffalo bubalus bubalis. upon comparison, the c-kit sequences showed tissue specific nucleotide changes resulting in novel truncated peptides. these peptides lacked intracellular and/or transmembrane domains in all the tissues except te ... | 2006 | 16989573 |
| origin of mitochondrial dna diversity of domestic yaks. | the domestication of plants and animals was extremely important anthropologically. previous studies have revealed a general tendency for populations of livestock species to include deeply divergent maternal lineages, indicating that they were domesticated in multiple, independent events from genetically discrete wild populations. however, in water buffalo, there are suggestions that a similar deep maternal bifurcation may have originated from a single population. these hypotheses have rarely bee ... | 2006 | 16995938 |
| effect of gender on slaughter and carcass traits in anatolian water buffalo. | this study determined the effect of gender on slaughter and carcass traits in anatolian water buffalos reared under free range conditions in turkey. sixteen four-years-old anatolian water buffalo were divided into two groups of eight males in one group and eight females in the other. the slaughter and carcass traits, carcass measurement, amount of worthy meat from each animal and phenotypic correlations among some carcass traits were determined. chilling loss, slaughter weight and hot and cold c ... | 2006 | 17009811 |
| cross-sectional associations between intensity of animal and human infection with schistosoma japonicum in western samar province, philippines. | to estimate the association between the intensity of animal infection with schistosoma japonicum and human infection in western samar province, the philippines. | 2006 | 16799728 |
| physical organization of the 1.709 satellite iv dna family in bovini and tragelaphini tribes of the bovidae: sequence and chromosomal evolution. | repetitive dna in the mammalian genome is a valuable record and marker for evolution, providing information about the order and driving forces related to evolutionary events. the evolutionarily young 1.709 satellite iv dna family is present near the centromeres of many chromosomes in the bovidae. here, we isolated 1.709 satellite dna sequences from five bovidae species belonging to bovini: bos taurus (bta, cattle), bos indicus (bin, zebu), bubalus bubalis (bbu, water buffalo) and tragelaphini tr ... | 2006 | 16825766 |
| pathology of naturally occurring paratuberculosis in water buffaloes (bubalus bubalis). | gross and histologic lesions of paratuberculosis were studied in water buffaloes. small intestines and associated mesenteric lymph nodes of 405 water buffaloes were examined. of these, 20 animals having visible changes of intestinal thickening, mucosal corrugations, and enlargement of mesenteric lymph nodes exhibited histologic alteration characteristics of mild to moderate granulomatous inflammation. the histologic lesions observed in these animals were classified into 3 grades on the basis of ... | 2006 | 16846987 |
| [a comparative study on isoenzymes of sarcocystis spp. from cattle and water buffaloes]. | to analyze the zymogram of peroxidase (per) and phosphoglucose isomerase (pgi) of three species of sarcocystis. | 2006 | 16862907 |
| epigenetic characteristics of cloned and in vitro-fertilized swamp buffalo (bubalus bubalis) embryos. | swamp buffalos are becoming endangered due to reproductive inefficiencies. this is of concern because many countries depend heavily on their products. somatic cell nuclear transfer (scnt) is a potential strategy for preserving endangered species. to date, scnt in swamp buffalo has succeeded in the creation of blastocyst embryos. however, development to term of scnt swamp buffalos is extremely limited, and only 1 live birth has been reported. an abnormal epigenetic mechanism is suspected to be th ... | 2006 | 16864866 |
| comparative assessment of th1 and th2 cytokines of swamp type buffalo and other bubaline breeds by molecular cloning, sequencing and phylogenetics. | comparative assessment of th1 and th2 cytokines of three bubaline breeds namely swamp buffalo, its crossbreed with riverine buffalo (cb), and the improved breed of bulgarian murrah buffalo (bmb), was done by molecular cloning, sequencing and phylogenetic analysis. the th1 cytokines analyzed included il-2, il-12p35, il-12p40, and ifn-gamma while th2 cytokines included il-4 and il-10. both groups showed strict conservation in the putative secondary structures and amino acid residues within the tri ... | 2006 | 16870267 |
| does multiple hosts mean multiple parasites? population genetic structure of schistosoma japonicum between definitive host species. | multi-host parasites, those capable of infecting more than one species of host, are responsible for the majority of all zoonotic, emerging or persistent human and animal diseases and are considered one of the major challenges for the biomedical sciences in the 21st century. we characterized the population structure of the multi-host parasite schistosoma japonicum in relation to its definitive host species by genotyping miracidia collected from humans and domestic animals across five villages aro ... | 2006 | 16876170 |
| interferon-alpha genes from bos and bubalus bubalus. | interferon-a genes were cloned from six breeds of three species of two genera (three chinese native cattle breeds of yellow cattle, wild yak and huanhu domestic yak, one european breed of holstein cow, and two water buffalo breeds of fuan water buffalo and fuzhong water buffalo) by direct pcr. the pcr products were directly inserted into the expression vector to be sequenced and expressed. sequence analysis showed that ifn-a genes of six clones were composed of 498 nucleotides, encoding a mature ... | 2006 | 16621760 |
| chromosomal localization, copy number assessment, and transcriptional status of bamhi repeat fractions in water buffalo bubalus bubalis. | higher eukaryotes contain a wide variety of repetitive dna, although their functions often remain unknown. we describe cloning, chromosomal localization, copy number assessment, and transcriptional status of 1378- and 673-bp repeat fractions in the buffalo genome. the pds5, representing the 1378-bp fragment, showed fish signals in the centromeric region of acrocentric chromosomes only, whereas pds4, corresponding to 673 bp, detected signals in the centromeric regions of all the chromosomes. cros ... | 2006 | 16629593 |
| subcutaneous pharmacokinetics and dosage regimen of cefotaxime in buffalo calves (bubalus bubalis). | the pharmacokinetics and dosage regimen of cefotaxime following its single subcutaneous administration (10 mg/kg) were investigated in buffalo calves. plasma and urine samples were collected over 10 and 24 h post administration, respectively. cefotaxime in plasma and urine was estimated by microbiological assay technique using e. coli as test organism. the pharmacokinetic profiles fitted one-compartment open model. the peak plasma levels of cefotaxime were 6.48 +/- 0.52 microgram/ml at 30 min an ... | 2006 | 16645334 |
| rapid sexing of water buffalo (bubalus bubalis) embryos using loop-mediated isothermal amplification. | loop-mediated isothermal amplification (lamp) is a novel dna amplification method that amplifies a target sequence specifically under isothermal conditions. the objective of this study was to identify a y chromosome-specific sequence in water buffalo and to establish an efficient procedure for embryo sexing by lamp. the homologues of a y chromosome-specific sequence, bovine repeat y-associated.2, in swamp and river buffalo were cloned, and designated swamp buffalo repeat y-associated.2 and river ... | 2006 | 16672158 |
| likelihood ratio estimation without a gold standard: a case study evaluating a brucellosis c-elisa in cattle and water buffalo of trinidad. | the likelihood ratio (lr) is a measure of association that quantifies how many more times likely a particular test result is from an infected animal compared to one that is uninfected. they are ratios of conditional probabilities and cannot be interpreted at the individual animal level without information concerning pretest probabilities. their usefulness is that they can be used to update the prior belief that the individual has the outcome of interest through a modification of bayes' theorem. ... | 2006 | 16600408 |
| cystic echinococcosis in water buffaloes: epidemiological survey and molecular evidence of ovine (g1) and buffalo (g3) strains. | a survey of cystic echinococcosis (ce) in the water buffalo (bubalus bubalis) of the italian mediterranean breed was carried out in campania, a region of southern italy. in addition, a molecular study was performed on 48 hydatid cysts coming from 48 water buffaloes in order to determine the echinococcus granulosus strain(s) present in this host. out of a total of 722 water buffaloes examined for ce, 76 (10.5%) were found infected. the average number of cysts per buffalo was 4.3 (minimum 1, maxim ... | 2006 | 16480832 |
| genetic resistance to brucella abortus in the water buffalo (bubalus bubalis). | brucellosis is a costly disease of water buffaloes (bubalus bubalis). latent infections and prolonged incubation of the pathogen limit the efficacy of programs based on the eradication of infected animals. we exploited genetic selection for disease resistance as an approach to the control of water buffalo brucellosis. we tested 231 water buffalo cows for the presence of anti-brucella abortus antibodies (by the agglutination and complement fixation tests) and the nramp1 genotype (by pcr-denaturin ... | 2006 | 16552040 |
| hematological and biochemical changes in water buffalo calves (bubalus bubalis) infected with trypanosoma evansi. | four water buffalo calves (bubalus bubalis) were each inoculated intravenously with 10(6)t. evansi (camel isolate) and the fifth calf kept as non-infected control. the blood and sera of all calves were examined every 4 days during the first month post-inoculation (pi) and then once weekly until the end of the experiment (88 days pi). they were examined for hematological and biochemical changes, liver and kidney function tests. hemoglobin concentration (hb%), packed cell volume (pcv) and red bloo ... | 2006 | 16567045 |
| cloning and biological characterization of buffalo (bubalus bubalis) interferon-gamma. | interferon-gamma, a major immunomodulatory cytokine, of indian water buffalo (bubalus bubalis) was characterized at molecular level. complementary dna and essential promoter region were cloned and sequenced, and functional recombinant protein was expressed in bacterial system. the cdna has 97.8% nucleotide identity with 11-nucleotide and four-amino acid variations, and the essential promoter region has 98.4% identity with five-nucleotide variations and a four-nucleotide deletion in comparison wi ... | 2006 | 16360017 |
| transcriptional status of known and novel genes tagged with consensus of 33.15 repeat loci employing minisatellite-associated sequence amplification (masa) and real-time pcr in water buffalo, bubalus bubalis. | we conducted minisatellite-associated sequence amplification (masa) with an oligo (5' cacctctccacctgcc 3') based on consensus of 33.15 repeat loci using cdna from the testis, ovary, spleen, kidney, heart, liver, and lung of water buffalo bubalus bubalis and uncovered 25 amplicons of six different sizes (1,263, 846/847, 602, 576, 487, and 324 base pairs). these fragments, cloned and sequenced, were found to represent several functional, regulatory, and structural genes. blast search of all the 25 ... | 2006 | 16405399 |
| identification of x- and y-chromosome bearing buffalo (bubalus bubalis) sperm. | the aim of this study was to identify the difference in dna content characterizing the x- and y-chromosome bearing sperm of buffalo. sperm from six murrah buffaloes and six nili-ravi buffaloes were collected and stained with hoechst 33342 followed by flow cytometry analysis of the dna content. two symmetrical, separate but overlapping peaks presumed to be x- and y-chromosome bearing sperm were detected. the difference in fluorescence intensity, which related to the dna content, between the x- an ... | 2006 | 16413705 |
| increased frequencies of both chromosome abnormalities and sces in two sheep flocks exposed to high dioxin levels during pasturage. | during the past four years several livestock farms (sheep, cattle and river buffalo) in the provinces of naples and caserta (southern italy) have been unable to sell their milk and other dairy products due to the levels of dioxins (17 different types) present in the milk mass exceeding the value permitted [3 pg/g of fat, as human who 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd or dioxin) equivalent]. while some farms, especially those showing relatively low levels of dioxins, have managed to reduc ... | 2006 | 16434450 |
| buffalo (bubalus bubalis) interleukin-12: analysis of expression profiles and functional cross-reactivity with bovine system. | interleukin-12, a heterodimeric pro-inflammatory cytokine, from water buffaloes (bubalus bubalis) was analyzed for its for its tissue specific expression and functionality. concanavalin a stimulated splenocytes displayed an up-regulation of the il-12 p40 subunit 8-24h post-stimulation, whereas the p35 subunit did not show any quantitative variation at different time intervals. basal level expressions of both the subunits were observed by rt-pcr in spleen. in addition p40 transcripts could be det ... | 2006 | 16112195 |
| serologic responses, biosafety and clearance of four dosages of brucella abortus strain rb51 in 6-10 months old water buffalo (bubalus bubalis). | thirty water buffalo were obtained from a brucellosis-free farm in order to evaluate antibody responses, bacterial clearance and safety to brucella abortus strain rb51 vaccine in a dose response study. the animals were randomly divided into five treatment groups. groups i-v received the recommended dose of rb51 vaccine (rd) once, rd twice 4 weeks apart, double rd once, double rd twice 4 weeks apart and saline once, respectively. antibody responses to rb51 were monitored at 2, 4, 6, 8, 10, 12, 16 ... | 2006 | 16112746 |
| phylogeny of trypanosoma ( megatrypanum ) theileri and related trypanosomes reveals lineages of isolates associated with artiodactyl hosts diverging on ssu and its ribosomal sequences. | ssu ribosomal sequences of trypanosomes from brazilian cattle and water buffalo were used to infer phylogenetic relationships between non-pathogenic t. theileri and allied species parasitic in artiodactyls. t. theileri trypanosomes from distinct geographical regions in brazil and from other countries were tightly clustered into the 'clade t. theileri' distant from the 't. brucei clade' of pathogenic parasites of artiodactyls, and also distinct from trypanosomes of other mammals. the existence of ... | 2006 | 16197590 |
| molecular characterization and prokaryotic expression of buffalo (bubalus bubalis) interleukin-6. | the study describes the characterization of interleukin-6 cdna and essential promoter sequences of the indian water buffalo (bubalus bubalis) and expression of the recombinant il-6 in escherichia coli. buffalo il-6 shows very high nucleotide level identity of the cdna (98.7%) and promoter (98%) sequences with the corresponding cattle sequences. all the major regulatory elements of il-6 promoter like ap-1, multiple response element, nf-il6, ets binding domain and nf-kappab binding sites show abso ... | 2006 | 16199257 |
| changes in uterine protein secretion during luteal and follicular phases and detection of phosphatases during luteal phase of estrous cycle in buffaloes (bubalus bubalis). | changes in uterine proteins during different reproductive states and their functional significance though known in other species have not been established in buffaloes. an attempt has been made to unravel the changes in composition of buffalo uterine secretion with growth and regression of corpora-lutea during early, mid and late luteal and follicular phase of estrous cycle using gel filtration and electrophoresis techniques. also the phosphatases activities in luteal phase uterine secretions ha ... | 2006 | 16213013 |
| binucleate trophoblast giant cells in the water buffalo (bubalus bubalis) placenta. | the binucleate trophoblast giant cells (bnc) of the water buffalo, bubalus bubalis, placenta were studied, with emphasis on the synthesis of bnc-specific proteins. placentomal tissues of 27 water buffalos (2-10 months of pregnancy) were processed for light and electron microscopy. the frequency of bncs was 20% of the trophoblastic cells in 2-3-month placentas and increased to 27% in the later stages. ultrastructurally, binucleate cells displayed a prominent granular endoplasmic reticulum and gol ... | 2006 | 16240388 |
| ear-tag retention and identification methods for extensively managed water buffalo (bubalus bubalis) in trinidad. | thirty-two young domestic water buffalo were studied to evaluate ear-tag retention during an epidemiologic field trial. plastic ear-tags were placed in both ears before the start of the trial, which was implemented in an extensively managed domestic water buffalo herd of approximately 1000 animals in trinidad from 1999-2001. the presence or absence of ear-tags was recorded at the times of animal handling. the rate of ear-tag loss was modeled using a parametric survival analysis assuming an expon ... | 2006 | 16242197 |
| anomalous mrna levels of chromatin remodeling genes in swamp buffalo (bubalus bubalis) cloned embryos. | the swamp buffalo (bubalus bubalis) is a multi-purpose animal in agriculture that is challenged by extinction due to low reproductive efficiency. nuclear transfer (nt) has been used to preserve special breeds of buffalo, as well as to increase the number of animals. however, cloned buffalo embryos have impaired development, as in other species. to understand the chromatin remodeling activities in cloned embryos and to improve nt technology, we examined the expression profiles of five genes invol ... | 2006 | 16256185 |
| comparative fish mapping of mucin 1, transmembrane (muc1) among cattle, river buffalo, sheep and goat chromosomes: comparison between bovine chromosome 3 and human chromosome 1. | four bovine bac clones (0494f01, 0069d07, 0060b06, and 0306a12) containing muc1, as confirmed by mapping muc1 on a rh3000 radiation hybrid panel, were hybridised on r-banded chromosomes of cattle (bta), river buffalo (bbu), sheep (oar) and goat (chi). muc1 was fish-mapped on bta3q13, bbu6q13, oar1p13 and chi3q13 and both chromosomes and chromosome bands were homoeologous confirming the high degree of chromosome homoeologies among bovids and adding more information on the pericentromeric regions ... | 2006 | 16276097 |
| histo-morphology of the uterus and early placenta of the african buffalo (syncerus caffer) and comparative placentome morphology of the african buffalo and cattle (bos taurus). | differences exist in reproductive physiology between african buffalo (syncerus caffer), cattle (bos taurus) and water buffalo (bubalus bubalis). the aim of this study was to histo-morphologically compare the anatomy of non-pregnant and pregnant uteri of buffalo and cattle. two non-pregnant uteri and placentae of six pregnant african buffalo were used. early placentome formation (fetal crown rump length (crl): 2-17.5 cm) in s. caffer and b. taurus was compared. the endometrium of buffalo uteri co ... | 2006 | 16293303 |
| nucleic acids and protein content in relation to growth and regression of buffalo (bubalus bubalis) corpora lutea. | information on nucleic acids and protein content of buffalo corpus luteum (cl) in relation to growth, development and regression is not available. an experiment was thus conducted to investigate the variation and relationship between nucleic acids and protein content in cl during different developmental stages and to determine the qualitative differences in protein constituents in any of these stages. buffalo corpora lutea of different developmental stages viz., developing (day 5-10, n = 16), de ... | 2006 | 16171957 |
| development of water buffalo (bubalus bubalis) embryos from in vitro matured oocytes reconstructed with fetal skin fibroblast cells as donor nuclei. | the present study was carried out to explore the feasibility of using buffalo fetal skin fibroblasts as donor nuclei and to find out the developmental competence of embryos following transfer of these nuclei to in vitro matured enucleated buffalo oocytes. skin cells were isolated from 1 to 2-month-old fetuses obtained from slaughterhouse, by enzymatic digestion (0.5% w/v trypsin +0.05% w/v collagenase in dulbecco's pbs) for 15-20 min. the cells were washed 4 times with dulbecco's pbs and then on ... | 2006 | 16182475 |
| the role of wild animal populations in the epidemiology of tuberculosis in domestic animals: how to assess the risk. | tuberculosis is present in wild animal populations in north america, europe, africa and new zealand. some wild animal populations are a source of infection for domestic livestock and humans. an understanding of the potential of each wild animal population as a reservoir of infection for domestic animals is reached by determining the nature of the disease in each wild animal species, the routes of infection for domestic species and the risk of domestic animals encountering an infectious dose. the ... | 2006 | 16326039 |