Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| brucella mycotic aneurysm of ascending aorta complicating discrete subaortic stenosis. | 1993 | 8498327 | |
| isolation of brucella melitensis from human milk. | 1993 | 8505580 | |
| [immunobiological characteristics of brucella outer membrane proteins, genetic determinants of which are expressed in escherichia coli cells]. | the influence of the brucella omp preparations obtained by the genetic engineering technique (18, 38 and 18 + 38 kd) on the formation of the specific protection and progress of infectious process in brucellosis in the in vivo experiments has been studied. the omps synthesized in escherichia coli cells gse579 and having mol mass 18 and 38 kd are common antigens for a number of brucella species. the 18 kd omp was found to protect 66.7% of experimental animals against brucellosis, while the protect ... | 1993 | 8510678 |
| hypothermia as a presenting sign of brucellosis. | 1993 | 8513071 | |
| [the effect of immunomodulators on the development of the infectious and vaccinal processes as well as on the efficacy of therapeutic preparations in brucellosis]. | 1993 | 8079591 | |
| efficacy of brucella suis strain 2 vaccine against brucella ovis in rams. | the protective efficacy against brucella ovis of live vaccine brucella suis strain 2 (s2) and brucella melitensis strain rev 1 has been evaluated in rams. fourteen 4-month-old brucella-free aragonesa rams were vaccinated conjunctivally with 2 x 10(9) c.f.u. s2. sixteen rams of the same breed, condition and age were conjunctivally vaccinated the same day with 1.6 x 10(9) rev 1. thirteen rams were unvaccinated controls. eight months after vaccination all rams were challenged with 6 x 10(9) c.f.u. ... | 1993 | 8296481 |
| [acute brucellosis, pancytopenia and bone marrow hypoplasia]. | 1993 | 8289516 | |
| brucellosis: differential diagnosis of acute abdominal pain. | a 34-year-old turkish woman presented with septic fever, sweats, arthralgia, and abdominal pain. further examination revealed generalized lymphadenopathy, hepatosplenomegaly, pancytopenia, and multiple caseous granulomas. microbiological diagnosis revealed brucella melitensis type 3 as causative agent. this case report demonstrates that abdominal pain can be a symptom in brucellosis, and caseous granulomas may occur. in our mobile society brucella infection should be considered as possible diffe ... | 1993 | 8291280 |
| [osteoarthritis caused by brucella melitensis: isolation of the microorganism from the synovial fluid]. | brucellosis is an unusual disease in childhood but the organ manifestations are even more unusual. the authors describe an osteoarthritis caused by brucella melitensis, localized at the tarsal scaphoid in a three years old calabrian child. | 1993 | 8197028 |
| brucellosis presenting as prolonged fever and hemophagocytosis. | 1993 | 8213671 | |
| successful therapeutic regimens for treating brucella melitensis and brucella abortus infections in cows. | three therapeutic regimens were evaluated in 121 cows naturally infected with brucella melitensis or brucella abortus, using a combination of long-acting oxytetracycline (la-otc), streptomycin (st) and otc-intramammary infusion (imi). cessation of shedding of brucella in udder secretions and absence of brucella in selected tissues were considered criteria for successful treatment. regimen a (tested on 35 cows) consisted of la-otc 25 mg/kg administered intramuscularly (i.m.) every 3 days for 42 d ... | 1993 | 8219341 |
| anti-brucella activity of ro 23-9424, a dual-action antibacterial. | the in vitro activity of dual-acting antibacterial ro 23-9424 was determined by the agar dilution method against 126 clinical isolates of brucella melitensis. it was compared with fleroxacin, ciprofloxacin and five conventional drugs. mic50 and mic90 for ro 23-9424 were 1.0 and 4.0 mg/l, respectively, as compared with 0.25 and 0.5 mg/l for fleroxacin and ciprofloxacin. one strain which was resistant to other fluoroquinolones, with an mic of > 8.0 mg/l, did not show cross-resistance to ro 23-9424 ... | 1993 | 8222864 |
| brucella peritonitis. | we report the case of a patient with peritonitis caused by brucella melitensis who also had chronic liver disease. at first the patient was treated for bacterial peritonitis but when a lymphocytic ascites was aspirated antituberculosis chemotherapy was given. however, the serological tests for brucellosis were strongly positive and subsequently b. melitensis was isolated from ascitic fluid. | 1993 | 8228298 |
| prevalence of camel brucellosis in libya. | sera of 967 camels of both sexes were tested for antibodies to brucella using the rose bengal plate test, serum agglutination test and the complement fixation test. the prevalence of positive sera was 4.1 per cent. samples collected for cultural examination revealed 9 isolates. five isolates were from milk samples, 3 from aborted foetuses and one from a vaginal swab. all isolates were identified and biotyped as brucella melitensis biovar 1. | 1993 | 8236486 |
| evaluation of whole cell and subcellular vaccines against brucella ovis in rams. | five antigen preparations from brucella ovis strain reo 198 were incorporated with the pluronic polymer l-121 and muramyl dipeptide and tested as vaccines against b. ovis infection of rams. the antigenic preparations were: (1) a fraction enriched in outer membrane proteins and rough lipopolysaccharide (hot saline extract, hs); (2) the proteins from hs substantially free of lipopolysaccharide; (3) outer membrane blebs; (4) outer membrane-peptidoglycan complexes extracted with detergent; (5) kille ... | 1993 | 8236802 |
| quinolones in treatment of human brucellosis: comparative trial of ofloxacin-rifampin versus doxycycline-rifampin. | quinolones have been reported to be active against brucella species in vitro. in this prospective randomized study, the efficacy and safety of the combination of ofloxacin plus rifampin were compared with the efficacy and safety of doxycycline plus rifampin, both combinations administered for a 6-week period in treatment of brucellosis. sixty-one patients were enrolled in the study, and 49 had blood or bone marrow cultures positive for brucella melitensis. thirty patients received 200 mg of doxy ... | 1993 | 8239591 |
| simultaneous expression of smooth and rough phase properties related to lipopolysaccharide in a strain of brucella melitensis. | brucella strains exhibit either a rough (r) or a smooth (s) colonial phase identifiable by bacteriological methods. this depends on the biosynthesis and translocation to the surface in s but not in r strains, of the o-polysaccharide chain of the lipopolysaccharide (lps) molecule. b. melitensis biovar 1 strain ep exhibited simultaneously both s and r characteristics in relation to colonial morphology, agglutination by monospecific anti-m and anti-r sera, activity of bacteriophages lytic for rough ... | 1993 | 8246253 |
| brucellosis of the spine. | the spinal form of brucellosis is still a disabling disease in many countries outside north america and northern and central europe. fifteen consecutive cases of spinal brucellosis diagnosed and treated over a 20-year period were reviewed retrospectively. six patients were farmers, while 10 patients had a history of ingestion of unpasteurized milk or other dairy products. a high index of suspicion is necessary for the diagnosis, since there are no pathognomonic signs or symptoms. radiological as ... | 1993 | 8264880 |
| [brucellosis: case report and synopsis of 10 cases (1973-1992) in the chur canton hospital]. | a 33-year-old patient from former yugoslavia presented with low back pain and fever. plain x-rays and myelography were normal, but a technetium-99m scan was suspect for vertebral osteomyelitis. serology and blood cultures were positive for brucella (melitensis). antibiotic therapy with tetracycline and rifampicine rapidly improved the signs and symptoms of the infection. a retrospective analysis of 11 cases of brucellosis treated in the kantonsspital, chur, over the years 1973 to 1992 revealed t ... | 1993 | 8272813 |
| therapy of experimental murine brucellosis with streptomycin alone and in combination with ciprofloxacin, doxycycline, and rifampin. | the in vivo efficacy of streptomycin (str), doxycycline (dox), rifampin (rif), ciprofloxacin (cip), and their combinations was evaluated for a brucella melitensis experimental infection in a mouse model. animals were infected with 2 x 10(4) to 4 x 10(4) cfu of b. melitensis intraperitoneally on day 0 and were randomized to receive, starting on day 7, str alone at 75, 150, or 300 mg/kg of body weight per day intraperitoneally or dox at 6 mg/kg/day orally, rif at 3 mg/kg/day orally, or cip at 200 ... | 1993 | 8285615 |
| sensorineural hearing loss in neurobrucellosis. | a case of neurobrucellosis presenting to the otologist with sensorineural hearing loss (snhl) as the predominant clinical feature is reported. the diagnostic features and treatment options are discussed and the need for prolonged combination treatment to prevent relapse and further deterioration of hearing stressed. snhl in neurobrucellosis has hitherto been reported principally in neurology literature as something of an incidental finding and so escapes the attention of otologists. it is hoped ... | 1993 | 8288975 |
| identification and sequence analysis of is6501, an insertion sequence in brucella spp.: relationship between genomic structure and the number of is6501 copies. | an insertion sequence (is) element of brucella ovis, named is6501, was isolated and its complete nucleotide sequence determined. is6501 is 836 bp in length and occurs 20-35 times in the b. ovis genome and 5-15 times in other brucella species. analysis of the junctions at the sites of insertion revealed a small target site duplication of four bases and inverted repeats of 17 bp with one mismatch. is6501 presents significant similarity (53.4%) with is427 identified in agrobacterium tumefaciens, su ... | 1993 | 8126444 |
| [the effect of the in-vitro interaction of antibacterial preparations on brucella melitensis]. | the character of drug interaction in 18 various combinations of sisomicin, amikacin, gentamicin, cefotaxime, doxycycline, trimethoprim and nitroxaline was studied by the chess board method with respect to 25 strains of brucella melitensis. the synergistic effect with respect to the majority of the cultures was observed with the use of the combinations of gentamicin, amikacin or sisomicin with doxycycline. the combinations of the aminoglycosides with cefotaxime and sisomicin or gentamicin with ri ... | 1993 | 8085903 |
| brucella melitensis rev-1 vaccine as a cause of human brucellosis. | 1993 | 8103891 | |
| unusual suppurative complications of brucellosis in children. | three cases with suppurative complications of brucella melitensis infection are presented, demonstrating localized involvement of the brain, bones and lungs, respectively, and the lesions were well defined on radiographic examination. the diagnosis was made on the basis of significant brucella titres and positive blood culture. awareness of such complications and performance of the appropriate serological and bacteriological studies will establish the diagnosis and will also differentiate this d ... | 1993 | 8111184 |
| brucella melitensis growth on loewenstein-jensen egg medium from a case of brucella meningitis. | bacteriologic investigation of a cerebrospinal fluid (csf) specimen for mycobacteria on lowenstein-jensen egg medium revealed the presence of brucella organisms. this coincided with a significant antibody titer against brucella spp. this is the first documented report of the ability of b. melitensis organisms to grow on loewenstein-jensen egg medium. | 1993 | 8112041 |
| monoclonal antibodies to brucella rough lipopolysaccharide: characterization and evaluation of their protective effect against b. abortus. | we characterized 4 monoclonal antibodies (mab) specific for rough lipopolysaccharide (r-lps) of brucella. mab were selected by enzyme-linked immunosorbent assay (elisa) on whole b. abortus 45/20 rough cells and r-lps from b. melitensis b115 rough cells. specificity was confirmed by immunoblot analysis using r-lps and smooth lps (s-lps) preparations. anti-r-lps revealed the low molecular mass r-lps molecules below 20.1 kda in the r-lps and s-lps preparations as well as the typical a and m pattern ... | 1993 | 7514802 |
| brucella antigens--old dogmas, new concepts. | a brief review is given on concepts regarding brucella antigens, principally the lipopolysaccharide (lps) and o-polysaccharide but also, to a minor extent, proteins and lipids. although these views were reported years ago in the literature and became widely accepted at the time, many have since been disproven with results that have led to exciting new avenues of research and application. where gaps, contradictions and anecdotal observations suggest that the current understanding of brucella is l ... | 1993 | 7519357 |
| characterization of o-polysaccharide specific monoclonal antibodies derived from mice infected with the rough brucella melitensis strain b115. | twenty-two monoclonal antibodies (mabs) specific for smooth lipopolysaccharide (s-lps) were generated by fusion of spleen cells from mice infected with the rough brucella melitensis strain b115 with the nso myeloma. according to reactivity in enzyme-linked immunosorbent assay (elisa) with o-polysaccharide (o-ps) and absence of reactivity with rough lipopolysaccharide (r-lps), it was postulated that these mabs recognized epitopes present on the o-ps. most of the mabs reacted equally well in elisa ... | 1993 | 7690392 |
| [the clinico-epidemiological characteristics of brucellosis in young people]. | the article describes an outbreak of brucellosis among the servicemen (age 19-32) which was caused by br. melitensis. 13% of servicemen in the military unit went through this disease. this outbreak was caused by unsatisfactorily treated mutton of sheep which were affected with brucellosis. the illness had acute septic course. the appearance of the focuses of disease in locomotor system took place only when the infectious process had a rather long period (septicometastatic course). the acute sept ... | 1992 | 1292191 |
| [a carboxylated latex agglutination test for the serological diagnosis of human and animal brucellosis]. | a carboxylated latex agglutination test was developed for detecting antibodies in sera of human and animals with brucellosis. the latex was chemico-linked with b. melitensis 16m extracted with 5% nacl. comparing with physico-sorbent latex antigens in detecting antibodies in sera of human and animals with brucellosis, the chemico-linked latex is more stable, more specific and more sensitive. in addition, preparation of chemico-linked latex and some factors affecting the result of the test were al ... | 1992 | 1300250 |
| control of brucellosis in kuwait by vaccination of cattle, sheep and goats with brucella abortus strain 19 or brucella melitensis strain rev. 1. | in kuwait, approximately 12,000 dairy cows were vaccinated with a reduced dose of 3 x 10(9) brucella abortus strain 19 and approximately 350,000 sexually mature sheep and goats with a reduced dose of 10(7) b. melitensis strain rev. 1. using the criteria of prevaccinal and postvaccinal incidences of antibodies, abortions, and human cases of brucellosis, the programme was very successful. widespread vaccination of adult animals is the most effective method of controlling brucellosis among cattle, ... | 1992 | 1306918 |
| brucella melitensis splenic abscess: sonographic detection and follow-up. | 1992 | 1316379 | |
| in vitro activity of azithromycin and tetracycline against 358 clinical isolates of brucella melitensis. | the in vitro activity of azithromycin against human pathogenic strains of brucella melitensis was tested at three centres and compared to that of tetracycline, the standard antibiotic currently used for the treatment of human brucellosis. mic determination was carried out. tested concentration ranges for both azithromycin and tetracycline were between 0.03 and 16 micrograms/ml. brucella melitensis biotype 1, strain m16 was employed as a control microorganism. a total of 358 brucella melitensis s ... | 1992 | 1317801 |
| correspondence analysis in medical research. | various applications of correspondence analysis to biomedical data are presented. the basic concepts of profile, mass and chi-squared distance are introduced in an initial simple example using data on the relationship between headache types and age. the main result of the correspondence analysis is a geometric map of this relationship showing how the relative frequencies of headache types change with age. a second example maps the association between personality types and various medical diagnos ... | 1992 | 1341654 |
| biochemical mis-identification of brucella melitensis and subsequent laboratory-acquired infections. | brucella species are mis-identified in the api 20ne system as moraxella phenylpyruvica (profile number 1200004). since some brucella spp. grow readily in routine blood culture medium and may be isolated from patients without clinically obvious brucellosis, the risk of laboratory-acquired brucellosis exists. we describe two such cases. | 1992 | 1358958 |
| protection against brucella melitensis or brucella abortus in mice with immunoglobulin g (igg), iga, and igm monoclonal antibodies specific for a common epitope shared by the brucella a and m smooth lipopolysaccharides. | mice passively immunized prior to a challenge infection with immunoglobulin g (igg) and igm monoclonal antibodies (mabs) specific for a common epitope of both a- and m-dominant strains had viable brucella abortus 544 or brucella melitensis h38 counts in the spleen reduced to the same extent as did mice passively immunized with mabs specific for either the a or the m epitope. the iga mab was not effective. | 1992 | 1370276 |
| o-chain expression in the rough brucella melitensis strain b115: induction of o-polysaccharide-specific monoclonal antibodies and intracellular localization demonstrated by immunoelectron microscopy. | spleen cells from mice infected with the rough brucella melitensis strain b115 were fused with nso myeloma cells. hybridoma supernatants were screened in elisa with cell walls (cw), sonicated cell extracts (ce) and rough lipopolysaccharide (r-lps) of b. melitensis strain b115 and whole b. melitensis b115 cells. surprisingly, 22 monoclonal antibodies (mabs) reacting in elisa with both cw and ce but not with r-lps and bacterial cells were shown by immunoblot analysis and elisa to react with smooth ... | 1992 | 1382111 |
| purification, characterization, and seroactivity of a 20-kilodalton brucella protein antigen. | an internal protein was purified from cell extracts of brucella melitensis b115 by a combination of preparative isoelectric focusing and high-performance size exclusion chromatography. the protein has an apparent molecular mass of 230 kda as determined by size exclusion chromatography. the protein was resolved to a single band of 20 kda after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the native protein had an isoelectric point of 4.9. the n-terminal sequence of the 20-kda protei ... | 1992 | 1400966 |
| [osteoarticular manifestations of brucellosis. apropos of 2 cases of brucellosis-induced sacroiliitis]. | 1992 | 1411027 | |
| [elaboration of a rapid method of determining the sensitivity of brucella to antibiotics]. | optimal conditions were developed for determination of antibiotic sensitivity in brucella by using enzyme immunoassay directly in the primary cultures of the material tested. the brucella concentration in the material tested should be not lower than 1.10(6) microbial cells/ml and the time of culture incubation be 24 hours at 37 degrees c. the obligatory condition is to use a liquid medium, i.e. the albimi broth with 1% glucose. to inhibit the foreign microflora it is recommended to use polymyxin ... | 1992 | 1417320 |
| a rapid and sensitive method for the identification of brucella species with a monoclonal antibody. | a coagglutination test using monoclonal antibody bme10-5 with specificity for the m antigen of brucella melitensis 16m has been developed for the rapid identification of the smooth brucella species. all reference strains of several biovars of b. melitensis, b. abortus, b. suis and b. neotomae tested were positive in this assay. no significant differences in reaction intensity were observed in relation to the different distribution of the a and m antigens among the brucella serovars analysed. con ... | 1992 | 1448627 |
| antibody response to brucella outer membrane proteins in bovine brucellosis: immunoblot analysis and competitive enzyme-linked immunosorbent assay using monoclonal antibodies. | sera from brucella-infected bovines were analyzed by immunoblotting by using sonicated cell extracts of b. melitensis or b. abortus and a competitive enzyme-linked immunosorbent assay (elisa) with monoclonal antibodies against outer membrane proteins (omps) with molecular masses of 10, 16.5, 19, 25 to 27, 36 to 38, and 89 kda. antibody responses against omps were compared with antibody responses against smooth lipopolysaccharide. immunoblot analysis indicated that the antibody response in infect ... | 1992 | 1452700 |
| isolation of brucella melitensis from aborting ruminants in jordan. | thirty-four isolates (16.5 per cent) of brucella melitensis were cultured from 206 samples from aborted, still-born or weak full-term animals and vaginal swabs from aborted animals. twenty-six of 31 isolates from sheep were b. melitensis biovar 3. two isolates were biovar 1 and one of these was the vaccine strain rev 1. the three remaining isolates were from vaginal swabs and were not biotyped since b. melitensis biovar 3 had been isolated from the aborted fetuses. one of two isolates from cattl ... | 1992 | 1452817 |
| brucellosis with nephrotic syndrome, nephritis and iga nephropathy. | a patient with systemic brucellosis due to brucella melitensis had severe renal involvement. clinical features included hypertension, macroscopic haematuria, massive proteinuria of 10 g per 24 hours and azotaemia. following treatment with antibiotics, the azotaemia resolved and proteinuria decreased to less than 0.5 g per 24 hours, but microscopic haematuria and hypertension persisted. renal biopsy during recovery revealed iga nephropathy with minimal mesangial changes, suggesting a causal relat ... | 1992 | 1461859 |
| comparative in vitro activity of clarithromycin. spanish collaborative group. | the activity in vitro of clarithromycin, a new macrolide, was compared to that of various antibiotics in tests using 3,880 clinical isolates. clarithromycin was two times more active than erythromycin against staphylococcus aureus, enterococcus faecalis, enterococcus faecium, streptococci of groups c, g and f, brucella melitensis, legionella pneumophila and mycoplasma spp., 16 times more active against ureaplasma urealyticum and 2 to 4 times less active against campylobacter spp. in general, cla ... | 1992 | 1468430 |
| the ability of brucella melitensis to grow on loewenstein-jensen egg medium: presentation of a case with brucella meningitis. | 1992 | 1468897 | |
| serological and bacteriological study of brucellosis in camels in central saudi arabia. | sera from 2,630 apparently normal adult camels (camelus dromedarius) raised in central saudi arabia (riyadh and al-kharj cities) were examined serologically by the rose bengal and standard united states of america brucella plate agglutination tests. the overall seroprevalence of brucellosis in the restricted populations of tested camels was 8%. the seroprevalence of brucellosis among camels raised in small numbers in the backyards of 24 houses in riyadh and those intensively raised on one large ... | 1992 | 1472730 |
| treatment of brucella melitensis infection in sheep and goats with oxytetracycline combined with streptomycin. | six treatment regimens using oxytetracycline (otc) combined with streptomycin (st) were evaluated for eliminating brucella melitensis from 480 naturally-infected sheep and goats. cessation of shedding brucella from udder secretions and absence of brucella in selected tissues at autopsy were considered criteria for successful treatment. four regimens were equally effective in eliminating brucella in the treated groups of sheep and goats regardless of the source of antibiotics used. these were reg ... | 1992 | 1472731 |
| [identification and characterisation of 200 strains of brucella canis under test from china]. | 200 cultures of brucella canis under test were received from some provinces in our laboratory. 167 (85.59%) of them were identified as brucella according to morphology, growth properties and serologies. and then, they were proved as brucella canis with r-serum agglutination and lysed test of phage r/c. some strains also were performed dna homologous determination and observation of electron microscopic morphology. the results found out that the genetic constitution and picture of them identified ... | 1992 | 1481533 |
| [the spectrum of pathogens in positive blood cultures--tyrol 1991]. | in a recent study by the centers for disease control (cdc) it was noted that there had been a resurgence of gram-positive bacteremia together with an increase in fungemia. this reported trend is confirmed by data from the austrian tirol. in 1991 1,750 out of 13,679 specimens (12.8%) yielded bacterial or fungal growth, accounting for 1,248 cases of "bacteremia"; no decision was made about the clinical significance of the culture isolates. we consider laboratory reports of blood isolates to be fai ... | 1992 | 1481545 |
| unrecognised neurobrucellosis giving rise to brucella melitensis peritonitis via a ventriculoperitoneal shunt. | 1992 | 1486897 | |
| [endocarditis caused by brucella melitensis on a mitral valve prosthesis presenting as an ischemic cerebrovascular accident]. | a new case of endocarditis by brucella melitensis on a mitral valve prosthesis in a 15 year old patient, whose first manifestation was an ischemic cerebrovascular accident is reported. the patient presented with daily fever only two months later. medical treatment alone was not sufficient to avoid valvular failure and substitution of the prosthesis was required. the clinical manifestations and complications of this infrequent condition is discussed. treatment, which often requires the combinatio ... | 1992 | 1489778 |
| detection of brucella melitensis and brucella abortus by dna amplification. | suitable reaction conditions and oligonucleotide primers were sought for the detection of brucella melitensis and brucella abortus by the polymerase chain reaction. primers were chosen from within the coding sequence of a gene encoding a 31 kda b. abortus antigen. the test was shown to be sensitive, and specificity was demonstrated using dna derived from a panel of gram-negative pathogens. there was no detectable difference between b. melitensis and b. abortus in the sensitivity of the reaction ... | 1992 | 1495123 |
| [evolution of antibiotic sensitivity in brucella melitensis]. | 1992 | 1498179 | |
| therapy of experimental murine brucellosis with streptomycin, co-trimoxazole, ciprofloxacin, ofloxacin, pefloxacin, doxycycline, and rifampin. | mice infected with brucella melitensis were treated with streptomycin, co-trimoxazole, ciprofloxacin, doxycycline, and rifampin intraperitoneally and with ciprofloxacin, ofloxacin, pefloxacin, doxycycline, and rifampin orally for 14 to 21 days. doxycycline- and rifampin-treated animals (either route) demonstrated a cure rate significantly better than that of controls. longer therapy periods were associated with a significantly better outcome. therapy failure was observed in all mice treated with ... | 1992 | 1510422 |
| an elisa with brucella lipopolysaccharide antigen for the diagnosis of b. melitensis infection in sheep and for the evaluation of serological responses following subcutaneous or conjunctival b. melitensis strain rev 1 vaccination. | an indirect enzyme-linked immunosorbent assay (elisa) with unpurified brucella melitensis smooth lipopolysaccharide (s-lps) as antigen was evaluated for the serological diagnosis of b. melitensis infection in sheep in comparison with the rose bengal (rb), complement fixation (cf), radial immunodiffusion (rid), microplate agglutination (ma) and rivanol agglutination (riv) tests. tests rb and cf detected as positive each of the 77 sera from b. melitensis-infected animals tested, the rid (74), ma ( ... | 1992 | 1557898 |
| preliminary studies on the use of carbon substrate utilization patterns for identification of brucella species. | a commercial system (biolog, hayward, ca) that uses reduction of an indicator dye to determine the oxidation of 95 different carbon substrates contained in a defined minimal medium was tested with 35 isolates of brucella spp. to determine if the system could be used in place of respirometric methods to identify species. of 95 substrates contained in this system, three were oxidized by all the brucella strains tested, 48 were oxidized by none of the strains tested, and 44 were oxidized differenti ... | 1992 | 1572134 |
| brucellosis induced avascular necrosis of the femoral head in a 7 year old child. | the case is reported of a 7 year old bedouin girl who presented with a three week history of fever and limp. initial tests for rheumatoid factor, antinuclear antibodies, and brucella were negative. two weeks after admission brucella melitensis was cultured from bone marrow and synovial fluid samples. the patient improved after treatment with doxycillin and streptomycin, but radiographs of the right hip showed avascular necrosis of the right femoral head. a mild limp and limitation of motion in t ... | 1992 | 1575594 |
| efficacy of ciprofloxacin for treatment of brucella melitensis infections. | the effectiveness of treatment of human brucellosis caused by brucella melitensis with ciprofloxacin alone was investigated in a prospective nonrandomized study. subjects with central nervous system involvement, endocarditis, or severe renal dysfunction; children under 16 years of age; and pregnant women were excluded from the study. of 19 patients, 16 completed the study; 7 were diagnosed as having acute systemic brucellosis, and 9 had acute brucella arthritis-diskitis. a rapid response to cipr ... | 1992 | 1590681 |
| evaluation of polysaccharide, lipopolysaccharide, and beta-glucan antigens in gel immunodiffusion tests for brucellosis in cattle. | in venezuela, 1,012 cattle sera were screened for their ability to precipitate brucella melitensis 16m smooth-lipopolysaccharide (s-lps), b melitensis b115 polysaccharide b (poly b), b abortus 1119-3 o-polysaccharide (ps), or b abortus 1119-3 cyclic 1,2 linked beta-d-glucan (beta-glucan) in an agar-gel immunodiffusion assay. these sera were previously classified as being brucella abortus-infected, s-19-vaccinated, or negative after an assessment of historical records and results of 5 standard se ... | 1992 | 1595966 |
| treatment of human brucellosis with doxycycline plus rifampin or doxycycline plus streptomycin. a randomized, double-blind study. | to compare the effectiveness of doxycycline-rifampin (dr) combination therapy with that of the classic doxycycline-streptomycin (ds) combination in patients with brucellosis. | 1992 | 1596044 |
| conjunctival vaccination of pregnant ewes and goats with brucella melitensis rev 1 vaccine: safety and serological responses. | when brucella melitensis strain rev 1 vaccine (rev 1) is administered by the standard method (1-2 x 10(9) viable bacteria injected subcutaneously), it may induce long-lasting serological responses and/or cause abortion in pregnant animals. the conjunctival route considerably reduces these drawbacks. in the present experiment a 1 x 10(8) cfu dose for both ewes and goats conjunctivally vaccinated at mid-pregnancy was tested for innocuousness (outcome of pregnancy, contamination of unvaccinated con ... | 1992 | 1610080 |
| rapid diagnosis of brucella melitensis in blood: some operational characteristics of the bact/alert. | a clinical isolate of brucella melitensis was detected in a blood culture with the bact/alert after an incubation period of 2.8 days. dilution studies revealed an inverse linear relationship between the log of the initial concentration of the organism and the time to detection of a positive result. reproducibility studies demonstrated a mean detection time of 48 +/- 1 h in 8 of 10 replicates seeded with a stock containing 10(2) cfu/ml. | 1992 | 1734057 |
| [brucellar pericarditis: 2 different forms of presentation for an unusual etiology]. | two cases of brucella melitensis pericarditis are reported. pericardial involvement was the first and almost only manifestation of brucellosis in the first patient while in the second, a significant pericardial effusion was discovered on a routine echocardiogram performed in a patient with clinically florid brucellosis. some etiopathogenic aspects of this uncommon etiology are discussed. | 1991 | 1759029 |
| brucellosis: an unusual diagnosis for a seronegative patient with abscesses, osteomyelitis, and ulcerative colitis. | a 16-year-old girl developed multiple subcutaneous abscesses, osteomyelitis, and severe colitis. on the patient's second admission, a single blood culture--and, subsequently, a specimen of pus--yielded brucella melitensis biovar 1. a second set of serologic tests, including the rose bengal test, the standard tube agglutination test, the cf test, and coombs' test, were all negative for brucella on the patient's second admission and 1 month later. however, a lymphocyte proliferation assay with ext ... | 1991 | 1775834 |
| brucella melitensis rev. 1 living attenuated vaccine: stability of markers, residual virulence and immunogenicity in mice. | five commercial brucella melitensis rev. 1 vaccines from different sources were compared to the original elberg rev. 1 strain, in vitro for classic markers and in vivo in mice, for residual virulence and immunogenicity. because colonies of several morphology types (smooth, non-smooth) were isolated from the vaccines, representative substrains were purified to study their in vitro and in vivo activities either at once (16 strains), or after storage by subculture (12 strains) and by lyophilization ... | 1991 | 1797046 |
| characterization of smooth brucella lipopolysaccharides and polysaccharides by monoclonal antibodies. | two mouse monoclonal antibodies (mab) generated to the m antigen of brucella melitensis 16m were analysed. binding profiles of both monoclonals were established by a competitive enzyme-linked immunosorbent assay using chemically defined lipopolysaccharides, o polysaccharides and native hapten polysaccharides from b. melitensis 16m, b. abortus 544 and yersinia enterocolitica o:9. using this assay, significant differences in the reactivity of both antibodies were found with a and m antigens from b ... | 1991 | 1805311 |
| [effect of the extraction of lps (r) on the demonstration of the serological response in acute brucellosis by immunoblotting]. | in order to establish the possible influence of brucella melitensis rough strains protein extracts contamination using lps (r) in the immunoblotting evaluation of serologic response, we have studied sera samples from 41 patients with acute brucellosis before treatment was started and also from 62 healthy individuals (control group). a whole-cell b. melitensis 115 strain extract with an without lps (r) was used. extraction was carried using ether-chloroform of oil-phenol. overall, more than 50% o ... | 1991 | 1805947 |
| induction of antibody and protective responses in mice by brucella o-polysaccharide-bsa conjugate. | a brucella conjugate vaccine was synthesized by covalently coupling the o-polysaccharide (ps) obtained from brucella melitensis 16m, to bovine serum albumin (bsa). the conjugate (ps-bsa) was non-toxic for mice. when injected at two doses (10 or 1 micrograms) in mice, it induced the production of antibodies and the boost was shown to be efficient if administered after 10 weeks. immunization of mice with 10 micrograms of conjugate conferred significant protection against virulent challenge with b. ... | 1991 | 1811374 |
| brucellosis as a cause of backache. | prospective screening of 173 patients with complaints of backache and a history of ingestion of raw milk yielded 21 patients suffering from brucella spondylitis. the overall incidence of brucellosis was 28 in 10,647 patients; 14 out of 21 (67%) presented with acute signs and symptoms of brucella spondylitis and six patients required hospitalization. the majority of the patients had a positive titre of brucella melitensis and abortus. in 42% of the patients radiographs of the thoraco-lumbar spine ... | 1991 | 1836289 |
| [phenol-insoluble vaccine against human brucellosis: evaluation of the immunogenic power in guinea pigs]. | a phenol insoluble extract vaccine proposed to immunize men against brucellosis was tested for its ability in protecting guinea pigs against challenge with virulent brucella abortus strain 2308. living attenuated brucella abortus strain 19 and b. melitensis rev. 1 were included in the experiment for comparison. ninety three animals were vaccinated in each group and subdivided in subgroups of 31 for challenge with 10(4), 10(3) and 10(2) colony forming units of virulent b. abortus 2308. a global a ... | 1991 | 1843392 |
| [serological survey of small ruminants in 4 districts of niger]. | in 1990, a serosurvey on 1,474 small ruminants was conducted in four districts of niger (maradi, zinder, diffa and dosso). the epidemiology and seroprevalence of eight diseases were studied: brucella melitensis and b. ovis brucellosis, chlamydiosis, coxiellosis, contagious caprine pleuropneumonia, type a pasteurellosis, peste des petits ruminants, rift valley fever, crimée-congo haemorragic fever. the main health problem for the development of small ruminants farming, seems to be both pasteurell ... | 1991 | 1843821 |
| [fever, swelling of testis and epididymis, poly-lymphadenopathy, splenomegaly]. | an 18-year-old turkish farmer was admitted to the hospital because of fever, painful scrotal swelling, generalized lymph-node enlargement and splenomegaly. after an incubation period of six days brucella melitensis was isolated in blood culture. the diagnosis of acute brucellosis with lymphadenopathy, splenomegaly and epididymitis was made. he was treated with doxycycline and rifampicin for six weeks and recovered completely. three months after therapy he felt well. a relapse did not occur. | 1991 | 1871475 |
| in vitro activity of lomefloxacin, a difluorinated quinolone, compared with other antimicrobials. | a total of 3,144 clinical isolates from 3,011 consecutive patients were tested against lomefloxacin by the agar dilution method. they consisted of 1,380 isolates of enterobacteriaceae, 527 pseudomonads, 47 haemophilus influenzae, 53 acinetobacter, 42 brucella melitensis, 903 staphylococci and 192 strains of enterococci. in vitro activity of lomefloxacin was compared with ciprofloxacin, norfloxacin, beta-lactams and aminoglycosides. over 98% of enterobacteriaceae were susceptible to lomefloxacin ... | 1991 | 1889304 |
| infections in british clinical laboratories, 1988-1989. | during 1988-89 this continuing survey showed 18 infections in the staff of laboratories reporting from 166 centres, representing 21,756 person-years of exposure. shigella and other bowel infections (one caused by s typhi) predominated, affecting 11 microbiology medical laboratory scientific officers. three shigella infections originated from quality control samples. pulmonary tuberculosis affected four workers, including two mortuary technicians, but without detected occupational exposure to myc ... | 1991 | 1890201 |
| [sensitivity of brucella melitensis to various antibiotics, drug preparations and their combinations]. | in vitro activity of 13 antibiotics and drug preparations as well as some of their combinations was studied with respect to brucella melitensis. it was shown that the strains of b. melitensis were sensitive to sisomicin, amikacin, gentamicin, cefotaxime, rifaprim +, nitroxoline and trimethoprim. variations in the doxycycline sensitivity depending on the strain were observed. the strains of b. melitensis were resistant to cephalexin, phosphomycin, chinoxydine, dioxydine and oxolinic acid. combina ... | 1991 | 1892428 |
| [a case from practice (227). bang disease (brucella melitensis) with-- spondylitis c7/th1]. | 1991 | 1947557 | |
| in vitro susceptibility of brucella melitensis to antibiotics. | the in vitro susceptibilities of 86 recent clinical isolates of brucella melitensis to minocycline, streptomycin, co-trimoxazole, rifampin, and six fluoroquinolones were determined. minocycline exhibited the lowest mic and was followed by rifampin and streptomycin. among the quinolones, win 57273 and ciprofloxacin were the most active agents. no antibiotic combination of these agents exhibited synergy against 15 selected isolates. in killing rate experiments, streptomycin exhibited the most rapi ... | 1991 | 1952869 |
| a rare cause of colitis--brucella melitensis. report of a case. | documentation of gastrointestinal lesions in brucella infections is sparse. a case of brucella melitensis type 3 infection accompanied by erosive lesions of the colon, observed by endoscopy and histopathologic examination, is reported. such gastrointestinal lesions have not been described since 1934. before 1934 only postmortem observations are recorded. | 1991 | 1993419 |
| physical map of the brucella melitensis 16 m chromosome. | we present the first restriction map of the brucella melitensis 16 m chromosome obtained by southern blot hybridization of spei, xhoi, and xbai fragments separated by pulsed-field gel electrophoresis. all restriction fragments (a total of 113) were mapped into an open circle. the main difficulty in mapping involved the exceedingly high number of restriction fragments, as was expected considering the 59% g + c content of the brucella genome. several cloned genes were placed on this map, especiall ... | 1991 | 2007548 |
| outbreak of brucella melitensis among microbiology laboratory workers in a community hospital. | from may to september 1988, eight employees of a microbiology laboratory developed acute brucellosis (attack rate, 31%). seven of the eight affected employees had clinical illness ranging from a nonspecific, flulike illness to severe hepatitis. blood cultures obtained from five of the affected employees (63%) were positive for brucella melitensis, biotype 3. comparison of cases and controls showed that there were no risk factors besides employment in the laboratory. based on work locations, assi ... | 1991 | 2007637 |
| antibacterial activity of fce 22101 against relatively resistant blood culture isolates. | in vitro antibacterial activity of the new penem fce 22101 was tested against blood culture isolates from 1,374 consecutive patients in a major tertiary care referral center in saudi arabia. many of the isolates were significantly more resistant to commonly used beta lactams. of the 809 members of enterobacteriaceae tested all but 14 isolates of enterobacter were susceptible to fce 22101 with an mic range of 0.12-8.0 micrograms/ml. it inhibited all the 183 isolates of staphylococcus aureus, both ... | 1991 | 2013238 |
| bilateral mammary abscess and uveitis caused by brucella melitensis--report of a case. | soft tissue locations of brucella are a rare finding. we report the isolation of brucella melitensis from a suspected breast tumour of a woman who also showed signs of uveitis. three weeks after surgical drainage and despite antimicrobial therapy a new abscess developed on the other breast whose culture also yielded brucella. as observed, unusual localization of brucellosis without previous penetrating injury may be the only manifestation of chronic infection. | 1991 | 2013508 |
| [brucella endocarditis on starr aortic valve prosthesis]. | the authors report the case of a 22 year old patient with brucella endocarditis on a starr-edwards aortic valve prosthesis implanted 5 years previously. six blood cultures were positive for brucella melitensis biovar i. antibiotic therapy associating rifampicin and tetracycline and then rifampicin and ofloxacine did not prevent abscess formation and valve dehiscence. the poor haemodynamic status and persistent infection led to replacement of the valve prosthesis. culture of the infected prosthes ... | 1991 | 2021291 |
| comparison of serological tests for brucella melitensis infection in sheep. | a comparative study of the standard tube agglutination test (sat), rose bengal plate agglutination test and counter immuno-electrophoresis (ciep) was made on 647 sera from naturally aborting ewes, orchitic, in-contact and apparently healthy sheep with no history of vaccination against brucellosis. no individual test could detect all the 13 known positive reactors (the foetuses of which yielded brucella melitensis) but by combination of two tests all 13 were positive. the sat detected more reacto ... | 1991 | 2038764 |
| identification of dye-sensitive strains of brucella melitensis. | strains of brucella melitensis biovars 1, 2, and 3 isolated in india, italy, kuwait, saudi arabia, the federal republic of germany, and zimbabwe were inhibited by thionin (20 micrograms/ml) but not by basic fuchsin (20 micrograms/ml) or safranin o (200 micrograms/ml). in other respects the strains were typical of their species and biovars. the existence of dye-sensitive strains of b. melitensis suggests that the present system of classification requires modification. | 1991 | 2056043 |
| brucellosis as a cause of severe colitis. | a 16-yr-old girl presented with osteomyelitis and massive rectal bleeding. colonoscopy revealed severe nonspecific colitis. multiple laboratory investigations failed to disclose the etiology of either the bone or colon infections. empiric treatment with corticosteroids and sulfasalazine resulted in only transient improvement. one month after discharge, her original symptoms recurred. blood and pus cultures at this time yielded brucella melitensis. after tetracycline treatment, the patient recove ... | 1991 | 2058640 |
| [brucella endocarditis of bicuspid aortic valve. surgical treatment with successful result]. | the authors report a case of brucella melitensis endocarditis of a bicuspid aortic valve which caused rapid progression of the hemodynamic signs of aortic stenosis, and was associated with a para-aortic abscess and a pericardial effusion. surgery resulted in correction of the hemodynamic abnormalities and cured the infection: the results were sustained 10 months after operation. this case illustrates the precision of the doppler, echocardiographic diagnosis of the lesions, which was confirmed at ... | 1991 | 2064520 |
| inhibition of the degranulation and myeloperoxidase activity of human polymorphonuclear neutrophils by brucella melitensis. | the degranulation and myeloperoxidase-h2o2-halide activities of human polymorphonuclear leukocytes from healthy donors were tested after co-incubation with either brucella melitensis 16m, staphylococcus aureus or staphylococcus aureus in presence of lipopolysaccharide, protein fraction, native hapten and soluble fractions released at 65 degrees c from smooth strain of brucella melitensis 16m. the degranulation and myeloperoxidase activities of polymorphonuclear leukocytes were significantly high ... | 1991 | 1662050 |
| microbiological test strip (api20ne) identifies brucella melitensis as moraxella phenylpyruvica. | 1991 | 1669878 | |
| brucellosis surveillance. | 1991 | 1669892 | |
| person-to-person transmission of brucella melitensis. | human brucellosis is primarily an occupational hazard in the usa; in the middle east and africa ingestion of contaminated dairy products is an important route of infection. whether human beings can become infected via person-to-person spread is uncertain. during an investigation of a commonsource, laboratory-associated outbreak due to brucella melitensis, biotype 3, the wife of a microbiologist with serologically proven brucellosis became infected. her blood isolate was indistinguishable from th ... | 1991 | 1670649 |
| transmission of brucella melitensis. | 1991 | 1672931 | |
| antigenic relationships of the lipopolysaccharides of escherichia hermannii strains with those of escherichia coli o157:h7, brucella melitensis, and brucella abortus. | clinical isolates of escherichia hermannii which showed serological cross-reaction with polyclonal antisera to the o-polysaccharide portion of the lipopolysaccharide of e. coli o157 strains and with antisera to the o antigens of brucella abortus and b. melitensis were found by chemical and nuclear magnetic resonance analyses to have lipopolysaccharide o chains composed of linear polymers containing 1,2- and 1,3-linked 4-acetamido-4,6-dideoxy-alpha-d-mannopyranosyl (alpha-d-rhap4nac) residues. tw ... | 1990 | 1691146 |
| [cloning of brucella genes in escherichia coli k12 cells and analysis of products of the cloned genes]. | the libraries of brucella melitensis 565 and brucella abortus 99 in escherichia coli cells have been constructed. some clones of escherichia coli producing the specific brucella antigens have been found in immunological tests with brucella antiserum. two strains producing antigens have been characterized, one being from brucella melitensis 565 and another from brucella abortus 99 clone libraries . both strains synthesize two antigens that were studied by immunoelectrophoresis, immunoblotting aft ... | 1990 | 1701518 |
| probable breast-milk borne brucellosis in a young infant. | a young infant with acute brucellosis is reported. he presented with a septicaemia-like picture. diagnosis was based on a fourfold rise of brucella agglutination titres and a positive blood culture. he had been exclusively breast-fed when his mother developed brucellosis 4 weeks after delivery. it is strongly suspected that the transmission of brucella melitensis to this infant was through the maternal breast-milk. | 1990 | 1703749 |
| [the antigenic relationship between brucella abortus, brucella melitensis and yersinia enterocolitica serotype 0:3 and 0:9]. | antigenic interrelationship between b. abortus, b. melitensis, y. enterocolitica serotype 0:3 and serotype 0:9 were investigated in hyperimmün sera obtained from rabbits, by using agglutination and agglutinin absorbtion tests. agglutination was determined at 1/2560 titer of b. abortus and b. melitensis hyperimmün sera when their own antigens were used. on the other hand, these sera agglutinated y. enterocolitica serotype 0:9 antigen at 1/1280 and 1/320 titer respectively. as it was seen that hig ... | 1990 | 1704479 |
| brucellosis in patients with heart disease: when should endocarditis be diagnosed? | three patients, 2 with congenital valvular heart disease and 1 with a prosthetic aortic valve developed brucellosis. brucella melitensis was isolated from blood of all 3 patients. the clinical and microbiological features suggested brucella endocarditis and following successful antibiotic therapy, no surgery was required. the salient diagnostic features are discussed with emphasis on the management and prognosis of patients with brucella endocarditis. | 1990 | 2073648 |
| [brucellosis in a 3 month-old infant]. | during the course of a bronchiolitis in a 3 month-old child, one blood culture yielded brucella melitensis. the diagnosis of brucellosis is difficult in infancy because the symptoms are nonspecific and the disease may be mild and self-limited. the child received only breast milk and dietetic preparations. so, the detection in the maternal serum of a significant titer of brucella agglutinin enabled us to consider the very rare human-to-human transmission from mother to child. because the efficacy ... | 1990 | 2078109 |