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Rapid determination of perv copy number from porcine genomic DNA by real-time polymerase chain reaction.Here, we report the quantification of porcine endogenous retrovirus (PERV) copy numbers using real time PCR. After generating standard curves using plasmid DNA, copy numbers were determined for PERV pol and for a housekeeping gene, porcine estrogen receptor2 (ER2) with the same amount of genomic DNA. Using this method, we examined 6 pig breeds in Korea including two breeds of miniature pig, one domestic pig from Jeju, and imported pig breeds, Duroc, Landrace, and Yorkshire. All breeds showed PER ...201122132811
molecular study of porcine circovirus type 2 circulating in portugal.porcine circovirus type 2 (pcv2) belongs to the genus circovirus of the circoviridae family and has been associated with a disease named postweaning multisystemic wasting syndrome (pmws). in portugal the disease caused severe problems mainly between 1995 and 2003, but is nowadays less evident probably as the result of vaccination programmes initiated in 2007. this study reports the molecular analysis of 22 pcv2 strains obtained from domestic pigs originated from different regions of portugal. on ...201121925289
comparative study between trimetazidine and ice slush hypothermia in protection against renal ischemia/reperfusion injury in a porcine model.the aim of the study was to compare the effects of renal ice slush hypothermia and the use of trimetazidine in the protection against ischemia/reperfusion (i/r) injury.201122099266
Endoscopic full-thickness resection in the colon by using a clip-and-cut technique: an animal study.Although endoscopic resection techniques have been established for definitive therapy of mucosal neoplasia, complete histopathological assessment or resection of subepithelial lesions is not reliably possible.201121944313
Hepatitis E virus genotype 3f in pigs in New Caledonia.Hepatitis E virus (HEV) is highly prevalent in farm pigs worldwide and an increasing body of data from industrialised countries suggests that it is an agent of a porcine zoonosis.201122103949
myocardial bridges in domestic pig--morphological aspects.the morphology of myocardial bridges (mb) in the heart of the domestic pig remain an open issue. despite numerous analyses of the subject, many controversies still exist. opinions also differ when the influence of the mb on haemodynamic processes in the coronal vessel system is concerned. in the examined group of 150 domestic pig's hearts, the length of the detected mb varied from 1.8 to 39.7 mm while their thickness amounted to 0.8 - 4.7 mm. both the longest and the thickest bridges were connec ...201121957735
Development and evaluation of a selective medium for Brucella suis.A new selective medium, named LNIV-M, has been developed for isolation of Brucella suis. In this work, we evaluated the growth of B. suis reference and field strains from domestic pigs in different basal media and the susceptibility to different antibiotics contained in the currently used Farrell's and modified Thayer-Martin media. We also determined the efficacy of LNIV-M and its diagnostic performance for isolating B. suis from wild boar tissue samples. A total of 1649 samples from 918 hunter- ...201121968103
robust spinal motor neuron transduction following intrathecal delivery of aav9 in pigs.adeno-associated viral vector 9 (aav9) has recently been shown to penetrate the blood-brain barrier via intravascular administration, making it a good candidate for diffuse gene delivery. however, the potential side effects of systemic delivery are unknown. intrathecal viral vector administration may be more invasive than intravenous injections, but it requires far less vector and it can be performed on an outpatient basis, making it an ideal route of delivery for clinical translation. a total o ...201121918551
differential decomposition patterns in charred versus un-charred remains.although researchers have examined many aspects of fire modification, the rate and pattern of decomposition in charred remains have not been studied previously. this study utilized 48 domestic pigs, divided into 24 charred (head, neck, and limbs burned to crow-glassman level 1 and torso to level 2) and 24 un-charred pig carcasses. decomposition of control carcasses was scored at 50 accumulated degree days (add) intervals, and charred carcasses were also observed and photographed at this time. a ...201121923798
similar rates of chromosomal aberrant secondary oocytes in two indigenous cattle (bos taurus) breeds as determined by dual-color fish.in vitro-matured metaphase ii (mii) oocytes with corresponding first polar bodies (i pb) from two indigenous cattle (bos taurus) breeds have been investigated to provide specific data upon the incidence of aneuploidy. a total of 165 and 140 in vitro-matured mii oocytes of the podolian (po) and maremmana (ma) breeds, respectively, were analyzed by fluorescence in situ hybridization using xcen and five chromosome-specific painting probes. oocytes with unreduced chromosome number were 13.3% and 6.4 ...201122056011
Influence of castration-induced sex hormone deficiency on serum lipid levels and the genes expression in male pigs.It has been known that the changes in gonadal steroids are closely associated with adipose tissue metabolism. Domestic pigs have been a well-recognized experimental animal in biomedical research because of their similarity to humans in body size and other physiological/anatomical features. The aims of this study were to investigate the influence of castration-induced sex hormone deficiency on serum lipid levels and the genes expression of key enzymes associated with lipogenic and lipolytic proce ...201121932170
oct4-enhanced green fluorescent protein transgenic pigs: a new large animal model for reprogramming studies.the domesticated pig has emerged as an important tool for development of surgical techniques, advancement of xenotransplantation, creation of important disease models, and preclinical testing of novel cell therapies. however, germ line-competent pluripotent porcine stem cells have not yet been derived. this has been a major obstacle to genetic modification of pigs. the transcription factor oct4 is essential for the maintenance of pluripotency and for reprogramming somatic cells to a pluripotent ...201121126163
a prospective randomized comparison of traditional laparoendoscopic single-site surgery with needlescopic-assisted laparoscopic nephrectomy in the porcine model.abstract background and purpose: laparoendoscopic single-site (less) surgery produces virtually no scar but is technically challenging because of the loss of triangulation. the objective of this study is to compare classic transumbilical less nephrectomy with needlescopic-assisted laparoscopy (nal) surgery. in doing so, we evaluated whether the addition of a single 2-mm subcostal port could restore triangulation while not jeopardizing recovery or cosmetic outcome in the porcine model. materials ...201121631303
treatment of major vein injury with the hemostatic fleece tachosil by interposing a peritoneal patch to avoid vein thrombosis: a feasibility study in pigs.vein lacerations in awkward locations are difficult to repair and carry high mortality. the hemostatic fleece, tachosil, is effective in preventing intraoperative bleeding in different settings, but has not been recommended for use in large vein injury. tachosil with a peritoneal patch interposed to avoid vein thrombosis has been reported as a method to obtain hemostasis in vein laceration, but further studies of this method are needed.201121633573
[laparoscopic pancreatic resections in experimental setting and clinical practice].pancreatic fistula is a major postoperative complication after pancreatic resection. one of the main risk factors of developing the pancreatic fistula after distal pancreatectomy is the method employed for the management of the pancreatic remnant.201121634100
differential resistance of mammalian sperm chromatin to oxidative stress as assessed by a two-tailed comet assay.protamines of eutherian species are cysteine-rich molecules that become cross-linked by disulfide bonds during epididymal transit, whereas the protamines of most marsupial species lack cysteine residuals. the present study made use of the differences in protamine structure between eutherian and metatherian mammal spermatozoa to examine the comparative resistance of sperm dna to oxidative damage in three eutherian species (mus musculus, homo sapiens, sus domesticus) and three metatherian species ...201121635811
suture-free anastomosis of the colon. experimental comparison of two cyanoacrylate adhesives.we explored the potential of two cyanoacrylate tissue adhesives for constructing colonic anastomoses.201121229329
identification of a second gene associated with variation in vertebral number in domestic pigs.the number of vertebrae in pigs varies and is associated with body size. wild boars have 19 vertebrae, but european commercial breeds for pork production have 20 to 23 vertebrae. we previously identified two quantitative trait loci (qtls) for number of vertebrae on sus scrofa chromosomes (ssc) 1 and 7, and reported that an orphan nuclear receptor, nr6a1, was located at the qtl on ssc1. at the nr6a1 locus, wild boars and asian local breed pigs had the wild-type allele and european commercial-bree ...201121232157
microrna expression profiling of the porcine developing brain.micrornas are small, non-coding rna molecules that regulate gene expression at the post-transcriptional level and play an important role in the control of developmental and physiological processes. in particular, the developing brain contains an impressive diversity of micrornas. most microrna expression profiling studies have been performed in human or rodents and relatively limited knowledge exists in other mammalian species. the domestic pig is considered to be an excellent, alternate, large ...201121253018
distinctive differences in long terminal repeat sequences between γ1 endogenous retroviruses of african and eurasian suid species.diversity of long terminal repeats (ltrs) from γ1 endogenous retroviruses (ervs) was analysed by dna sequencing in 10 species of the family suidae (suids, pigs and hogs). phylogenetic analysis separated ltr sequences into two groups which correlated approximately with either the previously described cluster i and iii, or the clusters ii, iv and v. interestingly, a specific ltr exhibiting a novel molecular rearrangement was identified exclusively within african host species when compared to ltrs ...201121256982
shen-fu injection attenuates postresuscitation myocardial dysfunction in a porcine model of cardiac arrest.to investigate the effect of shen-fu injection (sfi) for the management of postresuscitation myocardial dysfunction in a porcine model of cardiac arrest. ventricular fibrillation was induced electrically in anesthetized domestic swine. after 4 min of untreated ventricular fibrillation, cardiopulmonary resuscitation was initiated. sixteen successfully resuscitated pigs were randomized to receive a continuous infusion of either sfi (0.24 mg/min) or saline placebo for 6 h, beginning 15 min after re ...201121263380
differential mrna expression of genes in the porcine adrenal gland associated with psychosocial stress.to gain insight into the adrenal stress response, we analysed differential mrna expression of genes associated with psychosocial stress in the pig (sus scrofa domestica). various levels of psychosocial stress were induced by mixing groups of unfamiliar pigs with different aggressiveness. we selected two experimental groups for comparison, each comprising eight animals, which differed significantly in aggressive behaviour and plasma cortisol levels. to identify differentially expressed genes, we ...201121266515
genetic characterisation of african swine fever viruses from recent and historical outbreaks in sardinia (1978-2009).three discrete regions of the african swine fever virus (asfv) were analysed in the genomes of a wide range of isolates collected from wild and domestic pigs in sardinia, over a 31-year period (1978-2009). the analysis was conducted by genotyping based on sequence data from three single copy asf genes. the e183l gene encoding the structural protein p54 and part of the gene encoding the p72 protein were used to delineate genotypes, before intra-genotypic resolution of viral relationships by analy ...201121373994
plasma and tissue levels of neuropeptide y in experimental septic shock: relation to hemodynamics, inflammation, oxidative stress, and hemofiltration.neuropeptide y (npy), a potent vasoconstrictor released from the sympathetic nerves, has been suggested to counterbalance sepsis-induced vasodilation. thus, the changes in plasma and tissue npy concentrations in relation to hemodynamic variables and inflammatory markers in a porcine model of moderate septic shock were investigated. susceptibility of npy to be removed by continuous hemofiltration in two settings has been also studied. thirty-four domestic pigs were divided into five groups: (i) c ...201121375544
developmental changes affecting lectin binding in the vomeronasal organ of domestic pigs, sus scrofa.this study investigated the developmental changes of glycoconjugate patterns in the porcine vomeronasal organs (vnos) and associated glands (jacobson's glands) from prenatal (9 weeks of gestation) and postnatal (2 days after birth) to the sexually mature stage (6 months old). the vno of pigs (sus scrofa) was examined using the following: dolichos biflorus agglutinin (dba), bandeiraea simplicifolia agglutinin isolectin b4 (bsi-b4), triticum vulgaris agglutinin (wga), ulex europaeus agglutinin i ( ...201121376374
mycobacterium caprae infection in livestock and wildlife, spain.mycobacterium caprae is a pathogen that can infect animals and humans. to better understand the epidemiology of m. caprae, we spoligotyped 791 animal isolates. results suggest infection is widespread in spain, affecting 6 domestic and wild animal species. the epidemiology is driven by infections in caprids, although the organism has emerged in cattle.201121392452
elevated japanese encephalitis virus activity monitored by domestic sentinel piglets in thailand.twenty-nine domestic piglets from pig farms located in three provinces of thailand between 2003 and 2004 were used as sentinel animals for japanese encephalitis virus (jev) circulation. piglets were used as sentinel to underline, on one hand, the role of domestic pigs as jev amplifying host and, on another hand, to point out the interest of using sentinel animals for japanese encephalitis surveillance. jev activity was demonstrated through i/ antibody detection using a specific elisa test for th ...201121395418
a novel isolation method of brucella species and molecular tracking of brucella suis biovar 2 in domestic and wild animals.brucella suis biovar 2 is the most common aetiological agent of porcine brucellosis in europe. b. suis biovar 2 is considered to have low zoonotic potential, but is a causative agent of reproductive losses in pigs, and it is thus economically important. the multilocus variable-number of tandem repeats genotyping analysis of 16 loci (mlva-16) has proven to be highly discriminatory and is the most suitable assay for simultaneously identifying b. suis and tracking infections. the aim of this study ...201121474256
xenobiotic metabolizing cytochrome p450 in pig, a promising animal model.the pig has been used as an important animal model for human studies because of its similarity in size, physiology and disease development. however, in contrast to the extensive data available on the cytochrome p450 (cyp) system for humans and rodents, the data related to pig are limited because of, among others, the presence of intra-species differences (domestic pigs and minipigs). the knowledge of the cyp superfamily in a given experimental animal is crucial for pharmacological and toxicologi ...201121476973
voiding prior to parachuting.multiple incidents of isolated bladder ruptures have occurred in the military parachuting community after jumps. the aim of this article is to demonstrate the amount of energy it takes to rupture a distended bladder and encourage prejump voiding.201121481808
variable effects of high-dose adrenaline relative to standard-dose adrenaline on resuscitation outcomes according to cardiac arrest duration.adjustment of adrenaline (epinephrine) dosage according to cardiac arrest (ca) duration, rather than administering the same dose, may theoretically improve resuscitation outcomes. we evaluated variable effects of high-dose adrenaline (hda) relative to standard-dose adrenaline (sda) on resuscitation outcomes according to ca duration.201121482013
genome-wide footprints of pig domestication and selection revealed through massive parallel sequencing of pooled dna.background: artificial selection has caused rapid evolution in domesticated species. the identification of selection footprints across domesticated genomes can contribute to uncover the genetic basis of phenotypic diversity. methodology/main findings: genome wide footprints of pig domestication and selection were identified using massive parallel sequencing of pooled reduced representation libraries (rrl) representing ∼2% of the genome from wild boar and four domestic pig breeds (large white, la ...201121483733
ileorectal bypass performed entirely through a transanal route in a porcine model.background: natural orifice transluminal endoscopic surgery (notes), a new frontier of minimally invasive surgery, uses the body's natural orifices to create an access for surgical procedures. this study aimed to verify the technical feasibility of ileorectal bypass performed entirely through a transanal access. methods: the procedure was performed on 10 domestic pigs, after which they were killed. a transanal endoscopic microsurgery (tem) device and endoscopic and laparoscopic instruments were ...201121484531
partial protection against classical swine fever virus elicited by dendrimeric vaccine-candidate peptides in domestic pigs.we report the immunogenicity of three dendrimeric peptide vaccine candidates for classical swine fever virus (csfv). each dendrimeric construct contained four copies of a b-cell epitope from the e2 glycoprotein of csfv [construct 1: e2 (694-712); 2: e2 (712-727); 3: e2 (829-842)] joined to a t-cell epitope from the ns3 protein (residues 1446-1460). intramuscular immunization of domestic pigs with the different constructs significantly reduced the clinical score after lethal challenge with csfv. ...201121496472
experimental pig model of old myocardial infarction with long survival leading to chronic left ventricular dysfunction and remodeling as evaluated by pet.a pig model of reduced left ventricular (lv) function and remodeling or chronic heart failure with long survival after myocardial infarction (mi) has not been established. the aim of this study was to evaluate the pathophysiologic status of a pig model of old mi using a series of pet studies.201121498524
[extracorporeal cardiac shock wave therapy improved myocardial micro-vascular circulation after acute myocardial infarction at early stage in pigs].to explore the effect of low-energy extracorporeal shock wave therapy to improve myocardial micro-vascular circulation after acute myocardial infarction at the early stage in pig model.201121500558
characterization of a novel porcine enterovirus in domestic pig in hungary.porcine enteroviruses (pevs) of genus enterovirus are small, non-enveloped viruses with single-stranded, positive sense genomic rna, belonging to the family picornaviridae. the discovery of two distinct serotypes (pev9 and 10) was first reported in 1979. despite the sporadic detection and partial genome sequences of these viruses our knowledge about the prevalence and molecular epidemiology of pev types in domestic pigs is very deficient. in this study, we identified a novel pev from fecal sampl ...201121504800
seroprevalence of toxoplasma gondii infection in domestic pigs in durango state, mexico.abstract little is known concerning the prevalence of toxoplasma gondii infection in pigs in mexico. antibodies to t. gondii were determined in 1,077 domestic pigs in durango, mexico using the modified agglutination test (mat). two groups (a, b) of pigs were sampled: group a pigs (n=555) were raised in 3 geographical regions in durango state and group b pigs (n=522) were from sonora state but slaughtered in durango city. overall, antibodies to t. gondii were found in 137 (12.7%) of 1,077 pigs, w ...201121506843
hypothesis on the dual origin of the mammalian subplate.the development of the mammalian neocortex relies heavily on subplate. the proportion of this cell population varies considerably in different mammalian species. subplate is almost undetectable in marsupials, forms a thin, but distinct layer in mouse and rat, a larger layer in carnivores and big-brained mammals as pig, and a highly developed embryonic structure in human and non-human primates. the evolutionary origin of subplate neurons is the subject of current debate. some hypothesize that sub ...201121519390
detection and phylogenetic characterization of human hepatitis e virus strains, czech republic.to determine the origin of hepatitis e virus in the czech republic, we analyzed patient clinical samples. five isolates of genotypes 3e, 3f, and 3g were obtained. their genetic relatedness with czech strains from domestic pigs and wild boars and patient recollections suggest an autochthonous source likely linked to consumption of contaminated pork.201121529412
successful transfection of genes using aav-2/9 vector in swine coronary and peripheral arteries.background: gene therapy has attracted attention for its potential to treat several cardiovascular diseases. the use of adeno-associated viral (aav) vectors to facilitate therapeutic gene transfer to suppress intimal hyperplasia is a promising concept. the objective of this study was to analyze the in vivo transduction of a novel recombinant aav-2/9 vector with sm22α promoter, containing β-galactosidase gene (lacz) or green fluorescent protein (gfp) as reporter genes, to the medial layer smooth ...201121529824
long-term evaluation of a selective retrograde coronary venous perfusion model in pigs (sus scrofa domestica).the lack of suitable target vessels remains a challenge for aortocoronary bypass grafting in end-stage coronary heart disease. this study aimed to investigate the arterialization of cardiac veins as an alternative myocardial revascularization strategy in an experimental long-term model in pigs. selective retrograde perfusion of a coronary vein (aorta to coronary vein bypass, retrobypass) before ligation of the ramus interventricularis paraconalis (equivalent to the left anterior descending arter ...201121535926
pcr-based molecular characterization and insilico analysis of food-borne trematode parasites paragonimus westermani, fasciolopsis buski and fasciola gigantica from northeast india using its2 rdna.food-borne fluke infections/trematodiases are emerging as a major public health problem worldwide with over 40 million people affected and over 10% of world population at risk of infection. the major concentration of these infections is in southeast asian and western pacific regions, where the epidemiological factors (including the prevalent socio-cultural food habits) are conducive for transmission of these infections. the preponderance of these infections is usually in food deficit poor commun ...201121544167
evaluation of post-farm-gate passive surveillance in swine for the detection of foot and mouth disease in australia.pigs are considered high risk for the introduction and spread of foot and mouth disease (fmd) in australia. one of the most likely pathways of introduction of fmd into australia would be through the illegal importation of fmd-contaminated meat, which is then fed to feral or domestic pigs. locations where animals from different origins are commingled, such as livestock markets and abattoirs, pose a risk for disease spread. early detection of exotic diseases at these locations is crucial in limiti ...201121546105
protection of european domestic pigs from virulent african isolates of african swine fever virus by experimental immunisation.african swine fever (asf) is an acute haemorrhagic disease of domestic pigs for which there is currently no vaccine. we showed that experimental immunisation of pigs with the non-virulent ourt88/3 genotype i isolate from portugal followed by the closely related virulent ourt88/1 genotype i isolate could confer protection against challenge with virulent isolates from africa including the genotype i benin 97/1 isolate and genotype x uganda 1965 isolate. this immunisation strategy protected most pi ...201121549789
effects of sustained abdominal aorta compression on coronary perfusion pressures and restoration of spontaneous circulation during cardiopulmonary resuscitation in swine.objectives: the present study was undertaken to explore whether sustained abdominal aorta compression-cardiopulmonary resuscitation (saac-cpr), as a means, can raise coronary perfusion pressure (cpp) as well as restoration of spontaneous circulation (rosc) during cpr. in the present study, we hypothesised that saac-cpr elevates cpp during cpr and improves rosc, without causing liver laceration. methods: animals were randomised into one of two groups (standard cpr and saac-cpr). ten domestic swin ...201121550162
integration between a percutaneous implant and the porcine small bowel.inflammatory bowel diseases, cancer or trauma may require removal of all or part of the intestines, leaving the patient with a need to wear external stoma appliances for collection of bowel contents. by connecting the small bowel to a percutaneous port, equipped with a sealing lid, a fully continent and leak proof stoma can be created without a need for permanently wearing stoma appliance. the prerequisites for a connection between a permanent, transabdominal implant and a visceral organ are lar ...201121563306
genotyping of mycoplasma hyopneumoniae in wild boar lung samples.mycoplasma hyopneumoniae is the etiologic agent of enzootic pneumonia (ep), an important cause of disease-associated losses in swine production and a role of wild boar in recurrent infections can be supposed. genotypes of m. hyopneumoniae from wild boar are unknown but could indicate its role as a potential reservoir. therefore, 34 lung samples being pcr-positive for m. hyopneumoniae from wild boar from the geneva region in switzerland were assayed by genotyping using the p146 and multi-locus se ...201121571452
thermodilution-derived indices for assessment of left and right ventricular cardiac function in normal and impaired cardiac function.objective:: the aim of this study was to assess whether thermodilution-derived parameters of right and left ventricular cardiac function (right ventricular ejection fraction, global ejection fraction, cardiac function index) are able to track changes of cardiac contractile function and whether they are influenced by substantial preload reduction. design:: prospective animal study. setting:: university-affiliated animal research laboratory. subjects:: domestic pigs. interventions:: sixteen domest ...201121572331
emergence of classical swine fever virus in israel in 2009.classical swine fever (csf) re-emerged in israel in february 2009 after an absence of 62years. the outbreak occurred on a domestic pig farm in northern israel and affected domestic pigs and wild boar. on the basis of phylogenetic analysis of a 190 base pair fragment of the e2 glycoprotein gene, the israeli csf virus strain belonged to genotype 2.1 and was genetically most similar to a chinese csf virus strain.201121592830
laparoscopic fixation of biologic mesh at the hiatus with fibrin or polyethylene glycol sealant in a porcine model.background: the objective of this study was to determine the acute and chronic fixation strengths achieved by fibrin or polyethylene glycol (peg) sealants to secure biologic mesh at the esophageal hiatus in a porcine model. methods: for this study, 32 female domestic pigs were divided into four groups of 8 each. the four groups respectively received acute fibrin sealant, acute peg sealant, chronic fibrin sealant, and chronic peg sealant. laparoscopically, a 5.5 × 8.5-cm piece of biodesign surgis ...201121594740
quantitative assessment of cardiac output and left ventricular function by noninvasive phase-contrast and cine mri: validation study with invasive pressure-volume loop analysis in a swine model.purpose: to validate noninvasive cardiac output measurements of phase-contrast magnetic resonance imaging (pc-mri) and cine mri using an invasive pressure-volume (pv) loop technique on a swine model. materials and methods: we compared three methods for evaluating cardiac function at rest and under pharmaceutical low-dose inotropic infusion conditions: 1) phase-contrast mri, 2) cine mri, and 3) pv loop relationship. these measurements were made in 14 domestic pigs under rest conditions. identical ...201121598342
[artyfechinostomum sufratyfex lane, 1915 (trematoda:echinostomatidae), a parasite of mammals in southern vietnam].trematoda artyfechinostomumn sufratyfex lane, 1915 was found in different mammal species of southern vietnam. original measurements of this species from domestic pig, black rat, and asian palm civet are given.201121598669
evolution of endogenous retroviruses in the suidae: evidence for different viral subpopulations in african and eurasian host species.abstract: background: porcine endogenous retroviruses (pervs) represent remnants of an exogenous form that have become integrated in the domestic pig (sus scrofa) genome. although they are usually inactive, the capacity of gamma1 ervs to infect human cells in vitro has raised concerns about xenotransplantation because the viruses could cross the species barrier to humans. here we have analyzed the evolution of gamma1 ervs in ten species of suidae (suids, pigs and hogs) from eurasia and africa us ...201121609472
parenteral aluminum induces liver injury in a newborn piglet model.parenteral nutrition-associated cholestasis remains a significant problem, especially for the surgical neonate. aluminum is a toxic element known to contaminate parenteral nutrition. we hypothesize that parenterally administered aluminum causes liver injury similar to that seen in parenteral nutrition-associated cholestasis.201121616246
the consumption of glucose during ex vivo lung perfusion correlates with lung edema.ex vivo lung perfusion (evlp) has been recently proposed to recondition organs before transplantation from donors with marginal or unacceptable features. the aim of our investigation was to explore glucose consumption during evlp.201121620034
long-term treatment with nifedipine suppresses coronary hyperconstricting responses and inflammatory changes induced by paclitaxel-eluting stent in pigs in vivo: possible involvement of rho-kinase pathway.aims accumulating evidence indicates that coronary vasoconstricting responses are enhanced at the edges of coronary segment implanted with a drug-eluting stent (des) compared with a bare-metal stent (bms) in humans. we have recently demonstrated that rho-kinase pathway plays an important role in des-induced coronary hyperconstricting responses associated with inflammatory changes in pigs in vivo. this study examined whether long-term treatment with calcium channel blocker suppresses des-induced ...201121624903
development of lidocaine-coated microneedle product for rapid, safe, and prolonged local analgesic action.purpose: to demonstrate rapid (~1-ámin) lidocaine delivery using 3m's solid microstructured transdermal system (smts) for prolonged, local analgesic action. methods: polymeric microneedles were fabricated via injection molding and then dip-coated using an aqueous lidocaine formulation. the amount of lidocaine coated onto the microneedles was determined by high performance liquid chromatography (hplc). to assess drug delivery and dermal pharmacokinetics, lidocaine-coated microneedles were inserte ...201121735335
pseudorabies virus in wild swine: a global perspective.suid herpesvirus 1 (suhv1, syn. aujeszky's disease virus [adv] or pseudorabies virus [prv]), which belongs to the family herpesviridae, subfamily alphaherpesvirinae, genus varicellovirus is the causative agent of aujeszky's disease (ad, pseudorabies), a notifiable disease, that causes substantial economic losses to the swine industry in countries, where ad is present. members of the family suidae (true pigs) are the only natural hosts for prv, although the virus can infect numerous other mammals ...201121837416
guided bone regeneration in pig calvarial bone defects using autologous mesenchymal stem/progenitor cells - a comparison of different tissue sources.due to donor side morbidity and the absence of osteogenic properties in bone substitutes, there is a growing need for an alternative to traditional bone grafting within the scope of tissue engineering. this animal study was conducted to compare the in-ávivo osteogenic potential of adipose-derived (ad), periosteum-derived (pd) and bone marrow-derived (bm) mesenchymal stem/progenitor cells (msc). autologous mesenchymal stem/progenitor cells of named tissue origin were induced into osteogenic diffe ...201121723141
non-contiguous finished genome sequence of bacteroides coprosuis type strain (pc139).bacteroides coprosuis whitehead et al. 2005 belongs to the genus bacteroides, which is a member of the family bacteroidaceae. members of the genus bacteroides in general are known as beneficial protectors of animal guts against pathogenic microorganisms, and as contributors to the degradation of complex molecules such as polysaccharides. b. coprosuis itself was isolated from a manure storage pit of a swine facility, but has not yet been found in an animal host. the species is of interest solely ...201121677860
influence of the halothane gene (hal) on pork quality in two commercial crossbreeds.we evaluated the effect of the halothane (hal) gene on the quality of pork in domestic pigs. half-carcasses from two different commercial pig (sus domestica) crossbreeds were analyzed, 46 of which were homozygous dominant (hal(nn)) and 69 of which were heterozygous (hal(nn)) for the halothane gene. the measures included backfat thickness, lean meat percentage, carcass weight, ph 24 h after slaughtering, color, and drip loss; dna was extracted from the haunch muscle. swine with the hal(nn) genoty ...201121823098
seroepidemiologic survey for chlamydia suis in wild boar (sus scrofa) populations in italy.we used serology to estimate the prevalence of exposure to chlamydiae in italian populations of wild boars (sus scrofa). sera from 173 hunter-killed wild boars harvested during the 2006-2009 hunting seasons in three italian regions were tested for antibodies to chlamydia suis, chlamydophila pecorum, chlamydophila abortus, and chlamydophila psittaci by the microimmunofluorescence test. antibody titers to chlamydiae ôëñ 1:32 were detected in 110 of the 173 samples tested (63.6%). specific reactivi ...201121719838
comparison of the compression anastomosis ring (endocar) with a circular stapled anastomosis in a porcine model.purpose. the aim of this study was to compare characteristics of rectal compression and stapled anastomoses at multiple time points. methods. a total of 50 domestic pigs underwent a rectal anastomosis with a compression device or a circular stapler. they were sacrificed at zero-time, 2 days, 1 week, 1 month, and 3 months. burst and maximal tolerated pressure and sites of failure, internal diameters, and radiographic leak rates were assessed. desmosine (elastin) levels were determined. results. t ...201121742666
expression, immunolocalization and processing of fertilins adam-1 and adam-2 in the boar (sus domesticus) spermatozoa during epididymal maturation.fertilin alpha (adam-1) and beta (adam-2) are integral membrane proteins of the adam family that form a fertilin complex involved in key steps of the sperm-oocyte membrane interaction. in the present work, we analyzed the presence of adam-1 and adam-2 mrnas, the spermatozoa proteins' processing and their sub-cellular localization in epididymal samples from adult boars. adam-1 and adam-2 mrnas were highly produced in the testis, but also in the vas efferens and the epididymis. on immunoblots of s ...201121718510
cartographical analysis of african swine fever outbreaks in the territory of the russian federation and computer modeling of the basic reproduction ratio.african swine fever (asf), have been introduced into the russian federation from transcaucasia countries, has spread widely across the territory of the southern region of russia since 2008. in this work we present an analysis of the spatial and temporal spread of the disease, determine risk factors by means of gis tools and model the dynamics of the epidemic process both within infected premises (farms) and at the between-farm level to estimate the basic reproduction ratio r(0). the analysis all ...201121840611
evaluation of bioequivalency and toxicological effects of three sources of arachidonic acid (ara) in domestic piglets.arachidonic acid (ara) and docosahexaenoic acid (dha) are routinely added to infant formula to support growth and development. we evaluated the bioequivalence and safety of three ara-rich oils for potential use in infant formula using the neonatal pig model. the primary outcome for bioequivalence was brain accretion of ara and dha. days 3-22 of age, domestic pigs were fed one of three formulas, each containing ara at ôê+0.64% and dha at ôê+0.34% total fatty acids (fa). control diet ara was provi ...201121722692
effects of field-manure applications on stratified 17+¦-estradiol concentrations.the occurrence of the manure-borne estrogen, 17+¦-estradiol (e2), was investigated in laboratory and field soils. in the laboratory, e2 was applied to soil to simulate concentrations found in swine (sus scrofa domestica) manure (5000ngl(-1)). the aqueous-extracted e2 dissipated in the soil by 98% within 1h and was not significantly different from background concentrations (18ng l(-1)) for the duration of the experiment (64h). in the field study, soil cores were taken before and several dates aft ...201121696887
immobilization of russian vx skin depots by localized cooling: implications for decontamination and medical countermeasures.the chemical weapon nerve agent known as russian vx (vr) is a potent organophosphorus (op) compound that is much less studied than its vx analogue with respect to toxicity, as well as to the effectiveness of several known countermeasures against it. an anaesthetized domestic swine model was utilized to assess several approaches in mitigating its toxicity, including the utility of cooling vr treated skin to increase the therapeutic window for treatment. the 6h ld(50) for vr topically applied on t ...201121704135
over-the-wire intravascular ultrasound in an animal model of pulmonary hypertension.intravascular ultrasound (ivus) measures prognostically important pulsatile flow indexes in patients with pulmonary hypertension (ph). ivus catheters traditionally require a guiding catheter for placement which can impact hemodynamics in small infants because the guiding catheter renders the atrioventricular valve incompetent.201121675851
[hepatitis e as zoonosis].the hepatitis e virus (hev) the causative agent of hepatitis e, is a non-enveloped rna virus. hev is transmitted through oral consumption of contaminated food and water according to the currently knowledge now be considered as zoonosis. the main reservoir of hev are pigs, boars and deer. for the first time hev was isolated from animals (pigs) in 1997 in the u.s. genetic analysis of strains isolated from pigs showed high similarity to strains hev isolated from humans. this was the first evidence ...201121735829
Early response of mechanically exposed dental pulps of swine to antibacterial-hemostatic agents or diode laser irradiation.The purpose of this study was to compare the effectiveness of an antibacterial and hemostatic agent to diode laser irradiation in the healing of mechanically exposed porcine pulps.201121678669
Genetic differentiation of infected from vaccinated animals after implementation of an emergency vaccination strategy against classical swine fever in wild boar.Oral emergency vaccination against classical swine fever is a powerful tool to control disease outbreaks among European wild boar and thus to safeguard domestic pigs in affected regions. In the past, when virus detection was mainly done using virus isolation in cell culture or antigen enzyme-linked immunosorbent assays, modified live vaccine strains like C-strain "Riems", were barely detectable after oral vaccination campaigns. Nowadays, the use of highly sensitive molecular techniques has given ...201121684092
pandemic a/h1n1/2009 influenza virus in swine, cameroon, 2010.although swine origin a/h1n1/2009 influenza virus (hereafter "ph1n1″) has been detected in swine in 20 countries, there has been no published surveillance of the virus in african livestock. the objective of this study was to assess the circulation of influenza a viruses, including ph1n1 in swine in cameroon, central africa. we collected 108 nasal swabs and 98 sera samples from domestic pigs randomly sampled at 11 herds in villages and farms in cameroon. ph1n1 was isolated from two swine sampled ...201121963416
reston ebolavirus in humans and animals in the philippines: a review.the 2008 reston ebolavirus infection event in domestic pigs has triggered continuing epidemiologic investigations among philippine health and veterinary agencies in collaboration with international filovirus experts. prior to this, there were only 3 known and documented reston ebolavirus outbreaks in nonhuman primates in the world, all traced back to a single geographic source in the philippines in a monkey breeding/export facility. the first one in 1989 was the first-ever ebola virus that emerg ...201121987747
lactocrine programming of female reproductive tract development: environmental connections to the reproductive continuum.for eutherian mammals a continuum of maternal support insures that development of progeny follows an optimal program. beginning in utero, such support extends into the early neonatal period when bioactive factors are communicated from mother to offspring in colostrum/milk. defined as lactocrine signaling, communication of milk-borne bioactive factors from mother to offspring as a consequence of nursing is important for development of somatic tissues, including the female reproductive tract (frt) ...201122033320
detection of amitriptyline, citalopram, and metabolites in porcine bones following extended outdoor decomposition*skeletal remains of a domestic pig were assessed for relative distribution of amitriptyline, citalopram, and metabolites. following acute exposure and outdoor decomposition for 2 years, drugs and metabolites were analyzed in 13 different bones. bones were pulverized following a simple wash procedure, and drugs were extracted by passive incubation in methanol, followed by solid-phase extraction. samples were analyzed by ultra-high performance liquid chromatography (uhplc) and confirmed with gas c ...201122150755
Multi-locus analysis reveals a different pattern of genetic diversity for mitochondrial and nuclear DNA between wild and domestic pigs in East Asia.A major reduction of genetic diversity in mtDNA occurred during the domestication of East Asian pigs. However, the extent to which genetic diversity has been lost in the nuclear genome is uncertain. To reveal levels and patterns of nucleotide diversity and to elucidate the genetic relationships and demographic history of domestic pigs and their ancestors, wild boars, we investigated 14 nuclear markers (including 8 functional genes, 2 pseudogenes and 4 intergenic regions) from 11 different chromo ...201122065995
correlation of angiographic late loss with neointimal proliferation in stents evaluated by oct and histology in porcine coronary arteries.we aimed to evaluate the correlation of angiographic late loss (ll) with the degree of in-stent neointimal proliferation assessed by optical coherence tomography (oct) and histology.201121920339
Nitric oxide involvement in the acrosome reaction triggered by leptin in pig sperm.Nitric oxide (NO) is a signaling molecule produced by intracellular nitric oxide synthase (NOS) enzymes. This free radical appears to affect sperm capacitation, a maturation step preceding acrosome reaction. Recent studies have reported leptin ability to promote capacitation and acrosome reaction in pig male gametes.201121970701
comparison of a lightweight polypropylene mesh (optilene® lp) and a large-pore knitted ptfe mesh (gore® infinit® mesh)-biocompatibility in a standardized endoscopic extraperitoneal hernia model.purpose: the use of a mesh with good biocompatibility properties is of decisive importance for the avoidance of recurrences and chronic pain in endoscopic hernia repair surgery. as we know from numerous experiments and clinical experience, large-pore, lightweight polypropylene meshes possess the best biocompatibility. however, large-pore meshes of different polymers may be used as well and might be an alternative solution. me ...201121989559
some aspects of purinergic signaling in the ventricular system of porcine brain.numerous signaling pathways function in the brain ventricular system, including the most important - gabaergic, glutaminergic and dopaminergic signaling. purinergic signalization system - comprising nucleotide receptors, nucleotidases, atp and adenosine and their degradation products - are also present in the brain. however, the precise role of nucleotide signalling pathway in the ventricular system has been not elucidated so far. the aim of our research was the identification of all three eleme ...201121995888
Histopathological investigation in porcine infected with torque teno sus virus type 2 by inoculation.ABSTRACT: BACKGROUND: Porcine torque teno sus virus (TTSuV) is a small icosahedral and non-enveloped virus which contains a single-stranded (ssDNA), circular and negative DNA genome and infects mainly vertebrates and is currently classified into the 'floating' genus Anellovirus of Circoviridae with two species. Viral DNA of both porcine TTSuV species has a high prevalence in both healthy and diseased pigs worldwide and multiple infections of TTSuV with distinct genotypes or subtypes of the same ...201122171963
experimental infection of bama miniature pigs with a highly virulent classical swine fever virus.currently, larger domestic pigs are only animals widely used in vaccine evaluation and pathogenicity study of classical swine fever virus (csfv). this study was aimed to create an alternative animal experimental infection model of csfv.201121943299
Organization, complexity and allelic diversity of the porcine (Sus scrofa domestica) immunoglobulin lambda locus.We have characterized the organization, complexity, and expression of the porcine (Sus scrofa domestica) immunoglobulin lambda (IGL) light chain locus, which accounts for about half of antibody light chain usage in swine, yet is nearly totally unknown. Twenty-two IGL variable (IGLV) genes were identified that belong to seven subgroups. Nine genes appear to be functional. Eight possess stop codons, frameshifts, or both, and one is missing the V-EXON. Two additional genes are missing an essential ...201122186825
experimental infection of common warthogs (phacochoerus africanus) and bushpigs (potamochoerus larvatus) with classical swine fever virus. i: susceptibility and transmission.an incursion of classical swine fever virus (csfv) into the domestic pig population in south africa, identified in 2005, raised the concern that infection might spread to wildlife species and be maintained in these hosts. this study sought to determine whether two wildlife suidae species present in south africa, the bushpig (potamochoerus larvatus) and the common warthog (phacochoerus africanus), could support productive csfv infection. both species could be infected with csfv and transmitted in ...201121294855
the role of movement restrictions and pre-emptive destruction in the emergency control strategy against csf outbreaks in domestic pigs.classical swine fever (csf) outbreaks in domestic pig herds lead to the implementation of standard control measures according to legislative regulations. ideal outbreak control entails the swift and efficient culling of all pigs on premises detected positive for csf virus. often all pig holdings around the detected cases are pre-emptively destroyed to exclude transmission into the neighbourhood. in addition to these measures, zones are defined in which surveillance and protection measures are in ...201121300412
optical nerve detection by diffuse reflectance spectroscopy for feedback controlled oral and maxillofacial laser surgery.laser surgery lacks haptic feedback, which is accompanied by the risk of iatrogenic nerve damage. it was the aim of this study to investigate diffuse reflectance spectroscopy for tissue differentiation as the base of a feedback control system to enhance nerve preservation in oral and maxillofacial laser surgery.201121310023
neuronal genes for subcutaneous fat thickness in human and pig are identified by local genomic sequencing and combined snp association study.obesity represents a major global public health problem that increases the risk for cardiovascular or metabolic disease. the pigs represent an exceptional biomedical model related to energy metabolism and obesity in humans. to pinpoint causal genetic factors for a common form of obesity, we conducted local genomic de novo sequencing, 18.2 mb, of a porcine qtl region affecting fatness traits, and carried out snp association studies for backfat thickness and intramuscular fat content in pigs. in o ...201121311593
combining information from surveys of several species to estimate the probability of freedom from echinococcus multilocularis in sweden, finland and mainland norway.the fox tapeworm echinococcus multilocularis has foxes and other canids as definitive host and rodents as intermediate hosts. however, most mammals can be accidental intermediate hosts and the larval stage may cause serious disease in humans. the parasite has never been detected in sweden, finland and mainland norway. all three countries require currently an anthelminthic treatment for dogs and cats prior to entry in order to prevent introduction of the parasite. documentation of freedom from e. ...201121314948
assessment of interactions between african swine fever virus, bushpigs (potamochoerus larvatus), ornithodoros ticks and domestic pigs in north-western madagascar.since its introduction in madagascar in 1998, african swine fever (asf) has severely affected national pig production and persists as a common disease in that country. two of its natural hosts in the african continent, the bushpig (potamochoerus larvatus) and tick vectors of the ornithodoros moubata complex, are reported in west and central regions of the island. however, their role in the maintenance and transmission of the virus has been insufficiently studied. in this work, we tried to assess ...201121320295
a long-term serological survey on aujeszky's disease virus infections in wild boar in east germany.summarybetween 1985 and 2008, a total of 102 387 wild boar sera originating from eastern germany covering an area of 108 589 km2 were tested for the presence of aujeszky's disease virus (adv)-specific antibodies. from 1985 until 1991 and from 1992 until 2008, wild boar sera were exclusively investigated using either conventional seroneutralization assays (n=39 621) or commercial gb and full antigen elisas (n=62 766), respectively. spatial-temporal analysis revealed an increasing adv seroprevalen ...201121320372
discovery of hepatitis e: the epidemic non-a, non-b hepatitis 30 years down the memory lane.hepatitis e was first recognised during an epidemic of hepatitis, which occurred in kashmir valley in 1978. the epidemic involved an estimated 52,000 cases of icteric hepatitis with 1700 deaths. the disease had unique clinical and epidemiological features. the epidemic was water-borne with highly compressed epidemic curve. following the epidemic, secondary waves of hepatitis did not occur. clinical profile was characterized by cholestasis in around 20% of patients. the disease predominantly occu ...201121320558
markers of progenitor cell recruitment and differentiation rise early during ischemia and continue during resuscitation in a porcine acute ischemia model.clinical administration of bone marrow-derived stem cells in the setting of acute myocardial infarction (ami) leads to improved left ventricular ejection fraction. thymosin beta-4 (tb4) and vascular endothelial growth factor (vegf) are linked to adult epicardial progenitor cell mobilization and neovascularization and is cardioprotective after myocardial ischemia. this study investigated the time course of tb4 and vegf during ami, cardiac arrest, and resuscitation. fifteen anesthetized and instru ...201121332365
vascular access port implantation and serial blood sampling in a gottingen minipig (sus scrofa domestica) model of acute radiation injury.threats of nuclear and other radiologic exposures have been increasing, but no countermeasure for acute radiation syndrome has been approved by regulatory authorities. because of their similarity to humans in regard to physiology and anatomy, we are characterizing gottingen minipigs as a model to aid the development of radiation countermeasures. irradiated minipigs exhibit immunosuppression, severe thrombocytopenia, vascular leakage, and acute inflammation. these complications render serial acqu ...201121333166
microsurgical cerebral aneurysm training porcine model.the authors developed a simple reproducible technique for aneurysm creation and adapted it to mimic intracranial dissection conditions using glue application as a pseudo-arachnoid type layer. ten 1-2-month-old healthy domestic swine were employed under general anesthesia. a novel technique for bifurcation aneurysm creation was developed using two arteries and a vein. after aneurysm creation, diluted sulfuric acid was applied on the dome with a micropipette to increase aneurysm fragility in selec ...201121339668
epigenetic reprogramming in the porcine germ line.epigenetic reprogramming is critical for genome regulation during germ line development. genome-wide demethylation in mouse primordial germ cells (pgc) is a unique reprogramming event essential for erasing epigenetic memory and preventing the transmission of epimutations to the next generation. in addition to dna demethylation, pgc are subject to a major reprogramming of histone marks, and many of these changes are concurrent with a cell cycle arrest in the g2 phase. there is limited information ...201121352525
sodium nitroprusside enhanced cardiopulmonary resuscitation improves survival with good neurological function in a porcine model of prolonged cardiac arrest.to assess the effectiveness of sodium nitroprusside (snp)-"enhanced" cardiopulmonary resuscitation (snpecpr) on 24-hr survival rates compared to standard cpr in animals after cardiac arrest. snpecpr consists of large intravenous snp bolus doses during cpr enhanced by active compression-decompression cpr, an inspiratory impedance threshold device (itd), and abdominal binding (ab). the combination of active compression-decompression cpr+itd+ab without snp will be called "enhanced" or ecpr.201121358401
nonlinear microscopy, infrared, and raman microspectroscopy for brain tumor analysis.contemporary brain tumor research focuses on two challenges: first, tumor typing and grading by analyzing excised tissue is of utmost importance for choosing a therapy. second, for prognostication the tumor has to be removed as completely as possible. nowadays, histopathology of excised tissue using haematoxylin-eosine staining is the gold standard for the definitive diagnosis of surgical pathology specimens. however, it is neither applicable in vivo, nor does it allow for precise tumor typing i ...201121361676
the response of cranial biomechanical finite element models to variations in mesh density.finite element (fe) models provide discrete solutions to continuous problems. therefore, to arrive at the correct solution, it is vital to ensure that fe models contain a sufficient number of elements to fully resolve all the detail encountered in a continuum structure. mesh convergence testing is the process of comparing successively finer meshes to identify the point of diminishing returns; where increasing resolution has marginal effects on results and further detail would become costly and u ...201121370496
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