Publications

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jaburetox: update on a urease-derived peptide.urease from canavalia ensiformis seeds was the first enzyme ever to be crystallized, in 1926. these proteins, found in plants, bacteria and fungi, present different biological properties including catalytic hydrolysis of urea, and also enzyme-independent activities, such as induction of exocytosis, pro-inflammatory effects, neurotoxicity, antifungal and insecticidal properties. urease is toxic to insects and fungi per se but part of this toxicity relies on an internal peptide (~11 kda), which is ...201728638403
bacteria-mediated hypoxia functions as a signal for mosquito development.mosquitoes host communities of microbes in their digestive tract that consist primarily of bacteria. we previously reported that several mosquito species, including aedes aegypti, do not develop beyond the first instar when fed a nutritionally complete diet in the absence of a gut microbiota. in contrast, several species of bacteria, including escherichia coli, rescue development of axenic larvae into adults. the molecular mechanisms underlying bacteria-dependent growth are unknown. here, we des ...201728630299
new insights into hcv replication in original cells from aedes mosquitoes.the existing literature about hcv association with, and replication in mosquitoes is extremely poor. to fill this gap, we performed cellular investigations aimed at exploring (i) the capacity of hcv e1e2 glycoproteins to bind on aedes mosquito cells and (ii) the ability of hcv serum particles (hcvsp) to replicate in these cell lines.201728830495
larvicidal potency of marine actinobacteria isolated from mangrove environment against aedes aegypti and anopheles stephensi.the marine soil samples were collected from different locations of parangipettai mangrove ecosystem, vellar estuary, southeast coast of india. totally 30 different marine actinobacteria were isolated by serial dilution plate technique on starch casein agar medium. the isolated actinobacteria were investigated for their larvicidal activity against aedes aegypti and anopheles stephensi mosquitoes. streptomyces fungicidicus, s. griseus, s. albus, s. alboflavus and s. rochei were identified as poten ...201728615847
efficacy of the vegetative cells of lysinibacillus sphaericus for biological control of insecticide-resistant aedes aegypti.the control of aedes aegypti is usually based on chemical insecticides, but the overuse of these compounds has led to increased resistance. the binary toxin produced by lysinibacillus sphaericus in the final stages of sporulation is used for mosquito control due to its specificity against the culicid larvae; however, it has been proved that ae. aegypti is refractory for this toxin. currently, there is no evidence of the use of l. sphaericus vegetative cells for mosquito biocontrol. therefore, in ...201728490350
mycobacterium ulcerans low infectious dose and mechanical transmission support insect bites and puncturing injuries in the spread of buruli ulcer.addressing the transmission enigma of the neglected disease buruli ulcer (bu) is a world health organization priority. in australia, we have observed an association between mosquitoes harboring the causative agent, mycobacterium ulcerans, and bu. here we tested a contaminated skin model of bu transmission by dipping the tails from healthy mice in cultures of the causative agent, mycobacterium ulcerans. tails were exposed to mosquito (aedes notoscriptus and aedes aegypti) blood feeding or punctur ...201728410412
vector competence of aedes albopictus (skuse) and aedes aegypti (linnaeus) for plasmodium gallinaceum infection and transmission.avian malaria caused by plasmodium gallinaceum is an important mosquito-borne disease. eradication of this disease remains problematic since its competent vectors are diverse and widely distributed across the globe. several mosquito species were implicated as competent vectors for this parasite. however, studies on vector competence for p. gallinaceum remain limited. in this study, vector competence in the two most predominant mosquito vectors in tropical countries, aedes albopictus and ae. aegy ...201728579025
synergistic efficacy of aedes aegypti antimicrobial peptide cecropin a2 and tetracycline against pseudomonas aeruginosa.the increasing prevalence of antibiotic resistance has created an urgent need for alternative drugs with new mechanisms of action. antimicrobial peptides (amps) are promising candidates that could address the spread of multidrug-resistant bacteria, either alone or in combination with conventional antibiotics. we studied the antimicrobial efficacy and bactericidal mechanism of cecropin a2, a 36-residue α-helical cationic peptide derived from aedes aegypti cecropin a, focusing on the common pathog ...201728483966
aedes aegypti piwi4 is a noncanonical piwi protein involved in antiviral responses.the small interfering rna (sirna) pathway is a major antiviral response in mosquitoes; however, another rna interference pathway, the piwi-interacting rna (pirna) pathway, has been suggested to be antiviral in mosquitoes. piwi4 has been reported to be a key mediator of this response in mosquitoes, but it is not involved in the production of virus-specific pirnas. here, we show that piwi4 associates with members of the antiviral exogenous sirna pathway (ago2 and dcr2), as well as with proteins of ...201728497119
characterization of tolypocladium cylindrosporum (hypocreales: ophiocordycipitaceae) and its impact against aedes aegypti and aedes albopictus eggs at low temperature.we examined the growth characteristics of tolypocladium cylindrosporum ibt 41712 and its potential to infect eggs of aedes aegypti and ae. albopictus at a low temperature (15°c). when grown on sabouraud dextrose agar supplemented with yeast extract, the ibt 41712 formed white colonies turning to a slightly darker, off-white color when mature. the mycelia bore swollen conidiophores producing smooth-walled, oblong to cylindrical conidia with varying sizes, ranging from 1.5 to 3.5 μm long. to deter ...201728854112
the role of the environment in the evolution of tolerance and resistance to a pathogen.defense against parasites can be divided into resistance, which limits parasite burden, and tolerance, which reduces pathogenesis at a given parasite burden. distinguishing between the two and understanding which defense is favored by evolution in different ecological settings are important, as they lead to fundamentally different evolutionary trajectories of host-parasite interactions. we let the mosquito aedes aegypti evolve under different food levels and with either no parasite, a constant p ...201728829641
specificity and putative mode of action of a mosquito larvicidal toxin from the bacterium xenorhabdus innexi.reduction of mosquito-borne diseases relies, in part, on the use of synthetic pesticides to control pest mosquitoes. this reliance has led to genetic resistance, environmental contamination and the nondiscriminatory elimination of both pest and non-pest species. to expand our options for control, we screened entomopathogenic bacteria for potential larvicidal activity. a lipopeptide from the bacterium, xenorhabdus innexi, was discovered that displayed potent larvicidal activity. the lc50s of the ...201728712711
potential risk of re-emergence of urban transmission of yellow fever virus in brazil facilitated by competent aedes populations.yellow fever virus (yfv) causing a deadly viral disease is transmitted by the bite of infected mosquitoes. in brazil, yfv is restricted to a forest cycle maintained between non-human primates and forest-canopy mosquitoes, where humans can be tangentially infected. since late 2016, a growing number of human cases have been reported in southeastern brazil at the gates of the most populated areas of south america, the atlantic coast, with rio de janeiro state hosting nearly 16 million people. we sh ...201728687779
taking innovative vector control interventions in urban latin america to scale: lessons learnt from multi-country implementation research.prior to the current public health emergency following the emergence of chikungunya and zika virus disease in the americas during 2014 and 2015, multi-country research investigated between 2011 and 2013 the efficacy of novel aedes aegypti intervention packages through cluster randomised controlled trials in four latin-american cities: fortaleza (brazil); girardot (colombia), acapulco (mexico) and salto (uruguay). results from the trials led to a scaling up effort of the interventions at city lev ...201728829235
modeling the transmission and control of zika in brazil.zika virus, a reemerging mosquito-borne flavivirus, started spread across central and southern america and more recently to north america. the most serious impacted country is brazil. based on the transmission mechanism of the virus and assessment of the limited data on the reported suspected cases, we establish a dynamical model which allows us to estimate the basic reproduction number r 0 = 2.5020. the wild spreading of the virus make it a great challenge to public health to control and preven ...201728798323
differential outcomes of zika virus infection in aedes aegypti orally challenged with infectious blood meals and infectious protein meals.infection of mosquitoes is an essential step for the transmission of mosquito-borne arboviruses in nature. engorgement of infectious blood meals from viremic infected vertebrate hosts allows the entry of viruses and initiates infection of midgut epithelial cells. historically, the infection process of arboviruses in mosquitoes has been studied through the engorgement of mosquitoes from viremic laboratory animals or from artificial feeders containing blood mixed with viruses harvested from cell c ...201728796799
zika virus replication in the mosquito culex quinquefasciatus in brazil.zika virus (zikv) is a flavivirus that has recently been associated with an increased incidence of neonatal microcephaly and other neurological disorders. the virus is primarily transmitted by mosquito bite, although other routes of infection have been implicated in some cases. the aedes aegypti mosquito is considered to be the main vector to humans worldwide; however, there is evidence that other mosquito species, including culex quinquefasciatus, transmit the virus. to test the potential of cx ...201728790458
sexual and vertical transmission of zika virus in anti-interferon receptor-treated rag1-deficient mice.although zika virus (zikv) is primarily transmitted to humans by the aedes aegypti mosquito, human-to-human transmission has also been observed from males-to-females as well as mother-to-offspring. in the current study, we studied both sexual transmission (stx) and vertical transmission (vtx) of zikv using anti-ifnar1-treatment of rag1 (-/-) (air) mice. these mice have suppressed type i ifn responses and lack adaptive immune responses, leading to a prolonged infection prior to clinical disease. ...201728775298
culex quinquefasciatus from areas with the highest incidence of microcephaly associated with zika virus infections in the northeast region of brazil are refractory to the virus.zika virus (zikv) is widely distributed in brazil and the northeast region (ne) is the most affected zone, showing the highest incidence of microcephaly associated with zikv congenital infections worldwide. we report attempts to infect three populations of culex quinquefasciatus from severely affected sites in the ne and southeast region (se) of brazil with three strains of zikv isolated from these localities. an aedes aegypti population from the se was used as a positive control. all tested cx. ...201728767975
linking only aedes aegypti with zika virus has world-wide public health implications. 201728736548
zika virus transmission to mouse ear by mosquito bite: a laboratory model that replicates the natural transmission process.zika disease has transformed into a serious global health problem due to the rapid spread of the arbovirus and alarming severity including congenital complications, microcephaly and guillain-barré syndrome. zika virus (zikv) is primarily transmitted to humans through the bite of an infective mosquito, with aedes aegypti being the main vector.201728728607
asymptomatic transmission and the dynamics of zika infection.following the 2013-14 outbreak in french polynesia, the zika virus (zikv) epidemic spread widely to many countries where aedes aegypti as the main transmitting vector is endemic. the lack of a second wave of zikv infection in most affected regions may suggest that a sufficiently high level of herd immunity was reached during the first wave. we developed an agent-based transmission model to investigate the role of asymptomatic infection on the likelihood of observing a second wave, while accounti ...201728724972
american aedes vexans mosquitoes are competent vectors of zika virus.abstractstarting in 2013-2014, the americas have experienced a massive outbreak of zika virus (zikv) which has now reached at least 49 countries. although most cases have occurred in south america and the caribbean, imported and autochthonous cases have occurred in the united states. aedes aegypti and aedes albopictus mosquitoes are known vectors of zikv. little is known about the potential for temperate aedes mosquitoes to transmit zikv. aedes vexans has a worldwide distribution, is highly abun ...201728719283
early evidence for zika virus circulation among aedes aegypti mosquitoes, rio de janeiro, brazil.during 2014-2016, we conducted mosquito-based zika virus surveillance in rio de janeiro, brazil. results suggest that zika virus was probably introduced into the area during may-november 2013 via multiple in-country sources. furthermore, our results strengthen the hypothesis that zika virus in the americas originated in brazil during october 2012-may 2013.201728628464
a single substitution changes zika virus infectivity in mosquitoes.zika virus (zikv) has caused outbreaks in the pacific and the americas. the mechanism underlying the recent zikv epidemic remains obscure. a recent study reveals that an amino acid substitution is associated with increased infectivity of zikv in the aedes aegypti mosquito.201728610876
zika vector transmission risk in temperate australia: a vector competence study.zika virus is an emerging pathogen of global importance. it has been responsible for recent outbreaks in the americas and in the pacific region. this study assessed five different mosquito species from the temperate climatic zone in australia and included aedes albopictus as a potentially invasive species.201728599659
emerging infectious disease agents and blood safety in australia: spotlight on zika virus.emerging infectious diseases (eids) are infectious diseases whose incidence has increased in humans in the past 20 years or could increase in the near future. eid agents may represent a threat to blood safety if they infect humans, cause a clinically significant illness, include an asymptomatic blood phase in the course of infection, and are transmissible by transfusion. eid agents are typically not well characterised, but there is a consensus that we can expect ongoing outbreaks. strategies to ...201728566073
genomic epidemiology reveals multiple introductions of zika virus into the united states.zika virus (zikv) is causing an unprecedented epidemic linked to severe congenital abnormalities. in july 2016, mosquito-borne zikv transmission was reported in the continental united states; since then, hundreds of locally acquired infections have been reported in florida. to gain insights into the timing, source, and likely route(s) of zikv introduction, we tracked the virus from its first detection in florida by sequencing zikv genomes from infected patients and aedes aegypti mosquitoes. we s ...201728538723
de novo generation and characterization of new zika virus isolate using sequence data from a microcephaly case.zika virus (zikv) has recently emerged and is the etiological agent of congenital zika syndrome (czs), a spectrum of congenital abnormalities arising from neural tissue infections in utero. herein, we describe the de novo generation of a new zikv isolate, zikvnatal, using a modified circular polymerase extension reaction protocol and sequence data obtained from a zikv-infected fetus with microcephaly. zikvnatal thus has no laboratory passage history and is unequivocally associated with czs. zikv ...201728529976
outbreak of zika virus infection in singapore: an epidemiological, entomological, virological, and clinical analysis.an outbreak of zika virus infection was detected in singapore in august, 2016. we report the first comprehensive analysis of a national response to an outbreak of zika virus infection in asia.201728527892
eco-friendly and cost-effective ag nanocrystals fabricated using the leaf extract of habenaria plantaginea: toxicity on six mosquito vectors and four non-target species.recently, the biofabrication of metal nanoparticles has gained wide interest owing to its inherent features such as swift, simplicity, eco-friendliness, and cheaper costs. different green-reducing agents led to the production of nanoparticles with varying toxicity on insects. in the current study, silver nanoparticles (agnps) were successfully synthesized using habenaria plantaginea leaf extract. ag nanoparticles were studied by uv-vis spectroscopy (uv-vis), x-ray diffraction (xrd), fourier tran ...201728523613
evolutionary enhancement of zika virus infectivity in aedes aegypti mosquitoes.zika virus (zikv) remained obscure until the recent explosive outbreaks in french polynesia (2013-2014) and south america (2015-2016). phylogenetic studies have shown that zikv has evolved into african and asian lineages. the asian lineage of zikv was responsible for the recent epidemics in the americas. however, the underlying mechanisms through which zikv rapidly and explosively spread from asia to the americas are unclear. non-structural protein 1 (ns1) facilitates flavivirus acquisition by m ...201728514450
a location-specific spreadsheet for estimating zika risk and timing for zika vector surveillance, using us military facilities as an example.local zika virus transmission in the united states involving one or both of the known vector species, aedes aegypti and ae. albopictus, is of major concern. to assist efforts to anticipate the risks of transmission, we developed an excel spreadsheet tool that uses vector and virus temperature thresholds, remotely sensed maximum temperature, and habitat suitability models to answer the questions: "is zika transmission likely here?" and "when should we conduct vector surveillance?" an example spre ...201728511272
transmission incompetence of culex quinquefasciatus and culex pipiens pipiens from north america for zika virus.abstractin late 2014, zika virus (zikv; flaviviridae, flavivirus) emerged as a significant arboviral disease threat in the western hemisphere. aedes aegypti and aedes albopictus have been considered the principal vectors of zikv in the new world due to viral isolation frequency and vector competence assessments. limited reports of culex transmission potential have highlighted the need for additional vector competence assessments of north american culex species. accordingly, north american culex ...201728500817
potential of a northern population of aedes vexans (diptera: culicidae) to transmit zika virus.zika virus is an emerging arbovirus of humans in the western hemisphere. with its potential spread into new geographical areas, it is important to define the vector competence of native mosquito species. we tested the vector competency of aedes vexans (meigen) from the lake agassiz plain of northwestern minnesota and northeastern north dakota. aedes aegypti (l.) was used as a positive control for comparison. mosquitoes were fed blood containing zika virus and 2 wk later were tested for viral inf ...201728499036
a zika virus from america is more efficiently transmitted than an asian virus by aedes aegypti mosquitoes from asia.zika is a mosquito-borne disease associated with neurological disorders that causes an on-going pandemic. the first outbreak was recorded in micronesia in 2007, then in french polynesia in 2014 from which it spread to south america in 2015 and ignited a widespread epidemic. interestingly, zika outbreaks in asia remained of moderate intensity although the virus is circulating. to understand these epidemiological variations, we investigated the entomological determinants of zikv transmission in as ...201728450714
vector competence and transovarial transmission of two aedes aegypti strains to zika virus.zika virus (zikv) has become a serious threat to global health since the outbreak in brazil in 2015. additional chinese cases have continuously been reported since the first case of laboratory-confirmed zikv infection in china on 6 february 2016. aedes aegypti is the most important vector for zikv. this study shows that two strains from china exhibit high levels of midgut infection and highly disseminated infection of salivary glands and ovaries. both strains can transmit zikv to infant mice bit ...201728442754
effects of zika virus strain and aedes mosquito species on vector competence.in the western hemisphere, zika virus is thought to be transmitted primarily by aedes aegypti mosquitoes. to determine the extent to which ae. albopictus mosquitoes from the united states are capable of transmitting zika virus and the influence of virus dose, virus strain, and mosquito species on vector competence, we evaluated multiple doses of representative zika virus strains in ae. aegypti and ae. albopictus mosquitoes. virus preparation (fresh vs. frozen) significantly affected virus infect ...201728430564
competence of aedes aegypti, ae. albopictus, and culex quinquefasciatus mosquitoes as zika virus vectors, china.in china, the prevention and control of zika virus disease has been a public health threat since the first imported case was reported in february 2016. to determine the vector competence of potential vector mosquito species, we experimentally infected aedes aegypti, ae. albopictus, and culex quinquefasciatus mosquitoes and determined infection rates, dissemination rates, and transmission rates. we found the highest vector competence for the imported zika virus in ae. aegypti mosquitoes, some sus ...201728430562
evidence of zika virus rna fragments in aedes albopictus (diptera: culicidae) field-collected eggs from camaçari, bahia, brazil.a major mosquito-borne viral disease outbreak caused by zika virus (zikv) occurred in bahia, brazil, in 2015, largely due to transmission by the mosquito, aedes aegypti (l.). detecting zikv in field samples of ae. aegypti has proven problematic in some locations, suggesting other mosquito species might be contributing to the spread of zikv. in this study, several (five) adult aedes albopictus (skuse) mosquitoes that emerged from a 2015 field collection of eggs from camaçari, bahia, brazil, were ...201728419254
chemosensory responses to the repellent nepeta essential oil and its major component nepetalactone by aedes aegypti (diptera: culicidae), a vector of zika virus.nepeta essential oil (neo; catnip) and its major component, nepetalactone, have long been known to repel insects including mosquitoes. however, the neural mechanisms through which these repellents are detected by mosquitoes, including the yellow fever mosquito aedes aegypti (l.), an important vector of zika virus, were poorly understood. here we show that neo volatiles activate olfactory receptor neurons within the basiconic sensilla on the maxillary palps of female ae. aegypti. a gustatory rece ...201728407077
quantitative proteomic analysis of mosquito c6/36 cells reveals host proteins involved in zika virus infection.zika virus (zikv) is an emerging arbovirus belonging to the genus flavivirus of the family flaviviridae during replication processes, flavivirus manipulates host cell systems to facilitate its replication, while the host cells activate antiviral responses. identification of host proteins involved in the flavivirus replication process may lead to the discovery of antiviral targets. the mosquitoes aedes aegypti and aedes albopictus are epidemiologically important vectors for zikv, and effective re ...201728404849
modelling dengue fever risk in the state of yucatan, mexico using regional-scale satellite-derived sea surface temperature.accurately predicting vector-borne diseases, such as dengue fever, is essential for communities worldwide. changes in environmental parameters such as precipitation, air temperature, and humidity are known to influence dengue fever dynamics. furthermore, previous studies have shown how oceanographic variables, such as el niño southern oscillation (enso)-related sea surface temperature from the pacific ocean, influences dengue fever in the americas. however, literature is lacking on the use of re ...201728450208
detecting the impact of temperature on transmission of zika, dengue, and chikungunya using mechanistic models.recent epidemics of zika, dengue, and chikungunya have heightened the need to understand the seasonal and geographic range of transmission by aedes aegypti and ae. albopictus mosquitoes. we use mechanistic transmission models to derive predictions for how the probability and magnitude of transmission for zika, chikungunya, and dengue change with mean temperature, and we show that these predictions are well matched by human case data. across all three viruses, models and human case data both show ...201728448507
juvenile hormone and its receptor methoprene-tolerant promote ribosomal biogenesis and vitellogenesis in the aedes aegypti mosquito.juvenile hormone (jh) controls many biological activities in insects, including development, metamorphosis, and reproduction. in the aedes aegypti mosquito, a vector of dengue, yellow fever, chikungunya, and zika viruses, the metabolic tissue (the fat body, which is an analogue of the vertebrate liver) produces yolk proteins for developing oocytes. jh is important for the fat body to acquire competence for yolk protein production. however, the molecular mechanisms of how jh promotes mosquito rep ...201728446607
species abundance and temporal variation of arbovirus vectors in brownsville, texas.the recent outbreaks of the dengue fever and west nile viruses and the looming threats of the zika and chikungunya viruses highlight the importance of establishing effective, proactive arboviral surveillance in communities at high risk of transmission, such as those on the texas-mexico border. currently, there are no approved human vaccines available for these mosquito-borne diseases, so entomological control and case management are the only known methods for decreasing disease incidence. the pr ...201728444003
dengue virus replicates and accumulates in aedes aegypti salivary glands.dengue virus (denv) is an rna virus transmitted among humans by mosquito vectors, mainly aedes aegypti. denv transmission requires viral dissemination from the mosquito midgut to the salivary glands. during this process the virus undergoes several population bottlenecks, which are stochastic reductions in population size that restrict intra-host viral genetic diversity and limit the efficiency of natural selection. despite the implications for virus transmission and evolution, denv replication i ...201728431281
yellow fever from angola and congo: a storm gathers.in common with zika, chikungunya and dengue, yellow fever (yf) is an arthropod-borne flavivirus. it is transmitted between humans and from monkeys by mosquitoes of the aedes aegypti (its principal vector), haemogogus and albopictus varieties. three cycles of transmission may occur: urban; sylvatic; and intermediate. recently, sub-saharan africa has seen the resurgence of this neglected disease. the current yf outbreak in angola began in december 2015 in the capital luanda and by october 2016 the ...201728424031
detection of a new pyrethroid resistance mutation (v410l) in the sodium channel of aedes aegypti: a potential challenge for mosquito control.the yellow fever mosquito, aedes aegypti, particularly in neotropical regions, is the principal vector of dengue, yellow fever, zika and chikungunya viruses. pyrethroids remain one of the most used insecticides to control aedes mosquitoes, despite the development of pyrethroid resistance in many mosquito populations worldwide. here, we report a brazilian strain of a. aegypti with high levels (approximately 100-60,000 fold) of resistance to both type i and type ii pyrethroids. we detected two mut ...201728422157
deploying dengue-suppressing wolbachia : robust models predict slow but effective spatial spread in aedes aegypti.a novel strategy for controlling the spread of arboviral diseases such as dengue, zika and chikungunya is to transform mosquito populations with virus-suppressing wolbachia. in general, wolbachia transinfected into mosquitoes induce fitness costs through lower viability or fecundity. these maternally inherited bacteria also produce a frequency-dependent advantage for infected females by inducing cytoplasmic incompatibility (ci), which kills the embryos produced by uninfected females mated to inf ...201728411063
severe dengue due to secondary hemophagocytic lymphohistiocytosis: a case study.dengue, transmitted by the mosquito aedes aegypti affects millions of people worldwide every year. dengue induced hemophagocytic lymphohistiocytosis (hlh) is a serious condition and may prove fatal if not detected early and treated appropriately. diagnosis of hlh is challenging and usually missed as clinical and laboratory findings are nonspecific. moreover, the pathophysiology of the systemic inflammatory response syndrome and/or sepsis is remarkably similar to hlh. secondary hlh following infe ...201728409119
vector competence and innate immune responses to dengue virus infection in selected laboratory and field-collected stegomyia aegypti (= aedes aegypti).control of dengue virus (denv) transmission, primarily based on strategies to reduce populations of the principle vector stegomya aegypti (= aedes aegypti) (diptera: culicidae), is difficult to sustain over time. other potential strategies aim to manipulate characteristics such as vector competence (vc), the innate capacity of the vector to transmit the virus. previous studies have identified genetic factors, including differential expression of apoptosis-related genes, associated with the refra ...201728407282
advances in vector control science: rear-and-release strategies show promise… but don't forget the basics.both chikungunya and zika viruses have recently swept from africa across the pacific to the americas, causing major outbreaks of disease in humans. in the meantime, dengue epidemics continue throughout the tropics. traditional vector control programs based on strategies from 1950s and 1960s have been relatively ineffective in combating recent epidemics. in response, new methods involving the rearing and releasing of large numbers of mosquitoes to eliminate or modify local aedes populations are b ...201728403439
aedes aegypti (diptera: culicidae) abundance model improved with relative humidity and precipitation-driven egg hatching.we propose an improved aedes aegypti (l.) abundance model that takes into account the effect of relative humidity (rh) on adult survival, as well as rainfall-triggered egg hatching. the model uses temperature-dependent development rates described in the literature as well as documented estimates for mosquito survival in environments with high rh, and for egg desiccation. we show that combining the two additional components leads to better agreement with surveillance trap data and with dengue inc ...201728402546
repellency of 29 synthetic and natural commercial topical insect repellents against aedes aegypti (diptera: culicidae) in central mexico.in mexico, the use of repellents to prevent insects from landing and biting is a common practice. however, variation in the efficiency of natural and synthetic repellents has been observed. in this study, we evaluated the repellency and protection time of 16 synthetic and 13 natural-based commercial products against aedes aegypti (l.) from an endemic dengue area (jojutla, morelos) in central mexico. the "arm exposure" cage test was used to assess the efficacy of the repellents. tests were conduc ...201728402436
why is aedes aegypti linnaeus so successful as a species?diseases transmitted by mosquitoes impose enormous burden towards human morbidity and mortality. over the last three decades, brazil has suffered from severe dengue epidemics. in september 2014, this situation is further complicated by the introduction of two other viruses, zika and chikungunya, placing brazil in a triple epidemic. in this article, we discuss the biology of aedes aegypti linnaeus, and the principal initiatives currently used to control mosquito populations and the diseases they ...201728401481
full-genome dengue virus sequencing in mosquito saliva shows lack of convergent positive selection during transmission byaedes aegypti.like other pathogens with high mutation and replication rates, within-host dengue virus (denv) populations evolve during infection of their main mosquito vector,aedes aegypti. within-host denv evolution during transmission provides opportunities for adaptation and emergence of novel virus variants. recent studies of denv genetic diversity failed to detect convergent evolution of adaptive mutations in mosquito tissues such as midgut and salivary glands, suggesting that convergent positive selecti ...201729497564
laboratory evaluation of a novel lethal ovitrap for control of aedes aegypti.aedes aegypti (l.) (diptera: culicidae) are known to flourish in a variety of natural and residential habitats and are competent vectors of at least 22 different arboviruses, including dengue, chikungunya, and zika. their global distribution, anthropophilic nature, and vector competency make them species of interest for control. a novel durable dual-action lethal ovitrap (ddalo) with combined larviciding and adulticiding effects, as well as a slow-release polymer (isobutyl methacrylate), was des ...201728968892
comparative efficacy of commercial mosquito coils against aedes aegypti (diptera: culicidae) in malaysia: a nationwide report.this study was conducted using the glass chamber method to determine the susceptibility status of the dengue vector, aedes aegypti (l.) from 11 states in malaysia to commercial mosquito coils containing four different active ingredients, namely metofluthrin, d-allethrin, d-trans allethrin, and prallethrin. aedes aegypti exhibited various knockdown rates, ranging from 14.44% to 100.00%, 0.00% to 61.67%, 0.00% to 90.00%, and 0.00% to 13.33% for metofluthrin, d-allethrin, d-trans allethrin, and pra ...201729048583
evaluation of embryotoxic and embryostatic effects of the aqueous extract of rhizophora mangle and tannic acid on eggs and larvae of aedes aegypti.rhizophora mangle is an abundant plant in mangroves and tannic acid is a polyphenol produced by the secondary metabolism of plants. the aim of the study was to evaluate the embryotoxic and embriostatic effects of the aqueous extract of r. mangle and synthetic tannic acid on eggs and larvae of aedes aegypti. a. aegypti eggs were exposed in duplicate at concentrations of 250, 500, 750 and 1000 µg/ml of extract and tannic acid for a period of 14 days. mineral water was used as a negative control. t ...201729044325
perturbed cholesterol and vesicular trafficking associated with dengue blocking in wolbachia-infected aedes aegypti cells.wolbachia are intracellular maternally inherited bacteria that can spread through insect populations and block virus transmission by mosquitoes, providing an important approach to dengue control. to better understand the mechanisms of virus inhibition, we here perform proteomic quantification of the effects of wolbachia in aedes aegypti mosquito cells and midgut. perturbations are observed in vesicular trafficking, lipid metabolism and in the endoplasmic reticulum that could impact viral entry a ...201728904344
minimum infectious dose for chikungunya virus in aedes aegypti and ae. albopictus mosquitoes.understanding the ability of the chikungunya virus (chikv) to be transmitted by aedes vectors in the americas is critical for assessing epidemiological risk. one element that must be considered is the minimum infectious dose of virus that can lead to transmission following the extrinsic incubation period. this study aimed to determine the minimum infection rate for the two aedes species studied. the results revealed that doses as low as 3.9 log10 plaque-forming units per ml (pfu/ml) of an asian ...201728902278
first detection of dengue and chikungunya viruses in natural populations of aedes aegypti in martinique during the 2013 - 2015 concomitant outbreak.dengue and chikungunya viruses are transmitted by aedes mosquitoes. in martinique, an island of the french west indies, aedes aegypti is the suspected vector of both arboviruses; there is no aedes albopictus on the island. during the concomitant outbreak of 2013 - 2015, the authors collected wild a. aegypti populations, and for the first time, detected dengue and chikungunya viruses in field-collected females. this paper demonstrates the mosquito's role in transmission of both dengue and chikung ...201728902276
highly effective broad spectrum chimeric larvicide that targets vector mosquitoes using a lipophilic protein.two mosquitocidal bacteria, bacillus thuringiensis subsp. israelensis (bti) and lysinibacillus sphaericus (ls) are the active ingredients of commercial larvicides used widely to control vector mosquitoes. bti's efficacy is due to synergistic interactions among four proteins, cry4aa, cry4ba, cry11aa, and cyt1aa, whereas ls's activity is caused by bin, a heterodimer consisting of bina, the toxin, and binb, a midgut-binding protein. cyt1aa is lipophilic and synergizes bti cry proteins by increasing ...201728900215
efficacy of blood sources and artificial blood feeding methods in rearing of aedes aegypti (diptera: culicidae) for sterile insect technique and incompatible insect technique approaches in sri lanka.selection of the artificial membrane feeding technique and blood meal source has been recognized as key considerations in mass rearing of vectors.201728894749
a natural hybrid of a bacillus thuringiensis cry2a toxin implicates domain i in specificity determination.a pcr-rflp method was used to identify cry2a toxin genes in a collection of 300 strains of bacillus thuringiensis. from 81 genes identified, the vast majority appeared to be cry2aa or cry2ab, however three showed a different pattern and were subsequently cloned and sequenced. the gene cloned from strain hd395 was named cry2ba2. since the proteins encoded by the genes cloned from ls5115-3 and ds415 shared >95% sequence identity with existing toxins their genes were named cry2aa17 and cry2ab29 res ...201728888766
isolation of a larvicidal compound from piper solmsianum c.dc. (piperaceae).the aedes aegypti mosquito is one of the major vectors of arboviruses. these diseases have re-emerged and the insecticides used nowadays are toxic to mammals and environment and have only been effective in the short-term. in this context, natural products are an alternative. the genus piper has many active compounds against arthropods, including neolignans. the present study evaluated the larvicidal potential of the n-hexanic extract of piper solmsianum and eupomatenoid-6, identified by gc-ms an ...201728882070
association between suspected zika virus disease during pregnancy and giving birth to a newborn with congenital microcephaly: a matched case-control study.in early 2015, an outbreak of an acute exanthematous illness with dengue-like symptoms occurred in northeastern brazil. by the end of the same year, an unexpected increase in the number of cases of microcephaly was observed in the region. the microcephaly outbreak cause was unknown and rumors pointing to various potential causes arose. since we were unaware at the time if this scenario would attract the interest of the broader scientific community, due to the neglected regions associated and as ...201728877754
microrna-277 targets insulin-like peptides 7 and 8 to control lipid metabolism and reproduction in aedes aegypti mosquitoes.hematophagous female mosquitoes transmit numerous devastating human diseases, including malaria, dengue fever, zika virus, and others. because of their obligatory requirement of a vertebrate blood meal for reproduction, these mosquitoes need a lot of energy; therefore, understanding the molecular mechanisms linking metabolism and reproduction is of particular importance. lipids are the major energy store providing the fuel required for host seeking and reproduction. they are essential components ...201728874536
updated reported distribution of aedes (stegomyia) aegypti and aedes (stegomyia) albopictus (diptera: culicidae) in the united states, 1995-2016.aedes (stegomyia) aegypti (l.) and aedes (stegomyia) albopictus (skuse) are potential vectors of zika, dengue, and chikungunya viruses in the united states. a zika virus outbreak in florida in the summer of 2016, driven by ae. aegypti and resulting in > 200 locally acquired cases of human illness, underscored the need for up-to-date information on the geographic distribution of ae. aegypti and ae. albopictus in the united states. in early 2016, we conducted a survey and literature review to comp ...201728874014
"we need people to collaborate together against this disease": a qualitative exploration of perceptions of dengue fever control in caregivers' of children under 5 years, in the peruvian amazon.dengue fever presents a significant and growing burden of disease to endemic countries, where children are at particular risk. worldwide, no effective anti-viral treatment has been identified, thus vector control is key for disease prevention, particularly in peru where no vaccine is currently available. this qualitative study aimed to explore the perceptions of dengue control in caregivers' of children under 5 years in peru, to help direct future mosquito control programmes and strategy.201728873408
fat body organ culture system in aedes aegypti, a vector of zika virus.the insect fat body plays a central role in insect metabolism and nutrient storage, mirroring functions of the liver and fat tissue in vertebrates. insect fat body tissue is usually distributed throughout the insect body. however, it is often concentrated in the abdomen and attached to the abdominal body wall. the mosquito fat body is the sole source of yolk proteins, which are critical for egg production. therefore, the in vitro culture of mosquito fat body tissues represents an important syste ...201728872112
rapid evolution of ovarian-biased genes in the yellow fever mosquito (aedes aegypti).males and females exhibit highly dimorphic phenotypes, particularly in their gonads, which is believed to be driven largely by differential gene expression. typically, the protein sequences of genes upregulated in males, or male-biased genes, evolve rapidly as compared to female-biased and unbiased genes. to date, the specific study of gonad-biased genes remains uncommon in metazoans. here, we identified and studied a total of 2927, 2013, and 4449 coding sequences (cds) with ovary-biased, testis ...201728630112
determinants of outbreaks of emerging infectious diseases : the case of chikungunya in indian ocean, 2004-2007.emerging outbreaks of arboviroses are most often tied to the convergence of a set of factors which have physical and environmental, genetic and biological, ecological, social, political and economic origins. thirteen out of these factors are known as main determinants of emerging infectious disease outbreaks. this paper assesses the role of these factors from exploring the chikungunya outbreak which occurred in indian ocean in 2004-2007, as case study. chikungunya is an alphavirus borne and tran ...201728627853
the genetic diversity and population structure of domestic aedes aegypti (diptera: culicidae) in yunnan province, southwestern china.there was no record of aedes aegypti in yunnan province, china, until 2002, but this species is now continuously found in nine cities (or counties). until now, little was known about the genetic diversity and population structure of this invasive species. thus, a detailed understanding of the invasion strategies, colonisation and dispersal of this mosquito from a population genetics perspective is urgently needed for controlling and eliminating this disease vector.201728610594
deltamethrin resistance in aedes aegypti results in treatment failure in merida, mexico.the operational impact of deltamethrin resistance on the efficacy of indoor insecticide applications to control aedes aegypti was evaluated in merida, mexico. a randomized controlled trial quantified the efficacy of indoor residual spraying (irs) against adult ae. aegypti in houses treated with either deltamethrin (to which local ae. aegypti expressed a high degree of resistance) or bendiocarb (to which local ae. aegypti were fully susceptible) as compared to untreated control houses. all adult ...201728604781
qsar models for predicting the toxicity of piperidine derivatives against aedes aegypti.qsar models are proposed for predicting the toxicity of 33 piperidine derivatives against aedes aegypti. from 2d topological descriptors, calculated with the padel software, ordinary least squares multilinear regression (ols-mlr) treatment from the qsarins software and machine learning and related approaches including linear and radial support vector machine (svm), projection pursuit regression (ppr), radial basis function neural network (rbfnn), general regression neural network (grnn) and k-ne ...201728604113
[new strategies for the eradication of aedes aegypti: what challenges do we face in latin america?]not available.201728603242
comparative efficiency of biogents gravid aedes trap, cdc autocidal gravid ovitrap, and cdc gravid trap in northeastern florida.we conducted a study to compare the effectiveness of the biogents gravid aedes trap (bg-gat) and centers for disease control and prevention (cdc) autocidal gravid ovitrap (ago) with that of the cdc gravid trap (cdc-gt) (as a standard) for their proficiency to collect mosquitoes in an urban residential neighborhood in northeastern florida. aedes aegypti , ae. albopictus, and culex quinquefasciatus were collected from each trap, with the latter species being predominant. significantly more cx. qui ...201728590226
comparison of mosquito abundance from biogents sentinel 2.0 traps with and without rain covers.biogents sentinel (bgs) traps have rapidly become a standard for adult aedes aegypti surveillance. several investigators have found that trap collections can be damaged easily by heavy dew or rain entering the trap intake port. in addition, water entering the trap may temporarily stop the fan, thus reducing the collection potential of the trap. we evaluated the effectiveness of a rain cover designed to minimize damage to mosquito trap collections from bgs 2.0 traps in a residential backyard in j ...201728590218
diagnostic doses of insecticides for adult aedes aegypti to assess insecticide resistance in cuba.the objective of this study was to determine diagnostic doses (dds) of 5 insecticides for the rockefeller susceptible strain of aedes aegypti , using the centers for disease control and prevention (cdc) bottle bioassay as a tool for monitoring insecticide resistance in the cuban vector control program. the 30-min dd values determined in this study were 13.5 μg/ml, 6.5 μg/ml, 6 μg/ml, 90.0 μg/ml, and 15.0 μg/ml for cypermethrin, deltamethrin, lambda-cyhalothrin, chlorpyrifos, and propoxur, respec ...201728590216
effects of low-powered rf sweep between 0.01-20 ghz on female aedes aegypti mosquitoes: a collective behaviour analysis.there are many products claiming to be an electronic solution towards repelling mosquitoes. several reviews were published in debunking these claims. however, there is a lack of a systematic study on effects of electromagnetic (em) or more specifically, radio frequency (rf) waves against mosquitoes due to the conclusions made in those years. therefore, we attempt to establish a fundamental study on female aedes aegypti (linnaeus) mosquitoes by quantifying the collective behavior of the mosquitoe ...201728582398
corrigendum to "functional and immunohistochemical characterization of cceae3a, a carboxylesterase associated with temephos resistance in the major arbovirus vectors aedes aegypti and ae. albopictus" [insect biochem. mol. biol. 74 (july 2016) 61-67]. 201728578775
detection and perception of generic host volatiles by mosquitoes: responses to co2 constrains host-seeking behaviour.natural selection has favoured specialization in anthropophilic mosquito host choice, yet in the absence of human hosts, females feed on a selected range of vertebrates. for host recognition, we hypothesize that mosquitoes primarily rely on generic host volatiles. detection and perception of such compounds would provide the mosquito with a flexible, yet constrained, odour coding system that could delineate host preference. in this study, we show that the quintessential generic volatile for host- ...201728573028
a potent larvicidal agent against aedes aegypti mosquito from cardanol.cardanol is a constituent of cashew nut shell liquid that presents larvicidal activity against aedes aegypti. the isolation of cardanol is somewhat troublesome, however, in this work we describe an efficient and inexpensive method to obtain it as a pure material. the compound was used as starting material to make chemical transformation leading to saturated cardanol, epoxides and, halohydrins. these derivatives were tested for toxicity against aedes aegypti larvae. the results showed that iodohy ...201728562821
a new in vitro bioassay system for the discovery and quantitative evaluation of mosquito repellents.mosquitoes vector many pathogens that cause human diseases. repellents play a significant role in reducing the risk of these diseases by preventing mosquito bites. in this paper, we are reporting an ali and khan (a & k), large cage in vitro bioassay system that can effectively be used to measure repellency of compounds against mosquitoes. the system uses temperature as a landing and feeding stimulus, and collagen as a feeding substrate. the minimum effective dose (med) of deet (n, n-diethyl-meta ...201728541424
rna-seq comparison of larval and adult malpighian tubules of the yellow fever mosquito aedes aegypti reveals life stage-specific changes in renal function.introduction: the life history of aedes aegypti presents diverse challenges to its diuretic system. during the larval and pupal life stages mosquitoes are aquatic. with the emergence of the adult they become terrestrial. this shifts the organism within minutes from an aquatic environment to a terrestrial environment where dehydration has to be avoided. in addition, female mosquitoes take large blood meals, which present an entirely new set of challenges to salt and water homeostasis. methods: to ...201728536536
 introducción. el virus del zika (zikv) es un flavivirus con envoltura, transmitido a los seres humanos principalmente por el vector aedes aegypti. la infección por zikv se ha asociado con un gran neurotropismo y con efectos neuropáticos, como el síndrome de guillain-barré en el adulto y la microcefalia fetal y posnatal, así como con un síndrome de infección congénita similar al producido por el virus de la rubéola (rv).objetivo. comparar las estructuras moleculares de la proteína de envoltura e ...201728527274
quantitative structure-activity relationship study of amide mosquito repellents.a quantitative structure-activity relationship (qsar) study on 43 amide repellents was carried out by the heuristic method in order to reveal the correlations between molecular parameters of these amides and their repellency against aedes aegypti. sketches and optimizations of molecular structures were achieved by the gaussian software package. generation and screening of molecular parameters were accomplished using codessa 2.7.10 software. the leave-one-out method was applied for the model vali ...201728521600
heat in the southeastern united states: characteristics, trends, and potential health impact.high summer temperatures in extratropical areas have an impact on the public's health, mainly through heat stress, high air pollution concentrations, and the transmission of tropical diseases. the purpose of this study is to examine the current characteristics of heat events and future projections of summer apparent temperature (at)-and associated health concerns-throughout the southeastern united states. synoptic climatology was used to assess the atmospheric characteristics of extreme heat day ...201728520817
laboratory and semi-field evaluations of two (transfluthrin) spatial repellent devices against aedes aegypti (l.) (diptera: culicidae).two transfluthrin-based spatial repellent products (raid dual action insect repellent and home freshener and raid shield (currently not commercially available), sc johnson, racine wi) were evaluated for spatial repellent effects against female aedes aegypti (l.) mosquitoes under laboratory (wind tunnel) and semi-field (outdoor enclosure) conditions. the placement of either product in the wind tunnel significantly reduced host-seeking behaviors. the mean baseline (control) landing counts for the ...201728511270
reemergence of yellow fever in ethiopia after 50 years, 2013: epidemiological and entomological investigations.yellow fever (yf) is a viral hemorrhagic disease transmitted by aedes mosquito species. approximately, 200,000 cases and 30,000 deaths occur worldwide every year. in ethiopia, the last outbreak was reported in 1966 with 2200 cases and 450 deaths. a number of cases with deaths from unknown febrile illness reported from south ari district starting from november 2012. this investigation was conducted to identify the causative agent, source of the outbreak and recommend appropriate interventions.201728506254
aedes aegypti (l.) mosquitoes in trinidad, west indies: longevity case studies.few laboratory and field studies have reported long survival periods for ae. aegypti females and even fewer have designed experiments to characterize this important life history trait. this study was conducted under laboratory conditions to determine the number of blood meals taken by individual females, the number of eggs laid per individual female, the length of the gonotrophic cycle, and the duration of female survival. the results showed individual females oviposited between 670 and 1,500 eg ...201728504438
influence of shading and pedestrian traffic on the preference of aedes (stegomyia) aegypti (diptera: culicidae) for oviposition microenvironments.aedes aegypti mosquitoes are highly adaptable to abiotic stimuli. to evaluate the influence of shading and pedestrian traffic on the preference of ae. aegypti for oviposition microenvironments, 20 sites were sampled weekly using ovitraps within the perimeter of universidade federal do espírito santo, located in são mateus, espírito santo, brazil. a spatial and statistical analysis was performed in order to assess the relationship between shading time, pedestrian traffic, and the presence of biol ...201728504433
isolation of an insulin-like peptide from the asian malaria mosquito, anopheles stephensi, that acts as a steroidogenic gonadotropin across diverse mosquito taxa.many insulin-like peptides (ilps) have been identified in insects, yet only a few were isolated in their native form for structural and functional studies. antiserum produced to ilp3 in aedes aegypti was used in a radioimmunoassay to monitor the purification of an ilp from heads of adult an. stephensi and recognized the ilp in other immunoassays. the structure of the purified peptide matched that predicted for the ilp3 in this species. the native form stimulated ecdysteroid production by ovaries ...201728502740
metazoan ribosome inactivating protein encoding genes acquired by horizontal gene transfer.ribosome inactivating proteins (rips) are rna n-glycosidases that depurinate a specific adenine residue in the conserved sarcin/ricin loop of 28s rrna. these enzymes are widely distributed among plants and their presence has also been confirmed in several bacterial species. recently, we reported for the first time in silico evidence of rip encoding genes in metazoans, in two closely related species of insects: aedes aegypti and culex quinquefasciatus. here, we have experimentally confirmed the p ...201728500327
larval interference competition between the native neotropical mosquito limatus durhamii and the invasive aedes aegypti improves the fitness of both species.interspecific competition with native species during biological invasions can sometimes limit alien expansion. we aimed to determine the potential ecological effects of limatus durhamii theobald 1901, a native neotropical mosquito (diptera: culicidae) species, on the invasive species aedes (stegomyia) aegypti (linnaeus 1762) that breeds in the same artificial water containers. development time and adult dry mass were measured in 3 rearing conditions: control (a single larva), intraspecific compe ...201728497885
assessing the effect of selection with deltamethrin on biological parameters and detoxifying enzymes in aedes aegypti (l.).resistance to insecticides through one or several mechanisms has a cost for an insect in various parameters of its biological cycle. the present study evaluated the effect of deltamethrin on detoxifying enzymes and biological parameters in a population of aedes aegypti selected for 15 generations. the enzyme activities of alpha- and beta-esterases, mixed-function oxidases and glutathione-s-transferases were determined during selection, along with biological parameters.201728485040
what does heat tell a mosquito? characterization of the orientation behaviour of aedes aegypti towards heat sources.the use of heat as a cue for the orientation of haematophagous insects towards hot-blooded hosts has been acknowledged for many decades. in mosquitoes, thermoreception has been studied at the molecular, physiological and behavioural levels, and the response to heat has been evaluated in multimodal contexts. however, a direct characterization of how these insects evaluate thermal sources is still lacking. in this study we characterize aedes aegypti thermal orientation using a simple dual choice p ...201728479122
eco-friendly synthesis of silver nanoparticles and its larvicidal property against fourth instar larvae of aedes aegypti.in this study, larvicidal activity of silver nanoparticles (agnps) synthesised using apple extract against fourth instar larvae of aedes aegypti was determined. as a result, the agnps showed moderate larvicidal effects against ae. aegypti larvae (lc50 = 15.76 ppm and lc90 = 27.7 ppm). in addition, comparison of larvicidal activity performance of agnps at high concentration prepared using two different methods showed that ae. aegypti larvae was fully eliminated within the duration of 2.5 h. from ...201728476997
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