Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
[effects of methylprednisolone pulses in rheumatoid arthritis]. 20133724262
[female hormonal contraception]. 20133639613
primary care physicians' errors in handling cutaneous disorders. a prospective survey.this study analyzes the errors made by primary care physicians in handling skin disorders in patients seen prospectively over a 20-month period in a dermatologic practice. there were 319 errors in 260 patients. eighty-eight percent of the errors were in diagnosis. there was a striking tendency to overdiagnose infectious dermatoses such as bacterial pyodermas, superficial mycoses, scabies, and herpes simplex and to underdiagnose inflammatory dermatoses such as contact dermatitis, nummular dermati ...20133624563
assay for epinephrine and its impurities using reversed-phase high-performance liquid chromatography. application to control of an eye-drop solution. 20133624383
hours of the future: 12 hour shifts. 20133679276
[method of reporting dose distributions in teleradiotherapy for use in computer-based planning]. 20133613928
developing decision support systems for nursing. theoretical bases for advanced computer systems. 20133649263
[roentgen diagnosis of colorectal endometriosis].preoperative diagnosis of symptomatic colorectal endometriosis is often difficult. hence, the x-ray findings of eight woman patients with confirmed affection of the colon with endometriosis foci were evaluated together with the clinical, surgical and histological findings. in 50% of the cases rectal haemorrhages were the most frequently occurring sign. in three-quarters of the cases the colon sigmoideum was involved. radiologically it was possible to prove in 37.5% each of the cases that there w ...20133602868
fecal chymotrypsin levels in children with pancreatic insufficiency.the fecal chymotrypsin (fc) levels in samples collected over 24 h were determined by a new commercial colorimetric method from boehringer mannheim in 82 children suffering from various pancreatic disorders. the patients were divided into 4 groups, in accordance with the following etiologies: cystic fibrosis of the pancreas (cfp), chronic severe hepatic disorders (csh), primary malabsorption syndrome (pms) and malnutrition due to nondigestive causes (m). the control group comprised 48 children of ...20133581467
[multiple peripheral emboli resulting from thrombosis of a st. jude aortic valve prosthesis].although thrombosis of st. jude valve prosthesis is rare, we report a case of multiple thromboembolism in a patient who had stopped anticoagulant therapy. the consequences were very severe since two abdominal operations (colonic resection) were necessary. since the patient was not compliant with anticoagulant therapy, aortic valve replacement was performed and a biologic valve implanted. the postoperative phase was long but the patient left the hospital after two months' treatment. this case aga ...20133589635
the role of multienzyme complexes in integration of cellular metabolism.the notion of the "primary block" of cellular metabolism designated as "metabolic system" is introduced. metabolic system is defined as a metabolic pathway which corresponds to the structurally ordered multienzyme complex. the complex of glycolytic enzymes which catalyzes the anaerobic reduction of glucose-6-phosphate with production of atp may serve as an example of metabolic system (this complex does not contain hexokinase). the complex is formed on thin filaments of i-band of the muscle fibre ...20133747556
[therapy of systemic vasculitis]. 20133622296
[a case of pulmonary arteriovenous fistula resembling a peripheral pulmonary artery aneurysm on pulmonary angiography]. 20133586438
nodular regenerative hyperplasia and focal nodular hyperplasia of the liver associated with severe cholestasis. report of a case with autopsy findings.nodular regenerative hyperplasia of the liver was found at liver biopsy and at autopsy in a 61-year-old woman with severe cholestasis. increased bilirubin levels persisted over a follow-up period of 4 years. microscopic findings included hyperplastic hepatocytic nodules, interlobular bile duct lesions, fibrous medial and intimal thickening of small arterial branches and portal veins, and recanalization of a thrombus in a major portal branch related to an area of focal nodular hyperplasia of the ...20133713453
trauma in tourist towns. 20133650336
microanalysis of respiratory air samples, a simple device for undergraduate demonstration. 20133666883
hematogenous spinal leptomeningeal metastasis: a unique ct enhancement pattern.a distinct ct enhancement pattern of leptomeningeal metastasis from a systemic malignancy is described, corresponding to the pathologic and myelographic patterns of this entity. the uniform total subarachnoid enhancement, simulating intrathecal contrast, heralded sheetlike tumor proliferation along the surface of the spinal cord in an asymptomatic patient. since the majority of hypervascular intraspinal abnormalities show focal enhancement with intravenous contrast, recognition of this pattern m ...20133562111
non-malignant chest x ray changes in patients with mesothelioma in a large cohort of asbestos insulation workers.to assess the prevalence of non-malignant chest x ray abnormalities in cases of mesothelioma 184 cases of mesothelioma (72 pleural and 112 peritoneal) which had occurred in a cohort of asbestos insulation workers followed up since 1967 were studied. chest x ray films of satisfactory quality, on which the presence or absence of non-malignant radiological changes indicating interstitial pulmonary fibrosis or pleural fibrosis or both, could be assessed with a high degree of certainty were available ...20133606969
[pathophysiology and treatment of thrombosis. (3) protein c deficiency and thrombosis]. 20133553376
[changes of calcium fractions in plasma, dialysate and ultrafiltrate during hemodialysis]. 20134665579
[comparison between depot terbutaline tablets and ordinary terbutaline tablets]. 20133547972
methyl isocyanate liquid and vapor permeation through selected respirator diaphragms and chemical protective clothing.initially, a study was undertaken to evaluate selected chemical protective clothing suitable for use by emergency response personnel confronted with methyl isocyanate (mic). twenty-two chemical protective clothing materials were tested against liquid methyl isocyanate. chemical permeation breakthrough times for these clothing materials demonstrate that only one of these garments can be considered as a candidate material against liquid mic. in a subsequent study, three chemical protective clothin ...20133591647
a new syndrome of brachydactyly of the hands and feet with duplication of the first toes. 20134469972
[primary oxalosis in adulthood. scintigraphic and radiologic documentation of oxalosis nephropathy, oxalosis arthro-/enthesiopathy and oxalosis cardiomyopathy--review of the literature].clinical, radiographic and scintigraphic abnormalities in primary oxalosis of a 52 years old female are demonstrated. severe oxalosis-nephropathy and oxalosis-arthropathy/enthesiopathy are shown. for the first time an intense myocardial uptake of 99 m tc-hdp and 99 m tc-mdp is described in connection with oxalosis. it is suggested that in the appropriate clinical setting this pattern may be suggestive of oxalosis-cardiomyopathy. review of literature.20133575693
[indications for the surgical treatment of atherosclerosis of the cerebral arteries]. 20133594545
stapedotomy and better hearing results at 4000 hz. 20133814402
rapid chromosome analysis and prenatal diagnosis using fluid from cystic hygromas. 20133657885
pol i transcription: which comes first, the end or the beginning? 20133536127
asians and medication. 20133849596
acute bacterial endocarditis in early infancy. 20133679615
lower levels of vitamin c and carotenes in plasma of cigarette smokers.plasma levels of vitamins a, c, and e, selenium, and carotenes were determined in 125 male cigarette smokers and 125 age- and race-matched nonsmokers. the smokers had a mean daily consumption of 30.6 cigarettes and a cumulative consumption of 22.8 pack years. plasma levels of vitamin c and total carotenes were significantly (p less than 0.05) lower in smokers than those of nonsmokers, while levels of vitamin a, selenium, and vitamin e were not significantly different between these two groups. si ...20133734276
human naip and mouse naip1 recognize bacterial type iii secretion needle protein for inflammasome activation.inflammasome mediated by central nucleotide-binding and oligomerization domain (nod)-like receptor (nlr) protein is critical for defense against bacterial infection. here we show that type iii secretion system (t3ss) needle proteins from several bacterial pathogens, including salmonella typhimurium, enterohemorrhagic escherichia coli, shigella flexneri, and burkholderia spp., can induce robust inflammasome activation in both human monocyte-derived and mouse bone marrow macrophages. needle protei ...201323940371
single cell measurements of vacuolar rupture caused by intracellular pathogens.shigella flexneri are pathogenic bacteria that invade host cells entering into an endocytic vacuole. subsequently, the rupture of this membrane-enclosed compartment allows bacteria to move within the cytosol, proliferate and further invade neighboring cells. mycobacterium tuberculosis is phagocytosed by immune cells, and has recently been shown to rupture phagosomal membrane in macrophages. we developed a robust assay for tracking phagosomal membrane disruption after host cell entry of shigella ...201323792688
subversion of inflammasome activation and pyroptosis by pathogenic bacteria.activation of the inflammasome occurs in response to a notably high number of pathogenic microbes and is a broad innate immune response that effectively contributes to restriction of pathogen replication and generation of adaptive immunity. activation of these platforms leads to caspase-1- and/or caspase-11-dependent secretion of proteins, including cytokines, and induction of a specific form of cell death called pyroptosis, which directly or indirectly contribute for restriction of pathogen rep ...201324324933
characterization of intracellular growth regulator icgr by utilizing transcriptomics to identify mediators of pathogenesis in shigella flexneri.shigella species gram-negative bacteria which cause a diarrheal disease, known as shigellosis, by invading and destroying the colonic mucosa and inducing a robust inflammatory response. with no vaccine available, shigellosis annually kills over 600,000 children in developing countries. this study demonstrates the utility of combining high-throughput bioinformatic methods with in vitro and in vivo assays to provide new insights into pathogenesis. comparisons of in vivo and in vitro gene expressio ...201323753632
deletion of pic results in decreased virulence for a clinical isolate of shigella flexneri 2a from china.shigella is a major pathogen responsible for bacillary dysentery, a severe form of shigellosis. severity of the disease depends on the virulence of the infecting strain. shigella pathogenicity is a multi-gene phenomenon, involving the participation of genes on an unstable large virulence plasmid and chromosomal pathogenicity islands.201323391153
[neurinoma of the cecum]. 20133951146
[neurinoma of the cecum]. 20133951146
development of a dental health test for adolescents. 20133870263
thermographic assessment of healed burn wounds. 20133843671
russell-silver syndrome and hypopituitarism. patient report and literature review.russell-silver syndrome (rss) is a sporadic form of prenatal onset dwarfism with typical facial features, variable asymmetry, and linear growth 3 to 4 sds below the mean. endocrinologic studies are usually normal; however, six cases of rss with growth hormone deficiency have been reported, three of which had additional pituitary abnormalities. we describe another case, a 7-year-old girl with rss and deficiencies of growth hormone, corticotropin, and thyroid-stimulating hormone. replacement thera ...20133946325
occupational risks for meningiomas of the cns in sweden.using the cancer-environment registry of sweden, which links cancer incidence (1961 to 1979) with census information (1960) for all employed individuals in sweden, a systematic, population-based assessment was made of the occurrence of meningiomas of the cns according to industrial and occupational classifications. statistically significant standardized incidence ratios (sir) between 5 and 6 for meningioma were observed among glass, porcelain, or ceramic workers of both sexes. sirs of similar ma ...20133819887
extracranial arterial reconstruction for chronic cerebral ischaemia. an audit of experience in the durban teaching hospitals.experience with 147 operations for extracranial cerebral arterial reconstruction in 122 patients over a 36-month period is presented. the commonest condition in white, black and indian patients was atherosclerosis, although 8 of the 23 black patients had nonspecific aorto-arteritis (takayasu's disease). operations within the mediastinum included bypass from the ascending aorta to major arterial branches (14), brachiocephalic endarterectomy (1), and replacement grafts of carotid (1) and subclavia ...20133787377
[value of intrauterine contraception].the rate of iud complications has been reduced by the development of iuds made out of copper and by the improvement of their shape. the number of women using iuds has increased steadily over the last 10 years. the negative aspects are circulatory problems, discharges, undesired pregnancies, and infection of the genitalia. the improved copper iud can remain effective for up to 7 years. there has been no correlation shown between the use of the iud and cancer of the endometrium or cervix. for iud ...20134049935
[value of intrauterine contraception].the rate of iud complications has been reduced by the development of iuds made out of copper and by the improvement of their shape. the number of women using iuds has increased steadily over the last 10 years. the negative aspects are circulatory problems, discharges, undesired pregnancies, and infection of the genitalia. the improved copper iud can remain effective for up to 7 years. there has been no correlation shown between the use of the iud and cancer of the endometrium or cervix. for iud ...20134049935
[characteristic behavior patterns of chronic schizophrenics during play therapy with ball passing]. 20134875565
[characteristic behavior patterns of chronic schizophrenics during play therapy with ball passing]. 20134875565
epidermoid carcinoma of the conjunctiva. 20133772137
blood volume, erythrokinetics and spleen function in thrombocythaemia.essential thrombocythaemia was diagnosed in a series of 18 patients on the basis of platelet counts greater than 1,000 x 10(9)/1. radionuclide studies have been carried out to distinguish thrombocythaemia as a primary disease from polycythaemia vera, myelofibrosis and chronic granulocytic leukaemia presenting with high platelet counts. these have included blood volume and spleen function, and radio-iron (52fe) has been used to demonstrate the presence of extramedullary (splenic) erythropoiesis. ...20133933244
the relationship of clinical allergy and the chemical contamination of food. 20133759815
[topical aspects of professional medical morals]. 20133750030
address of the president. 20133866016
pain in the orbit: a feature of dural tap. 20133728920
postmenopausal estrogens in prevention of osteoporosis. benefit virtually without risk if cardiovascular effects are considered.postmenopausal estrogens can delay or prevent osteoporosis and decrease the frequency of fractures, but they also increase the risk of endometrial cancer. a decision analytic model was developed using a markov process with 18 different states to estimate quality-adjusted life expectancy with and without estrogen therapy. the model considered fractures of the hip, wrist, pelvis, humerus and spine with potential outcomes of short-term morbidity, long-term disability, nursing home placement, and de ...20133728509
[physiological parameters in simulated acute and chronic hypoxia]. 20133790303
halothane hepatitis in children. 20133728924
quantitation of cellular resolution in situ hybridization histochemistry in brain by image analysis.a new method for relative quantitation of mrna levels with single neuron resolution is described. hybridized tissue sections are emulsion dipped, exposed, and developed. the resulting silver grains are visualized using dark-field microscopy at high magnification. the method relies on computer-based image analysis of sequentially located, high resolution, small fields containing the cell images, each of which is analyzed for mrna content. maps of cell distributions are constructed, with cell mark ...20133696504
noninteraction of digoxin and nifedipine in cardiac patients. 20133700685
[extrahepatic bile duct carcinoma]. 20133660370
[distribution and clinical significance of lymphocyte subsets in patients with chronic aplastic anemia]. 20133652827
regional arterial chemotherapy of liver tumours. i. performance comparison between a totally implantable pump and a conventional access system.thirty-nine patients with liver tumours have been submitted to regional arterial chemotherapy by means of either totally implantable infusaid-400 pumps (22 c.) or implantable ports (17 c.). the latter were subsequently perfused with external pumps. there was one single major operative complication and no operative deaths. most patients underwent continuous fudr infusion. access related complications occurred in both groups. treatment was stopped for access related complications in 18.4% and 29.4 ...20133758367
[work time balance]. 20133640470
an approach to digital signal processing for ultrasonics research.the increasingly sophisticated techniques for the extraction of information from ultrasonic data entail a corresponding increase in complexity of the software used. an interactive signal and image processing package, called twodimsy, has been developed at the ultrasonics institute to allow non-programmers an entry to digital signal processing, and to simplify the task of staff involved in programming. we present an outline of the design considerations which shaped twodimsy, and a brief history o ...20133617236
[prenatal diagnosis of hemophilia a by dna analysis. first experience in spain]. 20133613722
myocardial localization of technetium-99m mdp in an infant. 20133698441
[effect of smoking on pregnancy]. 20133623251
ibuprofen does not affect fibrinolytic activity. 20133664167
the erectile angle: objective criterion to evaluate the papaverine test in impotence.intracavernous papaverine injection may be the first diagnostic step in the assessment of the impotent patient. however, the appreciation of its effect must rely on the evaluation of penile rigidity. since measurement of rigidity requires a sophisticated procedure that may not be available to the majority of practitioners, we attempted to find a simple and objective method to evaluate the results of this common test. we tested 50 patients complaining of impotence with intracavernous injection of ...20133669162
rounded atelectasis: considerations on its radiological diagnosis.rounded atelectasis is a particular form of peripheral lung collapse, adjacent to the pleura and causing a pulmonary opacity, often resembling a neoplastic mass. its early identification avoids further investigations with more invasive techniques, especially unnecessary thoracotomy. the aim of this report is to contribute some case studies to the body of information which characterizes this radiographic and tomodensitometric entity; aspiration biopsy can possibly be performed to resolve diagnost ...20133643828
vitamin d-induced hypercalcemia. 20133605151
[percutaneous urinary drainage. technic, results and indications].the various nephrostomy sets are described and the characteristics of a nephrostomy set adapted for urological practice are defined. the basic technique of percutaneous nephrostomy is described. the possible complications are reviewed together with the methods for their prevention and treatment. the current indications of percutaneous nephrostomy are also defined.20133566212
[bilateral orbital hematoma after external cardiac massage]. 20133576733
strategy of thyroid-function testing, a comparative study using tt4, ft4i, various ft4 and irma-tsh kits. 20133559057
hours of direct nursing care. assessing baseline data for an automated system. 20133646908
eata, an immunogenic protective antigen of enterotoxigenic escherichia coli, degrades intestinal mucin.enterotoxigenic escherichia coli (etec) is a major cause of morbidity and mortality due to infectious diarrhea in developing countries for which there is presently no effective vaccine. a central challenge in etec vaccinology has been the identification of conserved surface antigens to formulate a broadly protective vaccine. here, we demonstrate that eata, an immunogenic secreted serine protease of etec, contributes to virulence by degrading muc2, the major protein present in the small intestina ...201324478066
shigella flexneri 3a outer membrane protein c epitope is recognized by human umbilical cord sera and associated with protective activity.shigella flexneri 3a is one of the five major strains of the shigella genus responsible for dysentery, especially among children, in regions of high poverty and poor sanitation. the outer membrane proteins (omp) of this bacterium elicit immunological responses and are considered a prime target for vaccine development. when injected into mice they elicit a protective immunological response against a lethal dose of the pathogen. the omps from s. flexneri 3a were isolated and resolved by two-dimens ...201323940590
an alternative outer membrane secretion mechanism for an autotransporter protein lacking a c-terminal stable core.autotransporter (at) proteins are a broad class of virulence factors from gram-negative pathogens. at outer membrane (om) secretion appears simple in many regards, yet the mechanism that enables transport of the central at 'passenger' across the om remains unclear. om secretion efficiency for two at passengers is enhanced by approximately 20 kda stable core at the c-terminus of the passenger, but studies on a broader range of at proteins are needed in order to determine whether a stability diffe ...201324118465
bacteremia in patients with acute pancreatitis as revealed by 16s ribosomal rna gene-based techniques*.to define the characteristic of bacteremia in patients with acute pancreatitis and determine its possible association with the disease severity.201323863226
bacteremia in the patients with acute pancreatitis as revealed by 16s ribosomal rna gene-based techniques.objectives:: to define the characteristic of bacteremia in the patients with acute pancreatitis and determine its possible association with the disease severity. design:: a prospective controlled study. setting:: icu of jinling hospital, china. patients:: a total of 48 patients with mild or severe acute pancreatitis were enrolled in this study. interventions:: none. measurements and main results:: samples of peripheral blood were collected from the patients at 4 or 5 and 9 or 10 days after acute ...201323764972
synthesis and concentration dependent antibacterial activities of cuo nanoflakes.we report, synthesis and antibacterial activities of cuo nanoflakes. cuo nanoparticles are prepared at room temperature through sol-gel method. x-ray diffraction studies show the particles are monoclinic (crystalline) in nature. scanning electron microscopy (sem) images clearly show that the prepared particles are flake like in structure. fourier transform infrared (ftir) spectra exhibits three different bands that correspond to the au and bu modes. antibacterial studies were performed on shigel ...201323498227
synthesis, spectroscopic, molecular orbital calculation, cytotoxic, molecular docking of dna binding and dna cleavage studies of transition metal complexes with n-benzylidene-n'-salicylidene-1,1-diaminopropane.eight mononuclear chromium(iii), manganese(ii), iron(iii), cobalt(ii), nickel(ii), copper(ii), zinc(ii) and cadmium(ii) complexes of schiff's base ligand were synthesized and determined by different physical techniques. the complexes are insoluble in common organic solvents but soluble in dmf and dmso. the measured molar conductance values in dmso indicate that the complexes are non-electrolytic in nature. all the eight metal complexes have been fully characterized with the help of elemental ana ...201323831943
multiplex pcr (polymerase chain reaction) assay for detection of e. coli o157:h7, salmonella sp., vibrio cholerae and vibrio parahaemolyticus in spiked shrimps (penaeus monodon).the coastal aquaculture mainly shrimps constitute major export sector in bangladesh and is increasingly shaped by international trade conditions and by national responses to those stringent quality and safety standards. pcr based validated methods for detection of major bacterial pathogens in shrimp might be very useful tool for ensuring quality and safety standards of exportable shrimps. the objective of this study was to evaluate overall performance (sensitivity and specificity) of the multipl ...201324498789
combination of extracts from aristolochia cymbifera with streptomycin as a potential antibacterial drug.the appearance of new antibiotic-resistant bacteria is a societal problem that requires the development of new alternative treatments. therefore, this work evaluated the antibacterial activity of ethanolic (ehi), dichloromethanic (edi) and hexanic (ehe) extracts from aristolochia cymbifera stems and the combination of these extracts with an antimicrobial drug to develop a new antibacterial therapy. the edi, ehe and ehi extracts were obtained by maceration using three different solvents. the mini ...201324040585
screening of solvent dependent antibacterial activity of prunus domestica.fruit of prunus domestica was extracted in ethanol. the ethanol extract was further extracted with two solvents ethyl acetate and chloroform. the crude ethanol extract and two fractions (ethyl acetate and chloroform) were screened for their antibacterial activity using the agar well diffusion method .they were tested against nine bacteria; five gram positive bacteria (staphylococcus aureus, streptococcuc intermedius, bacillus cereus, bacillus pumilus) and four gram negative bacteria (eschrichia ...201323455215
expression of shigella flexneri ipab gene in tobacco.shigellosis is a leading cause of diarrhea in many developing countries and although the disease can be controlled and managed with antibiotics, the constant emergence of resistant species requiring ever newer antibacterial drugs make development of an effective vaccine necessary. the bacteria are highly contagious and since immunity to shigella is serotype-specific a multi-serotype vaccine is required for adequate protection. proteins encoded by shigella invasion plasmid, which are part of the ...201323799180
antimicrobial and antioxidant activities of alcoholic extracts of rumex dentatus l.in-vitro antimicrobial and antioxidant activities of various concentrations ranging from 150 to 500 μg/ml of alcoholic (methanol and ethanol) extracts of rumex dentatus were analyzed on different clinical bacterial strains (shigella flexneri, klebsiella pneumoniae, escherichia coli, pseudomonas aeruginosa, salmonella typhimurium, staphylococcus aureus) and fungal strains (aspergillus versicolor, aspergillus flavus, acremonium spp., penicillium dimorphosporum, candida albicans, candida kruesie, c ...201323415966
isolation and characterization of antimicrobial constituents of searsia chirindensis l. (anacardiaceae) leaf extracts.searsia chirindensis is used in south african traditional medicine for management of bacterial infections such as diarrhoea. aim of the study was to examine the phytochemical composition from the leaves of searsia chirindensis that is responsible for the ethnomedicinal use of this plant.201324060408
immobilization of lysozyme-clea onto electrospun chitosan nanofiber for effective antibacterial applications.chitosan (cs) nanofibers with a diameter of 150-200nm were fabricated from a mixed chitosan/poly (vinyl alcohol) (pva) solution by the electrospinning method. the nascent cs/pva nanofibers were treated with 0.5m naoh solution to make stable cs nanofibers by removing pva under aqueous conditions. hen egg-white lysozyme was immobilized on electrospun cs nanofibers via cross-linked enzyme aggregates (cleas) and used for effective and continuous antibacterial applications. the maximum amount of lyso ...201323201775
molecular structure, normal coordinate analysis, harmonic vibrational frequencies, natural bond orbital, td-dft calculations and biological activity analysis of antioxidant drug 7-hydroxycoumarin.in this work, we report harmonic vibrational frequencies, molecular structure, nbo and homo, lumo analysis of umbelliferone also known as 7-hydroxycoumarin (7hc). the optimized geometric bond lengths and bond angles obtained by computation (monomer and dimmer) shows good agreement with experimental xrd data. harmonic frequencies of 7hc were determined and analyzed by dft utilizing 6-311+g(d,p) as basis set. the assignments of the vibrational spectra have been carried out with the help of normal ...201323123244
synthesis, biological evaluation and molecular docking studies of some pyrimidine derivatives.some novel pyrimidine-5-carbonitrile derivatives bearing various substituent have been synthesized. the structures of target compounds were confirmed by elemental analysis and spectral data. some selected members of the newly synthesized compounds were investigated for their cytotoxic potency against certain human tumor cell lines. five representative active anticancer compounds 6a, 6c, 6d, 17a and 18a were subjected to docking using moe program on the 3d structure of two enzymes, namely; thymid ...201323811090
a creb3-arf4 signalling pathway mediates the response to golgi stress and susceptibility to pathogens.treatment of cells with brefeldin a (bfa) blocks secretory vesicle transport and causes a collapse of the golgi apparatus. to gain more insight into the cellular mechanisms mediating bfa toxicity, we conducted a genome-wide haploid genetic screen that led to the identification of the small g protein adp-ribosylation factor 4 (arf4). arf4 depletion preserves viability, golgi integrity and cargo trafficking in the presence of bfa, and these effects depend on the guanine nucleotide exchange factor ...201324185178
noncanonical inflammasome activation by intracellular lps independent of tlr4.gram-negative bacteria including escherichia coli, citrobacter rodentium, salmonella typhimurium, and shigella flexneri are sensed in an ill-defined manner by an intracellular inflammasome complex that activates caspase-11. we show that macrophages loaded with synthetic lipid a, e. coli lipopolysaccharide (lps), or s. typhimurium lps activate caspase-11 independently of the lps receptor toll-like receptor 4 (tlr4). consistent with lipid a triggering the noncanonical inflammasome, lps containing ...201323887873
the host and the flora.to prevent bacterial overgrowth, colonization of the epithelium and subsequent translocation, the gastrointestinal tract maintains an effective mucosal barrier. besides mucus the most important components of this protective system are epithelial antimicrobial peptides such as defensins, the cathelicidin ll-37, lysozyme, phospholipase a, and proteins with additional antimicrobial properties such as ubiquicidin, ribosomal proteins or histones. commensal species may tolerate intestinal antimicrobia ...201324246976
transposition of isecp1 modulates blactx-m-55-mediated shigella flexneri resistance to cefalothin.the aim of this study was to uncover the mechanisms underlying shigella flexneri resistance to cefalothin. in this study, a resistance-related s. flexneri isolate, s. flexneri ydc, was obtained from s. flexneri mel-1998023/zz pre-incubated with cefalothin at a dose of 0.5 × the minimum inhibitory concentration. the isecp1 coding element was identified upstream of bla(ctx-m-55) in s. flexneri ydc. to further determine the role of isecp1 in s. flexneri resistance, plasmids containing bla(ctx-m-55) ...201324207017
role of porin proteins in acquisition of transferrin iron by enteropathogens.acquisition of iron from key innate immune defence proteins such as transferrin (tf) and lactoferrin is an important mechanism by which pathogenic bacteria obtain essential iron for growth within their host. bacterial species that do not produce siderophores often use specific tf-binding proteins, the best characterized being the neisseriaceae-type tf-binding proteins tbpa and tbpb. previous work from our laboratory has shown that siderophore-producing enteric species such as escherichia coli al ...201324089578
small-molecule inhibitor of the shigella flexneri master virulence regulator virf.virf is an arac family transcriptional activator that is required for the expression of virulence genes associated with invasion and cell-to-cell spread by shigella flexneri, including multiple components of the type three secretion system (t3ss) machinery and effectors. we tested a small-molecule compound, se-1 (formerly designated ossl_051168), which we had identified as an effective inhibitor of the arac family proteins rhas and rhar, for its ability to inhibit virf. cell-based reporter gene ...201324002059
o-antigen protects gram-negative bacteria from histone killing.beyond their traditional role of wrapping dna, histones display antibacterial activity to gram-negative and -positive bacteria. to identify bacterial components that allow survival to a histone challenge, we selected resistant bacteria from homologous escherichia coli libraries that harbor plasmids carrying pieces of the chromosome in different sizes. we identified genes required for exopolysaccharide production and for the synthesis of the polysaccharide domain of the lipopolysaccharide, called ...201323951089
modulators of antibiotic activity from ipomoea murucoides.reinvestigation of the chcl3-soluble extract from the flowers of ipomoea murucoides, through preparative-scale recycling hplc, yielded three pentasaccharides of 11-hydroxyhexadecanoic acid, murucoidins xvii-xix, in addition to the known murucoidin iii and v, all of which were characterized by nmr spectroscopy and mass spectrometry. these compounds were found to be macrolactones of the known pentasaccharides simonic acid b and operculinic acid a. the acylating groups corresponded to acetic, (2s)- ...201323920226
oxalis corniculata (oxalidaceae) leaf extract exerts in vitro antimicrobial and in vivo anticolonizing activities against shigella dysenteriae 1 (nt4907) and shigella flexneri 2a (2457t) in induced diarrhea in suckling mice.in this study, the extract of a green leafy vegetable oxalis corniculata (oxalidaceae) was evaluated for its in vitro antibacterial and in vivo anti colonizing effect against common intestinal pathogenic bacteria. methanolic extract (80%) of oxalis corniculata (oxalidaceae) leaf contained a polyphenol content of 910 mg gallic acid equivalent per gram of dry weight and the yield was 8%. the flavonoid content was 2.353 g quercetin equivalent per 100 g of the extract. in vitro studies indicated tha ...201323909903
effects of subtherapeutic concentrations of antimicrobials on gene acquisition events in yersinia, proteus, shigella, and salmonella recipient organisms in isolated ligated intestinal loops of swine.to assess antimicrobial resistance and transfer of virulence genes facilitated by subtherapeutic concentrations of antimicrobials in swine intestines.201323879845
Refresh
firstprev
...11121314151617181920...
nextlast
Displaying items 1501 - 1600 of 11070