Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| subgingival occurrence of enteric rods, yeasts and staphylococci after systemic doxycycline therapy. | the occurrence of subgingival enteric rods, yeasts and staphylococci (pre-treatment and post-treatment) was examined in 21 adult periodontitis patients receiving mechanical periodontal therapy and systemic doxycycline (200 mg on first day, then 100 mg/day for 20 days). subgingival samples obtained prior to, and 2 weeks and 4 weeks after completion of the antibiotic therapy were transported in vmga iii and plated onto tsbv (for enteric rods and yeasts) and staphylococcus 110 media. at baseline, s ... | 1990 | 2080072 |
| isolation of enterobacter aerogenes susceptible to beta-lactam antibiotics despite high level beta-lactamase production. | this report describes a patient with nosocomial meningitis from whom four distinct isolates of enterobacter aerogenes were recovered over a complicated course of chemotherapy. the initial isolate was susceptible to expanded spectrum beta-lactams despite constitutive production of high levels of beta-lactamase. resistant isolates recovered during antibiotic therapy had lost a 42,000 outer membrane protein. these data suggest that b-lactam susceptibility in the original isolate was due to "hyperpe ... | 1990 | 2086219 |
| [susceptibilities of clinical bacterial isolates to antimicrobial agents. a study mainly focused on imipenem. research group for testing imipenem susceptibility on clinical isolates]. | we investigated susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antimicrobial agents at 459 hospital laboratories throughout japan from september to december of 1988. in this study, identification and susceptibility testing were performed at each hospital laboratory and the tests were carried out according to the 1-dilution or 3-dilution disc technique in which susceptibilities are classified into 4 grades: , ++, + and -. ipm had significantly high activity against st ... | 1990 | 2086814 |
| new screening test to determine the acceptability of 0.45-micron membrane filters for analysis of water. | during routine membrane filter (mf) quality control testing, irregularities such as partial or complete inhibition of microbial growth at grid lines, abnormal spreading of colonies, growth in or along the grid lines, nonwetting areas, poor colony sheen and metallic sheen on the mf surface with mendo agar, brittleness, decreased recovery, and severe wrinkling were observed with several lots of filters. to study these effects and to develop a more sensitive screening test for mf quality, we compar ... | 1990 | 2106827 |
| mucinophilic and chemotactic properties of pseudomonas aeruginosa in relation to pulmonary colonization in cystic fibrosis. | representative isolates of nonmucoid pseudomonas aeruginosa were studied to investigate the hypothesis that mucinophilic and chemotactic properties in this species act as potential factors in the initial stages of pulmonary colonization in patients with cystic fibrosis (cf). transmission electron microscopy with a surfactant monolayer technique was used in a novel manner to demonstrate the adhesion of all 10 p. aeruginosa strains examined to porcine gastric mucin and tracheobronchial mucin from ... | 1990 | 2111280 |
| nucleotide sequence of the pseudomonas fluorescens signal peptidase ii gene (lsp) and flanking genes. | the lsp gene encoding prolipoprotein signal peptidase (signal peptidase ii) is organized into an operon consisting of iles and three open reading frames, designated genes x, orf149, and orf316 in both escherichia coli and enterobacter aerogenes. a plasmid, pbroc128, containing a 5.8-kb fragment of pseudomonas fluorescens dna was found to confer pseudomonic acid resistance on e. coli host cells and to contain the structural gene of iles from p. fluorescens. in addition, e. coli strains carrying p ... | 1990 | 2121716 |
| [ecological changes in burn wound bacterial flora]. | a comparative study of the ecology of the burn wound bacterial flora, during jan. 1970 to dec. 1971 (group i) and jan. 1985 to dec. 1986 (group ii) was carried out. the incidence of gram positive cocci was 40.70% in group i versus 45.66% in group ii. that of gram negative bacilli was 54.92% and 48.10% respectively. the occurrence of ps. aeruginosa was markedly reduced, in group i it reached 35.08% versus 11.26% in group ii (p less than 0.001). the incidence of enterobacter aerogenes was signific ... | 1990 | 2125851 |
| [susceptibility and r plasmids conjugation tests for 44 strains of gram negative bacilli in a burn unit]. | 44 strains of 9 species gram negative bacilli were isolated and identified in a burn unit, among them 25 strains were from patients and 19 from ward environment. all strains were tested for susceptibility to antibiotics and r plasmids. using both agar dilution and disc diffusion methods to test susceptibility to 12 kinds of antibiotics, namely, streptomycin chloramphenicol, tetracycline, ampicillin, kanamycin, gentamycin, nalidixic acid, amikacin rifampin, carbenicillin, cefazolin and polymyxin, ... | 1990 | 2125853 |
| growth inhibition of selected food-borne bacteria, particularly listeria monocytogenes, by plant extracts. | six extracts from chinese medicinal plants: tin men chu, sey lau pai, siu mao heung, bak tao yung, kam chin chiu and liao ya, were tested for their inhibitory effect on selected food-borne bacteria by the well assay technique. among them, tin men chu, siu mao heung and sey lau pai inhibited the growth of staphylococcus aureus, klebsiella pneumonia, escherichia coli, shigella flexneri, streptococcus faecalis, salmonella paratyphi, salm. enteritidis, enterobacter aerogenes, pseudomonas fluorescens ... | 1990 | 2127264 |
| purification, characterization, and in vivo reconstitution of klebsiella aerogenes urease apoenzyme. | urease was purified from recombinant klebsiella aerogenes which was grown in the absence of nickel. the protein was inactive and contained no transition metals, yet it possessed the same heteropolymeric structure as native enzyme, demonstrating that ni is not required for intersubunit association. ni did, however, substantially increase the stability of the intact metalloprotein (tm = 79 degrees c) compared with apoenzyme (tm = 62 degrees c), as revealed by differential scanning calorimetric ana ... | 1990 | 2142939 |
| ofloxacin versus vancomycin/polymyxin for prevention of infections in granulocytopenic patients. | the efficacy and safety of oral ofloxacin were compared with those of vancomycin/polymyxin for prophylaxis of bacterial infections in granulocytopenic patients undergoing chemotherapy for hematologic malignancy. | 1990 | 2153006 |
| cloning of pseudomonas aeruginosa algg, which controls alginate structure. | the biochemical mechanism by which alpha-l-guluronate (g) residues are incorporated into alginate by pseudomonas aeruginosa is not understood. p. aeruginosa first synthesizes gdp-mannuronate, which is used to incorporate beta-d-mannuronate residues into the polymer. it is likely that the conversion of some beta-d-mannuronate residues to g occurs by the action of a c-5 epimerase at either the monomer (e.g., sugar-nucleotide) or the polymer level. this study describes the results of a molecular ge ... | 1990 | 2160929 |
| tn1000-mediated insertion mutagenesis of the histidine utilization (hut) gene cluster from klebsiella aerogenes: genetic analysis of hut and unusual target specificity of tn1000. | the histidine utilization (hut) genes from klebsiella aerogenes were cloned in both orientations into the hindiii site of plasmid pbr325, and the two resulting plasmids, pcb120 and pcb121, were subjected to mutagenesis with tn1000. the insertion sites of tn1000 into pcb121 were evenly distributed throughout the plasmid, but the insertion sites into pcb120 were not. there was a large excess of tn1000 insertions in the "plus" or gamma delta orientation in a small, ca. 3.5-kilobase region of the pl ... | 1990 | 2170334 |
| a sulfur- and tyramine-regulated klebsiella aerogenes operon containing the arylsulfatase (atsa) gene and the atsb gene. | the structural gene for arylsulfatase (atsa) of klebsiella aerogenes was cloned into a pki212 vector in escherichia coli. deletion analysis showed that the atsa gene with the promoter region was located within a 3.2-kilobase cloned segment. in e. coli cells which carried the plasmid, the synthesis of arylsulfatase was repressed by various sources of sulfur; the repression was relieved, in each case, by tyramine. transfer of the plasmid into atsa or constitutive atsr mutant strains of k. aerogene ... | 1990 | 2180918 |
| molecular characterization of pula and its product, pullulanase, a secreted enzyme of klebsiella pneumoniae unf5023. | the determined nucleotide sequence of the klebsiella pneumoniae unf5023 gene pula comprises a single open reading frame coding for a 1090-residue precursor of the secreted protein pullulanase. the predicted sequence of this protein is highly homologous to that of pullulanase of klebsiella aerogenes strain w70. however, the unf5023 pullulanase lacks a collagen-like sequence present at the n-terminus of the mature w70 enzyme and differs further from the w70 pullulanase around residue 300 and at th ... | 1990 | 2181242 |
| oral ciprofloxacin compared with parenteral antibiotics in the treatment of osteomyelitis. | we undertook a prospective, randomized comparison of oral ciprofloxacin with standard parenteral therapies for the treatment of biopsy-proven osteomyelitis caused by susceptible organisms. following surgical debridement, the ciprofloxacin patients received 750 mg twice a day, and the other patients received a broad-spectrum cephalosporin or a nafcillin-aminoglycoside combination intravenously (i.v.). there were 31 evaluable patients in the ciprofloxacin group, treated an average of 56 days, and ... | 1990 | 2183710 |
| electroporation and expression of plasmid pbr322 in klebsiella aerogenes nctc 418 and plasmid prk2501 in pseudomonas putida cym 318. | klebsiella aerogenes nctc 418 and pseudomonas putida cym 318 were transformed via high-voltage electroporation with plasmids pbr322 and prk2501, respectively. the number of transformants obtained was dependent on the applied voltage, capacitance, and cell recovery procedure. for example, 7.87 x 10(4) transformants/micrograms dna were obtained at 2500 v, 25 muf when k. aerogenes cells were electroporated with pbr322 dna. a lower voltage (1500) and capacitance (3 muf) yielded 2.4 x 10(3) transform ... | 1990 | 2187074 |
| purification and characterization of urease from helicobacter pylori. | urease was purified 112-fold to homogeneity from the microaerophilic human gastric bacterium, helicobacter pylori. the urease isolation procedure included a water extraction step, size exclusion chromatography, and anion exchange chromatography. the purified enzyme exhibited a km of 0.3 +/- 0.1 mm and a vmax of 1,100 +/- 200 mumols of urea hydrolyzed/min/mg of protein at 22 degrees c in 31 mm tris-hcl, ph 8.0. the isoelectric point was 5.99 +/- 0.03. molecular mass estimated for the native enzym ... | 1990 | 2188975 |
| kinetics and specificity of alginate lyases: part i, a case study. | purified preparations of alginate lyase from klebsiella aerogenes and haliotis sp. were investigated for activity and degradation patterns with alginate and alginate fragments having different compositions and sequences. with fragments approaching homopolymers of guluronate and mannuronate, michaelis-menten kinetics were obeyed and kinetic parameters could be obtained. degradation of alginates containing all four possible linkages in various proportions, followed by isolation of the fragments an ... | 1990 | 2191774 |
| cloning of aldb, which encodes alpha-acetolactate decarboxylase, an exoenzyme from bacillus brevis. | a gene for alpha-acetolactate decarboxylase (aldc) was cloned from bacillus brevis in escherichia coli and in bacillus subtilis. the 1.3-kilobase-pair nucleotide sequence of the gene, aldb, encoding aldc and its flanking regions was determined. an open reading frame of 285 amino acids included a typical n-terminal signal peptide of 24 or 27 amino acids. a b. subtilis strain harboring the aldb gene on a recombinant plasmid processed and secreted aldc. in contrast, a similar enzyme from enterobact ... | 1990 | 2198252 |
| nucleotide sequence of the gene encoding the repressor for the histidine utilization genes of pseudomonas putida. | the hutc gene of pseudomonas putida encodes a repressor which, in combination with the inducer urocanate, regulates expression of the five structural genes necessary for conversion of histidine to glutamate, ammonia, and formate. the nucleotide sequence of the hutc region was determined and found to contain two open reading frames which overlapped by one nucleotide. the first open reading frame (orf1) appeared to encode a 27,648-dalton protein of 248 amino acids whose sequence strongly resembled ... | 1990 | 2203753 |
| nucleotide sequence of the gene encoding the repressor for the histidine utilization genes of klebsiella aerogenes. | the hutc gene of klebsiella aerogenes encodes a repressor that regulates expression of the histidine utilization (hut) operons. the dna sequence of a region known to contain hutc was determined and shown to contain two long rightward-reading open reading frames (orfs). one of these orfs was identified as the 3' portion of the hutg gene. the other orf was the hutc gene. the repressor predicted from the hutc sequence contained a helix-turn-helix motif strongly similar to that seen in other dna-bin ... | 1990 | 2203754 |
| sequence of the klebsiella aerogenes urease genes and evidence for accessory proteins facilitating nickel incorporation. | a 4.8-kilobase-pair region of cloned dna encoding the genes of the klebsiella aerogenes urease operon has been sequenced. six closely spaced open reading frames were found: urea (encoding a peptide of 11.1 kilodaltons [kda]), ureb (11.7-kda peptide), urec (60.3-kda peptide), uree (17.6-kda peptide), uref (25.2-kda peptide), and ureg (21.9-kda peptide). immediately after the ureg gene is a putative rho-dependent transcription terminator. the three subunits of the nickel-containing enzyme are enco ... | 1990 | 2211515 |
| construction of mobilizable vectors derived from plasmids rp4, puc18 and puc19. | mobilizable narrow-host-range plasmids were constructed from puc18 and puc19 by addition of a segment of psup2021 bearing the basis of mobilization (bom) site and origin of transfer (orit) of rp4. one pair of expression vectors, paro180 and paro190, retains the beta-lactamase (bla) gene and twelve of the 13 restriction enzyme multiple cloning sites (mcs) of puc18/19. another pair was created by replacing the bla gene with the gene encoding kanamycin resistance (kan) from tn5. the molecules repli ... | 1990 | 2227423 |
| ammonia-induced cell envelope injury in escherichia coli and enterobacter aerogenes. | ammonia-induced cell envelope injury was examined in pure cultures of escherichia coli and enterobacter aerogenes. cell injury, as determined by the ratio of colony-forming units on m-t7 agar to colony-forming units on m-endo agar, increased with exposure to increasing concentrations of ammonia. cell envelopes appeared to be the site of injury as indicated by increasing susceptibility to lysozyme with increasing ammonia concentration. cells exposed to ammonia also exhibited more cellular leakage ... | 1990 | 2245377 |
| homology among bacterial catalase genes. | catalase activities in crude extracts of exponential and stationary phase cultures of various bacteria were visualized following gel electrophoresis for comparison with the enzymes from escherichia coli. citrobacter freundii, edwardsiella tarda, enterobacter aerogenes, klebsiella pneumoniae, and salmonella typhimurium exhibited patterns of catalase activity similar to e. coli, including bifunctional hpi-like bands and a monofunctional hpii-like band. proteus mirabilis, erwinia carotovora, and se ... | 1990 | 2253114 |
| cloning of the klebsiella aerogenes nac gene, which encodes a factor required for nitrogen regulation of the histidine utilization (hut) operons in salmonella typhimurium. | the nac (nitrogen assimilation control) gene from klebsiella aerogenes, cloned in a low-copy-number cloning vector, restored the ability of k. aerogenes nac mutants to activate histidase and repress glutamate dehydrogenase formation in response to nitrogen limitation and to limit the maximum expression of the nac promoter. when present in salmonella typhimurium, the k. aerogenes nac gene allowed the hut genes to be activated during nitrogen-limited growth. thus, the nac gene encodes a cytoplasmi ... | 1990 | 2254273 |
| regulation of assimilatory nitrate reductase formation in klebsiella aerogenes w70. | klebsiella aerogenes w70 could grow aerobically with nitrate or nitrite as the sole nitrogen source. the assimilatory nitrate reductase and nitrite reductase responsible for this ability required the presence of either nitrate or nitrite as an inducer, and both enzymes were repressed by ammonia. the repression by ammonia, which required the ntr (nitrogen regulatory) system (a. macaluso, e. a. best, and r. a. bender, j. bacteriol. 172:7249-7255, 1990), did not act solely at the level of inducer e ... | 1990 | 2254283 |
| treatment of community-acquired lower respiratory tract infections with oral cefuroxime axetil. | the subjects were adult hospitalized patients, 12 with pneumonia and eight with acute bronchitis. the patients with pneumonia received 500 mg of cefuroxime orally twice daily and the patients with bronchitis received 250 mg twice daily. treatment lasted for ten days in responsive patients. the pathogens identified in the patients' sputum were streptococcus pneumoniae, klebsiella pneumoniae, haemophilus influenzae, enterobacter aerogenes, staphylococcus aureus, or branhamella catarrhalis. clinica ... | 1990 | 2268866 |
| immunological cross-reactivity between enterobacter aerogenes and klebsiella capsular polysaccharides. | enterobacter aerogenes capsular polysaccharides (cps) which cross-react with klebsiella k3, k21, and k68 cps were purified. humans immunized with a vaccine containing klebsiella k3 and k21 cps produced antibody which recognized these e. aerogenes cps. the magnitude of the immune response to the e. aerogenes cps varied among the serotypes. passive transfer of an immunoglobulin produced from the plasma of donors immunized with the above vaccine afforded significant protection against challenge wit ... | 1990 | 2277587 |
| molecular cloning, expression and nucleotide sequence of the rcsa gene of erwinia amylovora, encoding a positive regulator of capsule expression: evidence for a family of related capsule activator proteins. | a gene encoding a positive activator of the expression of extracellular polysaccharide (eps) synthesis in the phytopathogen erwinia amylovora has been isolated from a genomic library in escherichia coli. the presence of the cloned gene in e. coli stimulated transcription of the genes encoding colanic acid biosynthesis and could complement rcsa mutations. introduction of the gene on a multicopy plasmid into er. amylovora caused a threefold increase in eps expression. the nucleotide sequence of th ... | 1990 | 2283503 |
| the sequence of the malg gene from salmonella typhimurium and its functional implications. | the nucleotide sequence of the malg gene which is essential for maltose transport was determined in salmonella typhimurium and compared to homologous genes from escherichia coli and enterobacter aerogenes. malg genes from s. typhimurium and e. aerogenes were expressed and their products were active in e. coli. the primary structure of the three malg proteins was highly conserved. changes were mainly clustered in a relatively large hydrophilic region of the protein (residues 40 to 75). in contras ... | 1990 | 2284499 |
| [susceptibility of clinically isolated bacterial strains to imipenem/cilastatin sodium]. | in vitro antibacterial activities of imipenem/cilastatin sodium (imipenem) and other beta-lactams against clinically isolated 353 bacterial strains were investigated. the results obtained in this study are summarized as follows: 1. imipenem (ipm) showed potent antibacterial activities against gram-positive cocci such as staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, streptococcus agalactiae. 2. ipm had inferior or equivalent antibacterial activities to beta-lactams aga ... | 1990 | 2287059 |
| [susceptibilities of clinical bacterial isolates to antimicrobial agents. a study mainly focused on imipenem. reported by the research group for testing imipenem susceptibility on clinical isolates]. | this study was conducted to investigate susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antibacterial agents at 64 hospital laboratories throughout japan from september to december of 1988. in this study, identification and susceptibility testing were carried out at each laboratory and the tests were performed according to the disk dilution method recommended by nccls in which susceptibilities are classified into "s", "ms", "i" and "r". ipm showed markedly high in vit ... | 1990 | 2287060 |
| in vitro activity against clinically important gram-positive and gram-negative bacteria of sulbactam, alone and in combination with ampicillin, cefotaxime, mezlocillin, and piperacillin. | the in vitro effects of the single agents, and the synergistic/antagonistic action of three different combinations of ampicillin (amp, cas 69-53-4), cefotaxime (ctx, cas 63527-52-6), mezlocillin (mez, cas 51481-65-3), and piperacillin (pip, cas 61477-96-1) with the beta-lactamase inhibitor sulbactam (sul, cas 68373-14-8) were determined against 675 gram-positive and gram-negative, both aerobic and anaerobic bacteria. all the combinations of sulbactam and the antibiotics (1: 1, 1:2 and 1:4) exhib ... | 1990 | 2291754 |
| purification and n-terminal analysis of urease from helicobacter pylori. | urease of helicobacter pylori (formerly campylobacter pylori) is believed to represent a critical virulence determinant for this species. ammonia generated by hydrolysis of urea may protect the acid-sensitive bacterium as it colonizes human gastric mucosa. an h. pylori strain, cultured from a gastric biopsy of a patient with complaints of abdominal pain and a history of peptic ulcer disease, was isolated on selective medium and cultured in mueller-hinton broth supplemented with 4% fetal calf ser ... | 1990 | 2318539 |
| enhanced resistance to cefotaxime and imipenem associated with outer membrane protein alterations in enterobacter aerogenes. | mutants exhibiting enhanced resistance to cefotaxime and imipenem were selected by plating a strain of enterobacter aerogenes, which already produced chromosomal beta-lactamase constitutively, on to varying concentrations of different beta-lactam antibiotics. frequencies of mutation varied from 10(-5) to 10(-8), depending upon the particular antibiotic and concentration used for selection. only minor variations in beta-lactamase specific activities were observed and these could not be directly c ... | 1990 | 2318754 |
| diverse potential of beta-lactamase inhibitors to induce class i enzymes. | the ability of various beta-lactamase inhibitors to induce class i beta-lactamases was assessed. clavulanate was the most active compound, inducing morganella morganii, aeromonas caviae, and enterobacter aerogenes over a broad concentration range and citrobacter freundii, pseudomonas aeruginosa, and serratia marcescens at high concentrations. disk approximation tests paralleled these results, with clavulanate, but not sulbactam or tazobactam, antagonizing the activity of several beta-lactams aga ... | 1990 | 2327752 |
| molecular epidemiology of tem-3 (ctx-1) beta-lactamase. | a total of 33 clinical isolates encoding tem-3 (ctx-1) from four french hospitals were studied. the strains belonged to seven species, klebsiella pneumoniae (n = 24), escherichia coli (n = 3), serratia marcescens (n = 2), citrobacter freundii (n = 1), enterobacter aerogenes (n = 1), enterobacter cloacae (n = 1), and klebsiella oxytoca (n = 1). all the strains harbored an inc7 or m self-transferable plasmid with a size of approximately 85 kilobases. the plasmids had closely related ecori, hincii, ... | 1990 | 2327769 |
| in vitro antibacterial activities of three plants used in traditional medicine in sierra leone. | the antibacterial activities of the methanol and hot and cold aqueous extracts of the leaves of aspilia africana, ficus exasperata and mareya micrantha were bioassayed against three gram-negative and three gram-positive bacterial species: aerobacter aerogenes, agrobacterium tumefaciens, bacillus subtilis, clostridium sporogenes, escherichia coli and staphylococcus aureus. the methanol and aqueous extracts of the leaves of aspilia africana and mareya micrantha and the undiluted oil of m. micranth ... | 1990 | 2335960 |
| comparison of enrichment and plating media for isolation of yersinia. | yersinia enterocolitica (serotypes 0:3 or 0:8), yersinia frederiksenii, yersinia kristensenii, or yersinia intermedia along with 10(8) cells of each of three extraneous organisms (escherichia coli, enterobacter aerogenes, pseudomonas fragi), all commonly found on market poultry, were inoculated into five enrichment media followed by streaking onto 11 plating media to determine the most-efficacious combinations for future surveys or assessment studies. for yersinia enterocolitica (0:8), infrequen ... | 1990 | 2356183 |
| the impact of pulmonary infections on the survival of lung cancer patients. | to determine the impact of pulmonary infections on survival in patients with lung cancer, a retrospective review of the records of 121 such patients treated at howard university hospital in washington, dc, was done. there were 77 men and 44 women; 118 were black. the mean age was 63.5 years. forty-three patients had squamous cell carcinoma, 31 had adenocarcinoma, 18 had large cell carcinoma, 19 had small cell carcinoma, and ten were unclassified. the stages were as follows: two patients in stage ... | 1990 | 2364370 |
| antimicrobial effect of chlorhexidine in a controlled release delivery system. | chlorhexidine is widely used as a mouth rinse in the prevention and treatment of periodontal diseases and dental caries. the purpose of the present study was to evaluate the in vitro antimicrobial effect of chlorhexidine in a controlled release delivery system. the controlled release dispenser comprised a polymeric inner core matrix containing the medicament with an outer vinyl membrane controlling the drug release. the effect on the following bacteria was studied: actinobacillus actinomycetemco ... | 1990 | 2390965 |
| crossover assessment of serum bactericidal activity and pharmacokinetics of five broad-spectrum cephalosporins in the elderly. | to better define the pharmacokinetics and serum bactericidal activity (sba) of the expanded-spectrum cephalosporins in the elderly, we administered single 2-g intravenous infusions of cefoperazone, cefotaxime, ceftriaxone, ceftazidime, and ceftizoxime to six healthy volunteers over the age of 65 years. serum was collected over 24 h, and concentrations were determined by high-performance liquid chromatography; pharmacokinetic parameters were determined for each drug. sba was measured against repr ... | 1990 | 2393258 |
| l-2,4-diaminobutyric acid decarboxylase activity responsible for the formation of 1,3-diaminopropane in enterobacter aerogenes. | high content of 1,3-diaminopropane (dap), a normally minor derivative of polyamine metabolism, have been observed in cells of enterobacter aerogenes. supplementation of the growth medium with l-2,4-diaminobutyric acid (l-daba) resulted in increased production of dap, but not if supplemented with spermidine. on the basis of these observations, the biosynthetic route for dap was evaluated. it has appeared that this bacterium possesses a novel enzyme activity catalysing the formation of dap from l- ... | 1990 | 2397881 |
| cloning and nucleotide sequence of the enterobacter aerogenes signal peptidase ii (lsp) gene. | in escherichia coli, prolipoprotein signal peptidase is encoded by the lsp gene, which is organized into an operon consisting of iles, lsp, and three open reading frames, designated genes x, orf-149, and orf-316. the enterobacter aerogenes lsp gene was cloned and expressed in e. coli. the nucleotide sequence of the enterobacter aerogenes lsp gene and a part of its flanking sequences were determined. a high degree of homology was found between the e. coli iles-lsp operon and the corresponding gen ... | 1990 | 2403548 |
| role of the nac gene product in the nitrogen regulation of some ntr-regulated operons of klebsiella aerogenes. | a positive, genetic selection against the activity of the nitrogen regulatory (ntr) system was used to isolate insertion mutations affecting nitrogen regulation in klebsiella aerogenes. two classes of mutation were obtained: those affecting the ntr system itself and leading to the loss of almost all nitrogen regulation, and those affecting the nac locus and leading to a loss of nitrogen regulation of a family of nitrogen-regulated enzymes. the set of these nac-dependent enzymes included histidas ... | 1990 | 1979323 |
| studies on lysine:n6-hydroxylation by cell-free systems of aerobacter aerogenes 62-1. | electron microscopic examination has revealed the vesicular nature of the membrane component, of the cell-free system of aerobacter aerogenes 62-1, which catalyses lysine: n6-hydroxylation. regardless of the orientation of the vesicles, n-hydroxylation process is still stimulated by pyruvate. both pyruvate oxidation and lysine: n6-hydroxylation were inhibited by protonophores and gramicidin s. | 1989 | 2493814 |
| evolution of chemotactic-signal transducers in enteric bacteria. | the methyl-accepting chemotactic-signal transducers of the enteric bacteria are transmembrane proteins that consist of a periplasmic receptor domain and a cytoplasmic signaling domain. to study their evolution, transducer genes from enterobacter aerogenes and klebsiella pneumoniae were compared with transducer genes from escherichia coli and salmonella typhimurium. there are at least two functional transducer genes in the nonmotile species k. pneumoniae, one of which complements the defect in se ... | 1989 | 2496104 |
| bacterial colonization of intravenous catheter materials in vitro and in vivo. | four different intravenous catheter materials, brands teflon, silastic, vialon, and tecoflex, were evaluated in vitro for bacterial adherence after 2 and 24 hours' incubation in trypticase soy broth and after 2 hours' incubation in nutrient-free phosphate buffer (ph 7.2). the organisms used were pseudomonas aeruginosa, enterobacter aerogenes, escherichia coli, and staphylococcus epidermidis. the significant differences in in vitro adherence of the different bacterial species to the various cathe ... | 1989 | 2500724 |
| microbiological quality and public health significance of ice-cream. | fifty samples of ice-cream were subjected to microbiological examination. of these 72% had total viable count over 10(6)/g while 66% had coliform count between 10(2)-10(3)/g. the micro-organisms isolated were escherichia coli (46%), enterobacter aerogenes (34%), staphylococcus aureus (26%), proteus species (16%), streptococcus faecalis (12%), citrobacter species (10%) and bacillus cereus (4%). the results showed that commercially prepared ice-cream sold in the market was not satisfactory for hum ... | 1989 | 2501524 |
| urinary tract infection. | over two years, 9892 mid-stream urine samples from patients attending the aga khan university hospital, karachi were cultured. significant bacterial growth was seen in 23.5% samples. further identification of these organisms revealed 40% of e. coli, 16% pseudomonas aeruginosa, 11% klebsiella aerogenes, 5.0% enterobacter sp., 13% proteus sp., 4.0% serratia liquifaciens, 1.0% acinetobacter sp., 3.0% citrobacter sp., 4.0% enterococci, 0.5% staphylococcus aureus. results of sensitivity tests perform ... | 1989 | 2504948 |
| [susceptibility of clinical isolates to aztreonam]. | in vitro antibacterial activities of 9 antibiotics including aztreonam (azt) against clinically isolated gram-negative bacteria were determined using mic-2000 plus system. bacteria were isolated from clinical materials in saga medical school during a period from may 1987 to march 1988. summarized results were as follows: 1. azt showed excellent antibacterial activities against escherichia coli, klebsiella pneumoniae, proteus sp. and haemophilus influenzae, and mic80 values of azt against these o ... | 1989 | 2507799 |
| immunoprotective extracellular polysaccharides of klebsiella aerogenes capsular type k1, expressed in escherichia coli. | a gene library of genomic dna klebsiella aerogenes of capsular serotype k1 was constructed in e. coli le392 using the cosmid pmmb33. culture filtrates of e. coli recombinants were screened by elisa for extracellular polysaccharides specific for k. aerogenes k1. extracellular polysaccharide extracts from k. aerogenes k1 and 3% of the e. coli recombinants contained immunoprotective extracellular polysaccharides (iep) with similar chemical and immunological properties as shown by gel filtration thr ... | 1989 | 2517220 |
| human placental 17 beta-hydroxysteroid dehydrogenase is homologous to nodg protein of rhizobium meliloti. | the amino acid sequence of human placental 17 beta-hydroxysteroid dehydrogenase (17 beta-oh-steroid dehydrogenase) was found to be similar to that of the nodg protein of rhizobium meliloti. the computer-based comparison score is 11.5 sd higher than that obtained with 2500 comparisons of randomized sequences of these proteins. the probability of getting such a score by chance is 6 x 10(-31). 17 beta-oh-steroid dehydrogenase is also similar to klebsiella aerogenes ribitol dehydrogenase and escheri ... | 1989 | 2547159 |
| isolation and identification of 6-desmethylnaproxen sulfate as a new metabolite of naproxen in human plasma. | a new metabolite of naproxen, 6-desmethylnaproxen sulfate (6-dmns), has been identified in plasma from normal and uremic subjects after oral administration of a single 500 mg dose of naproxen. tentative identification was achieved by the finding of an increase in the concentration of 6-dmn upon incubation of the plasma with arylsulfatase from helix pomatia or from aerobacter aerogenes. more definitive identification was established through demonstration that the hplc retention time of the conjug ... | 1989 | 2566468 |
| survival of and plasmid stability in pseudomonas and klebsiella spp. introduced into agricultural drainage water. | cell survival and plasmid stability in pseudomonas fluorescens r2f and pseudomonas putida cym 318 containing respectively, plasmid rp4 and prk2501, and klebsiella aerogenes nctc 418 harboring plasmid pbr322 were studied in sterile and nonsterile agricultural drainage water under both aerobic and anaerobic conditions and in the absence and presence of added nutrients. both pseudomonas strains survived well in sterile drainage water incubated aerobically, with or without added nutrients. however, ... | 1989 | 2590305 |
| in-vitro activity of cefetamet (ro 15-8074) compared with other oral agents. | the bacteriostatic and bactericidal activities of cefetamet (ro 15-8074) were compared with those of cefadroxil, cefaclor, ampicillin, doxycycline, chloramphenicol and erythromycin against a total of 400 bacterial strains isolated from nosocomial respiratory tract and auditory canal infections. the broth macrodilution method was used to determine antimicrobial susceptibility. none of the compounds was effective against pseudomonas aeruginosa and methicillin-resistant staphylococcus aureus strain ... | 1989 | 2613603 |
| hydrogen evolution of enterobacter aerogenes depending on culture ph: mechanism of hydrogen evolution from nadh by means of membrane-bound hydrogenase. | the ph dependency of cell mass productivity, the hydrogen evolution rate and the yield of hydrogen from glucose was measured by controlling the ph of the culture automatically. the cell mass productivity of enterobacter aerogenes increased in a linear fashion up to a ph value of approx. 7.0. in contrast, both the evolution rate and the yield of hydrogen showed convex relationships up to a ph value of 7.0, both having maximum values at a ph of approx. 5.8. the maximum evolution rate was approx. 1 ... | 1989 | 2643990 |
| detection of antigens from gram-negative bacilli in urine of children with urinary tract infections. ii. thermostable bacterial proteins. | an enzyme immunoassay for detection of bacterial antigens in urine specimens from urinary tract infections (uti) was developed. the antigens detected in infections by enterobacteriaceae: escherichia coli, enterobacter aerogenes, and proteus sp. were different from the enterobacterial common antigen (eca). they were digested by trypsin and were characterized by remarkable thermostability; their molecular weights were approximately 34,000 and 31,000. they were detected by means of antisera to eca ... | 1989 | 2656535 |
| microcalorimetric studies of the effects of platinum group metal complexes on bacterial growth. | a range of platinum group metal complexes (pgmc) were screened for antibacterial activity against klebsiella aerogenes and staphylococcus aureus. the effects of the complexes were monitored by changes in the thermal and growth properties of the organisms. active complexes caused an immediate cessation of power and biomass production which did not recover whilst the complex was present in the medium. removal of the complex by washing the cells allowed the growth of the small viable proportion of ... | 1989 | 2661974 |
| [clinical studies on sulbactam/ampicillin in the field of pediatrics]. | during 8 months from october 1986 to may 1987, the clinical efficacy of sulbactam/ampicillin (sbt/abpc) was evaluated in 63 pediatric inpatients with various infections. clinical efficacies were evaluable in 58 patients among them (consisting of 2 patients with sepsis, 3 with tonsillitis, 12 with bronchitis, 6 with bronchopneumonia, 24 with pneumonia, 1 with phlegmon, 2 with lymphadenitis, 1 with impetigo and 7 with urinary tract infection) and were excellent in 40 patients and good in 17 with a ... | 1989 | 2664249 |
| [susceptibilities of clinical isolates to antibacterial agents. a study mainly focused on ofloxacin (the second report). reported by the research group for testing ofloxacin susceptibility on clinical isolates]. | susceptibilities of various clinical isolates to ofloxacin (oflx) and other antibacterial drugs were examined at 128 hospital laboratories in 36 prefectures throughout japan between april, 1986 and march, 1987. the results were totalized with an emphasis mainly on oflx and were compared with data obtained in the previous year. in this study, identification and susceptibility tests of the isolates were carried out at each hospital laboratory and the tests were performed according to the 1-dilutio ... | 1989 | 2664255 |
| "spontaneous" pneumocephalus associated with aerobic bacteremia. | three cases of "spontaneous" pneumocephalus suspected to have resulted from aerobic bacteremia caused by enterobacter cloacae, escherichia coli, and klebsiella aerogenes are reported. in two cases, the e. cloacae and k. aerogenes were isolated from the cerebrospinal fluid. these cases were characterized by a rapid accumulation of air, without niveau, in the subarachnoid space and ventricles. | 1989 | 2670144 |
| prevention of clinical coliform mastitis in dairy cows by a mutant escherichia coli vaccine. | a prospective cohort study was undertaken in two commercial california dairies. the treatment group, 246 cows, received three doses of a whole cell bacterin of j5 escherichia coli (mutant of e. coli o111:b4) plus freund's incomplete adjuvant vaccine (two in the dry period and one after calving) while 240 unvaccinated cows served as controls. thirty-five cases of clinical coliform mastitis were diagnosed, six in vaccinated cows and 29 in unvaccinated cows. bacteria isolated from the clinical case ... | 1989 | 2670166 |
| isolation and characterization of an alginate lyase from klebsiella aerogenes. | the bacterium klebsiella aerogenes (type 25) produced an inducible alginate lyase, whose major activity was located intracellularly during all growth phases. the enzyme was purified from the soluble fraction of sonicated cells by ammonium sulfate precipitation, anion- and cation-exchange chromatography and gel filtration. the apparent molecular weight of purified alginate lyase of 28,000 determined by gel filtration and of 31,600 determined by sodium dodecyl sulfate-polyacrylamide gel electropho ... | 1989 | 2673122 |
| competitive inhibitors of klebsiella aerogenes urease. mechanisms of interaction with the nickel active site. | we examined several compounds for their mechanisms of inhibition with the nickel-containing active site of homogeneous klebsiella aerogenes urease. thiolate anions competitively inhibit urease and directly interact with the metallocenter, as shown by the ph dependence of inhibition and by uv-visible absorbance spectroscopic studies. cysteamine, which possesses a cationic beta-amino group, exhibited a high affinity for urease (ki = 5 microm), whereas thiolates containing anionic carboxyl groups w ... | 1989 | 2674118 |
| comparison of sequences from the malb regions of salmonella typhimurium and enterobacter aerogenes with escherichia coli k12: a potential new regulatory site in the interoperonic region. | the male and malk genes from salmonella typhimurium, and the malefg operon and a portion of malk from enterobacter aerogenes were cloned and sequenced. plasmid-borne male genes from both species and the malf and malg genes from e. aerogenes were expressed normally in escherichia coli, and their products function in maltose transport. this shows that the malb products from the three species are interchangeable, at least in the combinations tested. the general genetic organization of the malb regi ... | 1989 | 2674653 |
| mutagenic dna repair genes on plasmids from the 'pre-antibiotic era'. | resistance transfer factors are natural conjugative plasmids encoding antibiotic resistance. some also encode mutagenic dna repair genes giving resistance to dna damage and induced mutagenesis. it has been shown that antibiotic resistance has been acquired by recent transposition events; however, we show here that mutagenic repair genes existed much earlier on these types of plasmids. conjugative plasmids from eight incompatibility groups from the murray collection of 'pre-antibiotic era' entero ... | 1989 | 2674658 |
| biosynthesis and secretion of pullulanase, a lipoprotein from klebsiella aerogenes. | we constructed, by site-directed mutagenesis, a mutant pullulanase gene in which the cysteine residue in a pentapeptide sequence, leu16-leu-ser-gly-cys20 within the nh2-terminal region of pullulanase from klebsiella aerogenes, is replaced by serine (ser20). the modification, processing, and subcellular localization of the mutant pullulanase were studied. labeling studies with [3h]palmitate and immunoprecipitation with mouse antiserum raised against pullulanase showed that the wild form of both t ... | 1989 | 2677008 |
| preservation of myocardial ultrastructure after 24 hours of klebsiella sepsis: histologic, functional, and biochemical correlations. | myocardial function with ultrastructure and high energy phosphate levels in dogs was correlated after 24 hours of sepsis using live klebsiella aerogenes. all animals developed progressive hemodynamic deterioration over a 24 hour period. mean arterial pressure decreased from 148 +/- 7 mmhg to 85 (p less than 0.01) and cardiac output decreased from 3.43 +/- .31 to 1.6 +/- 0.5 l/min. left ventricular stroke work decreased from 48.2 +/- 5 to 18.1 +/- 6 gm-meters (p less than 0.001). systemic and pul ... | 1989 | 2681798 |
| cadmium resistance in some members of enterobacteriaceae. | strains of members of enterobacteriaceae, namely escherichia coli (18), klebsiella aerogenes (16), and serratia marcescens (16) were screened for cd resistance or sensitivity. only one strain each of these was resistant to high levels (25 n moles/0.05 ml) cdcl2. the minimal inhibitory concentration (mic) of sensitive strains ranged from 0.8-5 micrograms/ml. all the resistant strains were simultaneously resistant to a number of antibiotics. treatment with sodium dodecyl sulfate eliminated resista ... | 1989 | 2684849 |
| ammonia-induced injury in pure cultures and natural populations of coliform bacteria. | ammonia-induced injury was investigated in pure cultures of escherichia coli and enterobacter aerogenes, and in natural coliform populations obtained from the oligotrophic luxapallila and the eutrophic sunflower rivers in northern mississippi. pure cultures were affected by ammonia exposure as indicated by changes in the injury ratio (ir) of cfu on m-t7 agar/cfu on m-endo agar. ammonia concentrations between 0 and 20 (mg nh3-n/1) had little or no effect and concentrations between 40 and 80 cause ... | 1989 | 2693200 |
| regulation of gene expression and cellular localization of cloned klebsiella aerogenes (k. pneumoniae) urease. | the genes for klebsiella aerogenes (k. pneumoniae) urease were cloned and the protein was overexpressed (up to 18% of total protein consisted of this enzyme) in several hosts. the small size of the dna encoding urease (3.5 kb), the restriction map, and the regulation of enzyme expression directed by the recombinant plasmid are distinct from other cloned ureases. nickel concentration did not affect urease gene expression, as demonstrated by the high levels of apoenzyme measured in cells grown in ... | 1989 | 2693604 |
| epidemiological typing of enterobacter aerogenes. | the applicability of enterobacter cloacae and klebsiella typing reagents for classifying clinical strains of enterobacter aerogenes was evaluated. of 75 strains, none were agglutinated by e. cloacae o antisera or were sensitive to e. cloacae bacteriophages. in contrast, 70 strains reacted with klebsiella capsular antisera. two-thirds of the strains were lysed by klebsiella typing phages. a set of five e. aerogenes bacteriocin producers classified 92% of strains into 15 sensitivity types. in conc ... | 1989 | 2715326 |
| comparative activity of newer antibiotics against gram-negative bacilli. | the in vitro activities of cefoperazone, cefotaxime, ceftriaxone, ceftazidime, azlocillin, mezlocillin, piperacillin, ticarcillin/clavulanate, aztreonam, imipenem, and ciprofloxacin were concurrently determined against over 1,000 isolates of gram-negative bacilli from clinical specimens of patients at the cleveland clinic. cephalosporins, penicillins, and aztreonam were active against species of enterobacteriaceae other than citrobacter freundii, enterobacter aerogenes, and enterobacter cloacae. ... | 1989 | 2731335 |
| formation of tyrosine o-sulfate by mitochondria and chloroplasts of euglena. | mitochondria that have been purified from cells of light-grown wild-type euglena gracilis klebs var. bacillaris cori or dark-grown mutant w10bsml and incubated with 35so4(2-) and atp accumulate a labeled compound in the surrounding medium. this compound is also labeled when mitochondria are incubated with [14c]tyrosine and nonradioactive sulfate under the same conditions. this compound shows exact coelectrophoresis with synthetic tyrosine o-sulfate at ph 2.0, 5.8, and 8.0, and yields sulfate and ... | 1989 | 2735764 |
| comparative in vitro activity of ciprofloxacin and other unrelated antimicrobials against bacterial respiratory tract pathogens. | ciprofloxacin is a new fluorinated 4-quinolone with a broad spectrum of antimicrobial activity which includes both gram-negative and gram-positive bacteria. in this study the in vitro activity of ciprofloxacin has been determined against bacteria associated with respiratory tract infections and compared with that of other antimicrobial agents used in the therapy of such infections. ciprofloxacin (mic90 0.008 mg/l) was highly active against haemophilus influenzae, including isolates producing bet ... | 1989 | 2737080 |
| a microbial carbon-phosphorus bond cleavage enzyme requires two protein components for activity. | enterobacter aerogenes ifo 12010 contains a carbon-phosphorus (c-p) bond cleavage enzyme catalyzing the liberation of inorganic phosphate from various alkyl- and phenylphosphonic acids. the enzyme in the bacterium was found to be composed of two physically different protein components, e2 and e3. the molecular weights of e2 and e3 were 560,000 and 110,000, respectively, and e3 was resolved into two apparently homogeneous subunits. neither component alone could catalyze the c-p bond cleavage reac ... | 1989 | 2753863 |
| isolation and survival of gentamicin resistant enterobacter aerogenes on finger tips of hospital personnel. | enterobacter aerogenes was isolated from the finger tips of 13.3 percent of hospital personnel while they were working in the wards. gentamicin resistant strains were isolated more frequently than gentamicin sensitive. carriage rate of e. aerogenes was higher among nurses than other staff members. there was no correlation between antibiotic resistance, capsular serotypes and survival of these strains on finger tips. the property of prolonged survival on finger tips is chromosomal in nature and n ... | 1989 | 2757748 |
| [meconium peritonitis complicated with enterobacter aerogenes infection--a case report]. | meconium peritonitis is an aseptic peritonitis that follows a perforation of the intestines in fetal life. it is a serious complication of intestinal perforation. this article reports a female newborn who was born with gestational age of 40 weeks and birth body weight of 3400 gm. she was admitted at age of 6 days, owing to persistent vomiting, abdominal distention, tachypnea and acrocyanosis since birth. the plain abdomen showed intra-abdominal calcification and intestinal distention. under the ... | 1989 | 2766063 |
| high efficiency transduction of single strand plasmid dna into enteric bacteria. | this report demonstrates high efficiency transduction of enteric bacteria using single strand plasmids packaged in m13 phage capsids. transformation by plasmid dna is usually a very inefficient process in many enteric bacteria other than escherichia coli k12. plasmids carrying an m13 origin of replication can be replicated and packaged when cells carrying such plasmids are infected with m13 or a derivative helper phage. by introducing an f' plasmid into e. coli, serratia marcescens, citrobacter ... | 1989 | 2779519 |
| origin of p-aminobenzoic acid from chorismic rather than iso-chorismic acid in enterobacter aerogenes and streptomyces species. | enzyme extracts from enterobacter aerogenes (62-1), streptomyces aminophilus, and streptomyces coelicolor were used to investigate the biosynthesis of p-aminobenzoic acid. the enzyme preparations from e. aerogenes and s. aminophilus contained both p-aminobenzoate synthase and iso-chorismate synthase activity, and were able to convert both chorismic and iso-chorismic acid to p-aminobenzoic acid. the apparent km for chorismic acid was, however, significantly lower than that for iso-chorismic acid, ... | 1989 | 2786373 |
| lipid a precursor from pseudomonas aeruginosa is completely acylated prior to addition of 3-deoxy-d-manno-octulosonate. | inhibition of lipopolysaccharide (lps) synthesis in pseudomonas aeruginosa at the stage of incorporation of 3-deoxy-d-manno-octulosonate (kdo) caused accumulation of a lipid a precursor which contained all of the fatty acids present on the lipid a of mature lps. the enzyme ctp:cmp-3-deoxy-d-manno-octulosonate cytidylyltransferase (cmp-kdo synthetase) from p. aeruginosa is inhibited by the kdo analog alpha-c-[1,5-anhydro-8-amino-2,7,8-trideoxy-d-manno-octopyranosyl] carboxylate (i), and i is effe ... | 1988 | 2833499 |
| bidirectional promoter in the hut(p) region of the histidine utilization (hut) operons from klebsiella aerogenes. | the hut(p) region (i.e., the region responsible for regulation of hutuh expression) of the klebsiella aerogenes histidine utilization (hut) operons contains a bidirectional promoter. one transcript from this promoter encodes the hutuh operon; the role of the oppositely directed transcript is unknown, although it appears to be involved in regulating hutuh expression (a.j. nieuwkoop, s.a. boylan, and r.a. bender, j. bacteriol. 159:934-939, 1984). a 247-base-pair (bp) fragment containing hut(p) car ... | 1988 | 2834335 |
| contact alters camp metabolism in aggregation-competent dictyostelium amoebae. | camp and cell-cell contact are involved in the coordination of differentiation and morphogenesis in dictyostelium discoideum. the experiments described in this paper establish a relationship between camp and cell-cell contact. contact between enterobacter aerogenes and aggregation-competent dictyostelium amoebae and contact between dictyostelium amoebae themselves results in the transient secretion of camp and an alteration in the amount of camp secreted in response to subsequent stimulation by ... | 1988 | 2854020 |
| plasmid-mediated conjugative transfer of klebsiella sp. rcs genesable to induce colanic acid capsular polysaccharide biosynthesis in escherichia coli. | regulation of capsular biosynthesis (rcs) genes, encoding the ability to induce the production of a colanic acid polysaccharide capsule, were transferred to escherichia coli by conjugation with klebsiella pneumoniae (aerogenes) of capsular serotype k36. transfer was mediated by a 58.4-mda conjugative plasmid of incompatibility group incm, which carried a copy of tn7 (specifying resistance to trimethoprim and streptomycin) together with determinants for several further resistances. this plasmid d ... | 1988 | 2856173 |
| effect of macromolecular composition of microorganisms on the thermodynamic description of their growth. | most models describing bacterial growth, including the original mosaic non-equilibrium thermodynamic (mnet) description, do not take into account that the macromolecular composition of the cells varies with growth rate. the mnet description of bacterial growth is extended to account for such a variation in macromolecular composition of the cells in order to make the mnet description more generally applicable. klebsiella aerogenes nctc 418 was cultured in a chemostat under glucose- or ammonia-lim ... | 1988 | 2461738 |
| a laboratory model for testing agents for hygienic hand disinfection: handwashing and chlorhexidine for the removal of klebsiella. | a model for the laboratory assessment of hygienic hand disinfection is described which closely reproduces handwashing in a ward setting. the model uses a clinically relevant test organism and takes into account the natural antibacterial action of the skin. it also provides a means of measuring any residual antibacterial action after hand disinfection. using an epidemic multiply-resistant strain of klebsiella aerogenes k21, 0.5% chlorhexidine in iso-propyl alcohol yielded sterile post-disinfectio ... | 1988 | 2904456 |
| [use of imipenem-cilastatin in neonatal septicemias caused by gram-negative bacilli multiresistant to beta-lactam antibiotics]. | seven neonates with septicemia due to gram negative bacteria resistant to beta-lactam received imipenem-cilastatin therapy. bacteria isolated were enterobacter cloacae [3], enterobacter aerogenes [1], klebsiella pneumoniae [1], serratia marcescens [1], pseudomonas fluorescens [1]. the mics of imipenem were lower 1 microgram/ml. in 3 children septicemia occurred during previous antimicrobial chemotherapy. 3 iv 60 mg/kg doses of imipenem with amikacin (15 mg kg/d) were administered every day. for ... | 1988 | 3043351 |
| a bacterial factor induces changes in cysteine proteinase forms in the cellular slime mould dictyostelium discoideum. | the electrophoretic pattern of cysteine proteinases in axenically grown myxamoebae of dictyostelium discoideum can be altered by the addition of either gram-negative (klebsiella aerogenes, escherichia coli) or gram-positive (micrococcus lysodeikticus, bacillus subtilis) bacteria to the culture. no changes occurred, however, if either yeast or latex beads were used in place of bacteria. the changes involved the simultaneous loss of proteinases characteristic of the axenic cells (the a-forms) and ... | 1988 | 3052431 |
| divergence of the aerobactin iron uptake systems encoded by plasmids pcolv-k30 in escherichia coli k-12 and psmn1 in aerobacter aerogenes 62-1. | although the aerobactin-mediated iron uptake system has been characterized genetically in escherichia coli, the siderophore aerobactin was chemically characterized after purification from culture supernatants of aerobacter aerogenes 62-1, a member of the klebsielleae. we have cloned and mapped the genes encoding the aerobactin system genes of a. aerogenes 62-1 and begun characterization of the relevant proteins and enzymatic activities of this plasmid-mediated aerobactin system. published chemic ... | 1988 | 3053646 |
| evolution of aminobenzoate synthases: nucleotide sequences of salmonella typhimurium and klebsiella aerogenes pabb. | p-aminobenzoate synthase (ps) and anthranilate synthase (as) are structurally related enzymes that catalyze similar reactions and produce similar products, para- and ortho-aminobenzoate (anthranilate). each enzyme is composed of two non-identical subunits: a glutamine amidotransferase subunit (coii) and a subunit that produces an aminobenzoate product (coi). nucleotide sequence comparisons of the escherichia coli genes encoding each of the subunits suggest a common evolutionary origin for both s ... | 1988 | 3057324 |
| effect of subinhibitory concentration of gentamicin on adherence of klebsiella aerogenes to uroepithelial cells in vitro. | 1988 | 3058587 | |
| comparison of broth enrichment and direct plating for the isolation of campylobacter jejuni from dogs. | two techniques for the isolation of campylobacter jejuni from feces, direct plating and thioglycolate broth enrichment, were compared. a total of 272 rectal swab cultures were performed on 156 laboratory dogs. campylobacter blood agar plates and campylobacter thioglycolate broth were inoculated immediately upon sampling of the dogs. after incubation at 4 degrees c for 12 to 16 h, material from the campylobacter thioglycolate medium was inoculated onto campylobacter blood agar plates. a total of ... | 1988 | 3069857 |
| [a specific property of bacteria in the genus klebsiella--a color reaction with 5-aminosalicylic acid in the nutrient medium]. | for the first time bacteria of the genus klebsiella have been found to possess a specific property, characteristic of this genus only, i.e. the capacity of giving color reaction with 5-aminosalicylic acid. this reaction can be observed in all klebsiella species under study: k. pneumoniae (94.4 +/- 2.3%), k. ozaenae (93.3 +/- 4.5%), k. rhinoscleromatis (100%), k. oxytoca (88.0 +/- 4.9%), k. mobilis (92-5 +/- 4.3% of the strains). in all other bacteria under study (40 species, 22 genera and 7 fami ... | 1988 | 3072806 |
| [biochemical typing of enterobacter isolated from several clinical materials]. | a total of 74 enterobacter species have been isolated from the patients applying to the department of microbiology, university of ankara, studying several biochemical test systems their strains have been found. 37 of these enterobacter species have been found to be enterobacter cloacae, 10 enterobacter agglomerans, 13 enterobacter aerogenes, 3 enterobacter hafniae (hafnia alvei), 1 enterobacter sakazakii. 2 of the strains couldn't be classified. in conclusion most of the strains were found to be ... | 1988 | 3078792 |
| bacterial adhesion to intravenous cannulae: influence of implantation in the rabbit and of enzyme treatments. | comparison was made of the adhesion of staphylococcus epidermidis, staphylococcus aureus, escherichia coli, klebsiella aerogenes and pseudomonas aeruginosa to six types of intravascular cannula material. adhesion to materials removed from rabbit tissues did not differ significantly between types of material or between bacterial species. in contrast, major differences were found when unimplanted materials were examined; the overall rank order of adhesiveness of bacteria to unimplanted materials ( ... | 1988 | 3123262 |
| [pseudomonas aeruginosa and surgical intensive care units]. | the prevalence of pseudomonas aeruginosa in intensive care unit (icu) is 19 per cent although its incidence in blood culture is only 3.5 per cent and remains the same even though the total prevalence of this bacterium increases. a review of the clinical results shows that this germ causes severe complications in only 3 per cent of patients. a study by epidemiological markers reveals the presence of 16 different iats serotypes in the icu, this distribution of the serotypes being similar to this o ... | 1988 | 3132871 |
| in vitro evaluation of e1040, a new cephalosporin with potent antipseudomonal activity. | e1040 is a new parenteral cephalosporin with a broad antibacterial spectrum and potent antipseudomonal activity. the compound was four- to eightfold more active than ceftazidime and cefsulodin against pseudomonas aeruginosa (mic of e1040 for 90% of strains tested [mic90], 3.13 micrograms/ml). e1040 also showed a potent activity against other glucose-nonfermentative rods, including acinetobacter species. the activities of e1040 against most species of the family enterobacteriaceae were roughly co ... | 1988 | 3134847 |