Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
descriptive epidemiology of vaginal cancer incidence and survival by race, ethnicity, and age in the united states.vaginal cancer is a rare malignancy. it has many of the same risk factors as cervical cancer, including a strong association with persistent human papillomavirus infection. descriptive studies of the epidemiology of vaginal cancer are scarce in the literature.200818980291
[item no 149: epithelial and melanoma skin tumors: tumors due to human papillomavirus]. 200818984204
public knowledge and attitudes towards human papilloma virus (hpv) vaccination.human papilloma virus (hpv) vaccine has undergone successful trials and has recently been approved for use for the primary prevention of cervical cancer. the aim of this study was to determine knowledge and attitudes towards hpv vaccination.200818947430
kinase requirements in human cells: ii. genetic interaction screens identify kinase requirements following hpv16 e7 expression in cancer cells.human papillomavirus (hpv) oncoproteins subvert cellular signaling pathways, including kinase pathways, during the carcinogenic process. to identify kinases targeted by the hpv16 e7 oncoprotein, shrna kinase screens were performed in rko colorectal carcinoma cell lines that differ only in their expression of hpv16 e7. our screens identified kinases that were essential for the survival of rko cells, but not essential for rko cells expressing hpv16 e7. these kinases include cdk6, erbb3, fyn, aak1, ...200818948598
human papillomavirus in the placenta and umbilical cord blood.to analyze human papillomavirus (hpv) dna in umbilical cord blood and in placenta, including its cellular localization.200818972230
national ambulatory medical care survey: 2006 summary.this report describes ambulatory care visits made to physician offices in the united states. statistics are presented on selected characteristics of the physician's practice, the patient, and the visit.200818972720
prevalence, acquisition, and clearance of cervical human papillomavirus infection among women with normal cytology: hawaii human papillomavirus cohort study.few natural history studies of cervical human papillomavirus (hpv) incidence and duration have been conducted among older women, especially from multiethnic populations. viral and nonviral determinants of hpv acquisition and clearance were examined among 972 sexually active women, ages 18 to 85 years, recruited from clinics on oahu, hawaii, and followed for a mean duration of 15 months (range, 2-56 months). interviews and cervical cell specimens for cytology and hpv dna detection by pcr, using t ...200818974124
new approaches to cervical cancer screening in latin america and the caribbean.cervical cancer remains an important public health problem in the latin america and caribbean region (lac), with an expected significant increase in disease burden in the next decades as a result of population ageing. prophylactic human papillomavirus (hpv) vaccine is currently unaffordable in lac countries. however, even if vaccination was implemented, an additional two decades will be required to observe its impact on hpv related disease and cancer. with some exceptions, cytology-based screeni ...200818945402
recommendations for cervical cancer prevention in latin america and the caribbean.cervical cancer control in the latin america and caribbean (lac) region has been, and remains, a priority and a major public health challenge. it also provides the opportunity for the advancement of research into novel cervical cancer preventative tools including the use of prophylactic human papillomavirus (hpv) vaccines, hpv-based screening options and low technology visual inspection methods. the challenges for prevention are compounded because cervical cancer cases continue to cluster in the ...200818945407
burden and trends of type-specific human papillomavirus infections and related diseases in the asia pacific region.we present the burden of human papillomavirus (hpv)-related cancers (cancers of the cervix, anogenital areas, and oral cavity and pharynx) in terms of incidence and mortality, for the countries of the asia pacific region. the region contains more than half of the world population, and manifests a wide geographic diversity in the prevalence of infection with hpv, and of incidence (and mortality) rates of cancer of the cervix. in general, rates of cancer of the cervix have declined since the 1960' ...200818945410
human papillomavirus infection and cervical cancer prevention in japan and korea.cervical cancer is a common cancer among women in japan and korea. implementation of national cervical cancer screening programs has led to a reduction in the incidence of cervical cancer in both countries. however, over the past decade, there has been a recent marked increase in cervical cancer incidence among young women in japan. human papillomavirus (hpv) is found in the majority of cervical cancers, and hpv-16 and 18 are the two most common types. the next most frequent hpv types in cervica ...200818945412
human papillomavirus infection and cervical cancer prevention in india, bangladesh, sri lanka and nepal.although one-third of the world cervical cancer burden is endured in india, bangladesh, nepal and sri lanka, there are important gaps in our knowledge of the distribution and determinants of the disease in addition to inadequate investments in screening, diagnosis and treatment in these countries. prevalence of human papillomavirus (hpv) infection among the general populations varies from 7-14% and the age-specific prevalence across age groups is constant with no clear peak in young women. this ...200818945413
recommendations for cervical cancer prevention in asia pacific.asia oceania includes countries from both the asia pacific region and australasia, which cover very diverse geographical areas and populations as well as bearing 52% of the cervical cancer burden in the world. human papillomavirus (hpv) genotype distribution in women with normal cytology varies between countries in this region, as well as with the distribution typically observed in worldwide estimates or in western countries. hpv-16 remains the predominant oncogenic type for high-grade cervical ...200818945418
hpv vaccines: are they the answer?the burden of human papillomavirus (hpv)-associated ano-genital disease is significant but the ability to generate hpv virus-like particles by the synthesis and self-assembly in vitro of the major virus coat protein l1 has transformed our prospects for preventing benign and malignant ano-genital disease caused by the common genital hpv types.200818940889
hiv, human papillomavirus, and cervical neoplasia and cancer in the era of highly active antiretroviral therapy.the objective of this study was to review the literature on the epidemiological association between human papillomavirus (hpv), hiv, and cervical neoplasia, and the impact of highly active antiretroviral therapy (haart) on this association. medline was searched using the terms 'human papillomavirus', 'hpv', 'hiv', 'cervix', 'neoplasm', and 'antiretroviral' to identify articles published before december 2006. hiv-infection was strongly associated with a higher prevalence, incidence, and persisten ...200818941376
human papillomavirus type spectrum in normal skin of individuals with or without a history of frequent sun exposure.cutaneous human papillomavirus (hpv) has been widely detected in healthy skin. previous studies have found that uv radiation can activate several hpv types, and a possible role for cutaneous hpv in the development of non-melanoma skin cancer has been suggested. this study investigated the prevalence and type-spectrum of cutaneous hpv in relation to uv radiation by studying forehead skin swab samples from 50 healthy males frequently exposed to the sun and 50 healthy males who were not frequently ...200818931088
[hpv infection: comparison between morphological studies and molecular biology].human papilloma virus plays an essential role in the development of cervical cancer. we investigated the global prevalence of human papilloma virus infection in a population of 699 women recruited at the ospedali riuniti in foggia for gynaecological controls from september 2005 to march 2007, and compared with a group of 90 women, selected on clinical aspects for human papilloma virus features. the observed prevalence was 27.4%, which is higher that that reported in the literature. in the study ...200818841817
risk factors for squamous cell carcinoma of the penis--population-based case-control study in denmark.few etiologic studies of squamous cell carcinoma (scc) of the penis have been carried out in populations where childhood circumcision is rare. a total of 71 patients with invasive (n=53) or in situ (n=18) penile scc, 86 prostate cancer controls, and 103 population controls were interviewed in a population-based case-control study in denmark. for 37 penile scc patients, tissue samples were pcr examined for human papillomavirus (hpv) dna. overall, 65% of pcr-examined penile sccs were high-risk hpv ...200818843010
[triage value of high risk human papilloma virus detection in women with abnormal cervical cytology].to evaluate the triage value of high risk human papilloma virus (hrhpv) detection in the women with abnormal cervical cytology.200818844110
human papillomavirus vaccines: an outsider's point of view. 200818844585
analysis of adverse events of potential autoimmune aetiology in a large integrated safety database of as04 adjuvanted vaccines.newly licensed vaccines against human papillomavirus (hpv) and hepatitis b (hbv), and several vaccines in development, including a vaccine against genital herpes simplex virus (hsv), contain a novel adjuvant system, as04, composed of 3-o-desacyl-4' monophosphoryl lipid a and aluminium salts. given the background incidence of autoimmune disorders in some of the groups targeted for immunisation with these vaccines, it is likely that autoimmune events will be reported in temporal association with v ...200818845199
immunobiology of human papillomavirus infection and vaccination - implications for second generation vaccines.prophylactic human papillomavirus (hpv) l1 virus like particle (vlp) vaccines have been shown, in large clinical trials, to be very immunogenic, well-tolerated and highly efficacious against genital disease caused by the vaccine hpv types. however these vaccines, at the present, protect against only two of the 15 oncogenic genital hpv types, they are expensive, delivered by intramuscular injection and require a cold chain. the challenges are to develop cheap, thermo-stable vaccines that can be d ...200818847558
human papillomavirus, cervical cancer prevention, and more. 200818847562
hpv detection rate in saliva may depend on the immune system efficiency.human papilloma virus (hpv) has been established as a major etiological factor of anogenital cancer. in addition, hpv has also been implicated in oral carcinogenesis but its detection rates appear to be highly variable, depending on the patient population tested, the molecular methodology used, as well as the type of oral specimen investigated. for example, saliva is an oral fluid that may play a role in hpv transmission, although the detection rates of the virus are lower than tissue. recent ev ...200818853753
immunogenic properties of recombinant and synthetic peptides of human papillomavirus. 200818853768
cutaneous and mucosal human papillomaviruses differ in net surface charge, potential impact on tropism.papillomaviruses can roughly be divided into two tropism groups, those infecting the skin, including the genus beta pvs, and those infecting the mucosa, predominantly genus alpha pvs. the l1 capsid protein determines the phylogenetic separation between beta types and alpha types and the l1 protein is most probably responsible for the first interaction with the cell surface. virus entry is a known determinant for tissue tropism and to study if interactions of the viral capsid with the cell surfac ...200818854037
controversies in the management of anal high-grade squamous intraepithelial lesions.anal squamous dysplasia is recognized as a spectrum of disease that ranges from low-grade intraepithelial lesions (lsil) to high-grade squamous intraepithelial lesions (hsil) to invasive anal squamous cell carcinoma (scc). recent reports have shown a significant increase in both the incidence and prevalence of both hsil and anal scc, particularly in immunocompromised patients and in men who have sex with men. these lesions are associated with chronic infection with the human papillomavirus. the ...200818923350
cell-cycle and suppressor proteins expression in uterine cervix in hiv/hpv co-infection: comparative study by tissue micro-array (tma).the oncoproteins of human papillomavirus (hpvs) directly effect cell-cycle control. we hypothesize that regulatory and cell cycle protein expression might be additionally modified in the cervix of hiv/hpv co-infected women.200818840277
cellular distribution of tumour suppressor protein p53 and high-risk human papillomavirus (hpv)-18 e6 fusion protein in wild-type p53 cell lines.human papillomaviruses (hpvs) are very important pathogens that can be classified as high- and low-risk types based on the lesions they cause. mucosal high-risk hpv e6 can target and degrade the tumour suppressor p53, hence it is recognized as the major cause of cervical cancer, however, due to a lack of reliable anti-e6 antibodies, the distribution of high-risk hpv e6 protein remains elusive. the present study, therefore, used a mammalian green fluorescent protein (gfp) expression system to exp ...200818831896
[it's only afterwards the medical products agency can scrutinize advertisements]. 200818833633
the new hpv vaccination. 200818834017
proex c immunocytochemistry and high-risk human papillomavirus dna testing in papanicolaou tests with atypical squamous cell (asc-us) cytology: correlation study with histologic biopsy.papanicolaou tests with atypical squamous cells of undetermined significance (asc-us) cytology and adjunct testing for high-risk human papillomaviruses (hr-hpv) are helpful in detecting high-grade disease. detection of disease may be further improved with molecular markers known to be overexpressed in cervical carcinoma. proex c detects 2 such molecular markers, minichromosome maintenance protein 2 and topoisomerase ii, which are associated with abnormal cell cycle regulation.200818834224
clathrin- and caveolin-independent entry of human papillomavirus type 16--involvement of tetraspanin-enriched microdomains (tems).infectious entry of human papillomaviruses into their host cells is an important step in the viral life cycle. for cell binding these viruses use proteoglycans as initial attachment sites. subsequent transfer to a secondary receptor molecule seems to be involved in virus uptake. depending on the papillomavirus subtype, it has been reported that entry occurs by clathrin- or caveolin-mediated mechanisms. regarding human papillomavirus type 16 (hpv16), the primary etiologic agent for development of ...200818836553
topical cidofovir for severe warts in a patient affected by aids and hodgkin's lymphoma.we describe a 42-year-old man with aids and hodgkin's lymphoma whose severe and recalcitrant cutaneous warts resolved following treatment with local 1% cidofovir. clinically significant improvements were observed in a two-week period of therapy. in advanced hiv disease complicated by additional haematological malignancy, cutaneous warts may be difficult to treat and present a challenge for the attending physicians. in similar clinical condition topical anti-human papillomavirus therapy may prove ...200818824628
expression and polimorphism of ifn-gamma gene in patients with cervical cancer.cervical cancer, the second most common malignancy in women worldwide, is almost invariably associated with infection by human papillomavirus (hpv). however, although many women are infected with high-risk types of hpv, only a subset of infected women will ever develop cervical cancer. several studies suggested that immunological components play a key role in the development of cervical cancer. interferon gamma (ifn-gamma ) is a cytokine produced by activated t cells and natural killer (nk) cell ...200818806746
genotyping of human papillomavirus dna in anal biopsies and anal swabs collected from hiv-seropositive men with anal dysplasia.human papillomavirus (hpv) causes anal intraepithelial neoplasia (ain) in hiv-seropositive men. the detection of hpv genotypes in anal biopsies and swabs was compared.200818667921
human papilloma virus-dependent hmga1 expression is a relevant step in cervical carcinogenesis.hmga1 is a member of a small family of architectural transcription factors involved in the coordinate assembly of multiprotein complexes referred to as enhanceosomes. in addition to their role in cell proliferation, differentiation, and development, high-mobility group proteins of the a type (hmga) family members behave as transforming protoncogenes either in vitro or in animal models. recent reports indicated that hmga1 might counteract p53 pathway and provided an interesting hint on the mechan ...200818670638
paediatricians' attitudes and practices towards hpv vaccination.in june 2006, the human papillomavirus (hpv) vaccine, gardasil, was licensed for use in the united states. we examined whether paediatricians would recommend the vaccine, obstacles they encountered and characteristics associated with not recommending the hpv vaccine to all eligible patients.200818671696
human papilloma virus infection and cervical intraepithelial neoplasia in transplanted patients.progress in diagnosis and treatment has led to an increased number of transplantation patients who consequently have immunological depression and emergence of tumors. the incidence of cervical neoplasia, according to previous studies, is 11%; this tumor is the only one that can be investigated by screening before and after a graft. our purpose was to evaluate whether transplanted patients showed an increased incidence of genital human papilloma virus (hpv) infection and whether this infection pr ...200818675077
human papillomavirus vaccination coverage on youtube.a large percentage of internet users regularly search for health-related information. in recent years, participatory internet sites such as youtube have become increasingly popular, in part because individuals are able to both retrieve and post information. this study analyzed how human papillomavirus (hpv) vaccination was portrayed in videoclips and viewer-posted comments available on youtube.200818675530
a critique of criteria for evaluating vaccines for inclusion in mandatory school immunization programs.several new vaccines for children and young adults have been introduced recently and now appear on the advisory committee on immunization practices' recommended childhood and adolescent immunization schedule (meningococcal, rotavirus, human papillomavirus). as new vaccines are introduced, states face complex decisions regarding which vaccines to fund and which vaccines to require for school or child care entry. this complexity is evidenced by the current debate surrounding the human papillomavir ...200818676536
new approaches to external genital warts and vulvar intraepithelial neoplasia.human papillomavirus is the most common sexually transmitted disease in the united states. for the majority of affected individuals, the virus remains subclinical. however, human papillomavirus infection may result in a broad spectrum of vulvar disease including genital warts, dysplasia, and invasive carcinoma. we review the evaluation and currently available therapies to assist in patient management.200818677144
issues in human papillomavirus vaccination in adolescents. 200818809142
age-specific prevalence of infection with human papillomavirus in females: a global review.global data on age-specific prevalence of human papillomavirus (hpv) infection overall, and for high-risk hpv types 16 and 18, are essential for the future implementation of hpv prophylactic vaccines for cervical cancer prevention.200818809145
potential barriers to hpv vaccine provision among medical practices in an area with high rates of cervical cancer.potential barriers to widespread vaccination of adolescent girls against human papillomavirus (hpv) infection are poorly understood. we provide an overview of potential barriers to provision of hpv vaccine and empirical data on the concerns of medical practices that may inhibit hpv vaccine provision.200818809147
trade-offs in cervical cancer prevention: balancing benefits and risks.new screening and vaccination technologies will provide women with more options for cervical cancer prevention. because the risk of cervical cancer diminishes with effective routine screening, women may wish to consider additional attributes, such as the likelihood of false-positive results and diagnostic procedures for mild abnormalities likely to resolve without intervention in their screening choices.200818809815
oral human papillomavirus lesion identified using velscope instrumentation: case report.the role of oral human papillomavirus (hpv) in the incidence of oral carcinoma has increased among traditional non-risk patients under the age 40. this case study describes how a squamous papilloma (attributed to the hpv) was discovered using the velscope oral screening device. photographs of the lesion that might help dentists to identify these areas, using both white light and fluorescence visualization with velscope, are included.200818810914
hiv-1 tat enhances replicative potential of human oral keratinocytes harboring hpv-16 genome.introducing highly active antiretroviral therapy (haart) has significantly decreased the morbidity and mortality in human immunodeficiency virus-positive (hiv+) individuals by decreasing the viral loads and increasing the cd4+ t-cell counts. subsequently, the occurrence of many hiv-associated diseases has been dramatically declined except human papillomavirus (hpv)-associated lesions. such notion suggests that immune response is not a major determinant, and that the direct interaction between hi ...200818813791
infection: is it a cause of bladder cancer?this article reviews the literature regarding the possible correlation between infection and occurrence of bladder cancer. the pubmed literature database was searched from inception to january 2008. keywords of bladder, cancer, parasitic, bacterial, viral and infection, were used. forty studies were included in the review. several investigators support the idea that schistosomiasis is aetiologically related to the development of bladder cancer in individuals infected with schistosoma haematobium ...200818815920
cell-restricted immortalization by human papillomavirus correlates with telomerase activation and engagement of the htert promoter by myc.the high-risk human papillomaviruses (hpvs) are the causative agents of nearly all cervical cancers and are etiologically linked to additional human cancers, including those of anal, oral, and laryngeal origin. the main transforming genes of the high-risk hpvs are e6 and e7. e6, in addition to its role in p53 degradation, induces htert mrna transcription in genital keratinocytes via interactions with myc protein, thereby increasing cellular telomerase activity. while the hpv type 16 e6 and e7 ge ...200818818322
association of hsp90 to the htert promoter is necessary for htert expression in human oral cancer cells.enhanced expression of human telomerase reverse transcriptase (htert) occurs frequently during cellular immortalization. the current study was undertaken to determine the mechanism regulating the htert promoter activity during cellular immortalization of human oral keratinocytes. normal human oral keratinocytes (nhoks) were immortalized with bmi-1 and the e6 oncoprotein of human papillomavirus type 16 to establish the telomerase-positive hok-bmi-1/e6 cell line. using dna-protein-binding assay, w ...200818820283
men's attitudes toward receiving the human papillomavirus vaccine.to determine men's attitudes toward receiving the human papillomavirus (hpv) vaccine.200818820541
the epidemiology and burden of hpv disease.margaret stanley describes what human papillomavirus is and how it causes disease. she also outlines how the new vaccine to protect against hpv works.200818822547
characterization of humoral immune responses against p16, p53, hpv16 e6 and hpv16 e7 in patients with hpv-associated cancers.the cellular tumor suppressor p16 is strongly overexpressed in cervical cancers and precancers. we have previously demonstrated that infiltrating t lymphocytes reactive against p16 can be found in cervical cancer patients. here, we analyzed whether p16 induces humoral immune responses. sera of patients with cervical cancer, oropharyngeal cancer, colorectal cancer and autoimmune disease were included. a total of 919 sera were analyzed, including 486 matched sera from a cervical cancer case contro ...200818785210
molecular concepts of virus infections causing skin cancer in organ transplant recipients.globally approximately 15% of all malignant tumors are caused by viruses and even a higher percentage is observed in organ transplant recipients (otr). here, nonmelanoma skin cancer (nmsc) is the most frequent malignancy, which correlates with cutaneous human papilloma virus (hpv) infection. in the present review, we reflect on some recent general concepts how tumor viruses can either act as direct or indirect carcinogens in the multistep process of carcinogenesis. immunosuppressive drugs in otr ...200818785959
human papillomavirus type 16 (hpv-16) viral load and persistence of hpv-16 infection in women infected or at risk for hiv.persistent hpv-16 infection is a marker for risk of progression to high-grade cervical lesions. the predictive value of hpv-16 viral loads for persistent hpv-16 infection was assessed longitudinally in a cohort of 1055 sexually active women.200818786854
production of biologically active cxc chemokines by lactococcus lactis: evaluation of its potential as a novel mucosal vaccine adjuvant.chemokines have been described as essential mediators in leukocytes migration to inflammatory sites and to secondary lymphoid organs. mig and ip-10 are two cxc chemokines that recruit mononuclear cells in vivo and inhibit angiogenesis. in addition to their chemotactic roles, mig and ip-10 have also an important role in the adaptative immune response. in this study, we asked whether a food-grade bacterium, lactococcus lactis, is able to produce a fusion protein comprising mig and ip-10 (mig::ip-1 ...200818790708
[prevalence and cervical human papilloma virus associated factors in patients with rheumatoid arthritis].nevertheless its association with cervicouterine cancer, there is no information about cervical human papillomavirus infection prevalence in patients with rheumatoid arthritis.200818798391
persistence and clearance of human papillomavirus infection: a prospective cohort study.the objective of the study was to identify epidemiological correlates for persistence and clearance of human papillomavirus (hpv) infection.200818799155
analysis of human papillomavirus type 16 (hpv16) dna load and physical state for identification of hpv16-infected women with high-grade lesions or cervical carcinoma.integration of human papillomavirus (hpv) dna into the host cell genome is a frequent event in cervical carcinogenesis, even though this phenomenon does not seem to be mandatory for cervical cancer development. our objective was to describe the load and physical state of hpv type 16 (hpv16) dna in a series of cervical samples representative of the natural history of cervical cancer. we used a combination of three real-time pcr assays targeting e6, e2, and albumin genes to calculate hpv16 load (e ...200818799702
top tips in 2 minutes. 200818801299
comment on "local accumulation of foxp3+ regulatory t cells: evidence for an immune evasion mechanism in patients with large condylomata acuminata". 200818802042
cervical cancer vaccination: the real meaning of cross-protection and its impact on the prevention.prophylactic vlp-based vaccines effectively prevent papillomavirus infections with a high level of antibodies and safety. an 18% increased efficacy against cin 2+ lesions associated with hpvs 16/18 has been observed with the appliance of the hpv16/18 as04 adjuvanted recombinant vaccine.200818804508
a new hpv-dna test for cervical-cancer screening in developing regions: a cross-sectional study of clinical accuracy in rural china.a new test (carehpv; qiagen, gaithersburg, md, usa) has been developed to detect 14 high-risk types of carcinogenic human papillomavirus (hpv) in about 2.5 h, to screen women in developing regions for cervical intraepithelial neoplasia (cin). we did a cross-sectional study to assess the clinical accuracy of carehpv as a rapid screening test in two county hospitals in rural china.200818805733
comparison of patient- and clinician-collected anal cytology samples to screen for human papillomavirus-associated anal intraepithelial neoplasia in men who have sex with men.human papillomavirus (hpv)-associated anal cancer is increasing in prevalence and is more common among men who have sex with men and hiv-positive individuals than cervical cancer is among women in the united states. cytology screening can detect the anal cancer precursor, anal intraepithelial neoplasia (ain). little is known about self-collected samples for ain screening, and few community-based ain estimates exist.200818765699
decreased survival in egfr gene amplified vulvar carcinoma.we undertook an extensive molecular characterization of the epidermal growth factor receptor (egfr) gene in vulvar squamous cell carcinomas to investigate egfr mutation and/or genomic amplification and its association with egfr protein expression, high-risk human papillomavirus (hpv) status and clinical outcome.200818768215
p16 ink4a and hpv l1 immunohistochemistry is helpful for estimating the behavior of low-grade dysplastic lesions of the cervix uteri.as only a minority of low-grade dysplastic lesions of the cervix uteri will eventually progress to carcinoma, predicting the behavior of these lesions could be of high value in clinical practice. the aim of the study was to evaluate p16 ink4a and l1 as immunohistochemical markers of the biologic potentiality of low-grade dysplasia of the uterine cervix. the study included 38 conization specimens with coexisting cervical intraepithelial neoplasia grade 1 (cin1) and 3 (cin3) (group a) and 28 punch ...200818769337
establishment and characterization of immortalized human gingival keratinocyte cell lines.primary human keratinocytes are used to analyze the properties of the oral epithelium and the early stages of oral bacterial infections. in vitro, these cells are characterized by their short life span and restricted availability. approaches for culturing these cells will end after approximately 6-10 passages as a result of entry into apoptosis. for this reason, it is important to generate cell lines suitable for obtaining an unlimited source of cells. therefore, the aim of the present study was ...200818771458
inhibition of human papillomavirus replication by using artificial zinc-finger nucleases.recently, we have designed artificial zinc-finger proteins (azps) that prevent a viral replication protein, e2, of human papillomavirus type 18 (hpv-18) from binding to its replication origin and demonstrated that the gene-delivered azps inhibited hpv-18 dna replication in mammalian cells. in the present study, we examined a new approach to inhibition of dna virus replication by using an azp-nuclease fusion. in transient replication assays for hpv-18, the gene-delivered azp-nuclease fusion reduc ...200818776315
in gfp with high risk hpv-18e6 fusion protein expressed 293t and mcf-7 cells, the endogenous wild-type p53 could be transiently phosphorylated at multiple sites.infected cells recognize viral replication as a dna damage stress and elicit the host surveillance mechanism to anti-virus infection. modulation of the activity of tumor suppressor p53 is a key event in the replication of many viruses. they could manipulate p53 function through phosphorylation modification for their own purpose. but there is rarely research about p53 phosphorylation status in the context of hpv-e6. therefore, we investigated whether p53 could be phosphorylated by hpv-e6.200818778462
[transcriptional repressive activity of mutated e2 protein of human papillomavirus 2 (hpv-2) variant].common warts are close associated with hpvs infection. in this study, we amplified and sequenced the lcr fragment and e2 gene of hpv-2 that infected the patient of extensive common wart with cutaneous horns, and we constructed the recombinant cat-reporter plasmids pblcat-lcr containing hpv-2 prototype or variant lcr and mammalian expression plasmids pcdna3. 1-e2 containing prototype or variant e2 orf individually. the promoter activities of hpv-2 variant and the transcriptional repression activi ...200818780628
recent advances in head and neck cancer. 200818784104
validation of an automated detection platform for use with the roche linear array human papillomavirus genotyping test.an automated platform (beeblot) was evaluated in parallel with the recommended protocol for the hybridization and detection steps of the roche linear array human papillomavirus (hpv) genotyping test using dna from 143 cervical specimens. genotyping profiles showed 100% concordance between the methods, suggesting that automation could complement the roche linear array for enhanced speed and reproducibility.200818784316
the e8--e2 gene product of human papillomavirus type 16 represses early transcription and replication but is dispensable for viral plasmid persistence in keratinocytes.a conserved e8(wedge)e2 spliced mrna is detected in keratinocytes transfected with human papillomavirus type 16 (hpv-16) plasmid dna. expression of hpv-16 e8--e2 (16-e8--e2) is independent of the major early promoter, p97, and is modulated by both specific splicing events and conserved cis elements in the upstream regulatory region in a manner that differs from transcriptional regulation of other early viral genes. mutations that disrupt the predicted 16-e8--e2 message also increase initial hpv- ...200818753207
functional mapping of the human papillomavirus type 16 e1 cistron.replication of the double-stranded, circular human papillomavirus (hpv) genomes requires the viral dna replicase e1. here, we report an initial characterization of the e1 cistron of hpv type 16 (hpv-16), the most common oncogenic mucosal hpv type found in cervical and some head and neck cancers. the first step in hpv dna replication is an initial burst of plasmid viral dna amplification. complementation assays between hpv-16 genomes carrying mutations in the early genes confirmed that the expres ...200818753208
squamous intraepithelial lesions arising in benign endocervical polyps: a report of 9 cases with correlation to the pap smears, hpv analysis, and immunoprofile.endocervical polyps are common benign lesions that typically arise in the endocervical canal of women from 40 to 60 years old. the reported incidence of squamous intraepithelial lesion (sil) occurring in an endocervical polyp is typically less than 1/1,000 (0.1%). we report on 9 cases of sil arising in otherwise classic benign endocervical polyps; these lesions were from a pool of about 1500 endocervical polyps for an incidence of approximately 0.5%. five cases were low-grade sil; of the 4 cases ...200818753960
hpv detection and genotyping as an earlier approach in cervical cancer screening of the female genital tract.human papillomavirus (hpv) infection is the leading risk factor for cervical intraepithelial neoplasia (cin) and cervical cancer. more than 100 virus genotypes have been identified so far, some of them strongly associated with the development of neoplasia. the aim of this study was to evaluate the prevalence of the different hpv genotypes in women presenting no cytological alterations in cervical cells, in women presenting light alterations, and in women presenting severe alterations at routine ...200818754286
high-risk human papilloma viruses (hpvs) were not detected in the benign skin lesions of a small number of children.human papilloma virus (hpv) can be transmitted via sexual as well as nonsexual routes. recently, 'high-risk' hpvs were detected in the oral mucosa of children in whose cases there was no suspicion of sexual abuse. this implies that hpv 16 and 18 have additional nonsexual modes of transmission in childhood, such as vertical transmission and autoinoculation.200818754827
[role of p16ink4a as a biomarker in liquid-based cervical cytology screening].in cervical lesions, the overexpression of p16ink4a has been reported to be closely associated with human papillomavirus (hpv) infection. this study is designed to evaluate the role of p16ink4a as a biomarker in liquid-based cervical cytology screening.200818756939
self-disclosure of sexually transmitted diseases: an integrative review.in the united states, there are currently more than 65 million individuals living with a sexually transmitted disease (std), and approximately 19 million new infections will continue to occur each year. to decrease the transmission of potentially dangerous and life-altering stds, self-disclosure of sexual and drug history and sexual orientation is paramount. this integrative review examines the concept of self-disclosure of stds, suggests areas of additional research, and proposes a comprehensiv ...200818758276
p53 codon 72 polymorphism and hpv status in lung cancer.several risk factors, both environmental and genetic, have been associated with the pathogenesis of lung cancer. a common polymorphism at codon 72 of exon 4 of the p53 gene encoding either an arginine or proline has been shown to confer susceptibility to the development of different human malignancies. this polymorphism affects proteolytic degradation of p53 promoted by the hpv-e6 protein and represents a risk factor for human-papillomavirus-induced carcinogenesis.200818758421
prognostic factors and survival analysis in a sample of oral squamous cell carcinoma patients.the aims of this report were to describe the 5-year overall survival (os) in a group of oral squamous cell carcinoma (oscc) patients and to investigate the effects of age, gender, anatomic localization, tumor evolution time, smoking and alcohol intake, nodal status, tumoral recurrences, histologic classification, p53 and p63 immunoexpression, human papillomavirus dna presence, and treatment on the prognostic outcome.200818760943
human papillomavirus vaccine risk and reality. 200818762616
the human papillomavirus vaccine and risk of anaphylaxis. 200818762617
awareness of human papillomavirus in a cohort of nearly 70,000 women from four nordic countries.to assess the fraction of women who have heard of human papillomavirus (hpv) in four nordic countries (denmark, iceland, norway, and sweden) and to study the correlates of this awareness, just before the vaccines were released.200818763170
emerging antiviral drugs.foremost among the newly described antiviral agents that may be developed into drugs are, for the treatment of human papilloma virus (hpv) infections, cprpmedap; for the treatment of herpes simplex virus (hsv) infections, bay 57-1293; for the treatment of varicella-zoster virus (vzv) infections, fv-100 (prodrug of cf 1743); for the treatment of cytomegalovirus (cmv) infections, maribavir; for the treatment of poxvirus infections, st-246; for the treatment of hepatitis b virus (hbv) infections, t ...200818764719
bap31 is a novel target of the human papillomavirus e5 protein.the e5 proteins of human papillomaviruses (hpvs) are small hydrophobic proteins that are expressed in the early and late stages of the viral life cycle; however, their role in hpv pathogenesis is not clearly understood. in this study, a split-ubiquitin yeast (saccharomyces cerevisiae) two-hybrid system was used to identify b-cell-associated protein 31 (bap31) as a binding partner of hpv e5 proteins. the association of these proteins was confirmed by coimmunoprecipitation of complexes of bap31 wi ...200818684816
specific up-regulation of dna polymerase by human papillomavirus 16.to analyze how the infection of human papillomavirus 16 (hpv16) affects expression of dna polymerase beta (dna polb) with the aim of probing the mechanism of over-expression of dna polb in human cancers.200818686630
[vaccination against the human papilloma virus. social and health implications in spain].secondary prevention of cervical cancer is carried out by means of screening programs. the possibility of primary prevention by means of a vaccination has been a reality since mid-2006. two vaccinations exist; one tetravalent and the other bivalent. both have proven to be capable to prevent cervical cancer precursory lesions with few secondary effects. the proscribed age to administer this vaccination for all girls, at 12 years of age, implies some treatment problems. over the long term, there i ...200818689209
isolation and cloning of human papillomavirus 16 l1 gene from iranian isolate.to isolate and construct cloning and expression vectors containing human papillomavirus (hpv16-l1) gene as target for application as recombinant vaccine.200818690300
an optimized yeast cell-free system: sufficient for translation of human papillomavirus 58 l1 mrna and assembly of virus-like particles.we developed a yeast cell-free system suitable for in vitro translation of human papillomavirus 58 (hpv58) l1 mrna. this system was systematically optimized resulting in enhanced translation efficiency. the optimal concentrations of potassium and magnesium ions observed were specific to the hpv58 l1 protein production. supplementation with sucrose in the preparation of the yeast lysate greatly enhanced its stability. after optimization, protein production in this system was significantly superio ...200818691524
seroepidemiology as basis for design of a human papillomavirus vaccination program.we have performed a serological survey of hpv type 16-antibody prevalence by age and sex in sweden and used it as a basis for modelling the optimal vaccination strategies in this population. samples of 3,317 subjects were tested for hpv16-specific antibodies. the observed age-specific seroprevalences along with sexual behaviour data were used to infer parameter values for a mathematical model representing sweden and the preventive effect of possible strategies estimated. by the year 2055, vaccin ...200818692109
the human papillomavirus type 8 e2 gene encodes a transforming activity sufficient for skin tumor formation in transgenic mice.infections with beta-genus human papillomaviruses (hpvs) have been associated with nonmelanoma skin cancers, particularly in immunocompromised patients and individuals with a rare genetic disease, epidermodysplasia verruciformis (ev). using a transgenic mouse model, herbert pfister's group determined that expression of the hpv8 e2 gene results in skin cancer development and that this process is greatly accelerated by uv irradiation (pfefferle et al., 2008, this issue).200818695686
possible role for cellular karyopherins in regulating polyomavirus and papillomavirus capsid assembly.polyomavirus and papillomavirus (papovavirus) capsids are composed of 72 capsomeres of their major capsid proteins, vp1 and l1, respectively. after translation in the cytoplasm, l1 and vp1 pentamerize into capsomeres and are then imported into the nucleus using the cellular alpha and beta karyopherins. virion assembly only occurs in the nucleus, and cellular mechanisms exist to prevent premature capsid assembly in the cytosol. we have identified the karyopherin family of nuclear import factors a ...200818701594
effect of community consultation on recruitment of indigenous women to a human papillomavirus prevalence study. 200818707573
does pretreatment seropositivity to human papillomavirus have prognostic significance for head and neck cancers?human papillomavirus (hpv) is a risk factor for head and neck cancers (hnc), yet hpv-associated tumors have better prognosis than hpv-negative tumors.200818708401
the analysis of the entire hla, partial non-hla and hpv for chinese women with cervical cancer.human leukocyte antigen (hla) alleles have been associated with an increased or decreased risk of developing cervical cancer through infection with oncogenic forms of the human papillomavirus (hpv). the aim of this study was to analyze the interactions between hpv and hla, particularly of the entire hla and partial non-hla regions, in invasive cervical cancers. the results showed the distribution of hpv genotypes among patients with cervical cancer in this group: the overall prevalence of hpv dn ...200818712835
ethnicity determines association of p53arg72pro alleles with cervical cancer in china.minority uigur women residing in xinjiang, in the northwest of china, have a high incidence of cervical carcinoma (cc; 527/100 000) and are often diagnosed young. we favor the hypothesis that uigur women may carry different genetic factor(s) making them more susceptible to cc than majority han (chinese) women living in the same region. using pcr-restriction fragment length polymorphism, we investigated associations of a p53arg72pro polymorphism with cc in uigur women compared with those in han w ...200818714189
cost of screening and treatment of cervical dyskaryosis in germany.human papillomavirus (hpv) infection is the principal cause of cervical cancer. clinical trials with hpv vaccines have shown high efficacy against hpv-induced precancerous cervical lesions. before implementing a vaccination programme, up-to-date data on cervical dyskaryosis, incidence and annual treatment costs are needed. we assessed resource use and costs for 12 months following diagnosis for women with abnormal pap smears in germany based on a sample of 138 women who had received abnormal res ...200818714567
perianal squamous cell carcinoma in-situ: a report of two human papilloma virus-negative cases.abstract: we are reporting two cases of perianal squamous cell carcinoma in-situ, negative for high-risk (hr) and low-risk (lr) human papilloma viruses. a brief review of anal and perianal squamous cell carcinoma and the role of hpv are presented.200818715505
Refresh
firstprev
...11121314151617181920...
nextlast
Displaying items 1501 - 1600 of 6259