Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| cloning, sequencing, and transcriptional regulation of the vibrio cholerae fur gene. | many genes involved in the transport of iron by bacteria as well as in pathogenesis are regulated by the environmental concentration of iron, with increased expression under low-iron conditions. in escherichia coli, transcriptional regulation by iron depends on the fur gene. a virulence gene in vibrio cholerae (irga) is also transcriptionally regulated by iron, and the promoter of irga contains a dyad repeat homologous to fur binding sites in e. coli. southern hybridization of v. cholerae chromo ... | 1992 | 1372314 |
| effects of neuraminidase on airway reactivity in the guinea pig. | we investigated the effects of neuraminidase, a viral enzyme that cleaves alpha ketosidic cell-bound sialic acids, to see if it accounts for parainfluenza and influenza virus-induced airway hyperreactivity. accordingly, vibrio cholerae neuraminidase was administered intratracheally in guinea pigs, and airway reactivity was assessed 3 h later. removal of sialic acid residues was evaluated by histologic studies. airway responsiveness was determined in anesthetized, tracheotomized, and mechanically ... | 1992 | 1372796 |
| serotype conversion in vibrio cholerae o1. | vibrio cholerae o1 exists as two major serotypes, inaba and ogawa, which are associated with the o antigen of the lipopolysaccharide and are capable of unequal reciprocal interconversion. the 20-kilobase rfb regions encoding o-antigen biosynthesis in strains 569b (inaba) and o17 (ogawa) have been cloned in escherichia coli k-12 and the nucleotide sequences have been determined. besides several base substitutions and a small deletion in the 569b sequence relative to o17, there is a single nucleot ... | 1992 | 1372980 |
| influenza viruses differ in recognition of 4-o-acetyl substitution of sialic acid receptor determinant. | equine alpha 2-macroglobulin (em), known to contain both neu5ac and neu4,5ac2 sialic acid residues, was treated with vibrio cholerae sialidase for the selective removal of neu5ac and was compared with the untreated em for its binding by a panel of influenza viruses. type a h3n2 virus strains having leu in position 226 of their hemagglutinin (ha) changed the affinity for sialidase-treated em only slightly, if at all, indicative of their ability to bind the 4-o-ac-substituted neu5ac receptor deter ... | 1992 | 1374985 |
| differential sensitivity of cd34 epitopes to cleavage by pasteurella haemolytica glycoprotease: implications for purification of cd34-positive progenitor cells. | our previous studies have shown that a unique glycoprotease from pasteurella haemolytica specifically cleaves only proteins containing sialylated o-linked glycans. the hematopoietic progenitor cell antigen, cd34, which is heavily glycosylated with both n- and o-linked glycans, is readily cleaved by this protease. in this study, we demonstrate that the epitopes detected by five of the seven cd34 monoclonal antibodies are removed by the glycoprotease. the differential sensitivity of the cd34 epito ... | 1992 | 1375160 |
| inhibition of adhesion of s-fimbriated escherichia coli to buccal epithelial cells by human milk fat globule membrane components: a novel aspect of the protective function of mucins in the nonimmunoglobulin fraction. | we investigated the presence of factors in human milk that inhibit invasion of pathogenic bacteria. the effect of human milk fat globule membrane (hmfgm) components on adhesion of cloned s-fimbriated escherichia coli to human buccal epithelial cells was analyzed. s fimbriae are a common feature of e. coli strains causing sepsis and meningitis in newborns and are bound to epithelia via sialyl-(alpha-2-3)galactoside structures. human milk fat globules (hmfg) could be agglutinated by the above-ment ... | 1992 | 1377184 |
| [development of oral vaccines based on recombinant proteins derived from cholera toxin]. | in this paper a new approach to create antigens through genetic engineering is discussed. in this particular case the subunits of v. cholerae toxin are used as heterologous epitope carries. in this paper the manipulation of a and b subunits is described. this manipulation allows both the insertion of epitopes to the b subunit and the use of subunit a in the construction of recombinant antigens similar to the ones derived from subunit b. | 1992 | 1377408 |
| histochemical analysis of sialomucin in paget cells of mammary and extramammary paget's disease. | the cytoplasmic sialomucin in paget cells of both mammary and extramammary paget's diseases was examined, using a new method proposed by volz et al. (1987a, b). the staining methods used involved an electrolyte-alcian blue (ph = 5.8) and periodic acid schiff. oxidation was performed with 0.4 mmol/l periodate in 1 mol/l hcl at 4 degrees c or 50 mmol/l periodate in distilled water at room temperature for 1 h. methylation, saponification, borohydride reduction, and digestion with diastase, neuramin ... | 1992 | 1379408 |
| 'in vitro' action of histamine and cimetidine on amylase secretion in the rat pancreas. | we report here the effect of histamine on amylase secretion in isolated lobules from the rat pancreas. different concentrations of histamine increased amylase secretion, while the stimulatory effect was reduced by cimetidine although not by chlorpheniramine. these findings suggest that pancreatic lobules have h2 receptors. on the other hand, bethanechol induced a stimulatory effect on pancreatic lobules that was inhibited by cimetidine. vibrio cholerae toxin stimulated amylase secretion, but thi ... | 1992 | 1379840 |
| the vascular endothelial growth factor proteins: identification of biologically relevant regions by neutralizing monoclonal antibodies. | angiogenesis plays critical roles in organ development during embryonic and fetal life, wound healing and in a variety of pathological conditions. vascular endothelial growth factor (vegf) is a secreted growth factor specific for vascular endothelial cells which induces angiogenesis in vivo. to gain a better understanding of the physiological role of vegf, we have generated and characterized four murine monoclonal antibodies (mabs) using the 165 amino acid species of recombinant human vegf as im ... | 1992 | 1380254 |
| the adjuvant effect of vibrio cholerae and escherichia coli heat-labile enterotoxins is linked to their adp-ribosyltransferase activity. | this study addressed the question of whether the mucosal adjuvant property of cholera toxin (ct) and the structurally closely related escherichia coli heat-labile toxin (lt) requires the enterotoxic and adenylate cyclase/camp activating property of these molecules. therefore, we investigated the cytotoxic and adjuvant abilities of the enterotoxins and compared the results with those obtained with the non-toxic ct and lt derivatives; recombinant ctb (rctb) and a mutated lt (mlt), which had a sing ... | 1992 | 1381311 |
| serological cross-reaction between yersinia enterocolitica o9 and non-o1 vibrio cholerae bio-serogroup hakata and antigenic analysis of their relationship by their lipopolysaccharides. | cross-agglutination and cross-agglutinin absorption experiments were carried out on non-o1 vibrio cholerae bio-serogroup hakata (hakata) and yersinia enterocolitica o9 (o9). it was shown that the o-antigen of hakata was closely related to that of o9 in an a, b-a, c type of relationship. the antigenic relationship between the o-antigens of the two bacteria was analyzed by passive hemolysis (ph) and passive hemolysis inhibition (phi) tests by using their lipopolysaccharides (lps) as antigen for se ... | 1992 | 1381806 |
| disparate effects of calcium channel blockers on pressure dependence of renin secretion and flow in the isolated perfused rat kidney. | using the model of isolated perfused rat kidneys this study was performed to investigate whether or not voltage-operated calcium channels are essentially involved in the pressure control of renin secretion from the kidneys. at a perfusion pressure of 100 mm hg (13.3 kpa) renin secretory rates were 4.2 +/- 0.7 (ng angiotensin i h-1) min-1 g-1. stepwise reduction of renal perfusion pressure to 80, 60, and 40 mm hg (10.6, 8.0, 5.3 kpa) resulted in an increase of renin release yielding a 30-fold sti ... | 1992 | 1382264 |
| cholera in 1991. | 1992 | 1382534 | |
| the mode of action of vibrio cholerae cytolysin. the influences on both erythrocytes and planar lipid bilayers. | the interaction with erythrocytes of cholera cytolysin (cc) obtained from a non-01 vibrio cholerae strain results in the osmotic rupture of target cells upon formation by cc of the waterfilled pores in their membranes. the aggregation of several toxin monomers is required for the formation of one cc channel with a radius of 0.9-1.0 nm. the investigations using planar bilayer lipid membranes suggest that the cc-induced pore is an interprotein anion selective channel carrying a fixed positive char ... | 1992 | 1382601 |
| construction of the physical maps of mycoplasma hyopneumoniae and mycoplasma flocculare and the location of rrna genes on these maps. | the genomes of mycoplasma flocculare and mycoplasma hyopneumoniae, two mycoplasmas of the porcine respiratory system, were studied. based upon antigenic cross-reactivity and dna-dna hybridization, these species have given indication of a close genetic relationship. by using field-inversion gel electrophoresis and employing the restriction digest fragments obtained from the gels as the probes, physical maps of the genomes of the two species were constructed. mycoplasma hyopneumoniae is similar to ... | 1992 | 1382823 |
| the treatment of simultaneous choriocarcinoma in mother and baby. | simultaneous occurrence of choriocarcinoma in mother and child is rare. such a case is described in which a massive intrahepatic tumor in the infant led to its treatment and that of the mother who showed evidence of the same tumor. both are alive and well 1 year after treatment, the longest recorded infant survival. | 1992 | 1383486 |
| [study of the epidemic significance of noncultivated forms of vibrio cholerae by the polymerase chain reaction]. | a specific method of the isolation of the cholera toxin gene by the directional amplification of dna in the polymerase chain reaction (pcr) has been developed. the product of this reaction has a molecular weight of 440 sequence pairs and is a dna fragment located on the a-subunit of v. cholerae gene vct. the sensitivity of the method permits the detection of one bacterial cell in the reaction mixture. the method is effective when v. cholerae purified dna, cell lysates and the dna of total microf ... | 1992 | 1387276 |
| [antibiotic sensitivity of vibrio cholerae 01 isolated from environmental objects]. | the sensitivity of 252 vibrio cholerae-o1 strains isolated from environmental objects to antibiotics of various groups was assayed by the method of serial dilutions on solid media. the biological characteristics of the isolates are presented. the vibrio cholerae isolates with serological variation were the most frequent (36.6 per cent), so are the cultures detected by their sensitivity to the specific phages (87.5 per cent). it was found that changes in some biological properties of the strains ... | 1992 | 1387519 |
| influence of dopaminergic and serotonergic neurons on intravenous ethanol self-administration in the rat. | rats implanted with chronic indwelling intravenous catheters and allowed access to a self-administration apparatus learned to self-inject intravenous ethanol. ethanol concentrations of 0.5, 1.0, and 2.0%, corresponding to a dose/injection of 1, 2, and 4 mg/kg, respectively, were consistently self-injected. self-injection was not acquired or maintained with ethanol doses of 0.5 or 8 mg/kg/injection. saline replacement of ethanol reservoirs led to marked increases in lever-pressing response in ani ... | 1992 | 1388276 |
| suppression of the arthus reaction by y-24180, a potent and specific antagonist of platelet-activating factor. | a novel and potent antagonist of platelet-activating factor (paf), y-24180 (4-(2-chlorophenyl)-2-[2-(4-isobutylphenyl)ethyl]-6,9-dimethyl-6h- thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4] diazepine) was investigated for the effects on the skin reactions induced by chemical mediators and the arthus reactions. in the rat dorsal skin, y-24180 (0.1-10 mg/kg, p.o.) inhibited increase in vascular permeability by the intradermal paf injection in a dose dependent manner and the inhibitory activity was 60 tim ... | 1992 | 1388318 |
| cholera as a model for research on mucosal immunity and development of oral vaccines. | during the past year, the extensive investigational use of a recently developed oral vaccine against cholera has led to significant advances in our understanding of both immunity to cholera and related diarrhoeal diseases, and the mucosal immune response in general after oral immunization. the oral cholera vaccine has been shown to protect, through its cholera toxin b subunit component, against travellers' diarrhoea caused by enterotoxigenic escherichia coli. the elaboration of sensitive new tec ... | 1992 | 1388838 |
| [diarrhea in a woman from peru]. | 1992 | 1391004 | |
| cholera. | 1992 | 1392799 | |
| cytochemical characterization of the cuticle of caenorhabditis elegans (nematoda: rhabditoidea). | at the ultrastructural level, the caenorhabditis elegans (maupas, 1900; doughert, 1953) cuticle shows the presence of six layers: epicuticle, external cortical, internal cortical, intermediate, fibrous and basal. two techniques were used for carbohydrate localization: the periodic acid-thiosemicarbazide-silver proteinate (thiéry) and gold-labelled lectins. no labelling was found on the nematode's cuticle. with the ethanolic phosphotungstic acid technique (e-pta), that detects basic proteins, rea ... | 1992 | 1394094 |
| foot infections in diabetes are rarely due to a single microorganism. | the purpose of this study was to investigate the frequency with which multiple organisms can be isolated from severe foot infections in diabetic patients with carefully planned techniques of collection and processing of specimens. one hundred and seventy-seven organisms were isolated from 52 patient admissions (mean 3.4 per infection) at a district hospital. this was compared to a mean of 2.1 organisms per lesion at a local university hospital and 2.3 at a smaller community hospital. staphylococ ... | 1992 | 1395469 |
| cyclosporin a treatment of young children with newly-diagnosed type 1 (insulin-dependent) diabetes mellitus. london diabetes study group. | several studies have demonstrated the efficacy of cyclosporin a in modifying the initial course of type 1 (insulin-dependent) diabetes mellitus in older children and adults but none have reported the effects in very young children. we treated 14 newly-diagnosed type 1 diabetic patients aged 22 months to 95 months with cyclosporin a. mean insulin dose at entry was 0.7 +/- 0.07 iu.kg-1.day-1. initial cyclosporin a dose was 10 mg.kg-1.day-1. insulin dose reached a nadir of 0.13 iu.kg-1.day-1 by 180 ... | 1992 | 1397785 |
| genetics of cholera toxin. | cholera is caused by the toxin secreted by vibrio cholerae 01. cholera toxin (ct) is a protein consisting of a and b subunits. the former contributes to intracellular toxicity whereas the b subunit is required for binding of ct to eukaryotic cell surface receptor. the structural genes encoding a and b subunits are designated as ctxa and ctxb respectively. these genes are located on the chromosome forming an operon in which ctxa precedes ctxb. the ctxab have been cloned and sequenced. classical s ... | 1992 | 1398801 |
| association of giardia lamblia with vibrio in cholera cases. | 1992 | 1398879 | |
| major outer membrane proteins of vibrio cholerae and their role in induction of protective immunity through inhibition of intestinal colonization. | vibrio cholerae o1 organisms belonging to different biotypes and serotypes were shown to express major outer membrane proteins (momps) with subunit molecular masses of 48 to 50, 40 to 43, 35 to 36, 27 to 28, and 20 kda. antisera raised against individual momps of a v. cholerae o1 strain recognized momps of corresponding molecular masses in other o1 and non-o1 strains. serological data also suggested possible differences in the cell surface exposition of these momps. however, no marked difference ... | 1992 | 1398996 |
| fusion proteins containing the a2 domain of cholera toxin assemble with b polypeptides of cholera toxin to form immunoreactive and functional holotoxin-like chimeras. | cholera enterotoxin (ct) is produced by vibrio cholerae and excreted into the culture medium as an extracellular protein. ct consists of one a polypeptide and five b polypeptides associated by noncovalent bonds, and ct-b interacts with ct-a primarily via the a2 domain. treatment of ct with trypsin cleaves ct-a into a1 and a2 fragments that are linked by a disulfide bond. ct-b binds to ganglioside gm1, which functions as the plasma membrane receptor for ct, and the enzymatic activity of a1 causes ... | 1992 | 1399002 |
| a 14-kilodalton inner membrane protein of vibrio cholerae biotype e1 tor confers resistance to group iv choleraphage infection to classical vibrios. | choleraphage phi 149 differentiates the two biotypes, classical and el tor, of vibrio cholerae. this phage cannot replicate in v. cholerae biotype el tor cells because the concatemeric dna intermediates produced are unstable and cannot be chased to mature phage dna. a v. cholerae biotype el tor gene coding for a 14,000-da inner membrane protein which destabilizes the concatemeric dna intermediates by hindering their binding to the cell membrane has been identified. presumably, a 22,000-da v. cho ... | 1992 | 1400172 |
| toxr proteins with substitutions in residues conserved with ompr fail to activate transcription from the cholera toxin promoter. | the toxr protein of vibrio cholerae is an integral membrane protein that coordinately regulates the expression of virulence genes required for successful infection. toxr has been shown to bind directly to and activate transcription of the cholera toxin (ctx) promoter. within the amino-terminal cytoplasmic region of toxr, several amino acids are strictly conserved among toxr, ompr, and the other members of a family of bacterial regulatory proteins. to better understand the function of this region ... | 1992 | 1400230 |
| the virulence gene activator toxt from vibrio cholerae is a member of the arac family of transcriptional activators. | virulence gene expression in vibrio cholerae is postulated to involve toxr-dependent activation of the toxt gene followed by toxt activation of virulence genes, including several of those involved in biogenesis of the toxin-coregulated pilus. toxr is a transmembrane, dna-binding protein which is a member of the ompr subclass of two-component activator systems in bacteria. data presented in this report demonstrate that toxt is similar to the arac family of transcriptional activators identified in ... | 1992 | 1400247 |
| specific detection of campylobacter jejuni and campylobacter coli by using polymerase chain reaction. | development of a routine detection assay for campylobacter jejuni and campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction (pcr). an oligonucleotide primer pair from a conserved 5' region of the flaa gene of c. coli vc167 was used to amplify a 450-bp region by pcr. the primer pair specifically detected 4 strains of c. coli and 47 strains of c. jejuni; but it did not detect strains of campylobacter fetus, campylobacter lari, campylobacter upsaliensis, cam ... | 1992 | 1400961 |
| vibrio-associated gastroenteritis in the lower cross-river basin of nigeria. | a total of 120 vibrio species were isolated from 588 patients with acute diarrheal disease during an outbreak of gastrointestinal tract infections at different locations in the lower cross river basin of nigeria. vibrio cholerae o1, biotype el tor, serotype ogawa, was the prominent organism isolated from the vibrio-associated diarrheal cases. during the 3 months of study, v. cholerae non-o1 was recovered from 10 patients while vibrio parahaemolyticus was recovered from 19 patients. the significa ... | 1992 | 1400976 |
| development and testing of a nonradioactive dna oligonucleotide probe that is specific for vibrio cholerae cholera toxin. | an alkaline phosphatase-labeled oligonucleotide dna probe (ctap) that was specific for the cholera toxin gene (ctxa) was identified. all cholera toxin-producing strains of vibrio cholerae, regardless of serotype, hybridized with the ctap probe, while nontoxigenic strains from either environmental sources or from deletion or substitution mutations did not hybridize. unlike the whole-gene probes for either ctxa or for the heat-labile toxin or escherichia coli (elta), this 23-base sequence did not ... | 1992 | 1400994 |
| evidence that inactivated oral cholera vaccines both prevent and mitigate vibrio cholerae o1 infections in a cholera-endemic area. | in a randomized, placebo-controlled field trial of b subunit-killed whole cell (bs-wc) and killed whole cell only (wc) inactivated oral cholera vaccines in rural bangladesh, active surveillance of selected neighborhoods during the first year after vaccination identified 127 vibrio cholerae o1 infections among 3285 three-dose recipients. for each vaccine, protective efficacy was greater against symptomatic (57%, p < .05 for bs-wc; 58%, p < .05 for wc) than against asymptomatic infections (46%, p ... | 1992 | 1402014 |
| [in vitro antimicrobial activity of dr-3355, a new quinolone antibacterial agent, against clinical isolates of enteritis-causing bacteria]. | we determined the minimum inhibitory concentration (mic) of dr-3355, a newly developed quinolone-derivative antibacterial agent, against clinical isolates of various bacterial species from enteritis patients, and compared them with those of ofloxacin (oflx), ciprofloxacin (cpfx), nalidixic acid (na), ampicillin (abpc), kanamycin (km). mic90 of dr-3355 against 94 strains of shigella spp. and 5 strains of escherichia coli, 36 strains of salmonella spp., 22 strains of vibrio cholerae, 5 strains of ... | 1992 | 1402063 |
| [a case of vibrio cholerae non-o1 septicemia with liver cirrhosis]. | a case of vibrio cholerae non-o1 septicemia is described in this paper. a 45-year-old male with a three year history of liver cirrhosis, was admitted to our division with hematemesis, abdominal pain, high fever and a loss of consciousness. three days before onset of symptoms, he traveled to ishigaki island and ate a raw lobster. two days after, his temperature rose to 39.7 degrees c and the blood pressure dropped to 36/- mmhg. by endoscopic examination, an ulcer was found in the stomach, and the ... | 1992 | 1402101 |
| block of voltage-gated potassium currents by anticonvulsant u-54494a in mouse neuroblastoma cells. | u-54494a [(+-)-cis-3,4-dichloro-n-methyl-n-[2-(1- pyrrolidinyl)-cyclohexyl]-benzamide], an anticonvulsant and na channel blocker, was examined for its interaction with delayed rectifier k channels in mouse neuroblastoma cells (nie-115) using whole-cell and inside-out configurations of the patch clamp techniques. u-54494a at anticonvulsant doses reversibly blocked the tetraethylammonium-sensitive outward k current in a time-dependent manner without affecting the current-voltage relationship. anal ... | 1992 | 1403785 |
| [development and application of polymerase chain reaction (pcr) and dna hybridization for detection of cholera enterotoxin-producing v. cholerae and other pathogens]. | 1992 | 1404923 | |
| characterization of a vibrio cholerae virulence factor homologous to the family of tonb-dependent proteins. | irga is an iron-regulated virulence factor for infection in an animal model with classical vibrio cholerae strain 0395. we detected gene sequences hybridizing to irga at high stringency in clinical isolates in addition to 0395, including another classical strain of v. cholerae, three v. cholerae strains of the el tor biotype, three non-o1 isolates of v. cholerae, and individual isolates of vibrio parahaemolyticus, vibrio fluvialis, and vibrio alginolyticus. no hybridization to irga was seen with ... | 1992 | 1406279 |
| a simple purification method of vibrio cholerae non-o1 hemagglutinin/protease by immunoaffinity column chromatography using a monoclonal antibody. | a new simple purification method (i) for vibrio cholerae non-o1 hemagglutinin/protease (nag-ha/p) was developed. the method (i) requires only an immunoaffinity column chromatography using a monoclonal antibody against nag-ha/p. the method (i) is much simpler than previously reported purification method (ii) (honda, t. et al, infection and immunity 57: 2799-2803, 1989) which required four or more complicated chromatographic procedures. method (i) also gave an improved recovery rate (about 27%) co ... | 1992 | 1406368 |
| emr, an escherichia coli locus for multidrug resistance. | an escherichia coli chromosomal dna fragment cloned on a multicopy plasmid conferred resistance to carbonylcyanide m-chlorophenylhydrazone, nalidixic acid, and a number of other toxic compounds. the sequence of the cloned emr locus located at minute 57.5 of the chromosome revealed two open reading frames, emra and emrb. emrb encodes a highly hydrophobic 56.2-kda peptide, with 14 potential alpha-helices to span the inner membrane. the peptide is homologous to qaca, a multidrug-resistant pump from ... | 1992 | 1409590 |
| [the enzymatic activity of ammonia metabolism in the liver during the parenteral nitrogen feeding of animals with experimental liver failure]. | it was established that liver insufficiency the activity of hepatic glutamate dehydrogenase is markedly reduced that is, apparently, the main cause of hyperammonemia that accompanies hepatic encephalopathy. here occurs a significant reduction of the processes of ammonia detoxication with formation of glutamine in the liver as evidenced by changes of the activity of glutamine synthetase. administration parenterally of a preparation of nitrous feeding increases essentially ammonia detoxicating fun ... | 1992 | 1413686 |
| [the fatty acid composition of the total lipids in vibrio cholerae]. | the fatty acid composition of total cell lipids of v. albensis typing strains, serovars 01-012, isolated from patients and environmental objects, as well as v. eltor and nag vibrios, has been studied. fatty acids contain 14-18 carbon atoms and are mainly represented by unsaturated monoacids. palmitoleic and oleic acids constitute the greater part of unsaturated acids (their total content is 55.2-71.0%). the level of saturated fatty acids in considerably lower (27.0-43.1%), and these acids are ma ... | 1992 | 1414098 |
| elisa for seroepidemiological study of exposure to vibrio cholerae of population in krabi province, thailand. | seroepidemiological study of vibrio cholerae exposure was carried out in krabi province during january 1989 to december 1990 using indirect elisa to determine serum antibodies to lipopolysaccharides (lps) of v. cholerae. among 363 serum samples obtained from cord blood and venous blood of healthy persons, aged from 6 months to over 50 years, 65% and 64% were found positive for specific igg and igm against lps of v. cholerae, respectively. the seroprevalence of v. cholerae infection increased wit ... | 1992 | 1418185 |
| an n-hydroxylated derivative of cyanamide that inhibits yeast aldehyde dehydrogenase. | a stable, n,o-dibenzoyl derivative (dbhc) of n-hydroxycyanamide, the latter the postulated bioactivation product of the alcohol deterrent agent, cyanamide, has been synthesized. dbhc was an effective inhibitor of yeast aldehyde dehydrogenase (aidh) in vitro and inhibited this enzyme in a concentration-dependent manner with an ic50 of 25 microm. hydrolysis of the benzoate moiety of dbhc with dilute naoh gave rise to the formation of nitroxyl (hn = o), detected by gas chromatography as nitrous oxi ... | 1992 | 1418657 |
| acquired immunity to larvae of amblyomma marmoreum and a.hebraeum by tortoises, guinea-pigs and guinea-fowl. | a study of acquired resistance in guinea-pigs, guinea-fowl and tortoises to larvae of the ticks amblyomma hebraeum koch and a.marmoreum koch (acari: ixodidae) showed that repeated infestations of the laboratory host resulted in a 29.3-49.4% reduction respectively in weight of engorged larvae. no resistance was induced in guinea-fowl, the natural host for larvae of both species. a small decline in the mean weight of engorged larvae was observed for both species of ticks after three successive inf ... | 1992 | 1421505 |
| an evaluation of the sat in the diagnosis of typhoid. | salmonella typhi o and h antibody titres were determined by the standard agglutination test (sat) in 85 patients with bacteriologically proven typhoid, 102 patients with non-typhoidal febrile illnesses (control group 1), and 170 healthy subjects (control group 2). an o antibody titre of 1/120 occurred in 93% of patients with typhoid fever (7% false negatives), and in 17% and 12% control groups 1 and 2 respectively. an h antibody titre of 1/120 was found only in 64% of patients with typhoid (fals ... | 1992 | 1423751 |
| cellulose acetate electrophoretic determination of bone alkaline phosphatase activity in healthy subjects and diabetic patients with and without osteopenia. | we adapted the electrophoretic method of bone alkaline phosphatase (alp) determination using neuraminidase from vibrio cholerae to separate bone and liver alp on cellulose acetate membrane. treatment of separator plus serum (1:8, neuraminidase 111 u/l in final) for 10 min at room temperature (25 +/- 1 degree c) and subsequent electrophoresis made it possible to quantify bone alp activity simply and rapidly. the precision of the data was at the level of cv of 1.6% (within-day) and 4.7% (day-to-da ... | 1992 | 1424153 |
| the protease from vibrio cholerae nicks arginine at position 192 from the n-terminus of the heat-labile enterotoxin a subunit from enterotoxigenic escherichia coli. | it was examined where a protease purified from vibrio cholerae might nick the heat-labile enterotoxin (lt) a subunit from enterotoxigenic escherichia coli. lt was digested by the protease and contained a fragment which had the same mobility on sds-page as that of the a1 fragment of lt digested by trypsin. the biological activity of lt by this protease was also identical to that of lt by trypsin. the amino acid sequence of the n-terminus of the a2-like fragment was thr-ser-thr-gly, which correspo ... | 1992 | 1426177 |
| the second cholera toxin, zot, and its plasmid-encoded and phage-encoded homologues constitute a group of putative atpases with an altered purine ntp-binding motif. | it is shown that the second cholera toxin, zot, orf3 product of pseudomonas plasmid pkb740, and orf424 product of bacteriophage pf1 are a group of closely related proteins containing a modified version of the purine ntp-binding motif, with a drastic substitution of tyrosine for a conserved glycine. they are distantly but reliably related to the product of gene i of filamentous bacteriophages which is a putative atpase containing the classical ntp-binding motif and is involved in bacteriophage as ... | 1992 | 1426234 |
| reconstitution and kinetics of vacuolar h(+)-translocating atpases from plants and animals. | 1992 | 1426540 | |
| neuronal expression of the tissue specific splicing protein smn and its possible role in pre mrna splicing. | 1992 | 1426556 | |
| studies on the mechanism of the inhibition of lipogenesis by growth hormone in sheep adipose tissue. | 1992 | 1426557 | |
| the regulation of microsomal glucose-6-phosphatase differs between liver and kidney. | 1992 | 1426558 | |
| purification and characterization of a feruloyl/p-coumaroyl esterase from solid-state cultures of the aerobic fungus penicillium pinophilum. | 1992 | 1426561 | |
| effect of lipid peroxidation on molecular arrangement of phospholipids in liposomes prepared from egg yolk phosphatidylcholine or total rat brain lipids. a 31p nmr study. | changes in molecular arrangement of membrane phospholipids in the course of lipid autoxidation were studied by means of broad-band 31-p nmr spectroscopy. multilamellar liposomes prepared from egg yolk phosphatidylcholine (pc) or total lipid extracts from rat brains (tl) were used as models. the initial lamellar arrangement of phospholipids of both types changed as lipid peroxidation proceeded and a narrow isotropic signal appeared in the spectra at 0 ppm, this phenomenon being more prominent for ... | 1992 | 1426979 |
| the nysb sund double mutant of dictyostelium discoideum is blocked in the acquisition of non-degradative resistance to the pea phytoalexin pisatin. | dictyostelium discoideum amoebae can be induced to acquire a non-degradative resistance to the pea phytoalexin pisatin. the nysb sund double mutant is blocked in the acquisition of this resistance. using parasexual genetics it has been shown that sund is a recessive mutation linked to nysb on linkage group vi. | 1992 | 1426988 |
| development and evaluation of a rapid, simple, sensitive, monoclonal antibody-based co-agglutination test for direct detection of vibrio cholerae 01. | cholera epidemics caused by vibrio cholerae 01 continue to represent a major public health concern in many developing countries. a rapid and simple test kit for the detection of v. cholerae 01 has been developed. the kit, cholerascreen is a monoclonal antibody-based, co-agglutination test and is used directly with stool specimens. it does not include culturing the specimen and is performed without the need for sophisticated laboratory equipment. specificity of the test was demonstrated, using 11 ... | 1992 | 1427010 |
| experience with a measles vaccine manufactured in india. | five hundred and twenty seven children between 7 months and 2 years of age were vaccinated with measles vaccine manufactured by the serum institute of india. the sero-conversion rate in children who had no antibodies previous to vaccination was 98.4% as tested in hi. ninety per cent of children who had pre-vaccination measles antibodies showed a two-fold or more rise in hi antibodies. the side reactions of the vaccine were negligible. | 1992 | 1428138 |
| chloramphenicol resistant s. typhi. | 1992 | 1428156 | |
| novel surface molecules involved in human nk cell activation and triggering of the lytic machinery. | three new monoclonal antibodies (mabs) termed 7a6, pp35 and a6/143 were isolated after mouse immunization with cd3- cd16+ nk clones. the screening procedure was based on the ability of mabs to trigger cytolytic activity of the immunizing clones in a re-directed killing assay against the p815 murine mastocytoma cell line. the 7a6 mab reacts with 58 kda surface molecules that appear to belong to the same molecular family defined by the previously described nk-sub-set-specific gl183 and eb6 mabs. h ... | 1992 | 1428407 |
| a non-invasive technique for the accurate measurement of leg length in animals. | a novel, non-invasive technique for accurate measurements in animals (kyniklometry) is presented. kyniklometry (derived from greek ŏ kúvikloz the rabbit) determines the distance between soft tissue landmarks in conscious rabbits, in particular the rear lower leg. each measurement consists of six subsequent and independent estimations of this distance, with a technical error of 79 microns (study i), respectively 83 microns (study ii). the angle of the relaxed sitting animal's knee is approximatel ... | 1992 | 1428414 |
| expression of transforming growth factor-beta (tgf-beta 1) and insulin-like growth factor ii (igf-ii) messenger rna in the developing subcutaneous tissue (sq) of the fetal pig. | studies (in situ hybridization) were performed on the developing subcutaneous tissue (sq) of the pig fetus to determine sites of synthesis of tgf-beta 1 and igf-ii. tissues from 50, 70, 90, 110-day-old pig fetuses and 7-day-old postnatal pigs were bouin's fixed, paraffin embedded and hybridized with biotin labelled cdna probes. the expression of tgf-beta 1 messenger rna was detectable primarily in the dermis, hair follicle fat lobule, outer and inner sq areas at all ages. cells adjacent to adipo ... | 1992 | 1428415 |
| candida albicans--saprophyte or pathogen? | skin scrapings taken from toe spaces of 200 healthy volunteers and from toe webs and groins of 150 pediatric patients were cultured for candida albicans using the serum germ-tube test. the results showed that candida albicans can be isolated in about 15% of normal toe spaces and 14% of children with normal groins. although candida albicans can be found in various grades of athlete's foot and also in some abnormal groins, we believe that it is not necessarily responsible for these conditions and ... | 1992 | 1428429 |
| cloning of a metalloprotease gene involved in the virulence mechanism of vibrio anguillarum. | genetic evidence has previously suggested that a zinc metalloprotease is involved in the invasive mechanism of the fish pathogen vibrio anguillarum nb10. in this study, the metalloprotease gene was cloned and sequenced. the sequence encodes a polypeptide (611 amino acids) that contains a putative signal sequence followed by a large leader sequence and the mature protein (44.6 kda). since the purified protein has a molecular mass of 36 kda instead of the predicted 44.6 kda, the mature protein is ... | 1992 | 1429449 |
| acute secretory travellers' diarrhoea caused by vibrio cholerae non-01 which does not produce cholera-like or heat-stable enterotoxins. | an australian tourist suffering from severe acute watery diarrhoea and dehydration due to vibrio cholerae non-01 was studied. the v. cholerae strain isolated from the patient belonged to serovar 05. the organism did not produce any of the conventional enterotoxins including cholera-toxin (ct) or heat-stable toxins (nag-st) that are known to be associated with intestinal secretion. this report suggests that toxin(s) other than ct-like or nag-st may be involved in the pathogenesis of diarrhoea by ... | 1992 | 1430972 |
| cholera in piura, peru: a modern urban epidemic. | in late january 1991, epidemic cholera appeared in peru. within 2 months, 7922 cases and 17 deaths occurred in piura, a peruvian city of 361,868. a hospital-based culture survey showed that 79%-86% of diarrhea cases were cholera. high vibriocidal antibody titers were detected in 34% of the asymptomatic population. a study of 50 case-patients and 100 matched controls demonstrated that cholera was associated with drinking unboiled water (odds ratio [or], 3.9; 95% confidence interval [ci], 1.7-8.9) ... | 1992 | 1431259 |
| outbreak of cholera associated with crab brought from an area with epidemic disease. | from 31 march through 3 april 1991, 8 new jersey residents developed severe, watery diarrhea after eating crabmeat brought back in the suitcase of a traveler to ecuador. stool cultures yielded toxigenic vibrio cholerae o1, serotype inaba, biotype el tor from 4 persons, and vibriocidal antibody titers were > or = 1:640 in 7 persons, indicating recent infection with vibrio cholerae o1. eating crab was statistically associated with illness (p = .006); however, no leftover crabmeat was available for ... | 1992 | 1431260 |
| increases in extracellular dopamine in the nucleus accumbens by cocaine are inversely related to basal levels: effects of acute and repeated administration. | repeated administration of cocaine enhances several of the behavioral and neurochemical responses to subsequent cocaine injections, an effect that has been attributed, in part, to decreased somatodendritic autoreceptor sensitivity of mesocorticolimbic dopamine (da) neurons. such changes in autoregulation may not only modify the direct effects of cocaine on extracellular da levels but also result in tonically increased basal da release in the terminal areas of the mesocorticolimbic da system. the ... | 1992 | 1432099 |
| impact of zinc supplementation on intestinal permeability in bangladeshi children with acute diarrhoea and persistent diarrhoea syndrome. | zinc has been shown to enhance intestinal mucosal repair in patients suffering from acrodermatitis enteropathica; but the impact on mucosal integrity during acute (ad) or persistent (pd) diarrhoea is unknown. one hundred eleven children with ad and 190 with pd aged between 3 and 24 months received, randomly and blind to the investigators, either an elemental zinc supplement of 5 mg/kg body wt/day or placebo in multivitamin syrup for 2 weeks while intestinal permeability and, biochemical and anth ... | 1992 | 1432467 |
| [effect of ph on preferential antibacterial-activity of ethylenediaminetetraacetic acid (edta)]. | ethylenediaminetetraacetic acid (edta), a chelating agent, was examined for the antibacterial activity against 15 species of bacteria by treating with a 10mm solution at ph adjusted to 5.0, 7.0 or 9.0. all bacterial species tested were classified into three groups; tentatively named the ph5 edta-sensitive group comprising vibrio cholerae and staphylococcus aureus, the ph9 edta-sensitive group comprising escherichia coli and pseudomonas aeruginosa and the edta-nonsensitive group comprising proteu ... | 1992 | 1433911 |
| kinetic studies of the mechanism of thrombocytopenia in patients with human immunodeficiency virus infection. | isolated thrombocytopenia accompanied by increased amounts of platelet-associated antibody is a common manifestation of human immunodeficiency virus (hiv) infection, and the thrombocytopenia often improves with zidovudine. it is not clear whether the mechanism of hiv-related thrombocytopenia primarily involves autoimmune destruction of platelets or reduced platelet production by megakaryocytes. | 1992 | 1435932 |
| a periplasmic protein disulfide oxidoreductase is required for transformation of haemophilus influenzae rd. | the mutated gene in jg16, a haemophilus influenzae strain deficient in competence-induced dna binding and uptake, was cloned and the wild-type allele was sequenced. the gene was shown by northern analysis to be constitutively expressed on a 1.7-kilobase transcript. the gene product was identified as a 20.6-kda protein targeted to the periplasm. the protein contains the sequence cys-pro-his-cys (cphc) and is highly similar to two other periplasmic cphc motif-containing proteins: dsba, an escheric ... | 1992 | 1438213 |
| the mapping by high-ph anion-exchange chromatography with pulsed amperometric detection and capillary electrophoresis of the carbohydrate moieties of human plasma alpha 1-acid glycoprotein. | the reducing oligosaccharides released from alpha 1-acid glycoprotein (agp) by conventional hydrazinolysis have been analyzed by two different mapping techniques, using high-ph anion-exchange chromatography with pulsed amperometric detection (hpae-pad) and capillary electrophoresis (ce) with uv detection at 190 nm. the ce measurements proved about 4000 times more sensitive than the measurements by hpae-pad. the n-glycan pool was fractionated by mono q anion-exchange chromatography, and individua ... | 1992 | 1443615 |
| induction of melanin biosynthesis in vibrio cholerae. | vibrio cholerae synthesized the pigment melanin in response to specific physiological conditions that were stressful to the bacterium. pigmentation was induced when v. cholerae was subjected to hyperosmotic stress in conjunction with elevated growth temperatures (above 30 degrees c). the salt concentration tolerated by v. cholerae was lowered by additional abiotic factors such as acidic starting ph of the growth medium and limitation of organic nutrients. although the amount of toxin detected in ... | 1992 | 1444398 |
| cloning and sequencing of a human cdna coding for dihydroorotate dehydrogenase by complementation of the corresponding yeast mutant. | dihydroorotate dehydrogenase (dhodehase, ec 1.3.3.1) catalyses the fourth enzymatic step in de novo pyrimidine biosynthesis. a truncated human cdna encoding this enzyme was isolated from a hela cell cdna library by functional complementation of a corresponding deletion mutant from the yeast, saccharomyces cerevisiae. the complementing clone contained a 1.5-kb poly(a)(+)-tailed insert with a 1191-bp open reading frame, hybridising with a unique human mrna of 1.6 kb. the deduced amino acid sequenc ... | 1992 | 1446837 |
| ventilation is stimulated by small reductions in arterial pressure in the awake dog. | changes in arterial pressure commonly accompany respiratory adaptations. the purpose of this study was to determine, in awake dogs (n = 6), the degree to which small acute decreases in arterial pressure affect ventilation and acid-base balance. mean arterial pressure (map) was reduced by 6 +/- 2, 10 +/- 3, and 16 +/- 2% by intravenous infusion of sodium nitroprusside for sequential 20-min periods. in another experiment, the ventilatory response to hypercapnia was determined during map reduction ... | 1992 | 1447103 |
| [characteristics of the dynamics of hematopoietic precursor cells cloned in diffusion chambers and recorded in experiments on mice and dogs irradiated in median lethal doses]. | in experiments with mice and dogs irradiated with ld50, it was shown the postirradiation depopulation of haemopoietic polypotent (cfus) cell-precursors in mouse bone marrow was more pronounced than that of granulocytic and macrophagal cells (cfudc). the rate of repopulation of cfus during the first week was higher than that of cfudc (t1/2 was 2.5 and 8.8 days respectively). in dogs, one could notice a partial change in the colony formation, a prolonged plateau period in the postirradiation cfudc ... | 1992 | 1448562 |
| [pathomorphology of acute and chronic stages of ccl4-induced liver fibrosis: immunohistochemical and in situ hybridization studies]. | the cellular localization of expression of various genes activated during the course of liver fibrosis and regeneration was studied by immunohistology and in situ hybridization in rat and human liver tissues. mesenchymal cells proved to be the principal sources of extracellular matrix proteins and of fibrogenic growth factors, whereas the collagenase-activating protease transin/stromelysin gene was transcribed in parenchymal cells as well. fibrogenesis by the mesenchymal compartment appears to b ... | 1992 | 1449012 |
| molecular correlates of physiological regulation in a p-type h(+)-atpase. | 1992 | 1449060 | |
| cloning and expression in escherichia coli of the gene encoding an extracellular deoxyribonuclease (dnase) from aeromonas hydrophila. | the gene encoding an extracellular dnase from aeromonas hydrophila chc-1 has been cloned and sequenced. following expression of the dns in escherichia coli, it was revealed that some of the cloned enzyme was present in the cell-free extracellular supernatant fluid, and there was no cell lysis and concurrent release of cytoplasmic or periplasmic proteins. therefore, results suggest that e. coli cells were capable of secreting the dnase extracellularly, albeit very inefficiently. the dns is transc ... | 1992 | 1452026 |
| dna sequence determination and functional characterization of the oct-plasmid-encoded alkjkl genes of pseudomonas oleovorans. | the alkbfghjkl and alkst operons encode enzymes that allow pseudomonas putida (oleovorans) to metabolize alkanes. in this paper we report the nucleotide sequence of a 4592 bp region of the alkbfghjkl operon encoding the alkj, alkk and alkl polypeptides. the alkj gene encodes a protein of 59 kilodaltons. the predicted amino acid sequence shows significant homology with four flavin proteins: choline dehydrogenase, a glucose dehydrogenase and two oxidases. alkj is membrane-bound and converts alipha ... | 1992 | 1453953 |
| [the comparative characteristics of vibrio cholerae hemolysins]. | biochemical and biological properties of in vivo and in vitro hemolysins of cholera germs isolated by different authors as well as hemolysins of vibrio parahaemolyticus and escherichia coli have been comparatively characterized. according to the above data hemolysin of cholera germ el tor is a thermolabile protein with molecular mass of 60-80 thou.; it is cytotoxic, enteropathogenic and lyses all the species of erythrocytes. hemolysins of v. eltor and germs are immunologically related. hemolysin ... | 1992 | 1453988 |
| distribution of 5-ht1 binding sites in cat spinal cord. | quantitative analysis of high affinity [3h]5-ht binding to 5-ht1 receptors in the cervical, thoracic, lumbar, and sacral spinal cord of the cat revealed specific binding throughout the grey matter, with the highest levels of binding in laminae ii and iii, and the lowest levels in laminae i and vii. relatively high levels were also observed in the thoracic intermediolateral cell column. there were no significant differences in the degree of binding between various segmental levels. comparison of ... | 1992 | 1454202 |
| salmonella and vibrio cholerae in brackishwater cultured tropical prawns. | the occurrence of salmonella and vibrio cholerae in brackishwater ponds was monitored over a 2-year period in one of the major prawn exporting countries in southeast asia. the principal production areas were identified and regular samples taken for salmonella and v. cholerae analysis. results demonstrated that brackishwater ponds and cultured prawns were inherently contaminated with both bacterial pathogens. salmonella spp. were present in 16.0% of prawns and 22.1% of mud/water samples from pond ... | 1992 | 1457289 |
| structure of the n- and o-glycans of the a-chain of human plasma alpha 2hs-glycoprotein as deduced from the chemical compositions of the derivatives prepared by stepwise degradation with exoglycosidases. | the structure of the glycans of the a-chain of human plasma alpha 2hs-glycoprotein was established from the chemical compositions of its derivatives prepared by sequential enzymatic degradation of the carbohydrate moiety, from the determination of the kind and amount of the monosaccharides liberated after each step of the enzymatic digestion, and from the distinct specificity of the highly purified exoglycosidases. the exoglycosidases were three sialidases (vibrio cholerae, fowl plague virus, an ... | 1992 | 1457416 |
| bacterial lipopolysaccharide mediates the loss of cd4 from the surface of purified peripheral blood monocytes. | in this study we have looked at the effect of lipopolysaccharide (lps) on the surface antigen expression of cultured monocytes. monocytes were purified from peripheral blood mononuclear cells (pbmc) and cultured in the presence or absence of lps. the cultured cells were then stained with anti-mo3, anti-il-2r and anti-cd4 moabs. we have shown that freshly isolated monocytes are il-2r- and mo3-negative and express cd4 in low density. after overnight culture, without lps, the expression of these su ... | 1992 | 1458690 |
| [incidence, frequency and resistance characteristics of methicillin-oxacillin resistant staphylococcus aureus strains in germany]. | in a multicentre study, the methicillin-resistant staphylococcus aureus (mrsa) isolates in 19 large clinics in germany were recorded, and the resistance characteristics of these strains were studied. oxacillin-mannitol-salt agar plates were distributed to all participants to ensure uniformity of screening, and each laboratory used these plates to investigate 200 consecutive staphylococcus aureus isolates for oxacillin-methicillin resistance. of the 3,794 evaluable staphylococcus aureus isolates, ... | 1992 | 1459021 |
| characterization of two forms of hemagglutinin/protease produced by vibrio cholerae non-o1. | two forms (34 kda and 32 kda) of hemagglutinin/protease produced by vibrio cholerae non-o1 were characterized. the hemagglutinin/protease purified by immunoaffinity column chromatography using a monoclonal antibody was essentially a 34-kda form. by incubation of the purified 34-kda form at 37 degrees c, it was processed (autodigested) to the 32-kda form. the n-terminal 20 amino acid sequences of both the 34- and 32-kda forms were identical, suggesting that proteolytic processing at the c-termina ... | 1992 | 1459409 |
| n-acetyl-d-glucosamine-specific lectin purified from vibrio cholerae 01. | an n-acetyl-d-glucosamine-specific cell associated hemagglutinin (ha) was isolated and purified from a strain of vibrio cholerae 01 by chitin affinity chromatography followed by separation on bio gel p-150. a single stained protein band of 47 kda in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) was observed with the purified ha. ha-antisera produced a single precipitin band against the purified ha in an immunodiffusion test without exhibiting any reactivity towards purifie ... | 1992 | 1459412 |
| [brucella infection of the testis mimicking malignancy]. | a testicular mass is a diagnostic challenge, as it may include both benign and malignant processes. due to the difficulty in diagnosis and the need to prevent complications, it is sometimes necessary to perform orchiectomy. we present an unusual case of brucellosis in which the presenting features were persistent cough for 6 months and a right testicular mass. the clinical picture and ultrasound findings suggested malignancy. orchiectomy and medical treatment resulted in rapid resolution of symp ... | 1992 | 1459502 |
| should cow milk be diluted for young infants. | 1992 | 1459690 | |
| acquired malaria--how early can it occur. | 1992 | 1459693 | |
| evidence for an s-layer protein pool in the peptidoglycan of bacillus stearothermophilus. | intact cells of bacillus stearothermophilus pv72 revealed, after conventional thin-sectioning procedures, the typical cell wall profile of s-layer-carrying gram-positive eubacteria consisting of a ca. 10-nm-thick peptidoglycan-containing layer and a ca. 10-nm-thick s layer. cell wall preparations obtained by breaking the cells and removing the cytoplasmic membrane by treatment with triton x-100 revealed a triple-layer structure, with an additional s layer on the inner surface of the peptidoglyca ... | 1992 | 1459950 |
| pathogenesis and ecology: the case of cholera. | the way in which the results of various studies of cholera have influenced the understanding of the disease are discussed. attention is given to the outcomes of medical research, targeted on problems of cholera and biological research focused on understanding vibrio cholerae. the contrast between these approaches has produced a new understanding of the ecological nature of disease. the importance of the environmental reservoir of v. cholerae as the engine for the generation of diversity is highl ... | 1992 | 1460695 |
| the protective activity of tea catechins against experimental infection by vibrio cholerae o1. | tea catechins inhibited the fluid accumulation induced by cholera toxin in sealed adult mice. the catechins also reduced fluid accumulation by vibrio cholerae o1 in ligated intestinal loops of rabbits. these findings suggest that tea catechins may possess protective activity against v. cholerae o1. | 1992 | 1461156 |