Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| crystal structure of cholera toxin b-pentamer bound to receptor gm1 pentasaccharide. | cholera toxin (ct) is an ab5 hexameric protein responsible for the symptoms produced by vibrio cholerae infection. in the first step of cell intoxication, the b-pentamer of the toxin binds specifically to the branched pentasaccharide moiety of ganglioside gm1 on the surface of target human intestinal epithelial cells. we present here the crystal structure of the cholera toxin b-pentamer complexed with the gm1 pentasaccharide. each receptor binding site on the toxin is found to lie primarily with ... | 1994 | 8003954 |
| [imported cholera infection caused by a new nonagglutinating cholera agent]. | within 24 hours of returning from a five-week holiday in pakistan a 15-year-old girl developed vomiting and massive diarrhoea leading to severe dehydration with hypovolaemic shock. the diastolic blood pressure was no longer measurable and prerenal renal failure occurred with a serum creatinine of 4.4 mg/dl and metabolic acidosis (ph 7.21, base excess-16.9 mmol). initially treatment consisted of rehydration (day 1: 9280 ml, day 2: 4850 ml). the patient's condition rapidly improved and she had vol ... | 1994 | 8005066 |
| an epidemic of cholera due to vibrio cholerae o139 in dhaka, bangladesh: clinical and epidemiological features. | we describe the disease spectrum and socio-demographic and epidemiological features of an epidemic of cholera due to a new pathogen, vibrio cholerae o139, in patients attending a very large hospital in the metropolitan city of dhaka, bangladesh. this hospital treats 70,000-90,000 patients a year with diarrhoeal diseases. a 4% systematic sample of 1854 patients attending from january to april 1993 were studied. five hundred and two (27%) of the 1854 patients were culture positive for v. cholerae ... | 1994 | 8005212 |
| the vibrio cholerae toxin-coregulated-pilus gene tcpi encodes a homolog of methyl-accepting chemotaxis proteins. | virulence gene activation in vibrio cholerae is under the control of the toxr-toxt regulatory cascade. the toxr regulon consists of genes required for toxin-coregulated-pilus (tcp) biogenesis, accessory colonization factor genes, cholera toxin genes, and toxr-activated genes (tag) of unknown function. the tagb gene was isolated by using a tagb::tnphoa fusion junction to probe a v. cholerae )395 bacteriophage lambda library. nucleotide sequence analysis revealed that tagb is identical to tcpi, a ... | 1994 | 8005659 |
| food protection activities of the pan american health organization. | one of the most widespread health problems in the caribbean and latin america is contaminated food and foodborne illness. the pan american health organization (paho) has been a major force in activities to strengthen food protection. the program within the regional program of technical cooperation is administered by the veterinary public health program and under the guidance of the pan american institute for food protection and zoonoses in buenos aires, argentina. a food action plan for 1986- ... | 1994 | 8012436 |
| production of dogik: an improved ogi (nigerian fermented weaning food) with potentials for use in diarrhoea control. | as part of a programme to formulate foods to aid the control of diarrhoeal diseases, an improved ogi (the commonest weaning food in nigeria) named dogik has been developed. dogik was produced by using lactobacillus starter cultures with antimicrobial activity against diarrhoeagenic bacteria and also possessing amylolytic activity. the survival of diarrhoeagenic bacteria was investigated in locally-fermented ogi and in dogik. the foods were inoculated with cell suspensions of salmonella, shigella ... | 1994 | 8015024 |
| [the characteristic properties of vibrio cholerae eltor isolated from environmental objects on the territory of the former ussr during the 7th cholera pandemic]. | the properties of 22,382 v. eltor strains isolated from environmental objects on the territory of different climatic and geographical zones during the period of 1970-1988 were studied. the study was made on the morphology of their colonies, the agglutinability of the strains by cholera o serum, type-specific serum and ro serum, their capacity for being lyzed by v. eltor bacteriophage, their hemolytic activity and virulence. differences in the occurrence of strains with any of the above-mentioned ... | 1994 | 8017114 |
| [genetic determination and the spectrum of the hemolytic activity of vibrio cholerae: their significance in the complex of biovar-specific traits]. | a collection of 363 v. cholerae strains isolated from different sources were studied by the spectrum of their hemolytic activity in combination with biovar-associated properties. the strains were analyzed for the presence of the cholera toxin (ct) gene (vct) and the hemolysin gene (hly) with the use of the ct probe and a previously cloned 6.56 kb fragment of v. eltor dna coding the synthesis of hemolysin. the study revealed that all v. cholerae strains had the hly gene irrespective of the spectr ... | 1994 | 8017123 |
| isolation of vibrio cholerae o139 synonym bengal from the aquatic environment in bangladesh: implications for disease transmission. | currently, bangladesh is experiencing an epidemic of acute watery diarrhea caused by vibrio cholerae o139. surface waters were collected and cultured for vibrious following enrichment. twelve percent (11 of 92) of samples yielded v. cholerae o139, and all of them were positive for cholera toxin. the data suggest that v. cholerae o139 is easily culturable from surface water samples. | 1994 | 8017948 |
| [the in vitro action of plants on vibrio cholerae]. | natural products of several plants, according to the geographic location, are used by peruvian people in the popular treatment of diarrhea, with good success. when cholerae cases appeared in peru, we were interested to know the "in vitro" effect against vibrio cholerae 01, of these useful plants to treat diarrhea. the following plants were tested: cichorium intybus, althaea officinalis, psorela glandulosa, geranium maculatum, punica granatum, malus sativa, cydonia oblonga, chenopodium ambrosoide ... | 1994 | 8018898 |
| reca mutations reduce adherence and colonization by classical and el tor strains of vibrio cholerae. | two reca mutants of vibrio cholerae (classical and el tor biotypes) were constructed by disruption of the wild-type reca gene with mutated reca sequences of v. cholerae cloned in the suicide vector pgp704. mutants defective in the reca gene were compared with their respective reca+ parent strains with regard to their adherence to isolated rabbit intestine and colonization of intestine of infant mice. the reca mutation in v. cholerae was found to diminish adherence and markedly affected colonizat ... | 1994 | 8025688 |
| detection of the vibrio cholerae heat-stable enterotoxin gene by polymerase chain reaction. | a polymerase chain reaction assay was developed for detection of the vibrio cholerae heat-stable enterotoxin gene (sto). the assay is based on two oligonucleotide primers suitable for amplification of the entire sto open reading frame. reaction conditions were defined to obtain optimal results in terms of specificity and sensitivity. under these conditions the assay was highly sensitive and enabled good results to be obtained using, as a template, crude dna preparations from single bacterial col ... | 1994 | 8028606 |
| [gastroenteritis with benign diarrhea caused by non-0:1 vibrio cholerae]. | 1994 | 8031897 | |
| emergence of a new cholera pandemic: molecular analysis of virulence determinants in vibrio cholerae o139 and development of a live vaccine prototype. | in october 1992, a non-o1 strain of vibrio cholerae emerged as a cause of epidemic cholera in india and bangladesh. this antigenically novel clone has been designated serogroup o139 synonym bengal. since its emergence, v. cholerae o139 has caused a massive cholera epidemic throughout and beyond the indian subcontinent. molecular analysis of virulence determinants in clinical isolates suggests that o139 strains are highly related to el tor o1 strains. unlike other non-o1 strains, o139 strains car ... | 1994 | 8035010 |
| the occurrence of non-01 vibrio cholerae in non-potable water samples in barbados. | fourteen freshwater or brackish-water samples taken from different sites were examined for the presence of vibrio cholerae. standard enrichment techniques, using pre-incubation in alkaline peptone water and plating on thiosulfate citrate bile sucrose agar (tcbs) followed by biochemical, physiological and morphological characterization of the isolates, revealed the presence of vibrio cholerae at nine of the sites examined. serotyping for type 01 only was performed. all the strains isolated were n ... | 1994 | 8036819 |
| [detection of convalescent vibrio cholerae carriers using the enterotest]. | although the existence of chronic carriers of vibrio cholerae has been posited, the information in this regard is limited and contradictory. in order to determine the usefulness of the encapsulated string test (enterotest) for detecting v. cholerae in duodenal secretions of biliary origin (biliduodenal secretions), 59 patients (30 males and 29 females) over the age of 15 with clinically and bacteriologically diagnosed cholera were evaluated. all the patients, who were treated at the maría auxili ... | 1994 | 8037848 |
| regioselective transglycosylation in the synthesis of oligosaccharides: comparison of beta-galactosidases and sialidases of various origins. | n-acetyl-lactosamine(beta-d-gal p-(1-->4)-d-glc pnac) was synthesized regioselectively with the aid of the transglycosylation activity of beta-galactosidase isolated from diplococcus pneumoniae using p-nitrophenyl beta-d-galactopyranoside as the donor. also, transglycosylation of the sialyl group in an alpha-(2-->8)-linked sialic acid dimer or p-nitrophenyl glycoside of sialic acid to n-acetyl-lactosamine was performed using sialidases of various origins. when sialidase from clostridium perfring ... | 1994 | 8039189 |
| adherence to human small intestines of capsulated vibrio cholerae o139. | capsulated cells of v. cholerae o139 adhered to formalin-fixed or native mucosa of the small intestines from an adult and a child. the primary adherence target was mucus. capsulated o139 cells adhered better to the antigen sampling cells (m cells) of ileal peyer's patch than to the absorptive cells. o139 cells on the mucosa appeared as small aggregates. similar organisms were found on the mucosa of duodenal biopsy samples from patients infected with v. cholerae o139. the findings indicated that ... | 1994 | 8039663 |
| construction and characterization of recombinant vibrio cholerae strains producing inactive cholera toxin analogs. | the catalytic a subunit of cholera toxin (ct-a) is capable of adp-ribosylating the guanine nucleotide-binding protein, which regulates cell adenylyl cyclase, leading to the life-threatening diarrhea of cholera. amino acids involved in the enzymatic activity of ct-a have previously been identified. by means of site-directed mutagenesis, an analog of the ct-a subunit gene was created with codon substitutions for both arg-7 and glu-112, each of which has been shown to produce subunits lacking adp-r ... | 1994 | 8039872 |
| the vibrio cholerae acfb colonization determinant encodes an inner membrane protein that is related to a family of signal-transducing proteins. | vibrio cholerae accessory colonization factor genes (acfa, b, c, and d) are required for efficient intestinal colonization. expression of acf genes is under the control of a regulatory cascade that also directs the synthesis of cholera toxin and proteins involved in the biogenesis of the toxin-coregulated pilus. the gene for acfb was cloned by using an acfb::tnphoa fusion junction to probe a v. cholerae o395 bacteriophage lambda library. dna sequence analysis revealed that acfb is predicted to e ... | 1994 | 8039900 |
| effect of rehydrating fluid 'electral' on vibrio cholerae cells. | v. cholerae ogawa 154 cells underwent rapid loss of colony forming capacity during the first few minutes' incubation in the electral medium at 37 degrees c, the turbidity of the suspension however increasing with time of incubation and leading to a plateau from 5 min onward. the vibrio suspension in the electral medium released small amounts of 280 nm and much higher amounts of 260 nm absorbing materials. on withdrawal of the electral medium, the cells underwent significant liquid holding recove ... | 1994 | 8045604 |
| a gene for the enterotoxin zonula occludens toxin is present in vibrio mimicus and vibrio cholerae o139. | the presence of the zonula occludens toxin (zot) gene, which encodes an enterotoxin produced by serotype o1 strains of the pathogenic bacterium, vibrio cholerae, in addition to cholera toxin, was investigated in selected strains of v. mimicus and the new pandemic v. cholerae non-o1 serotype o139. the zot gene was detected by polymerase chain reaction (pcr) amplification, using sets of primers based on the sequence of the v. cholerae o1 zot sequence. pcr amplification of genomic dnas of both chol ... | 1994 | 8050719 |
| vibrio cholerae fur mutations associated with loss of repressor activity: implications for the structural-functional relationships of fur. | we used the vibrio cholerae fur protein as a model of iron-sensitive repressor proteins in gram-negative bacteria. utilizing manganese mutagenesis, we isolated twelve independent mutations in v. cholerae fur that resulted in partial or complete loss of fur repressor function. the mutant fur genes were recovered by pcr and sequenced; 11 of the 12 contained point mutations (two of which were identical), and one contained a 7-bp insertion that resulted in premature truncation of fur. all of the mut ... | 1994 | 8051024 |
| direct detection of vibrio cholerae in stool samples. | a direct method to detect vibrio cholerae in stool samples was developed by using a pcr procedure that did not require a dna purification step. dilution (1/100) of stool samples prevented inhibition of the reaction by contaminants, and two consecutive pcrs, the second one with a nested primer, achieved the desired sensitivity. comparison of the results obtained from stool swab samples processed by the two-step pcr and by an enzyme-linked immunosorbent assay using gm1 as the capture molecule show ... | 1994 | 8051251 |
| [survival of vibrio cholerae o139 synonym bengal in water from a river]. | survival of five strains of vibrio cholerae, including serotypes o139 synonym bengal, o1 el tor, and non-o1 were compared in water from a river. these bacteria were mixed with water from a river and the water filtered through 0.45 micron millipore filters, respectively, to yield a concentration of 10(6) cfu/ml and incubated at 5 degrees c and 20 degrees c for 21 days. the survival curve of v. cholerae o139 was almost the same with v. cholerae o1 and non-o1. the number of these bacilli decreased ... | 1994 | 8051441 |
| colonization of professional divers by toxigenic vibrio cholerae o1 and v. cholerae non-o1 at dive sites in the united states, ukraine and russia. | vibrio cholerae, recognized as the causative agent of epidemic cholera, was isolated from healthy professional divers and from water samples collected at dive sites in the united states, ukraine and russia. swabs of nose, ear and throat of divers and their tank regulators, i.e. the divers and their diving gear, were taken before and after routine dives. blood samples were collected before and 30-60 days after each dive to measure igg and iga titers against the whole cell antigen of v. cholerae o ... | 1994 | 8056286 |
| cholera dfa: an improved direct fluorescent monoclonal antibody staining kit for rapid detection and enumeration of vibrio cholerae o1. | an improved fluorescent monoclonal antibody staining kit, cholera dfa, for direct detection and enumeration of vibrio cholerae o1 has been developed, employing a highly specific anti-a antigen monoclonal antibody, colta, labeled with fluorescein isothiocyanate (fitc). an optimized quantity of anti-photobleaching agent is used in a glycerol mounting medium to retard the rapid fading of immunofluorescent stained cells during fluorescent microscopy, thus enabling prolonged inspection of individual ... | 1994 | 8056287 |
| isolation of a vibrio cholerae transposon-mutant with an altered viable but nonculturable response. | we have isolated more than 2500 mutants of vibrio cholerae by using transposon mutagenesis. mutants were screened under low nutrient conditions in artificial seawater for an altered viable but nonculturable response, compared to the wild-type. mutant jr09h1 entered the viable but nonculturable state more rapidly than the wild-type at both 25 degrees c and 4 degrees c. | 1994 | 8056295 |
| animal models for immunoglobulin a secretion. | 1994 | 8057892 | |
| regulation of cholera toxin by temperature, ph, and osmolarity. | 1994 | 8057923 | |
| gene sequence of reca+ and construction of reca mutants of vibrio cholerae. | the reca+ gene of vibrio cholerae o1 has been cloned, its nucleotide sequence determined and the product characterized. a deletion mutation was constructed in the reca gene and mutants showed the typical sensitivity to uv and to dna-damaging agents, as well as an inability to mediate homologous dna recombination. the chromosomal reca deletion mutants in v. cholerae do not show altered virulence in the infant mouse cholera model and are thus ideal strains for use in complementation studies. | 1994 | 8058040 |
| scanning electron microscopy analysis of bacterial colonization in crustaceans. | 1994 | 8611268 | |
| analysis of the complexity of gene regulation by fur in vibrio cholerae. | iron concentration influences the expression of a number of genes involved in iron uptake and virulence in bacteria. in escherichia coli, coordinate regulation of these genes by iron depends on the product of the fur gene, which acts as an iron-responsive, dna-binding repressor protein. several genes in vibrio cholerae are also repressed by iron; and a fur gene, homologous to e. coli fur, has been previously cloned from this organism. the present study was undertaken to define the roles of fur a ... | 1994 | 8282702 |
| cholera epidemic among rwandan refugees: experience of icddr,b in goma, zaire. | in july 1994, one of the worst cholera epidemics broke out among the nearly a million rwandan refugees in goma, eastern zaire. the united nations high commission for refugees estimated that nearly 12,000 people died during the epidemic. the international centre for diarrhoeal disease research, bangladesh (icddr,b) sent an eight-member medical team to goma headed by dr ak siddique, a senior scientist of the center and head of the epidemic control preparedness program, dacca, bangladesh. during th ... | 1994 | 12288419 |
| transfer of multiple drug resistance plasmids between bacteria of diverse origins in natural microenvironments. | plasmids harboring multiple antimicrobial-resistance determinants (r plasmids) were transferred in simulated natural microenvironments from various bacterial pathogens of human, animal, or fish origin to susceptible strains isolated from a different ecological niche. r plasmids in a strain of the human pathogen vibrio cholerae o1 e1 tor and a bovine escherichia coli strain were conjugated to a susceptible strain of the fish pathogenic bacterium aeromonas salmonicida subsp. salmonicida in marine ... | 1994 | 11865872 |
| [the immunology of cholera and the molecular biology of cholera toxin. recent progress and future perspectives]. | vibrio cholerae has recently called the attention of researchers due to its strong immunogenicity and also because it serves as coadjunct immunomodulator of the immune response of the intestinal mucosae for the mixed added antigens as well as for those covalently linked to the toxin. the immunopathogeny of cholera is a complex phenomenon. this article presents the preliminary results of experiments conducted with laboratory rats in order to find the iga intestinal response of rodents and humans. | 1994 | 9377108 |
| molecular characterization of a human group a rotavirus isolated from an adult with severe dehydrating diarrhea and its relationship to strains concurrently circulating among children. | while group a rotavirus is widely accepted as an important etiology of acute gastroenteritis in children, this agent rarely causes severe diarrhea in adults and, thus, is not considered by physicians to be an etiological agent for such diseases. | 1994 | 15566782 |
| mechanism of quenching of fluorescein by anti-fluorescein igg antibodies. | 1994 | 303233 | |
| [dementia disorders among older people; description of disease conditions and odontologic consequences]. | 1994 | 2142599 | |
| [carcinoma of the male breast; considerations based on 5 cases]. | 1994 | 302958 | |
| [neurologic manifestations of classical collagenoses]. | 1994 | 205497 | |
| legal abortion. | 1994 | 303225 | |
| simple sensitive technique for detecting organochlorine pesticides on thin layer chromatograms. | chlorinated hydrocarbon pesticides can be quickly detected using commercially available thin layer chromatographic plates dipped in an acetone solution of silver nitrate. the limits of detection are functions of the pesticide, adsorbent, developing system, and concentration of the silver nitrate in acetone solution. on exposure to ultraviolet light, 0.002 microgram 2,4,5-t produced clear darkening within 30 min on precoated silica gel plates (polyvinyl alcohol binder) coated with a solution of 0 ... | 1994 | 197057 |
| posterior inferior cerebellar artery (pica) aneurysm presenting with sah and contralateral crural monoparesis: a case report. | a patient with contralateral monoparesis of the leg due to subarachnoid hemorrhage (sah) from an aneurysm of the first posterior inferior cerebellar artery (pica) segment is reported. the monoparesis may well be associated with the close anatomical relationships between the site of the aneurysm and the pica blood supply of the corticospinal fibers to the contralateral leg. | 1994 | 1615373 |
| [adenoma of the duodenal glands as a source of intestinal hemorrhaging]. | 1994 | 303311 | |
| nucleotide sequence of trnaphe from the seeds of lupin (lupinus luteus). comparison of the major species with wheat germ trnaphe. | the nucleotide sequence of trnaphe of yellow lupin seeds (lupinus luteus) is deduced from the composition of pancreatic and t1 ribonuclease digestion products and compared with trnaphe of wheat germ. major lupin trnaphe, unlike pea trnaphe, differs from wheat germ trnaphe in the first base pair of stem tpsic ("e"). | 1994 | 264239 |
| education and professional training of biomedical engineers in west germany. | the health service and educational systems, together with industry and research centres, are the primary institutions involved in the development of the bio-engineering profession in west germany. since industry and research institutions in west germany are similar in structure to those in other countries, for the purposes of international comparison only the health service and educational systems are described. the questions "how many bioengineers are needed?" and "how many should be trained?", ... | 1994 | 264210 |
| the pna--a potent force in public health. | 1994 | 45236 | |
| drug and non-drug factors influencing adverse reaction to pyrazoles. | some of the factors influencing the likelihood of the appearance of adverse drug reactions in patients are identified and discussed. ways of quantifying some of the risks of adverse reactions in different patients are demonstrated. it is pointed out that adverse reaction data can give valuable guidance for both day-to-day patient management and for the initiation or guidance of research projects. in this latter connection, this study highlights the difference in time of onset between aplastic an ... | 1994 | 303187 |
| new introducer for subcutaneous placement of cerebrospinal fluid shunts: technical note. | a new introducer system for subcutaneous placement of shunt tubings is described. the advantages of this system over previous introducers are discussed. | 1994 | 264158 |
| therapeutic options in meniere's disease. | emphasis on treatment in meniere's disease has recently shifted from a concentration on the vertiginous component alone to include conservation or improvement of hearing as well. this presentation tabulates the current otological literature's reports of both the medical and surgical options available in the treatment of meniere's disease. the authors conclude that these more recent reports deemphasize the importance of the vertiginous component, while emphasizing the importance of the hearing co ... | 1994 | 303093 |
| in vitro studies of frog mucous glands. | the ionic outflow, mainly consisting of na+ and cl-, from the mucous glands in an excised nerve-skin preparation of frog has been determined by recording the conductance changes occurring in a fluid layer covering a small area of the skin surface. in the main series of experiments the glands were activated by stimulation of sympathetic nerve fibers in the skin nerve. the relationship between the ionic outflow and the number of nerve volleys was studied over a wide range. the outflow per impulse ... | 1994 | 303041 |
| elastosis and other stromal reactions in benign and malignant breast tissue: an ultrastructural study. | the stroma and stromal reaction in normal breast, benign, and malignant breast tissue was studied by electron microscopy. elastosis is the main stromal response in infiltrating duct and lobular carcinomas. medullary carcinoma elicits no significant elastosis but intraductal carcinoma has significant local elastosis. benign conditions such as fibroadenoma and sclerosing adenosis produced no significant elastosis. the high number of elastic fibers and the high microfibril:elastin ratio indicate th ... | 1994 | 196733 |
| [discrimination of ischemic- and glycoside-conditioned st-t segment changes in stress ecg]. | 1994 | 1029953 | |
| [spatial deployments of morphogenetic movements as elements of the oral field in anuran amphibians. i. structurally stable morphogenetic movements]. | the movements of cells in the oral field of anuran embryos were followed by means of orto- and heterotopic transplantations of the ectoderm fragments. to mark individual ectoderm regions, the embryos were used which differed from each other by colour. the cells polarize along the apical-basal layer axis and the polarization spreads along the layer as a waver embracing consecutively new and new cells. the waves of morphogenetic rearrangements from the spatial deploiments of morphogenetic movement ... | 1994 | 302926 |
| sexual aspects of the female diabetic. | 1994 | 302897 | |
| psychological needs of the spinal cord injured person. | 1994 | 263762 | |
| letitia a. westgate, m.d. (1866-1941?): feminist, idealist, healer, maverick, and heretic. | 1994 | 201689 | |
| inhibition of chymotrypsin by peptidyl trifluoromethyl ketones: determinants of slow-binding kinetics. | a series of seven peptidyl trifluoromethyl ketone (tfk) inhibitors of chymotrypsin have been prepared which differ at the p1 and p2 subsites. inhibition equilibria and kinetics of association and dissociation with chymotrypsin have been measured. the association rate of ac-phe-cf3 was measured at enzyme concentrations between 8 nm and 117 microm in order to examine the relation between the ketone/hydrate equilibrium of trifluoromethyl ketones and the "slow binding" by these inhibitors. the assoc ... | 1994 | 2271521 |
| college age lesbianism as a developmental phenomenon. | 1994 | 264045 | |
| comparison of [125i]somatomedin a and [125i]somatomedin c radioreceptor assays for somatomedin peptide content in whole and acid-chromatographed plasma. | the placental membrane radioreceptor assay was used to measure the levels of somatomedin (sm) peptides in plasma. displacement of both [125i]somatomedin a ([125i]sm-a) and [125i]somatomedin c ([125i]sm-c) by normal whole plasma, the peptide fraction of acid-chromatographed plasma, and a partially purified, insulin-free sm preparation were compared. the peptide fraction of plasma was isolated by acid chromatography over sephadex g-50 in 0.25 m formic acid with a yield of greater than or equal to ... | 1994 | 263658 |
| unusual form of tuberculosis of lips. | 1994 | 267179 | |
| depression, cancer, and guilt--what is the connection? | 1994 | 263143 | |
| defect in the integrity of an epidural catheter. | 1994 | 2271476 | |
| enzyme-linked immunosorbent assays. | 1994 | 2486571 | |
| rehabilitation medicine in sweden. part 2 - education and research. | 1994 | 263010 | |
| recovery curves: uses and limitations. | 1994 | 263006 | |
| cholera toxin gene polymerase chain reaction for detection of non-culturable vibrio cholerae o1. | cholera enterotoxin is a major antigenic determinant for virulence of vibrio cholerae o1 which can enter into a viable but non-culturable (n-c) state, not detectable by conventional culture methods, yet remain capable of producing enterotoxin and potentially pathogenic. pcr was applied in the current study to detect the chilera toxin (ctx) gene of n-c cells, thus eliminating the necessity of culture. sets of oligonucleotide primers were designed, based on the ctxab operon of v. cholerae o1, to d ... | 1994 | 24421136 |
| antimicrobial susceptibility of non-o1 vibrio cholerae isolated from wastewater stabilization ponds in marrakesh, morocco. | the 365 strains of vibrio cholerae, isolated in marrakesh from raw sewage and stabilization pond effluent, were all identified as non-o1 vibrio cholerae. when tested for their susceptibilities to ampicillin, amoxicillin, cephalothin, streptomycin, novobiocin, chloramphenicol, nalidixic acid and trimethoprim-sulphamethoxazole, 13% of the strains from raw sewage and 20% of those from stabilization pond effluent were found to be resistant to one or more of the antibiotics. there were no significant ... | 1994 | 24420955 |
| production of n-acetylglucosamine deacetylase by vibrio cholerae non-o1. | vibrio cholerae non-o1 (1148 a) produced β-n-acetylglucosamini-dase and n-acetylglucosamine (glcnac) deacetylase intracellularly when grown in chitin or glcnac containing medium. it also secreted chitinase only in the chitin-containing medium. the partially purified glcnac deacetylase deacetylated glcnac but not chitin oligosaccharides, the dimer to hexamer of glcnac. we also detected the reaction product by capillary electrophoresis. | 1994 | 27315721 |
| vibrio mimicus are the reservoirs of the heat-stable enterotoxin gene (nag-st) among species of the genus vibrio. | using a 0.27 kb dna probe specific for the heat-stable enterotoxin gene (nag-st) of vibrio cholerae non-o1, 1109 strains representing 17 species of the genus vibrio, isolated from clinical and environmental sources were examined. the nag-st gene was preponderantly associated with strains classified as v. mimicus; 16.8% of these strains hybridized. it was more frequent in the clinical isolates (22.6%) than in the environmental isolates (13.7%). the incidence of nag-st gene-positive strains of v. ... | 1994 | 24420888 |
| [cytodiagnosis of the radiologically "occult" and the "in situ" lung neoplasm]. | this paper reports on six patients, in whom a so-called "carcinoma in situ" or a radiologically "occult" carcinoma of the lung was diagnosed by means of cytological sputum examinations. on the basis of these cases, the problem of early localization of lung carcinomas is discussed. the cytological diagnosis of an occult lung carcinoma is practically always the result of an incidental sputum examination. nevertheless, the value of "mass screenings" is doubtful, whereas systematic sputum examinatio ... | 1993 | 204560 |
| [diaket as a root canal filling material]. | 1993 | 265789 | |
| [care of the injured in the hospital. medico-legal documents furnished them. transmitting care information]. | 1993 | 262925 | |
| postsynaptic effects of a multiaction giant interneurone on identified snail neurones. | 1993 | 196209 | |
| health effects of the 1991 bangladesh cyclone: report of a unicef evaluation team. | to assess the impact on health of the cyclone and tidal wave that struck the southern coast of bangladesh on the evening of 29 april 1991, a team of health professionals visited cyclone affected areas from 4-27 june, 1991. team members met with health workers and officials of the government of bangladesh and with staff pom nongovernmental organizations, and conducted field surveys in two severely affected areas. mortality among the 135 households surveyed (pre-cyclone population 1,123) was 14 pe ... | 1993 | 20958764 |
| environmental influences on the distribution of the incidence of cholera: a case study in quelimane, mozambique. | cholera continues to cause widespread suffering in many parts of the world. previous research has mainly described modes of transmission and has correctly indicated the role of predisposing socio-economic conditions in affected areas. little field research has been carried out, however, in endemic zones, on the contribution of physical characteristics in environmental reservoirs which prolong the survival time or increase the toxigeneity of vibrio cholerae 01, despite substantial indication of t ... | 1993 | 20958774 |
| vibrio cholerae non-0:1 meningitis in an infant. | 1993 | 8284127 | |
| epidemic diarrhoea due to vibrio cholerae non-o1. | 1993 | 8695332 | |
| [cholera in ukraine and moldova during the 7th pandemic]. | the epidemic manifestations of cholera in the ukraine and moldova in 1970-1991 are analyzed. in the ukraine the peak of cholera morbidity in 1970 was caused by infection brought to the ports of odessa and kerch with its subsequent spread to other territories. this infection was characterized by outbreaks transmitted through water, mainly sea water, with the isolation of vibrio eltor toxigenic virulent strains and outbreaks of vibrio carriership of alimentary (dairy) origin with the isolation of ... | 1993 | 8059573 |
| [ultrastructural bases of microcirculatory disorders in intraganglionic capillaries and disorders of nerve elements in the small intestine of suckling rabbits in experimental cholera]. | the 10-12-day suckling rabbits received culture of v. el tor into the stomach, and the ultrastructural changes of intraganglionic capillaries and nervous elements of small intestine were studied in the course of experimental cholera. it was established that the initial changes 1.5 hrs after the beginning of the experiment were characterized by permeability increase, thrombohemorrhagic syndrome development, oedema of endotheliocytes and perivascular space, fibrin precipitation. there were reactiv ... | 1993 | 7980036 |
| the omps gene of vibrio cholerae encodes a growth-phase-dependent maltoporin. | the outer membrane of vibrio cholerae contains a maltose-inducible major protein, omps (43 kda), that is common to different isolates. nucleotide sequence analysis of the corresponding structural gene, omps, revealed an open reading frame encoding a 412-amino-acid polypeptide. the amino acid sequence of omps is similar to that of lamb, the escherichia coli maltoporin, and to scry or klebsiella pneumoniae, although the antigenic determinants of these proteins are different. the cloned omps gene c ... | 1993 | 7934851 |
| algal blooms in the spread and persistence of cholera. | cholera has been long associated with the seasonality of coastal algal blooms off bangladesh. using fluorescent antibody (fa) techniques, microbiologists have now identified a viable, non-cultivable form of vibrio cholerae in a wide range of marine life, including cyanobacteria (anabaena variabilis), diatoms (skeletonema costatum), phaeophytes (ascophyllum nodosum), in copepod molts, and in freshwater vascular aquatic plants (water hyacinths and duckweed). in unfavourable conditions v. cholerae ... | 1993 | 8155853 |
| [cholera immunology and the molecular biology of cholera toxin. recent progress and future prospects]. | cholera toxin (ct) and the analogous heat-labile enterotoxin (lt) from escherichia coli have several immunomodulating effects that might explain their adjuvant action in stimulating secretory mucosal iga after oral immunization. in mice experimental model, these effects include: enhanced antigen presentation by macrophages and other cell types; promotion of isotype differentiation in b cells leading to increased iga formation; and other important effects on t cell proliferation and lymphokine pr ... | 1993 | 8143024 |
| characterization of vibrio cholerae o139 synonym bengal isolated from patients with cholera-like disease in bangladesh. | vibrio cholerae o139 (synonym bengal), a novel serovar of v. cholerae, is the causative agent of large outbreaks of cholera-like illness currently sweeping india and bangladesh. eight randomly selected v. cholerae o139 isolates were studied for their biological properties, which were compared with those of v. cholerae o1 and other v. cholerae non-o1. the v. cholerae o139 isolates were characterized by the production of large amount of cholera toxin, hemagglutination, weak hemolytic properties, r ... | 1993 | 8133802 |
| detection and identification of toxigenic vibrio cholerae o1 strains by a simplified polymerase chain reaction method. | the polymerase chain reaction (pcr) with three oligonucleotide primers was used to amplify both 184 bp and 425 bp dna fragments of cholera toxin a subunit gene (ctxa) for detection and identification of toxigenic v. cholerae o1 strains in one step. for enhancing the sensitivity, we have used the chemiluminescent detection system for southern hybridization. a total of 92 vibrio strains were tested by both pcr and enzyme-linked immunosorbent assay (elisa). three e. coli (etec) strains, one toxigen ... | 1993 | 8131662 |
| [a study of the biological properties of cholera phages and strains of el-tor vibrions isolated from the external environment in donetsk]. | choleric phages were identified in 2 out of 44 samples of donetsk sewage. biological properties of phages were characterized. | 1993 | 8125357 |
| [bacteriological aspects of cholera in benin (epidemic of 1991)]. | this study showed that the strains of vibrio cholerae el tor, ogawa serotype, isolated during the last cholera outbreak in benin (1991) are widely sensitive to tetracyclin (84%), sulfamid (96%), ampicillin (98%). | 1993 | 8124099 |
| vibrios among patients of good socioeconomic conditions during the cholera epidemic in recife, brazil. | between march and july, 1992, we screened for vibrio all fecal samples submitted for bacteriologic diagnosis at a private clinical laboratory in recife. of 1435 cultures examined only 1 (0.07%) was positive for v. cholerae 01, biovar eltor, serovar inaba, but 17 (1.2%) yielded non-cholera vibrio (v. cholerae non-01; v. fluvialis; v. furnissii, v. parahaemolyticus and vibrio spp). thus, v. cholerae 01, differently of other enteropathogenic vibrios, spared individuals of good socioeconomic conditi ... | 1993 | 8115794 |
| in vitro and in situ survivals of bacterial populations added to fresh water environments. | the fate of aeromonas hydrophila, alcaligenes denitrificans, vibrio cholerae non-01, pseudomonas putida and four different isolates of escherichia coli in fresh river water were assessed by using different microcosms (i.e., membrane diffusion chamber and erlenmeyer flask). when water samples were incubated at 16 +/- 1 degrees c, the differences in extent of survival among test bacteria were in general not significant. if the incubation temperature was raised to 29 +/- 1 degrees c, in the in situ ... | 1993 | 7982366 |
| [the determination of the toxigenicity of vibrio cholerae strains by using a culture of chinese hamster ovary cells]. | 1993 | 8079536 | |
| localization of vibrio cholerae o1 in the intestinal tissue. | colonization of v. cholerae o1 in vivo is known to be a non-invasive type which the vibrios are confined only to the intestinal tissues. the pathway by which the vibrio antigens reach the lymphoid cells and subsequently give rise to the immune responses is not entirely clear. thus, experiments were performed in experimental rats by inoculating live v. cholerae o1 into the ligated ileal loops. the fate of the vibrios in the intestinal tissues was then studied by transmission electron microscopy a ... | 1993 | 8080608 |
| problems and prospects of a traditional source of potable water in hilly terrains. | during epidemic investigation, water sources were tested for quality for drinking purpose. out of 30 khatris tested, water of 86.7% khatris was fit for human consumption and fecal coliform was not found in any of them. modification of these khatris was stressed by respondents. | 1993 | 8076998 |
| occurrence of vibrio cholerae 01 strains in southeast asia resistant to vibriostatic compound 0/129. | 1993 | 7939959 | |
| in vitro production of toxin-coregulated pili by vibrio cholerae el tor. | toxin-coregulated pili (tcp) have been shown to be a virulence determinant and protective antigen for vibrio cholerae strains of classical biotype, but their role in infection by strains of the alternative el tor biotype remains uncertain. in an attempt to demonstrate tcp production by el tor vibrios we have over-expressed the el tor tcpa gene in escherichia coli, in order to prepare a biotype-specific anti-tcpa serum. this reagent proved to be a very sensitive indicator of tcpa production in im ... | 1993 | 7906005 |
| summary of the 27th united states-japan joint conference on cholera and related diarrheal diseases. | 1993 | 8093361 | |
| biophysical characterization of vibrio el tor typing phage e5. | vibrio cholerae typing phage e5, which can lyse only the el tor strains of v. cholerae, was characterized. the phage had a polyhedral head 51 nm in diameter and a short tail 13 nm in length. it contained 13 structural polypeptides, with the molecular mass of the major component being 50 kda. phage chromosome comprised a 38.5-kb linear double-stranded dna molecule with unique termini, as determined by restriction fragment analysis and electron microscopy, and had a g+c content of 35.5%. a physica ... | 1993 | 8095039 |
| emergence of novel strain of vibrio cholerae with epidemic potential in southern and eastern india. | 1993 | 8095620 | |
| large outbreak of clinical cholera due to vibrio cholerae non-o1 in bangladesh. | 1993 | 8095621 | |
| polymyxin b sensitive strains of vibrio cholerae non-o1 from recent epidemic in india. | 1993 | 8096977 |