Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
effectiveness of three age estimation methods based on dental and skeletal development in a sample of young brazilians.this study compared three methods designed for age estimation.201829100105
mineral fortification modifies physical and microstructural characteristics of milk gels coagulated by a bacterial enzymatic pool.an enzymatic pool from the amazonian bacterium bacillus sp. p7 was used as milk coagulant. discovery of novel coagulants is of great interest in dairy industry for the development of new textures in cheese. color, mechanical and microstructural characterization of milk gels induced by the bacterial enzymatic pool was carried out. effect of mineral fortification on these characteristics was studied. whiter gels with smaller pore diameters were obtained in the presence of ca2+ or mg2+. these chara ...201829096374
concordance between intention to use and current use of contraceptives among six-month postpartum women in brazil: the role of unplanned pregnancy.to examine the effect of pregnancy planning status in the concordance between intention to use and current use of contraceptives among postpartum women.201829096285
combined effect of copper sulfate and water temperature on key freshwater trophic levels - approaching potential climatic change scenarios.this work relied on the use microcosms to evaluate the individual and the combined effects of different levels of copper sulfate (0.0, 0.013, 0.064 and 0.318mg cu l-1) - a fungicide commonly exceeding allowable thresholds in agricultural areas - and a range of water temperature increase scenarios (15, 20 and 25°c) on freshwater species belonging to different functional groups. hence, the growth inhibition of primary producers (the microalgae raphidocelis subcapitata and the macrophyte lemna mino ...201829096265
mixtures of diflubenzuron and p-chloroaniline changes the activities of enzymes biomarkers on tilapia fish (oreochromis niloticus) in the presence and absence of soil.the insecticide diflubenzuron (dfb), used by many fish farming, when metabolized or degraded produces the extremely toxic compound p-chloroaniline (pca). once in the aquatic environment, these compounds can form mixtures and their bioavailability depends on factors such as the presence of soil. the toxic effects of the isolated compounds and their mixtures in the proportions: 75%, 50%, and 25% of pca were analyzed in tilapia (oreochromis niloticus) in the presence and absence of soil after 96h. ...201829096263
bioaugmentation of strain methylobacterium sp. c1 towards p-nitrophenol removal with broad spectrum coaggregating bacteria in sequencing batch biofilm reactors.this work was conducted in order to evaluate an instance of bioaugmentation, namely, the addition of a novel p-nitrophenol (pnp)-degrading bacterium methylobacterium sp. c1 coaggregated with two other broad-spectrum coaggregating strains (bacillus megaterium t1 and bacillus cereus g5) within sequence batch biofilm reactors (sbbrs). results showed that biofilms consisting of c1 and coaggregating bacteria were resistant to shock loads and were more efficient at pnp removal. high-throughput sequenc ...201829096256
eus-guided choledochoduodenostomy versus hepaticogastrostomy: a systematic review and meta-analysis.endoscopic ultrasound-guided biliary drainage (eus-bd) has emerged as an alternative in cases of endoscopic retrograde cholangiopancreatography (ercp) failure. two types of eus-bd methods for achieving biliary drainage when ercp fails are choledochoduodenostomy (cds) or hepaticogastrostomy (hgs). however, there is no consensus if one approach is better than the other. therefore, we conducted a systematic review and meta-analysis to evaluate these 2 main eus-bd methods.201829095426
potential ecotoxicity of metals leached from antifouling paint particles under different salinities.antifouling paint particles (apps) are residues generated during maintenance of vessels. in boat maintenance areas of south america, waste generation and disposal are not completely regulated. therefore, apps can enter into the aquatic environment and act as a source of contamination by metals and other biocides. thus, the aim of the present study was to evaluate the potential ecotoxicity of the metal mixture present in apps leached under different salinities. therefore, the copepod acartia tons ...201829102905
avoidant personality disorder and social phobia: does mindreading make the difference?avoidant personality disorder (avpd) is closely related to and partially overlaps with social phobia (sp). there is an ongoing debate as to whether avpd and sp can be classified as separate and distinct disorders or whether these diagnoses rather reflect different degrees of severity of social anxiety. the hypothesis of this study is that in patients with avpd and in those with avpd and comorbid sp both interpersonal functioning and metacognitive abilities (the ability to understand mental state ...201829096207
sulfamate esters guide selective radical-mediated chlorination of aliphatic c-h bonds.masked alcohols are particularly appealing as directing groups because of the ubiquity of hydroxy groups in organic small molecules. herein, we disclose a general strategy for aliphatic γ-c(sp3 )-h functionalization guided by a masked alcohol. specifically, we determine that sulfamate ester derived nitrogen-centered radicals mediate 1,6-hydrogen-atom transfer (hat) processes to guide γ-c(sp3 )-h chlorination. this reaction proceeds through a light-initiated radical chain-propagation process and ...201829096044
effectiveness and toxicity of first-line methotrexate chemotherapy in low-risk postmolar gestational trophoblastic neoplasia: the new england trophoblastic disease center experience.to assess the outcomes and toxicity of first-line methotrexate (mtx) chemotherapy in low-risk postmolar gestational trophoblastic neoplasia (gtn) patients receiving 8-day methotrexate or one-day methotrexate infusion regimens.201829092742
engineering an e. coli near-infrared light sensor.optogenetics is a technology wherein researchers combine light and genetically engineered photoreceptors to control biological processes with unrivaled precision. near-infrared (nir) wavelengths (>700 nm) are desirable optogenetic inputs due to their low phototoxicity and spectral isolation from most photoproteins. the bacteriophytochrome photoreceptor 1 (bphp1), found in several purple photosynthetic bacteria, senses nir light and activates transcription of photosystem promoters by binding to a ...201829091422
photobiomodulation therapy for the improvement of muscular performance and reduction of muscular fatigue associated with exercise in healthy people: a systematic review and meta-analysis.researches have been performed to investigate the effects of phototherapy on improving performance and reduction of muscular fatigue. however, a great variability in the light parameters and protocols of the trials are a concern to establish the efficacy of this therapy to be used in sports or clinic. the aim of this study is to investigate the effectiveness, moment of application of phototherapy within an exercise protocol, and which are the parameters optimally effective for the improvement of ...201829090398
impact of water level fluctuations on the development of phytoplankton in a large subtropical reservoir: implications for the management of cyanobacteria.lake water level fluctuations (wlf) are an important factor driving the selection and seasonal dynamics of phytoplankton and potentially toxigenic cyanobacteria. nevertheless, the relative importance of environmental drivers connected to wlf may be completely different, depending on the typology and use of waterbodies, latitude and climatic regimes. in this study, we investigated the impact of wlf in a large subtropical reservoir in south-eastern china (hongfeng reservoir, guizhou province). the ...201829086174
effects of edta gel and chlorhexidine gel on root dentin permeability.the aim of this study was to evaluate the effect of 24% ethylenediaminetetraacetic acid (edta) gel and 2% chlorhexidine gel (chx) in dentin permeability and smear layer removal from root canals instrumented with niti rotary system using histochemical staining and scanning electron microscopy (sem) analysis. overall, 43 premolars were classified into two experimental groups, edta (n = 20) and chx (n = 20), and a negative control (nc) (n = 3). all specimens were instrumented and the irrigant solut ...201829083081
author response, "the water-drinking test and glaucoma progression: considerations regarding the test usefulness as an independent risk assessment tool". 201829088050
adaptation in toxic environments: comparative genomics of loci carrying antibiotic resistance genes derived from acid mine drainage waters.several studies have suggested the existence of a close relationship between antibiotic-resistant phenotypes and resistance to other toxic compounds such as heavy metals, which involve co-resistance or cross-resistance mechanisms. a metagenomic library was previously constructed in escherichia coli with dna extracted from the bacterial community inhabiting an acid mine drainage (amd) site highly contaminated with heavy metals. here, we conducted a search for genes involved in antibiotic resistan ...201829090447
spatio-temporal gene induction systems in the heterocyst-forming multicellular cyanobacterium anabaena sp. pcc 7120.in the last decade, much progress has been made in the photosynthetic production of valuable products using unicellular cyanobacteria. however, production of some products requires dark, anaerobic incubation, which prevents practical applications using these organisms. anabaena sp. pcc 7120 (a. 7120) is a heterocyst-forming multicellular cyanobacterium that is easy to manipulate genetically. upon nitrogen step-down, this strain differentiates heterocysts that retain micro-oxic conditions for nit ...201829088489
evaluation of genotoxic effects in brazilian agricultural workers exposed to pesticides and cigarette smoke using machine-learning algorithms.monitoring exposure to xenobiotics by biomarker analyses, such as a micronucleus assay, is extremely important for the precocious detection and prevention of diseases, such as oral cancer. the aim of this study was to evaluate genotoxic effects in rural workers who were exposed to cigarette smoke and/or pesticides and to identify possible classification patterns in the exposure groups. the sample included 120 participants of both sexes aged between 18 and 39, who were divided into the following ...201829086360
library growth and protein expression: optimal and reproducible microtiter plate expression of recombinant enzymes in e. coli using mtp shakers.escherichia coli (e. coli) as heterologous host enables the recombinant expression of the desired protein in high amounts. nevertheless, the expression in such a host, especially by utilizing a strong induction system, can result in insoluble and/or inactive protein fractions (inclusion bodies). furthermore, the expression of different enzyme variants often leads to a diverse growth behavior of the e. coli clones resulting in the identification of false-positives when screening a mutant library. ...201829086307
comparative genomics reveals cotton-specific virulence factors in flexible genomic regions in verticillium dahliae and evidence of horizontal gene transfer from fusarium.verticillium dahliae isolates are most virulent on the host from which they were originally isolated. mechanisms underlying these dominant host adaptations are currently unknown. we sequenced the genome of v. dahliae vd991, which is highly virulent on its original host, cotton, and performed comparisons with the reference genomes of jr2 (from tomato) and vdls.17 (from lettuce). pathogenicity-related factor prediction, orthology and multigene family classification, transcriptome analyses, phyloge ...201829084346
marked differences in the submandibular salivary proteome between sardinian alcohol-preferring and sardinian alcohol-non preferring rats revealed by an integrated top-down-bottom-up proteomic platform.sardinian alcohol-preferring (sp) and sardinian alcohol-non preferring (snp) rats have been selectively bred for opposite alcohol preference and consumption. aiming to verify possible differences at the proteomics level between sp and snp rats, we investigated the salivary proteome by a a liquid chromatography-mass spectrometry top-down-bottom-up integrated approach. for this purpose, submandibular saliva was collected from alcohol-naive sp and snp rats under isoprenaline stimulation. a total of ...201829083190
virulence differences in blumeria graminis f. sp. tritici from the central and eastern united states.wheat powdery mildew is a disease of global importance that occurs across a wide geographic area in the united states. a virulence survey of blumeria graminis f. sp. tritici, the causal agent, was conducted by sampling 36 wheat fields in 15 u.s. states in the years 2013 and 2014. using a hierarchical sampling protocol, isolates were derived from three separated plants at each of five separated sites within each field in order to assess the spatial distribution of pathotypes. in total, 1,017 isol ...201829082810
high level of b19 strain detection in brazilian cattle semen.semen contaminated with microorganisms can disseminate serious diseases including brucellosis. the objectives of this study were to detect brucella-specific antibodies and brucella abortus dna in samples of blood and fresh semen from 100 animals older than 20 months. the samples were collected on farms and in semen collection and processing centers (ccps). the serum samples were evaluated by rose bengal test (rbt). b. abortus dna was detected by a polymerase chain reaction (pcr) using bab and is ...201829082458
taurine supplementation can increase lipolysis and affect the contribution of energy systems during front crawl maximal effort.taurine can affect the energy system metabolism, specifically the lipid metabolism, since an increase in lipid oxidation may promote carbohydrate savings. we hypothesized that taurine supplementation associated with high-intensity exercise could increase levels of lipolysis, benefiting swimmer performance. nine male competitive swimmers performed two 400-m front crawl maximal efforts with a 1-week washout, and the athletes received 6 g of taurine (tau) or placebo (pla) supplementation 120 min be ...201829082444
chronic social stress ameliorates psoriasiform dermatitis through upregulation of the hypothalamic-pituitary-adrenal axis.acute stress is a physiological response of an organism to adverse conditions, contributing to survival; however, persistence through time may lead to disease. indeed, exacerbation of inflammatory conditions such as psoriasis has been reported to follow stressors in susceptible patients. because chronic stress cannot ethically be elicited in patients under controlled laboratory conditions, we studied genetically modified mice that naturally develop psoriasiform dermatitis, and subjected them to ...201829080684
amygdaloid involvement in the defensive behavior of mice exposed to the open elevated plus-maze.previous studies have shown that the exposure to an open elevated plus maze (oepm, an epm with all four open arms) elicits fear/anxiety-related responses in laboratory rodents. however, very little is known about the underlying neural substrates of these defensive behaviors. accordingly, the present study investigated the effects of chemical inactivation of the amygdala [through local injection of cobalt chloride (cocl2: a nonspecific synaptic blocker)] on the behavior of oepm-exposed mice. in a ...201829080676
visible and near infrared spectroscopy coupled to random forest to quantify some soil quality parameters.this study evaluates the use of visible and near infrared spectroscopy (vis-nirs) combined with multivariate regression based on random forest to quantify some quality soil parameters. the parameters analyzed were soil cation exchange capacity (cec), sum of exchange bases (sb), organic matter (om), clay and sand present in the soils of several regions of brazil. current methods for evaluating these parameters are laborious, timely and require various wet analytical methods that are not adequate ...201829080499
young drivers and their cars: safe and sound or the perfect storm?consistent with the experiences in high-income countries, young drivers remain overrepresented in road trauma statistics in low- and middle-income countries. this article pursues the emerging interest of approaching the young driver problem from a systems thinking perspective in order to design and deliver robust countermeasures. specifically, the focus of this paper is the cars driven by young drivers. the study of vehicles' characteristics and their interaction with driving behaviour is, more ...201829080470
diversity and characteristics of the meat microbiological community on dry aged beef.beef was dry aged for 40-60 days under controlled environmental conditions in a refrigerated room with a relative humidity of 75%-80% and air-flow. to date, there is little information on the microbial diversity and characteristics of dry aged beef. in this study, we explored the effect of change in meat microorganisms on dry aged beef. initially, the total bacteria and lab were significantly increased for 50 days during all dry aging periods. there was an absence of representative foodborne pat ...201829081086
detection of candida species by nested pcr method in one-humped camels (camelus dromedarius).systemic fungal diseases are the infections caused by false treatment protocols and generally are not taken into consideration especially in the veterinary field. one-humped camels are found in the western side of the aegean region of our country and bred for wrestling. the aim of this study is the application of diagnosing systemic fungi infection from camel blood samples by the pcr method. in this study, specific primers for dna topoisomerase ii gene sequences were used. as a result, a systemi ...201829080936
increased cocaine and amphetamine-regulated transcript cord blood levels in the newborns exposed to crack cocaine in utero.cocaine and amphetamine-regulated transcript (cart) is an endogenous antioxidant present since the embryonic period. cart is activated by high levels of dopamine and might be of interested in understanding the changes in the redox system associated with crack/cocaine intake. the goal of this study was to determine whether exposure to crack in utero is associated with increased cart levels.201829080905
evaluation of food intake and fos expression in serotonergic neurons of raphe nuclei after intracerebroventricular injection of adrenaline in free-feeding rats.previous studies indicate that the modification of adrenergic neurotransmission in median raphe nucleus (mrn) enhances or removes an inhibitory influence on food intake, possibly serotonergic, due to a presence of serotonin-producing neurons in that nucleus. therefore, the aim of this study is evaluated whether the activity of neurons in the mrn and dorsal raphe nucleus (drn) are affected by intracerebroventricular injection of adrenaline (ad) in free-feeding rats. male wistar rats with guide ca ...201829079504
is adenosine associated with sudden death in schizophrenia? a new framework linking the adenosine pathway to risk of sudden death.schizophrenia is associated with an increased mortality from cardiovascular disease. relatively few studies have assessed the putative association of schizophrenia pathophysiology with sudden death. low adenosine levels have been associated with schizophrenia. in cardiology, increased mortality among patients with congestive heart failure has been associated with genetic polymorphisms that potentially lead to lower adenosine levels. thus, we hypothesize that adenosine could link schizophrenia an ...201829079493
use of sodium trimetaphosphate in the inhibition of dentin matrix metalloproteinases and as a remineralizing agent.because of its ability to act as an antiproteolytic agent, the effect of sodium trimetaphosphate (stmp) against specific enzymes extracted from sound dentin and its performance under acidic challenge on demineralized dentin were investigated.201829079452
co-production of polyhydroxyalkanoates and carotenoids through bioconversion of glycerol by paracoccus sp. strain ll1.polyhydroxyalkanoates (pha) have emerged as a potential alternative to synthetic plastics, although its large-scale production is limited by the expenditure incurred in feed materials. co-production of various valuable bioproducts along with pha has been proposed to alleviate the overall production cost. here, high-yield co-production of pha and carotenoids was achieved in single step fermentation by paracoccus sp. ll1. the halophilic bacterial strain could metabolize a wide range of substrates ...201829079434
internal fixation of displaced intra-articular fractures of the hallux through a dorsomedial approach: a technical tip.phalangeal fractures of the toes represent common injuries of the forefoot. in the hallux, most fractures occur at the distal phalanx and frequently result from a direct crushing type of injury. intra-articular fractures of the hallux are usually treated nonoperatively, except when the fragments are displaced and the joint is incongruent. displaced fractures treated nonoperatively can result in degenerative arthritis of the interphalangeal joint, causing pain and range of motion limitation, hind ...201829076759
movement system impairment-based classification treatment versus general exercises for chronic low back pain: randomized controlled trial.treatment for chronic low back pain (lbp) includes different forms of exercises, that to date have resulted in only small to moderate treatment effects. to enhance the treatment effects, different classification systems have been developed to classify people with lbp into more homogeneous subgroups leading to specific treatments for each subgroup.201829077963
performance and serum biochemical profile of japanese quail supplemented with silymarin and contaminated with aflatoxin b1.the aim of this study was to evaluate the hepatoprotective effect of silymarin in diets contaminated or not with aflatoxin b1 (afb1) on the productive performance and serum biochemical profile of japanese quail (coturnix coturnix japonica) in the laying phase. a total of 240 12-week-old japanese quail was used in a completely randomized design in a 3 × 2 factorial scheme (additives x contaminated or not with afb1 - 1,500 μg/kg), totaling 6 treatments and 5 replicates of 8 birds each. the additiv ...201829077959
effects of deinococcus spp. supplement on egg quality traits in laying hens.to counter the ill effects of synthetic dyes, bacterial pigment production as an alternative is now one of the promising and emerging fields of research. this study was conducted to evaluate the applicability of deinococcus genus on the egg quality traits in laying hens. in study i, 24 single comb white leghorn layers were fed with various 1 wt % deinococcus bacterial strains for 10 d. in study ii, 84 brown hendrix layers were fed with one of 4 diets containing 0, 0.2, 1, or 5 wt % deinococcus s ...201829077878
compartmentalization of the carbaryl degradation pathway: molecular characterization of inducible periplasmic carbaryl hydrolase from pseudomonas spp.pseudomonas sp. strains c5pp and c7 degrade carbaryl as the sole carbon source. carbaryl hydrolase (ch) catalyzes the hydrolysis of carbaryl to 1-naphthol and methylamine. bioinformatic analysis of mcba, encoding ch, in c5pp predicted it to have a transmembrane domain (tmd) and a signal peptide (sp). in these isolates, the activity of ch was found to be 4- to 6-fold higher in the periplasm than in the cytoplasm. the recombinant ch (rch) showed 4-fold-higher activity in the periplasm of escherich ...201829079626
"there is no carnival without samba": revealing barriers hampering biodiversity-based r&d and eco-design in brazil.considering the unique relevance of brazilian biodiversity, this research aims to investigate the main barriers to biodiversity-based r&d and eco-design development in a leading national company which has been commended for its innovation and sustainability. the methodology for this research was based on on-location visits, in-depth interviews, and consensus building among r&d, sustainability, and quality managers. a multi-criteria decision-making (mcdm) approach was adopted through interpretive ...201829078117
molecular and morphological data reveal non-monophyly and speciation in imperiled freshwater mussels (anodontoides and strophitus).accurate taxonomic placement is vital to conservation efforts considering many intrinsic biological characteristics of understudied species are inferred from closely related taxa. the rayed creekshell, anodontoides radiatus (conrad, 1834), exists in the gulf of mexico drainages from western florida to louisiana and has been petitioned for listing under the endangered species act. we set out to resolve the evolutionary history of a. radiatus, primarily generic placement and species boundaries, us ...201829074460
phenylisotertronic acids from the tcm endophytic fungus phyllosticta sp.four new phenylisotertronic acids (1a/1b, 2a, and 3a) were isolated from a tcm endophytic fungal strain phyllosticta sp. j13-2-12y obtained from the leaves of acorus tatarinowii, along with two known ones (2b and 3b). compounds 1-3 all existed as mixtures of enantiomers, and their corresponding optically pure enantiomers were successfully isolated by chiral hplc. the structures of isolated compounds were determined by comprehensive spectroscopic analyses and x-ray diffraction. their absolute con ...201829074225
phochrodines a-d, first naturally occurring new chromenopyridines from mangrove entophytic fungus phomopsis sp. 33.four new chromenopyridine derivatives, phochrodines a-d (1-4), were identified from mangrove entophytic fungus phomopsis sp. 33# by means of various modern chromatographic, spectroscopic and single crystal x-ray diffraction techniques. compounds 1-4 with an unusual 5h-chromeno[4,3-b]pyridine skeleton were the first naturally occurring chromenopyridines. their anti-inflammatory, antioxidant and cytotoxic activities were evaluated. 3 and 4 showed moderate inhibition of nitric oxide production with ...201829074224
pregnancy diagnosis in cattle using pregnancy associated glycoprotein concentration in circulation at day 24 of gestation.cattle producers are limited to day 28-30 of gestation as the earliest time point for accurate pregnancy diagnosis due to the effectiveness of ultrasound and chemical based methods, including commercially available pregnancy associated glycoproteins (pag) tests. the objective of the current studies were to determine if early gestation circulating pag concentrations at day 24 could be used to diagnose pregnancy in dairy cattle undergoing embryo transfer. in vitro produced embryos were transferred ...201829073542
biodegradation and metabolic pathway of sulfamethoxazole by a novel strain acinetobacter sp.in this study, a novel strain capable of degrading sulfamethoxazole (smx) was isolated and identified as acinetobacter sp. the effect of influencing factors, such as initial smx concentration (5-240 mg/l), temperature (15-35 °c), and ph (5-7), on smx degradation was investigated. the results showed that when the initial smx concentration was in the range of 5-240, the removal efficiency was 100%. the optimal condition for smx biodegradation and microbial growth was determined to be 25 °c and ph  ...201829071423
microbial degradation of fomesafen and detoxification of fomesafen-contaminated soil by the newly isolated strain bacillus sp. fe-1 via a proposed biochemical degradation pathway.fomesafen is a long residual herbicide and poses a potential risk to environmental safety, leading to an increasing need to find eco-friendly and cost-effective techniques to remediate fomesafen-contaminated soils. in this article, a novel strain of bacillus sp., fe-1 was isolated from paddy field soil. this strain was found to degrade fomesafen both in liquid medium and in soil. >82.9% of fomesafen, at concentrations of 0.5, 1 and 10mgl-1, was degraded by bacillus sp. fe-1 in liquid medium with ...201829070446
a rapid and simple hplc method for therapeutic monitoring of vancomycin.therapeutic monitoring of the antibiotic vancomycin is important to achieve specific plasma concentration and prevent toxic effects. several assays have been described for vancomycin determination in clinical practice, but high-performance liquid chromatography is still considered the gold standard for the quantification of vancomycin. in this study, we developed a new and rapid high-performance liquid chromatography method requiring 50 μl of plasma for the quantification of vancomycin. acetonit ...201829069326
latin american scientific contribution to ecology.latin america embodies countries of special interest for ecological studies, given that areas with great value for biodiversity are located within their territories. this highlights the importance of an evaluation of ecological research in the latin america region. we assessed the scientific participation of latin american researchers in ecological journals, patterns of international collaboration, and defined the main characteristics of the articles. although latin american publications have in ...201829069131
mitigating effects of pollen during paraquat exposure on gene expression and pathogen prevalence in apis mellifera l.honey bee (apis mellifera l.) populations have been experiencing notable mortality in europe and north america. no single cause has been identified for these dramatic losses, but rather multiple interacting factors are likely responsible (such as pesticides, malnutrition, habitat loss, and pathogens). paraquat is one of the most widely used non-selective herbicides, especially in developing countries. this herbicide is considered slightly toxic to honey bees, despite being reported as a highly e ...201829067534
microbial community differentiation between active and inactive sulfide chimneys of the kolumbo submarine volcano, hellenic volcanic arc.over the last decades, there has been growing interest about the ecological role of hydrothermal sulfide chimneys, their microbial diversity and associated biotechnological potential. here, we performed dual-index illumina sequencing of bacterial and archaeal communities on active and inactive sulfide chimneys collected from the kolumbo hydrothermal field, situated on a geodynamic convergent setting. a total of 15,701 otus (operational taxonomic units) were assigned to 56 bacterial and 3 archaea ...201829067531
clinical utility of the dsm-5 alternative model for borderline personality disorder: differential diagnostic accuracy of the bfi, scid-ii-pq, and pid-5.with the publication of dsm 5 alternative model for personality disorders it is critical to assess the components of the model against evidence-based models such as the five factor model and the dsm-iv-tr categorical model. this study explored the relative clinical utility of these models in screening for borderline personality disorder (bpd).201829069625
cluster-level relationships of genes involved in carbon metabolism in synechocystis sp. pcc 6803: development of a novel succinate-producing strain.we quantified the transcript levels of 44 genes related to sugar catabolism in strains with altered primary carbon metabolism and discovered a consistent expression pattern among succinate-producing mutants. to identify factors that determine the expression pattern, we calculated pearson's correlation coefficients, using the transcript data. correlation analysis revealed positive and negative correlations among genes encoding sugar catabolic enzymes. on the basis of this analysis, we found that ...201829069477
mutations in algal and cyanobacterial photosystem i that independently affect the yield of initial charge separation in the two electron transfer cofactor branches.in photosystem i, light-induced electron transfer can occur in either of two symmetry-related branches of cofactors, each of which is composed of a pair of chlorophylls (ec2a/ec3a or ec2b/ec3b) and a phylloquinone (phqa or phqb). the axial ligand to the central mg2+ of the ec2a and ec2b chlorophylls is a water molecule that is also h-bonded to a nearby asn residue. here, we investigate the importance of this interaction for charge separation by converting each of the asn residues to a leu in the ...201829066393
'massilibacteroïdes vaginae' gen. nov., sp. nov., a new genus within the family of bacteroïdaceae isolated from a patient with vaginosis.'massilibacteroïdes vaginae' sp. nov. strain mv12t is the type strain of 'massilibacteroïdes' gen. nov., a new genus within the family of porphyromonadaceae. this strain was isolated in marseille from a patient with vaginosis. 'massilibacteroïdes vaginae' is a gram-negative, aero-anaerobic bipolar bacillus. here we describe the phenotypic characteristics and complete genome sequence of this bacterium. the embl accession number is fxdj00000000 (fxdj01000001-fxdj01000009), and its genome consists ...201829071070
increased energy differentially increases richness and abundance of optimal body sizes in deep-sea wood falls.theoretical and empirical studies suggest that the total energy available in natural communities influences body size as well as patterns of abundance and diversity. but the precise mechanisms underlying these relationships or how these three ecological properties relate remain elusive. we identify five hypotheses relating energy availability, body size distributions, abundance, and species richness within communities, and we use experimental deep-sea wood fall communities to test their predicte ...201829065227
accuracy of the pacemaker event recorder versus holter-ecg to detect both symptomatic and asymptomatic ventricular arrhythmias.although new pacemakers can register cardiac rhythm, few studies were performed evaluating their accuracy in diagnosing ventricular arrhythmias (va). this study aimed to assess the correlation and agreement between the pacemaker's monitor and the ambulatory holter in detecting va.201829064614
effect of culture density on biomass production and light utilization efficiency of synechocystis sp. pcc 6803.the viability of large-scale microalgae cultivation depends on providing optimal growth conditions, for which a key operational parameter is culture density. using synechocystis sp. pcc 6803, we conducted a series of fixed-density, steady-state experiments and one batch-growth experiment to investigate the role of culture density on biomass production and light utilization efficiency. in all cases, the fixed-density, steady-state experiments and batch-growth experiment showed good agreement. the ...201829064565
role of beta-adrenergic receptors and sirtuin signaling in the heart during aging, heart failure, and adaptation to stress.in the heart, catecholamine effects occur by activation of beta-adrenergic receptors (β-ars), mainly the beta 1 (β1-ar) and beta 2 (β2-ar) subtypes, both of which couple to the gs protein that activates the adenylyl cyclase signaling pathway. the β2-ars can also couple to the gi protein that counterbalances the effect of the gs protein on cyclic adenosine monophosphate production and activates the phosphatidylinositol 3-kinase (pi3k)-akt signaling pathway. in several cardiovascular disorders, in ...201829063982
bacterial sbp56 identified as a cu-dependent methanethiol oxidase widely distributed in the biosphere.oxidation of methanethiol (mt) is a significant step in the sulfur cycle. mt is an intermediate of metabolism of globally significant organosulfur compounds including dimethylsulfoniopropionate (dmsp) and dimethylsulfide (dms), which have key roles in marine carbon and sulfur cycling. in aerobic bacteria, mt is degraded by a mt oxidase (mto). the enzymatic and genetic basis of mt oxidation have remained poorly characterized. here, we identify for the first time the mto enzyme and its encoding ge ...201829064480
combined effects of binary antibiotic mixture on growth, microcystin production, and extracellular release of microcystis aeruginosa: application of response surface methodology.the interactive effects of binary antibiotic mixtures of spiramycin (sp) and ampicillin (amp) on microcystis aeruginosa (ma) in terms of growth as well as microcystin production and extracellular release were investigated through the response surface methodology (rsm). sp with higher 50 and 5% effective concentrations in ma growth was more toxic to ma than amp. rsm model for toxic unit approach suggested that the combined toxicity of sp and amp varied from synergism to antagonism with sp/amp mix ...201829063395
development of an integrated method of concentration and immunodetection of bacteria.the microbial quality of water is a key aspect to avoid environmental and public health problems. the low pathogen concentration needed to produce a disease outbreak makes it essential to process large water volumes and use sensitive and specific methods such as immunoassays for its detection. in the present work, we describe the development of a device based on microfiltration membranes to integrate the concentration and the immunodetection of waterborne bacteria. a microfiltration membrane tre ...201829063163
biodegradation of sulfamethoxazole in bacteria from three different origins.sulfamethoxazole (smx) is a common medicine prescribed to treat infections. due to vast use, smx has been detected in different parts of the world. hence, it has become a high risk because of its long term persistence with high biological activity in the ecosystem. therefore, it is necessary to understand the mechanism of smx degradation in different genus of bacteria, which is presently unclear. in the present study, degradation of 5 mg l-1 smx was studied in three isolated pure bacterial cultu ...201829059576
analysis and modelling of surface urban heat island in 20 canadian cities under climate and land-cover change.surface urban heat island (suhi) is an urban climate phenomenon that is expected to respond to future climate and land-use land-cover change. it is important to further our understanding of physical mechanisms that govern suhi phenomenon to enhance our ability to model future suhi characteristics under changing geophysical conditions. in this study, suhi phenomenon is quantified and modelled at 20 cities distributed across canada. by analyzing moderate resolution imaging spectroradiometer (modis ...201829059569
guaianolide sesquiterpene lactones and aporphine alkaloids from the stem bark of guatteria friesiana.three guaianolide sesquiterpenes, denoted guatterfriesols a-c, and four aporphine alkaloid derivatives were isolated from the stem bark of the amazonian plant guatteria friesiana. thus far, sesquiterpene lactones have not been described in annonaceae. structures of the previously undescribed compounds were established by using 1d and 2d nmr spectroscopy in combination with ms. the absolute stereochemistry was assigned via noe nmr experiments, ecd spectroscopy, and theoretical calculations using ...201829059536
erding formula in hyperuricaemia treatment: unfolding traditional chinese herbal compatibility using modern pharmaceutical approaches.traditional chinese herbal formulas are difficult to be understood because of complex compositions and specific therapeutic principles. to better understand herbal compatibility in traditional chinese medicine (tcm), this study was conducted to investigate the effects of a chinese pharmacopoeia-listed formula, erding formula (ef) and its constituent herbs for a new indication, hyperuricaemia.201829057475
does mch play a role on establishment or maintenance of social hierarchy in nile tilapia?body coloration has a fundamental role in animal communication by signaling sex, age, reproductive behavior, aggression, etc. nile-tilapia exhibits dominance hierarchy and the dominants are paler than subordinates. during social interactions in these animals, these color changes occur rapidly, and normally the subordinates become dark. in teleosteans, from the great number of hormones and neurotransmitters involved in color changes, melanocyte hormone stimulates (α-msh) and melanin concentrates ...201829056352
the geographic distribution of argas (persicargas) miniatus and argas (persicargas) persicus (acari: argasidae) in america, with morphological and molecular diagnoses from brazil, chile and cuba.high similarity of morphological traits has historically overshadowed the identities and distributions of poultry-associated soft ticks argas (persicargas) miniatus and argas (persicargas) persicus in america. in order to model the occurrence of both parasites in the continent, in the current study we performed morphological and molecular analyses to identify ticks collected in hen houses from brazil and northern chile. combining these results with literature data, and the examination of argas a ...201829055643
molecular detection and characterization of zoonotic anaplasma species in domestic dogs in lusaka, zambia.although tick-borne pathogens, anaplasma platys and anaplasma phagocytophilum are recognized as zoonotic agents associated with appreciable morbidity and mortality in dogs and humans worldwide, there is limited information on these infections in many african countries, including zambia. the purpose of this study was to detect, identify and phylogenetically characterize anaplasma species from dogs in chilanga district in lusaka province, zambia. a total of 301 blood samples were collected from ap ...201829055641
effects of simulated nitrogen deposition on ectomycorrhizae community structure in hybrid larch and its parents grown in volcanic ash soil: the role of phosphorous.with the rapid industrial development and modern agricultural practices, increasing nitrogen (n) deposition can cause nutrient imbalance in immature volcanic ash soil commonly found in japan. larch species, widely distributed in northeast eurasia, are associated with ectomycorrhizal (ecm) fungi which play a critical role in nutrient acquisition for their hosts. in this study, we investigated species richness and diversity of ecm fungi associated with a hybrid larch (f1) and its parents, dahurian ...201829055594
mesenchymal stromal cells modulate gut inflammation in experimental colitis.inflammatory bowel diseases (ibds) affect millions of people worldwide and their frequencies in developed countries have increased since the twentieth century. in this context, there is an intensive search for therapies that modulate inflammation and provide tissue regeneration in ibds. recently, the immunomodulatory activity of adipose tissue-derived mesenchymal stromal cells (admscs) has been demonstrated to play an important role on several immune cells in different conditions of inflammatory ...201829063489
n-acetylborrelidin b: a new bioactive metabolite from streptomyces mutabilis sp. mii.in the course of our screening program for new bioactive compounds, a naturally new 18-membered macrolide antibiotic, n-acetylborrelidin b (1) along with borrelidin (2) were obtained from the marine streptomyces mutabilis sp. mii. the strain was isolated from a sediment sample collected in the red sea at the hurghada coast and characterized taxonomically. additional nine diverse bioactive compounds were reported: 6-prenyl-indole-3-acetonitrile (3), sitosteryl-3β-d-glucoside, campesterol, ferulic ...201829055178
a novel steroid-coenzyme a ligase from novosphingobium sp. strain chol11 is essential for an alternative degradation pathway for bile salts.bile salts such as cholate are steroid compounds with a c5 carboxylic side chain and occur ubiquitously in vertebrates. upon their excretion into soils and waters, bile salts can serve as growth substrates for diverse bacteria. novosphingobium sp. strain chol11 degrades 7-hydroxy bile salts via 3-keto-7-deoxy-δ4,6 metabolites by the dehydration of the 7-hydroxyl group catalyzed by the 7α-hydroxysteroid dehydratase hsh2. this reaction has not been observed in the well-studied 9-10-seco degradatio ...201829054875
bacterial communities in soil become sensitive to drought under intensive grazing.increasing climatic and anthropogenic pressures on soil ecosystems are expected to create a global patchwork of disturbance scenarios. some regions will be strongly impacted by climate change, others by agricultural intensification, and others by both. soil microbial communities are integral components of terrestrial ecosystems, but their responses to multiple perturbations are poorly understood. here, we exposed soils from sustainably- or intensively-managed grasslands in an agro-silvo-pastoral ...201829054674
impact of copper sulfate application at an urban brazilian reservoir: a geostatistical and ecotoxicological approach.a landscape ecotoxicology approach was used to assess the spatial distribution of copper in the recent bottom sediment (surficial sediment) of a brazilian subtropical reservoir (the guarapiranga reservoir) and its potential ecotoxicological impacts on the reservoir ecosystem and the local society. we discuss the policies and procedures that have been employed for the management of this reservoir over the past four decades. spatial heterogeneity in the reservoir was evaluated by means of sampling ...201829054669
influence of organic matter, nutrients, and cyclodextrin on microbial and chemical herbicide and degradate dissipation in subsurface sediment slurries.pesticides leaching from soil to surface and groundwater are a global threat for drinking water safety, as no cleaning methods occur for groundwater environment. we examined whether peat, compost-peat-sand (cps) mixture, nh4no3, nh4no3 with sodium citrate (na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (bam) dissipations in sediment slurries under aerobic and anaerobic conditions, with s ...201829054658
pulsed light reduces the toxicity of the algal toxin okadaic acid to freshwater crustacean daphnia pulex.this constitutes the first study to report on the reduction in toxicity of the dinoflagellate algal toxin okadaic acid after novel pulsed light (pl) treatments where ecotoxicological assessment was performed using a miniaturised format of the conventional in vivo freshwater crustacean daphnia sp. acute toxicity test. bivalves accumulate this toxin, which can then enter the human food chain causing deleterious health effects such as diarrhetic shellfish poisoning. this miniaturised toxicological ...201829052147
optimization of hexanoic acid production in recombinant escherichia coli by precise flux rebalancing.the aim of this study is to demonstrate that rebalancing of metabolic fluxes at acetyl-coa branch node can substantially improve the titer and productivity of hexanoic acid in recombinant escherichia coli strains. first, a hexanoic acid-producing e. coli strain was constructed by expressing genes encoding β-ketothiolase (bktb) from cupriavidus necator and acetyl-coa transferase (act) from megasphaera sp. mh in a butyric acid producer strain. next, metabolic flux was optimized at the acetyl-coa b ...201829054557
networks of phobic fear: functional connectivity shifts in two subtypes of specific phobia.anxiety disorders can be conceptualized by an abnormal interplay of emotion-processing brain circuits; however, knowledge of brain connectivity measures in specific phobia is still limited. to explore functional interactions within selected fear-circuitry structures (anterior cingulate cortex (acc), amygdala, insula), we re-examined three task-based fmri studies using a symptom provocation approach (n=94 subjects in total) on two different phobia subtypes (animal subtype as represented by snake ...201829054435
mrpl53, a new candidate gene for orofacial clefting, identified using an eqtl approach.a valuable approach to understand how individual and population genetic differences can predispose to disease is to assess the impact of genetic variants on cellular functions (e.g., gene expression) of cell and tissue types related to pathological states. to understand the genetic basis of nonsyndromic cleft lip with or without cleft palate (nscl/p) susceptibility, a complex and highly prevalent congenital malformation, we searched for genetic variants with a regulatory role in a disease-relate ...201829053389
identification of the biosynthetic pathway for the antibiotic bicyclomycin.diketopiperazines (dkps) make up a large group of natural products with diverse structures and biological activities. bicyclomycin is a broad-spectrum dkp antibiotic with unique structure and function: it contains a highly oxidized bicyclic [4.2.2] ring and is the only known selective inhibitor of the bacterial transcription termination factor, rho. here, we identify the biosynthetic gene cluster for bicyclomycin containing six iron-dependent oxidases. we demonstrate that the dkp core is made by ...201829053243
human epidermal growth factor receptor 2 as a molecular biomarker for metastatic colorectal cancer. 201829049531
polyamine-based thiols in trypanosomatids: evolution, protein structural adaptations, and biological functions.major pathogenic enterobacteria and protozoan parasites from the phylum euglenozoa, such as trypanosomatids, are endowed with glutathione (gsh)-spermidine (sp) derivatives that play important roles in signaling and metal and thiol-redox homeostasis. for some euglenozoa lineages, the gsh-sp conjugates represent the main redox cosubstrates around which entire new redox systems have evolved. several proteins underwent molecular adaptations to synthesize and utilize the new polyamine-based thiols. r ...201829048199
morphometric and morphologic parameters of the heart in healthy alouatta guariba clamitans (cabrera, 1940).this study aimed at assessing the heart function of one neotropical primate (alouatta guariba clamitans) kept in captivity using radiography, electrocardiogram (ecg) and doppler echocardiography.201829048110
antibacterial activity against cariogenic bacteria and cytotoxic and genotoxic potential of anacardium occidentale l. and anadenanthera macrocarpa (benth.) brenan extracts.the present study aimed to assess the antibacterial activity against bacteria with cariogenic relevance, toxic and genotoxic potential of the plants anacardium occidentale l. and anadenanthera macrocarpa (benth.) bernam.201829054025
modeling neuro-immune interactions during zika virus infection.although zika virus (zikv) infection is often asymptomatic, in some cases, it can lead to birth defects in newborns or serious neurologic complications in adults. however, little is known about the interplay between immune and neural cells that could contribute to the zikv pathology. to understand the mechanisms at play during infection and the antiviral immune response, we focused on neural precursor cells (npcs)-microglia interactions. our data indicate that human microglia infected with the c ...201829048558
anticancer activity of vmct1 analogs against mcf-7 cells.antimicrobial peptides are considered promising drug candidates due to their broad range of activity. vmct1 (phe-leu-gly-ala-leu-trp-asn-val-ala-lys-ser-val-phe-nh2 ) is an α-helical antimicrobial peptide that was obtained from the vaejovis mexicanus smithi scorpion venom. some of its analogs showed to be as antimicrobial as the wild type, and they were designed for understanding the influence of physiochemical parameters on antimicrobial and hemolytic activity. some cationic antimicrobial pepti ...201829044929
rhodium-induced reversible c-c bond cleavage: transformations of rhodium(iii) 22-alkyl-m-benziporphyrins.the structurally prearranged carbaporphyrins 22-methyl- and 22-ethyl-m-benziporphyrins provide the platform stabilizing aromatic rhodium(iii) 22-(μ-methylene-m-benziporphyrin) and rhodium(iii) 22-(μ-ethylidene-m-benziporphyrin). an intramolecular conversion facilitated by the m-phenylene reactivity and observed for both aromatic complexes efficiently leads to rhodium(iii) 21-(μ-methylene)-21-carbaporphyrin and rhodium(iii) 21-(μ-ethylidene)-21-carbaporphyrin. the distinctive macrocyclic environm ...201829044777
the effects of crown venting or pre-cementing of cad/cam-constructed all-ceramic crowns luted on ytz implants on marginal cement excess.the purpose of this study was to analyze the cement excess produced when cementing cad/cam-fabricated lithium disilicate (l) or zirconium dioxide (z) crowns using adhesive cement (a) or resin-modified glass ionomer cement (b). three different cementation techniques were applied: palatal venting (pv), pre-cementation with custom analogs (ca), and conventional standard procedure (sp).201829044723
animal models of neuroinflammation secondary to acute insults originated outside the brain.the term "neuroinflammation" has been widely used to describe a series of acute or chronic conditions that cause inflammation in the central nervous system (cns). neurological damage can be a consequence of direct local injury or, secondary, of systemic or even distant inflammatory processes. in this respect, animal models have been developed to better understand the pathophysiology and, possibly, to evaluate more effective methods of treatment for these disorders. animal models that promote alt ...201829044654
effectors involved in fungal-fungal interaction lead to a rare phenomenon of hyperbiotrophy in the tritrophic system biocontrol agent-powdery mildew-plant.tritrophic interactions involving a biocontrol agent, a pathogen and a plant have been analyzed predominantly from the perspective of the biocontrol agent. we have conducted the first comprehensive transcriptomic analysis of all three organisms in an effort to understand the elusive properties of pseudozyma flocculosa in the context of its biocontrol activity against blumeria graminis f.sp. hordei as it parasitizes hordeum vulgare. after inoculation of p. flocculosa, the tripartite interaction w ...201829044534
effects of loss of occlusal contact on the expression of matrix metalloproteinase-2, membrane type 1-mmp, tissue inhibitor of the mmp-2, eruption rate, organization and resistance of collagen fibers of the rat incisor periodontal ligament.this study investigated the effect of occlusal contact loss (induced by tooth shortening), on matrix metalloproteinase (mmp)-2, membrane type 1-mmp (mt1-mmp) and tissue inhibitor of the mmp-2 (timp-2) expressions in the periodontal ligament of the rat incisor, as well as eruption rate, resistance and collagen organization.201829044524
enhanced pyruvate production in candida glabrata by carrier engineering.pyruvate is an important organic acid that plays a key role in the central metabolic pathway. manipulating transporters is an efficient strategy to enhance production of target organic acids and a means to understand the effects of altered intracellular pyruvate content on global metabolic networks. efforts have been made to manipulate mitochondrial pyruvate carrier (mpc) to transport pyruvate into different subcellular compartments in candida glabrata to demonstrate the effects of the subcellul ...201829044478
exome sequencing identifies a novel nonsense mutation of myo6 as the cause of deafness in a brazilian family.we investigated 313 unrelated subjects who presented with hearing loss to identify the novel genetic causes of this condition in brazil. causative gjb2/gjb6 mutations were found in 12.7% of the patients. among the familial cases (100/313), four were selected for exome sequencing. in one case, two novel heterozygous variants were found and were predicted to be pathogenic based on bioinformatics tools, that is, p.ser906* (myo6) and p.arg42cys (gjb3). we confirmed that this nonsense myo6 mutation s ...201829044474
in vitro antiparasitic activity and chemical composition of the essential oil from protium ovatum leaves (burceraceae).leishmaniasis and trypanosomiasis are globally widespread parasitic diseases which have been responsible for high mortality rates. since drugs available for their treatment are highly hepatotoxic, nephrotoxic and cardiotoxic, adherence to therapy has been affected. thus, the search for new, more effective and safer drugs for the treatment of these diseases is necessary. natural products have stood out as an alternative to searching for new bioactive molecules with therapeutic potential. in this ...201829044326
implementation of dental specialty centers: a descriptive analysis of the current status in the brazilian territory.this study aimed to assess the current implementation status of dental specialty centers (centros de especialidades odontológicas - ceo) in brazil. the sample included ceos implemented up to november 2015 in the 27 brazilian federative units. data were obtained directly from the database of the informatics department of the brazilian unified health system, according to the national registry of health facilities (nrhf) of dental specialty centers of all brazilian regions. primary care data were a ...201829044315
evaluation of the anti-osteoporotic effect of ginkgo biloba l. in wistar rats with glucocorticoid-induced-osteoporosis by bone densitometry using dual-energy x-ray absorptiometry (dexa) and mechanical testing.evaluate the effect of the extract of ginkgo biloba in the bone alkaline phosphatase, bone mineral density, in the mechanical properties of the tibia in rats with glucocorticoid-induced-osteoporosis. after osteoporosis induction, the rats were divided into five groups: osteoporosis; egb1 (28 mg/kg); egb2 (56 mg/kg); alendronate (0.2 mg/animal) and control. the animals were treated during 20 and 30 days. the control group was compared with the osteoporosis's (student's t-test), while the other we ...201829044313
analysis of the evolution of cortical and trabecular bone compartments in the proximal femur after spinal cord injury by 3d-dxa.marked trabecular and cortical bone loss was observed at the proximal femur short-term after spinal cord injury (sci). 3d-dxa provided measurement of vbmd evolution at both femoral compartments and cortical thinning, thereby suggesting that this technique could be useful for bone analysis in these patients.201829043391
transformed follicular lymphoma.follicular lymphoma (fl) is the second most common type of non-hodgkin lymphoma and is considered to be the prototype of indolent lymphomas. histologic transformation into an aggressive lymphoma, which is expected to occur at a rate of 2 to 3% each year, is associated with rapid progression, treatment resistance, and poor prognosis. recent modifications to the physiopathologic mechanism of transformed follicular lymphoma (t-fl) have been proposed, including genetic and epigenetic mechanisms as w ...201829043381
Refresh
firstprev
...11121314151617181920...
nextlast
Displaying items 1401 - 1500 of 204098