Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| ultrastructural analysis of the interaction between f-actin and respiratory syncytial virus during virus assembly. | during respiratory syncytial virus (rsv) infection there is a close physical interaction between the filamentous actin (f-actin) and the virus, involving both inclusion bodies and the virus filaments. this interaction appears to occur relatively early in the replication cycle, and can be detected from 8 h post-infection. furthermore, during virus assembly we obtained evidence for the participation of an f-actin-associated signalling pathway involving phosphatidyl-3-kinase (pi3k). treatment with ... | 2007 | 17825340 |
| immunogenicity and efficacy of codon optimized dna vaccines encoding the f-protein of respiratory syncytial virus. | respiratory syncytial virus f-protein (rsv-f) is poorly expressed from dna expression plasmids containing the wild type rsv-f open reading frame. by codon optimization, premature polyadenylation signals were deleted and a striking enhancement of rsv-f expression levels was achieved. therefore, the immunogenicity and efficacy of wild type dna vaccines were compared to codon optimized expression plasmids encoding full-length rsv-f or its ectodomain. mice were immunized twice with the different dna ... | 2007 | 17825960 |
| the association between respiratory syncytial virus infection and the development of childhood asthma: a systematic review of the literature. | the relation between early respiratory syncytial virus (rsv) infection and later emergence of episodes of wheezing/asthma remains a subject of debate. we carried out a systematic review of studies of the association between rsv infection in the first 36 months of life and the subsequent development of asthma/bronchial hyperreactivity. | 2007 | 17848887 |
| long-lasting balanced immunity and protective efficacy against respiratory syncytial virus in mice induced by a recombinant protein g1f/m2. | respiratory syncytial virus (rsv) is the primary cause of serious lower respiratory tract illness in young children. we have engineered a recombinant candidate vaccine g1f/m2, consisting of a cytotoxic t lymphocyte (ctl) epitope of rsv-m2 protein and a domain of rsv-g protein. in this study, the long-term immunogenicity and protective effect were evaluated. in g1f/m2-immunized mice, special antibodies lasted for more than 19 weeks, and the igg1/igg2a ratio remained a balanced level till the end ... | 2007 | 17850930 |
| alphavirus replicon particles encoding the fusion or attachment glycoproteins of respiratory syncytial virus elicit protective immune responses in balb/c mice and functional serum antibodies in rhesus macaques. | respiratory syncytial virus (rsv) is a major cause of acute respiratory tract disease in humans. towards development of a prophylactic vaccine, we genetically engineered venezuelan equine encephalitis virus (veev) replicons encoding the fusion (fa) or attachment (ga or gb) proteins of the a or b subgroups of rsv. intramuscular immunization with a formulation composed of equal amounts of each replicon particle (3vrsv replicon vaccine) generated serum neutralizing antibodies against a and b strain ... | 2007 | 17850933 |
| virally delivered cytokines alter the immune response to future lung infections. | respiratory syncytial virus (rsv) is an important cause of infant morbidity and mortality worldwide and is increasingly recognized to have a role in the development and exacerbation of chronic lung diseases. there is no effective vaccine, and we reasoned that it might be possible to skew the immune system towards beneficial nonpathogenic responses by selectively priming protective t-cell subsets. we therefore tested recombinant rsv (rrsv) candidates expressing prototypic murine th1 (gamma interf ... | 2007 | 17855518 |
| multiplexed reverse transcriptase pcr assay for identification of viral respiratory pathogens at the point of care. | we have developed a nucleic acid-based assay that is rapid, sensitive, and specific and can be used for the simultaneous detection of five common human respiratory pathogens, including influenza virus a, influenza virus b, parainfluenza virus types 1 and 3, respiratory syncytial virus (rsv), and adenovirus groups b, c, and e. typically, diagnosis on an unextracted clinical sample can be provided in less than 3 h, including sample collection, preparation, and processing, as well as data analysis. ... | 2007 | 17855573 |
| the absence of enhanced disease with wild type respiratory syncytial virus infection occurring after receipt of live, attenuated, respiratory syncytial virus vaccines. | early in the development of respiratory syncytial virus (rsv) vaccines severe disease occurred in children after receipt of formalin-inactivated rsv vaccine. continuing efforts to develop an appropriately attenuated and immunogenic live rsv vaccine have given opportunities to assure that live vaccines are safe through surveillance of children after vaccination. in the present study, the rate of rsv-associated upper respiratory tract illness in 388 children was lower in rsv vaccinated children th ... | 2007 | 17868959 |
| pneumonia case-finding in the respire guatemala indoor air pollution trial: standardizing methods for resource-poor settings. | trials of environmental risk factors and acute lower respiratory infections (alri) face a double challenge: implementing sufficiently sensitive and specific outcome assessments, and blinding. we evaluate methods used in the first randomized exposure study of pollution indoors and respiratory effects (respire): a controlled trial testing the impact of reduced indoor air pollution on alri, conducted among children <or= 18 months in rural guatemala. | 2007 | 17768502 |
| [human bocavirus infections in spanish 0-14 year-old: clinical and epidemiological characteristics of an emerging respiratory virus]. | in 2005 a new respiratory virus, called human bocavirus (hbov), was cloned from respiratory samples from swedish infants and children with lower respiratory tract infections. | 2007 | 17785157 |
| inhibition of respiratory syncytial virus of subgroups a and b using deoxyribozyme dz1133 in mice. | respiratory syncytial virus (rsv) commonly infects the upper and lower respiratory tracts. currently, there is no effective treatment available. deoxyribozymes are a potential therapeutic for rsv and their activity is based on the ability to bind and cleave complementary rna sequences to inhibit protein expression. dz1133 is a deoxyribozyme that targets the conserved genomic rna sequence of the rsv nucleocapsid protein and has been shown to significantly inhibit various strains of rsv including ... | 2007 | 17804108 |
| [respiratory syncytial immunoprophylaxis with palivizumab]. | respiratory syncytial virus (rsv) is a serious pathogen causing significant morbidity, especially in premature infants and infants with chronic lung disease or significant congenital heart disease. there is no specific treatment for rsv infection and the therapy is essentially supportive. therefore, prophylaxis is the best strategy against rsv disease. passive immunization with monoclonal antibodies (palivizumab) provides protection against severe rsv infection and significantly reduces hospital ... | 2007 | 17725198 |
| palivizumab and the prevention of respiratory syncytial virus illness in pediatric patients with congenital heart disease. | respiratory syncytial virus (rsv) is a significant pathogen for infants and children with congenital heart disease. non-sustained immunity and failure to develop an effective vaccine has steered rsv management toward a passive immunotherapy strategy in at-risk children. palivizumab is a humanized murine monoclonal antibody targeting the rsv envelope f glycoprotein. in a phase iii clinical trial palivizumab significantly reduced rsv hospitalization in children with significant congenital heart di ... | 2007 | 17727335 |
| [the relationship between rsv bronchiolitis and the development of asthma]. | respiratory syncytial virus (rsv) is the most common cause of bronchiolitis during infancy and is associated with subsequent wheezing and asthma, but the nature of this association is not fully understood. in that sense, rsv bronchiolitis may serve as a marker, reflecting predisposition of the individual for virus-induced wheezing early in life and/ or asthma later in life. this review discusses existing data on rsv infection and respiratory complications later in life, as well as the link betwe ... | 2007 | 17760405 |
| nontypeable haemophilus influenzae and streptococcus pneumoniae bind respiratory syncytial virus glycoprotein. | respiratory syncytial virus (rsv) infection is associated with secondary bacterial infections caused by nontypeable haemophilus influenzae (nthi) and streptococcus pneumoniae. the pathogenesis of these complications is not completely understood; however, viral infection of respiratory epithelial cells promotes colonization by these bacteria. in the present study, rsv virions associated with nthi and pneumococci in an inoculum-dependent manner in a fluid-phase binding assay. adherence of nthi and ... | 2007 | 17761473 |
| the 24-angstrom structure of respiratory syncytial virus nucleocapsid protein-rna decameric rings. | respiratory syncytial virus (rsv), a nonsegmented, negative-sense rna-containing virus, is a common cause of lower respiratory tract disease. expression of rsv nucleocapsid protein (n) in insect cells using the baculovirus expression system leads to the formation of n-rna complexes that are morphologically indistinguishable from viral nucleocapsids. when imaged in an electron microscope, three distinct types of structures were observed: tightly wound short-pitch helices, highly extended helices, ... | 2007 | 17567697 |
| [acute bronchiolitis, an updated review]. | acute bronchiolitis (ab) is a frequent cause of hospitalization among children and its main etiological agent is respiratory syncytial virus (rsv). it occurs epidemically during autumn and winter. some populations of children such as premature newborns, infants with congenital heart disease and those with chronic lung disease, immunocompromised, undernourished, among others, present increased morbidity and mortality risk. the virus multiplies in epithelial ciliated cells while inflammation and c ... | 2007 | 17568926 |
| evaluation of compliance with palivizumab recommendations in a multicenter study of young children presenting to the emergency department with bronchiolitis. | monthly palivizumab injections from november to march decrease risk of respiratory syncytial virus (rsv)-related hospitalization during rsv season in high-risk infants born less than 35 weeks of gestation. our objective was to investigate compliance with the american academy of pediatrics (aap) recommendations for palivizumab prophylaxis among children who present to the emergency department (ed) with bronchiolitis. | 2007 | 17572518 |
| respiratory syncytial virus fusion inhibitors. part 5: optimization of benzimidazole substitution patterns towards derivatives with improved activity. | extensive sar studies and optimization of adme properties of benzimidazol-2-one derivatives led to the identification of bms-433771 (3) as an orally active rsv fusion inhibitor. in order to extend the structure-activity relationships for this compound series, substitution of the benzimidazole ring was examined with a view to establishing additional productive interactions between the inhibitor and functionality present in the proposed binding pocket. amongst the compounds synthesized, the 5-amin ... | 2007 | 17576060 |
| rsv604, a novel inhibitor of respiratory syncytial virus replication. | respiratory syncytial virus (rsv) is the most common cause of lower respiratory tract infections worldwide, yet no effective vaccine or antiviral treatment is available. here we report the discovery and initial development of rsv604, a novel benzodiazepine with submicromolar anti-rsv activity. it proved to be equipotent against all clinical isolates tested of both the a and b subtypes of the virus. the compound has a low rate of in vitro resistance development. sequencing revealed that the resis ... | 2007 | 17576833 |
| tlr4 polymorphisms mediate impaired responses to respiratory syncytial virus and lipopolysaccharide. | severe bronchiolitis following respiratory syncytial virus (rsv) infection occurs in only a small subset of infected infants and the basis for variations in disease severity is not understood. innate immune responses to rsv are mediated by tlr-4, and the (299)gly and (399)ile alleles of the tlr4 gene have been linked epidemiologically with increased severity of rsv disease in children. we hypothesized that cellular immune responses to rsv mediated by these variant forms of the receptor are defec ... | 2007 | 17579031 |
| can myoglobin expression in pancreatic beta cells improve insulin secretion under hypoxia? an exploratory study with transgenic porcine islets. | the feasibility of myoglobin (mb)-facilitated oxygen transport in improving porcine islet survival under hypoxia was investigated. discrete groups of islets were transfected with replication-defective adenoviral vector ad5 respiratory syncitial virus (rsv) to induce expression of mb or green fluorescent protein (gfp). native islets served as the controls. in vitro studies at 37 degrees c assessed islet insulin secretion efficacy: (i) to a glucose challenge from 30 to 300 mg/dl at fixed po2; and ... | 2007 | 17584476 |
| genomic analysis of rice stripe virus zhejiang isolate shows the presence of an otu-like domain in the rna1 protein and a novel sequence motif conserved within the intergenic regions of ambisense segments of tenuiviruses. | the complete genome sequence of the four rnas of rice stripe virus zhejiang isolate was determined. in addition to polymerase modules, the pc1 protein encoded on rna1 harbours an ovarian tumour (otu) - like cysteine protease signature near its n-terminus, suggesting that the protein might yield the viral polymerase and one or more additional proteins by autoproteolytic cleavage and/or have deubiquitination activity. a novel inverted repeat sequence motif was found to be universal within the inte ... | 2007 | 17585367 |
| palivizumab prophylaxis, respiratory syncytial virus, and subsequent recurrent wheezing. | children who experience respiratory syncytial virus (rsv) lower respiratory tract infections (lrtis) early in life have high rates of subsequent recurrent wheezing. palivizumab, an anti-rsv monoclonal antibody, has 78% to 80% efficacy in preventing rsv hospitalization in premature infants without chronic lung disease. we hypothesized that palivizumab, by ameliorating or preventing early rsv lrti in preterm infants, might decrease later recurrent wheezing. | 2007 | 17586188 |
| impact of human metapneumovirus and respiratory syncytial virus co-infection in severe bronchiolitis. | we have previously shown high rates of co-infection with respiratory syncytial virus (rsv) and human metapneumovirus (hmpv) in infants with severe bronchiolitis at our institution in 2000-2002, and that co-infection was associated with increased disease severity. in this study, we have attempted to identify differences in intubated infants with severe rsv infection with and without hmpv co-infection. here we show that rsv+/hmpv+ were clinically symptomatic for longer than rsv+/hmpv- infants, but ... | 2007 | 17588245 |
| respiratory syncytial virus infects and abortively replicates in the lungs in spite of preexisting immunity. | respiratory syncytial virus (rsv) is a major cause of bronchiolitis and viral pneumonia in young children and a serious health risk in immunocompromised individuals and the elderly. immunity to rsv is not completely understood. in this work, we established a method for monitoring rsv infection by real-time pcr and applied this method for analysis of rsv replication in vivo in the cotton rat model in naïve animals and in animals rendered immune to rsv by prior rsv infection. we found that even th ... | 2007 | 17596309 |
| cord blood gene expression in infants hospitalized with respiratory syncytial virus bronchiolitis. | only a few infants develop acute bronchiolitis when exposed to respiratory syncytial virus (rsv), and host, environmental, and viral properties are probably all of importance in determining the severity of infection. | 2007 | 17597454 |
| respiratory syncytial virus recombinant f protein (residues 255-278) induces a helper t cell type 1 immune response in mice. | we have developed and evaluated an immunodominant respiratory syncytial virus (rsv) f antigen in a mouse model. the antigenic region corresponding to amino acids 255-278 of the rsv f protein was cloned into a vector containing the ctxa(2)b gene of cholera toxin (ct). the recombinant protein was expressed in escherichia coli and analyzed on sodium dodecyl sulfate-polyacrylamide gels. the purified protein was evaluated by immunoblot and ganglioside gm(1) enzyme-linked immunosorbent assay to confir ... | 2007 | 17603843 |
| kinetics of antibody-induced modulation of respiratory syncytial virus antigens in a human epithelial cell line. | the binding of viral-specific antibodies to cell-surface antigens usually results in down modulation of the antigen through redistribution of antigens into patches that subsequently may be internalized by endocytosis or may form caps that can be expelled to the extracellular space. here, by use of confocal-laser-scanning microscopy we investigated the kinetics of the modulation of respiratory syncytial virus (rsv) antigen by rsv-specific igg. rsv-infected human epithelial cells (hep-2) were incu ... | 2007 | 17608950 |
| influenza and respiratory syncytial virus in infants and children: relationship with attendance at a paediatric emergency unit and characteristics of the circulating strains. | a study was carried out on 2,696 italian children, aged 0-14 years. the goals were: (1) to define the age-related impact of acute respiratory infections (ari), measured as the risk of attendance at the paediatric emergency room, (2) to better define the importance and proportion of influenza and respiratory syncytial virus (rsv) infections and (3) to acquire deeper knowledge of the influenza strains circulating in infants and children. a standardised emergency unit attendance risk (euar) was cal ... | 2007 | 17610094 |
| rapid and highly sensitive qualitative real-time assay for detection of respiratory syncytial virus a and b using nasba and molecular beacon technology. | the performance of a sensitive and specific qualitative respiratory syncytial virus (rsv) assay based on nasba technology and real-time molecular beacon detection is presented. very low detection limits for both rsv a and rsv b were determined: 95% detection hit-rate of 95 and 47 copies/input in isolation for rsv a and rsv b, respectively. rsv was detected in a wide variety of clinical samples including respiratory swabs, nasopharyngeal aspirates (npa), bronchoalveolar lavages (bal), endotrachea ... | 2007 | 17614146 |
| transmission of rice stripe virus acquired from frozen infected leaves by the small brown planthopper (laodelphax striatellus fallen). | rice stripe disease, caused by rice stripe virus (rsv), is one of the most serious rice diseases in temperate and subtropical regions of the world. since rsv is not transmissible mechanically, an insect transmission test was the original basis for identification of the viral population and cultivar resistance. a simple, rapid and reliable method is described by which virus-free small brown planthoppers acquired rsv from frozen infected rice leaves and transmitted the virus to healthy rice plants ... | 2007 | 17614147 |
| respiratory syncytial virus fusion inhibitors. part 6: an examination of the effect of structural variation of the benzimidazol-2-one heterocycle moiety. | the effect of structural variation of the benzimidazol-2-one ring of rsv fusion inhibitors related to bms-433771 (1) was examined in conjunction with side chain modifications and the introduction of an aminomethyl substituent at the 5-position of the core benzimidazole moiety. replacement of the benzimidazol-2-one moiety with benzoxazole, oxindole, quinoline-2-one, quinazolin-2,4-dione and benzothiazine derivatives provided a series of potent rsv fusion inhibitors 4. however, the intrinsic poten ... | 2007 | 17616396 |
| intratumoral expression of respiratory syncytial virus fusion protein in combination with cytokines encoded by adenoviral vectors as in situ tumor vaccine for colorectal cancer. | although cancers can naturally elicit immune responses, immune ignorance is a common observation preventing immune-mediated elimination of tumor cells. we assessed whether intratumoral expression of respiratory syncytial virus fusion (rsv-f) protein, encoded by a replication-defective adenovirus vector (ad.rsv-f), alone or in combination with local coexpression of cytokines can induce tumor-specific immune responses in a syngeneic murine colon cancer model. we confirmed in vitro by dye colocaliz ... | 2007 | 17620425 |
| respiratory syncytial virus rhinosinusitis in intensive care unit patients. | this study reported a case of rhinosinusitis for respiratory syncytial virus in intensive care unit patient. the settings were intensive care unit at hospital das clínicas, university of são paulo medical school, são paulo, brazil. one female hiv-infected patient with respiratory failure and circulatory shock due to splenic and renal abscesses, who developed rhinosinusitis caused by rsv and bacteria. respiratory viruses can play a pathogenic role in airways infection allowing secondary bacterial ... | 2007 | 17625748 |
| differential type i interferon induction by respiratory syncytial virus and influenza a virus in vivo. | type i interferon (ifn) induction is an immediate response to virus infection, and very high levels of these cytokines are produced when the toll-like receptors (tlrs) expressed at high levels by plasmacytoid dendritic cells (pdcs) are triggered by viral nucleic acids. unlike many rna viruses, respiratory syncytial virus (rsv) does not appear to activate pdcs through their tlrs and it is not clear how this difference affects ifn-alpha/beta induction in vivo. in this study, we investigated type i ... | 2007 | 17626092 |
| identification of a cd4 t cell epitope in the pneumonia virus of mice glycoprotein and characterization of its role in protective immunity. | pneumonia virus of mice (pvm) causes bronchiolitis and pneumonia in mice. infection is associated with high levels of viral replication in the lungs and results in the functional inactivation of pulmonary virus-specific cd8 t cells. due to its similarity to severe human respiratory syncytial virus (rsv) infection, pvm infection in mice has been proposed as an alternative rsv model. here, we have delineated the minimal requirements for protective t cell immunity in the pvm model. immunization wit ... | 2007 | 17632195 |
| replacement of the respiratory syncytial virus nonstructural proteins ns1 and ns2 by the v protein of parainfluenza virus 5. | paramyxoviruses have been shown to produce proteins that inhibit interferon production and signaling. for human respiratory syncytial virus (rsv), the nonstructural ns1 and ns2 proteins have been shown to have interferon antagonist activity through an unknown mechanism. to understand further the functions of ns1 and ns2, we generated recombinant rsv in which both ns1 and ns2 were replaced by the piv5 v protein, which has well-characterized ifn antagonist activities (deltans1/2-v). expression of ... | 2007 | 17632199 |
| overlapping roles of the rous sarcoma virus gag p10 domain in nuclear export and virion core morphology. | nucleocytoplasmic shuttling of the rous sarcoma virus (rsv) gag polyprotein is an integral step in virus particle assembly. a nuclear export signal (nes) was previously identified within the p10 domain of rsv gag. gag mutants containing deletions of the p10 nes or mutations of critical hydrophobic residues at positions 219, 222, 225, or 229 become trapped within the nucleus and exhibit defects in the efficiency of virus particle release. to investigate other potential roles for gag nuclear traff ... | 2007 | 17634229 |
| withdrawn: immunoglobulin for preventing respiratory syncytial virus infection. | respiratory syncytial virus, the most important cause of lower respiratory tract infections in infants and young children in industrialized countries, is associated with increased morbidity in premature infants with or without bronchopulmonary dysplasia as well as those with congenital heart disease. because of observations that lower rates of disease occur immediately after birth, presumably due to vertical transmission of maternal antibody, and animal studies where protection from pneumonia wa ... | 2007 | 17636681 |
| respiratory syncytial virus: seasonal data for regions of florida and implications for palivizumab. | the aims of this study are to define the length of the respiratory syncytial virus (rsv) season throughout florida and evaluate the american academy of pediatrics (aap) guidelines on the administration of palivizumab in light of the florida data. | 2007 | 17639745 |
| respiratory syncytial virus synergizes with th2 cytokines to induce optimal levels of tarc/ccl17. | respiratory syncytial virus (rsv) is a ubiquitous virus that preferentially infects airway epithelial cells, causing asthma exacerbations and severe disease in immunocompromised hosts. acute rsv infection induces inflammation in the lung. thymus- and activation-regulated chemokine (tarc) recruits th2 cells to sites of inflammation. we found that acute rsv infection of balb/c mice increased tarc production in the lung. immunization of balb/c mice with individual rsv proteins can lead to the devel ... | 2007 | 17641031 |
| co-infection of human metapneumovirus with adenovirus or respiratory syncytial virus among children in japan. | human metapneumovirus (hmpv) is one of the etiological agents of acute respiratory tract infections. from june 2005 to may 2006, we collected 185 clinical specimens from children in osaka city, japan, and detected 41 hmpv rna. of the 41 specimens, four (9.8%) also contained other viruses (3 with adenovirus [adv] and 1 with respiratory syncytial virus [rsv]). the clinical symptoms of patients coinfected with adv were indistinct from those of patients mono-infected with hmpv. the symptoms of the o ... | 2007 | 17641470 |
| [effect of leukotriene receptor antagonist on the levels of th1 and th2 cytokines in sera of infants with respiratory syncytial virus pneumonia]. | to investigate the effects of leukotriene receptor antagonist on the levels of th1 and th2 cytokines and the serum cysteinyl leukotrenes (cyslts) in infants and young children with respiratory syncytial virus (rsv) pneumonia. | 2007 | 17653314 |
| monoclonal antibodies in pediatrics: use in prevention and treatment. | an update is provided on monoclonal antibodies (mabs): concept, production, indications for the diagnosis and treatment of neoplastic diseases and autoimmune disorders, prevention of transplant rejection, and treatment of allergic diseases, autoimmune disease and other noninflammatory disorders such as coronary disease. mention is also made of mab use in the prevention of respiratory syncytial virus (rsv) infection. a more extensive account is provided of the use of mab in b cell lymphomas (anti ... | 2007 | 17663923 |
| characteristics and outcome of respiratory syncytial virus infection in patients with leukemia. | little is known about respiratory syncytial virus (rsv) infection in patients with leukemia. the aim of this study was to determine the characteristics, and the outcome of rsv infection with or without therapy with aerosolized ribavirin in leukemia patients. | 2007 | 17666367 |
| preventing respiratory syncytial virus in homebound premature infants. | this article explores the home health nurse's role in preventing respiratory syncytial virus (rsv) among premature infants. thousands of children infected with rsv require hospitalization each year. consistent contact with the infant alerts the nurse to subtle signs and symptoms of rsv infection, which may include nasal congestion, cough, low-grade fever, and malaise. by developing patient and caregiver trust, the home health nurse can implement an rsv prevention plan, leading to a decrease in h ... | 2007 | 17667001 |
| neurotrophins and tonsillar hypertrophy in children with obstructive sleep apnea. | enlarged adenotonsillar tissue (at) is a major determinant of obstructive sleep apnea (osa) severity in children; however, mechanisms of at proliferation are poorly understood. we hypothesized that early exposure to respiratory syncytial virus (rsv) may modify at proliferation through up-regulation of nerve growth factor (ngf)-neurokinin 1 (nk1) receptor dependent pathways. at harvested from 34 children with osa and 25 children with recurrent tonsillitis (ri) were examined for mrna expression of ... | 2007 | 17667845 |
| serine/arginine-rich proteins contribute to negative regulator of splicing element-stimulated polyadenylation in rous sarcoma virus. | rous sarcoma virus (rsv) requires large amounts of unspliced rna for replication. splicing and polyadenylation are coupled in the cells they infect, which raises the question of how viral rna is efficiently polyadenylated in the absence of splicing. optimal rsv polyadenylation requires a far-upstream splicing control element, the negative regulator of splicing (nrs), that binds sr proteins and u1/u11 snrnps and functions as a pseudo-5' splice site that interacts with and sequesters 3' splice sit ... | 2007 | 17670832 |
| immunomodulatory constituents of human milk change in response to infant bronchiolitis. | although epidemiological evidence is generally supportive of a causal association between respiratory syncytial virus (rsv) bronchiolitis during infancy and the development of persistent wheeze/asthma, if not allergy, the mechanism by which this occurs and an explanation for why all children do not succumb remains to be elucidated. breast feeding has been found to confer a protective effect against respiratory infections such as rsv bronchiolitis and allergy; however, again there is little direc ... | 2007 | 17680907 |
| respiratory syncytial virus-induced pulmonary disease and exacerbation of allergic asthma. | several respiratory viruses have been shown to cause exacerbations of asthma. while the various viral responses likely have common mechanisms of activation, the respiratory syncytial virus (rsv) appears to promote specific responses that on their own can cause severe pulmonary problems. understanding the mechanisms that promote inappropriate immune responses and local damage may lead to better therapy. the activation and recruitment of t cells that amplify and skew the immune response toward mor ... | 2007 | 17684333 |
| suppression of innate immune cytokines and interferon regulatory factor-1 by endogenous interferon-alpha in response to respiratory syncytial virus in neonate mononuclear cells. | respiratory syncytial virus (rsv) infections are extremely common in early childhood but are most severe in infants in the first few months of life. unresponsive adaptive immunity and hyporesponsive innate immunity were previously found to be the typical responses of neonate mononuclear cells (mcs) to live rsv. investigating the mechanism of innate immune hyporesponsiveness in neonate mcs to live rsv revealed that in contrast to the previously reported low expression of interferon (ifn)-gamma, i ... | 2007 | 17691025 |
| [severe bronchiolitis. epidemiology and clinical course of 284 patients]. | bronchiolitis is the leading cause of hospital admission and a frequent cause of pediatric intensive care unit (picu) admission among infants during the winter months. the objective of this study was to analyze the characteristics and clinical course of patients admitted to the picu for bronchiolitis. | 2007 | 17692256 |
| oral respiratory syncytial virus (rsv) dna vaccine expressing rsv f protein delivered by attenuated salmonella typhimurium. | human respiratory syncytial virus (rsv) is a major viral pathogen of the lower respiratory tract of infants and young children worldwide. no effective prevention measure is available. attenuated salmonella strains expressing heterologous antigens can be delivered by the oral route, triggering efficient antigen-specific humoral, cellular, and mucosal immunity. in this study, we orally administered attenuated salmonella strain sl7207, carrying the plasmid pcdna3.1/f expressing the rsv f gene, to b ... | 2007 | 17696764 |
| local immune response to respiratory syncytial virus infection is diminished in senescence-accelerated mice. | the effect of ageing on the local defence system against respiratory syncytial virus (rsv) infection was investigated using an aged mouse model of the senescence-accelerated mouse (sam) strain p1. following intranasal infection with rsv, sam-p1 mice showed a marked loss in weight, with elevated virus growth in the lungs and prolonged virus shedding. the increased susceptibility to rsv infection was associated mainly with diminished cellular immunity by local virus-specific cytotoxic t lymphocyte ... | 2007 | 17698666 |
| genetic susceptibility to respiratory syncytial virus bronchiolitis is predominantly associated with innate immune genes. | respiratory syncytial virus (rsv) is a common cause of severe lower respiratory tract infection in infants. only a proportion of children infected with rsv require hospitalization. because known risk factors for severe disease, such as premature birth, cannot fully explain differences in disease severity, genetic factors have been implicated. | 2007 | 17703412 |
| respiratory syncytial virus protects against the subsequent development of japanese cedar pollen-induced allergic responses. | respiratory syncytial virus (rsv) infection has been hypothesized to be a risk factor for the development of allergy and asthma, but epidemiologic studies in humans still remain inconclusive. the association between rsv infection and allergic diseases may be dependent on atopic background and previous history of rsv infection. in this study, the influence of the timing of rsv infection on the development of japanese cedar pollen (jcp)-induced allergic responses was examined. balb/c mice were int ... | 2007 | 17705182 |
| the use of health economics to guide drug development decisions: determining optimal values for an rsv-vaccine in a model-based scenario-analytic approach. | health-economic modelling is useful for assessing the clinical requirements and impact of new vaccines. in this study, we estimate the impact of potential vaccination for respiratory syncytial virus (rsv) of infants in the netherlands. a decision analysis model was employed using seasonal data from a cohort of children (1996-1997 through 1999-2000) to assess hospitalisation, costs and impact of vaccination. yearly, an estimated 3670 infants are hospitalised with rsv-infection in the netherlands, ... | 2007 | 17707959 |
| respiratory syncytial virus decreases p53 protein to prolong survival of airway epithelial cells. | respiratory syncytial virus (rsv) is a clinically important pathogen. it preferentially infects airway epithelial cells causing bronchiolitis in infants, exacerbations in patients with obstructive lung disease, and life-threatening pneumonia in the immunosuppressed. the p53 protein is a tumor suppressor protein that promotes apoptosis and is tightly regulated for optimal cell growth and survival. a critical negative regulator of p53 is murine double minute 2 (mdm2), an e3 ubiquitin ligase that t ... | 2007 | 17709487 |
| association of tlr4 polymorphisms with symptomatic respiratory syncytial virus infection in high-risk infants and young children. | respiratory syncytial virus (rsv) is a leading cause of infant mortality worldwide. although anti-rsv ab prophylaxis has greatly reduced infant mortality in the united states, there is currently no vaccine or effective antiviral therapy. rsv fusion (f) protein activates cells through tlr4. two single nucleotide polymorphisms (snps) encoding asp299gly and thr399ile substitutions in the tlr4 ectodomain were previously associated with tlr4 hyporesponsiveness and increased susceptibility to bacteria ... | 2007 | 17709532 |
| epidemiology of respiratory syncytial virus infection in northern taiwan, 2001-2005 -- seasonality, clinical characteristics, and disease burden. | respiratory syncytial virus (rsv) is an important pathogen in children less than 2 years old. however, there is limited epidemiological data about rsv infection in taiwan. this study aimed to investigate the clinical, epidemiological, virological, and economical aspects of rsv infections in taiwan. | 2007 | 17712463 |
| influenza- and respiratory syncytial virus-associated mortality and hospitalisations. | the aim of the current study was to estimate influenza- and respiratory syncytial virus (rsv)-associated mortality and hospitalisations, especially the influenza-associated burden among low-risk individuals < or =65 yrs old, not yet recommended for influenza vaccination in many european countries. retrospectively during 1997-2003, dutch national all-cause mortality and hospital discharge figures and virus surveillance data were used to estimate annual average influenza- and rsv-associated excess ... | 2007 | 17715167 |
| detection and quantitation of eosinophils in the murine respiratory tract by flow cytometry. | traditionally, the identification and quantification of eosinophils in inflammatory tissues and exudates has been primarily based upon morphologic criteria and manual counting. in this study, we describe a new flow cytometry-based assay to enumerate eosinophils present in murine bronchoalveolar lavage fluid (bal) and lung parenchyma obtained from the normal/non-inflamed respiratory tract, following experimentally-induced allergic pulmonary inflammation, and during experimental infection with res ... | 2007 | 17716680 |
| chemokines and their receptors in respiratory disease: a therapeutic target for respiratory syncytial virus infection. | cell recruitment is a multistep process orchestrated by chemokines and their receptors. the chemokine/receptor system is central to many inflammatory diseases, making it a key target for therapeutic intervention. despite complexity and redundancy within the system, effective antagonists are in development and undergoing clinical trials, for example, maraviroc, for use in hiv treatment. respiratory syncytial virus (rsv) is a major cause of lower respiratory tract infection in infants, with global ... | 2007 | 17547506 |
| expression of rna virus proteins by rna polymerase ii dependent expression plasmids is hindered at multiple steps. | proteins of human and animal viruses are frequently expressed from rna polymerase ii dependent expression cassettes to study protein function and to develop gene-based vaccines. initial attempts to express the g protein of vesicular stomatitis virus (vsv) and the f protein of respiratory syncytial virus (rsv) by eukaryotic promoters revealed restrictions at several steps of gene expression. | 2007 | 17550613 |
| a prospective three-year cohort study of the epidemiology and virology of acute respiratory infections of children in rural india. | acute respiratory infection (ari) is a major killer of children in developing countries. although the frequency of ari is similar in both developed and developing countries, mortality due to ari is 10-50 times higher in developing countries. viruses are common causes of ari among such children, yet the disease burden of these infections in rural communities is unknown. | 2007 | 17551572 |
| mapk and heat shock protein 27 activation are associated with respiratory syncytial virus induction of human bronchial epithelial monolayer disruption. | respiratory syncytial virus (rsv) is the major cause of bronchiolitis in infants, and a common feature of rsv infections is increased lung permeability. the accumulation of fluid in the infected lungs is caused by changes in the endothelial and epithelial membrane integrity. however, the exact mechanisms of viral-induced fluid extravasation remain unclear. here, we report that infection of human epithelial cells with rsv results in significant epithelial membrane barrier disruption as assessed b ... | 2007 | 17557802 |
| [diagnosis and epidemiological surveillance of influenza and respiratory syncytial virus infections: interest of multiplex pcr]. | respiratory infections require a rapid etiological diagnosis for efficient management of cases. we evaluated multiplex pcr used for the diagnosis and the epidemiological surveillance of influenza and respiratory syncytial virus (rsv) infections. | 2007 | 17560747 |
| intranasal proteosome-based respiratory syncytial virus (rsv) vaccines protect balb/c mice against challenge without eosinophilia or enhanced pathology. | a safe and effective vaccine against respiratory syncytial virus (rsv) is still unavailable. proteosome-based adjuvants are derived from the outer membrane proteins (omp) of neisseria species and are potent inducers of both mucosal and systemic immunity in humans and animals. candidate rsv subunit vaccines comprising enriched rsv proteins (ersv) formulated with proteosomes alone or with lps (protollin) were produced. administered intranasally in balb/c mice, both vaccines elicited long-lasting s ... | 2007 | 17561317 |
| identification of a single tryptophan residue as critical for binding activity in a humanized monoclonal antibody against respiratory syncytial virus. | we have identified a single tryptophan (trp) residue responsible for loss of binding and biological activity upon ultraviolet (uv) light irradiation in medi-493, a humanized monoclonal antibody (mab) against respiratory syncytial virus (rsv). this finding provides a better understanding of structure-function relationship in a 150-kda protein. irradiation of medi-493 with uv light resulted in spectral changes typical of trp photoproducts and in a progressive loss of medi-493 binding and biologica ... | 2007 | 17319649 |
| risk of concurrent bacterial infection in preterm infants hospitalized due to respiratory syncytial virus infection. | to evaluate the risk of concurrent bacterial infection in preterm infants hospitalized due to respiratory syncytial virus (rsv) disease. | 2007 | 17326757 |
| tap and dbp5, but not gag, are involved in dr-mediated nuclear export of unspliced rous sarcoma virus rna. | all retroviruses must circumvent cellular restrictions on the export of unspliced rnas from the nucleus. while the unspliced rna export pathways for hiv and mason-pfizer monkey virus are well characterized, that of rous sarcoma virus (rsv) is not. we have previously reported that the rsv direct repeat (dr) elements are involved in the cytoplasmic accumulation of unspliced viral rna. here, using fluorescent in situ hybridization (fish), we demonstrate that unspliced viral rnas bearing a single po ... | 2007 | 17328934 |
| human bocavirus: a novel parvovirus epidemiologically associated with pneumonia requiring hospitalization in thailand. | we detected human bocavirus (hbov) infection in 4.5% of hospitalized patients with pneumonia in rural thailand. however, the role of hbov as a pathogen is unclear. | 2007 | 17330795 |
| is clinical recognition of respiratory syncytial virus infection in hospitalized elderly and high-risk adults possible? | the clinical and radiographic features of respiratory syncytial virus (rsv) infection in elderly hospitalized persons have not been described in detail, to our knowledge, despite its relative frequent occurrence. | 2007 | 17330796 |
| eosinophil cationic protein and chemokines in nasopharyngeal secretions of infants with respiratory syncytial virus (rsv) bronchiolitis and non-rsv bronchiolitis. | bronchiolitis is a risk factor for the development of childhood asthma. eosinophilic inflammation in airways plays an important role in the pathophysiology of both bronchiolitis and asthma. to investigate this inflammation, we measured the eosinophil cationic protein (ecp), regulated on activation normal t-cell expressed and secreted (rantes) and eotaxin levels in nasopharyngeal secretions (nps). twenty-eight patients with rsv bronchiolitis (rsv group), 11 patients with non-rsv bronchiolitis (no ... | 2007 | 17297249 |
| risk factors for severe rsv-induced lower respiratory tract infection over four consecutive epidemics. | variability in severity among different respiratory syncytial virus (rsv) seasons may influence hospital admission rates for rsv-induced lower respiratory tract infection (lrti) in young children. the aim of the present study was to identify through logistic regression analysis, risk factors associated with higher likelihood to acquire rsv-induced lrti, in children with symptoms severe enough to lead to hospital admission. over four consecutive rsv seasons (2000-2004), records from children <4 y ... | 2007 | 17308898 |
| detection and typing by molecular techniques of respiratory viruses in children hospitalized for acute respiratory infection in rome, italy. | detection of a broad number of respiratory viruses is not undertaken currently for the diagnosis of acute respiratory infection due to the large and always increasing list of pathogens involved. a 1-year study was undertaken on children hospitalized consecutively for acute respiratory infection in a pediatric department in rome to characterize the viruses involved. two hundred twenty-seven children were enrolled in the study with a diagnosis of asthma, bronchiolitis, bronchopneumonia, or laringo ... | 2007 | 17311326 |
| human neutrophil elastase in rsv bronchiolitis. | acute bronchiolitis is the most common lower respiratory tract infection in young children and may be life-threatening in those with underlying cardiac or respiratory conditions. we evaluated the nasal and serum levels of human neutrophil elastase (hne) in patients with acute respiratory syncytial virus (rsv) bronchiolitis and investigated the correlation of these levels with illness severity. fifty-one patients (28 boys, 23 girls) with acute bronchiolitis positive for rsv by direct immunoenzyme ... | 2007 | 17311874 |
| cutting edge: oseltamivir decreases t cell gm1 expression and inhibits clearance of respiratory syncytial virus: potential role of endogenous sialidase in antiviral immunity. | the sialoglycosphingolipid gm1 is important for lipid rafts and immune cell signaling. t cell activation in vitro increases gm1 expression and increases endogenous sialidase activity. gm1 expression has been hypothesized to be regulated by endogenous sialidase. we tested this hypothesis in vivo using a mouse model of respiratory syncytial virus (rsv) infection. rsv infection increased endogenous sialidase activity in lung mononuclear cells. rsv infection increased lung cd8+ t cell surface gm1 ex ... | 2007 | 17312105 |
| il-13 genetic polymorphism identifies children with late wheezing after respiratory syncytial virus infection. | the nature of wheezing after respiratory syncytial virus lower respiratory tract infection (rsv lrti) is usually transient. however, some children will develop persistent or late wheezing. | 2007 | 17313976 |
| dysoxylins a-d, tetranortriterpenoids with potent anti-rsv activity from dysoxylum gaudichaudianum. | four new compounds, belonging to the tetranortriterpenoid family, named dysoxylins a-d (1-4), isolated from dysoxylum gaudichaudianum, were found to exhibit potent antiviral activity against respiratory syncytial virus (rsv). these structures were determined by nmr spectroscopy and mass spectrometry and were shown to have anti-rsv ec50 activities in the range 1.0-4.0 microg/ml in cytopathic effect inhibition and plaque reduction assays. | 2007 | 17315968 |
| does blood eosinophilia in wheezing infants predict later asthma? a prospective 18-20-year follow-up. | although eosinophilia in wheezing infants predicts subsequent wheezing, it is not known how long the association persists. our aim was to evaluate the connection between blood eosinophilia in infancy and later wheezing/asthma until adulthood, with special attention paid to infection-induced changes in blood eosinophils. we have prospectively followed up 83 infants until adulthood after hospitalization for bronchiolitis in 1981-1982. blood eosinophils were determined in a counting chamber on admi ... | 2007 | 17479599 |
| dynamics of filamentous viral rnps prior to egress. | the final step in the maturation of paramyxoviruses, orthomyxoviruses and viruses of several other families, entails the budding of the viral nucleocapsid through the plasma membrane of the host cell. many medically important viruses, such as influenza, parainfluenza, respiratory syncytial virus (rsv) and ebola, can form filamentous particles when budding. although filamentous virions have been previously studied, details of how viral filaments bud from the plasma membrane remain largely unknown ... | 2007 | 17485480 |
| human infant respiratory syncytial virus (rsv)-specific type 1 and 2 cytokine responses ex vivo during primary rsv infection. | respiratory syncytial virus (rsv) infection is the most common respiratory viral infection resulting in hospitalizations in infants worldwide. illness severity is likely multifactorial; however, unlike other viral infections, both type 1 and type 2 cytokine responses have been implicated in severe disease. | 2007 | 17492594 |
| neonatal human autologous dendritic cells pulsed with recombinant protein antigen prime the generation of non-polarized cd4 t-cell effectors. | the functional capability of human neonatal cd4 t cells to respond to vaccine antigens is frequently described as th2 biased, but whether this is due to defective t-cell or antigen-presenting cell (apc) function is unclear. in this study, we used purified t cells and autologous monocyte-derived dendritic cells (mddcs) as apcs to model primary and secondary neonatal cd4 t-cell responses in vitro to bbg2na, a recombinant protein subunit vaccine candidate against respiratory syncytial virus (rsv). ... | 2007 | 17493958 |
| function of the respiratory syncytial virus small hydrophobic protein. | respiratory syncytial virus (rsv), a member of the paramyxoviridae family, encodes a small hydrophobic (sh) protein of unknown function. parainfluenza virus 5 (piv5), a prototypical paramyxovirus, also encodes an sh protein, which inhibits tumor necrosis factor alpha (tnf-alpha) signaling. in this study, recombinant piv5 viruses without their own sh but containing rsv sh (from rsv strain a2 or b1) in its place (piv5deltash-rsv sh) and rsv lacking its own sh (rsvdeltash) were generated and analyz ... | 2007 | 17494063 |
| small interfering rna profiling reveals key role of clathrin-mediated endocytosis and early endosome formation for infection by respiratory syncytial virus. | respiratory syncytial virus (rsv) is a common cause of respiratory tract infections in infants and the elderly. like many other ph-independent enveloped viruses, rsv is thought to enter at the cell surface, independently of common endocytic pathways. we have used a targeted small interfering rna (sirna) library to identify key cellular genes involved in cytoskeletal dynamics and endosome trafficking that are important for rsv infection. surprisingly, rsv infection was potently inhibited by sirna ... | 2007 | 17494077 |
| eosinophils contribute to innate antiviral immunity and promote clearance of respiratory syncytial virus. | eosinophils are recruited to the lungs in response to respiratory syncytial virus (rsv) infection; however, their role in promoting antiviral host defense remains unclear. here, we demonstrate that eosinophils express tlrs that recognize viral nucleic acids, are activated and degranulate after single-stranded rna (ssrna) stimulation of the tlr-7-myd88 pathway, and provide host defense against rsv that is myd88 dependent. in contrast to wild-type mice, virus clearance from lung tissue was more ra ... | 2007 | 17495130 |
| antiviral activity of arbidol against influenza a virus, respiratory syncytial virus, rhinovirus, coxsackie virus and adenovirus in vitro and in vivo. | arbidol, ethyl-6-bromo-4-[(dimethylamino)-methyl]-5-hydroxy-1-methyl-2-[(phenylthio)methyl]-in dole-3-carboxylate hydrochloride monohydrate, is an antiviral chemical agent. in this report, we studied the antiviral activity of arbidol against a panel of human respiratory viruses, namely influenza a virus (flu-a, a/pr/8/34 h1n1), respiratory syncytial virus (rsv), human rhinovirus type 14 (hrv 14), coxsackie virus b3 (cvb3) and adenovirus type 7 (adv-7) in vitro in cell culture. arbidol was found ... | 2007 | 17497238 |
| surfactant protein b polymorphisms are associated with severe respiratory syncytial virus infection, but not with asthma. | surfactant proteins (sp) are important for the innate host defence and essential for a physiological lung function. several linkage and association studies have investigated the genes coding for different surfactant proteins in the context of pulmonary diseases such as chronic obstructive pulmonary disease or respiratory distress syndrome of preterm infants. in this study we tested whether sp-b was in association with two further pulmonary diseases in children, i. e. severe infections caused by ... | 2007 | 17498296 |
| vaccination with recombinant modified vaccinia virus ankara expressing bovine respiratory syncytial virus (brsv) proteins protects calves against rsv challenge. | respiratory syncytial virus (rsv) is a major cause of severe respiratory disease in infants and calves. bovine rsv (brsv) is a natural pathogen for cattle, and brsv infection in calves shares many features with the human infection. thus, brsv infection in cattle provides the ideal setting to evaluate the safety and efficacy of novel rsv vaccine strategies. here, we have evaluated the efficacy and safety of modified vaccinia virus ankara (rmva)-based vaccine candidates, expressing the bovine rsv- ... | 2007 | 17499893 |
| [sirna-mediated inhibition of respiratory syncytial virus replication]. | the effect of sirna against respiratory syncytial virus (rsv) was investigated in the cultured cells. ma104 cells were inoculated by rsv and transfected by different variants of sirna against rsv phosphoprotein (p) mrna or non-specific sirna as a control. the inhibition of rsv was assessed by microscopically studying the cells, titrating the virus, and estimating viral rna quantity in the culture supernatants by real-time polymerase chain reaction (pcr). the most potent variants of sirna caused ... | 2007 | 17500231 |
| respiratory virus infection among hematopoietic cell transplant recipients: evidence for asymptomatic parainfluenza virus infection. | the incidence of respiratory virus infection after hematopoietic cell transplantation (hct) has probably been underestimated with conventional testing methods in symptomatic patients. this prospective study assessed viral infection episodes by testing weekly respiratory samples collected from hct recipients, with and without symptoms reported by questionnaire, for 100 days after hct. samples were tested by culture and direct fluorescent antibody testing for respiratory syncytial virus (rsv), par ... | 2007 | 17502457 |
| interaction between respiratory syncytial virus and glycosaminoglycans, including heparan sulfate. | glycosaminoglycans (gags), including heparan sulfate (hs), are expressed on the surface of nearly all cells, linked to transmembrane proteins. these gags are sulfated to varying extents, lending a negative charge, and are used by a large number of viruses to initiate infection of immortalized cell lines. here we describe the rationale and methods for analyzing gag usage by one such virus, respiratory syncytial virus (rsv). the protocols presented allow the determination of which gag(s) is employ ... | 2007 | 17502668 |
| the use of two-dimensional sds-page to analyze the glycan heterogeneity of the respiratory syncytial virus fusion protein. | the respiratory syncytial virus (rsv) fusion (f) protein is synthesized as an inactive precursor (f0), which subsequently undergoes post-translational cleavage to give the disulphide bond-linked f1 and f2 subunits. the methodology detailing the use of two-dimensional electrophoresis, endoglycosidases, and alpha-mannosidase inhibitors, as applied to investigating f protein glycan maturation, is given. examples are used to show how this methodology was used to provide evidence for glycan heterogen ... | 2007 | 17502673 |
| the use of monoclonal antibodies and lectins to identify changes in viral glycoproteins that are influenced by glycosylation: the case of human respiratory syncytial virus attachment (g) glycoprotein. | the influence of viral envelope glycans is often overlooked, but one should bear in mind that variable glycosylation may affect the properties of viral envelope glycoproteins and potentially alter the course of an infection. hence, there is a need for simple methods that can be use to identify changes in the glycosylation pattern of viral glycoproteins in a large number of samples. we describe here methods for the analysis of cell-line specific changes in glycosylation of the respiratory syncyti ... | 2007 | 17502674 |
| secretion of the respiratory syncytial virus fusion protein from insect cells using the baculovirus expression system. | sequences derived from the respiratory syncytial virus (rsv) fusion (f) protein were expressed in insect cells as recombinant glutathione-s-transferase (gst)-tagged proteins. the sequence covering the f2 subunit (gst-f2), and a truncated form of the f protein in which the transmembrane domain was removed (gst-f2/f1), were cloned into the baculovirus pacsecg2t secretory vector. these virus sequences also had the endogenous virus signal sequence removed and replaced with a signal sequence derived ... | 2007 | 17502677 |
| what is the role of virus vaccination in patients with asthma? | it is estimated that viruses play a role in 30% to 80% of asthma exacerbations. thus, virus vaccination in patients with asthma could play an important role in preventing asthma exacerbations and other complications. influenza is the only agent for which a routine vaccine is currently available. this article discusses whether influenza vaccination in patients with asthma, based on the available evidence, is justified. cost-effectiveness of (influenza) vaccination for patients with asthma is ques ... | 2007 | 17504664 |
| simultaneous detection and high-throughput identification of a panel of rna viruses causing respiratory tract infections. | clinical presentations for viral respiratory tract infections are often nonspecific, and a rapid, high-throughput laboratory technique that can detect a panel of common viral pathogens is clinically desirable. we evaluated two multiplex reverse transcription-pcr (rt-pcr) products coupled with microarray-based systems for simultaneous detection of common respiratory tract viral pathogens. the ngen respiratory virus analyte-specific assay (nanogen, san diego, ca) detects influenza a virus (flu-a) ... | 2007 | 17507510 |
| [vronchiolitis and its treatment]. | bronchiolitis is the most common lower respiratory tract illness of infancy and is primarily caused by an infection with the respiratory syncytial virus (rsv). an inadequately elevated hospitalisation rate and the use of different treatments with unproven efficacy lead to high cost in terms of public health. implementation of evidence based guidelines can reduce these costs but not parents satisfaction and quality of care. palivizumab is a preventive medication with specific indications. post-br ... | 2007 | 17508665 |