Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| molecular biology of the koji molds. | 2002 | 12236056 | |
| progress of aspergillus oryzae genomics. | 2002 | 12236061 | |
| evaluation of filamentous fungi and inducers for the production of endo-polygalacturonase by solid state fermentation. | aspergillus oryzae cct 3940, aspergillus awamori nrrl 3112 and a trichoderma sp.) were compared for their capacity to produce endo-polygalacturonase (endo-pg) in solid state fermentation. maximum pectinolytic activity was reached in 72 h of growth, the best two fungal strains being a. niger t0005007-2 and a. oryzae cct 3940. three types of commercial purified pectin and four of unprocessed pectin (tangerine, orange, tahiti lime and sweet lime rind) were used to assess the effect of pectin on the ... | 2002 | 12240994 |
| transcriptional activator, aoxlnr, mediates cellulose-inductive expression of the xylanolytic and cellulolytic genes in aspergillus oryzae. | aoxlnr was isolated as a transcriptional activator of the major xylanase gene, xynf1, in aspergillus oryzae. to investigate the spectrum of genes under the control of aoxlnr, expression of the xylanolytic and cellulolytic genes in an a. oryzae wild type strain, an aoxlnr disruptant and an aoxlnr overexpressed strain was analyzed by northern blotting. aoxlnr mediated expression of at least four xylanolytic genes and four cellulolytic genes when induced by xylan and d-xylose. moreover, aoxlnr was ... | 2002 | 12297320 |
| synthesis of alpha-amylase by aspergillus oryzae in solid-state fermentation. | spent brewing grains (sbg) was evaluated for its efficacy to be used as sole carbon source for the synthesis of alpha-amylase in solid-state fermentation using a fungal strain of aspergillus oryzae nrrl 6270. enzyme production was superior when the culture grew on mesophilic temperatures and best yields were at 25 degrees c. at 30 degrees c, yields were almost comparable. maximum production of alpha-amylase [6870 u/g dry substrate (gds)] was obtained when ssf was carried out at 30 degrees c for ... | 2002 | 12362403 |
| effect of ph on simultaneous saccharification and isomerization by glucoamylase and glucose isomerase. | ph and temperature play critical roles in multistep enzymatic conversions. in such conversions, the optimal ph for individual steps differs greatly. in this article, we describe the production of glucoamylase (from aspergillus oryzae mtcc152 in solid-state fermentation) and glucose isomerase (from streptomyces griseus ncim2020 in submerged fermentation), used in industries for producing high-fructose syrup. optimum ph for glucoamylase was found to be 5.0. for glucose isomerase, the optimum ph ra ... | 2002 | 12396122 |
| production of alpha-amylase with aspergillus oryzae on spent brewing grain by solid substrate fermentation. | ten aspergillus oryzae strains were screened in solid substrate fermentation for alpha-amylase production on spent brewing grain (sbg) and on corn fiber. sbg proved to be a better substrate for enzyme production than corn fiber. a plackett-burman experimental design was used to optimize the medium composition for the best strain. solid substrate fermentation on optimized medium with a. oryzae nrrl 1808 (=atcc 12892) strain in stationary 500-ml erlenmeyer flask culture yielded 4519 u of alpha-amy ... | 2002 | 12396145 |
| determination of platinated purines in oligoribonucleotides by limited digestion with ribonucleases t1 and u2. | platinum complexes which are known to react preferentially with guanine (g) and adenine (a) bases of oligonucleotides can be used as tools to analyze their tertiary structures and eventually to cross-link them. however, this requires efficient methods to allow the identification and quantification of the corresponding adducts which have so far been developed only for oligodeoxyribonucleotides. maxam-gilbert type digestions cannot be used for rnas and hplc techniques would require too large amoun ... | 2002 | 12413471 |
| a protease-activated pathway underlying th cell type 2 activation and allergic lung disease. | the respiratory allergens that induce experimental th cell type 2-dependent allergic lung inflammation may be grouped into two functional classes. one class of allergens, in this study termed type i, requires priming with adjuvants remote from the lung to overcome airway tolerogenic mechanisms that ordinarily preclude allergic responses to inhaled ags. in contrast, the other, or type ii, allergen class requires neither remote priming nor additional adjuvants to overcome airway tolerance and elic ... | 2002 | 12421974 |
| differentiation of feruloyl esterases on synthetic substrates in alpha-arabinofuranosidase-coupled and ultraviolet-spectrophotometric assays. | 4-nitrophenyl 5-o-trans-feruloyl-alpha-l-arabinofuranoside and 4-nitrophenyl 2-o-trans-feruloyl-alpha-l-arabinofuranoside, synthesized by our group (m. mastihubová, j. szemesová, and p. biely), were found to be suitable substrates for determination of activity of feruloyl esterases (fees) exhibiting affinity for 5-o- and 2-o-feruloylated alpha-l-arabinofuranosyl residues. one assay is based on coupling the fee-catalyzed formation of 4-nitrophenyl alpha-l-arabinofuranoside with its efficient hydr ... | 2002 | 12441154 |
| cloning and characterization of the naga gene that encodes beta-n-acetylglucosaminidase from aspergillus nidulans and its expression in aspergillus oryzae. | we isolated a beta-n-acetylglucosaminidase encoding gene and its cdna from the filamentous fungus aspergillus nidulans, and designated it naga. the naga gene contained no intron and encoded a polypeptide of 603 amino acids with a putative 19-amino acid signal sequence. the deduced amino acid sequence was very similar to the sequence of candida albicans hex1 and trichoderma harzianum nag1. yeast cells containing the naga cdna under the control of the gal1 promoter expressed beta-n-acetylglucosami ... | 2002 | 12450128 |
| antifungal activity of substituted 8-quinolinol-5- and 7-sulfonic acids: a mechanism of action is suggested based on intramolecular synergism. | 8-quinolinol-5-sulfonic acid was nearly devoid of antimicrobial activity, due to what was believed to be an unfavorable partition coefficient. since twenty six 8-quinolinol-5- and 7-sulfonic acids were available from our previous work, they were tested against six fungi. the 7-chloro and 7-bromo-5-sulfonic acids and the 5-chloro and 5-bromo-7-sulfonic acids showed fungal inhibition within one order of magnitude of that of 8-quinolinol. it is suggested that a nonchelating mechanism is in part res ... | 2002 | 12650598 |
| cloning, nucleotide sequencing, and expression of the beta-galactosidase-encoding gene (laca) from aspergillus oryzae. | laca coding for beta-galactosidase (beta-gal) was cloned from the genomic dna of aspergillus oryzae rib40. there were 9 exons in laca and the coding region of 3,015 bp encoded a protein of 1,005 aa with a deduced molecular mass of 109,898. a. oryzae laca was highly homologous to fungal beta-gals, with the highest aa identity of 70.7% to a. niger laca, and also showed significant identity to acid beta-gals belonging to family 35 glycosyl hydrolases. approximately 10 copies of laca under control o ... | 2002 | 12469296 |
| cloning and nucleotide sequence of the glutamate decarboxylase-encoding gene gada from aspergillus oryzae. | we cloned a genomic dna encoding the glutamate decarboxylase (gad) from aspergillus oryzae using a 200-bp dna fragment as the probe. this dna fragment was amplified by the reverse transcription polymerase chain reaction with mrna of a. oryzae as the template and degenerate primers designed from the conserved amino acid sequence of escherichia coli gad and arabidopsis thaliana gad. nucleotide sequencing analysis showed that the cloned gene (designated gada) encoded 514 amino acid residues and con ... | 2002 | 12596854 |
| [improvement of recombinant xylanase xynf1 from aspergillus oryzae expressed in pichia pastoris by site-directed mutagenesis]. | the recombinant xylanase xynf1 from aspergillus oryzae expressed in the methylotrophic yeast pichia pastoris was improved by site-directed mutagenesis. the mutant i156a secreted about 47.7 u/ml xylanase xynf1 in the bmmy medium. the mutant xylanase xynf1 was purified by ion exchange and gel filtration chromatographies. the molecular weight of purified xynf1 was 35 kd. xynf1 was stable between ph 4-9 and its optimum ph was 7.0. the optimum temperature of xynf1 was 45 degrees c and it was stable b ... | 2002 | 12557547 |
| [cloning and sequencing of lactase gene from aspergillus candidus]. | genomic dna and cdna sequences of lactase from aspergillus candidus were cloned. sequences analysis revealed that the genomic dna was 3458 bp containing eight introns, cdna was 3015 bp and encoding a polypeptide of 1005 amino acid residues. signal peptide was 19 amino acid residues, eleven potential n-glycosylation sites were assumed. comparing the gene cdna and amino acid sequences with other lactase sequences from various sources, it showed a very low homology with most of other sequences. alt ... | 2002 | 12561200 |
| protein expression and secretion in the yeast yarrowia lipolytica. | strains and vectors for protein expression and secretion have been developed in the yeast yarrowia lipolytica. host strains were constructed with non-reverting auxotrophic markers, deletions of protease-encoding genes, and carrying a docking platform. to drive transcription, either the synthetic hp4d or the inducible pox2 promoter were used. protein secretion is either directed by the targeting sequence of the alkaline extracellular protease or the extracellular lipase (lip2p) signal sequence. w ... | 2002 | 12702287 |
| aspergillus oryzae in solid-state and submerged fermentations. progress report on a multi-disciplinary project. | we report the progress of a multi-disciplinary research project on solid-state fermentation (ssf) of the filamentous fungus aspergillus oryzae. the molecular and physiological aspects of the fungus in submerged fermentation (smf) and ssf are compared and we observe a number of differences correlated with the different growth conditions. first, the aerial hyphae which occur only in ssfs are mainly responsible for oxygen uptake. second, ssf is characterised by gradients in temperature, water activ ... | 2002 | 12702312 |
| physiological characterisation of recombinant aspergillus nidulans strains with different crea genotypes expressing a. oryzae alpha-amylase. | the physiology of three strains of aspergillus nidulans was examined--a crea deletion strain, a wild type crea genotype and a strain containing extra copies of the crea gene, all producing aspergillus oryzae alpha-amylase. the strains were cultured in batch and continuous cultivations and the biomass formation and alpha-amylase production was characterised. overexpression of the crea gene resulted in a lower maximum specific growth rate and a slightly higher repression of the alpha-amylase produ ... | 2002 | 11689252 |
| establishment of a hyper-protein production system in submerged aspergillus oryzae culture under tyrosinase-encoding gene (melo) promoter control. | uv-mediated mutagenesis generated a high glucoamylase-producing mutant of aspergillus oryzae exhibiting strong melanization in solid-state culture. expression of the glucoamylase-encoding gene (glab), which is specifically expressed in solid-state culture, and the tyrosinase-encoding gene (melo), was analyzed using an e. coli beta-glucuronidase (gus) reporter assay to investigate this phenomenon. although no common regulation was found for melo and glab expression, the former was greatly enhance ... | 2001 | 11693910 |
| electrophoretic mobility shift scanning using an automated infrared dna sequencer. | electrophoretic mobility shift assay (emsa) is widely used in the study of sequence-specific dna-binding proteins, including transcription factors and mismatch binding proteins. we have established a non-radioisotope-based protocol for emsa that features an automated dna sequencer with an infrared fluorescent dye (irdye) detection unit. our modification of the elec- trophoresis unit, which includes cooling the gel plates with a reduced well-to-read length, has made it possible to detect shifted ... | 2001 | 11730013 |
| cloning and expression in phospholipid containing cultures of the gene encoding the specific phosphatidylglycerol/phosphatidylinositol transfer protein from aspergillus oryzae: evidence that the pg/pi-tp is tandemly arranged with the putative 3-ketoacyl-coa thiolase gene. | the phosphatidylglycerol/phosphatidylinositol transfer protein (pg/pi-tp) is a new and original phospholipid transfer protein (pltp) isolated from the deuteromycete, aspergillus oryzae. we have isolated a genomic clone of the a. oryzae pg/pi-tp using a probe derived from the corresponding cdna and sequenced the complete gene. the dna sequence analysis revealed that pg/pi-tp gene is composed of three exons encoding a 18,823 da protein of 175 amino acids as previously described and of two introns ... | 2001 | 11179668 |
| aohapb, aohapc and aohape, subunits of the aspergillus oryzae ccaat-binding complex, are functionally interchangeable with the corresponding subunits in aspergillus nidulans. | two genes, aohapb and aohape, encoding subunits of the aspergillus oryzae ccaat-binding complex were cloned and sequenced. the polypeptides encoded by aohapb and aohape were expressed in escherichia coli and used to reconstitute a dna-binding complex with recombinant aohapc. the dna-binding activity was observed only in the presence of all three subunits, indicating that aohapb, aohape and aohapc are essential for ccaat-binding. furthermore, introduction of the aohapb, aohapc and aohape genes in ... | 2001 | 11409179 |
| enzymatic synthesis of two ginsenoside re-beta-xylosides. | two new beta-xylosyl derivatives of ginsenoside re, 20(s)-protopanaxatriol 6-o-alpha-l-rhamnopyranosyl-(1 --> 2)-[beta-d-xylopyranosyl-(1 --> 4)]-beta-d-glucopyranosyl-20-o-beta-d-glucopyranoside and 20(s)-protopanaxatriol 6-o-alpha-l-rhamnopyranosyl-(1 --> 2)-[beta-d-xylopyranosyl-(1 --> 6)]-beta-d-glucopyranosyl-20-o-beta-d-glucopyranoside, were respectively synthesized from p-nitrophenyl beta-d-xylopyranoside and phenyl beta-d-xylopyranoside as donors and ginsenoside re as the acceptor in 25% ... | 2001 | 11440145 |
| [mystery of fermented foods--there are friendly molds too!]. | 2001 | 11441805 | |
| functional analysis of tama, a coactivator of nitrogen-regulated gene expression in aspergillus nidulans. | the tam a gene of aspergillus nidulans encodes a 739-amino acid protein with similarity to uga35p/dal81p/durlp of saccharomyces cerevisiae. it has been proposed that tama functions as a co-activator of area, the major nitrogen regulatory protein in a. nidulans. because area functions as a transcriptional activator under nitrogen-limiting conditions, we investigated whether tama was also present in the nucleus. we found that a gfp-tama fusion protein was predominantly localised to the nucleus in ... | 2001 | 11459183 |
| screening and characterization of koji molds producing saline-tolerant protease. | three mold strains isolated from soil in the taipei area of taiwan were compared with a commercial strain of aspergillus oryzae for their proteolytic activities in an 18% nacl aqueous solution system. among these strains, the one subsequently identified and designated as aspergillus sp. fc-10 produced protease with superior saline tolerance. in aflatoxin tests, this strain did not generate detectable aflatoxin after growing on steamed grain polished rice substrate for 24 days. two types of extra ... | 2001 | 11464272 |
| bi-fluorescence-labeled maltoheptaoside: convenient substrate for continual assay of alpha-amylase. | a new maltoheptaose derivative was prepared as a useful substrate for continual assay of alpha-amylase. the maltoheptaoside has thionaphtyl group as a fluorescent energy donor at the reducing end and dansyl group as an acceptor group at the non-reducing end. excitation of the thionaphthyl group at 290 nm results in emission at 523 nm from the dansyl group, while the emission from the thionaphthyl group is quenched by the dansyl group. this fluorescence energy transfer is reduced by the hydrolyti ... | 2001 | 11506161 |
| isoflavone transformation during soybean koji preparation and subsequent miso fermentation supplemented with ethanol and nacl. | soybeans were soaked with water for 4 h, steam-cooked, inoculated with the conidia of aspergillus oryzae, and incubated for 3 days for koji preparation. the koji was then mixed with water-soaked and steam-cooked soybeans (1:2, w/w), ground into paste, and supplemented with 15% ethanol and 12.5% nacl or 3% ethanol and 6% nacl for miso fermentation at 30 degrees c. daidzin, genistin, daidzein, and genistein contents were extracted from the lyophilized and pulverized soybean powder or from the miso ... | 2001 | 11513643 |
| visualization of nuclei in aspergillus oryzae with egfp and analysis of the number of nuclei in each conidium by facs. | aspergillus oryzae has been reported to form conidia with multinuclei. in order to analyze nuclei in living cells, we developed an expression system of the a. nidutans histone h2b protein tagged by egfp (h2b::egfp). in both a. oryzae niad300 and a. nidulans fgsc89 transformants expressing h2b::egfp, fluorescence was detected in nuclear regions of hyphae and conidia. while a conidium contained only one fluorescent spot in the a. nidulans transformant, approximately 66% of conidia had two, 24% had ... | 2001 | 11515532 |
| a new class of glutaminase from aspergillus oryzae. | the koji mold aspergillus oryzae is able to produce glutaminase which converts glutamine to glutamic acid, one of the most important flavor components in soy sauce. we present here the isolation and the complete nucleotide sequence of the glutaminase- encoding gene from a. oryzae u212, an industrial strain used in thailand. n-terminal and internal amino acid sequences were determined from purified glutaminase. a 700-bp fragment was amplified by pcr using oligonucleotide primers designed from par ... | 2001 | 11545278 |
| increased phospholipid transfer protein activity in aspergillus oryzae grown on various industrial phospholipid sources. | the effect of industrial carbon sources on phospholipid transfer protein production was investigated. phospholipid fractions of different composition were prepared from various plant oils (i.e., soybean, rapeseed, and sunflower) according to the lucas meyer extraction and purification process. the effect of these fractions on phospholipid transfer protein activity of cell extracts from aspergillus oryzae grown on medium containing these phospholipids as sole carbon source was studied. it was sho ... | 2001 | 11547891 |
| on the involvement of electron transfer reactions in the fluorescence decay kinetics heterogeneity of proteins. | time-resolved fluorescence study of single tryptophan-containing proteins, nuclease, ribonuclease t1, protein g, glucagon, and mastoparan, has been carried out. three different methods were used for the analysis of fluorescence decays: the iterative reconvolution method, as reviewed and developed in our laboratory, the maximum entropy method, and the recent method that we called "energy transfer" method. all the proteins show heterogeneous fluorescence kinetics (multiexponential decay). the orig ... | 2001 | 11567101 |
| expression, gene cloning, and characterization of five novel phytases from four basidiomycete fungi: peniophora lycii, agrocybe pediades, a ceriporia sp., and trametes pubescens. | phytases catalyze the hydrolysis of phosphomonoester bonds of phytate (myo-inositol hexakisphosphate), thereby creating lower forms of myo-inositol phosphates and inorganic phosphate. in this study, cdna expression libraries were constructed from four basidiomycete fungi (peniophora lycii, agrocybe pediades, a ceriporia sp., and trametes pubescens) and screened for phytase activity in yeast. one full-length phytase-encoding cdna was isolated from each library, except for the ceriporia sp. librar ... | 2001 | 11571175 |
| fluorescence polarization: analysis of carbohydrate-protein interaction. | fluorescence polarization has been widely used for the studies on the molecular motion in solution and has been applied to immunoassays for proteins, therapeutic drug monitoring in clinical pharmacy, and assays for environmentally toxic compounds. because fluorescence polarization is most readily applicable to the kinetic analysis of the binding reaction between a substance having small molecular mass and a receptor molecule, this method is easily applied to the analysis of carbohydrate-lectin b ... | 2001 | 11673876 |
| identification of catalytic and substrate-binding site residues in bacillus cereus atcc7064 oligo-1,6-glucosidase. | three active site residues (asp199, glu255, asp329) and two substrate-binding site residues (his103, his328) of oligo-1,6-glucosidase (ec 3.2.1.10) from bacillus cereus atcc7064 were identified by site-directed mutagenesis. these residues were deduced from the x-ray crystallographic analysis and the comparison of the primary structure of the oligo-1,6-glucosidase with those of saccharomyces carlsbergensis alpha-glucosidase, aspergillus oryzae alpha-amylase and pig pancreatic alpha-amylase which ... | 2001 | 11676021 |
| a quick solution: ab initio structure determination of a 19 kda metalloproteinase using acorn. | a data set from the metalloproteinase deuterolysin was collected at atomic resolution (1.0 a) with synchrotron radiation. the high resolution allowed the structure to be solved with the new direct-methods program acorn using the coordinates of the zn atom as a starting point. the phases obtained from acorn were of sufficient quality to allow automated building to be carried out in arp/warp. minimal manual rebuilding of the model was required and the structure determination was completed using th ... | 2001 | 11679721 |
| cloning and sequence analysis of cnaa gene encoding the catalytic subunit of calcineurin from aspergillus oryzae. | calcineurin has been implicated in ion-homeostasis, stress adaptation in yeast and for hyphal growth in filamentous fungi. genomic dna and cdna encoding the catalytic subunit of calcineurin (cnaa) were isolated from aspergillus oryzae. the cnaa open reading frame extended to 1727 bp and encoded a putative protein of 514 amino acids. comparative analysis of the nucleotide sequence of cnaa genomic dna and cdna confirmed the presence of three introns and a highly conserved calmodulin binding domain ... | 2001 | 11682197 |
| temperature control in a continuously mixed bioreactor for solid-state fermentation. | a continuously mixed, aseptic paddle mixer was used successfully for solid-state fermentation (ssf) with aspergillus oryzae on whole wheat kernels. continuous mixing improved temperature control and prevented inhomogeneities in the bed. respiration rates found in this system were comparable to those in small, isothermal, unmixed beds, which showed that continuous mixing did not cause serious damage to the fungus or the wheat kernels. continuous mixing improves heat transport to the bioreactor wa ... | 2001 | 11114659 |
| model for on-line moisture-content control during solid-state fermentation. | in this study we describe a model that estimates the extracellular (nonfungal) and overall water contents of wheat grains during solid-state fermentation (ssf) with aspergillus oryzae, using on-line measurements of oxygen, carbon dioxide, and water vapor in the gas phase. the model uses elemental balances to predict substrate dry matter losses from carbon dioxide measurements, and metabolic water production, water used in starch hydrolysis, and water incorporated in new biomass from oxygen measu ... | 2001 | 11114660 |
| alpha-amylase production in high cell density submerged cultivation of aspergillus oryzae and a. nidulans. | the effect of biomass concentration on the formation of aspergillus oryzae alpha-amylase during submerged cultivation with a. oryzae and recombinant a. nidulans strains has been investigated. it was found that the specific rate of alpha-amylase formation in chemostats decreased significantly with increasing biomass concentration in the range of approx. 2-12 g dry weight kg(-1). when using a recombinant a. nidulans strain in which the gene responsible for carbon catabolite repression of the a. or ... | 2001 | 11234963 |
| the aspergillus nidulans multimeric ccaat binding complex ancf is negatively autoregulated via its hapb subunit gene. | cis-acting ccaat elements are frequently found in eukaryotic promoter regions. many of them are bound by conserved multimeric complexes. in the fungus aspergillus nidulans the respective complex was designated ancf (a. nidulans ccaat binding factor). ancf is composed of at least three subunits designated hapb, hapc and hape. here, we show that the promoter regions of the hapb genes in both a. nidulans and aspergillus oryzae contain two inversely oriented, conserved ccaat boxes (box alpha and box ... | 2001 | 11243777 |
| a cardinal model to describe the effect of water activity on the growth of moulds. | a simple model was proposed to describe the effect of water activity (aw) on the radial growth rate of moulds. this model is deduced from the cardinal model family proposed by rosso in 1995, which is only defined from cardinal values of environmental factors (minimum, optimum and maximum values), the growth rate observed at the optimal value of the environmental factors, and n, a shape parameter. for aw, a simple form of cardinal model is proposed. this form is obtained for n = 2 and aw(max) = 1 ... | 2001 | 11246910 |
| sequence analysis and overexpression of a pectin lyase gene (pel1) from aspergillus oryzae kbn616. | a gene (pel1) encoding pectin lyase (pel1) was isolated from a shoyu koji mold, aspergillus oryzae kbn616, and characterized. the structural gene comprised 1,196 bp with a single intron. the orf encoded 381 amino acids with a signal peptide of 20 amino acids. the deduced amino acid sequence showed high similarity to those of aspergillus niger pectin lyases and glomerella cingulata pnla. the pel1 gene was successfully overexpressed under the promoter of the a. oryzae tef1 gene. the molecular mass ... | 2001 | 11272833 |
| high-level secretory production of phospholipase a1 by saccharomyces cerevisiae and aspergillus oryzae. | phospholipase a1 (pla1) is a hydrolytic enzyme that catalyzes the removal of the acyl group from position 1 of lecithin to form lysolecithin. the pla1 gene, which had been cloned from aspergillus oryzae, was expressed in saccharomyces cerevisiae and a. oryzae. through the modification of the medium composition and the feeding conditions of substrate, the production level of pla1 by s. cerevisiae was increased to a level fivefold higher than that indicated in a previous report. in the case of a. ... | 2001 | 11272851 |
| synergistic action of an x-prolyl dipeptidyl aminopeptidase and a non-specific aminopeptidase in protein hydrolysis. | non-specific monoaminopeptidase (ap; e.c. 3.4.11) and x-prolyl dipeptidyl aminopeptidase (x-pdap; e.c. 3.4.14.5), both from aspergillus oryzae, demonstrate strong synergism in hydrolyzing proline-containing peptides. incubation of ap alone with the peptide ala-pro-gly-asp-arg-ile-tyr-val-his-pro-phe does not generate free amino acids. however, when ap and x-pdap are added in combination, complete and immediate hydrolysis of all peptide bonds, other than x-pro bonds, is observed. in the enzymatic ... | 2001 | 11308367 |
| isoenzyme multiplicity and characterization of recombinant manganese peroxidases from ceriporiopsis subvermispora and phanerochaete chrysosporium. | we expressed cdnas coding for manganese peroxidases (mnps) from the basidiomycetes ceriporiopsis subvermispora (mnp1) and phanerochaete chrysosporium (h4) under control of the alpha-amylase promoter from aspergillus oryzae in aspergillus nidulans. the recombinant proteins (rmnp1 and rh4) were expressed at similar levels and had molecular masses, both before and after deglycosylation, that were the same as those described for the mnps isolated from the corresponding parental strains. isoelectric ... | 2001 | 11319083 |
| enzymatic glycosylation using 6-o-acylated sugar donors and acceptors: beta-n-acetylhexosaminidase-catalysed synthesis of 6-o,n,n'-triacetylchitobiose and 6'-o,n,n'-triacetylchitobiose. | p-nitrophenyl 6-o-acetyl-2-acetamido-2-deoxy-beta-d-glucopyranoside (5a) was used as the glycosyl donor in a beta-n-acetylhexosaminidase-catalysed (from penicillium brasilianum) glycosylation of glcnac yielding 6'-o,n,n'-triacetylchitobiose (6), while 6-o-acetyl-2-acetamido-2-deoxy-beta-d-glucopyranose (3a) served as a selectively protected acceptor in a transglycosylation reaction catalysed by the same enzyme to yield 6-o,n,n'-triacetylchitobiose (4). | 2001 | 11322728 |
| stimulation of myelopoiesis in listeria monocytogenes-infected mice by an aggregated polymer isolated from aspergillus oryzae. | in this work, we investigated the effects of the proteic aggregated polymer of magnesium ammonium phospholinoleate-palmitoleate anhydride (mapa) isolated from aspergillus oryzae on the growth and differentiation of bone marrow granulocyte-macrophage progenitor cells (cfu-gm) in listeriamonocytogenes-infected mice. a significant reduction in the cfu-gm number was observed in the initial phase of infection with a sublethal dose of listeria. treatment of mice with 0.5, 2.0 and 5.0 mg/kg mapa for 7 ... | 2001 | 11339624 |
| cloning and characterization of a chitosanase gene from the koji mold aspergillus oryzae strain iam 2660. | a genomic copy of the gene coding for chitosanase (csna) was isolated from aspergillus oryzae iam 2660. a. oryzae csna contains an open reading frame that encodes a polypeptide of 245 amino acids with a calculated molecular mass of 26,500 da. the deduced amino acid sequence of a. oryzae csna indicates extensive similarities to those of other fungal chitosanases. | 2001 | 11388486 |
| production and properties of beta-mannanase by free and immobilized cells of aspergillus oryzae nrrl 3488. | seven fungi were tested for production of mannanases. the highest mannanase activities were produced by aspergillus oryzae nrrl 3488 after 7 days in static cultures. mannanases were induced by gum locust bean (1.0%). the highest mannanase activity was produced when a mixture of peptone, urea and ammonium sulphate was used as nitrogen source. zn2+ or co2+ favoured enzyme production. the immobilized cells on ca-alginate and agar were able to produce beta-mannanase for four runs with a slight decre ... | 2001 | 11393772 |
| deletion analysis of the enolase gene (enoa) promoter from the filamentous fungus aspegillus oryzae. | the enolase gene (enoa) is one of the most strongly expressed genes in aspergillus oryzae. to elucidate the transcription regulatory element for this strong expression and the process of glucose induction, the transcription activity of a series of truncated enoa promoters was measured by using the escherichia coli uida gene as a reporter. deletion of a 104-bp region located -224 nt to -121 nt upstream of the translation initiation site caused both a drastic decrease in the beta-glucuronidase (gu ... | 2001 | 11795846 |
| plasminogen-binding activity of enolase in the opportunistic pathogen pneumocystis carinii. | the glycolytic enzyme enolase is one of the most abundant proteins expressed in fungi and has been shown to be an immunodominant cell-wall-associated antigen of the pathogenic fungus, candida albicans. enolase has also been found on the surface of some mammalian cells where it functions as a plasminogen-binding motif and facilitator of plasminogen activation to plasmin. to investigate the immunogenicity of enolase in the opportunistic pathogen, pneumocystis carinii, the genomic and complementary ... | 2001 | 11798055 |
| influence of carbon source on alpha-amylase production by aspergillus oryzae. | the influence of the carbon source on alpha-amylase production by aspergillus oryzae was quantified in carbon-limited chemostat cultures. the following carbon sources were investigated: maltose, maltodextrin (different chain lengths), glucose, fructose, galactose, sucrose, glycerol, mannitol and acetate. a. oryzae did not grow on galactose as the sole carbon source, but galactose was co-metabolized together with glucose. relative to that on low glucose concentration (below 10 mg/l), productivity ... | 2001 | 11759683 |
| a novel thermostable branching enzyme from an extremely thermophilic bacterial species, rhodothermus obamensis. | a branching enzyme (ec 2.4.1.18) gene was isolated from an extremely thermophilic bacterium, rhodothermus obamensis. the predicted protein encodes a polypeptide of 621 amino acids with a predicted molecular mass of 72 kda. the deduced amino acid sequence shares 42-50% similarity to known bacterial branching enzyme sequences. similar to the bacillus branching enzymes, the predicted protein has a shorter n-terminal amino acid extension than that of the escherichia coli branching enzyme. the deduce ... | 2001 | 11778874 |
| a new flavone glycoside, 5-hydroxy 7,3',4',5'-tetra-methoxyflavone 5-o-beta-d-xylopyranosyl-(1-->2)-alpha-l-rhamnopyranoside from bauhinia variegata linn. | a new flavone glycoside m.f. c(30)h(36)o(15) m.p. 252-253 degrees c, [m]+ 636 (eims) was isolated from the acetone soluble fraction of the concentrated 95% ethanolic extract of the seeds of bauhinia variegata (linn). it was identified as 5-hydroxy7,3',4',5'-tetra-methoxyflavone 5-o-beta-d-xylopyranosyl-(1-->2)-alpha-l-rhamnopyranoside (1) by various colour reactions, chemical degradations and spectral techniques. | 2001 | 11783588 |
| a new biologically active triterpenoid saponin from the leaves of lepidagathis hyalina nees. | a new biologically active triterpenoid saponin m.f. c42h68o13, m.p. 315 degrees-317 degrees c was isolated from the ethyl acetate soluble fraction of the methanolic extract of the leaves of lepidagathis hyalina. its structure was characterized as 3-beta-o-[alpha-l-rhamnopyranosyl(1-->4)o-beta-d-glucopyranosyl]16-alpha-hydroxy-olean-12-en(13)-28-oic acid by several spectral and chemical analysis. this new triterpenoid saponin showed antimicrobial activity against various plants pathogenic bacteri ... | 2001 | 11841115 |
| improvement of production of kojic acid by a mutant strain aspergillus oryzae, mk107-39. | a strain designated mk107-39, producing kojic acid with a high yield, was obtained by a new screening method using a 96-well microtiter plate after ntg treatment of aspergillus oryze atcc 22788. the amount of kojic acid produced by strain mk107-39 in a shaking flask was 28 g/l from 100 g/l of glucose, which was 7.7-times higher than that produced by parent strain. the kojic acid yields per cell and the amount of glucose consumed were 9.8 and 6.0-times higher than those of the parent strain. base ... | 2001 | 16232988 |
| a second pectin lyase gene (pel2) from aspergillus oryzae kbn616: its sequence analysis and overexpression, and characterization of the gene products. | a second pectin lyase gene, designated pel2, was isolated from a shoyu koji mold aspergillus oryzae kbn616 and characterized. the structural gene comprised 1306 bp with three introns. the orf encoded 375 amino acids with a signal peptide of 19 amino acids. the deduced amino acid sequence showed high similarity to those of a. oryzae pel1, aspergillus niger pectin lyases and glomerella cingulata pn1a. the pel2 gene was overexpressed under the control of the promoter of the a. oryzae tef1 gene for ... | 2001 | 16233008 |
| overexpression and purification of aspergillus aculeatus beta-mannosidase and analysis of the integrated gene in aspergillus oryzae. | an expression plasmid for the manb gene encoding aspergillus aculeatus beta-d-mannosidase (manb) was constructed by using an expression vector carrying an improved promoter. after transformation of a. oryzae by the plasmid, several transformants formed colonies emitting fluorescence on a plate containing 4-methylumbelliferyl beta-d-mannopyranoside (mu-man) under uv-irradiation. the transformant that displayed the strongest fluorescence, named a. oryzae bmn1, produced about 270 mg manb/l in liqui ... | 2001 | 16233072 |
| kojic acid production in an airlift bioreactor using partially hydrolyzed raw corn starch. | in a culture of aspergillus oryzae mk-107-39 in a 3-l airlift bioreactor, kojic acid was not produced when glucose/wheat germ medium (gm1) was used. however, when a jar fermentor was used, the kojic acid yield was high. a suitable medium for culture in an airlift bioreactor consisting of partially hydrolyzed corn starch and a small amount of corn steep liquor (csl) (sm1) was selected. in the cultivation in the airlift bioreactor using sm1, nearly 40 g/l of kojic acid was produced, which was the ... | 2001 | 16233111 |
| efficient production of a heterologous peroxidase, dyp from geotrichum candidum dec 1, on solid-state culture of aspergillus oryzae rd005. | the productivity of a peroxidase (dyp) originating from geotrichum candidum dec 1 was enhanced in the solid-state culture using aspergillus oryzae rd005. when the humidity, water content, and temperature were adjusted to 60%, 50% and 27 degrees c, respectively, the productivity of dyp reached 5.3 g per kilogram wheat bran, which was used as the solid medium. the yield of 5.3 g per kg wheat bran corresponded to the yield of a 56 kg submerged culture. the productivity per gram carbon of the medium ... | 2001 | 16233153 |
| a new xylanase from penicillium griseofulvum. | a new xylanase (pgxyna) from penicillium griseofulvum a160 has been isolated and characterised using a screening method based on the ability to digest a complex substrate. the enzyme belongs to the hydrolase family 11 or g and shows an optimum ph of 5.0 and an optimum temperature of 50 degrees c. the xylanase breaks down the xylan to very small oligosaccharides. the corresponding gene (pgxyna) was cloned and expressed in aspergillus oryzae. a second xylanase gene with 66% identity to pgxyna has ... | 2001 | 15954597 |
| molecular cloning of the isoamyl alcohol oxidase-encoding gene (mrea) from aspergillus oryzae. | isoamyl alcohol oxidase (iaaod) is a novel enzyme that catalyzes the formation of isovaleraldehyde, which is the main component of mureka that gives sake an off-flavor (yamashita et al. biosci. biotechnol. biochem., 63, 1216-1222, 1999). we cloned the genomic dna sequence encoding iaaod from a koji mold, aspergillus oryzae, using a pcr-amplified dna fragment corresponding to the partial amino acid sequences of the purified protein as a probe. the cloned gene comprises 1903 bp of an open reading ... | 2000 | 16232791 |
| production and product quality assessment of human hepatitis b virus pre-s2 antigen in submerged and solid-state cultures of aspergillus oryzae. | pre-s2 is a diagnostically important antigen of human hepatitis b virus (hbv). in order to produce pre-s2 antigen in aspergillus oryzae, the gene [pre-s2]3, which encodes a tandemly triplicated repeat of pre-s2 polypeptides was fused with the partial glaa gene encoding glucoamylase lacking the starch-binding domain. in submerged culture, a. oryzae transformants carrying glaa-[pre-s2]3 secreted a heterogeneously glycosylated form of the fusion protein that was partially degraded. contrarily, util ... | 2000 | 16232829 |
| accumulation of a recombinant aspergillus oryzae lipase artificially localized on the bacillus subtilis cell surface. | cutl cdna encoding an extracellular lipase, l1, from aspergillus oryzae was fused to the cell wall-binding domain (cwb) region of a plasmid, phcb3r. sds-polyacrylamide gel electrophoresis (page) and zymography of proteins extracted from the cell surface of bacillus subtilis 168 harboring a fused lipase plasmid (phcb3rcl) revealed that the fused gene product, cwb-cutl, was localized in the b. subtilis cell wall and retained lipase activity. b. subtilis wasd (wpra sigd), recently used for the accu ... | 2000 | 16232883 |
| rapid detection of homologously integrated dna fragments and accurate quantitation of their copy number in transgenic aspergillus oryzae by pcr. | a simple and rapid method for the analysis of artificially introduced dna fragments has been developed using competitive pcr and long and accurate pcr. the locus and the copy number of the dna fragments in each aspergillus oryzae transformant could be detected more rapidly and accurately by this method than by the conventional southern hybridization method. | 2000 | 16232915 |
| molecular breeding of yeast with higher metal-adsorption capacity by expression of histidine-repeat insertion in the protein anchored to the cell wall. | a fusion protein of hexa-histidine repeat (his) and glycosylphosphatidylinositol (gpi)-anchor region of saccharomyces cerevisiae cwp1 with aspergillus oryzae taka-amylase a (taa) was expressed on the yeast cell surface. the expressed fusion protein (taa-his-cwp1) was localized on the cell wall and demonstrated amylolytic activity. in comparison with the taa-cwp1 expressing strain, these cells exhibited 1.6- to 2.8-fold higher adsorbing capacity for cu(2+), ni(2+), and zn(2+). | 2000 | 12483584 |
| the phylogenetics of mycotoxin and sclerotium production in aspergillus flavus and aspergillus oryzae. | aspergillus flavus is a common filamentous fungus that produces aflatoxins and presents a major threat to agriculture and human health. previous phylogenetic studies of a. flavus have shown that it consists of two subgroups, called groups i and ii, and morphological studies indicated that it consists of two morphological groups based on sclerotium size, called "s" and "l." the industrially important non-aflatoxin-producing fungus a. oryzae is nested within group i. three different gene regions, ... | 2000 | 11273679 |
| new syntheses of 1d- and 1l-1,2-anhydro-myo-inositol and assessment of their glycosidase inhibitory activities. | the 1d and 1l enantiomers of 1,2-anhydro-myo-inositol (conduritol b epoxide) were synthesised from 1d-pinitol and 1l-quebrachitol, respectively, and their activities were compared in selected glycosidase inhibition assays. the 1d enantiomer was found to be the active isomer, functioning as an irreversible inhibitor of sweet almond beta-d-glucosidase. neither isomer was active against the alpha-d-glucosidase from bacillus stearothermophilus or the beta-d-galactosidase from aspergillus oryzae. | 2000 | 11117313 |
| genetic analysis of growth inhibition of yeast cells caused by expression of aspergillus oryzae rnase t1. | even though most fungal hydrolytic enzymes have been successfully secreted in s. cerevisiae cells by expression of corresponding cdna, overexpression of a. oryzae rnase t1 causes severe growth inhibition in yeast. we observed that yeast strains carrying rnase t1 cdna under control of the gal1 promoter with a single-copy vector were able to grow on galactose medium while those with a multi-copy vector were not. it was found that overexpression of three mutated versions of rnase t1 with low enzyma ... | 2000 | 11129588 |
| filamentous fungus aspergillus oryzae has two types of alpha-1,2-mannosidases, one of which is a microsomal enzyme that removes a single mannose residue from man9glcnac2. | alpha-mannosidase activities towards high-mannose oligosaccharides were examined with a detergent-solubilized microsomal preparation from a filamentous fungus, aspergillus oryzae. in the enzymatic reaction, the pyridylaminated substrate man(9)glcnac(2)-pa was trimmed to man(8)glcnac(2)-pa which lacked one alpha-1,2-mannose residue at the nonreducing terminus of the middle branch (man8b isomer), and this mannooligosaccharide remained predominant through the overall reaction. trimming was optimal ... | 2000 | 11443275 |
| cloning of the aspergillus oryzae 5-aminolevulinate synthase gene and its use as a selectable marker. | the hema gene encoding 5-aminolevulinate synthase, the first enzyme in heme biosynthesis, was cloned from aspergillus oryzae and evaluated as a selectable marker for the transformation of filamentous fungi. deletion of the hema gene resulted in a lethal phenotype that could be rescued either by the supplementation of culture media with 5-aminolevulinic acid (ala) or by transformation with the wild-type hema gene, but not by growth on rich media, nor by the addition of exogenous heme. transformat ... | 2000 | 11191214 |
| molecular cloning, overexpression, and purification of a major xylanase from aspergillus oryzae. | the gene encoding xylanase g2 (xyng2) was isolated from a genomic library of aspergillus oryzae kbn616, used for making shoyu koji. the structural part of xyng2 was found to be 767 bp. the nucleotide sequence of cdna amplified by rt-pcr showed that the open reading frame of xyng2 was interrupted by a single intron which was 71 bp in size and encoded 232 amino acids. direct n-terminal amino acid sequencing showed that the precursor of xyng2 had a signal peptide of 44 amino acids. the predicted am ... | 2000 | 11210150 |
| biochemical analysis of recombinant fungal mutanases. a new family of alpha1,3-glucanases with novel carbohydrate-binding domains. | nucleotide sequence analysis shows that trichoderma harzianum and penicillium purpurogenum alpha1,3-glucanases (mutanases) have homologous primary structures (53% amino acid sequence identity), and are composed of two distinct domains: a nh(2)-terminal catalytic domain and a putative cooh-terminal polysaccharide-binding domain separated by a o-glycosylated pro-ser-thr-rich linker peptide. each mutanase was expressed in aspergillus oryzae host under the transcriptional control of a strong alpha-a ... | 2000 | 10636904 |
| myelopoietic response in tumour-bearing mice by an aggregated polymer isolated from aspergillus oryzae. | the effects of magnesium ammonium phospholinoleate-palmitoleate anhydride (mapa), a proteic aggregated polymer isolated from aspergillus oryzae, on the growth and differentiation of granulocyte-macrophage progenitor cells (colony-forming unit-granulocyte-macrophage [cfu-gm]) in normal and ehrlich ascites tumour-bearing mice were studied. myelosuppression concomitant with increased numbers of spleen cfu-gm was observed in tumour-bearing mice. treatment of these animals with mapa (0.5-10 mg/kg) st ... | 2000 | 10675729 |
| a spectrophotometric method for assay of tannase using rhodanine. | a method for assay of microbial tannase (tannin acyl hydrolase) based on the formation of chromogen between gallic acid and rhodanine is reported. unlike the previous protocols, this method is sensitive up to gallic acid concentration of 5 nmol and has a precision of 1.7% (relative standard deviation). the assay is complete in a short time, very convenient, and reproducible. | 2000 | 10683234 |
| effect of temperature and enzyme origin on the enzymatic synthesis of oligosaccharides. | the aim of this research is to quantify the effect of temperature and enzyme origin on the enzymatic synthesis of oligosaccharides. quantification of these effects is important because temperature and enzyme origin are important process parameters. a kinetic model was used to describe the concentrations in time. the kinetic parameters were determined by using data obtained in batch experiments at various temperatures (20, 30, 40, and 50 degrees c) and by using beta-galactosidases from bacillus c ... | 2000 | 10689088 |
| reversible enzyme immobilization via a very strong and nondistorting ionic adsorption on support-polyethylenimine composites. | new tailor-made anionic exchange resins have been prepared, based on films of large polyethylenimine polymers (e.g., mw 25,000) completely coating, via covalent immobilization, the surface of different porous supports (agarose, silica, polymeric resins). most proteins contained in crude extracts from different sources have been very strongly adsorbed on them. ionic exchange properties of such composites strongly depend on the size of polyethylenimine polymers as well as on the exact conditions o ... | 2000 | 10699877 |
| is fungal alpha-amylase in bread an allergen? | the enzyme alpha-amylase from aspergillus oryzae used in bakeries to improve the bread quality has been identified as an inhalative allergen in baker's asthma. it is doubtful whether this enzyme can induce allergic sensitization in regular bread consumers. | 2000 | 10718854 |
| production of extracellular lipases by penicillium cyclopium purification and characterization of a partial acylglycerol lipase. | penicillium cyclopium, grown in stationary culture, produces a type i lipase specific for triacylglycerols while, in shaken culture, it produces a type ii lipase only active on partial acylglycerols. lipase ii has been purified by ammonium sulfate precipitation and chromatographies on sephadex g-75 and deae-sephadex. the enzyme exists in several glycosylated forms of 40-43 kda, which can be converted to a single protein of 37 kda by enzymatic deglycosylation. activity of lipase ii is maximal at ... | 2000 | 10737172 |
| a simple and rapid method for the preparation of a cell-free extract with ccaat-binding activity from filamentous fungi. | a simple and rapid method for the preparation of a cell-free extract with the ccaat-binding activity was established with aspergillus nidulans as a model fungus. proteins were extracted with 6 m guanidine hydrochloride directly from mycelia and renatured by dialysis. this method was found applicable to other filamentous fungi such as aspergillus oryzae and trichoderma viride. | 2000 | 10737212 |
| efficient heterologous expression in aspergillus oryzae of a unique dye-decolorizing peroxidase, dyp, of geotrichum candidum dec 1. | efficient expression of the dye-decolorizing peroxidase, dyp, from geotrichum candidum dec 1 in aspergillus oryzae m-2-3 was achieved by fusing mature cdna encoding dyp with the a. oryzae alpha-amylase promoter (amyb). the activity yield of the purified recombinant dyp (rdyp) was 42-fold compared with that of the purified native dyp from dec 1. no exogenous heme was necessary for the expression of rdyp in a. oryzae. from the n-terminal amino acid sequence analyses of native dyp and rdyp, the abs ... | 2000 | 10742277 |
| directed deletions in the aflatoxin biosynthesis gene homolog cluster of aspergillus oryzae. | to investigate the structure of the aflatoxin gene cluster in aspergillus oryzae, 39 strains belonging to this species were examined for the existence of pksa, fas1a, aflr and vbs, and the results compared with those for ver-1 obtained previously. these five genes are involved in aflatoxin biosynthesis in aspergillus parasiticus. the strains examined were categorized into three groups; group 1, having the five homologs; 2, having ver-1 and vbs; and 3, having vbs homologs. long-pcr analysis of th ... | 2000 | 10743566 |
| evidence that the glucoamylases and alpha-amylase secreted by aspergillus niger are proteolytically processed products of a precursor enzyme. | a 125-kda starch hydrolysing enzyme of aspergillus niger characterised by its ability to dextrinise and saccharify starch [suresh et al. (1999) appl. microbiol. biotechnol. 51, 673-675] was also found to possess activity towards raw starch. segregation of these activities in the 71-kda glucoamylase and a 53-kda alpha-amylase-like enzyme supported by antibody cross-reactivity studies and the isolation of mutants based on assay screens for the secretion of particular enzyme forms revealed the 125- ... | 2000 | 10767433 |
| the influence of nitrogen sources on the alpha-amylase productivity of aspergillus oryzae in continuous cultures. | the influence of the nitrogen source on the alpha-amylase productivity of aspergillus oryzae was quantified in continuous cultivations. both inorganic and complex nitrogen sources were investigated and glucose was used as the carbon and energy sources. for production of alpha-amylase, nitrate was shown to be inferior to ammonia as a nitrogen source. a mixture of ammonia and complex nitrogen sources, such as yeast extract or casein hydrolysate, was better than with ammonia as the sole nitrogen so ... | 2000 | 10772466 |
| a novel nuclear factor, sreb, binds to a cis-acting element, sre, required for inducible expression of the aspergillus oryzae taka-amylase a gene in a. nidulans. | the taka-amylase a gene (taag2) of aspergillus oryzae is inducibly expressed in a. nidulans upon exposure to inducing carbon sources, such as starch and maltose. in order to identify nuclear factor(s) possibly involved in the induction of the taag2 gene, gel mobility shift assays and dnase i footprinting analyses were carried out, and revealed a novel nuclear factor in a. nidulans extracts, which specifically bound to two sites in the taag2 promoter region, -204 to -189 and -182 to -168, which s ... | 2000 | 10778741 |
| using dna-tagged mutagenesis to improve heterologous protein production in aspergillus oryzae. | using dna-tagged mutagenesis to improve heterologous protein production in aspergillus oryzae. fungal genetics and biology 29, 28-37. restriction enzyme-mediated integration (remi) has been employed as a mutagen to generate two insertion libraries in an aspergillus oryzae strain expressing a thermomyces lanuginosus lipase. the remi libraries were created using linearized plasmid containing the a. oryzae pyrg and either bamhi or ecori enzyme. the libraries were screened for lipase production, and ... | 2000 | 10779397 |
| molecular cloning and characterization of rpba encoding rna polymerase ii largest subunit from a filamentous fungus, aspergillus oryzae. | we have cloned rpba encoding the rna polymerase ii largest subunit (poliil) from a filamentous fungus, aspergillus oryzae. the rpba product included eight highly conserved regions and the carboxyl-terminal domain (ctd). a. oryzae poliil ctd with 184 amino acids was composed of 25 ctd consensus repeats, which was a similar number to those of lower eukaryotes. the amino acids in each repeat of a. oryzae poliil, however, conformed less to the ctd consensus than those of poliils from other lower euk ... | 2000 | 10803973 |
| agitation induced mycelial fragmentation of aspergillus oryzae and penicillium chrysogenum. | given the impact of mycelial morphology on fermentation performance, it is important to understand the factors that influence it, including agitation-induced fragmentation. the successful application of the energy dissipation/circulation function (edc) to correlate fragmentation of penicillium chrysogenum with agitation intensity and with different impeller types [5] has already been demonstrated. the edc function takes into account the specific energy dissipation rate in the impeller swept volu ... | 2000 | 10817815 |
| unique catalytic and molecular properties of hydrolases from aspergillus used in japanese bioindustries. | this review covers the unique catalytic and molecular properties of three proteolytic enzymes and a glycosidase from aspergillus. an aspartic proteinase from a. saitoi, aspergillopepsin i (ec 3.4.23.18), favors hydrophobic amino acids at p1 and p'1 like gastric pepsin. however, aspergillopepsin i accommodates a lys residue at p1, which leads to activation of trypsinogens like duodenum enteropeptidase. substitution of asp76 to ser or thr and deletion of ser78, corresponding to the mammalian aspar ... | 2000 | 10830477 |
| galactosylation of thiol group by beta-galactosidase. | beta-galactosidase catalyzed beta-galactosylation not only of a hydroxyl group but also of a thiol group in the condensation reaction of d-galactose and 2-mercaptoethanol. the thio-galactosylation product was confirmed as 2-hydroxyethyl s-beta-d-galactoside on the bases of fast atom bombardment mass spectrometry, infrared spectroscopy, and nuclear magnetic resonance spectorometry. aspergillus oryzae beta-galactosidase hydrolyzed p-nitrophenyl s-beta-d-galactoside most rapidly among several beta- ... | 2000 | 10830485 |
| molecular cloning and characterization of a transcriptional activator gene, amyr, involved in the amylolytic gene expression in aspergillus oryzae. | a gene, designated amyr, coding for a transcriptional activator involved in amylolytic gene expression has been cloned from aspergillus oryzae by screening for a clone that enabled to reverse the reduced expression of the alpha-amylase gene (amyb) promoter. amyr encodes 604 amino acid residues of a putative dna-binding protein carrying a zinc binuclear cluster motif (zn(ii)2cys6) belonging to the gal4 family of transcription factors. the amyr gene disruptants showed a significant restricted grow ... | 2000 | 10830498 |
| comprehensive cloning and expression analysis of glycolytic genes from the filamentous fungus, aspergillus oryzae. | we cloned all the glycolytic genes from aspergillus oryzae and analyzed their transcriptional regulation by the carbon source in the medium. the deduced amino-acid sequences of the glycolytic genes showed high identity (approximately 41-93%) to those from other lower eukaryotes. genomic southern hybridization indicated that all the genes existed as a single copy in the genome. comparison of mrna levels between mycelia grown on glucose and on pyruvate showed that most of the a. oryzae glycolytic ... | 2000 | 10853769 |
| a novel approach to the recovery of biologically active oligosaccharides from milk using a combination of enzymatic treatment and nanofiltration. | a new easily scalable approach to the recovery of biologically active oligosaccharides from milk has been developed which relies on the combination of enzymatic treatment of defatted milk using beta-galactosidase and nanofiltration. it was shown that enzymatic hydrolysis of lactose significantly improves the efficiency and selectivity of membrane-based separations. with the best membrane, as much as 6.7 g of oligosaccharides (containing very little contaminating lactose) could be obtained from o ... | 2000 | 10862685 |
| immobilization/stabilization on eupergit c of the beta-galactosidase from b. circulans and an alpha-galactosidase from aspergillus oryzae. | two synthetically useful glycosidases, the beta-galactosidase from bacillus circulans and an alpha-galactosidase from aspergillus oryzae have been immobilized on eupergit c. the immobilized enzymes retain high catalytic activity and show increased thermal stability compared with the free enzymes. | 2000 | 10862898 |
| [comparative characteristics of proteolytic enzyme preparations by degree of hydrolysis of a microbial protein]. | proteolytic enzymatic preparations obtained from fungi and bacteria were compared by their ability to hydrolyze yeast protein. fungal preparations were more effective. there was a more than twofold increase in the level of amine nitrogen in cell biomass hydrolysates in of cell biomass comparison to that induced by bacterial preparations. the amino acid composition of these hydrolysates was studied. amyloprotooryzin, a preparation from aspergillus oryzae 387, displayed the highest potency in prof ... | 2000 | 10867948 |
| enzymatic formation of ether linkage producing shoyuflavones from genistein and (+/-)-trans-epoxysuccinic acid. | the production mechanism of shoyuflavones, conjugated ethers of isoflavones with tartaric acid and isolated from fermented soy sauce, was studied. in the high molecular weight fraction of the culture extract of aspergillus oryzae, genistein was transformed into shoyuflavone b in the presence of (+/-)-trans-epoxysuccinic acid but not in the low molecular one. asp. sojae and asp. tamarii showed high activity similar to asp. oryzae but none of asp. niger, rhizopus oligosporus, and mucor praini did. ... | 2000 | 10888513 |
| induction of the soybean phytoalexins coumestrol and glyceollin by aspergillus. | several isoflavonoid phytoalexins produced by soybeans are known to be estrogenic, with potential beneficial health effects in humans. increased production of phytoalexins by the soybean plant will facilitate research efforts in this area. in this study, phytoalexin induction and accumulation in soybean cotyledon tissue was observed using four species of aspergillus: a. sojae, a. oryzae, a. niger, and a. flavus. all four aspergillus species tested elicited phytoalexin accumulation in living soyb ... | 2000 | 10888516 |
| noncompetitive, reversible inhibition of aminoacylase-1 by a series of l-alpha-hydroxyl and l-alpha-fluoro fatty acids: ligand specificity of aspergillus oryzae and porcine kidney enzymes. | l-lactate and l-beta-phenyllactate have been identified in the culture broth of streptomyces sp. ky-11 as reversible noncompetitive inhibitors of aspergillus oryzae aminoacylase-1 and porcine kidney aminoacylase i. a series of alpha-hydroxyl acids (dl-r-ch(oh)-cooh, r = et, n-pro, n-butyl, n-pentyl, n-hexyl) also inhibited the two enzymes in reversible noncompetitive kinetics, and the inhibition potency (-log k(i)) increased with the increased hydrophobicity of the r group. the two eukaryotic en ... | 2000 | 10898943 |