Publications

genetic diversity evaluation of some chinese elite cotton varieties with rapd markers.	rapds analysis was used to assess the genetic diversity of some elite cotton varieties. 17 cotton varieties or breeding lines were screened with 200 arbitrary primers to generate 113 polymorphic bands. cluster analysis and upgma showed that 17 varieties could be put into 3 clusters corresponding to genome difference. the genetic similarity among them ranged from 0.2000 to 0.8451. three elite varieties ejing-1, sumian-3 and zhongmiansuo-12 are highly similar in genetic base. 7581, an offspring fr ...	2000	11132498
leaf senescence-like characteristics contribute to cotton's premature photosynthetic decline.	leaf and canopy photosynthesis of cotton (gossypium hirsutum l.) declines as the crop approaches cutout, just as the assimilate needs for reproductive growth are peaking. our objective with this study was to determine whether this decline is due to remobilization of leaf components to support the reproductive growth or due to some cue from the changing environmental conditions during the growing season. field studies were conducted in 1995-1996 at stoneville, mississippi, using six cotton genoty ...	2000	16228485
effect of heat stress on the inhibition and recovery of the ribulose-1,5-bisphosphate carboxylase/oxygenase activation state.	experiments were conducted to determine the relative contributions of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco; ec 4.1.1.39) activation state vis-a-vis rubisco activase and metabolite levels to the inhibition of cotton (gossypium hirsutum l.) photosynthesis by heat stress. exposure of leaf tissue in the light to temperatures of 40 or 45 degrees c decreased the activation state of rubisco to levels that were 65 or 10%, respectively, of the 28 degrees c control. ribulose-1,5-bispho ...	2000	11219585
effect of light intensity on in vitro multiple shoot induction and regeneration of cotton (gossypium hirsutum l. cv khandawa-2).	cotyledonary nodes taken alongwith shoot apex from seedlings of cotton (g. hirsutum) proliferated into shoots on nutrient agar medium supplemented with cytokinins. in the presence of optimal plant growth regulators, low light intensity enhanced the number of shoots initiated per explant in cotton. an average of 33.5 +/- 2.9 shoots were obtained from a single explant cultured for 8 weeks which is about four fold higher than the values reported in earlier protocols. the isolated shoots were rooted ...	2000	11218821
changes in molecular species of triacylglycerols in developing cotton seeds under salt stress.	cotton plants (gossypium hirsutum l. cv. s4) were grown and irrigated with increasing salt concentrations: 0, 50, 100 and 150 mm nacl. lipids from developing seeds, at stages of 3, 4, 5 and 6 weeks after anthesis, were analysed. high salt dose (150 mm nacl) affected triacylglycerol levels drastically at the end of ripening. hplc analysis of triacylglycerols in control seeds showed ten molecular species. palmitodilinolein (pll) is the major fraction, representing about 25% of total triacylglycero ...	2000	11171251
[genetic analysis on flowering and boll setting in upland cotton (gossypium hirsutum l.). iii. genetic behavior at different developing stages].	an additive-dominant genetic model with genotype by environment interaction was employed to analyze the genetic behavior of flowering and fruiting at different development stages, with 2-year data from 4 x 4 diallel crosses. the results of variance analysis indicated that flowering and fruiting behaviors were mainly controlled by dominant effects at early period, but by additive effects later on. compared with genetic main effects, ge interaction effects playeda minor role on the flowering and f ...	2000	11132496
thermal dependence of malate synthase activity and its relationship to the thermal dependence of seedling emergence.	cotton yields are often reduced by low temperatures at early planting dates. improved seedling metabolism at low temperatures may enhance seedling performance. the glyoxylate cycle plays a role in the metabolism of stored lipids, and thus thermal limitations on the function of malate synthase (ec 4.1.3.2) may be involved in low-temperature limitations on seedlings. the thermal dependencies of the apparent k(m) and maximal velocity for the malate synthases from cotton (gossypium hirsutum l.) and ...	2000	11052697
effects of mild night chilling on respiration of expanding cotton leaves.	utilizing expanding leaves of cotton (gossypium hirsutum l. cv. coker 312), the hypothesis that suboptimal night temperatures above those for putative phase transitions of mitochondrial lipids caused greater substrate control of night respiration and increased the control that respiration exerted on atp-dependent metabolism was tested. night respiratory co(2) evolution rates for 5-7-day-old leaves growing at 30/19 degrees (day/night) nearly equaled those of leaves exposed to 28 degrees c nights, ...	2000	10960737
multilocus interactions restrict gene introgression in interspecific populations of polyploid gossypium (cotton).	experimental advanced-generation backcross populations contain individuals with genomic compositions similar to those resulting from interspecific hybridization in nature. by applying a detailed restriction fragment length polymorphism (rflp) map to 3662 bc3f2 plants derived from 24 different bc1 individuals of a cross between gossypium hirsutum and g. barbadense, large and widespread deficiencies of donor (g. barbadense) chromatin were found, and seven independent chromosomal regions were entir ...	2000	10937254
ty1-copia-retrotransposon behavior in a polyploid cotton.	retrotransposons constitute a ubiquitous and dynamic component of plant genomes. intragenomic and intergenomic comparisons of related genomes offer potential insights into retrotransposon behavior and genomic effects. here, we have used fluorescent in-situ hybridization to determine the chromosomal distributions of a ty1-copia-like retrotransposon in the cotton ad-genome tetraploid gossypium hirsutum and closely related putative a- and d-genome diploid ancestors. retrotransposon clone a108 hybri ...	2000	10730591
dynamics of non-structural carbohydrates in developing leaves, bracts and floral buds of cotton.	development of cotton (gossypium hirsutum l.) squares (i.e. floral buds with bracts) is fundamental for yield formation. a 2-year field study was conducted to determine dry weight (dw) accumulations of cotton leaves, floral bracts and floral buds, and the changes in concentrations of non-structural carbohydrates (hexoses, sucrose and starch) in these tissues during square ontogeny as affected by fruiting positions within the plant canopy. during square development, dw accumulation of a subtendin ...	2000	10725518
an assessment of alphaalpha-tubulin isotype modification in developing cotton fiber.	multiple isoforms of alpha- and beta-tubulin accumulate in higher plant cells, including cotton (gossypium hirsutum l.) fiber. isotypes may originate either by transcription of distinct genes or by posttranslational modification of gene products. the existence of two types of posttranslational modification in cotton fiber alpha-tubulin has been examined by immunoblotting cotton fiber proteins from two developmental stages and by probing with specific monoclonal antibodies to acetylated (6-11b-1) ...	2000	10648195
expression of a lipid transfer protein gene family during cotton fibre development.	there are six to eight lipid transfer protein-related genes in the allotetraploid gossypium hirsutum genome, three of which, fsltp1, fsltp2 and fsltp3, were isolated in two genomic clones. all three genes potentially encode full-length lipid transfer proteins (ltps), but sequence differences between these and the cotton fibre-specific cdna previously reported, pfs6, suggest that none is likely to encode the fibre transcripts. gene-specific rt-pcr experiments showed that expression patterns of th ...	2000	10634943
identification and cloning of a complementary dna encoding a vicilin-like proprotein, jug r 2, from english walnut kernel (juglans regia), a major food allergen.	walnuts and other tree nuts are important food-allergen sources that have the potential to be associated with life-threatening, ige-mediated systemic reactions in some individuals.	1999	10589017
duplicated genes evolve independently after polyploid formation in cotton.	of the many processes that generate gene duplications, polyploidy is unique in that entire genomes are duplicated. this process has been important in the evolution of many eukaryotic groups, and it occurs with high frequency in plants. recent evidence suggests that polyploidization may be accompanied by rapid genomic changes, but the evolutionary fate of discrete loci recently doubled by polyploidy (homoeologues) has not been studied. here we use locus-specific isolation techniques with comparat ...	1999	10588718
conditioning of fasciation by gibberellin and genotype in cotton (gossypium hirsutum l.).	the round-leafed mutant cotton line l-501 developed fasciation of the upper stem when field grown in central asia. fasciation co-segregated with the mutant gene for round leaves in.(l) fasciation developed at the flowering stage, but removal of floral buds did not prevent fasciation. fasciation in l-501 could be inhibited by the gibberellin (ga) biosynthesis inhibitor chlorocholine chloride or by fusicoccin. ga(3) application in the field induced fasciation in the mutant's parental line l-463, w ...	1999	10467019
mobility of the herbicide pyrithiobac through intact soil columns.	the relative mobility of pyrithiobac [sodium 2-chloro-6-(4, 6-dimethoxypyrimidin-2-ylthio)benzoate], a new herbicide used for postemergence control of broadleaf weeds in cotton (gossypium hirsutum), was evaluated and compared against that of bromide (br(-)) tracer on four soils representative of cotton-growing regions using intact soil columns under saturated flow conditions. pyrithiobac breakthrough curves were asymmetrical in shape with significant tailing and displaced to the left of 1 pore v ...	1999	10564050
hmg-coa reductase gene family in cotton (gossypium hirsutum l.): unique structural features and differential expression of hmg2 potentially associated with synthesis of specific isoprenoids in developing embryos.	as a first step towards understanding the biosynthesis of isoprenoids that accumulate in specialized pigment glands of cotton at the molecular level, two full-length genes (hmg1 and hmg2) were characterized encoding hmg-coa reductase (hmgr; ec 1.1.1.34), the enzyme that catalyzes the formation of a key isoprenoid precursor. cotton hmgr genes exhibited features typical of other plant genes, however, hmg2 encodes the largest of all plant hmgr enzymes described to date. hmg2 contains several novel ...	1999	10501034
gene-specific changes in alpha-tubulin transcript accumulation in developing cotton fibers.	the fibers of cotton (gossypium hirsutum) are single-cell trichomes that undergo rapid and synchronous elongation. cortical microtubules provide spatial information necessary for the alignment of cellulose microfibrils that confine and regulate cell elongation. we used gene-specific probes to investigate alpha-tubulin transcript levels in elongating cotton fibers. two discrete patterns of transcript accumulation were observed. whereas transcripts of alpha-tubulin genes ghtua2/3 and ghtua4 increa ...	1999	10482673
new ribosomal rna gene locations in gossypium hirsutum mapped by meiotic fish.	in this study we have mapped newly identified rdna loci in gossypium hirsutum. four new minor 18s-26s rdna loci, in addition to the sites previously identified, were mapped using fluorescence in situ hybridization (fish) to heterozygous translocation (nt) quadrivalents (ivs). the newly detected 18s-26s rdna loci were mapped to the right arms of chromosomes 8, 9, 15, 17, 19, 20, and 23 and the left arms of chromosomes 5, 11, 12, and 14. using the rdna loci as common reference points, we detected ...	1999	10398849
differential regulation of six novel myb-domain genes defines two distinct expression patterns in allotetraploid cotton (gossypium hirsutum l.).	a pcr-based strategy was employed to identify myb-related genes potentially involved in the differentiation and development of cotton seed trichomes. cdna clones representing six newly identified cotton myb-domain genes (ghmyb) of the r2r3-myb family were characterized in the allotetraploid species gossypium hirsutum l. (2n = 4x = 52; aadd). several interesting motifs and domains in the transregulatory region (trr) were identified as potential candidates for modulating ghmyb activity. one such s ...	1999	10394903
low levels of nucleotide diversity at homoeologous adh loci in allotetraploid cotton (gossypium l.).	levels of genetic diversity within and among populations and species are shaped by both external (population-level) and internal (genomic and genic) evolutionary forces. to address the effect of internal pressures, we estimated nucleotide diversity for a pair of homoeologous adh loci in an allotetraploid species, gossypium hirsutum. these data represent the first such estimates for a pair of homoeologous nuclear loci in plants. estimates of nucleotide diversity for adha in gossypium are lower th ...	1999	10331275
inhibition and acclimation of photosynthesis to heat stress is closely correlated with activation of ribulose-1,5-bisphosphate carboxylase/oxygenase	increasing the leaf temperature of intact cotton (gossypium hirsutum l.) and wheat (triticum aestivum l.) plants caused a progressive decline in the light-saturated co2-exchange rate (cer). cer was more sensitive to increased leaf temperature in wheat than in cotton, and both species demonstrated photosynthetic acclimation when leaf temperature was increased gradually. inhibition of cer was not a consequence of stomatal closure, as indicated by a positive relationship between leaf temperature an ...	1999	10318695
coordinated accumulation of (+)-delta-cadinene synthase mrnas and gossypol in developing seeds of gossypium hirsutum and a new member of the cad1 family from g. arboreum.	a new member of the (+)-delta-cadinene synthase (cad1) family was isolated from a gossypium arboreum cdna library. this cdna encodes a protein that showed 97.3%, 96.9%, and 79.2% sequence identities with the proteins encoded by previously isolated cdnas of cad1-c1, cad1-c14, and cad1-a, respectively. it may be grouped into the cad1-c subfamily as cad1-c2. seeds of a glanded cotton cultivar, g. hirsutum cv. sumian-6, were collected at different intervals during maturation, and the cad1 mrna level ...	1999	10075752
glycerol is a suberin monomer. new experimental evidence for an old hypothesis	the monomer composition of the esterified part of suberin can be determined using gas chromatography-mass spectroscopy technology and is accordingly believed to be well known. however, evidence was presented recently indicating that the suberin of green cotton (gossypium hirsutum cv green lint) fibers contains substantial amounts of esterified glycerol. this observation is confirmed in the present report by a sodium dodecyl sulfate extraction of membrane lipids and by a developmental study, demo ...	1999	10069853
heterologous myb genes distinct from gl1 enhance trichome production when overexpressed in nicotiana tabacum.	myb-class transcription factors implicated in cell shape regulation were overexpressed in arabidopsis thaliana and nicotiana tabacum in an attempt to assess the extent to which cellular differentiation programs might be shared between these distantly related plants. glabrous 1, a myb gene required for trichome development in arabidopsis, did not alter the trichome phenotype of the tobacco plants in which it was overexpressed. mixta, which in antirrhinum majus is reported to regulate certain aspe ...	1999	9895315
gtpase activity and biochemical characterization of a recombinant cotton fiber annexin.	a cdna encoding annexin was isolated from a cotton (gossypium hirsutum) fiber cdna library. the cdna was expressed in escherichia coli, and the resultant recombinant protein was purified. we then investigated some biochemical properties of the recombinant annexin based on the current understanding of plant annexins. an "add-back experiment" was performed to study the effect of the recombinant annexin on beta-glucan synthase activity, but no effect was found. however, it was found that the recomb ...	1999	10069831
increased nuclear dna content in developing cotton fiber cells.	the nuclear dna content of developing cotton fiber cells (gossypium hirsutum, cv. md51ne) increases ~24% after 2 d postanthesis (dpa). the amount of nuclear dna at 2 dpa is 5.4 +/- 0.27 pg. at 3-4 dpa it increases to 6.7 +/- 0.24 pg and by 5 dpa it is 6.8 +/- 0.70 pg. these values were obtained by nuclear fluorescence after staining with hoechst 33258. human oral squamous cell nuclei were used as a dna standard. nuclear dna content increases in fibers growing on either fertilized or unfertilized ...	1999	10371719
identification of disease response genes expressed in gossypium hirsutum upon infection with the wilt pathogen verticillium dahliae.	verticillium wilt is a vascular disease of cotton (gossypium spp.) caused by the fungal pathogen verticillium dahliae. to begin to understand the molecular mechanisms of the disease response in cotton cultivars that display superior wilt tolerance, such as gossypium hirsutum cv. sicala v-1, a cdna library was constructed with mrna isolated from root tissue of sicala v-1, 24 h after inoculation with v. dahliae. the library was screened by a differential screening technique which was successful in ...	1999	10412907
interplant movement of heliothis virescens (lepidoptera: noctuidae) larvae in pure and mixed plantings of cotton with and without expression of the cry1ac delta-endotoxin protein of bacillus thuringiensis berliner.	laboratory and field studies were conducted during 1993 and 1994 to quantify interplant movement of heliothis virescens (f.) larvae in pure and mixed plantings of cotton, gossypium hirsutum l., with ('event 531') and without ('coker 312') the expression of cry1ac delta-endotoxin protein of bacillus thuringiensis berliner. field studies were conducted with neonate, 4-, and 7-d-old larvae placed on 3-plant experimental units and observed at 24, 48, 72, and 96 h after inoculation of larvae. combini ...	1999	10504897
identification and expression of cotton (gossypium hirsutum l.) plastidial carbonic anhydrase.	four carbonic anhydrase (ca) cdna clones were isolated from a 48 h dark-grown cotton (gossypium hirsutum l.) seedling cdna library. nucleotide sequence analysis revealed two different ca isoforms designated ghca1 and ghca2. the encoded polypeptides possess n-terminal serine/threonine-rich regions indicative of plastid transit peptides, and approximately 80% sequence identity to other plant plastidial beta-cas. the ghca1 cdna encodes a nearly complete preprotein of 323 amino acids with a molecula ...	1999	10682348
survey of crop losses in response to phytoparasitic nematodes in the united states for 1994.	previous reports of crop losses to plant-parasitic nematodes have relied on published results of survey data based on certain commodities, including tobacco, peanuts, cotton, and soybean. reports on crop-loss assessment by land-grant universities and many commodity groups generally are no longer available, with the exception of the university of georgia, the beltwide cotton conference, and selected groups concerned with soybean. the society of nematologists extension committee contacted extensio ...	1999	19270925
comparison of variable and single-rate applications of aldicarb on cotton yield in fields infested with meloidogyne incognita.	variable-rate applications of the nematicide aldicarb were compared to producer standard rates in eight field tests over 3 years. test areas (308 to 1,015 m long) were divided into eight or five blocks. each block contained two plots with a variable-rate treatment (vrt) of aldicarb and a producer standard treatment (pst) of aldicarb. each vrt plot was divided into three subunits and intensively sampled for meloidogyne incognita in either the fall or spring before planting. rates of aldicarb were ...	1999	19270939
bahiagrass, corn, cotton rotations, and pesticides for managing nematodes, diseases, and insects on peanut.	florunner peanut was grown after 1 and 2 years of tifton 9 bahiagrass, corn, cotton, and continuous peanut as whole-plots. pesticide treatments aldicarb (3.4 kg a.i./ha), flutolanil (1.7 kg a.i./ha), aldicarb + flutolanil, and untreated (control) were sub-plots. numbers of meloidogyne arenaria second-stage juveniles in the soil and root-gall indices of peanut at harvest were consistently lower in plots treated with aldicarb and aldicarb + flutolanil than in flutolanil-treated and untreated plots ...	1999	19270889
the involvement of hydrogen peroxide in the differentiation of secondary walls in cotton fibers	h2o2 is a widespread molecule in many biological systems. it is created enzymatically in living cells during various oxidation reactions and by leakage of electrons from the electron transport chains. depending on the concentration h2o2 can induce cell protective responses, programmed cell death, or necrosis. here we provide evidence that h2o2 may function as a developmental signal in the differentiation of secondary walls in cotton (gossypium hirsutum) fibers. three lines of evidence support th ...	1999	10069824
responses of cotton yield and meloidogyne incognita soil populations to soil applications of aldicarb and 1,3-d in florida.	in four tests conducted in loamy-sand soils in northern florida, cotton lint yield increased and post-harvest soil populations of meloidogyne incognita were more effectively suppressed by 1,3-dichloropropene (1,3-d) than aldicarb when both nematicides were evaluated over a range of recommended rates. significant positive relationships existed between lint yield and rates of 1,3-d in three tests, whereas only one significant positive relationship occurred between lint yield and aldicarb rates. yi ...	1998	19274259
interaction between meloidogyne incognita and thielaviopsis basicola on cotton (gossypium hirsutum).	the effects of meloidogyne incognita and thielaviopsis basicola on the growth of cotton (gossypium hirsutum) and the effects of t. basicola on m. incognita populations were evaluated in a 2-year study. microplots were infested with m. incognita, t. basicola, or a combination of m. incognita and t. basicola. uninfested plots served as controls both years. seedling survival was decreased by the m. incognita + t. basicola treatment compared to the control. meloidogyne incognita alone and m. incogni ...	1998	19274234
peanut-cotton-rye rotations and soil chemical treatment for managing nematodes and thrips.	in the southeastern united states, a cotton-peanut rotation is attractive because of the high value and extensive planting of both crops in the region. the objective of this experiment was to determine the effects of cotton-peanut rotations, rye, and soil chemical treatments on management of plant-parasitic nematodes, thrips, and soilborne fungal diseases and on crop yield. peanut-cotton-rye rotations were conducted from 1988 to 1994 on tifton loamy sand (plinthic kandiudult) infested primarily ...	1998	19274213
crop yields and nematode population densities in triticale-cotton and triticale-soybean rotations.	triticale cv. beagle 82, cotton cv. mcnair 235, and soybean cv. twiggs were arranged in three cropping sequences to determine the effects of fenamiphos and cropping sequence on nematode population densities and crop yields under conservation tillage for 4 years. the cropping sequences were triticale (t)-cotton (c)-t-c, t-soybean (s)-t-s, and t-c-t-s. numbers of meloidogyne incognita second-stage juveniles declined on trificale but increased on cotton and soybean each year. root-gall indices of c ...	1998	19274228
manipulation of catalase levels produces altered photosynthesis in transgenic tobacco plants.	constructs containing the cdnas encoding the primary leaf catalase in nicotiana or subunit 1 of cottonseed (gossypium hirsutum) catalase were introduced in the sense and antisense orientation into the nicotiana tabacum genome. the n. tabacum leaf cdna specifically overexpressed cat-1, the high catalatic [corrected] form, activity. antisense constructs reduced leaf catalase specific activities from 0.20 to 0.75 times those of wild type (wt), and overexpression constructs increased catalase specif ...	1998	9449845
analysis of plastid dna-like sequences within the nuclear genomes of higher plants.	a wide-ranging examination of plastid (pt)dna sequence homologies within higher plant nuclear genomes (promiscuous dna) was undertaken. digestion with methylation-sensitive restriction enzymes and southern analysis was used to distinguish plastid and nuclear dna in order to assess the extent of variability of promiscuous sequences within and between plant species. some species, such as gossypium hirsutum (cotton), nicotiana tabacum (tobacco), and chenopodium quinoa, showed homogenity of these se ...	1998	9615455
polyploid formation created unique avenues for response to selection in gossypium (cotton).	a detailed restriction fragment length polymorphism map was used to determine the chromosomal locations and subgenomic distributions of quantitative trait loci (qtls) segregating in a cross between cultivars of allotetraploid (aadd) gossypium hirsutum ("upland" cotton) and gossypium barbadense ("sea island," "pima," or "egyptian" cotton) that differ markedly in the quality and quantity of seed epidermal fibers. most qtls influencing fiber quality and yield are located on the "d" subgenome, deriv ...	1998	9539752
isolation of a cotton cap gene: a homologue of adenylyl cyclase-associated protein highly expressed during fiber elongation.	the cdna encoding cap (adenylyl cyclase-associated protein) was isolated from a cotton (gossypium hirsutum) fiber cdna library. the cdna (ghcap) contained an open reading frame that encoded 471 amino acid residues. rna blot analysis showed that the cotton cap gene was expressed mainly in young fibers.	1998	10050322
the lance nematode, hoplolaimus magnistylus, on cotton in arkansas.	the population density of hoplolaimus magnistylus, a lance nematode, in cotton was determined at planting, mid-season, and harvest during the 1995 and 1996 growing seasons for a poinsett county, arkansas field. nematode populations increased from planting to harvest in 1995 but declined in 1996. application of aldicarb at planting at rates of 0.59 or 0.84 kg a.i./ha did not influence either nematode population density or cotton yield. this study indicates that h. magnistylus is not a serious pes ...	1998	19274251
structural analysis of a hmg-coa-reductase pseudogene: insights into evolutionary processes affecting the hmgr gene family in allotetraploid cotton (gossypium hirsutum l.).	structural analysis of hmg-coa reductase (hmgr) genes in the allotetraploid cotton species gossypium hirsutum l. revealed the first-known existence of a pseudogene, psihmg5, for this important enzyme. complete sequencing of the genomic clone hmg5 unveiled several deleterious lesions, resulting in an organization that departed significantly from the linear canonical hmgr gene structure. although analysis of the 5' flanking region indicated a promoter-like composition based on comparison with othe ...	1998	9799357
a fiberless seed mutation in cotton is associated with lack of fiber cell initiation in ovule epidermis and alterations in sucrose synthase expression and carbon partitioning in developing seeds	fiber cell initiation in the epidermal cells of cotton (gossypium hirsutum l.) ovules represents a unique example of trichome development in higher plants. little is known about the molecular and metabolic mechanisms controlling this process. here we report a comparative analysis of a fiberless seed (fls) mutant (lacking fibers) and a normal (fls) mutant to better understand the initial cytological events in fiber development and to analyze the metabolic changes that are associated with the loss ...	1998	9765525
genes involved in osmoregulation during turgor-driven cell expansion of developing cotton fibers are differentially regulated.	cotton (gossypium hirsutum l.) fibers are single-celled trichomes that synchronously undergo a phase of rapid cell expansion, then a phase including secondary cell wall deposition, and finally maturation. to determine if there is coordinated regulation of gene expression during fiber expansion, we analyzed the expression of components involved in turgor regulation and a cytoskeletal protein by measuring levels of mrna and protein accumulation and enzyme activity. fragments of the genes for the p ...	1998	9536073
moderately high temperatures inhibit ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) activase-mediated activation of rubisco	we tested the hypothesis that light activation of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) is inhibited by moderately elevated temperature through an effect on rubisco activase. when cotton (gossypium hirsutum l.) or wheat (triticum aestivum l.) leaf tissue was exposed to increasing temperatures in the light, activation of rubisco was inhibited above 35 and 30 degreesc, respectively, and the relative inhibition was greater for wheat than for cotton. the temperature-induced inhib ...	1998	9490757
growth and mitotic potential of multicelled fibers of cotton (malvaceae).	we tested the hypothesis that the growth of multicelled cotton fibers of gossypium hirsutum, cultivar md51 ne, occurs exclusively within the tip cell. direct cellular measurements proved the hypothesis incorrect. the results show that all cells within a fiber grow and that the relative growth of the tip cell is reduced as the number of cells per fiber increases. also, measurements of two- and three-celled fibers show that the two daughter nuclei in two-celled fibers differ. the ability to divide ...	1998	21684876
cotton fibers can undergo cell division.	ovular culture was used to determine the cell cycle aspects of cotton fiber cells. each ovule (gossypium hirsutum, cultivar, md51 ne) grown under the conditions used has ~10 000 fiber cells at 4 d postanthesis. about 25% of these cells divide when ovules are cultured at 34c. mitosis occurs after fiber cells differentiate, producing multicelled fibers. the basal and tip cells of multicelled fibers have the same characteristics as the polar ends of single-celled fibers. most cell division occurs i ...	1997	21708678
identification of a delta-tip cdna clone and determination of related a and d genome subfamilies in gossypium species.	tonoplast intrinsic proteins (tips) have been implicated in the process of cell elongation, such as occurs in the developing cotton fiber. we have isolated a cdna clone (997 bp in length) from a cotton (gossypium hirsutum l.) library which putatively encodes a protein of 248 residues (mr 25079) with 85% identity to arabidopsis delta-tip. the derived amino acid sequence included two conserved sequences associated with major intrinsic proteins (sgxhxnpa at residues 78 to 85, npa residues at 197 to ...	1997	9177317
identification of a cotton fiber-specific acyl carrier protein cdna by differential display.	transcripts from immature fibers and stripped ovules (fibers removed) of cotton (gossypium hirsutum l.) were compared by differential display to identify cdna fragments that represent mrnas that are expressed primarily in cotton fibers. eight independent fiber-specific cdna fragments were isolated. one of these cdnas had strong sequence similarity with acyl carrier protein (acp). a full-length cdna for the cotton fiber-specific acp was isolated using a pcr cdna library screening technique. this ...	1997	9130594
interference among cotton neighbours after differential reproductive damage.	in indeterminate plant species, the rate of vegetative growth usually declines during the stage of active reproductive growth. fruit shedding, as induced by insect herbivores, could counteract this decline. due to the relative increase in vegetative growth, plants that have suffered reproductive damage could be better able to intercept light and acquire soil resources than undamaged plants. if so, plants with damaged neighbours might grow less than their counterparts with smaller, undamaged neig ...	1997	28307540
genetic diversity evaluation of some elite cotton varieties by rapd analysis.	random amplified polymorphic dna (rapd) analysis was used to evaluate the genetic diversity of elite commercial cotton varieties. twenty two varieties belonging to gossypium hirsutum l. and one to g. arboreum l. were analyzed with 50 random decamer primers using the polymerase chain reaction (pcr). forty nine primers detected polymorphism in all 23 cotton varieties, while one produced monomorphic amplification profiles. a total of 349 bands were amplified, 89.1% of which were polymorphic. cluste ...	1997	19352756
the differential expression of sucrose synthase in relation to diverse patterns of carbon partitioning in developing cotton seed.	developing cotton (gossypium hirsutum l.) seed exhibits complex patterns of carbon allocation in which incoming sucrose (suc) is partitioned to three major sinks: the fibers, seed coat, and cotyledons, which synthesize cellulose, starch, and storage proteins or oils, respectively. in this study we investigated the role of suc synthase (susy) in the mobilization of suc into such sinks. assessments of susy gene expression at various levels led to the surprising conclusion that, in contrast to that ...	1997	12223814
quantum yields and rate constants of photochemical and nonphotochemical excitation quenching (experiment and model).	sunflower (helianthus annuus l.), cotton (gossypium hirsutum l.), tobacco (nicotiana tabacum l.), sorghum (sorghum bicolor moench.), amaranth (amaranthus cruentus l.), and cytochrome b6f complex-deficient transgenic tobacco leaves were used to test the response of plants exposed to differnt light intensities and co2 concentrations before and after photoinhibition at 4000 [mu]mol photons m-2 s-1 and to thermoinhibition up to 45[deg]c. quantum yields of photochemical and nonphotochemical excitatio ...	1997	12223845
use of meiotic fish for identification of a new monosome in gossypium hirsutum l.	the extensive use of molecular cytogenetics in human genetics and clinical diagnostics indicates that analogous applications in plants are highly feasible. one sort of application would be the identification of new aneuploids, which traditionally involves either direct karyotypic identification, which is feasible in only a few plant species, or tests with markers (cytogenetic, genetic, or molecular), which require sexual hybridization and at least one subsequent seed or plant generation. we have ...	1997	9061912
resistance to meloidogyne incognita race 3 and rotylenchulus reniformis in wild accessions of gossypium hirsutum and g. barbadense from mexico.	forty-six accessions of g. hirsutum and two of g. barbadense were examined for resistance to meloidogyne incognita race 3 and rotylenchulus reniformis in environmental growth chamber experiments, with the objective of finding new sources of resistance. only the g. barbadense accessions, tx-1347 and tx-1348, supported significantly less reproduction by r. reniformis than the susceptible control, deltapine 16 (usda accession sa-1186). however, they were highly susceptible to m. incognita race 3. t ...	1997	19274280
tolerance to rotylenchulus reniformis and resistance to meloidogyne incognita race 3 in high-yielding breeding lines of upland cotton.	field experiments in 1992 and 1994 were conducted to determine the effect of rotylenchulus reniformis, reniform nematode, on lint yield and fiber quality of 10 experimental breeding lines of cotton (gossypium hirsutum) in untreated plots or plots fumigated with 1,3-dichloropropene. controls were la. rn 1032, a germplasm line possessing some resistance to r. reniformis, and stoneville 453, a cultivar that is susceptible to reniform nematode. several breeding lines produced greater lint yields tha ...	1997	19274165
inoculum densities of fusarium oxysporum f. sp. vasinfectum and meloidogyne incognita in relation to the development of fusarium wilt and the phenology of cotton plants (gossypium hirsutum).	abstract development of fusarium wilt in upland cotton (gossypium hirsutum) usually requires infections of plants by both meloidogyne incognita and fusarium oxysporum f. sp. vasinfectum. in this study, the soil densities of m. incognita and f. oxysporum f. sp. vasinfectum and the incidence of fusarium wilt in three field sites were determined in 1982-1984. multiple regression analysis of percent incidence of fusarium wilt symptoms on population densities of m. incognita and f. oxysporum f. sp. v ...	1997	18945178
evaluation of nemx, a new cultivar of cotton with high resistance to meloidogyne incognita.	the level of resistance to root-knot nematode, meloidogyne incognita, in nemx, a new cultivar of the acala-type upland cotton, was evaluated in relation to four resistant breeding lines (n6072, n8577, n901, and n903) and four susceptible cultivars (maxxa, sj2, royale, and prema). in growth pouch tests, an average of only 4 nematode egg masses was produced on roots of nemx or the resistant lines, compared to a significantly higher average of 21 on the susceptible cultivars. in pot tests, the nema ...	1997	19274190
de novo biosynthesis of volatiles induced by insect herbivory in cotton plants.	in response to insect feeding on the leaves, cotton (gossypium hirsutum l.) plants release elevated levels of volatiles, which can serve as a chemical signal that attracts natural enemies of the herbivore to the damaged plant. pulse-labeling experiments with [13c]co2 demonstrated that many of the volatiles released, including the acyclic terpenes (e,e)-[alpha]-farnesene, (e)-[beta]-farnesene, (e)-[beta]-ocimene, linalool, (e)-4,8-dimethyl-1,3,7-nonatriene, and (e/e)-4,8,12-trimethyl-1,3,7,11-tri ...	1997	12223763
sequence and rt-pcr expression analysis of two peroxidases from arabidopsis thaliana belonging to a novel evolutionary branch of plant peroxidases.	cdna clones encoding two new arabidopsis thaliana peroxidases, atp 1a and atp 2a, have been identified by searching the arabidopsis database of expressed sequence tags (dbest). they represent a novel branch of hitherto uncharacterized plant peroxidases which is only 35% identical in amino acid sequence to the well characterized group of basic plant peroxidases represented by the horseradish (armoracia rusticana) isoperoxidases hrp c, hrp e5 and the similar arabidopsis isoperoxidases atp ca, atp ...	1997	9132061
meloidogyne incognita infested soil amended with chicken litter.	the effects of chicken litter on meloidogyne incognita in cotton, gossypium hirsutum cv. dpl50 were determined in field microplots. litters (manure and pine-shaving bedding) from a research facility and a commercial broiler house were used. treatments consisted of 0.25%, 0.5%, and 1% litter by dry weight of soil for each kind of litter. three control treatments consisted of soil not amended with litter, with and without nematodes, and one treatment to which mineral fertilizer was added at a nitr ...	1996	19277155
impact of soil texture on the reproductive and damage potentials of rotylenchulus reniformis and meloidogyne incognita on cotton.	the effects of soil type and initial inoculum density (pi) on the reproductive and damage potentials of meloidogyne incognita and rotylenchulus reniformis on cotton were evaluated in microplot experiments from 1991 to 1993. the equilibrium nematode population density for r. reniformis on cotton was much greater than that of m. incognita, indicating that cotton is a better host for r. reniformis than m. incognita. reproduction of m. incognita was greater in coarse-textured soils than in fine-text ...	1996	19277171
in vitro prenylation of the small gtpase rac13 of cotton.	previous work (d.p. delmer, j. pear, a. andrawis, d. stalker [1995] mol gen genet 248: 43-51) has identified a gene in cotton (gossypium hirsutum), rac13, that encodes a small, signal-transducing gtpase and shows high expression in the fiber at the time of transition from primary to secondary wall synthesis. since rac13 may be important in signal transduction pathway(s), regulating the onset of fiber secondary wall synthesis, we continue to characterize rac13 by determining its ability to underg ...	1996	12226460
purification of (+)-delta-cadinene synthase, a sesquiterpene cyclase from bacteria-inoculated cotton foliar tissue.	a sesquiterpene cyclase whose activity is induced in a glandless, bacterial blight-resistant line of cotton (gossypium hirsutum l.) catalyses the conversion of (e,e)-farnesyl diphosphate to (+)-delta-cadinene. this enzyme was purified by a combination of salt-induced phase separation, hydroxylapatite fractionation, hydrophobic interaction and strong anion-exchange chromatography, and denaturing polyacrylamide gel electrophoresis, followed by renaturation with tween 80. the purified enzyme has a ...	1996	8728715
higher plants contain homologs of the bacterial cela genes encoding the catalytic subunit of cellulose synthase.	in spite of much effort, no one has succeeded in isolating and characterizing the enzyme(s) responsible for synthesis of cellulose, the major cell wall polymer of plants. we have characterized two cotton (gossypium hirsutum) cdna clones and identified one rice (oryza sativa) cdna that are homologs of the bacterial cela genes that encode the catalytic subunit of cellulose synthase. three regions in the deduced amino acid sequences of the plant cela gene products are conserved with respect to the ...	1996	8901635
characterization and expression of chitinase and 1,3-beta-glucanase genes in cotton.	we have isolated cdna clones representing mrnas encoding chitinase and 1,3-beta-glucanase in cotton (gossypium hirsutum l.) leaves. the chitinase clones were sequenced and found to encode a 28,806 da protein with 71% amino acid sequence similarity to the sk2 chitinase from potato (solanum tuberosum). the 1,3-beta-glucanase clones encoded a 37,645 da protein with 57.6% identity to a 1,3-beta-glucanase from soybean (glycine max). northern blot analyses showed that chitinase mrna is induced in plan ...	1996	8806421
frequency and geographical distribution of plant-parasitic nematodes on cotton in georgia.	a survey was conducted to examine the geographical distribution of plant-parasitic nematodes in georgia cotton fields. a total of 778 fields in 11 georgia counties were sampled from 1 september through 15 december 1995. four nematode genera parasitic on cotton were found in this survey: meloidogyne spp., rotylenchulus sp., hoplolaimus sp., and belonolaimus sp. meloidogyne spp. was present in 9% to 56% of the fields in individual counties. rotylenchulus sp. was found in 10 counties, hoplolaimus s ...	1996	19277192
antioxidant response to nacl stress in a control and an nacl-tolerant cotton cell line grown in the presence of paraquat, buthionine sulfoximine, and exogenous glutathione.	a cotton (gossypium hirsutum l.) control and nacl-tolerant cell line (cv coker 312) were grown on media with or without nacl in the presence or absence of paraquat, buthionine sulfoximine, and oxidized glutathione. on medium with 150 mm nacl the nacl-tolerant cell line exhibited no reduction in growth, whereas a 96% reduction was observed in the control line. the nacl-tolerant cell line that was grown on 150 mm nacl exhibited significantly greater catalase (341%), peroxidase (319%), glutathione ...	1996	12226422
ribosomal protein rl44 is encoded by two subfamilies in upland cotton (gossypium hirsutum l.).	we have isolated 4 cdna clones encoding the full-length sequence of the eukaryotic ribosomal protein rl44 from upland cotton (gossypium hirsutum l.). sequencing of these clones resulted in the classification of 2 subfamilies of rl44; these subfamilies had coding regions (315 bp) which were 92% identical. rl44-1 (454 bp) and rl44-2 (485 bp) constitute subfamily 1, whereas rl44-3 (913 bp) and rl44-5 (541 bp) constitute subfamily 2. the differences in nucleotide sequences, however, occurred only at ...	1996	8806588
regeneration of plants from cryopreserved embryogenic cell suspension and callus cultures of cotton (gossypium hirsutum l.).	successful regeneration of cotton (gossypium hirsutum l.) plants from cryopreserved embryogenic callus and cell suspension cultures is described. the cryoprotectant mixture consisting of a modified murashige and skoog (1962) medium with sucrose (5% w/v), dmso (5% v/v) and glycerol (5% v/v) gave the highest survival rate (70%) from cell suspension cultures cryopreserved in liquid nitrogen after slow cooling (0.5 to 1.0°c/min). a cooling rate of 0.5°c/min provided a satisfactory recovery rate (30% ...	1996	24178225
ish-facilitated analysis of meiotic bivalent pairing.	chiasmata constitute one of the cornerstones of sexual reproduction in most eukaryotes. they mediate the reciprocal genetic exchange between homologues and are essential to the proper orientation of the homologous centromeres in meiosis i. as markers of recombination, they offer a cytological means of mapping. rather than trying to accurately count individual chiasmata, we have examined properties of the mathematical relationship between frequencies of nonadorned disomic configurations in meiosi ...	1996	18469936
the alcohol dehydrogenase genes of cotton.	we report here the initial characterization of the alcohol dehydrogenase (adh) gene family of the allotetraploid gossypium hirsutum, a crop plant that is highly sensitive to waterlogging. twelve adh cdnas were isolated from a library constructed from rna prepared from anaerobically stressed root tips. nine of the twelve cdnas fell into one class, while each of the other three cdnas fell into a separate class. the 3'-untranslated regions had little or no homology between classes implying that eac ...	1996	8806419
distribution of 5s and 18s-28s rdna loci in a tetraploid cotton (gossypium hirsutum l.) and its putative diploid ancestors.	the most widely cultivated species of cotton, gossypium hirsutum, is a disomic tetraploid (2n=4x=52). it has been proposed previously that extant a- and d-genome species are most closely related to the diploid progenitors of the tetraploid. we used fluorescent in situ hybridization (fish) to determine the distribution of 5s and 18s-28s rdna loci in the a-genome species g. herbaceum and g. arboreum, the d-genome species g. raimondii and g. thurberi, and the ad tetraploid g. hirsutum. high signal- ...	1996	8662259
specific binding of a verticillium dahliae phytotoxin to protoplasts of cotton, gossypium hirsutum.	the occurrence of specific, high-affinity binding sites for a protein-lipopolysaccharide (plp) phytotoxin purified from culture filtrates of a virulent vertidllium dahliae isolate has been demonstrated in cotton protoplasts. binding of the (125)i-radiolabelled plp-complex to protoplasts from cotyledon tissue was saturable and with an affinity (kd = 17.3 nm) comparable with the concentration required for biological activity. a single class of binding site, accessible at the surface of the intact ...	1996	24178171
establishment of molecular markers and linkage groups in two f2 populations of upland cotton.	two f2 populations of cotton (gossypium hirsutum l.) from the crosses of hs46 x marcabucag8us-1-88 (mar) and hs46 x pee dee 5363 (pd5363) were characterized for restriction fragment length polymorphisms (rflps) using dna probes. seventy-three probe/enzyme combinations were used in the hs46 x mar population analysis, which resulted in 42 informative polymorphic fragments. these 42 moleclar markers represented 26 polymorphic loci, which consisted of 15 codominant and 11 dominant (+/-) genotypes. c ...	1996	24166617
volatile semiochemicals released from undamaged cotton leaves (a systemic response of living plants to caterpillar damage).	cotton plants (gossypium hirsutum l.), attacked by herbivorous insects release volatile semiochemicals (chemical signals) that attract natural enemies of the herbivores to the damaged plants. we found chemical evidence that volatiles are released not only at the damaged site but from the entire cotton plant. the release of volatiles was detected from upper, undamaged leaves after 2 to 3 d of continuous larval damage on lower leaves of the same plant. compounds released systemically were (z)-3-he ...	1996	12226304
characterization and expression of metallothionein-like genes in cotton.	we have characterized cotton (gossypium hirsutum l.) genes encoding type 1 metallothionein-like proteins that are highly expressed in roots. little or no expression of these genes was detected in other organs and tissues. the deduced amino acid sequences have a high degree of similarity with type 1 metallothionein-like proteins from other plants, including a central hydrophobic domain flanked by conserved cysteine-rich motifs. the type 1 metallothionein-like genes of cotton are encoded by a smal ...	1996	8790303
systemic induction of feeding deterrents in cotton plants by feeding ofspodoptera spp. larvae.	cotton,gossypium hirsutum l., has been shown to exhibit systemic induced resistance to arthropods under certain conditions. we conducted experiments to determine the effects of previous feeding ofspodoptera exigua hübner andspodoptera littoralis (boisd.) larvae on feeding behavior, growth, and survival of larvae subsequently feeding on cotton. in one feeding choice test,s. exigua larvae preferred young leaves from undamaged control plants to undamaged young leaves from a previously damaged plant ...	1996	24227615
changing the dimensions of suberin lamellae of green cotton fibers with a specific inhibitor of the endoplasmic reticulum-associated fatty acid elongases.	the fibers of the green lint mutant of cotton (gossypium hirsutum l.) contain large amounts of wax and are suberized. more than 96% of the bifunctional aliphatic suberin monomers ([alpha],[omega]-alkanedioic acids and [omega]-hydroxyalkanoic acids) have chain lengths of c22 and c24 in green cotton fiber suberin. in fibers grown in the presence of s-ethyl-n,n-dipropylthiocarbamate (eptc), a specific inhibitor of the endoplasmic reticulum-associated fatty acid elongases, the aliphatic suberin mono ...	1996	12226192
structural characterization of genes corresponding to cotton fiber mrna, e6: reduced e6 protein in transgenic plants by antisense gene.	two genes, each corresponding to fiber mrna e6, were isolated from cotton cultivars coker 312 (gossypium hirsutum l.) and sea island (g. barbadense l.). e6 is one of the predominant fiber-specific mrnas present during early fiber development. the distinguishing feature of nucleotide-derived e6 protein is the presence of a motif where a dimer, ser-gly, is repeated several times. two of the sea island genes contained a pentameric motif, ser-gly, while one of the coker genes had one and the other h ...	1996	8616253
sucrose synthase localization during initiation of seed development and trichome differentiation in cotton ovules.	sucrose synthase in cotton (gossypium hirsutum l.) ovules was immunolocalized to clarify the relationship between this enzyme and (a) sucrose import/utilization during initiation of seed development, (b) trichome differentiation, and (c) cell-wall biosynthesis in these rapidly elongating "fibers." analyses focused on the period immediately before and after trichome initiation (at pollination). internal tissues most heavily immunolabeled were the developing nucellus, adjacent integument (inner su ...	1995	12228669
diversity in cyclic sesquiterpene production by gossypium hirsutum.	major sesquiterpene components of oil of texas race stock 810 of gossypium hirsutum were alpha- and beta-selinene. this is the seventh cyclic terpene type found to date in this genus. both alpha- and beta-selinene, along with aromadendrene, were found but only as minor components of extracts of several domestic cultivars of g. hirsutum.	1995	8590634
genetic fingerprinting of australian cotton cultivars with rapd markers.	rapd (random amplified polymorphic dna) markers generated by 30 random decamer primers were used to fingerprint 12 released cultivars and a breeding line of gossypium hirsutum and 1 cultivar of g. barbadense presently under cultivation in australia. among a total of 453 developed markers, 69 (15.2%) were only present (unique) in the g. barbadense cultivar pima s-7. of the remaining markers, 128 (33.3%) were fixed in all 13 g. hirsutum cultivars. in pairwise comparisons of the degree of band shar ...	1995	18470223
a membrane-associated form of sucrose synthase and its potential role in synthesis of cellulose and callose in plants.	sucrose synthase (susy; ec 2.4.1.13; sucrose + udp reversible udpglucose + fructose) has always been studied as a cytoplasmic enzyme in plant cells where it serves to degrade sucrose and provide carbon for respiration and synthesis of cell wall polysaccharides and starch. we report here that at least half of the total susy of developing cotton fibers (gossypium hirsutum) is tightly associated with the plasma membrane. therefore, this form of susy might serve to channel carbon directly from sucro ...	1995	7568131
n-acylphosphatidylethanolamine in dry and imbibing cottonseeds. amounts, molecular species, and enzymatic synthesis.	n-acylphosphatidylethanolamine (nape), an unusual acylated derivative of phosphatidylethanolamine (pe), was recently shown to be synthesized from pe and free fatty acids in cotyledons of cotton (gossypium hirsutum l.) seedlings (k.d. chapman, t.s. moore [1993] plant physiol 102: 761-769). here we report that nape is present in dry seeds of cotton and increases with time of imbibition from 2.31 nmol/seed in dry seeds to 4.26 nmol/seed in 4-h-soaked seeds. total phospholipid/seed also increased su ...	1995	7480326
solubilization and partial characterization of extensin fragments from cell walls of cotton suspension cultures. evidence for a covalent cross-link between extensin and pectin.	extensin, a major hydroxyproline (hyp)-rich glycoprotein in walls of cultured cells of dicotyledonous plants, is very difficult to solubilize. to learn about the nature of the insolubilization, we have tested the ability of a variety of selective hydrolytic methods, and combinations of them, to liberate extensin or fragments of extensin from suspension-culture cell walls. after the complete deglycosylation of cotton (gossypium hirsutum l.) walls, trypsinization solubilized 80% of the hyp. the se ...	1995	7659756
expression of two related vacuolar h(+)-atpase 16-kilodalton proteolipid genes is differentially regulated in a tissue-specific manner.	the 16-kd proteolipid subunit is the principal integral membrane protein of the vacuolar h(+)-atpase (v-atpase) complex that forms the proton channel responsible for translocating protons across lipid bilayers. two degenerate synthetic oligonucleotides, cot11 and cot12, corresponding to highly conserved transmembrane domains in all 16-kd subunits sequenced so far, were used to amplify a partial cdna of the v-atpase proteolipid subunit from cotton (gossypium hirsutum l.) by polymerase chain react ...	1995	7659746
characterization of mrna for a proline-rich protein of cotton fiber.	cotton (gossypium hirsutum l.) mrna (h6) is expressed predominantly in fiber cells and is present during early primary cell wall formation. however, h6 protein is found to accumulate during later stages, when active secondary cell wall formation occurs, indicating possible regulation at the translational level and function in the secondary cell wall assembly. the nucleotide-derived amino acid sequence of pck-h6 is proline rich (35 mol %) with a calculated molecular mass of 21 kd. cotton protein ...	1995	7610164
a cdna encoding ribosomal protein s4e from cotton (gossypium hirsutum l.).		1995	7784518
characterization of a cotton (gossypium hirsutum l.) fiber mrna (fb-b6).		1995	7770543
[beta]-glucan synthesis in the cotton fiber (iv. in vitro assembly of the cellulose i allomorph).	in vitro assembly of cellulose from plasma membrane extracts of the cotton (gossypium hirsutum) fiber was enriched by a combination of 3-(n-morpholino)propanesulfonic acid extraction buffer and two independent digitonin solubilization steps consisting of 0.05% digitonin (se1) followed by 1% digitonin (se2). glucan synthase activity assays revealed that, although the se2 fraction possessed higher activity, only 8.6% of the in vitro product survived acetic/nitric acid treatment. on the other hand, ...	1995	12228346
the distribution of gossypium hirsutum chromatin in g. barbadense germ plasm: molecular analysis of introgressive plant breeding.	cotton is unusual among major crop plants in that two cross-fertile species are widely cultivated for a common economic product, fiber. both historical evidence and classical genetic studies suggest that many improved forms of gossypium barbadense ("sea island", "egyptian", and "pima" cottons) may include chromatin derived from g. hirsutum. using 106 restriction fragment length polymorphism (rflp) loci well distributed across the cotton genome, we revealed the amount and genomic distribution of ...	1995	24170011
organization, inheritance and expression of acetohydroxyacid synthase genes in the cotton allotetraploid gossypium hirsutum.	the acetohydroxyacid synthase (ahas) gene family of the cotton ad allotetraploid gossypium hirsutum has been cloned and characterized. we have identified six different ahas genes from an analysis of genomic clones and southern blots of genomic dna. four of the six genes are organized as tandem pairs, in which the genes are separated by only 2-3 kb. conservation of restriction fragment length polymorphisms between g. hirsutum and a-genome and d-genome-containing diploid cottons was sufficient to ...	1995	7640356
differential induction of 3-hydroxy-3-methylglutaryl coa reductase in two cotton species following inoculation with verticillium.	gossypium barbadense cottons are typically more resistant to wilt pathogens than are gossypium hirsutum cultivars. both species make terpenoid phytoalexins in response to infection, implicating isoprenoid biosynthesis as a factor in resistance. conserved regions in plant 3-hydroxy-3-methylglutaryl coenzyme a reductase (hmgr), the first enzyme in the terpene biosynthesis pathway, were used to design polymerase chain reaction primers for cloning a fragment of a cotton hmgr gene. the clone was used ...	1995	8664497
protease inhibitors of manduca sexta expressed in transgenic cotton.	to explore the effectiveness of insect derived protease inhibitors in protecting plants against insect feeding, anti-trypsin, anti-chymotrypsin and anti-elastase protease inhibitor (pi) genes from manduca sexta l. were expressed in transgenic cotton (gossypium hirsutum l.). from 198 independent transformants, 35 elite lines were further analyzed. under the control of the 35s promoter of camv, pi accumulated to approximately 0.1% of total protein, depending on the tissue analyzed. using cell-flow ...	1995	24186707
influence of meloidogyne incognita on the water relations of cotton grown in microplots.	the effects of meloidogyne incognita on the growth and water relations of cotton were evaluated in a 2-year field study. microplots containing methyl bromide-fumigated fine sandy loam soil were infested with the nematode and planted to cotton (gossypium hirsutum l.). treatments included addition of nematodes alone, addition of nematodes plus the insecticide-nematicide aldicarb (1.7 kg/ha), and an untreated control. meloidogyne incognita population densities reached high levels in both treatments ...	1995	19277313
effect of tropical rotation crops on meloidogyne incognita and other plant-parasitic nematodes.	in a field experiment conducted on sandy soil in florida during the 1993 season, rotation crops of castor (ricinus communis), velvetbean (mucuna deeringina), 'mississippi silver' cowpea (vigna unguiculata), american jointvetch (aeschynomene americana), 'dehapine 51' cotton (gossypium hirsutum), and 'sx-17' sorghum-sudangrass (sorghum bicolor x s. sudanense) were effective in maintaining low population densities (<12/100 cm(3) soil) of meloidogyne incognita race 1, whereas high population densiti ...	1995	19277319