Publications

blood volume required for detection of low levels and ultralow levels of organisms responsible for neonatal bacteremia by use of bactec peds plus/f, plus aerobic/f medium, and the bd bactec fx system: an in vitro study.	we used an in vitro technique to investigate blood volumes required to detect bacteremia and fungemia with low concentrations of an organism. at 1 to 10 cfu/ml, escherichia coli, staphylococcus epidermidis, staphylococcus aureus, listeria monocytogenes, candida albicans, and candida parapsilosis isolates were detected in volumes as low as 0.5 ml. detection of streptococcus agalactiae and detection of bacteremia at <1 cfu/ml were unreliable.	2015	26292299
broad spectrum anti-microbial compounds producing bacteria from coast of qingdao bays.	anti-microbial resistance burden and hazard associated with chemical treatment of infections demanded for new anti-microbial natural products. marine associated microorganisms are the enormous source of bioactive compounds. in this study we have isolated 272 marine bacteria among them 136 (50%) were antagonistic to at least one of the four pathogenic strains listeria monocytogenes, vibrio cholerae, e. coli and s. aureus. only two strains exhibited antibacterial activity against all four test str ...	2015	25730803
effectiveness of inactivation of foodborne pathogens during simulated home pan frying of steak, hamburger or meat strips.	in order to evaluate the effect of simulated home pan frying of raw meat and meat preparations of different animal species on the thermal inactivation of pathogens, the heat resistance (d-value) of three strains of campylobacter jejuni, escherichia coli o157:h7, salmonella spp., listeria monocytogenes and two strains of generic e. coli was validated in bhi and adjusted bhi (i.e. ph5.6 and 1.5% nacl) at 60°c. the d-values were obtained of the linear phase of the survivor curves created in ginafit ...	2015	26004267
effect of oxygen stress on growth and survival of clostridium perfringens, campylobacter jejuni, and listeria monocytogenes under different storage conditions.	this study investigated the growth and survival of three foodborne pathogens (clostridium perfringens, campylobacter jejuni, and listeria monocytogenes) in beef (7% fat) and nutrient broth under different oxygen levels. samples were tested under anoxic (<0.5%), microoxic (6 to 8%), and oxic (20%) conditions during storage at 7 °c for 14 days and at 22 °c for 5 days. two initial inoculum concentrations were used (1 and 2 log cfu per g of beef or per ml of broth). the results show that c. perfring ...	2015	25836393
antibiotic susceptibility and prevalence of foodborne pathogens in poultry meat in romania.	the occurrence of pathogenic strains in poultry meat is of growing concern in romania. another problem found on a global level is the continuous increase of antimicrobial resistance in bacteria isolated from food. this study aimed to evaluate the prevalence of pathogenic bacteria in poultry carcasses obtained in romania in 2012-2013 and to reveal the most prevalent patterns of antimicrobial resistance in the isolated strains.	2015	25596569
[regularities of the ubiquitous polyhostal microorganisms selection by the example of three taxa].	the investigation of the bacterial populations' heterogeneity contributes to the control of natural foci, causative agents of nosocomial infections, to the analysis of the microbial evolution. multilocus sequence typing (mlst) was employed for the analysis of the diversity and features of the distribution of polyhostal ubiquitous microorganisms of the genera burkholderia, leptospira, and listeria, which belong to three bacterial phyla: proteobacteria, spirochaetes, and firmicutes. according to t ...	2015	26107896
experimental application of lactobacillus fermentum ccm 7421 in combination with chlorophyllin in dogs.	chlorophyll belongs in a larger class of phytochemical plant pigments currently receiving more attention as a physiologically active dietary component. although most research has focused on its biological activities such as its antioxidant, antimutagenic, anti-inflammatory or apoptotic effects in humans or rodents, there is limited knowledge at this time about the combinative possibilities of chlorophyll with probiotic bacteria. our aim was to test the growth characteristics of canine-derived pr ...	2015	26062536
isolation and taxonomic identity of bacteriocin-producing lactic acid bacteria from retail foods and animal sources.	bacteriocin-producing (bac⁺) lactic acid bacteria (lab) were isolated from a variety of food products and animal sources. samples were enriched in de man, rogosa, and sharpe (mrs) lactocilli broth and plated onto mrs agar plates using a "sandwich overlay" technique. inhibitory activity was detected by the "deferred antagonism" indicator overlay method using listeria monocytogenes as the primary indicator organism. antimicrobial activity against l. monocytogenes was detected by 41 isolates obtain ...	2015	27682080
comparison of desiccation tolerance among listeria monocytogenes, escherichia coli o157:h7, salmonella enterica, and cronobacter sakazakii in powdered infant formula.	bacterial pathogens such as listeria monocytogenes, escherichia coli o157:h7, salmonella enterica, and cronobacter sakazakii have demonstrated long-term survival in/on dry or low-water activity (aw) foods. however, there have been few comparative studies on the desiccation tolerance among these bacterial pathogens separately in a same food matrix. in the present study, the survival kinetics of the four bacterial pathogens separately inoculated onto powdered infant formula as a model low-aw food ...	2015	25581184
the sactibiotic subclass of bacteriocins: an update.	the sactibiotics are a recently designated subclass of bacteriocins that contain characteristic cysteine sulphur to α -carbon linkages mediated through post-translational modifications. they are a relatively small subclass of bacteriocins compared to the most thoroughly studied lantibiotics. the sactibiotics that have been extensively studied thus far are thuricin cd, subtilosin a, thurincin h, and propionicin f. despite their recent discovery, there have already been significant advances made i ...	2015	26031307
rna-based fluorescent biosensors for live cell imaging of second messenger cyclic di-amp.	cyclic di-amp (cdia) is a second messenger predicted to be widespread in gram-positive bacteria, some gram-negative bacteria, and archaea. in the human pathogen listeria monocytogenes, cdia is an essential molecule that regulates metabolic function and cell wall homeostasis, and decreased levels of cdia result in increased antibiotic susceptibility. we have generated fluorescent biosensors for cdia through fusion of the spinach2 aptamer to ligand-binding domains of cdia riboswitches. the biosens ...	2015	25965978
in vitro probiotic characteristics of lactobacillus plantarum zdy 2013 and its modulatory effect on gut microbiota of mice.	lactobacillus plantarum zdy 2013, a novel strain isolated from chinese traditional fermented acid beans, was systematically evaluated for its survival capacity under stress conditions (ph, bile salt, simulated gastrointestinal tract, and antibiotics), production of exopolysaccharide and antagonism against 8 pathogens. its effect on mice gut microbiota was also investigated by quantitative pcr and pcr-denaturing gradient gel electrophoresis. the results showed that zdy 2013 can grow at ph 3.5 and ...	2015	26142853
an assessment of the human health impact of seven leading foodborne pathogens in the united states using disability adjusted life years.	we explored the overall impact of foodborne disease caused by seven leading foodborne pathogens in the united states using the disability adjusted life year (daly). we defined health states for each pathogen (acute illness and sequelae) and estimated the average annual incidence of each health state using data from public health surveillance and previously published estimates from studies in the united states, canada and europe. these pathogens caused about 112 000 dalys annually due to foodborn ...	2015	25633631
development and application of loop-mediated isothermal amplification assays based on its-1 for rapid detection of toxoplasma gondii in pork.	the loop-mediated isothermal amplification (lamp) assay is a novel method that rapidly amplifies dna with high specificity and sensitivity under isothermal conditions. in this study, we established a lamp assay with six primers targeting a highly conserved region of toxoplasma gondii its-1 sequence. the amplification protocol completes within 30 min under isothermal condition in a 65°c water bath while specificity tests confirmed no cross-reactivity with dna templates of neospora caninum, eimeri ...	2015	25624074
phenotypic complementation of genetic immunodeficiency by chronic herpesvirus infection.	variation in the presentation of hereditary immunodeficiencies may be explained by genetic or environmental factors. patients with mutations in hoil1 (rbck1) present with amylopectinosis-associated myopathy with or without hyper-inflammation and immunodeficiency. we report that barrier-raised hoil-1-deficient mice exhibit amylopectin-like deposits in the myocardium but show minimal signs of hyper-inflammation. however, they show immunodeficiency upon acute infection with listeria monocytogenes, ...	2015	25599590
tuberculosis and the risk of infection with other intracellular bacteria: a population-based study.	summary persons who develop tuberculosis (tb) may have subtle immune defects that could predispose to other intracellular bacterial infections (icbis). we obtained data on tb and five icbis (chlamydia trachomatis, salmonella spp., shigella spp., yersinia spp., listeria monocytogenes) reported to the tennessee department of health, usa, 2000-2011. incidence rate ratios (irrs) comparing icbis in persons who developed tb and icbis in the tennessee population, adjusted for age, sex, race and ethnici ...	2015	25148655
alanine-scanning mutational analysis of durancin gl reveals residues important for its antimicrobial activity.	durancin gl is a novel class iia bacteriocin with 43 residues produced by enterococcus durans 41d. this bacteriocin demonstrates narrow inhibition spectrum and potent antimicrobial activity against several listeria monocytogenes strains, including nisin-resistant l. monocytogenes nr30. a systematic alanine-scanning mutational analysis with site-directed mutagenesis was performed to analyze durancin gl residues important for antimicrobial activity and specificity. results showed that three mutati ...	2015	26168032
genetic diversity and some aspects of antimicrobial activity of lactic acid bacteria isolated from goat milk.	lactic acid bacteria (lab, n = 57) were previously obtained from raw goat milk, identified as lactococcus spp. (n = 24) and enterococcus spp. (n = 33), and characterized as bacteriocinogenic. fingerprinting by pulsed field gel electrophoresis (pfge) demonstrated high genetic diversity, and 30 strains were selected and exhibited strong antimicrobial activity against 46 target strains (lab, spoilage, and foodborne pathogens). six strains (lactococcus lactis: glc03 and glc05; and enterococcus duran ...	2015	25637509
transfer, composition and technological characterization of the lactic acid bacterial populations of the wooden vats used to produce traditional stretched cheeses.	the biofilms of 12 wooden vats used for the production of the traditional stretched cheeses caciocavallo palermitano and pdo vastedda della valle del belìce were investigated. salmonella spp. and listeria monocytogenes were never detected. total coliforms were at low numbers with escherichia coli found only in three vats. coagulase-positive staphylococci (cps) were below the enumeration limit, whereas lactic acid bacteria (lab) dominated the surfaces of all vats. in general, the dominance was sh ...	2015	26338114
characterization of lactobacillus fermentum pl9988 isolated from healthy elderly korean in a longevity village.	in this work, we wanted to develop a probiotic from famous longevity villages in korea. we visited eight longevity villages in korea to collect fecal samples from healthy adults who were aged above 80 years and had regular bowel movements, and isolated lactic-acid-producing bacteria from the samples. isolated colonies that appeared on mrs agar containing bromophenol blue were identified by means of 16s rrna sequencing, and 102 of the isolates were identified as lactic-acid-producing bacteria (18 ...	2015	26095384
probiotic features of lactobacillus strains isolated from ragusano and pecorino siciliano cheeses.	in the present study 177 lactobacillus spp. strains, isolated from ragusano and pecorino siciliano cheeses, were in vitro screened for probiotic traits, and their characteristics were compared to those of lactobacillus rhamnosus gg, commercial strain. based on acidic and bile salt resistance, thirteen lactobacillus strains were selected. the multiplex-pcr application revealed that nine strains belonged to l. rhamnosus species and four to lactobacillus paracasei species. all selected strains were ...	2015	25998823
antimicrobial action of oleanolic acid on listeria monocytogenes, enterococcus faecium, and enterococcus faecalis.	this study investigated the antimicrobial action of oleanolic acid against listeria monocytogenes, enterococcus faecium, and enterococcus faecalis. to determine the cytotoxicity of oleanolic acid, hep-2 cells were incubated with oleanolic acid at 37°c. mics (minimal inhibition concentrations) for l. monocytogenes, e. faecium, and e. faecalis were determined using two-fold microdilutions of oleanolic acid, and bacterial cell viability was then assessed by exposing the bacteria to oleanolic acid a ...	2015	25756202
behavior of listeria monocytogenes in a multi-species biofilm with enterococcus faecalis and enterococcus faecium and control through sanitation procedures.	the formation of mono-species biofilm (listeria monocytogenes) and multi-species biofilms (enterococcus faecium, enterococcus faecalis, and l. monocytogenes) was evaluated. in addition, the effectiveness of sanitation procedures for the control of the multi-species biofilm also was evaluated. the biofilms were grown on stainless steel coupons at various incubation temperatures (7, 25 and 39°c) and contact times (0, 1, 2, 4, 6 and 8 days). in all tests, at 7°c, the microbial counts were below 0.4 ...	2015	25655573
extraction, characterization and antimicrobial activity of sulfated polysaccharides from fish skins.	sulfated polysaccharides were extracted from gray triggerfish (gtsp) and smooth hound (shsp) skins. their chemical and physical characteristics were determined using x-ray diffraction and infrared spectroscopic analysis. the antibacterial activities of gtsp and shsp against listeria monocytogenes (atcc 43251), staphylococcus aureus (atcc 25923), enterococcus faecalis (atcc 29212), escherichia coli (atcc 25922), salmonella enterica (atcc 43972) and enterobacter sp were evaluated by determining cl ...	2015	25647621
detection of listeria monocytogenes with short peptide fragments from class iia bacteriocins as recognition elements.	we employed a direct peptide-bacteria binding assay to screen peptide fragments for high and specific binding to listeria monocytogenes. peptides were screened from a peptide array library synthesized on cellulose membrane. twenty four peptide fragments (each a 14-mer) were derived from three potent anti-listerial peptides, leucocin a, pediocin pa1, and curvacin a, that belong to class iia bacteriocins. fragment leu10 (geafsagvhrlang), derived from the c-terminal region of leucocin a, displayed ...	2015	25548942
bacteriocin protein bacl1 of enterococcus faecalis targets cell division loci and specifically recognizes l-ala2-cross-bridged peptidoglycan.	bacteriocin 41 (bac41) is produced from clinical isolates of enterococcus faecalis and consists of two extracellular proteins, bacl1 and baca. we previously reported that bacl1 protein (595 amino acids, 64.5 kda) is a bacteriolytic peptidoglycan d-isoglutamyl-l-lysine endopeptidase that induces cell lysis of e. faecalis when an accessory factor, baca, is copresent. however, the target of bacl1 remains unknown. in this study, we investigated the targeting specificity of bacl1. fluorescence micros ...	2015	25368300
antimicrobial and antioxidant activities of enterococcus species isolated from meat and dairy products.	lactic acid bacteria (lab) have an important role in a great variety of fermented foods. in addition to their contribution to sensory characteristics, they enhance food preservation and can be used as probiotics. in this study, the antimicrobial and antioxidant activities of culture supernatants and cell free extracts of 16 lab isolated from meat and dairy products were investigated. the bacterial were identified by 16s rrna sequencing. genbank blast analysis revealed that all the isolates belon ...	2015	26675908
[biological activity of antimicrobial peptides of enterococcus faecium].	isolate bacteriocins from enterococcus faecium metabolites and characterize their effect on cells of gram positive (listeria monocytogenes) and gram negative (escherichia coli) bacteria.	2015	26470413
antimicrobial activity and the presence of virulence factors and bacteriocin structural genes in enterococcus faecium cm33 isolated from ewe colostrum.	screening of lactic acid bacteria (lab) isolated from ewe colostrum led to the identification and isolation of enterococcus faecium cm33 with interesting features like high survival rates under acidic or bile salts condition, high tolerance for the simulated gastrointestinal condition, and high adhesive potential to caco-2 cells. according the inhibition of pathogen adhesion test results, this strain can reduce more than 50% adhesion capacity of escherichia coli, shigella flexneri, klebsiella pn ...	2015	26284059
experimental studies of microbial populations and incidence of zoonotic pathogens in the faeces of red deer (cervus elaphus).	wild animals can serve as hosts, amplifiers or reservoirs for various zoonotic diseases. most species of deer in highly fragmented agricultural landscapes, search out maximum cover from intrusive human activity. hence, the likelihood of zoonosis transmission is likely to increase the more humans and wildlife interact. in our study, we conducted a comparative analysis of bacteria isolated from the faeces of red deer (cervus elaphus) living in their natural environment in south-western poland and ...	2015	26222832
a microplate growth inhibition assay for screening bacteriocins against listeria monocytogenes to differentiate their mode-of-action.	lactic acid bacteria (lab) have historically been used in food fermentations to preserve foods and are generally-recognized-as-safe (gras) by the fda for use as food ingredients. in addition to lactic acid; some strains also produce bacteriocins that have been proposed for use as food preservatives. in this study we examined the inhibition of listeria monocytogenes 39-2 by neutralized and non-neutralized bacteriocin preparations (bac+ preps) produced by lactobacillus curvatus fs47; lb. curvatus ...	2015	26111195
in vitro protective effect of lactic acid bacteria on listeria monocytogenes adhesion and invasion of caco-2 cells.	the adhesion of listeria monocytogenes to intestinal endothelial cells is a crucial step in the infection process, which is not well understood. in this study, we evaluated the potential ability of bacteriocin-producing enterococcus faecium, leuconostoc mesenteroides and lactobacillus sakei strains to prevent the adhesion and invasion of eukaryotic cells by ten different l. monocytogenes isolates. the results showed that e. faecium 130 co-cultured with l. monocytogenes was the most effective in ...	2015	25672897
antilisterial effect of bacteriocin sh01, obtained from enterococcus faecium sh01, in ground beef.	from the previous study, enterococcus faecium sh01 was isolated from mukeunji, an over-ripened kimchi, and it produced bacteriocin sh01. bacteriocin sh01 showed an inhibitory effect against listeria monocytogenes atcc 19111, a bacterial strain causing human listeriosis. crude bacteriocin sh01 was purified by ammonium sulfate precipitation and its inhibitory activity at two concentrations (500 and 1,000 au/g) against listeria monocytogenes atcc 19111 was investigated in ground beef at increasing ...	2015	26761830
potentially probiotic and bioprotective lactic acid bacteria starter cultures antagonise the listeria monocytogenes adhesion to ht29 colonocyte-like cells.	the capability of five lactic acid bacteria (lab) to counteract the adhesion of listeria monocytogenes to the epithelial intestinal cell line ht29 was studied. the highest adhesion ability to ht29 was achieved by the intestinal strain lactobacillus rhamnosus ctc1679, followed by the meat-derived strains lactobacillus sakei ctc494 and enterococcus faecium ctc8005. surprisingly, the meat strains showed significantly better adhesion to ht29 than two faecal isolates of lactobacillus casei and even s ...	2015	25488261
effects of listeria monocytogenes egd-e and salmonella enterica ser. typhimurium lt2 chitinases on intracellular survival in dictyostelium discoideum and mammalian cell lines.	some bacterial pathogens produce chitinases as virulence factors during host infection. the molecular target of such enzymes in non-chitinous hosts remains uncertain. we studied the importance of listeria monocytogenes egd-e and salmonella enterica ser. typhimurium lt2 chitinases for intracellular survival in dictyostelium discoideum, and for salmonella, also infection of mammalian cell lines, and a mouse model. the salmonella chitinase did not contribute significantly to infection of d. discoid ...	2015	25908871
improved properties and microbiological safety of novel cottage cheese containing spices.	the study focuses on developing novel cottage cheese containing spices with acceptable sensory properties, increased biological value and extended shelf life. thirty types of cheese with added fresh or dried parsley, dill, pepper, garlic and rosemary were produced. characterisation of phenolic compounds, antioxidant capacity and antibacterial activity of spices and cheese samples were evaluated. the cheese containing fresh pepper and fresh and dried herbs showed excellent sensory properties, wit ...	2015	27904380
combined effect of irradiation and ageing condition on physicochemical and microbial quality of hanwoo eye of round.	the combined effects of electron-beam irradiation and ageing of beef were examined. the irradiated samples at dose of 0 or 2 kgy were kept and analyzed for the microbial growth, shear values, meat color, and nucleotide-related flavor compounds at different ageing temperatures (2, 10, or 25℃) for 8 d. the irradiation effect on inactivation of foodborne pathogens was also investigated. the population of listeria monocytogenes and e. coli o157:h7 inoculated in beef samples decreased in proportion t ...	2015	26761855
technological properties and probiotic potential of lactobacillus fermentum strains isolated from west african fermented millet dough.	throughout africa, food fermentations are still driven by indigenous microorganisms which influence the nutritional, organoleptic and safety of the final products. however, for improved safety, consistent quality and beneficial health effects, a trend has emerged which involves the isolation of indigenous strains from traditional fermented products to be used as functional starter cultures. these functional starter cultures possess inherent functional characteristics and can contribute to food q ...	2015	26560346
vital signs: multistate foodborne outbreaks - united states, 2010-2014.	millions of u.s. residents become ill from foodborne pathogens each year. most foodborne outbreaks occur among small groups of persons in a localized area. however, because many foods are distributed widely and rapidly, and because detection methods have improved, outbreaks that occur in multiple states and that even span the entire country are being recognized with increasing frequency.	2015	26540483
uv-heat treatments for the control of foodborne microbial pathogens in chicken broth.	this investigation established the process criteria for using uv-c light and mild heat (uv-h treatment) to inactivate 5-log10 cycles (performance criterion) of common foodborne pathogen populations, escherichia coli, salmonella typhimurium, listeria monocytogenes, and staphylococcus aureus, when inoculated in chicken broth. to define the target microorganism and the proper uv-h treatment conditions (including uv dose, treatment time, and temperature) that would achieve the stated performance cri ...	2015	26539493
impact of t300a variant of atg16l1 on antibacterial response, risk of culture positive infections, and clinical course of crohn's disease.	autophagy-related 16-like 1 (atg16l1) deficiency leads to impaired cellular autophagy and bacterial degradation as well as an altered cytokine production. the single-nucleotide polymorphism rs2241880 (t300a) is associated with an increased risk for crohn's disease (cd). atg16l1 polymorphisms could therefore have an impact on the risk of infectious complications and disease course in cd. we examined the impact of the t300a genotype on the antibacterial response toward a panel of pathogenic bacter ...	2015	26673830
microbiological and shelf life characteristics of eviscerated and vacuum packed freshwater catfish (ompok pabda) during chill storage.	the effect of vacuum packaging technique on the shelflife of eviscerated pabda catfish (ompok pabda) during chill storage at 4 ± 2 °c was studied. the shelflife of pabda fish was predicted based on the cumulative observations of biochemical, microbiological and sensory parameters. there was no significant difference in the lipid quality parameters studied between the samples during the chill storage period, whereas, vacuum packaging significantly improved the sensory and microbiological quality. ...	2015	25745210
ceramide and sphingosine in pulmonary infections.	acid sphingomyelinase and ceramide have previously been shown to play a central role in infections with neisseria gonorrhoeae, staphylococcus aureus, listeria monocytogenes, pseudomonas aeruginosa, salmonella typhimurium, escherichia coli, and mycobacterium avium. recent studies have extended the role of sphingolipids in bacterial infections and have demonstrated that ceramide and sphingosine are central to the defense of lungs against bacterial pathogens. ceramide accumulates in the airway epit ...	2015	25720061
prevalence of bacteria and absence of anisakid parasites in raw and prepared fish and seafood dishes in spanish restaurants.	this study evaluated the presence of bacteria and anisakid parasites in 45 samples of raw anchovies in vinegar, a dish widely eaten in spain, and in 227 samples of cooked fish and cephalopods served in spanish food service establishments. our analysis showed that, according to european and spanish regulation, 14 to 30% of the prepared fish and cephalopod dishes exceeded the maximum allowable level for mesophilic aerobic counts, and 10 to 40% of these samples exceeded the allowable levels for ent ...	2015	25719890
first two domains at the lp_1643 protein n terminus inhibit pathogen adhesion to porcine mucus in vitro.	gastrointestinal probiotics are important members of intestinal microflora in both healthy animals and human beings, and these bacteria may reduce the risk of infection caused by certain opportunistic pathogens through exclusive inhibition, competition, and displacement. the lp_1643 protein on the cell surface of lactobacillus plantarum wcfsi was assumed to possess a mucus-binding capability. this study aimed to determine if purified his-n2 protein exclusively inhibits pathogen adhesion to porci ...	2015	25710153
(aminophosphane)gold(i) and silver(i) complexes as antibacterial agents.	this manuscript describes the synthesis of new au(i) and ag(i) complexes with aminophosphane ligands and a study of their antibacterial activity against gram-negative salmonella enterica serovar typhimurium and escherichia coli and gram-positive listeria monocytogenes and staphylococcus aureus. the bactericidal assays revealed the effectiveness of these compounds on paradigm gram-negative and gram-positive pathogens, showing a moderate antimicrobial activity, comparable with the antibiotics of r ...	2015	25706322
the expression of propionicin plg-1 gene (plg-1) by lactic starters.	propionicin plg-1 is a bacteriocin produced by propionibacterium thoenii p127. such bacteriocin inhibits wide range of food-borne pathogens such as pathogenic escherichia coli, pseudomonas aeruginosa, vibrio parahaemolyticus, yersinia enterocolitica and a strain of corynebacterium sp. in the present study, plg-1 gene expressing propionicin plg-1 was isolated, sequenced for the first time and the resulting sequence was analysed using several web-based bioinformatics programs. the pcr product cont ...	2015	25684078
a 3-year hygiene and safety monitoring of a meat processing plant which uses raw materials of global origin.	a systematic approach in monitoring the hygiene of a meat processing plant using classical microbiological analyses combined with molecular characterization tools may assist in the safety of the final products. this study aimed: (i) to evaluate the total hygiene level and, (ii) to monitor and characterize the occurrence and spread of salmonella spp. and listeria monocytogenes in the environment and the final products of a meat industry that processes meat of global origin. in total, 2541 samples ...	2015	25600954
analytical bioconjugates, aptamers, enable specific quantitative detection of listeria monocytogenes.	as a major human pathogen in the listeria genus, listeria monocytogenes causes the bacterial disease listeriosis, which is a serious infection caused by eating food contaminated with the bacteria. we have developed an aptamer-based sandwich assay (absa) platform that demonstrates a promising potential for use in pathogen detection using aptamers as analytical bioconjugates. the whole-bacteria selex (wb-selex) strategy was adopted to generate aptamers with high affinity and specificity against li ...	2015	25590973
storage stability and antibacterial activity of eugenol nanoliposomes prepared by an ethanol injection-dynamic high-pressure microfluidization method.	eugenol is a major phenolic component with diverse biological activities. however, it is difficult to formulate into an aqueous solution due to poor water solubility, and this limits its application. in the present study, eugenol nanoliposomes (en) were prepared by combining the ethanol injection method with the dynamic high-pressure microfluidization method. good physicochemical characterizations of en were obtained. the successful encapsulation of eugenol in nanoliposomes was confirmed by four ...	2015	25581174
the effect of surface properties of polycrystalline, single phase metal coatings on bacterial retention.	in the food industry microbial contamination of surfaces can result in product spoilage which may lead to potential health problems of the consumer. surface properties can have a substantial effect on microbial retention. the surface characteristics of chemically different coatings (cu, ti, mo, ag, fe) were defined using white light profilometry (micro-topography and surface features), atomic force microscopy (nano-topography) and physicochemical measurements. the ag coating had the greatest top ...	2015	25576986
the microbiological quality of ready-to-eat salads in turkey: a focus on salmonella spp. and listeria monocytogenes.	the microbiological safety of ready-to-eat (rte) foods is of special concern as they are not exposed to further processing before consumption. in the present study, listeria monocytogenes and salmonella spp. were isolated from 15(6%) and 21(8%) samples respectively out of 261 rte foods commercialized in turkey. escherichia coli was present in 10(4%) samples analyzed. psychrotrophic aerobic populations >6logcfu/g were found in 36 (14%) of the samples, while total coliforms were detected in 155 (5 ...	2015	25528536
antimicrobial activity of hop extracts against foodborne pathogens for meat applications.	the objective of this study was the fundamental investigation of the antimicrobial efficiency of various hop extracts against selected foodborne pathogens in vitro, as well as their activity against listeria monocytogenes in a model meat marinade and on marinated pork tenderloins.	2015	25494620
inactivation kinetics of foodborne pathogens by uv-c radiation and its subsequent growth in fresh-cut kailan-hybrid broccoli.	the inactivation of escherichia coli, s. enteritidis and listeria monocytogenes after uv-c radiation with 0, 2.5, 5, 7.5, 10 and 15 kj uv-c m(-2) on fresh-cut kailan-hybrid broccoli was explored. inactivation did not follow linear kinetics. hence, it was modelled by using the weibull distribution function, obtaining adjusted r(2) values higher than 94%, indicative of the accuracy of the model to the experimental data. the uv-c doses needed to reduce 1 log cycle the e. coli, s. enteritidis and l. ...	2015	25475295
evaluation of pathogen inactivation on sliced cheese induced by encapsulated atmospheric pressure dielectric barrier discharge plasma.	pathogen inactivation induced by atmospheric pressure dielectric barrier discharge (dbd) (250 w, 15 khz, air discharge) produced in a rectangular plastic container and the effect of post-treatment storage time on inactivation were evaluated using agar plates and cheese slices. when agar plates were treated with plasma, populations of escherichia coli, salmonella typhimurium, and listeria monocytogenes showed 3.57, 6.69, and 6.53 decimal reductions at 60 s, 45 s, and 7 min, respectively. when the ...	2015	25475265
the influence of the cell free solution of lactic acid bacteria on tyramine production by food borne-pathogens in tyrosine decarboxylase broth.	the function of cell-free solutions (cfss) of lactic acid bacteria (lab) on tyramine and other biogenic amine production by different food borne-pathogens (fbps) was investigated in tyrosine decarboxylase broth (tdb) using hplc. cell free solutions were prepared from four lab strains. two different concentrations which were 50% (5 ml cfs+5 ml medium/1:1) and 25% (2.5 ml cfs+7.5 ml medium/1:3) cfs and the control without cfs were prepared. both concentration of cfs of streptococcus thermophilus a ...	2015	25465993
prevalence and predictors of bacterial meningitis in young infants with fever without a source.	classical criteria differ when performing cerebrospinal fluid (csf) analysis in infants younger than 90 days with fever without a source (fws). our objectives were to analyze the prevalence and microbiology of bacterial meningitis in this group and its prevalence in relation to clinical and laboratory risk factors.	2015	25461476
antimicrobial effects of short chained imidazolium-based ionic liquids—influence of anion chaotropicity.	ionic liquids (ils), a new solvent class composed solely of ions, have already found their way into numerous chemical and biochemical applications. due to their unique properties and wide application range, research utilizing this new technology for biotechnological applications is steadily increasing. however, progress is hampered by lack of toxicological data, especially concerning il anions and their general underlying toxicity mechanisms. the present study investigated for the first time the ...	2015	25450920
brazilian fruit pulps as functional foods and additives: evaluation of bioactive compounds.	eight tropical fruit pulps from brazil were simultaneously characterised in terms of their antioxidant and antimicrobial properties. antioxidant activity was screened by dpph radical scavenging activity (126-3987 mg te/100g dw) and ferric reduction activity power (368-20819 mg aae/100g dw), and complemented with total phenolic content (329-12466 mg gae/100g dw) and total flavonoid content measurements (46-672 mg ee /100g dw), whereas antimicrobial activity was tested against the most frequently ...	2015	25442579
attachment of escherichia coli to listeria monocytogenes for pediocin-mediated killing.	listeria phage endolysin cell wall-binding domain (cbd) from the listeria phage a500 was fused with flagellar subunit flic in escherichia coli, aiming at binding of e. coli cells to listeria cells, followed by enhanced killing of listeria by pediocin production. flic::cbd chimeric flagella were expressed and detected by western blot. however, only few chimeric flagella could be isolated from the recombinant cells compared with sufficient amount of wild-type flagella obtained from the host cells. ...	2015	25270683
prevalence of major foodborne pathogens in food confiscated from air passenger luggage.	the eu has issued several directives and regulations pertaining to the importation of animals and products of animal origin (poao) and veterinary controls on importation. unfortunately, little information is available concerning associated risks and no attempts have been made to collect baseline data on the actual prevalence of zoonotic agents in poao carried by travellers. to meet these challenges the eu recently introduced and financed a research project "promise". its main objectives were to ...	2015	25161012
ixodes ricinus defensins attack distantly-related pathogens.	antimicrobial peptides are ubiquitous components of eukaryotic innate immunity. defensins are a well-known family of antimicrobial peptides, widely distributed in ticks, insects, plants and mammals, showing activity against bacteria, viruses, fungi, yeast and protozoan parasites. ixodes ricinus is the most common tick species in europe and is a vector of pathogens affecting human and animal health. recently, six defensins (including two isoforms) were identified in i. ricinus. we investigated th ...	2015	26255244
a microfluidic droplet digital pcr for simultaneous detection of pathogenic escherichia coli o157 and listeria monocytogenes.	sensitive and rapid identification of pathogenic bacterial is extremely important due to the serious threat of pathogens to human health. in this study, we demonstrate the simultaneous and sensitive detection of pathogenic escherichia coli o157 and listeria monocytogenes using a novel duplex droplet digital pcr (ddpcr) platform. the ddpcr platform, which uses a mineral oil-saturated polydimethylsiloxane (osp) chip to overcome the problem of droplet evaporation, integrates the functions of drople ...	2015	26226346
determining the minimum ripening time of artisanal minas cheese, a traditional brazilian cheese.	physical, physicochemical, and microbiological changes were monitored in 256 samples of artisanal minas cheese from eight producers from serro region (minas gerais, brazil) for 64 days of ripening to determine the minimum ripening time for the cheese to reach the safe microbiological limits established by brazilian legislation. the cheeses were produced between dry season (april-september) and rainy season (october-march); 128 cheeses were ripened at room temperature (25 ± 4 °c), and 128 were ri ...	2015	26221111
simultaneous electrochemical magneto genosensing of foodborne bacteria based on triple-tagging multiplex amplification.	simultaneous detection of salmonella enterica, listeria monocytogenes and escherichia coli based on triple-tagging multiplex pcr and electrochemical magneto genosensing on silica magnetic particles is reported. a set of tagging primers were selected for the specific amplification of yfir (375 bp), hlya (234 bp) and eaea (151bp), being one of the primers for each set labelled with fluorescein, biotin and digoxigenin coding for s. enterica, l. monocytogenes and e. coli, respectively. afterwards, e ...	2015	26201982
microbiological safety and food handling practices of seed sprout products in the australian state of victoria.	seed sprouts have been implicated as vehicles for numerous foodborne outbreaks worldwide. seed sprouts pose a unique food safety concern because of the ease of microbiological seed contamination, the inherent ability of the sprouting process to support microbial growth, and their consumption either raw or lightly cooked. to examine seed sprout safety in the australian state of victoria, a survey was conducted to detect specific microbes in seed sprout samples and to investigate food handling pra ...	2015	26197293
molecular insights into a molluscan transferrin homolog identified from disk abalone (haliotis discus discus) evidencing its detectable role in host antibacterial defense.	the basic function of transferrin is to bind iron (iii) ions in the medium and to deliver them to the locations where they are required for metabolic processes. it also takes part in the host immune defense mainly via its ability to bind to iron (iii) ions. hence, transferrin is also identified as an important acute-phase protein in host immunity. abalones are major shellfish aquaculture crops that are susceptible to a range of marine microbial infections. since transferrin is known to be a majo ...	2015	26191782
preparation and application of agar/alginate/collagen ternary blend functional food packaging films.	ternary blend agar/alginate/collagen (a/a/c) hydrogel films with silver nanoparticles (agnps) and grapefruit seed extract (gse) were prepared. their performance properties, transparency, tensile strength (ts), water vapor permeability (wvp), water contact angle (ca), water swelling ratio (sr), water solubility (ws), and antimicrobial activity were determined. the a/a/c film was highly transparent, and both agnps and gse incorporated blend films (a/a/c(agnps) and a/a/c(gse)) exhibited uv-screenin ...	2015	26187189
laminarin from irish brown seaweeds ascophyllum nodosum and laminaria hyperborea: ultrasound assisted extraction, characterization and bioactivity.	ultrasound assisted extraction (uae), purification, characterization and antioxidant activity of laminarin from irish brown seaweeds ascophyllum nodosum and laminarina hyperborea were investigated. uae was carried out using 60% ultrasonic power amplitude and 0.1 m hydrochloric acid for 15 min. separately, solid-liquid extraction was carried in an orbital shaker using 0.1 m hydrochloric acid at 70 °c for 2.5 h. uae with hydrochloric acid resulted in the highest concentration of laminarin, 5.82% a ...	2015	26184235
influence of general stress-response alternative sigma factors σ(s) (rpos) and σ(b) (sigb) on bacterial tolerance to the essential oils from origanum vulgare l. and rosmarinus officinalis l. and pulsed electric fields.	this study assessed the influence of general stress-response alternative sigma factors rpos (σ(s)) and sigb (σ(b)) on tolerance of escherichia coli (e. coli mg1655 and its isogenic mutant e. coli mg1655 δrpos) and listeria monocytogenes (l. monocytogenes egd-e and its isogenic mutant l. monocytogenes egd-e δsigb) to the essential oils (eos) from origanum vulgare l.-oregano (oveo) and rosmarinus officinalis l.-rosemary (roeo), as well as the changes in tolerance of parental and δrpos and δsigb mu ...	2015	26159473
inactivation of pathogenic bacteria inoculated onto a bacto™ agar model surface using tio2-uvc photocatalysis, uvc and chlorine treatments.	the aim of this study was to study inactivation of different pathogenic bacteria on agar model surface using tio2-uv photocatalysis (tuvp).	2015	26099167
cold plasma inactivation of internalised bacteria and biofilms for salmonella enterica serovar typhimurium, listeria monocytogenes and escherichia coli.	microbial biofilms and bacteria internalised in produce tissue may reduce the effectiveness of decontamination methods. in this study, the inactivation efficacy of in-package atmospheric cold plasma (acp) afterglow was investigated against salmonella typhimurium, listeria monocytogenes and escherichia coli in the forms of planktonic cultures, biofilms formed on lettuce and associated bacteria internalised in lettuce tissue. prepared lettuce broth (3%) was inoculated with bacteria resulting in a ...	2015	26093991
amino acid mediated synthesis of silver nanoparticles and preparation of antimicrobial agar/silver nanoparticles composite films.	silver nanoparticles (agnps) were synthesized using amino acids (tyrosine and tryptophan) as reducing and capping agents, and they were incorporated into the agar to prepare antimicrobial composite films. the agnps solutions exhibited characteristic absorption peak at 420 nm that showed a red shift to ∼434 nm after forming composite with agar. xrd data demonstrated the crystalline structure of agnps with dominant (111) facet. apparent surface color and transmittance of agar films were greatly in ...	2015	26076636
bacterial enteric infections among older adults in the united states: foodborne diseases active surveillance network, 1996-2012.	a growing segment of the population-adults aged ≥65 years-is more susceptible than younger adults to certain enteric (including foodborne) infections and experience more severe disease.	2015	26067228
impact of cell-free supernatant of lactic acid bacteria on putrescine and other polyamine formation by foodborne pathogens in ornithine decarboxylase broth.	conversion of ornithine to putrescine by salmonella paratyphi a, listeria monocytogenes, staphylococcus aureus, and escherichia coli was investigated in ornithine decarboxylase broth (odb) using cell-free supernatants (cfss) obtained from leuconostoc mesenterodies subsp. cremoris, pediococcus acidilactici, lactococcus lactis subsp. lactis, streptococcus thermophilus. two groups of cell-free supernatants (25 or 50%) and control (only odb) were prepared to investigate putrescine (put) and other po ...	2015	26043863
irrigation is significantly associated with an increased prevalence of listeria monocytogenes in produce production environments in new york state.	environmental (i.e., meteorological and landscape) factors and management practices can affect the prevalence of foodborne pathogens in produce production environments. this study was conducted to determine the prevalence of listeria monocytogenes, listeria species (including l. monocytogenes), salmonella, and shiga toxin-producing escherichia coli (stec) in produce production environments and to identify environmental factors and management practices associated with their isolation. ten produce ...	2015	26038903
epidemiology of bacteremia in previously healthy febrile infants: a follow-up study.	describe the etiology of bacteremia among a geographically diverse sample of previously well infants with fever admitted for general pediatric care and to characterize demographic and clinical characteristics of infants with bacteremia according to bacterial etiology. we hypothesized that the epidemiology of bacteremia in febrile infants from a geographically diverse cohort would show similar results to smaller or single-center cohorts previously reported.	2015	26034160
libyan thymus capitatus essential oil: antioxidant, antimicrobial, cytotoxic and colon pathogen adhesion-inhibition properties.	in the present work, the libyan wild-growing thymus capitatus essential oil (eo) was evaluated for its biological properties.	2015	26033505
detection of poultry meat specific bacteria using ftir spectroscopy and chemometrics.	ftir spectra of poultry meat specific bacteria viz. salmonella enteritidis, pseudomonas ludensis, listeria monocytogenes and escherichia coli were collected and investigated for identification of spectral windows capable of bacterial classification and quantification. two separate datasets obtained at different times were used in the study to check reproducibility of results. multivariate data analysis techniques viz. principal component analysis (pca), partial least-squares discriminant analysi ...	2015	26028771
a comparison of in-house real-time lamp assays with a commercial assay for the detection of pathogenic bacteria.	molecular detection of bacterial pathogens based on lamp methods is a faster and simpler approach than conventional culture methods. although different lamp-based methods for pathogenic bacterial detection are available, a systematic comparison of these different lamp assays has not been performed. in this paper, we compared 12 in-house real-time lamp assays with a commercialized kit (isothermal master mix) for the detection of listeria monocytogenes, salmonella spp, staphylococcus aureus, esche ...	2015	26020698
in vitro generation of rabbit anti-listeria monocytogenes monoclonal antibody using single cell based rt-pcr linked cell-free expression systems.	rabbit monoclonal antibodies (mabs) have advantages over mouse antibodies in diagnostics and biotechnological applications owing to higher affinity and specificity.we developed a platform to generate rabbit mabs by a novel monoclonal antibody generation method named “single-cell reverse transcription-pcr linked in vitro-expression (sicrex)” system. in this method,we use single-cell based rt-pcr followed by sequential pcr steps of mab genes and subsequent cell-free protein synthesis (cfps) by usi ...	2015	26454028
survival and high-hydrostatic pressure inactivation of foodborne pathogens in salmorejo, a traditional ready-to-eat food.	salmorejo is a traditional tomato-based creamy product. because salmorejo is not heat-processed, there is a risk of contamination with foodborne pathogens from raw materials. even though bacterial growth in salmorejo is strongly inhibited because of its acidic ph (close to 3.9), the growth and survival of 3 foodborne pathogens in this food has not been studied before. in this study, 3 cocktails consisting of escherichia coli o157, salmonella enterica serovar enteritidis, and listeria monocytogen ...	2015	26448479
astaxanthin from psychrotrophic sphingomonas faeni exhibits antagonism against food-spoilage bacteria at low temperatures.	food production and processing industry holds a perpetual relationship with microorganisms and their by-products. in the present study, we aimed to identify beneficial cold-adapted bacteria devoid of any food spoilage properties and study their antagonism against common food-borne pathogens at low temperature conditions. ten isolates were obtained on selective isolation at 5 °c, which were spread across genera pseudomonas, sphingomonas, psychrobacter, leuconostoc, rhodococcus, and arthrobacter. ...	2015	26411893
persistence of indicator and pathogenic microorganisms in broccoli following manure spreading and irrigation with fecally contaminated water: field experiment.	in 2011 and 2012, trials consisting of experimental plots were carried out to evaluate the presence of pathogenic (listeria monocytogenes, salmonella) and prevalence of indicator (escherichia coli) microorganisms in broccoli fertilized with liquid hog manure or mineral fertilizers and irrigated zero, one, or two times with e. coli-contaminated water. in 2011, results showed that e. coli contamination in broccoli heads was affected by the interval between irrigation and sampling (p = 0.0236), wit ...	2015	26408125
ready-to-eat street-vended food as a potential vehicle of bacterial pathogens and antimicrobial resistance: an exploratory study in porto region, portugal.	the ready-to-eat street vending commerce, as street mobile food vendors, has grown exponentially worldwide, representing in some countries a significant proportion of food consumed by the urban population. however, the microbiological food safety hazards of mobile vending units in industrialized countries are scarcely evaluated. to assess the microbiological quality and safety of this type of food and try to achieve the connection of its contamination with hygienic conditions of food-handlers, w ...	2015	25910073
rapid detection of listeria monocytogenes in milk using confocal micro-raman spectroscopy and chemometric analysis.	listeria monocytogenes is a facultatively anaerobic, gram-positive, rod-shape foodborne bacterium causing invasive infection, listeriosis, in susceptible populations. rapid and high-throughput detection of this pathogen in dairy products is critical as milk and other dairy products have been implicated as food vehicles in several outbreaks. here we evaluated confocal micro-raman spectroscopy (785 nm laser) coupled with chemometric analysis to distinguish six closely related listeria species, inc ...	2015	25863337
assessment of microbial risk factors and impact of meteorological conditions during production of baby spinach in the southeast of spain.	there is a timely need to evaluate the effect agricultural factors and meteorological conditions on fresh produce contamination. this study evaluated those risk factors and described, for the first time, the distribution of indicator microorganisms (escherichia coli, enterococcus, coliforms, and enterobacteriaceae) and the prevalence of foodborne pathogens (enterohaemorrhagic e. coli, listeria monocytogenes and salmonella spp.) in baby spinach grown in the southeast of spain. a longitudinal stud ...	2015	25846928
effects of sub-lethal concentrations of thyme and oregano essential oils, carvacrol, thymol, citral and trans-2-hexenal on membrane fatty acid composition and volatile molecule profile of listeria monocytogenes, escherichia coli and salmonella enteritidis.	the aim of this work was to investigate the modifications of cell membrane fatty acid composition and volatile molecule profiles of listeria monocytogenes, salmonella enteritidis, escherichia coli, during growth in the presence of different sub-lethal concentrations of thyme and oregano essential oils as well as carvacrol, thymol, trans-2-hexenal and citral. the results evidenced that the tested molecules induced noticeable modifications of membrane fatty acid profiles and volatile compounds pro ...	2015	25842326
determining the potential link between irrigation water quality and the microbiological quality of onions by phenotypic and genotypic characterization of escherichia coli isolates.	the potential transfer of human pathogenic bacteria present in irrigation water onto fresh produce was investigated, because surface water sources used for irrigation purposes in south africa have increasingly been reported to be contaminated with enteric bacterial pathogens. a microbiological analysis was performed of a selected river in limpopo province, south africa, that is often contaminated with raw sewage from municipal sewage works and overhead irrigated onions produced on a commercial f ...	2015	25836387
comparison of the prevalences and diversities of listeria species and listeria monocytogenes in an urban and a rural agricultural watershed.	foods and related processing environments are commonly contaminated with the pathogenic listeria monocytogenes. to investigate potential environmental reservoirs of listeria spp. and l. monocytogenes, surface water and point source pollution samples from an urban and a rural municipal water supply watershed in nova scotia, canada, were examined over 18 months. presumptive listeria spp. were cultured from 72 and 35% of rural and urban water samples, respectively, with 24% of the positive samples ...	2015	25819965
listeria monocytogenes-carrying consortia in food industry. composition, subtyping and numerical characterisation of mono-species biofilm dynamics on stainless steel.	in order to find out how real listeria monocytogenes-carrying biofilms are in industrial settings, a total of 270 environmental samples belonging to work surfaces from fish (n = 123), meat (n = 75) and dairy industries (n = 72) were analysed in order to detect l. monocytogenes. 12 samples were positive for l. monocytogenes and a total of 18 different species were identified as accompanying microbiota in fish and meat industry. no l. monocytogenes was found in samples from dairy industry. molecul ...	2015	26001376
sublethal injury and virulence changes in listeria monocytogenes and listeria innocua treated with antimicrobials carvacrol and citral.	the aim of this study was to evaluate the effect of two antimicrobial substances, carvacrol and citral, on listeria monocytogenes and listeria innocua cells, as well as possible virulence changes in injured cells, using caenorhabditis elegans as a model test. the results indicated that the percentage of sublethal damage was higher in l. monocytogenes than in l. innocua. the results of the study carried out by using c. elegans indicated that c. elegans fed in a lawn of l. monocytogenes previously ...	2015	25998809
microbiological shelf life at different temperatures and fate of listeria monocytogenes and escherichia coli inoculated in unflavored and strawberry yogurts.	three different trials were performed on unflavored and strawberry yogurts produced in a small-scale dairy plant. in the first trial, the microbiological shelf life of the products was evaluated at 4, 8, and 20°c. at 4°c the product showed low total viable counts until the end of the trial (d 35=3.0±0.7 and 1.5±0.0 log cfu/g in unflavored and strawberry yogurt, respectively). the loads were lower in strawberry yogurt at 4°c compared with unflavored yogurt because of the antimicrobial activity ex ...	2015	25981065
microbiological quality of milk sold directly from producers to consumers.	the aim of this study was to determine the microbiological quality of raw cow milk from direct sale points. raw cow milk samples were collected from 5 randomly selected direct sale points for microbiological evaluation. the samples were analyzed to determine total aerobic bacterial count, somatic cell count (scc), counts of enterobacteriaceae, enterococcus, escherichia coli, and staphylococcus, and presence of salmonella, listeria monocytogenes, and inhibitory substances. the mean counts of tota ...	2015	25958278
the antibiotic activity and mechanisms of sugarcane (saccharum officinarum l.) bagasse extract against food-borne pathogens.	sugarcane bagasse contains natural compositions that can significantly inhibit food-borne pathogens growth. in the present study, the phenolic content in sugarcane bagasse was detected as higher than 4 mg/g dry bagasse, with 470 mg quercetin/g polyphenol. the sugarcane bagasse extract showed bacteriostatic activity against the growth of staphylococcus aureus, listeria monocytogenes, escherichia coli and salomonella typhimurium. additionally, the sugarcane bagasse extract can increase the electri ...	2015	25952848
control of pathogens in biofilms on the surface of stainless steel by levulinic acid plus sodium dodecyl sulfate.	the efficacy of levulinic acid (lva) plus sodium dodecyl sulfate (sds) to remove or inactivate listeria monocytogenes, salmonella typhimurium, and shiga toxin-producing escherichia coli (stec) in biofilms on the surface of stainless steel coupons was evaluated. five- or six-strain mixtures (ca. 9.0 log cfu/ml) of the three pathogens were separately inoculated on stainless steel coupons. after incubation at 21 °c for 72 h, the coupons were treated for 10 min by different concentrations of lva plu ...	2015	25950851
improvement of antimicrobial activity of pediocin pa-1 by site-directed mutagenesis in c-terminal domain.	pediocin pa-1 is a well-known class iia bacteriocin which shows strong inhibitory effect against listeria monocytogenes. in this work, in order to improve the antimicrobial activity, eight single- site mutants and six combination mutants on nine interesting sites in the c-terminal region of pediocin pa-1 were constructed and expressed in escherichia coli heterologously. ten mutants demonstrated enhancement activity when performed the agar diffusion test to the indicator strain l. monocytogenes. ...	2015	26299998
control of escherichia coli and listeria monocytogenes in suckling-lamb meat evaluated using microbial challenge tests.	escherichia coli and listeria monocytogenes microbial challenge tests were performed on fresh suckling-lamb meat. hind leg slices were chilly stored under two modified atmosphere packaging (map) environments (a: 15%o2/60%co2/25%n2, b: 15%o2/30%co2/55%n2) and vacuum packaging (v). only e. coli was reduced between 0.72-1.25 log cfu/g from day 1 to day 4 by the combined use of map/v, chilling storage and the growth of native lactic acid bacteria. however, l. monocytogenes was not inhibited by the a ...	2015	26298670
treatment strategies for central nervous system infections: an update.	central nervous system infection continues to be an important cause of mortality and morbidity worldwide. our incomplete knowledge on the pathogenesis of how meningitis-causing pathogens cause cns infection and emergence of antimicrobial resistance has contributed to the mortality and morbidity. an early empiric antibiotic treatment is critical for the management of patients with bacterial meningitis, but early recognition of bacterial meningitis continues to be a challenge.	2015	25328149
prevalence and characterization of listeria monocytogenes, salmonella and shiga toxin-producing escherichia coli isolated from small mexican retail markets of queso fresco.	queso fresco (qf) is a handmade cheese consumed and produced in latin america. in mexico, qf production is associated with a microbiological risk. the aim of the study was to determine the incidence and characterization of listeria monocytogenes, salmonella spp., and shiga toxin-producing escherichia coli (stec) in qf from retail markets of the north-western state of sinaloa, mexico, and to assess the effect of physicochemical parameters on listeria presence. a total of 75 qf samples were obtain ...	2015	24809389