Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| evolution of clostridia and streptomycetes in full-scale composting facilities and pilot drums equipped with on-line temperature monitoring and aeration. | the evolution of sporulating bacteria in full-scale composting facilities with online temperature monitoring has been poorly studied, although organic matter recycling increases. we analysed clostridium perfringens and sulphite-reducing clostridia (src) by cultivation, and streptomycetes by real-time pcr in five full-scale, temperature-monitored and aerated composting processes, and two pilot-scale drum composters. facilities composted woodchips, sawdust, peat, or bark amended sludge or source-s ... | 2011 | 21719280 |
| structure of the food-poisoning clostridium perfringens enterotoxin reveals similarity to the aerolysin-like pore-forming toxins. | clostridium perfringens enterotoxin (cpe) is a major cause of food poisoning and antibiotic-associated diarrhea. upon its release from c. perfringens spores, cpe binds to its receptor, claudin, at the tight junctions between the epithelial cells of the gut wall and subsequently forms pores in the cell membranes. a number of different complexes between cpe and claudin have been observed, and the process of pore formation has not been fully elucidated. we have determined the three-dimensional stru ... | 2011 | 21839091 |
| eradication of chemotherapy-resistant cd44+ human ovarian cancer stem cells in mice by intraperitoneal administration of clostridium perfringens enterotoxin. | background: emerging evidence has suggested that the capability to sustain tumor formation, growth, and chemotherapy resistance in ovarian as well as other human malignancies exclusively resides in a small proportion of tumor cells termed cancer stem cells. during the characterization of cd44(+) ovarian cancer stem cells, we found a high expression of the genes encoding for claudin-4. because this tight junction protein is the natural high-affinity receptor for clostridium perfringens enterotoxi ... | 2011 | 21692061 |
| factors associated with the quality of well water and the prevalence of waterborne diseases in the municipality of abomey-calavi in benin. | the objective of this work is to assess the factors associated with the water quality of household wells, suggest solutions to improve it, and study the prevalence of water-borne diseases in this community. the quality of well water and the prevalence of waterborne diseases in the municipality were studied by analysis of the microbiological indicators currently used to assess drinking-water quality and the retrospective study of waterborne diseases treated in the local health centres. the wells ... | 2011 | 21700557 |
| effects of necrotic enteritis challenge on intestinal micro-architecture and mucin profile. | 1. this study investigated the effect of eimeria spp./clostridium perfringens induced necrotic enteritis and traditional antibiotic preventatives on intestinal micro-architecture and mucin profile. 2. a total of 600 cobb 500 broiler chickens were randomly assigned to the following three groups: (i) unchallenged, (ii) challenged, and (iii) zinc bacitracin/monensin (znb/monensin) (n = 25 chickens/pen, 8 pens/group). the challenged and znb/monensin chickens were individually inoculated with eimeria ... | 2011 | 21919578 |
| microbial safety of air-dried and rewetted biosolids. | to assess microbial safety of treated sewage sludge (biosolids), we examined the inactivation of microbial indicators for potential bacterial, viral and protozoan pathogens. the levels of indicators were determined throughout the air-drying and storage phases of anaerobically digested sewage sludge. samples were collected from two wastewater treatment plants (wwtps) in victoria, australia. established methods were applied for analysis of bacteria and coliphages, based on membrane filtration and ... | 2011 | 21942204 |
| effect of direct-fed microbials on performance and clostridium perfringens colonization of turkey poults. | clostridium perfringens is recognized as an enteric pathogen in humans, domestic animals, and livestock. this organism is associated with necrotic enteritis, gangrenous dermatitis, clostridial dermatitis (turkeys), and gizzard erosions in poultry. this study was conducted to evaluate the effectiveness of a direct-fed microbial (dfm), primalac (star labs, clarksdale, mo), in preventing intestinal colonization of turkey poults with c. perfringens. one-day-old turkey poults (n = 128) were randomly ... | 2011 | 22010255 |
| the effect of probiotics on faecal microbiota and genotoxic activity of faecal water in patients with atopic dermatitis: a randomized, placebo-controlled study. | background: colonic microbiota is involved in the etiology of colon cancer according to several reports. studies also indicate that the microbiota differs between atopic patients and healthy subjects. objective: to evaluate whether a probiotic mix containing lactobacillus paracasei lpc-37, lactobacillus acidophilus 74-2, and bifidobacterium animalis subsp. lactis dgcc 420 can affect the microbiota and its genotoxic activity in healthy subjects and patients with atopic dermatitis (ad). methods: a ... | 2011 | 21963389 |
| acid phosphatase test proves superior to standard phenotypic identification procedure for clostridium perfringens strains isolated from water. | clostridium perfringens is used as an indicator for persistent faecal pollution as well as to monitor the efficacy of water treatment processes. for these purposes, differentiation between c. perfringens and other clostridia is essential and is routinely carried out by phenotypic standard tests as proposed in the iso/cd 6461-2:2002 (iso_lgmn: lactose fermentation, gelatine liquidation, motility and nitrate reduction). because the iso_lgmn procedure is time consuming and labour intensive, the aci ... | 2011 | 21872622 |
| [Molecular characterization and antimicrobial resistance of Clostridium perfringens isolates of different origins from Costa Rica]. | Clostridium perfringens, a Gram positive, spore-forming anaerobe, is widely distributed in nature. Based upon their production of four major toxins alpha, beta, epsilon and iota, C. perfringens is classified into five toxinotypes (A-E). Some strains produce an enterotoxin (CPE), encoded by the cpe gene, which causes diarrhea in humans and some animals. C. perfringens strains that had been previously isolated and been kept at -80 degrees C were analyzed for the presence of toxin genes and for ant ... | 2011 | 22208067 |
| Structural analysis of CPF_2247, a novel a-amylase from Clostridium perfringens. | CPF_2247 from Clostridium perfringens ATCC 13124 was identified as a putative carbohydrate-active enzyme by its low sequence identity to endo-ß-1,4-glucanases belonging to family 8 of the glycoside hydrolase classification. The X-ray crystal structure of CPF_2247 determined to 2.0 Å resolution by single-wavelength anomalous dispersion using seleno-methionine-substituted protein revealed an (a/a)(6) barrel fold. A large cleft on the surface of the protein contains residues that are structurally c ... | 2011 | 21905105 |
| characterization and distribution of the gene cluster encoding rumc, an anti-clostridium perfringens bacteriocin produced in the gut. | ruminococcin c (rumc) is a trypsin-dependent bacteriocin produced by ruminococcus gnavus e1, a gram-positive strict anaerobic strain isolated from human feces. it consists of at least three similar peptides active against clostridium perfringens. in this article, a 15-kb region from r. gnavus e1 chromosome, containing the biosynthetic gene cluster of rumc was characterized. it harbored 17 open reading frames (called rum(c) genes) with predicted functions in bacteriocin biosynthesis and post-tran ... | 2011 | 22092178 |
| mechanism of clostridium perfringens enterotoxin interaction with claudin-3/-4 protein suggests structural modifications of the toxin to target specific claudins. | claudins (cld) are essential constituents of tight junctions. domain i of clostridium perfringens enterotoxin (ccpe) binds to the second extracellular loop (ecl2) of a subset of claudins, e.g. cld3/4 and influences tight junction formation. we aimed to identify interacting interfaces and to alter claudin specificity of ccpe. mutagenesis, binding assays, and molecular modeling were performed. mutation-guided ecl2 docking of cld3/4 onto the crystal structure of ccpe revealed a common orientation o ... | 2011 | 22128179 |
| Selection of herbal therapeutics against deltatoxin mediated Clostridial infections. | Clostridium perfringens (a versatile pathogenic bacterium) secretes enterotoxins (the deltatoxin, virulent factor) and causes food borne gastroenteritis and gasgangrene. The organism was isolated and characterized from improperly cooked meat and poultry samples. The isolated organism showed multiple drug resistance indicating that the treatment is challenging. Hence, there is need for improved therapeutic agents. The rational design of improved therapeutics requires the crystal structure for the ... | 2011 | 21904424 |
| Functional insight into the C-terminal extension of halolysin SptA from haloarchaeon Natrinema sp. J7. | Halolysin SptA from haloarchaeon Natrinema sp. J7 consists of a subtilisin-like catalytic domain and a C-terminal extension (CTE) containing two cysteine residues. In this report, we have investigated the function of the CTE using recombinant enzymes expressed in Haloferax volcanii WFD11. Deletion of the CTE greatly reduced but did not abolish protease activity, which suggests that the CTE is not essential for enzyme folding. Mutational analysis suggests that residues Cys303 and Cys338 within th ... | 2011 | 21886797 |
| effects of non-fermented and fermented soybean milk intake on faecal microbiota and faecal metabolites in humans. | the effects of non-fermented soybean milk (nfsm) and fermented soybean milk (fsm) intake on the faecal microbiota and metabolic activities in 10 healthy volunteers were investigated. soybean oligosaccharides, raffinose and stachyose were utilized by bifidobacteria except for bifidobacterium bifidum, but most strains of escherichia coli and clostridium perfringens could not use them. during the dietary administration of fsm, the number of bifidobacteria and lactobacilli in the faeces increased (p ... | 2011 | 22040525 |
| aberrant structures of fecal bacterial community in allergic infants profiled by 16s rrna gene pyrosequencing. | we investigated the correlation between fecal bacteria composition in early infancy and the prevalence of allergic diseases in late infancy. the fecal microbiota in the first 2 months was profiled using the 16s rrna v6 short-tag sequences in the community and statistically compared between two groups of subjects who did and did not show allergic symptoms in the first 2 years (n = 11 vs. 11). in the allergic group, genus bacteroides at 1 month and genera propionibacterium and klebsiella at 2 mont ... | 2011 | 22029688 |
| validation of a numerical indicator of microbial contamination for karst springs. | rapid changes in spring water quality in karst areas due to rapid recharge of bacterially contaminated water are a major concern for drinking water suppliers and users. the main objective of this study was to use field experiments with fecal indicators to verify the vulnerability of a karst spring to pathogens, as determined by using a numerical modeling approach. the groundwater modeling was based on linear storage models that can be used to simulate karst water flow. the vulnerability of the k ... | 2011 | 20180864 |
| clostridium perfringens epsilon toxin is cytotoxic for human renal tubular epithelial cells. | clostridium perfringens epsilon toxin (etx) is responsible for a fatal enterotoxemia in different animal species, producing extensive renal damage, neurological disturbance and edema of lungs, heart and kidneys. however, there is no information about the susceptibility of humans to etx. here, we report that primary cultures of human renal tubular epithelial cells (hrtec) exposed to etx showed a marked swelling with subsequent large blebs surrounding most cells. the incubation of hrtec with etx p ... | 2011 | 20488848 |
| effects of age and region on fecal microflora in elderly subjects living in bama, guangxi, china. | intestinal microflora analysis was performed on 52 healthy elderly subjects of different ages and in different regions in bama county, guangxi, china. the participants were assigned to three groups depending on their age and location: longevous (group m; mean age = 98 years; n = 21); rural younger elderly (group s; mean age = 70 years; n = 18); and urban elderly (group c; mean age = 82 years; n = 13). ten groups of bacteria were quantified using real-time pcr. age-related differences were observ ... | 2011 | 20509028 |
| on-farm multi-contamination of pigs by food-borne bacterial zoonotic hazards: an exploratory study. | application of risk analysis to control pork-borne zoonoses on farms is a major aim of the current european food safety legislation. the prevalence, levels of contamination and variations in pig contaminations according to herds and batches must therefore be studied to define relevant methods for control of zoonoses. the aims of this exploratory study were to provide information on the prevalence and levels of infection of finisher/fattening pigs by campylobacter spp., clostridium perfringens, l ... | 2011 | 20655671 |
| a 17-year review of foodborne outbreaks: describing the continuing decline in england and wales (1992-2008). | systematic national surveillance of foodborne disease outbreaks effectively serves the development of public health policy on food safety. the health protection agency has maintained a collaborative surveillance system for foodborne outbreaks in england and wales since 1992. up to 2008, 2429 foodborne outbreaks were identified, described and analysed for changes over time. salmonella spp. accounted for half of the outbreaks, although the proportion of these decreased over the surveillance period ... | 2011 | 20696086 |
| oral administration of clostridium butyricum for modulating gastrointestinal microflora in mice. | this study aimed to evaluate the safety of clostridium butyricum and to investigate the effect of c. butyricum on mice ecosystem in the intestinal tract by way of examining the population of different microorganisms isolated from caecal contents. we firstly evaluated the safety of c. butyricum using acute toxicity test and ames test. then forty male balb/c mice were divided into the following four treatment groups, each consisting of ten mice: normal group, low-dose group, medium-dose group and ... | 2011 | 20711781 |
| pathogen removal in farm-scale psychrophilic anaerobic digesters processing swine manure. | this study assessed the efficiency of commercial-scale psychrophilic anaerobic digestion in sequencing batch reactors (padsbrs) for pathogen removal from pig manure. the impact of treatment cycle length and of hydraulic flow regimes on pathogen removal efficiency was investigated. two conventionally operated sbrs (br1 and br2) and two sbrs simultaneously fed during the draw step (br3 and br4) were monitored over a two-year period. padsbrs significantly decreased the concentration of coliforms, s ... | 2011 | 20801022 |
| preterm birth and necrotizing enterocolitis alter gut colonization in pigs. | necrotizing enterocolitis (nec) in preterm neonates is dependent on bacterial colonization, but it remains unclear whether a particular microbiota or specific pathogens are involved. we hypothesized that gut colonization differs between preterm and term neonates and that overgrowth of clostridium perfringens predisposes to nec. by using terminal-rflp and fish, we characterized the gut microbiota of preterm, caesarean-delivered, formula-fed pigs (n = 44) with or without nec and of formula- or col ... | 2011 | 20924317 |
| clostridium perfringens sepsis and fetal demise after genetic amniocentesis. | clostridium perfringens is a rare cause of intrauterine infection. there have been five case reports concerning infection associated with invasive procedures. we report a woman who underwent a genetic amniocentesis due to her history of chronic granulomatous disease. she presented to the hospital ∼38 hours after the amniocentesis complaining of fever and chills. due to acute decompensation, she underwent an emergent dilatation and evacuation. during her stay, blood cultures came back positive fo ... | 2011 | 23705080 |
| fulminating septic shock from clostridium perfringens in an early breast cancer patient with severe myalgia after docetaxel treatment. | anaerobic bacteraemia could be a life-threatening condition in neutropenic patients receiving chemotherapy. taxane therapy is associated with necrotising inflammation of the caecum (named also typhlitis) that could be a potential source for bacteraemia. we report the case of a sudden onset of septic shock by clostridium perfringens in a young patient treated with docetaxel as adjuvant chemotherapy for early breast cancer. a mini-review of the literature has been performed. | 2011 | 24765295 |
| quality of water sources used as drinking water in a brazilian peri-urban area. | the objective of this paper was to assess bacteriological quality of drinking water in a peri-urban area located in the metropolitan region of são paulo, brazil. a total of 89 water samples were collected from community plastic tanks and 177 water samples from wells were collected bimonthly, from september 2007 to november 2008, for evaluating bacteriological parameters including: escherichia coli, enterococcus and heterotrophic plate count (hpc). clostridium perfringens was investigated in a su ... | 2011 | 24031668 |
| gas gangrene as a result of femoral traction pin placement. | treatment of adult femoral shaft fractures typically involves operative stabilization with intramedullary implants, external fixation, or a plate and screw construct. however, when stabilization is delayed for any reason, use of a traction pin is recommended to stabilize the fracture, prevent significant shortening, as well as to help with pain control. in this paper, we present the rare complication of a severe gas gangrene infection caused by clostridium perfringens that led to several amputat ... | 2011 | 23198212 |
| severe clostridial pyomyoma following an abortion does not always require surgical intervention. | background. clostridial infection following pregnancy may be fatal, and surgery is considered as the treatment of choice. we suggest a conservative management in selected cases when preservation of fertility is of major importance. case. a 41-year-old primigravida presented with abdominal pain and fever, one day following dilatation and curettage at 20 weeks of gestation. her abdomen was diffusely tender, with a uterus enlarged to 20 weeks' gestation. laboratory studies were consistent with seps ... | 2011 | 22567505 |
| impact of fresh or used litter on the posthatch immune system of commercial broilers. | this study was carried out to investigate the effects of exposure of growing broiler chickens of commercial origin to used poultry litter on intestinal and systemic immune responses. the litter types evaluated were fresh wood shavings or used litter obtained from commercial poultry farms with or without a history of gangrenous dermatitis (gd). immune parameters measured were serum nitric oxide (no) levels, serum antibody titers against eimeria or clostridium perfringens, mitogen-induced spleen c ... | 2011 | 22312971 |
| responses of feeding prebiotics on nutrient digestibility, faecal microbiota composition and short-chain fatty acid concentrations in dogs: a meta-analysis. | the effects of prebiotics on digestibility, short-chain fatty acid (scfa) concentrations and bacterial populations in the faeces and immunity in dogs were evaluated by meta-analyses. overall, data from 15 published studies containing 65 different treatment means of 418 observations from different breeds of dogs were included in the data set. feeding of prebiotics to dogs did not affect the nutrient intake (p > 0.10), nor did prebiotics change (p > 0.10) the digestibility of dry matter (dm) and f ... | 2011 | 22440414 |
| effect of temperature on microbial growth rate-mathematical analysis: the arrhenius and eyring-polanyi connections. | the objective of this work is to develop a mathematical model for evaluating the effect of temperature on the rate of microbial growth. the new mathematical model is derived by combination and modification of the arrhenius equation and the eyring-polanyi transition theory. the new model, suitable for both suboptimal and the entire growth temperature ranges, was validated using a collection of 23 selected temperature-growth rate curves belonging to 5 groups of microorganisms, including pseudomona ... | 2011 | 22417589 |
| claudin and ovarian cancer. | claudins are a family of proteins and the most important component of the tight junction. they constitute a paracellular barrier that controls the flow of molecules in the intercellular space of an epithelium. although it seems that claudin should be down regulated in cancer cell, some claudins are, in fact highly elevated in various human cancers, including ovarian cancer. whereas the functional significance of claudin overexpression in ovarian carcinoma is unclear, these proteins are important ... | 2010 | 24591894 |
| clostridium perfringens toxins involved in mammalian veterinary diseases. | clostridium perfringens is a gram-positive anaerobic rod that is classified into 5 toxinotypes (a, b, c, d, and e) according to the production of 4 major toxins, namely alpha (cpa), beta (cpb), epsilon (etx) and iota (itx). however, this microorganism can produce up to 16 toxins in various combinations, including lethal toxins such as perfringolysin o (pfo), enterotoxin (cpe), and beta2 toxin (cpb2). most diseases caused by this microorganism are mediated by one or more of these toxins. the role ... | 2010 | 24511335 |
| development and characterization of protective haemophilus parasuis subunit vaccines based on native proteins with affinity to porcine transferrin and comparison with other subunit and commercial vaccines. | haemophilus parasuis is the agent responsible for causing glässer's disease, which is characterized by fibrinous polyserositis, polyarthritis, and meningitis in pigs. in this study, we have characterized native outer membrane proteins with affinity to porcine transferrin (npapt) from h. parasuis serovar 5, nagasaki strain. this pool of proteins was used as antigen to developed two vaccine formulations: one was adjuvanted with a mineral oil (montanide ims 2215 vg pr), while the other was potentia ... | 2010 | 20926701 |
| a comparative in vitro investigation into the effects of cooked meats on the human faecal microbiota. | protein fermentation is one of the important microbial activities in the human colon. meat foods rich in protein provide substantial resource for this metabolic activity. however, little information exists on the relative impact of different meats on the composition and activities of the human gut microbiota. similarly, little information is available on the confounding effects of cooking on these activities. in this study, beef, chicken and fish (salmon) were examined in vitro for their impact ... | 2010 | 20934523 |
| clostridium perfringens alpha toxin is produced in the intestines of broiler chicks inoculated with an alpha toxin mutant. | poultry necrotic enteritis (ne) is caused by specific strains of clostridium perfringens, most of which are type a. the role of alpha toxin (cpa) in ne has been called into question by the finding that an engineered cpa mutant retains full virulence in vivo[9]. this is in contrast to the finding that immunization with cpa toxoids protects against ne. we confirmed the earlier findings, in that 14-day-old cornish × rock broiler chicks challenged with a cpa mutant developed lesions compatible with ... | 2010 | 20934524 |
| high-level production and purification of clostripain expressed in a virulence-attenuated strain of clostridium perfringens. | clostripain (clo) produced by clostridium histolyticum is an arginine-specific endopeptidase with the potential for applicability to diverse medical and industrial uses. in this study, we developed an expression system allowing high-level production and efficient purification of recombinant clo (rclo). our expression system comprises pclo, an rclo expressing vector, and clostridium perfringens 13δ6, an in-frame deletion strain as to six genes encoding major virulence factors and secretory protei ... | 2010 | 20940055 |
| clostridium perfringens epsilon toxin targets granule cells in the mouse cerebellum and stimulates glutamate release. | epsilon toxin (et) produced by c. perfringens types b and d is a highly potent pore-forming toxin. et-intoxicated animals express severe neurological disorders that are thought to result from the formation of vasogenic brain edemas and indirect neuronal excitotoxicity. the cerebellum is a predilection site for et damage. et has been proposed to bind to glial cells such as astrocytes and oligodendrocytes. however, the possibility that et binds and attacks the neurons remains an open question. usi ... | 2010 | 20941361 |
| comparison of a nontoxic variant of clostridium perfringens α-toxin with the toxic wild-type strain. | the α-toxin produced by clostridium perfringens is one of the best-studied examples of a toxic phospholipase c. in this study, a nontoxic mutant protein from c. perfringens strain nctc8237 in which thr74 is substituted by isoleucine (t74i) has been characterized and is compared with the toxic wild-type protein. thr74 is part of an exposed loop at the proposed membrane-interfacing surface of the toxin. the mutant protein had markedly reduced cytotoxic and myotoxic activities. however, this substi ... | 2010 | 20944240 |
| optimized necrotic enteritis model producing clinical and subclinical infection of clostridium perfringens in broiler chickens. | in this study we assessed the roles of eimeria infection and dietary manipulation (feeding a diet with a high level of fishmeal) in an australian necrotic enteritis (ne) challenge model in broiler chickens. an experiment was designed to test the hypothesis that eimeria infection and dietary manipulation, i.e., inclusion of fishmeal in the diet, are necessary to induce ne experimentally. the results showed that the combination of eimeria administration and fishmeal feeding had a significant effec ... | 2010 | 20945788 |
| probiotic properties of lactobacillus strains isolated from the feces of breast-fed infants and taiwanese pickled cabbage. | this study assessed potential probiotic lactobacillus strains isolated from the feces of breast-fed infants and from taiwanese pickled cabbage for their possible use in probiotic fermented foods by evaluating their (i) in vitro adhesive ability, resistance to biotic stress, resistance to pathogenic bacteria, and production of β-galactosidase; (ii) milk technological properties; and (iii) in vivo adhesive ability, intestinal survival and microbial changes during and after treatment. five lactobac ... | 2010 | 20951815 |
| evaluation of the efficacy of yeast extract in reducing intestinal clostridium perfringens levels in broiler chickens. | the etiological agent of necrotic enteritis is clostridium perfringens. traditionally, necrotic enteritis is controlled with in-feed antibiotics. however, increasing consumer demand for drug-free poultry has fostered the search for nonantibiotic alternatives. yeast extract contain nucleotides that are immunomodulatory and also essential for cellular functions. an experiment was conducted to evaluate the efficacy of nupro yeast extract (alltech inc., nicholasville, ky) in reducing intestinal c. p ... | 2010 | 20952700 |
| efficacy of a lactylate on production performance and intestinal health of broilers during a subclinical clostridium perfringens infection. | clostridium perfringens, an α-toxin producing gram-positive bacterium, is an enteric pathogen for poultry. because subclinical c. perfringens infections often result in damage of the intestinal mucosa, decreased nutrient digestion, and poor performance, efforts should be taken to find an effective strategy that controls overgrowth of c. perfringens. for this purpose, the efficacy of a sodium lauroyl lactylate (laul) as a feed additive to prevent c. perfringens colonization in broilers was determ ... | 2010 | 20952703 |
| clostridium perfringens bacteriophages φcp39o and φcp26f: genomic organization and proteomic analysis of the virions. | poultry intestinal material, sewage and poultry processing drainage water were screened for virulent clostridium perfringens bacteriophages. viruses isolated from broiler chicken offal washes (o) and poultry feces (f), designated φcp39o and φcp26f, respectively, produced clear plaques on host strains. both bacteriophages had isometric heads of 57 nm in diameter with 100-nm non-contractile tails characteristic of members of the family siphoviridae in the order caudovirales. the double-strand dna ... | 2010 | 20963614 |
| diversity and antibiotic susceptibility pattern of cultivable anaerobic bacteria from soil and sewage samples of india. | soil and sewage act as a reservoir of animal pathogens and their dissemination to animals profoundly affects the safety of our food supply. moreover, acquisition and further spread of antibiotic resistance determinants among pathogenic bacterial populations is the most relevant problem for the treatment of infectious diseases. bacterial strains from soil and sewage are a potential reservoir for antimicrobial resistance genes. accurate species determination for anaerobes from environmental sample ... | 2010 | 20965279 |
| molecular typing and antimicrobial susceptibility of clostridium perfringens from broiler chickens. | clostridium perfringens (cp) causes necrotic enteritis disease in commercial poultry. antimicrobials are used to control and treat this disease and sometimes clinical outbreaks do not respond well to certain treatments. this study was designed to isolate cp from clinical cases, type these isolates by multiplex pcr, and determine their antimicrobial susceptibility by micro-dilution method. a total of 67 cp isolates were obtained from 155 broiler chicken flocks. all isolates were classified as typ ... | 2010 | 20969969 |
| identification and modeling of a drug target for clostridium perfringens sm101. | in the present study, comparative genome analysis between clostridium perfringens and the human genome was carried out to identify genes that are essential for the pathogen's survival, and non-homologous to the genes of human host, that can be used as potential drug targets. the study resulted in the identification of 426 such genes. the number of these potential drug targets thus identified is significantly lower than the genome's protein coding capacity (2558 protein coding genes). the 426 gen ... | 2010 | 20978600 |
| food- and waterborne disease outbreaks in australian long-term care facilities, 2001-2008. | abstract food- or waterborne diseases in long-term care facilities (ltcf) can result in serious outcomes, including deaths, and they are potentially preventable. we analyzed data collected by ozfoodnet on food- and waterborne disease outbreaks occurring in ltcf in australia from 2001 to 2008. we compared outbreaks by the number of persons affected, etiology, and implicated vehicle. during 8 years of surveillance, 5.9% (55/936) of all food- and waterborne outbreaks in australia occurred in ltcf. ... | 2010 | 21034268 |
| ethanolamine utilization contributes to proliferation of salmonella enterica serovar typhimurium in food and in nematodes. | only three pathogenic bacterial species, salmonella enterica, clostridium perfringens, and listeria monocytogenes, are able to utilize both ethanolamine and 1,2-propanediol as a sole carbon source. degradation of these substrates, abundant in food and the gut, depends on cobalamin, which is synthesized de novo only under anaerobic conditions. although the eut, pdu, and cob-cbi gene clusters comprise 40 kb, the conditions under which they confer a selection advantage on these food-borne pathogens ... | 2010 | 21037291 |
| a case of clostridium perfringens infection in uterine sarcoma. | 2010 | 21039388 | |
| rapidly measured indicators of recreational water quality and swimming-associated illness at marine beaches: a prospective cohort study. | in the united states and elsewhere, recreational water quality is monitored for fecal indicator bacteria to help prevent swimming-associated illnesses. standard methods to measure these bacteria take at least 24 hours to obtain results. molecular approaches such as quantitative polymerase chain reaction (qpcr) can estimate these bacteria faster, in under 3 hours. previously, we demonstrated that measurements of the fecal indicator bacteria enterococcus using qpcr were associated with gastrointes ... | 2010 | 21040526 |
| comparison of selected nutrients and bacteria from common contiguous soils inside and outside swine lagoon effluent spray fields after long-term use. | swine (sus scofa domestica) lagoon effluent is a valuable resource. in the u.s. mid-south it is applied from april to september to fertilize grass hay in spray-irrigated fields. lagoon levels of nutrients and bacteria, and soil levels of nutrients have been documented, but little was known of effluent bacterial levels in soil. the present study examined levels of selected effluent bacteria and nutrients in soils inside and outside spray fields after >15 yr of effluent irrigation. samples were co ... | 2010 | 21043289 |
| clostridium perfringens: a flesh-eating bacterium living in your garden. | gas gangrene is a painful, rapidly developing and potentially fatal infection despite antibiotic treatment. during the first world war thousands of soldiers died from this disease. dr alexis carrel pioneered a controversial method of irrigating wounds with dakin's solution to destroy clostridium perfringens, a bacterium found in heavily fertilised soils that causes gas gangrene. although this method is no longer used due to the discovery of antibiotics, many of his other ideas, such as scientifi ... | 2010 | 21049805 |
| a novel glycosylation signal regulates transforming growth factor beta receptors as evidenced by endo-beta-galactosidase c expression in rodent cells. | the αgal (galα1-3gal) epitope is a xenoantigen that is responsible for hyperacute rejection in xenotransplantation. this epitope is expressed on the cell surface in the cells of all mammals except humans and old world monkeys. it can be digested by the enzyme endo-β-galactosidase c (endogalc), which is derived from clostridium perfringens. previously, we produced endogalc transgenic mice to identify the phenotypes that would be induced following endogalc overexpression. the mice lacked the αgal ... | 2010 | 21062784 |
| effect of laser and led on enzymatic production of ceramide. | an enzyme (phospholipase c type i from clostridium perfringens) was exposed to 0-810jcm(-2) of energy using laser light at wavelengths 808, 532, 1064 and 1342nm and two led light sources at wavelengths 810 and 640nm. enzyme responses were evaluated by measuring ceramide concentration using high performance thin-layer chromatography (hptlc) at 0.5, 1, 2, 3, 4, 6, 17, 24h after irradiation. the duration of effect was evaluated from the experimental data. the results show that enzyme activity can b ... | 2010 | 21073471 |
| nanoparticle probes and mid-infrared chemical imaging for dna microarray detection. | to date most mid-infrared spectroscopic studies have been limited, due to lack of sensitivity, to the structural characterization of a single oligonucleotide probe immobilized over the entire surface of a gold-coated slide or other infrared substrate. by contrast, widely used and commercially available glass slides and a microarray spotter that prints approximately 120-μm-diameter dna spots were employed in the present work. to our knowledge, mid-infrared chemical imaging (irci) in the external ... | 2010 | 21073786 |
| tangential-flow ultrafiltration with integrated inhibition detection for recovery of surrogates and human pathogens from large-volume source water and finished drinking water. | tangential-flow ultrafiltration was optimized for the recovery of escherichia coli, enterococcus faecalis, clostridium perfringens spores, bacteriophages ms2 and prd1, murine norovirus, and poliovirus seeded into 100-liter surface water (sw) and drinking water (dw) samples. sw and dw collected from two drinking water treatment plants were then evaluated for human enteric viruses. | 2010 | 21075885 |
| companion animals symposium: microbes and gastrointestinal health of dogs and cats. | recent molecular studies have revealed complex bacterial, fungal, archaeal, and viral communities in the gastrointestinal tract of dogs and cats. more than 10 bacterial phyla have been identified, with firmicutes, bacteroidetes, proteobacteria, fusobacteria, and actinobacteria constituting more than 99% of all gut microbiota. microbes act as a defending barrier against invading pathogens, aid in digestion, provide nutritional support for enterocytes, and play a crucial role in the development of ... | 2010 | 21075970 |
| effect of the cortex-lytic enzyme slec from non-food-borne clostridium perfringens on the germination properties of slec-lacking spores of a food poisoning isolate. | the hallmark of bacterial spore germination is peptidoglycan cortex hydrolysis by cortex-lytic enzymes. in spores of clostridium perfringens wild-type strain sm101, which causes food poisoning, the sole essential cortex-lytic enzyme slec is activated by a unique serine protease cspb. interestingly, the non-food-borne wild-type strain f4969 encodes a significantly divergent slec variant (slecf4969) and 3 serine proteases (cspa, cspb, and cspc). consequently, in this study we evaluated the functio ... | 2010 | 21076486 |
| microbiological quality of blue mussels (mytilus edulis) in nunavik, quebec: a pilot study. | this pilot study was aimed at documenting the presence of fecal indicators and enteric pathogens in blue mussels (mytilus edulis) from 6 communities in nunavik, quebec. one to four 2 kg samples of mussels were collected at low tide in each community. samples were investigated by enumeration methods for the fecal indicators enterococci, escherichia coli, f-specific coliphages, clostridium perfringens, and by molecular identification for the pathogens norovirus, salmonella spp., campylobacter jeju ... | 2010 | 21076488 |
| perfringolysin o association with ordered lipid domains: implications for transmembrane protein raft affinity. | upon interaction with cholesterol, perfringolysin o (pfo) inserts into membranes and forms a rigid transmembrane (tm) β-barrel. pfo is believed to interact with liquid ordered lipid domains (lipid rafts). because the origin of tm protein affinity for rafts is poorly understood, we investigated pfo raft affinity in vesicles having coexisting ordered and disordered lipid domains. fluorescence resonance energy transfer (fret) from pfo trp to domain-localized acceptors indicated that pfo generally h ... | 2010 | 21081073 |
| lytic enzyme discovery through multigenomic sequence analysis in clostridium perfringens. | with their ability to lyse gram-positive bacteria, phage lytic enzymes (or lysins) have received a great deal of attention as novel anti-infective agents. the number of known genes encoding these peptidoglycan hydrolases has increased markedly in recent years, due in large part to advances in dna sequencing technology. as the genomes of more and more bacterial species/strains are sequenced, lysin-encoding open reading frames (orfs) can be readily identified in lysogenized prophage regions. in th ... | 2010 | 21085950 |
| in vivo studies of clostridium perfringens in mouse gas gangrene model. | understanding the pathogenesis of infectious diseases requires comprehensive knowledge of the proteins expressed by the pathogen during in vivo growth in the host. proteomics provides the tools for such analyses but the protocols required to purify sufficient quantities of the pathogen from the host organism are currently lacking. in this study, we have separated clostridium perfringens, a highly virulent bacterium and potential btw agent, from the peritoneal fluid of infected mice using percoll ... | 2010 | 21086128 |
| estimation of the environmental risk posed by landfills using chemical, microbiological and ecotoxicological testing of leachates. | the leachates from 22 municipal solid waste (msw) landfill sites in southern poland were characterized by evaluation of chemical, microbiological and ecotoxicological parameters. chemical analyses were mainly focused on the identification of the priority hazardous substances according to directive on priority substances, 2008/105/ec (a daughter directive of the wfd) in leachates. as showed, only five substances (cd, hg, hexachlorobutadiene, pentachlorobenzene and pahs) were detected in the leach ... | 2010 | 21087786 |
| clinical and epidemiological features of clostridium perfringens bacteremia: a review of 18 cases over 8 year-period in a tertiary care center in metropolitan tokyo area in japan. | clostridial sepsis has a very poor prognosis, owing to the life-threatening combination of shock and acute massive hemolysis. no papers have described the clinical features of clostridial sepsis cases in japan. therefore, we retrospectively examined the clinical features of patients with systemic inflammatory response syndrome (sirs) from whose blood cultures clostridium perfringens was isolated. | 2010 | 21088344 |
| construction and characterization of a lactose-inducible promoter system for controlled gene expression in clostridium perfringens. | clostridium perfringens is a gram-positive anaerobic pathogen which causes many diseases in humans and animals. while some genetic tools exist for working with c. perfringens, a tightly regulated, inducible promoter system is currently lacking. therefore, we constructed a plasmid-based promoter system that provided regulated expression when lactose was added. this plasmid (pkrah1) is an escherichia coli-c. perfringens shuttle vector containing the gene encoding a transcriptional regulator, bgar, ... | 2010 | 21097603 |
| clostridium perfringens tpel glycosylates the rac and ras subfamily proteins. | clostridium perfringens tpel belongs to a family of large clostridial cytotoxins that encompasses clostridium difficile toxin a (tcda) and b (tcdb) and clostridium sordellii lethal toxin (tcsl). we report here the identification of the tpel-catalyzed modification of small gtpases. a recombinant protein (tpel1-525) derived from the tpel n-terminal catalytic domain in the presence of streptolysin o (slo) induced the rounding of vero cells and the glycosylation of cellular rac1. among several hexos ... | 2010 | 21098103 |
| the safety of a mucosal vaccine using the c-terminal fragment of clostridium perfringens enterotoxin. | the c-terminal fragment of clostridium perfringens enterotoxin (c-cpe) is a claudin-4 binder. very recently, we found that nasal immunization of mice with c-cpe-fused antigen activated antigen-specific humoral and mucosal immune responses and that the deletion of the claudin-4-binding domain attenuated the immune responses. c-cpe-fusion strategy may be useful for mucosal vaccination. c-cpe is a fragment of enterotoxin, and the safety of c-cpe-fused protein is very important for its future applic ... | 2010 | 21105580 |
| germination of spores of bacillales and clostridiales species: mechanisms and proteins involved. | under conditions that are not conducive to growth, such as nutrient depletion, many members of the orders bacillales and clostridiales can sporulate, generating dormant and resistant spores that can survive in the absence of nutrients for years under harsh conditions. however, when nutrients are again present, these spores can return to active growth through the process of germination. many of the components of the spore germination machinery are conserved between spore forming members of the ba ... | 2010 | 21112786 |
| [intestinal microflora of autistic children]. | autistic behavior is often accompanied by numerous disturbing symptoms on the part of gastrointestinal system, such as abdominal pain, constipation or diarrhea. these problems are often connected with deregulation of physiological microflora in intestine. the aim of this study was to determine differences in intestinal microflora of autistic and healthy children. strains of clostridium spp. and enterococci were isolated more frequently from stool samples of autistic children and rarely lactobaci ... | 2010 | 21114016 |
| fatal clostridial necrotising myofasciitis (gas gangrene) following femoral nerve block. | 2010 | 21114743 | |
| c terminus of clostridium perfringens enterotoxin downregulates cldn4 and sensitizes ovarian cancer cells to taxol and carboplatin. | we have previously shown that cldn4 (encoding claudin-4), a cell tight junction (tj) protein, is highly expressed in human epithelial ovarian carcinomas (eoc) but undetectable in normal ovaries. cldn4 has been identified as a specific receptor for c terminus of clostridium perfringens enterotoxin (c-cpe), a nontoxic molecule that may disrupt tj barrier function and enhance cellular absorption. the purpose of this study was to determine the potential clinical applications of c-cpe and its effects ... | 2010 | 21123456 |
| antimicrobial activity of satureja montana l. essential oil against clostridium perfringens type a inoculated in mortadella-type sausages formulated with different levels of sodium nitrite. | this research evaluated the antimicrobial effect of the winter savory (satureja montana l.) essential oil (eo) against clostridium perfringens type a (atcc 3624) inoculated in mortadella-type sausages formulated with different levels of sodium nitrite (nano₂: 0 ppm, 100 ppm and 200 ppm) in addition to eo at concentrations of 0.0%, 0.78%, 1.56% and 3.125% stored at 25°c for 30 days. the eo extracted by hydrodistillation and analyzed by gas chromatography-mass spectrometry (cg-ms) was tested in vi ... | 2010 | 21131083 |
| focal symmetrical encephalomalacia in a goat. | focal symmetrical encephalomalacia (fse) is the most prominent lesion seen in the chronic form of enterotoxemia caused by clostridium perfringens type d in sheep. however, this lesion has not been reported in goats. the current paper reports a case of fse in a goat from the state of paraíba in the brazilian semiarid region. as reported by the farmer, 30, 4-48-month-old animals from a flock of 150 goats died after showing nervous signs, including blindness and recumbence, for periods varying betw ... | 2010 | 20807946 |
| production of genetically modified porcine blastocysts by somatic cell nuclear transfer: preliminary results toward production of xenograft-competent miniature pigs. | galα1-3gal (α-gal epitope) is the major xenoantigenic epitope responsible for hyperacute rejection upon pig-to-human xenotransplantation. endo-β-galactosidase c (endogalc) from clostridium perfringens can digest the α-gal epitope. in this study, gene-engineered primary cultured porcine embryonic fibroblasts (pef) expressing endogalc were obtained and subjected to somatic cell nuclear transfer (scnt) to test whether xenograft-competent pigs can be created. the endogalc-expressing pef clones exhib ... | 2010 | 20814171 |
| peptoniphilus methioninivorax sp. nov., a novel gram-positive anaerobic coccus isolated from retail ground beef. | strain nrrl b-23883 was isolated from retail ground beef as part of a study on the genetic diversity of clostridium perfringens. the strain was found to be a strictly anaerobic, gram- positive coccus that was able to utilize peptone as a sole carbon source. subsequent to sequencing the 16s ribosomal rna gene, it was found that the strain was closely related to species within the genera peptoniphilus and anaerosphaera, but it was substantially different from the closest recognized species by near ... | 2010 | 20817843 |
| global regulation of gene expression in response to cysteine availability in clostridium perfringens. | cysteine has a crucial role in cellular physiology and its synthesis is tightly controlled due to its reactivity. however, little is known about the sulfur metabolism and its regulation in clostridia compared with other firmicutes. in clostridium perfringens, the two-component system, virr/virs, controls the expression of the ubig operon involved in methionine to cysteine conversion in addition to the expression of several toxin genes. the existence of links between the c. perfringens virulence ... | 2010 | 20822510 |
| oga inhibition by glcnac-selenazoline. | the title compound, which differs from the powerful o-glcnacase (oga) inhibitor glcnac-thiazoline only at the chalcogen atom (se for s), is a much weaker inhibitor in a direct oga assay. in human cells, however, the selenazoline shows comparable ability to induce hyper-o-glcnac-ylation, and the two show similar reduction of insulin-stimulated translocation of glucose transporter 4 in differentiated 3t3 adipocytes. | 2010 | 20822912 |
| characterization of toxin plasmids in clostridium perfringens type c isolates. | clostridium perfringens type c isolates cause enteritis necroticans in humans or necrotizing enteritis and enterotoxemia in domestic animals. type c isolates always produce alpha toxin and beta toxin but often produce additional toxins, e.g., beta2 toxin or enterotoxin. since plasmid carriage of toxin-encoding genes has not been systematically investigated for type c isolates, the current study used southern blot hybridization of pulsed-field gels to test whether several toxin genes are plasmid ... | 2010 | 20823204 |
| recombinant expression of two bacteriophage proteins that lyse clostridium perfringens and share identical sequences in the c-terminal cell wall binding domain of the molecules but are dissimilar in their n-terminal active domains. | clostridium perfringens is a gram-positive anaerobic spore-forming bacterium capable of producing four major toxins that are responsible for disease symptoms and pathogenesis in a variety of animals, humans, and poultry. the organism is the third leading cause of human foodborne bacterial disease, and c. perfringens is the presumptive etiologic agent of necrotic enteritis among chickens, which in the acute form can cause increased mortality among broiler flocks. countries that have complied with ... | 2010 | 20825156 |
| quantitative in vitro assay to evaluate the capability of yeast cell wall fractions from trichosporon mycotoxinivorans to selectively bind gram negative pathogens. | yeast cell wall fractions have been proposed to bind enteropathogenic bacteria. the aim of this study was to develop a quantitative assay by measuring the optical density as growth parameter of adhering bacteria. the exponential growth phase of adhering bacteria was determined by optical density reading and compared with the colony count (cfu/ml). a linear regression was compiled and the bacterial number bound to the yeast cell wall product could be determined. further focus was the investigatio ... | 2010 | 20826190 |
| novel use of tryptose sulfite cycloserine egg yolk agar for isolation of clostridium perfringens during an outbreak of necrotizing enterocolitis in a neonatal unit. | clostridium perfringens has been associated with necrotizing enterocolitis (nec), which is a serious disease of neonates. our study describes the novel use of selective tryptose sulfite cycloserine with egg yolk agar (tsc-eya) during a nursery outbreak. this medium provides a rapid, sensitive, and accurate presumptive identification of c. perfringens. | 2010 | 20826643 |
| prevalence of clostridium perfringens in faeces and ileal contents from grass sickness affected horses: comparisons with 3 control populations. | while previous studies have demonstrated an association between equine grass sickness (egs) and the presence of clostridium botulinum within ileal contents and faeces, no such associations with other intestinal-derived anaerobic bacteria have been extensively investigated. | 2010 | 20716188 |
| rapid detection of enterotoxigenic clostridium perfringens in meat samples using immunomagnetic separation polymerase chain reaction (ims-pcr). | rapid detection of enterotoxigenic clostridium perfringens in meat samples was accomplished with an immunomagnetic separation polymerase chain reaction (ims-pcr). first, a monoclonal antibody (mab) specific to c. perfringens was generated. the antibody showed strong binding to c. perfringens and no binding to non- clostridia bacteria, except a weak cross-reaction to staphylococcus aureus based on the enzyme-linked immunosorbent assay (elisa). then, magnetic beads were coated with the mab, and th ... | 2010 | 20507064 |
| identification of small molecule inhibitors of clostridium perfringens ε-toxin cytotoxicity using a cell-based high-throughput screen. | the clostridium perfringens epsilon toxin, a select agent, is responsible for a severe, often fatal enterotoxemia characterized by edema in the heart, lungs, kidney, and brain. the toxin is believed to be an oligomeric pore-forming toxin. currently, there is no effective therapy for countering the cytotoxic activity of the toxin in exposed individuals. using a robust cell-based high-throughput screening (hts) assay, we screened a 151,616-compound library for the ability to inhibit ε-toxin-induce ... | 2010 | 20721308 |
| catchment process affecting drinking water quality, including the significance of rainfall events, using factor analysis and event mean concentrations. | to ensure the protection of drinking water an understanding of the catchment processes which can affect water quality is important as it enables targeted catchment management actions to be implemented. in this study factor analysis (fa) and comparing event mean concentrations (emcs) with baseline values were techniques used to asses the relationships between water quality parameters and linking those parameters to processes within an agricultural drinking water catchment. fa found that 55% of th ... | 2010 | 20705986 |
| an outbreak of gangrenous dermatitis in commercial broiler chickens. | the present report describes an outbreak of gangrenous dermatitis (gd) infection in a commercial poultry farm in delaware involving 34-day-old broiler chickens. in addition to obvious clinical signs, some gd-affected broilers also showed severe fibrino-necrotic enteritis and large numbers of gram-positive rods in the necrotic tissue. histopathological findings included haemorrhage, degeneration and necrosis of parenchymatous cells, especially of skin, muscle, and intestine. immunofluorescence st ... | 2010 | 20706880 |
| immunopathology and cytokine responses in commercial broiler chickens with gangrenous dermatitis. | gangrenous dermatitis (gd) is an emerging disease of increasing economic importance in poultry resulting from infection by clostridium septicum and clostridium perfringens type a. lack of a reproducible disease model has been a major obstacle in understanding the immunopathology of gd. to gain better understanding of host-pathogen interactions in gd infection, we evaluated various immune parameters in two groups of birds from a recent commercial outbreak of gd, the first showing typical disease ... | 2010 | 20706881 |
| modified toxin-binding inhibition (tobi) test for epsilon antitoxin determination in serum of immunized rabbits. | the aim of the present study was to evaluate and standardize the tobi test in vitro as a substitute for the serum neutralization test in mice for quality control of clostridial vaccines. the tobi test in vitro was used to evaluate 40 serum samples of known antibody content, obtained from rabbits immunized against clostridiosis with experimental vaccine. the correlation between epsilon antitoxin titers in rabbit sera, determined by the tobi test and serum neutralization in mice, ranged from 0.222 ... | 2010 | 20709411 |
| effects of depletion of rna-binding protein tex on the expression of toxin genes in clostridium perfringens. | tex was originally identified in bordetella pertussis, where it serves as a transcriptional regulator of toxin genes. however, the tex of streptococcus pneumoniae has no regulatory function in the expression of the pneumococcal major toxin pneumolysin. here, we identified the cpe2168 gene as tex in clostridium perfringens, and examined the roles of tex in toxin gene expression. we found that the deletion mutant for tex does not affect growth, but the mrna levels of three hyaluronidase genes (nag ... | 2010 | 20699586 |
| effect of daylight on regrowth of bacteria in anaerobically digested sludge. | this study investigated the regrowth of total coliform, salmonella, and clostridium perfringens in anaerobically digested sludge after centrifuge dewatering in the presence and absence of daylight. sludge cake and centrate samples were collected from a treatment plant, and half of the samples was stored in daylight and the other half was stored in dark for three weeks. the bacteria levels in the cake and centrate samples were measured periodically throughout the storage period, and all three bac ... | 2010 | 20651441 |
| the ability of disease and non-disease producing strains of clostridium perfringens from chickens to adhere to extracellular matrix molecules and caco-2 cells. | clostridium perfringens is a major enteric pathogen that is responsible for causing necrotic enteritis of poultry. the ability to adhere to the host's intestinal epithelium and to extracellular matrix molecules (ecmm) in the gut, are strategies used by numerous bacterial enteropathogens, however, c. perfringens has received comparatively little attention in this respect. the present study investigated sixteen type a c. perfringens isolates from chickens, with varying disease producing ability wi ... | 2010 | 20654724 |
| polymer partitioning and ion selectivity suggest asymmetrical shape for the membrane pore formed by epsilon toxin. | using poly-(ethylene glycol)s of different molecular weights, we probe the channels formed in planar lipid bilayers by epsilon toxin secreted by the anaerobic bacterium clostridium perfringens. we find that the pore is highly asymmetric. the cutoff size of polymers entering the pore through its opening from the cis side, the side of toxin addition, is approximately 500 da, whereas the cutoff size for the polymers entering from the trans side is approximately 2300 da. comparing these characterist ... | 2010 | 20682255 |
| a 31-year-old man with liver transplant and progressive jaundice. | 2010 | 20682535 | |
| clostridium perfringens liver abscess with massive haemolysis. | liver abscesses are commonly caused by enterobacteriaceae and anaerobes. this report is of a patient with liver abscess with massive haemolysis and multiorgan failure caused by clostridium perfringens. despite the reportedly high mortality rate and poor prognostic factors, the patient eventually recovered with prompt treatment. | 2010 | 20683077 |
| laetiporus sulphureus lectin and aerolysin protein family. | the parasitic mushroom laetiporus sulphureus produces a family of lectins (lsl's) sharing 80-90% sequence identity that possesses a low but significant sequence similarity to the bacterial pore-forming toxins mosquitocidal toxin mtx-2 from bacillus sphaericus and a toxin from clostridium septicum. the crystal structure of one member of the l. sulphureus lectins family (lsla) reveals unexpected structural similarities to the 1-pore-forming toxins from the aerolysin family, namely, aerolysin from ... | 2010 | 20687481 |
| implications of faecal indicator bacteria for the microbiological assessment of roof-harvested rainwater quality in southeast queensland, australia. | the study aimed to evaluate the suitability of escherichia coli, enterococci, and clostridium perfringens for assessing the microbiological quality of roof-harvested rainwater and assessing whether the concentrations of these faecal indicators can be used to predict the presence or absence of specific zoonotic bacterial or protozoan pathogens. from a total of 100 samples tested, 58%, 83%, and 46% of samples were found to be positive for, respectively, e. coli, enterococci, and clostridium perfri ... | 2010 | 20657617 |
| phenotypic and genotypic characterization of tetracycline and minocycline resistance in clostridium perfringens. | the aim of this study was to determine the incidence of tetracycline resistance and the prevalence of tetracycline-resistance genes in strains of clostridium perfringens isolated from different sources between 1994 and 2005. susceptibility to tetracycline and minocycline in strains from humans (35 isolates), chickens (15 isolates), food (21 isolates), soil (16 isolates) and veterinary sources (6 isolates) was determined, and tetracycline-resistance genes were detected. resistance was most common ... | 2010 | 20661548 |