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bacterial vaginosis.bacterial vaginosis (bv) is the most common of the vaginitides affecting women of reproductive age. it appears to be due to an alteration in the vaginal ecology by which lactobacillus spp., the predominant organisms in the healthy vagina, are replaced by a mixed flora including prevotella bivia, prevotella disiens, porphyromonas spp., mobiluncus spp., and peptostreptococcus spp. all of these organisms except mobiluncus spp. are also members of the endogenous vaginal flora. while evidence from tr ...19911747864
microbiological study of hiv-related periodontitis.the subgingival microbiota in 14 persons with hiv-periodontitis was examined. subgingival plaque samples were collected with paper points, transported in vmga iii, and plated on anaerobic enriched brucella blood agar and various selective media. hiv-periodontitis sites revealed actinobacillus actinomycetemcomitans, wolinella recta, peptostreptococcus micros, and bacteroides intermedius, each averaging 7% to 16% of the cultivable subgingival flora in positive patients. high levels of spirochetes ...19912002434
metabolism of 1,4-dinitro-2-methylpyrrole, a mutagen formed by a sorbic acid-nitrite reaction, by intestinal bacteria.1,4-dinitro-2-methylpyrrole (dnmp), a mutagenic product formed by the interaction of two common food additives, sorbic acid and sodium nitrite, was transformed to 1-nitro-2-methyl-4-aminopyrrole (nmap) by human fecal mixtures and various intestinal bacterial strains. under anaerobic conditions the cell suspensions of actinomyces, bacteroides, clostridium, eubacterium, fusobacterium, and peptostreptococcus spp. demonstrated the nitroreduction activity. under aerobic conditions, only actinomyces a ...19912022195
microbiological studies of decubitus ulcers in children.specimens from 58 children with decubitus ulcers were cultured for aerobic and anaerobic bacteria. aerobic bacteria only were present in 29 (50%) ulcers, anaerobic bacteria only were recovered in 5 (9%), and mixed aerobic and anaerobic flora were present in 24 (41%). a total of 132 isolates (79 aerobes, 53 anaerobes) were recovered, an average of 2.3 isolates per specimen (1.4 aerobes, 0.9 anaerobes). the smallest number of isolates was recovered in ulcers of the skull (1.7 per site), and the hi ...19912023086
the microflora of periodontal sites showing active destructive progression.20 adult periodontitis (ap) subjects were examined every 2 to 4 months and microbiological samples were collected and cultured when 2 mm or more loss of attachment (active sites) was detected by 2 examiners. similar sites in which no progressive destruction was observed (control sites) also were sampled in the same subjects. by lambda-analysis, there was no statistically significant difference in floras of active (42 sites from 12 subjects) and control (36 sites from 12 subjects) sites or betwee ...19911752997
[chronic conjunctivitis caused by oral anaerobic germs].a patient with chronic, unilateral conjunctivitis is presented. the predominant bacterial flora consisted mainly of prevotella intermedia (formerly bacteroides intermedius) and peptostreptococcus micros. restriction endonuclease fingerprinting of genomic dna demonstrated that p. intermedia most probably originated from the oral cavity. in the oral cavity this species was recovered from the dorsum of the tongue, the tonsillar area and the periodontal pockets. topical treatment with various antibi ...19911758517
[a classic case of ludwig's angina].the aa. have the chance to review the etiology, the clinic and the management of this risky process, through a characteristic ludwig's angina of their own practice. a middle age man carrying an odontogenic inflammation developed a spreading infection into the deep neck tissues. the problem was successfully resolved with antibiotherapy and surgical drainage.19911781512
adhesion of 1-carbon utilizing bacteria to polymeric surfaces. 19912037198
[antibacterial activity of ciprofloxacin against fresh clinical isolates from superficial suppurative foci].the minimum inhibitory concentrations (mics) of 5 drugs (ciprofloxacin (cpfx), and 4 drugs used as standard) were determined to investigate antibacterial potencies of cpfx against bacterial strains isolated in 1989 from superficial suppurative foci. the clinical isolates tested included 375 strains from 11 aerobic bacterial species, and 50 strains from 2 anerobic bacterial genera (group) for a total of 425 isolates. interpreting mic level distributions of these drugs as the expression of antibac ...19912041153
vaginal bacteroides species are associated with an increased rate of preterm delivery among women in preterm labor.the relationship of high concentrations of vaginal microorganisms with an increased rate of preterm delivery (less than or equal to 34 weeks) among women in preterm labor was evaluated. quantitative vaginal cultures were obtained from 211 women in preterm labor between gestational ages of 22 and 34 completed weeks. preterm delivery occurred in 139 (66%). the rate of preterm delivery was determined by cox proportional hazards models. women with greater than 10(7) cfu/ml of vaginal fluid of facult ...19912056221
the differentiation of asaccharolytic anaerobic gram-positive cocci by protein electrophoresis.conventional biochemical and antibiotic sensitivity tests were used to allocate 87 clinical isolates of anaerobic gram-positive cocci to currently recognised species, in comparison with type and other authentic reference strains. whole-cell protein electrophoresis was then performed with extracts of each strain. allowing for difficulties of standardisation, it was possible to allocate most of the organisms to species-related groups on the basis of protein patterns. organisms identified conventio ...19912056518
[analysis of 98 cases of thoracic empyema].ninety-eight cases of empyema thoracis admitted to juntendo university hospital between 1979 and 1990 were reviewed. males accounted for 78 cases and females 20 cases. thirteen pediatric patients ranged in age from 17 days to 4 years, while the 85 adult cases ranged from 16 to 89 years (mean: 58.4 years). the mortality rate increased with age. fifty-three cases of community-acquired empyema thoracis consisted of 24 with no underlying disease (including 13 pediatric cases), and 29 with diabetes m ...19911783810
[effects of antibiotics and anti-bacterial components of preparations for local treatment of suppurative wounds on pathogens of odonto- genic infections].the results of identification and sensitivity assay of 156 strains of pathogens causing odontogenic infections are presented. in the sensitivity assay antibacterial drugs were used. 42.3 percent of the strains were obligate anaerobes belonging to bacteroides, fusobacterium, peptococcus, peptostreptococcus, veillonella and actinomyces. significant differences in the microbial sensitivity to the drugs used for general and local therapy were detected. there was observed high sensitivity of the obli ...19911805697
[empyema due to an anaerobic streptococcus in childhood].anaerobic infections are quite rare in pediatric age, being that, they affect only neonates and immunodepressed patients. we think to be somewhat interesting to describe the case of our patient, a 9 year old boy, unaffected by any predisposing factor, came under our observation because of a severe respiratory distress. he showed evident clinical and radiological signs of pleural effusion in the right lung, together with a gas coil in the upper field and a left mediastinal shifting. a thoracentes ...19911806927
predominant cultivable flora in pericoronitis.the predominant cultivable flora in pericoronitis was investigated by culturing pus from affected sites in 20 patients. twenty colonies were picked at random from non-selective plates and identified using conventional biochemical and physiological tests, analysis of metabolic end-products by gas chromatography and protein profile analysis. the most frequently isolated organisms were prevotella (bacteroides) intermedia, peptostreptococcus micros, veillonella species, fusobacterium nucleatum and s ...19911820571
native valve infective endocarditis in adults--analysis of 32 consecutive cases over a ten-year period from 1980 to 1989.thirty-two patients with native valve infective endocarditis who presented over a 10-year period at our hospital were analyzed retrospectively. the presenting symptom was a persistent fever in 22 patients. in 30 patients, the new york heart association functional class was less than ii before the development of endocarditis. blood cultures were positive on all occasions in 24 out of 29 culture-positive patients. all of the viridans streptococci, accounting for 79% of the isolated pathogens, were ...19911829492
the importance of positive bacterial cultures of specimens obtained during clean orthopaedic operations.microbiological cultures of specimens of tissue and of fluids from the wound in forty patients who had had consecutive clean, elective orthopaedic operations (excluding total joint replacements) and had not received antibiotics preoperatively were analyzed. of the forty patients, twenty-three (58 per cent) had a positive culture on at least one of the media that were used and seventeen (43 per cent) had negative cultures. of the forty specimens that were obtained from swabbing of the wound, eigh ...19911890121
chronic conjunctivitis caused by oral anaerobes and effectively treated with systemic metronidazole plus amoxicillin.in this study, we report on a case of refractory, unilateral anaerobic conjunctivitis. the predominant anaerobic flora consisted of prevotella intermedia (formerly bacteroides intermedius) and peptostreptococcus micros. by using the technique of restriction endonuclease fingerprinting of genomic dna, it was shown that the p. intermedia likely originated from the oral cavity. topically applied antibiotics had failed to suppress the infection in the past. successful treatment was achieved after sy ...19911890173
[strict anaerobic bacteria: comparative study of various beta-lactam antibiotics in combination with tazobactam or sulbactam].the minimal inhibitory concentrations of piperacillin (pip) or cefotaxime (ctx) alone or in combination with tazobactam (taz) were determined against 168 anaerobes. all the strains were inhibited by pip + taz, but certain strains resistant to ctx + taz were found among b. fragilis, eubacterium and peptostreptococcus. the second investigations included 30 strains of bacteroides fragilis. concentrations of 2, 4 and 8 mg/l of taz and sulbactam (sul) were combined with piperacillin or cefotaxime. th ...19911652729
microbial aspects of failing osseointegrated dental implants in humans.the subgingival microflora of 18 failing implants were examined for pathogenic periodontal microorganisms. peptostreptococcus micros was recovered from 6 failing implants, wolinella recta from 6, fusobacterium species from 5, candida albicans from 5, and bacteroides intermedius from 4. enteric rods or pseudomonads constituted a significant part of the microflora in 5 failing implants. actinobacillus actinomycetemcomitans, nonpigmented bacteroides species, capnocytophage species, and staphylococc ...19911906537
[hemolytic jaundice in anaerobic bacteremia. a report of a case resolved surgically].the authors report a case of haemolytic jaundice caused by anaerobic bacteria arising from an abdominal abscess. this is undoubtedly a rare pathology but, when occurring, the resolution may be achieved by surgical therapy.19911911074
suitability of the broth-disk elution test for evaluating susceptibility of obligate anaerobes to trimethoprim-sulfonamides.species of anaerobic bacteria isolated from clinical veterinary specimens were tested for susceptibility to trimethoprim-sulfonamides by the broth-disk elution technique. three different media were used for each organism: prereduced anaerobically sterilized (pras) brain-heart infusion broth (bhi), thioglycollate broth, and a semidefined pras medium. susceptibility results from these media were compared with those determined by interpreting the minimal inhibitory concentration obtained using an a ...19911911991
nosocomial infections of ocular conjunctiva in newborns delivered by cesarian section.colonization of the ocular conjunctiva in newborns delivered by cesarian section occurs usually within the first day of life. we have studied the flora of the ocular conjunctiva at birth, from 19 newborns delivered by cesarian section, coming from two different maternity hospitals. ocular conjunctiva cultures yielded the main predominant flora in both maternity hospitals considered. the most common genus of this flora are: staphylococcus, corynebacterium and propionibacterium acnes. peptostrepto ...19911870845
[clinical study on cefteram pivoxil in asymptomatic bacteriospermia].in order to understand the clinical efficacy of cefteram pivoxil (cftm) in the treatment of asymptomatic bacteriospermia, the following studies were performed. 1. concentrations of cftm in the semen after oral administration of 200 mg to normal healthy adults (n = 5) reached a maximum level of 0.66 +/- 0.04 microgram/ml in 5 hours after the administration, then decreased rapidly, and averaged 0.15 +/- 0.03 micrograms at 7 hours after administration. 2. activities of cftm, cefaclor (ccl) and lome ...19911880933
anaerobic pathogenesis: collagenase production by peptostreptococcus magnus and its relationship to site of infection.fifty isolates of peptostreptococcus magnus from intraabdominal sepsis, nonpuerperal breast abscess, and diabetic foot infections were examined for collagenase activity using bovine type i collagen. collagenase production was detected in a higher percentage of strains from nonpuerperal breast and diabetic foot specimens (p less than .001). this enzyme may be responsible for p. magnus playing a more central role in the pathogenesis of nonpuerperal breast abscess and diabetic foot disease than in ...19911850443
microbial risk indicators for periodontal attachment loss. 19911831856
bacteria in the apical 5 mm of infected root canals.ten freshly extracted teeth which had carious pulpal exposures and periapical lesions contiguous with the root apex were placed inside an anaerobic chamber and the apical 5 mm of the root canals cultured. in addition to anaerobic incubation, duplicate cultures were incubated aerobically. fifty strains of bacteria from the 10 root canals were isolated and identified. the most prominent bacteria cultured from the 10 root canals were actinomyces, lactobacillus, black-pigmented bacteroides, peptostr ...19911809801
formation of nitrogen-containing metabolites from geniposide and gardenoside by human intestinal bacteria.during the course of our studies on the metabolism of iridoid glycosides by human intestinal bacteria, we found that geniposide (1) and gardenoside (4) were transformed to new nitrogen-containing compounds, genipinine (3) and gardenine (6), respectively, along with the known aglycones. although the amounts of new metabolites were somewhat lower than those of the aglycones, they were quantitatively analyzed by means of liquid chromatography/mass spectrometry (lc/ms). of 25 strains of human intest ...19911818345
defective neutrophil function in an insulin-dependent diabetes mellitus patients. a case report.the objective of this study was to evaluate the polymorphonuclear leukocyte (pmn) function in a poorly controlled adult insulin-dependent diabetic patient (iddm) with severe recurrent periodontitis, while describing the microbiological and clinical findings. chemotaxis, superoxide production, and phagocytosis and killing of porphyromonas (bacteroides) gingivalis by the iddm pmn were evaluated 1 week before treatment relative to a healthy, matched control. these analyses revealed a significant (p ...19911651389
percutaneous drainage of lung abscess in a malnourished child. 19911676504
[bacteriological examination of infections in the field of oral surgery].etiology of bacterial infections in the field of oral surgery was studied. a total of 270 samples collected from patients with encapsulated abscess in their oral cavities was examined and bacteria were isolated from the 244 samples (90.4%). the following results were found; 1) organisms more than one from one sample were frequently isolated from cases with parodontitis, pericoronitis and gnathitis. isolation of anaerobic bacteria was common (54.2%). 2) streptococcus milleri and streptococcus san ...19911791332
comparative in vitro activity of cefpodoxime against anaerobes other than bacteroides fragilis.to assess the in vitro activity of cefpodoxime against anaerobic respiratory tract and oropharyngeal pathogens 77 strains belonging to 18 gram-negative and 7 gram-positive species were studied by means of agar dilution tests. for comparison cefuroxime, amoxicillin, amoxicillin + clavulanic acid and clindamycin were also tested. cefpodoxime was found to be active at concentrations of less than or equal to 0.125 mg/l against prevotella oralis, prevotella buccalis, prevotella bivia, porphyromonas a ...19911800380
normal vaginal aerobic and anaerobic bacterial flora of the rhesus macaque (macaca mulatta).the most common bacterial species isolated from the vaginas of 37 healthy rhesus macaques were streptococcus viridans, coagulase negative staphylococcus, mobiluncus curtisii ss. curtisii, corynebacterium renale-like organisms, peptostreptococcus anaerobius, gardnerella-like organisms, and other corynebacterium species. the vaginal flora of the rhesus macaque differs from that previously reported for five other primate species. a two-year retrospective review of clinical cases of vaginitis and me ...19911803013
subgingival microflora of advanced periodontitis in the dominican republic.a study of the predominant subgingival microflora was carried out in 24 periodontitis patients, 18 to 60 years of age, in santo domingo, dominican republic. paper point sampling, transport in vmga iii, and conventional microbiological techniques were utilized. direct microscopic examination revealed that cocci and nonmotile organisms made up 85% of the total organisms and spirochetes as little as 3%. nonselective culturing showed gram-negative organisms to constitute 53% of total isolates. fusob ...19911658290
in-vitro activity of temafloxacin against anaerobic bacteria: a comparative study.the antimicrobial activity of temafloxacin for 328 anaerobic bacteria was determined and compared to that of cefotetan, cefoxitin, ciprofloxacin and metronidazole. the wadsworth agar dilution technique using brucella-lysed sheep blood agar was used throughout. at the recommended breakpoint concentration 4 mg/l, temafloxacin inhibited 60/62 (97%) of the isolates of bacteroides fragilis and 82/87 (94%) of the isolates of other species of the b. fragilis group. ninety-six percent of the 24 isolates ...19911664827
[anaerobes in infected canals: a preliminary study].anaerobes of 17 infected canals with periapical periodontitis were studied. most of root flora were not only anaerobes but also aerobes. anaerobes were predominant in chronic periapical periodontitis. major anaerobes isolated from canals were peptostreptococcus, b. melaninogenicus and b. oralis. the chi-square results indicated that the peptostreptococcus were significantly related to apical radiolucency and b. melaninogenicus were significantly related to percussion or foul smell. animal experi ...19911674465
[acute subdural empyema due to peptostreptococcus].a very rare case of acute subdural empyema due to peptostreptococcus was reported. a 11-year-old-girl was admitted to our hospital with high grade fever, unconsciousness and rt hemiparesis. ct scans showed the mass effect caused by the subdural empyema over the left frontotemporal region. subdural empyema was evacuated by the craniotomy. peptostreptococcus was found in the pus obtained during the operation. however, ct scans 10 days after the operation revealed another subdural empyema in the le ...19911681835
relation of baseline microbial parameters to future periodontal attachment loss.the relationship between the level of subgingival species at baseline and subsequent attachment loss in a subject was examined. 38 subjects (14-71 years) with prior evidence of periodontal destruction were monitored 2x for pocket depth and attachment level at 6 sites per tooth at baseline and 2 months. a subject was considered to exhibit new attachment loss if 1 or more sites increased 3 mm or more in attachment level in 2 months. subgingival plaque samples were taken at the baseline visit from ...19911661304
[2 cases of rhinogenic intracranial complications with normal rhinoscopy].two cases of non-postoperative rhinogenic intracranial complications are reported: patient 1 was a 25-year-old female with meningitis and right orbital phlegmon, and patient 2 was a 21-year-old male with a brain abscess. main symptoms in these patients were meningeal signs and high fever, and intranasal findings were normal in both patients. the diagnosis was not made until computed tomography revealed paranasal abnormalities. soon after surgical intervention and treatment with large doses of an ...19911896694
purification and characterization of nadp(+)-dependent 5,10-methylenetetrahydrofolate dehydrogenase from peptostreptococcus productus marburg.the 5,10-methylenetetrahydrofolate dehydrogenase of heterotrophically grown peptostreptococcus productus marburg was purified to apparent homogeneity. the purified enzyme catalyzed the reversible oxidation of methylenetetrahydrofolate with nadp+ as the electron acceptor at a specific activity of 627 u/mg of protein. the km values for methylenetetrahydrofolate and for nadp+ were 27 and 113 microm, respectively. the enzyme, which lacked 5,10-methenyltetrahydrofolate cyclohydrolase activity, was in ...19911899860
reactivity of igg from explant cultures of periapical lesions with implicated microorganisms.the presence of igg in periapical inflammatory lesions suggests that immune responses participate in the disease process. the purpose of this investigation was to study the reactivity of igg from the supernatant fluids of explant cultures of periapical lesions with microorganisms implicated in infections of endodontic origin. ninety periapical lesions that had been contiguous with the apex of a root were removed and maintained in explant cultures. a dot-enzyme-linked immunosorbent assay (dot-eli ...19911940739
specificity of antibodies present in human periapical lesions.various classes of immunoglobulins have been found in human periapical lesions. the specificity of secreted antibodies against antigens egressing from the root canal system has yet to be thoroughly investigated. the purpose of this study was to test the specificity of antibodies present in human periapical lesions. human periapical biopsies were removed and cultured as organ culture explants. antibodies present in the lesions were extracted in the cell culture fluids. a modified enzyme-linked im ...19911940740
microbiologic causes and neonatal outcomes associated with chorioamnion infection.chorioamnion infection is associated with histologic chorioamnionitis and prematurity, but the specific chorioamnion microorganisms associated with histologic chorioamnionitis, prematurity, and poor neonatal outcome have not been identified. bacteria were recovered from the chorioamnion cultures of 32% of 112 placentas delivered at less than or equal to 34 weeks' gestation and from 19% of 156 placentas delivered at greater than 34 weeks' gestation (odds ratio 2.1; 95% confidence interval 1.1 to ...19911951562
effects of propicillin on mixed continuous cultures of periodontal bacteria.experiments were designed to test the antibiotic (1-phenoxypropyl)penicillin (propicillin) against a complex microflora of periodontal bacteria. this was accomplished by using a continuously growing mixed culture that was obtained by enrichment of periodontal plaque in human serum. peptostreptococcus species, prevotella intermedia, lactobacillus, catenaforme, and streptococcus species were dominant members of the enrichment culture. none of the strains isolated from the enrichment culture exhibi ...19911952836
aerobic and anaerobic microbiology of acute suppurative parotitis.aspirates of pus from acute suppurative parotitis were studied for aerobic and anaerobic bacteria. bacterial growth was present in 23 specimens. a total of 36 bacterial isolates (20 anaerobic and 16 aerobic and facultative) were recovered, accounting for 1.6 isolates per specimen (0.9 anaerobic and 0.7 aerobic and facultative). anaerobic bacteria only were present in 10 (43%) patients, aerobic and facultatives in 10 (43%), and mixed aerobic and anaerobic flora in 3 (13%). single bacterial isolat ...19911992268
purification and properties of an iron-sulfur-containing and pyridoxal-phosphate-independent l-serine dehydratase from peptostreptococcus asaccharolyticus.l-serine dehydratase with a specific activity of 15 nkat/mg protein was present in the anaerobic eubacterium peptostreptococcus asaccharolyticus grown either on l-glutamate or l-serine. the enzyme was highly specific for l-serine with the lowest km = 0.8 mm ever reported for an l-serine dehydratase. l-threonine (km = 22 mm) was the only other substrate. v/km for l-serine was 500 times higher than that for l-threonine. l-cysteine was the best inhibitor (ki = 0.3 mm, competitive towards l-serine). ...19912065681
chronic prosthetic vascular graft infection visualized with technetium-99m-hexamethylpropyleneamine oxime-labeled leukocytes.technetium-99m-hmpao labeled leukocytes demonstrated chronic femoro-femoral prosthetic vascular graft infection several times during an 18-mo period in a 77-yr-old man. the intensity and distribution of the uptake in the graft were fluctuating in different imaging occasions possibly indicating the strength and location of the infection. gallium-67-citrate imaging showed negative results twice. the reason for negative 67ga results remained obscure. the infected graft was removed and the patient d ...19912066799
aerobic and anaerobic microbiology of peritonsillar abscess.thirty-four aspirates of pus from peritonsillar abscesses that were studied for aerobic and anaerobic bacteria showed bacterial growth. a total 107 bacterial isolates (58 anaerobic and 49 aerobic and facultative) were recovered, accounting for 3.1 isolates per specimen (1.7 anaerobic and 1.4 aerobic and facultatives). anaerobic bacteria only were present in 6 (18%) patients, aerobic and facultatives in 2 (6%), and mixed aerobic and anaerobic flora in 26 (76%). single bacterial isolates were reco ...19912000017
toothbrushing and transient bacteremia in patients undergoing orthodontic treatment.this study was made to determine whether and to what extent bacteremia occurred after toothbrushing in patients undergoing orthodontic treatment with fixed appliances. twenty patients were selected, all with negative history of heart or hematologic disorders. these patients had not taken antibiotics or had a history of a cold in the previous 30 days. blood samples of 20 ml were drawn before and 5 minutes after brushing. the immune status of the patients was tested by measurement of isohemaggluti ...19912028936
the laboratory identification of gram-positive anaerobic cocci.a collection of 256 clinical strains and 40 reference strains of gram-positive anaerobic cocci (gpac) was studied, to characterise the recognised species more fully and to define groups of strains which might correspond to previously undescribed species. the methods used were: gas-liquid chromatography (glc) for the detection of volatile fatty acids (vfas); determination of the pre-formed enzyme profile with a commercially available kit, atb 32a; microscopic appearance; colonial morphology; and ...19912030504
oral associated bacterial infection in horses: studies on the normal anaerobic flora from the pharyngeal tonsillar surface and its association with lower respiratory tract and paraoral infections.two hundred and seventy bacterial isolates were obtained from the pharyngeal tonsillar surface of 12 normal horses and 98 obligatory anaerobic bacteria were characterised. of these, 57 isolates belonging to 7 genera (peptostreptococcus (1); eubacterium (9); clostridium (6); veillonella (6); megasphera (1); bacteroides (28); fusobacterium (6)) were identified, and 16 of these were identified to species level (p. anaerobius (1); e. fossor (9); c. villosum (1); b. fragilis (1); b. tectum (2); b. he ...19912031304
pyoderma caused by peptostreptococcus tetradius in a pup.pyoderma caused by peptostreptococcus tetradius in a pup was successfully treated with cefadroxil. although this bacterium is rarely found in veterinary medicine, it is a known pathogen in human beings and should be considered as a potential causative agent in pyodermas in which anaerobes are suspected.19912061185
tetracycline resistance in peptostreptococcus species.of 15 peptostreptococcus sp. strains isolated between 1975 and 1984, 13 hybridized with the tet k, tet m, or tet o determinant. a donor peptostreptococcus anaerobius strain carrying the three determinants could transfer tet m to p. anaerobius and fusobacterium nucleatum recipients but not to an enterococcus faecalis recipient, while neither tet k nor tet o was transferred.19911929341
cerebral ventriculitis: mr findings.cerebral ventriculitis is an uncommon site and manifestation of infection of the central nervous system. we report the magnetic resonance findings in two patients with cytologic and csf culture proof of ventriculitis. the findings included abnormal signal intensity of the ependyma and csf on t2-weighted images. in addition, ependymal, meningeal, and dural enhancement was seen in one case, on t1-weighted images, after administration of gd-diethylenetriamine pentaacetic acid.19902179305
cephalexin concentrations in human serum, gingiva, and mandibular bone following a single oral administration.1. cephalexin concentrations in human serum, gingiva, and mandibular bone after a single oral administration of cephalexin (500 mg) were measured by the paper disc method. 2. the peak times of serum, gingiva, and mandibular bone were approximately 90, 120 and 120 min, respectively. 3. the peak concentrations of serum, gingiva, and mandibular bone were 10.58 micrograms/ml, 5.57 micrograms/g and 2.12 micrograms/g, respectively. 4. the concentration ratio of gingiva/serum and mandibular bone/serum ...19902276582
[mitral valve prolapse in infective endocarditis. incidence and characteristics].purpose valve prolapse (mvp) cases, complicated with infective endocarditis (ie), seen at a cardiologic reference hospital.19902260932
[effect of helium-neon-laser radiation on microbes].in a well-defined in-vitro experiment the effects of a he-ne-laser of 6 mw output on some dominating microbes in the oral cavity were studied by using a scanning electron microscope. no changes on the surface in the irradiated colonies could be seen even after an exposition lasting for 60 minutes (continuous or pulsated wave). therefore an antibacterial effect of softlaser-irradiation sometimes described in literature in questionable.19902270558
antibody response in immunized rabbits measured with bacterial immunoglobulin-binding proteins.protein g, an immunoglobulin (ig)-binding protein isolated from group c or g streptococci, binds to the fc portion of igg. protein l, from the anaerobic bacterium peptostreptococcus magnus, specifically binds light chains of ig. in this study, protein g and l were used to measure the production of antibodies in immunized rabbits. two rabbits were immunized with a mixture of human urinary proteins from a patient with tubular proteinuria, and blood samples were collected regularly from the animals ...19902273256
obligately anaerobic bacterial species isolated from foot-rot lesions in goats.lesions showing clinical signs of foot-rot from 120 goats were cultured on six selective media during october 1987 to november 1988. a total of 582 strictly anaerobic microorganisms belonging to 50 different species was isolated and identified. the anaerobes most frequently isolated belonged to the following genera: bacteroides (80%), peptostreptococcus (63.6%), megasphaera (40%), fusobacterium (29.2%), clostridium (22.5%), propionibacterium (12.5%), eubacterium (11.7%) and leptotrichia (10.8%). ...19902271912
the biotin-dependent sodium ion pump glutaconyl-coa decarboxylase from fusobacterium nucleatum (subsp. nucleatum). comparison with the glutaconyl-coa decarboxylases from gram-positive bacteria.membrane preparations of fusobacterium nucleatum grown on glutamate contain glutaconyl-coa decarboxylase at a high specific activity (13.8 nkat/mg protein). the enzyme was solubilized with 2% triton x-100 in 0.5 m nacl and purified 63-fold to a specific activity of 870 nkat/mg by affinity chromatography on monomeric avidin-sepharose. the activity of the decarboxylase was strictly dependent on na+ (km = 3 mm) and was stimulated up to 3-fold by phospholipids. the glutaconyl-coa decarboxylases from ...19902244788
[asporogenic anaerobic empyema--clinical and bacteriological investigations of 31 patients with anaerobic empyema].the author reviewed the records of 31 patients with asporogenic anaerobic empyema mostly seen in the wards of internal medicine, juntendo university hospital during the 27 years between 1961 and 1988, and obtained the following results. 1. there were 25 males and 8 females with an average age of 57.8 and 51.0 y/o (range, 25 to 79 y/o), respectively males more than forty years old occupied 74.2 percent of all cases. 28 patients (90%) had underlying conditions. 2. the cases of mixed infections wit ...19902258645
[anaerobic asporogenous infection in patients with postoperative empyema].the results of treatment of 38 patients with postoperative pleural empyema caused by anaerobic asporogenous infection were summarized. the factors contributing to the development of anaerobic asporogenous infection, peculiarities of the clinical picture, diagnosis and treatment were revealed, the measures for prophylaxis were outlined. the clinical effectiveness was achieved in 92.1% of the patients with lethality of 2.6%.19902280516
purification and comparative studies of dihydrolipoamide dehydrogenases from the anaerobic, glycine-utilizing bacteria peptostreptococcus glycinophilus, clostridium cylindrosporum, and clostridium sporogenes.three different dihydrolipoamide dehydrogenases were purified to homogenity from the anaerobic glycine-utilizing bacteria clostridium cylindrosporum, clostridium sporogenes, and peptostreptococcus glycinophilus, and their basic properties were determined. the enzyme isolated from p. glycinophilus showed the properties typical of dihydrolipoamide dehydrogenases: it was a dimer with a subunit molecular mass of 53,000 and contained 1 mol of flavin adenine dinucleotide and 2 redox-active sulfhydryl ...19902294086
late infection after total hip replacement, caused by an oral organism after dental manipulation. a case report. 19902295660
bacterial vaginosis as a risk factor for post-cesarean endometritis.bacterial species associated with bacterial vaginosis have been isolated more frequently from endometrial cultures of patients with postpartum endometritis than expected from the prevalence of bacterial vaginosis among pregnant women. to further assess the association between bacterial vaginosis and postpartum endometritis, vaginal gram smears were obtained from women admitted for delivery. vaginal smears of women delivered by cesarean were scored as normal or as indicating bacterial vaginosis. ...19902296423
splenic abscess due to peptostreptococcus spp. as a complication of cardiac catheterization.we describe the development of a splenic abscess, due in part to peptostreptococcus, as a complication of cardiac catheterization. the etiologies of splenic abscess should include peptostreptococcus, and splenic abscesses should be added to the list of complications of cardiac catheterization.19902317856
purification of nadph-dependent electron-transferring flavoproteins and n-terminal protein sequence data of dihydrolipoamide dehydrogenases from anaerobic, glycine-utilizing bacteria.three electron-transferring flavoproteins were purified to homogeneity from anaerobic, amino acid-utilizing bacteria (bacterium w6, clostridium sporogenes, and clostridium sticklandii), characterized, and compared with the dihydrolipoamide dehydrogenase of eubacterium acidaminophilum. all the proteins were found to be dimers consisting of two identical subunits with a subunit mr of about 35,000 and to contain about 1 mol of flavin adenine dinucleotide per subunit. spectra of the oxidized protein ...19902318809
relationship of bacteriologic characteristics to semen indices in men attending an infertility clinic.bacteria can be isolated from most seminal fluid samples, but the significance of these microorganisms is uncertain because most men lack symptoms associated with bacterial infection of the reproductive tract. we obtained semen samples from 37 men attending a special infertility clinic and assessed the relationship between seminal fluid microorganisms and seminal fluid analysis including sperm motility, morphology, and concentration; the numbers of polymorphonuclear leukocytes and other white bl ...19902325963
genetic basis of tetracycline resistance in urogenital bacteria.the distributions of the nucleotide sequences related to the tetracycline resistance determinants tet k, tet l, tet m, and tet o were studied by dot blot hybridization with randomly chosen clinical urogenital tract isolates of viridans group streptococci, streptococcus agalactiae, enterococcus faecalis, gardnerella vaginalis, lactobacillus spp., fusobacterium nucleatum, peptostreptococcus spp., and veillonella parvula. among the peptostreptococcus spp., 79% of the isolates hybridized with one (6 ...19902327774
biotransformations of aromatic aldehydes by acetogenic bacteria.vanillin was subject to o demethylation and supported growth of clostridium formicoaceticum and clostridium thermoaceticum. vanillin was also stimulatory to the co-dependent growth of peptostreptococcus productus. the aldehyde substituent of vanillin was metabolized by routes which were dependent upon both the acetogen and a co-metabolizable substrate (e.g. carbon monoxide [co]). c. formicoaceticum and c. thermoaceticum oxidized the aldehyde group of vanillin to the carboxyl level, while p. prod ...19902328911
polysaccharides from peptostreptococcus anaerobius and structure of the species-specific antigen.the cell-envelope antigens of peptostreptococcus anaerobius were extracted from intact cells by autoclave or alkaline treatment. the purified species-specific antigen (g) was identified among several polysaccharides obtained from the extracts by successive treatments with ribonuclease and pronase followed by ion-exchange and gel-filtration chromatography. g was investigated by 13c- and 31p-n.m.r. spectroscopy, titrimetry, elemental analysis, and gas-liquid chromatography. oxidation of g with nai ...19902076510
[susceptibilities of clinical bacterial isolates to antimicrobial agents. a study mainly focused on imipenem. reported by the research group for testing imipenem susceptibility on clinical isolates].this study was conducted to investigate susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antibacterial agents at 64 hospital laboratories throughout japan from september to december of 1988. in this study, identification and susceptibility testing were carried out at each laboratory and the tests were performed according to the disk dilution method recommended by nccls in which susceptibilities are classified into "s", "ms", "i" and "r". ipm showed markedly high in vit ...19902287060
the formation of hydrogen sulfide and methyl mercaptan by oral bacteria.the capacity to form volatile sulfur compounds was tested in bacteria isolated from subgingival microbiotas and in a representative number of reference strains. a majority of the 75 tested oral bacterial species and 7 unnamed bacterial taxa formed significant amounts of hydrogen sulfide from l-cysteine. the most active bacteria were found in the genera peptostreptococcus, eubacterium, selenomonas, centipeda, bacteroides and fusobacterium. methyl mercaptan from l-methionine was formed by some mem ...19902082242
bactericidal effects of human neutrophils and sera on selected endodontic pathogenic bacteria in an anaerobic environment.phagocytosis by normal human neutrophils and the bactericidal activity of normal human pooled serum were measured under anaerobic conditions on six endodontic pathogenic bacterial species from the genera actinomyces, bacteroides, fusobacterium, peptostreptococcus and streptococcus. the results revealed that actinomyces viscosus was not killed either by human neutrophils or by 20 per cent serum; peptostreptococcus anaerobius was not killed by either 20 per cent or 95 per cent serum; and bacteroid ...19902098335
perforated jejunal diverticulum with multiple hepatic abscesses.we have described a patient with multiple hepatic abscesses caused by a perforated jejunal diverticulum with a presumed route of infection via the portal vein. patients with hepatic abscesses and no known source of infection should be evaluated for a contained mesenteric perforation of the gastrointestinal tract. finally, in patients who fail to respond promptly to percutaneous catheter drainage of a liver abscess, a continuing source of infection, such as perforation of a jejunal diverticulum, ...19902105531
anaerobic bacteria and bacterial infections: perspectives on treatment and resistance in italy.results of laboratory tests of 2,000 samples obtained from 1984 to 1987 from patients with suspected anaerobic infections and the clinical experience of these patients are reported. of these samples, 395 were positive for anaerobes; 36.5% of these 395 samples contained single organisms, and 63.5% contained a mixture of anaerobes and aerobes. abdominal infections were the infections most frequently caused by anaerobes. the bacteroides fragilis group and strains of peptostreptococcus were the micr ...19902305181
[a clinical study of asymptomatic bacteriospermia].asymptomatic bacteriospermia was studied as an entity and its treatment was investigated in 65 subjects who clinically needed their semen tested. the obtained results are summarized as follows. 1. the rate of detection of bacteria in semen was 89.2%, and the rates of detection of aerobic and anaerobic bacteria were 55.4% and 70.8%, respectively. forty gram-positive strains were identified among the aerobic bacteria; rates of detection for staphylococcus epidermidis and enterococcus faecalis were ...19902112205
intra-abdominal abscess in children: a 13-year experience.the microbiology of intra-abdominal abscess in children is similar to that of adults--a mixture of aerobes and anaerobes. in abscess specimens taken from 36 children, predominant pathogens were bacteroides fragilis group species, peptostreptococcus species, and escherichia coli.19902120268
suppurative oral candidosis. review of the literature and report of a case.a case of a dento-alveolar abscess due to a polymicrobial infection with a yeast, candida guilliermondii and two other oral bacteria are described. a review of the literature has revealed only a single case of an odontogenic abscess due to candida species and we believe this to be the first report of a dentoalveolar infection where candida guilliermondii is associated as a co-pathogen.19902124592
a rapid diagnosis of anaerobic infection in the oro-maxillary region by gas-liquid chromatography.the relationship between volatile fatty acids (vfas) in pus and infecting bacterial species was examined in order to establish a rapid identification system for anaerobic microorganisms in purulent inflammation in the oro-maxillary region. vfas were detected by the direct injection of pus into gas-liquid chromatography (glc). bacterial examination was carried out by anaerobic culture using blood agar plates. the bacterial identification was carried out mainly according to the vpi manual. analysi ...19902131168
[significance of the bacterial flora in the etiology of apical periodontitis. qualitative, quantitative and topographical aspects].in the international literature it is shown the central role of root canal infection in the etiology of periapical lesions. as a matter of fact it has been proved (13) that sterile necrotic pulp tissue is completely unable to cause inflammatory reactions at the periapex. infection of endodontic origin extends to the supporting tissues of the tooth only in the case of their acute inflammation (e.g. acute apical periodontitis, acute alveolar abscess, phoenix abscess). on the other hand in chronic ...19902135040
[the immunological diagnosis of peptostreptococcal infection].the work deals with different methods for the diagnosis of anaerobic streptococcal infection, experimentally tested and clinically approved in the examination of children with acute pneumonia. the passive hemagglutination test, the immune rosette formation test and the specific lymphocyte blastogenesis test were used for diagnosis. as sensitin and antigen, the preparation of the peptostreptococcal bacterial mass was used. the diagnostic titer of antibodies to anaerobic streptococci was 1:160. a ...19902330780
clinical, pathologic, and microbiologic findings of foot abscess in neonatal pigs.a longitudinal study was undertaken in a swine herd with an ever-present problem of foot abscess in suckling pigs reared on a woven-wire floor. of 3,322 4-day-old pigs, 199 (6%) developed abscess lesions involving claws and accessory digits before weaning. lesions were first detected in 4-day-old pigs; median and mean ages at onset were 10 and 11.3 days, respectively. at first detection, most pigs had only a single claw affected, but 39 pigs had at least 2 claws with abscesses. hind limbs had mo ...19902351598
the obligate and facultatively anaerobic bacterial flora of the normal feline gingival margin.samples from the gingival margins of 14 cats considered normal on clinical examination were cultured for facultative and obligate anaerobic bacteria. all mouths were free from any gingival marginal inflammation and tartar build-up; all cats were between 6 and 12 months of age. a mixed growth was obtained from all samples. the mean number of bacterial species per sample was 10.7 with a range of 7-16 isolates. of the 150 isolates processed, 109 (72.66%) were obligate anaerobes. of the facultativel ...19902353448
characterization of the tet m determinants in urogenital and respiratory bacteria.tetracycline-resistant fusobacterium nucleatum, haemophilus ducreyi, mycoplasma hominis, peptostreptococcus spp., ureaplasma urealyticum, and veillonella parvula had dna sequences which showed homology throughout the length of the tet m transposon, tn916. in contrast, gardnerella vaginalis, commensal neisseria spp., and the 25.2-megadalton plasmid family lacked the complete transposon.19902159259
transferable tet m in fusobacterium nucleatum.two tetracycline-resistant fusobacterium nucleatum strains were able to transfer, by conjugation, tetracycline resistance and the tet m determinant to recipient f. nucleatum, peptostreptococcus anaerobius, and enterococcus faecalis strains at a frequency of 10(-1) to 10(-8).19902178339
optimum therapy for acute pelvic inflammatory disease.neisseria gonorrhoeae is responsible for about one-third to one-half of cases of acute pelvic inflammatory disease (pid), although there is considerable geographical variation. chlamydia trachomatis is also an important aetiological agent, and is currently isolated 4 times more commonly from the cervix than the gonococcus. however, it is now clear that acute pid is polymicrobial in aetiology. even when n. gonorrhoeae and/or c. trachomatis are isolated from the endocervix, anaerobes such as bacte ...19902190794
purification and properties of a nadh-dependent 5,10-methylenetetrahydrofolate reductase from peptostreptococcus productus.the methylenetetrahydrofolate reductase from the carbon-monoxide-utilizing homoacetogen peptostreptococcus productus (strain marburg) has been purified to apparent homogeneity. the purified enzyme catalyzed the oxidation of nadh with methylenetetrahydrofolate as the electron acceptor at a specific activity of 380 mumols.min-1 mg protein-1 (37 degrees c; ph 5.5). the apparent km for nadh was near 10 microm. the apparent molecular mass of the enzyme was determined by gel filtration to be approxima ...19902209595
the microbial flora from root canals and periodontal pockets of non-vital teeth associated with advanced periodontitis.microflora from root canals and periodontal pockets of periodontally affected teeth were compared in order to elucidate the as yet unknown relationship between pulpal and periodontal disease. caries-free teeth affected with advanced periodontitis and diagnosed as clinically dead by electric pulp testing were selected. the root canals and periodontal pockets were sampled, and the bacterial flora examined by both culture and interference microscopy. the results indicated that the aerobe/anaerobe r ...19902391182
peptostreptococcus hydrogenalis sp. nov. from human fecal and vaginal flora.we describe peptostreptococcus hydrogenalis sp. nov., which is found in human feces and vaginal discharge. this new species was established on the basis of the results of dna-dna hybridization among anaerobic cocci. the results of different biochemical reactions also are given. the type strain of this species is strain gifu 7662 (= jcm 7635).19902397199
[a case of cervico-mediastinal abscess, secondary to acute tonsillitis: investigation of the treatments].no other infectious diseases in the field of otolaryngology cause rapid and lethal course than cervical abscess. a case of cervicomediastinal abscess secondary to acute tonsillitis was presented. the patient was a 43-year-old male with liver cirrhosis and primarily had the treatment of tonsillitis. the complication of duodenal perforation caused marked general deteriotation, and cervical abscess occured. immediately after transfer to our department, he was treated by cervical drainage, laparotom ...19902213351
recolonization of the subgingival microflora after scaling and root planing in human periodontitis.the purpose of this study was to evaluate the recolonization patterns of the subgingival microflora of adult periodontitis patients after a single session of scaling and root planing. in each of eight patients, three clinically diseased sites were investigated microbiologically by darkfield microscopy and cultural analysis. after initial clinical and microbiological parameters were determined, each subject received a single session of scaling and root planing but no oral hygiene instructions. cl ...19902213468
formation of amphetamine from its nitro analogue by anaerobic intestinal bacteria.1. microbial reduction of 1-phenyl-2-nitropropane 1 was carried out using 40 strains of intestinal anaerobic bacteria. among them, 12 strains (mitsuokella multiacidus, clostridium perfringens, c. innocuum, c. clostiriiforme, c. difficile, c. butyricum, c. sp., eubacterium limosum, e. aerofaciens, e. multiforme, peptostreptococcus anaerobius and p. productus) had the ability to reduce 1 to amphetamine 2 (0.1-1% yield). 2. clostridium species were more active than another intestinal anaerobic bact ...19902219956
current taxonomy of medically important nonsporing anaerobes.this review deals mainly with the taxonomy of the genera and species in the family bacteroidaceae. it has been proposed that the genus bacteroides should be restricted to include the "bacteroides fragilis group" and that the asaccharolytic black-pigmented bacteroides species be transferred to a new genus, porphyromonas. new bacteroides, fusobacterium, and selenomonas species have been described. a high degree of heterogeneity is apparently present among peptostreptococcus species. mobiluncus is ...19902406865
use of modified norleucine-tyrosine broth in identification of peptostreptococcus anaerobius.gas-liquid chromatography was employed to analyze the volatile and nonvolatile acids produced in modified norleucine-tyrosine (mnt) broth by various gram-positive cocci. the mnt broth consists of 0.5% trypticase (bbl microbiology systems, cockeysville, md.), 0.5% yeast extract (difco laboratories, detroit, mich.), 0.2% l-norleucine, and 0.1% l-tyrosine. the microorganisms included reference strains and clinical isolates of peptostreptococcus spp. (p. anaerobius, p. asaccharolyticus, p. indolicus ...19902229394
aerobic and anaerobic bacteriology of wounds and cutaneous abscesses.the aerobic and anaerobic microbiologic characteristics of 584 wounds and 676 skin or soft-tissue abscesses were studied and correlated with the infection site. in wounds, aerobic or facultative bacteria only were present in 223 specimens (38%), anaerobes only in 177 specimens (30%), and mixed flora in 184 specimens (32%). in total there were 1470 isolates, 558 aerobic and 912 anaerobic, an average of 2.5 isolates per wound (1.6 anaerobic and 0.9 aerobic isolates). in abscesses, aerobic or facul ...19902241555
impending rupture of nonaneurysmal bacterial aortitis: ct diagnosis.the ct appearance of mycotic abdominal aortic aneurysms leading to eventual rupture has been well described. on the other hand, ruptured nonaneurysmal bacterial aortitis has not been demonstrated in the ct literature. we present two cases with subsequent rupture documented on ct. the characteristic findings of periaortic density and adjacent gas collection should suggest the diagnosis of acute aortic infection. this may herald impending rupture, even in the absence of aneurysmal dilatation, and ...19902229573
[a clinical study on ceftazidime in the treatment of intractable respiratory infections].ceftazidime (caz) was administered to 51 patients (37 males, 14 females) with respiratory infections including severe cases, accompanied by various underlying respiratory diseases. the clinical efficacy and side effects of caz were investigated. the mean age and body weight of these 51 cases were 62.6 years and 48.0 kg, respectively. caz was administered by intravenous drip infusion (daily dose of 2-4 g) for a mean of 14.7 days to a mean total dose of 56.7 g. clinical efficacy rates were 64% (18 ...19902232147
isolation and identification of anaerobic bacteria from ovine foot rot in spain.a microbiological study was made of 125 merino sheep showing clinical signs of foot rot. a total of 435 strictly anaerobic strains were isolated, belonging to the following genera: bacteroides, peptostreptococcus, tissierella, fusobacterium, megasphaera, eubacterium, acidaminococcus, clostridium, peptococcus and propionibacterium. of the 35 species obtained, the following were found in more than 10 per cent of animals sampled: bacteroides nodosus, b putredinis, b buccae, b ruminicola subspecies ...19902236925
septic shock complicating drainage of a bartholin gland abscess. 19902216255
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