Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| benthic ecology of vibrio spp. and pathogenic vibrio species in a coastal mediterranean environment (la spezia gulf, italy). | we carried out a 16-month in situ study to investigate the ecology of vibrio spp. and pathogenic vibrio species in coastal sediments of the mediterranean sea, employing multiple-regression analysis to reveal the major environmental factors controlling their occurrence in the benthic environment. in addition, association between vibrios and sediment-inhabiting meiofauna, which is a major component of benthic ecosystems, was investigated. culturable and total vibrio spp. estimates by most-probable ... | 2009 | 19543938 |
| infections in patients with aplastic anemia. | infection is a major cause of death in patients with aplastic anemia (aa). there are differences between the immunocompromised state of a patient with aa and the patient who is neutropenic due to chemotherapy and this leads to a difference in the infections that they incur. prolonged neutropenia is one of the largest risk factors for the development of infections with the invasive mycoses and bacteria. recovery from neutropenia is directly related to survival, and supportive care plays a large r ... | 2009 | 19549579 |
| comparative evolutionary analysis of the major structural subunit of vibrio vulnificus type iv pili. | type iv pili contribute to virulence in vibrio vulnificus, the bacterium responsible for the majority of fatal seafood-related infections. here, we performed within- and between-species evolutionary analysis of the gene that encodes the major structural subunit of the pilus, pila, by comparing it with pild and gyrb, the genes encoding the type iv prepilin peptidase and beta subunit of dna gyrase, respectively. although the diversity in pild and gyrb is similar to each other and likely to have ac ... | 2009 | 19556347 |
| roles of rseb, sigmae, and degp in virulence and phase variation of colony morphotype of vibrio vulnificus. | vibrio vulnificus is an estuarine bacterium capable of causing serious and often fatal wound infections and primary septicemia. we used alkaline phosphatase insertion mutagenesis to identify genes necessary for the virulence of this pathogen. one mutant had an in-frame fusion of 'phoa to the gene encoding rseb, a periplasmic negative regulator of the alternative sigma factor sigma(e). sigma(e) controls an extensive regulon involved in responding to cell envelope stresses. colonies of the rseb mu ... | 2009 | 19564391 |
| incidence of vibrio vulnificus in estuarine waters of the south texas coastal bend region. | to determine the occurrence of the human pathogen, vibrio vulnificus, in south texas coastal waters. | 2009 | 19566724 |
| iron differentially regulates gene expression and extracellular secretion of vibrio vulnificus cytolysin-hemolysin. | in a previous study, we showed that vibrio vulnificus is a ferrophilic bacterium that requires high levels of available iron for growth. in the present study, we show that iron stimulates, in an unusual manner, the production of cytolysin-hemolysin (vvha), the most potent exotoxin produced by v. vulnificus. the vvha gene possesses a putative ferric uptake regulator (fur)-binding box in the regulatory region, andvvha transcription was repressed by iron and de-repressed by fur mutation. however, e ... | 2009 | 19586415 |
| septic arthritis as the initial manifestation of fatal vibrio vulnificus septicemia in a patient with thalassemia and iron overload. | vibrio vulnificus infection is an uncommon but potentially fatal disease in children such that prompt recognition has prognostic implications. we describe here the case of a 9-year-old female with thalassemia and iron overload who presented with septic arthritis as an atypical initial manifestation of fatal v. vulnificus septicemia. this report underscores the possibility of septic arthritis as an early manifestation of v. vulnificus septicemia. pediatricians should be alert to this extremely in ... | 2009 | 19591224 |
| integrase-directed recovery of functional genes from genomic libraries. | large population sizes, rapid growth and 3.8 billion years of evolution firmly establish microorganisms as a major source of the planet's biological and genetic diversity. however, up to 99% of the microorganisms in a given environment cannot be cultured. culture-independent methods that directly access the genetic potential of an environmental sample can unveil new proteins with diverse functions, but the sequencing of random dna can generate enormous amounts of extraneous data. integrons are r ... | 2009 | 19596808 |
| the fragment structure of a putative hsdr subunit of a type i restriction enzyme from vibrio vulnificus yj016: implications for dna restriction and translocation activity. | among four types of bacterial restriction enzymes that cleave a foreign dna depending on its methylation status, type i enzymes composed of three subunits are interesting because of their unique dna cleavage and translocation mechanisms performed by the restriction subunit (hsdr). the elucidated n-terminal fragment structure of a putative hsdr subunit from vibrio vulnificus yj016 reveals three globular domains. the nucleolytic core within an n-terminal nuclease domain (ntd) is composed of one ba ... | 2009 | 19625490 |
| in vitro and in vivo antimicrobial activity of water-soluble chitosan oligosaccharides against vibrio vulnificus. | vibrio vulnificus is a gram-negative bacterium that induces severely rapid pathological progress. in this study, we evaluated the antibacterial activity of two water-soluble chitosan oligosaccharides, cos a (mw, 10,000 da) and cos b (mw, 1,000 da), from 90-95% deacetylated chitosan, against v. vulnificus in vitro and in vivo. treatment with cos a resulted in significantly higher suppressive effects on the growth of v. vulnificus than treatment with cos b. the growth of v. vulnificus was inhibite ... | 2009 | 19639224 |
| calcium is essential for the major pseudopilin in the type 2 secretion system. | the pseudopilus is a key feature of the type 2 secretion system (t2ss) and is made up of multiple pseudopilins that are similar in fold to the type 4 pilins. however, pilins have disulfide bridges, whereas the major pseudopilins of t2ss do not. a key question is therefore how the pseudopilins, and in particular, the most abundant major pseudopilin, gspg, obtain sufficient stability to perform their function. crystal structures of vibrio cholerae, vibrio vulnificus, and enterohemorrhagic escheric ... | 2009 | 19640838 |
| phase variation, capsular polysaccharide, pilus and flagella contribute to uptake of vibrio vulnificus by the eastern oyster (crassostrea virginica). | vibrio vulnificus infections are associated with raw oyster consumption, and disease reservoirs are determined by the ability of this bacterium to infect and persist in oysters. surface structures, such as capsular polysaccharide (cps), pili and flagella, function as virulence factors in mouse infection models. furthermore, virulence is related to phase variation in colony morphology, which reflects cps expression and includes opaque (encapsulated, virulent), translucent (reduced encapsulation, ... | 2009 | 19689704 |
| structure of a polysaccharide from the lipopolysaccharides of vibrio vulnificus strains cect 5198 and s3-i2-36, which is remarkably similar to the o-polysaccharide of pseudoalteromonas rubra atcc 29570. | high-molecular-mass polysaccharides were released by mild acid degradation of the lipopolysaccharides of two wild-type vibrio vulnificus strain, a flagellated motile strain cect 5198 and a non-flagellated non-motile strain s3-i2-36. studies by sugar analysis and partial acid hydrolysis along with (1)h and (13)c nmr spectroscopies showed that the polysaccharides from both strains have the same trisaccharide repeating unit of the following structure: -->4)-beta-d-glcpnac3nacylan-(1-->4)-alpha-l-ga ... | 2009 | 19695563 |
| rigorous vibrio vulnificus soft tissue infection of the lower leg in a renal transplant patient managed by vacuum therapy and autologous growth factors. | vibrio vulnificus is a gram-negative marine bacterium that grows well in coastal waters. it is an opportunistic pathogen that can cause serious life-threatening infections in patients with certain health conditions. vibrio-induced wound infections in immunosuppressed patients are difficult to treat because the healing process may be significantly delayed. reconstructive surgery may not be successful in early treatment as skin grafts are likely to fail, and there may be increased morbidity of don ... | 2009 | 19706229 |
| necrotizing soft-tissue infections and primary sepsis caused by vibrio vulnificus and vibrio cholerae non-o1. | vibrio species are a rare cause of necrotizing soft-tissue infections and primary septicemia, which are likely to occur in patients with hepatic disease, diabetes, adrenal insufficiency, and immunocompromised conditions. these organisms thrive in warm seawater and are often present in raw oysters, shellfish, and other seafood. this study examined fulminating clinical characteristics of vibrio vulnificus and vibrio cholerae non-o1 soft-tissue infections and identified outcome predictors. | 2009 | 19276771 |
| characterization of a new plasmid-like prophage in a pandemic vibrio parahaemolyticus o3:k6 strain. | vibrio parahaemolyticus is a common food-borne pathogen that is normally associated with seafood. in 1996, a pandemic o3:k6 strain abruptly appeared and caused the first pandemic of this pathogen to spread throughout many asian countries, america, europe, and africa. the role of temperate bacteriophages in the evolution of this pathogen is of great interest. in this work, a new temperate phage, vp882, from a pandemic o3:k6 strain of v. parahaemolyticus was purified and characterized after mitomy ... | 2009 | 19286788 |
| hlyu acts as an h-ns antirepressor in the regulation of the rtx toxin gene essential for the virulence of the human pathogen vibrio vulnificus cmcp6. | in vibrio vulnificus, hlyu upregulates the expression of the large rtx toxin gene. in this work we identified the binding site of hlyu to -417 to -376 bp of the rtxa1 operon transcription start site. lacz fusions for a series of progressive deletions from the rtxa1 operon promoter showed that transcriptional activity increased independently of hlyu when its binding site was absent. thus hlyu must regulate the rtxa1 operon expression by antagonizing a negative regulator. concomitantly we found th ... | 2009 | 19320834 |
| temperature effects on the depuration of vibrio parahaemolyticus and vibrio vulnificus from the american oyster (crassostrea virginica). | this study investigated temperature effects on depuration for reducing vibrio parahaemolyticus and vibrio vulnificus in american oyster (crassostrea virginica). raw oysters were inoculated with 5-strain cocktail of v. parahaemolyticus or v. vulnificus to levels of 10(4) to 10(5) mpn (most probable number)/g and depurated in artificial seawater (asw) at 22, 15, 10, and 5 degrees c. depuration of oysters at 22 degrees c had limited effects on reducing v. parahaemolyticus or v. vulnificus in the oy ... | 2009 | 19323759 |
| localization and characterization of vva0331, a 489-kda rtx-like protein, in vibrio vulnificus yj016. | vibrio vulnificus yj016 contains three genes encoding proteins homologous to repeats-in-toxin proteins. one of these genes, vva0331, possesses a long open reading frame of 13,971 bp in length and resides on the small chromosome between two gene clusters encoding a type i secretion system and several regulatory proteins, respectively. bioinformatic analysis revealed that vva0331 consist of nineteen 87-amino acid repeats, two arg-gly-asp motifs, four cysteine residues, an outer membrane protein do ... | 2009 | 19326097 |
| immune response and inhibition of bacterial growth by electrotransfer of plasmid dna containing the antimicrobial peptide, epinecidin-1, into zebrafish muscle. | bacterial infections represent serious diseases in aquaculture, rapidly leading to fish death by septicemia. we investigated whether the electrotransfer of green fluorescent protein gene fusion epinecidin-1 (cmv-gfp-epi) dna into zebrafish muscle could regulate the fish immune response and inhibit bacterial growth. electroporation parameters such as the number of pulses, voltage, and amount of plasmid dna were analyzed, and results demonstrated the greatest mrna expression level of gfp-epi relat ... | 2009 | 19340938 |
| [necrotizing fasciitis caused by a vibrio vulnificus infection in an eel fishery]. | 2009 | 19348100 | |
| hemochromatosis and vibrio vulnificus wound infections. | there are several reports of persons with hemochromatosis and vibrio vulnificus primary septicemia, but few accounts of persons with hemochromatosis and v. vulnificus wound infection. a 58-year-old white man developed infection of a forearm injury exposed to seawater in the gulf of mexico near the alabama coast. at age 66, he was diagnosed to have hemochromatosis. transferrin saturation was 89% and serum ferritin was 4761 pmol/l. hfe genotype was c282y/c282y. serum levels of hepatic enzymes, glu ... | 2009 | 19349902 |
| [vibrio species in the waters of southern sweden caused bath-wound fever. increased bacteria frequency according to studies on clams]. | 2009 | 19350770 | |
| structure of a polysaccharide from the lipopolysaccharide of vibrio vulnificus clinical isolate yj016 containing 2-acetimidoylamino-2-deoxy-l-galacturonic acid. | a polysaccharide isolated after mild acid degradation of the lipopolysaccharide of vibrio vulnificus clinical isolate yj016 was found to contain l-fuc, d-glcpnac, 2,4-diacetamido-2,4,6-trideoxy-d-glucose (di-n-acetylbacillosamine, d-quinac4nac), and 2-acetimidoylamino-2-deoxy-l-galacturonic acid (l-galnama). the last sugar derivative was confirmed by correlations for nitrogen-linked protons in 2d tocsy and roesy spectra measured in a h(2)o-d(2)o mixture. the following structure of the polysaccha ... | 2009 | 19368903 |
| growth and survival differences of vibrio vulnificus and vibrio parahaemolyticus strains during cold storage. | vibrio vulnificus and vibrio parahaemolyticus are the most common vibrio species associated with seafood illness in the united states. our study was conducted to determine if strain-to-strain differences exist in the growth and survival of 8 different v. vulnificus and v. parahaemolyticus strains at low temperatures. by day 10, v. vulnificus strain 515-4c2 had significantly higher counts (p < 0.05) (1.97 log cfu/g) compared with strains 3315, 1007, 29306 at 5 degrees c, which reached nondetectab ... | 2009 | 19723217 |
| occurrence of potentially pathogenic vibrios in final effluents of a wastewater treatment facility in a rural community of the eastern cape province of south africa. | we assessed the occurrence of vibrio pathogens in the final effluents of a rural wastewater treatment facility in the eastern cape province of south africa as free or plankton-associated (180 microm, 60 microm and 20 microm plankton sizes) entities using standard culture-based and molecular techniques. the free-living vibrio densities varied from 0 to 3.45 x 10(1) cfu ml(-1), while the plankton-associated vibrio densities vary with plankton sizes as follows: 180 microm (0-4.50 x 10(3) cfu ml(-1) ... | 2009 | 19732825 |
| discrimination of viable and dead vibrio vulnificus after refrigerated and frozen storage using ema, sodium deoxycholate and real-time pcr. | the effect of refrigerated and frozen storage on the viability of vibrio vulnificus was evaluated using cell suspensions (1x10(8) cfu/ml). ethidium bromide monoazide (ema) was utilized to selectively allow real-time (rti) pcr amplification of target dna from viable but not dead cells. bacterial survivors from the ema rti-pcr were evaluated by comparison with the plate count assay following different temperature exposures (-20 and 4 degrees c) every 24 h for 72 h. the log cfu values from the ema ... | 2009 | 19733199 |
| role of ntrc-regulated exopolysaccharides in the biofilm formation and pathogenic interaction of vibrio vulnificus. | vibrio vulnificus has been shown to require a global transcription factor, ntrc for mature biofilm development via controlling the biosyntheses of lipopolysaccharide and exopolysaccharide (eps). biofilm formation and eps production were dramatically increased in a medium including a tricarboxylic acid cycle-intermediate as a carbon source. these phenotypes required functional ntrc and were abolished by the addition of ammonium chloride. during the initial stage of biofilm formation, both express ... | 2009 | 19737353 |
| characterization of clinical and environmental types of vibrio vulnificus isolates from louisiana oysters. | vibrio vulnificus, a naturally occurring estuarine bacterium frequently transmitted to humans via raw oysters, is a leading cause of seafood-related deaths in the united states. although unique virulence markers have not been identified to date, multiple biomarkers have been used previously to associate strains with clinical or environmental types of v. vulnificus. in this study, we evaluated the usefulness of these biomarkers in characterizing 349 v. vulnificus oyster isolates by the presence/a ... | 2009 | 19743928 |
| epidemiologic study of vibrio vulnificus infections by using variable number tandem repeats. | a 3-year environmental and clinical vibrio vulnificus survey using simple-sequence repeats typing shows that v. vulnificus biotype 3 constitutes approximately 21% of the bacterium population in tested aquaculture ponds as opposed to approximately 86% of clinical cases. simple-sequence repeats proved to be a useful epidemiologic tool, providing information on the environmental source of the pathogen. | 2009 | 19751592 |
| ai-2 quorum-sensing inhibitors affect the starvation response and reduce virulence in several vibrio species, most likely by interfering with luxpq. | the increase of disease outbreaks caused by vibrio species in aquatic organisms as well as in humans, together with the emergence of antibiotic resistance in vibrio species, has led to a growing interest in alternative disease control measures. quorum sensing (qs) is a mechanism for regulating microbial gene expression in a cell density-dependent way. while there is good evidence for the involvement of auto-inducer 2 (ai-2)-based interspecies qs in the control of virulence in multiple vibrio spe ... | 2009 | 19778962 |
| effect of antimicrobial agents on the toll-like receptors and inflammatory cytokines in liver tissue of the alcohol-induced liver disease in rats with vibrio vulnificus sepsis. | septicemia and inflammation-mediated septic shock caused by vibrio vulnificus (vv) is strongly associated with chronic liver disease. this study examined the effects of antimicrobial therapy on expression of hepatic toll-like receptors and inflammatory cytokines in rats with alcohol-induced liver disease complicated by vv sepsis. | 2009 | 19781370 |
| multi-site analysis reveals widespread antibiotic resistance in the marine pathogen vibrio vulnificus. | vibrio vulnificus is a serious opportunistic human pathogen commonly found in subtropical coastal waters, and is the leading cause of seafood-borne mortality in the usa. this taxon does not sustain prolonged presence in clinical or agricultural settings, where it would undergo human-induced selection for antibiotic resistance. therefore, few studies have verified the effectiveness of commonly prescribed antibiotics in v. vulnificus treatment. here we screened 151 coastal isolates and 10 primary ... | 2009 | 18642041 |
| sensitive and rapid identification of vibrio vulnificus by loop-mediated isothermal amplification. | vibrio vulnificus is a serious bacterial pathogen for humans and aquatic animals. we developed a rapid, sensitive and specific identification method for v. vulnificus using loop-mediated isothermal amplification (lamp) technique. a set of primers, composed of two outer primers and two inner primers, was designed based on the cytolysin gene sequence of v. vulnificus. the lamp reaction was processed in a heat block at 65 degrees c for 60 min. the amplification products were detected by visual insp ... | 2009 | 18585020 |
| effect of in vitro exposure to vibrio vulnificus on hydroelectrolytic transport and structural changes of sea bream (sparus aurata l.) intestine. | the everted gut sac technique has been used to investigate the effect of vibrio vulnificus on water and electrolyte (na(+), k(+), cl(-), hco(3)(-)) transport on the intestine of sea bream (sparus aurata l.). both the anterior and the posterior intestine were incubated in a medium containing 10(8) v. vulnificus cells ml(-1) at 25 degrees c for 2 h. the presence of v. vulnificus resulted in a significant reduction (p < 0.05) of water absorption in the anterior intestine, while sodium absorption in ... | 2009 | 18825505 |
| enhancement of immunity and disease resistance in the white shrimp, litopenaeus vannamei, by the probiotic, bacillus subtilis e20. | effects of bacillus subtilis e20 isolated from fermented soybean on immune parameters and the disease resistance of the white shrimp (litopenaeus vannamei) after 98 days of b. subtilis e20 feeding were evaluated in this study. shrimp fed b. subtilis e20-containing diets at concentrations of 10(6) (e206), 10(7) (e207), and 10(8) (e208)cfu kg(-1), respectively, had significantly increased survival rates of 13.3%, 16.7%, and 20%, compared to the control (fed no probiotic) after being challenged wit ... | 2009 | 19111620 |
| nanobody-aided structure determination of the epsi:epsj pseudopilin heterodimer from vibrio vulnificus. | pseudopilins form the central pseudopilus of the sophisticated bacterial type 2 secretion systems. the crystallization of the epsi:epsj pseudopilin heterodimer from vibrio vulnificus was greatly accelerated by the use of nanobodies, which are the smallest antigen-binding fragments derived from heavy-chain only camelid antibodies. seven anti-epsi:epsj nanobodies were generated and co-crystallization of epsi:epsj nanobody complexes yielded several crystal forms very rapidly. in the structure solve ... | 2009 | 19118632 |
| structure of a polysaccharide from the lipopolysaccharide of vibrio vulnificus cect4602 containing 2-acetamido-2,3,6-trideoxy-3-[(s)- and (r)-3-hydroxybutanoylamino]-l-mannose. | a polysaccharide was isolated by gpc after mild acid treatment of the lipopolysaccharide of vibrio vulnificus cect4602 and found to contain l-rha, d-glcpnac and 2-acetamido-2,3,6-trideoxy-3-(3-hydroxybutanoylamino)-l-mannose (l-rhanac3nhb). glc analysis of the trifluoroacetylated (s)-2-octyl esters derived by full acid hydrolysis of the polysaccharide showed that approximately 80% of the 3-hydroxybutanoic acid has the s configuration and approximately 20% the r configuration. the following struc ... | 2009 | 19128797 |
| regulation of the vibrio vulnificus hupa gene by temperature alteration and cyclic amp receptor protein and evaluation of its role in virulence. | availability of free iron is extremely limited in the mammalian host, and the acquisition of iron in the host is essential for successful infection by pathogenic bacteria. expression of many genes involved in acquiring iron is regulated in response to the level of iron availability, and iron regulation is mediated by fur. in this study, cellular levels of vibrio vulnificus hupa, a heme receptor protein, and the hupa transcript were found to increase in cells grown at 40 degrees c compared to cel ... | 2009 | 19139193 |
| evaluation of genotypic and phenotypic methods to distinguish clinical from environmental vibrio vulnificus strains. | vibrio vulnificus is a heterogeneous bacterial species that comprises virulent and avirulent strains from environmental and clinical sources that have been grouped into three biotypes. to validate the typing methods proposed to distinguish clinical from environmental isolates, we performed phenotypic (api 20e, api 20ne, and biolog tests) and genetic (ribotyping and dna polymorphism at several loci) studies with a large strain collection representing different biotypes, origins, and host ranges. ... | 2009 | 19139234 |
| vibrio vulnificus cytolysin induces apoptosis in huvec, sgc-7901 and smmc-7721 cells via caspase-9/3-dependent pathway. | vibrio vulnificus cytolysin (vvc) is known to be a pore-forming toxin which shows cytotoxicity for mammalian cells in culture and induces apoptosis in endothelial cells. in order to determine whether vvc induces apoptosis in vascular endothelial cells and tumor cells, the cytotoxicity induced by recombinant vvc (rvvc) and its potential mechanism in huvec, sgc-7901 and smmc-7721 cells were investigated. our study demonstrated that rvvc induced the release of intracellular k(+) from all the target ... | 2009 | 19167479 |
| vibrio vulnificus-induced cell death of human mononuclear cells requires ros-dependent activation of p38 and erk 1/2 mapks. | vibrio vulnificus is a gram-negative bacterium that multiplies rapidly in host tissue and causes extensive tissue damage. human peripheral blood mononuclear cells (pbmc) were shown to be readily killed by exposure to live v. vulnificus. v. vulnificus induced production of intracellular reactive oxygen species (ros) and nitric oxide (no) in pbmc. pretreatment of pbmc with diphenyleneiodonium chloride (dpi) abolished ros generation upon exposure to v. vulnificus and decreased the bacterial ability ... | 2009 | 19172484 |
| complete sequence of plasmid pmp1 from the marine environmental vibrio vulnificus and location of its replication origin. | a novel cryptic plasmid, pmp1, from an environmental vibrio vulnificus mp-4 isolated from mai po nature reserve in hong kong, has been characterized. the 7.6-kb plasmid had guanine-cytosine content of 40.03% and encoded four open reading frames (orfs) with >100 amino acids. the predicted protein of orf1 contained 478 amino acids showing 29% identity and 50% similarity over 309 amino acids to the integrase of vibrio cholerae phage vp2. orf2 encoded a putative protein of 596 amino acids, which wer ... | 2009 | 19009320 |
| vibrio vulnificus: an unusual mode of acquisition and novel use of rapid susceptibility testing. | infection with vibrio vulnificus is uncommon in europe but is associated with necrotising wound infections and life-threatening septicaemia. this case is one of infection most likely to have been acquired from a thermal pool in turkey without preceding exposure to seawater or shellfish. the report also describes how early management was optimised using gradient diffusion antibiotic strips to provide rapid susceptibility data. | 2009 | 19028760 |
| expression of the cpda gene, encoding a 3',5'-cyclic amp (camp) phosphodiesterase, is positively regulated by the camp-camp receptor protein complex. | the intracellular level of cyclic 3',5'-amp (camp), a signaling molecule that mediates a variety of cellular processes, is finely modulated by the regulation of its synthesis, excretion, and degradation. in this study, camp phosphodiesterase (cpda), an enzyme that catalyzes the conversion of camp to amp, was characterized in a pathogenic bacterium, vibrio vulnificus. the cpda gene exists in an operon composed of mutt, yqib, cpda, and yqia, the transcription of which was initiated at position -22 ... | 2009 | 19028903 |
| structure of the lipopolysaccharide core of vibrio vulnificus type strain 27562. | the structure of the lipopolysaccharide core of vibrio vulnificus type strain 27562 is presented. lps hydrolysis gave two oligosaccharides, os-1 and os-2, as well as lipid a. nmr spectroscopic data corresponded to the presence of one kdo residue, one beta-glucopyranose, three heptoses, one glyceric acid, one acetate, three petn, and one 5,7-diacylamido-3,5,7,9-tetradeoxynonulosonic acid residue (pseudaminic acid, pse) in os1. os2 differed form os 1 by the absence of glyceric acid, acetate, and p ... | 2009 | 19185290 |
| proteomic analysis of vibrio vulnificus m2799 grown under iron-repleted and iron-depleted conditions. | vibrio vulnificus is an opportunistic marine bacterium that causes a serious, often fatal, infection in human. an important factor that determines the survival of v. vulnificus in the human body is the ability to acquire iron. the differential expression of proteins in whole-cell lysates of v. vulnificus m2799, a clinical isolate, was evaluated under iron-repleted and iron-depleted conditions during the early, mid and late logarithmic growth phases. a total of 32, 53 and 42 iron-regulated spots ... | 2009 | 19185608 |
| effects of escherichia coli rraa orthologs of vibrio vulnificus on the ribonucleolytic activity of rnase e in vivo. | rraa is a recently discovered protein inhibitor of rnase e that catalyzes the initial step in the decay and processing of numerous rnas in escherichia coli. in the genome of vibrio vulnificus, two open reading frames that potentially encode proteins homologous to e. coli, rraa-designated rraav1 and rraav2, have respectively 80.1% and 59.0% amino acid identity to rraa. the authors report that coexpression of rraav1 protein in e. coli cells overproducing rnase e rescued these cells from growth arr ... | 2009 | 19194749 |
| detection and enumeration of vibrio vulnificus by direct colony immunoblot. | a direct colony immunoblot method (dci) for the enumeration of vibrio vulnificus was developed. bacterial colonies were transferred from agar plates to membranes, which were then dried and blocked with bovine serum albumin. subsequently, the membranes were treated with anti-v. vulnificus h antibodies, washed and incubated with peroxidase-conjugated goat anti-rabbit igg. after a final wash, the membranes were exposed to a substrate mixture containing h(2)o(2) which resulted in the development of ... | 2009 | 19200106 |
| in vivo gene expression of cold shock and other stress-related genes in vibrio vulnificus during shellstock temperature control conditions in oysters. | to determine vibrio vulnificus response to shellstock refrigeration conditions while the bacterium was embedded in oysters (in vivo). | 2009 | 19200328 |
| plasmid diversity in vibrio vulnificus biotypes. | vibrio vulnificus is a heterogeneous bacterial species that can be virulent for humans and fish. virulence in fish seems to rely on a recently described plasmid that can be transmitted between strains, aided by a conjugative plasmid. the main objective of this work was to analyse the plasmid content of a wide collection of strains from the three biotypes of the species, as well as to identify putative conjugative and virulence plasmids by means of southern hybridization with specific probes and ... | 2009 | 19202097 |
| effects of flash freezing, followed by frozen storage, on reducing vibrio parahaemolyticus in pacific raw oysters (crassostrea gigas). | this study investigated the effects of flash freezing, followed by frozen storage, on reducing vibrio parahaemolyticus in pacific raw oysters. raw pacific oysters were inoculated with a five-strain cocktail of v. parahaemolyticus at a total level of approximately 3.5 x 10(5) most probable number (mpn) per gram. inoculated oysters were subjected to an ultralow flash-freezing process (-95.5 degrees c for 12 min) and stored at -10, -20, and -30 degrees c for 6 months. populations of v. parahaemolyt ... | 2009 | 19205481 |
| crystal structures of substrate and inhibitor complexes of ribose 5-phosphate isomerase a from vibrio vulnificus yj016. | ribose-5-phosphate isomerase a (rpia) plays an important role in interconverting between ribose-5-phosphate (r5p) and ribulose-5-phosphate in the pentose phosphate pathway and the calvin cycle. we have determined the crystal structures of the open form rpia from vibrio vulnificus yj106 (vvrpia) in complex with the r5p and the closed form with arabinose-5-phosphate (a5p) in parallel with the apo vvrpia at 2.0 a resolution. vvrpia is highly similar to eschericihia colirpia, and the vvrpia-r5p comp ... | 2009 | 19214439 |
| reduction of vibrio vulnificus in pure culture, half shell and whole shell oysters (crassostrea virginica) by x-ray. | the purpose of this investigation was to study the inactivation effect of x-ray treatments on inoculated vibrio vulnificus in pure culture, half shell and whole shell oysters to achieve a 5.0 log reduction, which is recommended by the interstate shellfish sanitation conference and the food and drug administration. a mixed culture of three v. vulnificus strains was used to prepare the pure culture and inoculated oysters. the pure culture and inoculated oysters were treated with 0.0, 0.1, 0.5, 0.7 ... | 2009 | 19217681 |
| spontaneous quinolone resistance in the zoonotic serovar of vibrio vulnificus. | this work demonstrates that vibrio vulnificus biotype 2, serovar e, an eel pathogen able to infect humans, can become resistant to quinolone by specific mutations in gyra (substitution of isoleucine for serine at position 83) and to some fluoroquinolones by additional mutations in parc (substitution of lysine for serine at position 85). thus, to avoid the selection of resistant strains that are potentially pathogenic for humans, antibiotics other than quinolones must be used to treat vibriosis o ... | 2009 | 19218407 |
| vibrio biofilms: so much the same yet so different. | vibrios are natural inhabitants of aquatic environments and form symbiotic or pathogenic relationships with eukaryotic hosts. recent studies reveal that the ability of vibrios to form biofilms (i.e. matrix-enclosed, surface-associated communities) depends upon specific structural genes (flagella, pili and exopolysaccharide biosynthesis) and regulatory processes (two-component regulators, quorum sensing and c-di-gmp signaling). here, we compare and contrast mechanisms and regulation of biofilm fo ... | 2009 | 19231189 |
| vibrio vulnificus: disease and pathogenesis. | 2009 | 19255188 | |
| a fatal case of vibrio vulnificus cellulitis with septicaemia. | vibrio vulnificus is a gram-negative marine bacterium that may cause local wound infection, distinctive soft tissue infection, gastroenteritis and septicaemia with a high mortality rate. a healthy man presented with severe abdominal pain, diarrhoea and fever followed by development of multiple blisters, cellulitis and necrotizing fasciitis of the lower limbs, who progressed rapidly to fulminant sepsis caused by this organism. vibrio vulnificus septicaemia should be suspected in the presence of s ... | 2009 | 20527281 |
| cadaverine is transported into vibrio vulnificus through its cadb in alkaline environment. | the exogenously-added cadaverine is effective in protecting vibrio vulnificus from methyl viologen (mv)-induced superoxide stress at ph 8.5. such a protective effect by cadaverine was not observed at ph 7.5. consistently, the accumulated level of intracellular cadaverine at ph 8.5 is approximately four times as much as that of the control cell at ph 7.5. cadaverine accumulation is not affected by mv. the protection of v. vulnificus by cadaverine from superoxide stress was abolished when cadb cod ... | 2009 | 19884768 |
| oligomerization is essential for apoptotic activity of vibrio vulnificus hemolysin. | vibrio vulnificus hemolysin (vvh), a pore forming toxin, is thought to be a virulence factor of this bacterium. it is well known that vvh induces apoptosis as well as cell lysis in susceptible target cells. although pore formation is an essential step in cell lysis, it is unknown whether this step is necessary for vvh-induced apoptosis. in this study, chinese hamster ovary (cho) cells were exposed to non-oligomerized mutant f334i, in which phenylalanine 334 was replaced by isoleucine. the rate o ... | 2009 | 19887751 |
| dietary medicinal plant extracts improve growth, immune activity and survival of tilapia oreochromis mossambicus. | the effects of supplementing diets with acetone extract (1% w/w) from four medicinal plants (bermuda grass cynodon dactylon, h(1), beal aegle marmelos, h(2), winter cherry withania somnifera, h(3) and ginger zingiber officinale, h(4)) on growth, the non-specific immune response and ability to resist pathogen infection in tilapia oreochromis mossambicus were assessed. in addition, the antimicrobial properties of the extract were assessed against vibrio alginolyticus, vibrioparahaemolyticus, vibri ... | 2009 | 20735646 |
| [tumoral hypocalcemia]. | hypocalcemia is a rare and not well understood paraneoplasic manifestation. we report the clinical case of a patient with hypocalcemia due to extensive osteoblastic metastasis. the corrected serum calcium levels for albumin was 7.2 mg/dl. calciuria was less than 10 mg/24 h. magnesium, phosphate and 25-hydroxyvitamin d levels were normal. pth and 1,25-dihydroxyvitamin d levels were increased. the pathogenesis of this disorder is possibly related to the massive calcium flow toward metastatic areas ... | 2009 | 2740546 |
| [decreasing the emission of pollutants in laboratory waste water by substitution of trichloromethane]. | working up great test series to determine chloroform-extractive substances and tensides, increased trichlormethane concentrations can appear in the laboratory air-inspite of realization of numerous precautionary measures. as a rule the makd rate will be exceeded than. various precautions failed to fulfill their purpose so that the solvent trichlormethane had been replaced by other solvents. two demands mainly influenced on this replacement. the risk to health must be decreased so far that the wo ... | 2009 | 2741501 |
| stress fracture of the rib in an elite oarsman. | this paper documents an unusual case of a stress fracture of the ninth rib in an elite oarsman. a 25-year-old male presented with a 3-day history of right-sided chest pain aggravated by deep inspiration and movements simulating rowing. the athlete was treated initially as having a soft tissue injury; however, a technetium-99 bone scan confirmed the diagnosis. routine radiographs of the ribs were negative. the pain and symptoms were confined to the anatomical area of the fracture along the antero ... | 2009 | 2777439 |
| reversal of rejection-induced coronary vasculitis detected early after heart transplantation with increased immunosuppression. | four patients who underwent heart transplantation, in whom coronary obstruction was seen early after transplantation, are described. repeated acute rejection episodes were detected within the first 2 months in each patient. coronary obstruction or ischemia was shown through a combination of t1-201 isotopic study findings, evidence of vasculitis of a small coronary arteriole seen at endomyocardial biopsy, or coronary angiographic results. vigorous treatment for rejection (antithymocyte globulin a ... | 2009 | 2795284 |
| the effect of six months continuous subcutaneous insulin infusion on kidney function and size in insulin-dependent diabetics. | glomerular filtration rate (gfr), renal plasma flow (rpf), kidney volume, and urinary albumin excretion rate were measured in 24 insulin-dependent diabetics, aged 29 +/- 7 years (mean +/- s.d.) of 8 +/- 4 years duration, randomly allocated to either continuous subcutaneous insulin infusion (csii) (n = 12) or unchanged conventional insulin treatment (cit) (n = 12). glomerular filtration rate, renal plasma flow, and kidney volume were identical in the two groups at the start of the study, although ... | 2009 | 2951131 |
| safety of intraaortic balloon counterpulsation in patients with acute myocardial infarction receiving streptokinase intravenously. | 2009 | 2801563 | |
| [the results of an investigation of the various factors influencing the late patency ratio of coronary bypass grafting]. | the purpose of this communication was to find out the factors influencing the late patency ratio of coronary artery bypass grafts of 42 patients in our institution. the following results were obtained: 1. the size of the measured diameters of coronary arteries and the graft flow rates were correlated well to the late patency ratio (p less than 0.05). 2. the regular physical exercise trainings were significantly important to keep patent grafts (p less than 0.01). 3. in blood serum studies, serum ... | 2009 | 2786538 |
| a photographic study of enamel defects among south australian school children. | colour photographs were taken of the anterior teeth of 1757 consenting 8-9 year old primary school children in fluoridated and non-fluoridated areas of south australia. children with a lifelong exposure to fluoridated water were significantly more likely to have blemished permanent incisor teeth than the children who had never resided in a fluoridated area. children who resided in a non-fluoridated area but had taken fluoride supplements at some stage in their first five years had a prevalence o ... | 2009 | 2818303 |
| pilomatrix carcinoma: role played by mr imaging. | we report the magnetic resonance imaging (mri) of a pilomatrix carcinoma. we found a soft tissue tumor of the back entering the spinal canal and compressing the spinal cord and we monitored a good response to radiotherapy and chemotherapy. we have concluded that mri played an important role in determination of the volume, extension and management of this rare malignant tumor. | 2009 | 2747902 |
| changing the school system. | 2009 | 2738019 | |
| proliferating giant pigmented nevus: a report of an unusual tumor occurring in association with a congenital giant pigmented nevus. | a 32-year-old japanese woman with a giant pigmented congenital nevus of the torso presented with a massive pigmented tumor mass of the vulva which grew over an 8-year period. histologically, the tumor was composed of benign appearing nevus-like cells with focal areas of extensive fibrous response. the tumor cells were positive for s-100 protein and with an antihuman melanoma antibody (moab 225, 28s) stain. electron microscopy confirmed the nevomelanocytic nature of the tumor cells and demonstrat ... | 2009 | 2715665 |
| [conventional insulin therapy, infusion pumps, injector syringes. different methods of administration]. | 2009 | 2665113 | |
| [occupational diseases and their reporting]. | 2009 | 2734931 | |
| stereotactic evacuation of hypertensive cerebellar hemorrhage using plasminogen activator. | treatment for hypertensive cerebellar hemorrhage still remains controversial as to whether direct surgical procedure is indicated or not. this is so even after the introduction of ct scan which easily demonstrates the location and size of the hematoma and the presence of hydrocephalus. in this paper, we present our experience of 20 patients with cerebellar hemorrhage treated by stereotactic evacuation using komai's ct-stereotactic apparatus. all the patients had vertigo, cerebellar symptoms, dys ... | 2009 | 2674756 |
| [a different view of sykstus lewkowicz]. | 2009 | 2698489 | |
| [the place of provisional tissues in the histologic classification, used for pedagogical purposes]. | 2009 | 2730350 | |
| new system helps prevent medi-cal claim delays. | 2009 | 2649431 | |
| [the link between cholesterol and atherosclerosis: intervention trials]. | intervention trials aimed at influencing the course of atherosclerosis by reducing blood cholesterol levels of two types: some of them evaluate the clinical consequences of atherosclerosis, while the others study the disease itself by means of arteriography. several trials of the first type have convincingly shown that the incidence of coronary disease decreases after at least 5 years of medicinal treatment. as for direct visualization trials, they seem to indicate that the development of athero ... | 2009 | 2662362 |
| no association between post-transplant erythrocytosis, thromboembolic events, and cyclosporine therapy. | 2009 | 2652691 | |
| the conversion of [35s] ptc-angiotensin i to ptc-angiotensin ii in plasma of normotensive and hypertensive subjects. | 2009 | 4313539 | |
| [poisoning by geromitra esculenta]. | gyromitra esculenta (persoon ex fries) mushrooms have been responsible for severe intoxications and even deaths. clinical data are characterized primarily by vomiting and diarrhoea, and, afterwhile, by jaundice, convulsions and coma. the species of concern are mainly g. esculenta, g. fastigiata and g. gigas; nevertheless, recent advances in chromatography, biochemistry and toxicology have established that other species within the ascomycetes may prove also toxic. the toxins, i.e. gyromitrin (n-m ... | 2009 | 2681713 |
| weakness in patients with hemiparesis. | clinical and experimental results are reviewed concerning muscle weakness in patients with hemiparesis after a stroke. the discussion includes the important role that alterations in the physiology of motor units, notably changes in firing rates and muscle fiber atrophy, play in the manifestation of muscle weakness. this role is compared with the lesser role that spasticity (defined as hyperactive stretch reflexes) of the antagonist muscle group appears to play in determining the weakness of agon ... | 2009 | 2655457 |
| cannabis--its clinical effects. | the range of clinical, intoxicating, psychological and psychiatric effects of cannabis ('dagga') are reviewed. controversial subjects, such as the entities of toxic cannabis psychosis and the cannabis amotivational syndrome, are discussed. | 2009 | 2669170 |
| evaluation of infant feeding in acute gastroenteritis. | sixty-eight bottle-fed babies under 9 months of age with mild acute gastroenteritis were observed to evaluate current feeding regimens following acute gastroenteritis in infancy. all babies were fed for 24 h with a glucose-electrolyte mixture (gem) and then randomly assigned to either a gradual reintroduction to their normal milk, i.e., slow regrade; immediate return to full-strength formula; or a rapid regrade to a hypoallergenic whey hydrolysate formula. all groups were well matched for age, s ... | 2009 | 2709254 |
| the incidence and significance of seminal vesicle invasion in patients with adenocarcinoma of the prostate. | the incidence and significance of seminal vesicle invasion in patients with adenocarcinoma of the prostate were determined in 139 patients who underwent radical prostatectomy. of the 36 patients who had seminal vesicle invasion, 27 (75%) also had capsular invasion and 13 (43%) had lymph node metastases. disease progression rates were 31% and 58% at 5 and 8 years, respectively. survival rate at 5 years was 90.6% and at 8 years it was 83.7%. of the 103 patients without seminal vesicle invasion, 21 ... | 2009 | 3815316 |
| comparative dna reassociation kinetics of cranes. | 2009 | 2775167 | |
| comparative dna reassociation kinetics of cranes. | 2009 | 2775167 | |
| effects of public day-care: a longitudinal study. | 119 swedish children were followed from their first year of life up to the age of 8. most could be classified according to (a) type of day-care they had experienced during their first 7 years of life and (b) time of first entrance into day-care. at 8 years, the children were tested with aptitude tests and rated by their teachers on school performance and social and personal development. hierarchical regression analyses and mancovas were used in the statistical treatment. time of entrance into da ... | 2009 | 2758882 |
| [ultrasonic diagnosis of tumorous lesions of the liver]. | 2009 | 2698513 | |
| ultrastructural analysis of axosomatic contacts on functionally identified primate spinothalamic tract neurons. | the morphology and frequency of axosomatic contacts on three functionally identified primate spinothalamic tract (stt) cells were analyzed at the electron microscopic level. the stt cells analyzed were wide-dynamic-range neurons responsive to activation of low- and high-threshold cutaneous afferents innervating the foot. the somas were located in the lateral border of lamina v; the dendritic trees were oriented dorsally and were very extensive. numerous spinelike appendages were observed emanati ... | 2009 | 2708581 |
| hypersensitivity myocarditis and hepatitis associated with imipramine and its metabolite, desipramine. | we present two cases of myocarditis and hepatitis with histologic characteristics of hypersensitivity-mediated drug reactions associated with imipramine and its metabolite, desipramine. in one case, death was directly attributed to myocarditis; in the second case, the patient died of an acute myocardial infarct, but myocarditis may have played a contributory role. one patient was taking imipramine, and therapeutic concentrations of imipramine and desipramine were documented in postmortem blood. ... | 2009 | 2760582 |
| [effect of glucocorticosteroid therapy on immunologic indicators in patients with sarcoidosis and idiopathic fibrosing alveolitis]. | immunological findings were recorded in 22 patients with active sarcoidosis and 12 patients with idiopathic fibrosing alveolitis. in addition, the patients' endopulmonary cytograms were considered. immunological evidence involved the use of bronchoalveolar lavage, bronchoscopy, peripheral blood. administration of corticosteroid therapy resulted in alveolitis attenuation both in sarcoidosis and fibrosing alveolitis. common features of immune response of sarcoidosis and idiopathic fibrosing alveol ... | 2009 | 2811240 |
| renal lesions following viper bites--a study of 14 years. | 2009 | 3759161 | |
| is there still a place for conjunctival-approach ptosis surgery? | 2009 | 2687185 | |
| [necrosis of the bone marrow]. | the authors analyzed the incidence of necrosis of the bone marrow in nonselected autopsy material. the studies included 419 cases of various disease types. necrosis of the bone marrow was found in 83 cases (19.8%). it was most frequently observed in patients with neoplastic disease of the hematopoietic and lymphatic systems (26.3%) and malignant neoplasms of different origin (21.9%). in 40% cases necrosis of the bone marrow were accompanied by neoplastic metastases into the hematopoietic tissue, ... | 2009 | 2637982 |
| [periapical lesions treated with cao. histological controls]. | the results of histological examinations conducted on 2 granulomas found in teeth and treated with calcium oxide are reported. it is concluded that the treatment produces significant changes in the histological picture with the formation of scar tissue that precedes the growth of newborn in a relatively short time. | 2009 | 2770661 |
| carotid cave aneurysms. | 2009 | 2635881 | |
| how fast do very small abdominal aortic aneurysms grow? | fifty patients with abdominal aortic aneurysms from 2.5 to 5.0 cm in anteroposterior diameter (median 3.1 cm, mean +/- s.e. 3.3 +/- 0.1 cm) were initially offered non-operative treatment. two patients have subsequently undergone successful elective aneurysm resection because of increase in aneurysm size, and a third has died. the median annual growth rate of the aneurysm has been 0.22 cm and 77.8% increased in size between 6 monthly ultrasound examination. for aneurysms less than 4.0 cm the maxi ... | 2009 | 2714450 |
| effect of a high-fat meal on the bioavailability of a polymer-coated erythromycin particle tablet formulation. | the effect of food on the relative bioavailability of an erythromycin particles-in-tablet formulation was studied in 27 healthy volunteers, using a four-way, crossover study design with the following treatments: one or two erythromycin capsules usp (eryc, parke-davis), or one polymer-coated erythromycin particles-in-tablet (pce, abbott) administered fasting or with a high-fat meal. under fasting conditions the erythromycin particles-in-tablet and erythromycin capsule formulations are bioequivale ... | 2009 | 2708552 |