Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| polarizing response of human polymorphonuclear cells to vibrio cholerae. | leucocytes have the capacity to respond to chemotactic factors by becoming morphologically and functionally polarized and this method has been found to be suitable for measurement of chemotaxis. this work evaluates the effect of whole vibrio cholerae biotype el tor polarization of human polymorphonuclear (pmn) cells in comparison with strains of v. cholerae nag, vibrio alginolyticus and salmonella typhi. v. cholerae o1 induced, at different cell/bacteria ratios, a significant increase in the per ... | 1997 | 9503599 |
| genetic defect of the sodium pump-defective mutant nap-1 from the marine vibrio alginolyticus. | the marine bacterium, vibrio alginolyticus, has a respiratory chain-linked na(+)-translocating nadh-quinone reductase (nqr). among several mutant cells defective in na+ pump activity, nap1 was a very stable mutant and a spontaneous revertant could not be isolated from nap1. using genetic information from the recently sequenced nqr operon, the genetic defects in nap1 were examined, and the sodium pump-defective mutant nap1 was found to be caused by the insertion of a 1.2 kbp dna fragment into the ... | 1997 | 9043633 |
| alkaline serine protease is an exotoxin of vibrio alginolyticus in kuruma prawn, penaeus japonicus. | an extracellular lethal toxin produced by vibrio alginolyticus strain swy originally isolated from diseased kuruma prawn(penaeus japonicus) was partially purified by fast protein liquid chromatography with hydrophobic interaction (phenyl sepharose hig hperformance) chromatography and gel filtration columns. the toxin is an alkaline serine protease, inhibited by phenyl-methylsulfonyl fluoride (pmsf),and showed maximal activity at ph 10, having a molecular weight of about 33kda estimated by sds-pa ... | 1997 | 9003588 |
| [provisional crowns and bridges]. | 1997 | 3250891 | |
| [free radicals in the flavin reduction reaction. interaction of reduced nicotinamide adenine dinucleotide with flavin in solution]. | the study of the flavin mononucleotide (fmn)-reduced nicotinamide adeninedinucleotide (nadh) reaction was carried out both under aerobic and anaerobic conditions, using spectrophotometric and titrimetric methods. the consumption of nadh was shown to exceed two times the consumption of fmn in the anaerobic reaction and the rate constant in the aerobic reaction was found to be about 4 times of that of the anaerobic reaction. moreover, the replacement of anaerobic conditions by aerobic ones at ph 5 ... | 1997 | 179618 |
| new frontier for radiology: computed tomography. 40th annual preston m. hickey memorial lecture. | the basic principles of computed tomography (ct) and their application are discussed. the impact of this method on neuroradiologic diagnosis is reviewed, including economic considerations and the effect on nuclear medicine and other diagnostic procedures. the initial experiences of the mallinckrodt institute with whole body scanning are illustrated, and failure possibilities for ct are predicted. | 1997 | 179368 |
| teaching discharge planning. | 1996 | 177953 | |
| mixed tumors of the salivary glands. growth pattern and recurrence. | in 112 patients with mixed tumors of the salivary glands, 94 tumors were located in parotid glands, 11 in submandibular salivary glands, and 7 in monor palatal salivary glands. capsular ingrowth, a characteristic growth pattern, may explain the frequency of multilobulation and capsular defects in these tumors, particularly those of long standing. the capsular thickness, the extent of fibrosis with stromal hyalinization, and the degree of lymphocytic infiltration within and around the capsule, we ... | 1996 | 178290 |
| nadh:ubiquinone oxidoreductase of vibrio alginolyticus: purification, properties, and reconstitution of the na+ pump. | the na+-activated nadh:ubiquinone oxidoreductase of vibrio alginolyticus was extracted from the membranes with lauryldimethylamine-n-oxide and purified by two successive anion exchange columns. this preparation, yielding four major and several minor stained bands after sds-page, retained the nadh-dehydrogenase activity (with menadione as an artificial electron acceptor) and ubiquinone-1 (q) reductase activity. on further fractionation of the enzyme, the q-reductase activity essentially disappear ... | 1996 | 8639563 |
| existence of na+-translocating nadh-quinone reductase in haemophilus influenzae. | we previously cloned and sequenced nqr operon encoding the na+-translocating nadh-quinone reductase (nqr) from the marine bacterium vibrio alginolyticus. a gene cluster very similar to nqr operon was found to exist in the genome of haemophilus influenzae rd. we examined the membrane fraction from h. influenzae, and the respiratory chain of h. influenzae was found to contain a na+-dependent nqr that is essentially identical to those found in the marine v. alginolyticus. these results indicate tha ... | 1996 | 8601449 |
| cloning and characterization of moty, a gene coding for a component of the sodium-driven flagellar motor in vibrio alginolyticus. | the bacterial flagellar motor is a molecular machine that couples proton or sodium influx to force generation for driving rotation of the helical flagellar filament. in this study, we cloned a gene (moty) encoding a component of the sodium-driven polar flagellar motor in vibrio alginolyticus. nucleotide sequence analysis revealed that the gene encodes a 293-amino-acid polypeptide with a single putative transmembrane segment that is very similar (94.5% identity) to the recently described moty of ... | 1996 | 8636046 |
| expression and analysis of the gene for the catalytic beta subunit of the sodium-translocating nadh-ubiquinone oxidoreductase of vibrio alginolyticus. | 1996 | 8674603 | |
| rotational fluctuation of the sodium-driven flagellar motor of vibrio alginolyticus induced by binding of inhibitors. | rotation of the na(+)-driven flagellar motor of vibrio alginolyticus was investigated under the influence of inhibitors specific to the motor, amiloride and phenamil. the rotation rate of a single flagellum on a cell stuck to a glass slide was examined using laser dark-field microscopy. in the presence of 50 mm nacl, the average rotation rate (omega) was about 600 r.p.s. with a standard deviation (sigma omega) of 9% of omega. when omega was decreased to about 200 r.p.s. by the presence of 1.5 mm ... | 1996 | 8683575 |
| cloning and sequencing of nhab gene encoding an na+/h+ antiporter from vibrio alginolyticus. | a gene has been cloned from the marine bacterium vibrio alginolyticus that functionally complements a mutant strain of escherichia coli, to114, defective in three na+/h+ antiport genes (nhaa, nhab, chaa). the nucleotide sequence of the cloned fragment revealed an open reading frame, which encodes a protein with a predicted 528 amino acid sequence and molecular mass of 57212 da. this gene has 62% identity to nhab gene at the dna level from escherichia coli and the deduced amino acid sequence is 6 ... | 1996 | 8695633 |
| the bacillus stearothermophilus nub36 sura gene encodes a thermophilic sucrase related to bacillus subtilis saca. | the complete nucleotide sequence of the sura gene, encoding a sucrase from bacillus stearothermophilus nub36, was determined. sura was composed of 1338 bp and encoded 445 amino acid residues. the deduced polypeptide of m(r) 51519 showed strong sequence similarity to sucrose and sucrose phosphate hydrolases from bacillus subtilis, klebsiella pneumoniae and vibrio alginolyticus, and contained the 'sucrose box' residues thought to be important for catalysis of the transfer of fructose from sucrose. ... | 1996 | 8757729 |
| effect of viscosity on swimming by the lateral and polar flagella of vibrio alginolyticus. | by using mutants of vibrio alginolyticus with only a polar flagellum (pof+ laf-) or only lateral flagella (pof- laf+), we examined the relationship between swimming speed and the viscosity of the medium for each flagellar system. pof+ laf- cells could not swim in the high-viscosity environment (ca. 200 cp) in which pof- laf+ cells swam at 20 microns/s. the pof- laf+ cells swam at about 20 microns/s at normal viscosity (1 cp) without the viscous agent, and the speed increased to 40 microns/s at a ... | 1996 | 8759871 |
| attachment of vibrio alginolyticus to chitin mediated by chitin-binding proteins. | vibrio alginolyticus is the only culturable vibrio associated with the chitinaceous carapace of the copepod tigriopus fulvus (fisher 1860) living in ligurian coastal rock pools (tyrrhenian sea). the characteristics of the interaction between chitin particles and v. alginolyticus were studied by analysing strains isolated both from the copepod surface and from rock-pool water. the highest degree of attachment to chitin was observed at 20 degrees c, in the presence of 3% nacl. bacterial treatment ... | 1996 | 8760933 |
| comparison of a fluorogenic assay with a conventional method for rapid detection of vibrio parahaemolyticus in seafoods. | a conventional method and a fluorogenic assay for the detection of vibrio parahaemolyticus were compared. among 29 seafood samples examined for the presence of v. parahaemolyticus, 17 samples harbored v. parahaemolyticus, and trypsinlike activity was noticed in 19 seafoods. the added fluorogenic substrate was cleaved in single samples of shrimp, turbo, and cuttlefish from which v. parahaemolyticus could not be isolated by the conventional method. vibrio alginolyticus, in addition to v. parahaemo ... | 1996 | 8795246 |
| chemotactic responses to an attractant and a repellent by the polar and lateral flagellar systems of vibrio alginolyticus. | chemotactic responses in vibrio alginolyticus, which has lateral and polar flagellar systems in one cell, were investigated. a lateral-flagella-defective (pof+ laf-) mutant, which has only a polar flagellum, usually swam forward by the pushing action of its flagellum and occasionally changed direction by backward swimming. when the repellent phenol was added, pof+ laf- cells moved frequently forward and backward (tumbling state). the tumbling was derived from the frequent changing between counte ... | 1996 | 8885393 |
| virulence of vibrio alginolyticus isolated from diseased tiger prawn, penaeus monodon. | outbreaks of mass mortality among cultured tiger prawns (penaeus monodon) with white spotted syndrome (wss) in the carapace occurred in the summer of 1994 in i-lan, taiwan. a swarming strain val was isolated from hemolymph of the moribund prawns with tryptic soy agar (tsa, supplemented with 1% nacl, oxoid) and/or thiosulfate citrate bile salt sucrose (tcbs, difco) agar. this strain was characterized and identified to be vibrio alginolyticus. the strain was susceptible to antibiotics such as chlo ... | 1996 | 8867464 |
| isolation and characterization of vibrio alginolyticus isolated from diseased kuruma prawn, penaeus japonicus. | outbreaks of serious mortality among cultured kuruma prawns (penaeus japonicus) with white spotted syndrome in the carapace occurred in the summer of 1993 in i-lan, taiwan. a swarming bacterium, strain swy, was isolated from the hepatopancreas of the moribund prawns using tryptic soy agar supplemented with 1% nacl and/or thiosulphate citrate bile salt sucrose agar. this strain was characterized and identified as vibrio alginolyticus on the basis of a number of biochemical tests. the swy strain w ... | 1996 | 8936369 |
| kinetics of adhesion of selected fish-pathogenic vibrio strains of skin mucus of gilt-head sea bream (sparus aurata l.). | the kinetics of adhesion of vibrio strains isolated from diseased fish to skin mucus of gilt-head sea bream was studied. a modified langmuir adsorption isotherm was calculated, and the results obtained indicate that the strains tested (vibrio alginolyticus dp1he4 and vibrio anguillarum-like dc12r8 and dc12r9) showed a saturation kinetics except for v. alginolyticus (can), which showed a proportional adsorption kinetics. the adhesive capability for skin mucus does not seem to be an essential viru ... | 1996 | 8837420 |
| antibacterial activity in the coelomocytes of the sea urchin paracentrotus lividus. | naturally present antibacterial activity directed against vibrio alginolyticus was demonstrated in coelomocytes lysate (cl) and cell-free coelomic fluid (cf) of the marine echinoderm paracentrotus lividus. kinetic analysis revealed that 5 min of contact was enough to induce significant bactericidal effect. maximum activity required 30 min of contact. nonsensitive to the effect of trypsin, the activity was almost completely suppressed by incubation with subtilisin. purified from cl by three succe ... | 1996 | 8829813 |
| cloning and sequencing of novel genes from vibrio alginolyticus that support the growth of k+ uptake-deficient mutant of escherichia coli. | novel genes that functionally complement the growth of k+ uptake-deficient mutant strain of escherichia coli tk420 have been cloned from the marine bacterium vibrio alginolyticus. the nucleotide sequence revealed three open reading frames. the second gene was homologous to proc gene and allowed the growth of proc-defective mutant strain of e. coli chi 342 in the absence of proline. the first and third genes, but not proc, were required for the growth of tk420 in a synthetic medium containing 10 ... | 1996 | 8982386 |
| association of aeromonas hydrophila and vibrio alginolyticus with larval mortalities of scallop (argopecten purpuratus) | a bacteriological study was carried out in a hatchery of argopecten purpuratus located in northern chile which had been affected by severe larval mortalities. the phenotypic characterization of the bacterial strains revealed that vibrio alginolyticus was the predominant species isolated in the majority of samples taken from the different units of the hatchery (microalgae, swimming larvae, seawater of larval culture tanks, and a reservoir tank of 50-μm filtered seawater). however, the bacterial p ... | 1996 | 8812601 |
| survey on the distribution of vibrionaceae at the seaport areas in taiwan, 1991-1994. | a monthly survey on the distribution of human-pathogenic vibrionaceae of the seawater from five principal harbors in taiwan was conducted by national quarantine service from july, 1991 to february, 1994. of the total 1,167 vibrionaceae isolates, strains of vibrio alginolyticus (449 strains) were the most frequently isolated, followed by vibrio parahaemolyticus (262) , aeromonas hydrophila (153), vibrio cholerae non-o1 (86), and vibrio vulnificus (67). none of vibrio cholerae o1 was isolated. the ... | 1996 | 10592802 |
| comparison of the growth and survival of larval turbot in the absence of culturable bacteria with those in the presence of vibrio anguillarum, vibrio alginolyticus, or a marine aeromonas sp. | larval turbot (scophthalmus maximus) were reared on rotifers (brachionus plicatilis) in the absence of culturable bacteria for up to 14 days and exhibited growth and high rates of survival (>55% in five experiments). low numbers of known bacteria were introduced into similar cultures by exposure of the rotifers to a suspension of bacteria prior to addition of rotifers to the larval cultures; vibrio anguillarum 91079 caused a highly significant decrease (p <0.01) in the proportion of survivors in ... | 1995 | 16535196 |
| n-terminal quarter part of tetracycline transporter from pacyc184 complements k+ uptake activity in k+ uptake-deficient mutants of escherichia coli and vibrio alginolyticus. | in an attempt to clone a gene encoding the k+ uptake system from vibrio alginolyticus, two plasmids, pkt2 and pkt4, were derived from pacyc184. these plasmids allowed the growth of k+ uptake-deficient mutant strains of escherichia coli tk420 and v. alginolyticus fs181 in a low k+ medium. the pkt2 and pkt4 had an insertion about 7 and 6 kb, respectively, from the genome of v. alginolyticus. we prepared deletion plasmids from both plasmids and found that the site of genes inserted in the two was n ... | 1995 | 8845802 |
| complicated suppurative otitis media in a greek diver due to a marine halophilic vibrio sp. | halophilic vibrios are distinct from non-cholera vibrios and have been recognized increasingly as potentially pathogenic bacteria in extraintestinal infections. a case of suppurative chronic otitis media in a greek diver with vibrio alginolyticus recovered from an ear drainage culture, is reported. the patient received appropriate antimicrobial therapy and her hearing improved significantly after a tympanoplasty type i was performed. the association of halophilic vibrio spp. infections with prol ... | 1995 | 8551126 |
| pathogenesis studies on vibrio alginolyticus in the grouper, epinephelus malabaricus, bloch et schneider. | a pathogenic vibrio, strain s3y was isolated from an outbreak of vibriosis in grouper (epinephelus malabaricus) fry in kaohsiung, southern taiwan in november 1991. it was identified as vibrio alginolyticus on the basis of a number of biochemical tests. the bacterium was therefore identified as vibrio, a gram-negative, straight rod, motile, oxidase and catalase positive, swarming on tsa plate, fermentative and produced yellow colonies on tcbs agar. it did not utilise sodium citrate, but it was ge ... | 1995 | 8559039 |
| detection of proteolytic bacteria in the upper respiratory tract flora of poultry. | proteolytic bacteria were readily demonstrated among the upper respiratory tract flora of poultry in two chicken flocks and two turkey flocks. between 20% and 50% of birds in each flock had highly proteolytic bacteria in the upper respiratory tract flora, and the amount of the bacterial flora determined to be highly proteolytic in any given bird ranged from none to a majority. highly proteolytic bacteria recovered included five species of staphylococci, as well as two gram-negative bacterial spe ... | 1995 | 8561749 |
| simultaneous measurement of bacterial flagellar rotation rate and swimming speed. | swimming speeds and flagellar rotation rates of individual free-swimming vibrio alginolyticus cells were measured simultaneously by laser dark-field microscopy at 25, 30, and 35 degrees c. a roughly linear relation between swimming speed and flagellar rotation rate was observed. the ratio of swimming speed to flagellar rotation rate was 0.113 microns, which indicated that a cell progressed by 7% of pitch of flagellar helix during one flagellar rotation. at each temperature, however, swimming spe ... | 1995 | 8580359 |
| acute gastroenteritis caused by vibrio alginolyticus in an immunocompetent patient. | 1995 | 8645804 | |
| the na(+)-translocating nadh:ubiquinone oxidoreductase from the marine bacterium vibrio alginolyticus contains fad but not fmn. | the na(+)-translocating nadh:ubiquinone oxidoreductase from vibrio alginolyticus was extracted from the bacterial membranes and purified by ion exchange chromatographic procedures. the enzyme catalyzed nadh oxidation by suitable electron acceptors, e.g. menadione, and the na+ and nadh-dependent reduction of ubiquinone-1. four dominant bands and a number of minor bands were visible on sds-page that could be part of the enzyme complex. flavin analyses indicated the presence of fad but no fmn in th ... | 1995 | 7649253 |
| high-speed rotation and speed stability of the sodium-driven flagellar motor in vibrio alginolyticus. | the na(+)-driven flagellar motor in vibrio alginolyticus rotates very fast. rotation of a single flagellum on a stuck cell was measured by laser darkfield microscopy with submillisecond temporal resolution. the rotation rate increased with increasing external concentration of nacl, and reached 1000 r.p.s. at 300 mm nacl. the na+ influx through the motor should determine the rotation period (tau) and affect the speed stability. fluctuation of the rotation period was analyzed at various rotation r ... | 1995 | 7643389 |
| a gene encoding a thermophilic alkaline serine proteinase from thermus sp. strain rt41a and its expression in escherichia coli. | the extreme thermophile thermus sp. strain rt41a produces an extracellular alkaline serine proteinase during growth. this enzyme is stable for more than 24 h at 70 degrees c and has a ph optimum of 8.0. the proteinase gene was identified using primers designed to amplify a region between two highly conserved amino acid motifs in subtilisin-like proteinases and the pcr product was used to identify a genomic fragment containing the gene. the amino acid sequence deduced from the rt41a gene containe ... | 1995 | 7551039 |
| predicted structure and possible ionmotive mechanism of the sodium-linked nadh-ubiquinone oxidoreductase of vibrio alginolyticus. | two groups have now published sequences of the six genes contained in the operon coding for the sodium-linked nadh-ubiquinone oxidoreductase of vibrio alginolyticus. sequence analyses indicate that this enzyme is unrelated to other known respiratory nadh dehydrogenases. a search for cofactor motifs suggests that the enzyme contains only one fad, a ferredoxin-type iron sulphur centre, and the nadh-binding site. these are all located on nqrf, a subunit that can be recognized as a new member of a l ... | 1995 | 7498478 |
| reexamination of tetrodotoxin production by bacteria. | vibrio alginolyticus has been reported as a good producer of tetrodotoxin (ttx), but the toxin extracted from this bacterium did not react to the monoclonal antibody against ttx. surprisingly, chromatographic analyses detected high ttx peaks for polypeptone and yeast extracts used as medium materials, which were, as expected, all negative by the mouse bioassay. these results may require us to revise the bacterial production of ttx. | 1995 | 7574655 |
| molecular analysis of an extracellular protease gene from vibrio parahaemolyticus. | the structural gene prtvp encoding the extracellular protease of vibrio parahaemolyticus strain 93 was cloned in escherichia coli and sequenced. the cloned dna fragment contained a 1761 bp orf encoding a 587 amino acid protein. the deduced polypeptide is composed of a 25 amino acid signal peptide and a 562 amino acid extracellular polypeptide with a calculated molecular mass of 63,156 da. protease analysis using a gelatin-containing sds-polyacrylamide gel detected the presence of a 62 kda protea ... | 1995 | 7582017 |
| three aspartic residues in membrane-spanning regions of na+/h+ antiporter from vibrio alginolyticus play a role in the activity of the carrier. | the na+/h+ antiporter gene from vibrio alginolyticus restores the growth of an nhaa-defective strain of escherichia coli, nm81, in a high nacl medium (nakamura, t., komano, y., itaya, e., tsukamoto, k., tsuchiya, t. and unemoto, t. (1994) biochim. biophys. acta 1190, 465-468). this gene, named nhaav, allowed the nhaa-defective e. coli strains, nm81(delta nhaa) and rs1 (delta nhaa, chaa-), to extrude na+ at alkaline ph. the extrusion of na+ occurred against its chemical gradient in the presence o ... | 1995 | 7619834 |
| isolation of the polar and lateral flagellum-defective mutants in vibrio alginolyticus and identification of their flagellar driving energy sources. | vibrio alginolyticus has two types of flagella (polar and lateral) in one cell. we isolated mutants with only a polar flagellum (pof+ laf-) or only lateral flagella (pof- laf+). using these mutants, we demonstrated that the energy sources of the lateral and polar flagellar motors in v. alginolyticus are h+ and na+ motive forces, respectively, as in the related species v. parahaemolyticus. | 1995 | 7665498 |
| sequencing and the alignment of structural genes in the nqr operon encoding the na(+)-translocating nadh-quinone reductase from vibrio alginolyticus. | we previously cloned a part of nqr operon encoding the na(+)-translocating nadh-quinone reductase (nqr) from the marine vibrio alginolyticus [hayashi et al., febs lett. 356 (1994) 330-332]. from its nucleotide sequences, four consecutive open reading frames (orf) encoding the gamma-subunit (27.7 kda), two unidentified orfs of 22.6 kda and 21.5 kda, and the beta-subunit (45.3 kda) were recognized. the gene encoding the alpha-subunit was located upstream, and together with the recent report by bea ... | 1995 | 7729558 |
| self extraction of foreign body lodged in subtarsal sulcus (a simple first-aid measure). | 1995 | 1236449 | |
| the chronic patient: perspectives from the 29th institute on hospital & community psychiatry. the role of the psychiatrist [proceedings]. | 1994 | 201557 | |
| cloning of the na(+)-translocating nadh-quinone reductase gene from the marine bacterium vibrio alginolyticus and the expression of the beta-subunit in escherichia coli. | the na(+)-translocating nadh-quinone reductase purified from the marine bacterium vibrio alginolyticus is composed of three subunits, alpha, beta and gamma. from the n-terminal amino acid sequences of each subunit and its polypeptide fragment obtained by partial digestion with v8 protease, oligonucleotides corresponding to forward and reverse primers for each gene (nqr a, b and c) encoding the alpha, beta and gamma subunit, respectively, were synthesized. using these primers, a part of each gene ... | 1994 | 7805866 |
| evaluation of hawaiian green turtles (chelonia mydas) for potential pathogens associated with fibropapillomas. | thirty-two juvenile green turtles (chelonia mydas) were captured alive in kaneohe bay, island of oahu, hawaii, during september 1991. ten of the turtles sampled were afflicted with green turtle fibropapillomatosis (gtfp) in varying degrees of severity. virus isolation attempts were negative in all individuals. using nasopharyngeal and cloacal swabs, we isolated 28 gram negative bacteria, five gram positive cocci, bacillus spp., and diphtheroids. the most common isolates included pseudomonas fluo ... | 1994 | 8151829 |
| [extraintestinal infections caused by vibrio parahaemolyticus and vibrio alginolyticus at the county of funen 1987-1992]. | over a six-year period we detected 30 clinical infections caused by halophilic vibrios in a restricted geographical area. vibrio parahaemolyticus infections were found in 13 patients (three with wound infections, ten with ear infections), and vibrio alginolyticus infections in 17 patients, all of whom had ear infections. from 1987 to 1990, infections caused by marine vibrios were found in at most four cases annually, in 1992 in six instances, whereas over a five-month period in 1991 we experienc ... | 1994 | 7941065 |
| cloning and sequencing of four structural genes for the na(+)-translocating nadh-ubiquinone oxidoreductase of vibrio alginolyticus. | oligonucleotide probes based on the n-terminal amino acid sequences of the nqra and nqrc subunits were used to clone genes for the na(+)-dependent nadh-ubiquinone oxidoreductase complex from vibrio alginolyticus. four consecutive orfs were identified encoding subunit proteins of 48.6, 46.8, 27.7 and 22.6 kda, respectively (nqra-d). a further orf, showing 71% homology to the bola protein of escherichia coli, was located upstream. from sequence comparisons, we conclude that the na(+)-dependent nad ... | 1994 | 7805867 |
| cloning and sequencing of a k+ transport gene (trk a) from the marine bacterium vibrio alginolyticus. | a gene has been cloned from the marine bacterium vibrio alginolyticus that functionally complements a mutant strain of escherichia coli, tk420, defective in k+ transport genes (kdpabc, trkd, trka). the cloned vibrio gene allowed tk420 to grow in a synthetic medium containing less than 10 mm k+ and concomitantly led to an increase in k+ uptake activity. the nucleotide sequence of the cloned fragment revealed an open reading frame, which encodes a protein with a predicted 458 amino acid sequence a ... | 1994 | 7948029 |
| cloning and nucleotide sequence analysis of the colh gene from clostridium histolyticum encoding a collagenase and a gelatinase. | the colh gene encoding a collagenase was cloned from clostridium histolyticum jcm 1403. nucleotide sequencing showed a major open reading frame encoding a 116-kda protein of 1,021 amino acid residues. the deduced amino acid sequence contains a putative signal sequence and a zinc metalloprotease consensus sequence, hexxh. a 116-kda collagenase and a 98-kda gelatinase were copurified from culture supernatants of c. histolyticum. while the former degraded both native and denatured collagen, the lat ... | 1994 | 7961400 |
| potentially pathogenic vibrios associated with mussels from a tropical region on the atlantic coast of brazil. | mussels (perna perna) harvested on the coast of ubatuba, in three different stations in the state of são paulo, brazil, were examined for vibrio spp. over a 1 year period. the ranges of most probable number (mpn 100 g-1) were: vibrio alginolyticus (< 3-24,000), v. parahaemolyticus (< 3-24,000), v. fluvialis (< 3-1100), v. cholerae non-o1 (< 3-23), v. furnissii (< 3-30), v. mimicus (< 3-9) and v. vulnificus (< 3-3). the highest incidence was observed for v. alginolyticus (92-100%), followed by v. ... | 1994 | 7989253 |
| regulation of extracellular copper-binding proteins in copper-resistant and copper-sensitive mutants of vibrio alginolyticus. | extracellular proteins of wild-type vibrio alginolyticus were compared with those of copper-resistant and copper-sensitive mutants. one copper-resistant mutant (cu40b3) constitutively produced an extracellular protein with the same apparent molecular mass (21 kda) and chromatographic behavior as copper-binding protein (cubp), a copper-induced supernatant protein which has been implicated in copper detoxification in wild-type v. alginolyticus. copper-sensitive v. alginolyticus mutants displayed a ... | 1994 | 8031076 |
| cloning and nucleotide sequence of the carboxynorspermidine decarboxylase gene from vibrio alginolyticus. | the gene (nspc) encoding carboxynorspermidine decarboxylase (cans dc), the last enzyme in norspermidine biosynthesis, in vibrio alginolyticus was isolated by immuno-screening and its complete nucleotide sequence was determined. sequence analysis of the subcloned fragment (2.0 kb) revealed an orf of 1131 bp encoding a protein of 377 amino acids with a calculated molecular mass of 42,008 da. the sequence of 20 n-terminal amino acids of purified cans dc was found to be identical to that predicted f ... | 1994 | 7812450 |
| cloning and sequencing of an na+/h+ antiporter gene from the marine bacterium vibrio alginolyticus. | a gene has been cloned from a dna library from the marine bacterium vibrio alginolyticus that functionally complements a mutant strain of escherichia coli, nm81, defective in an na+/h+ antiporter (nhaa). the cloned vibrio gene restored nm81 to grow in a medium containing 0.5 m nacl at ph 7.5 and concomitantly led to an increase in na+/h+ antiport activity. the nucleotide sequence of the cloned fragment revealed an open reading frame, which encodes a protein with a predicted 383 amino acid sequen ... | 1994 | 8142450 |
| purification and characterization of clostridium perfringens 120-kilodalton collagenase and nucleotide sequence of the corresponding gene. | clostridium perfringens type c ncib 10662 produced various gelatinolytic enzymes with molecular masses ranging from approximately 120 to approximately 80 kda. a 120-kda gelatinolytic enzyme was present in the largest quantity in the culture supernatant, and this enzyme was purified to homogeneity on the basis of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the purified enzyme was identified as the major collagenase of the organism, and it cleaved typical collagenase substrates such ... | 1994 | 8282691 |
| responses of diverse heterotrophic bacteria to elevated copper concentrations. | the influence of copper on the growth of escherichia coli, pseudomonas aeruginosa, bacillus cereus, bacillus subtilis, vibrio parahaemolyticus, vibrio alginolyticus (three strains), and an unidentified vibrio sp. was examined in batch cultures. the effects of copper at micromolar concentrations varied from undetectable to complete growth inhibition. each strain was able to recover from a growth lag observed after copper addition at a characteristic concentration. copper concentrations that allow ... | 1994 | 8069784 |
| excitatory signaling in bacterial probed by caged chemoeffectors. | chemotactic excitation responses to caged ligand photorelease of rapidly swimming bacteria that reverse (vibrio alginolyticus) or tumble (escherichia coli and salmonella typhimurium) have been measured by computer. mutants were used to assess the effects of abnormal motility behavior upon signal processing times and test feasibility of kinetic analyses of the signaling pathway in intact bacteria. n-1-(2-nitrophenyl)ethoxycarbonyl-l-serine and 2-hydroxyphenyl 1-(2-nitrophenyl) ethyl phosphate wer ... | 1993 | 8312476 |
| [otitis externa caused by vibrio alginolyticus: description of 4 cases]. | vibrio alginolyticus is a microorganism of marine environment which occasionally may cause infections on man. we described four patients with external otitis who had prolonged contact with sea water presented itch and seropurulent fluid. v. alginolyticus was isolated by pure culture from ear fluid of three of them and associated to v. fluvialis in another. the administration of ciprofloxacin in three cases, and ceftazidime in the other succeeded in the remission of the symptoms. | 1993 | 8471288 |
| sequence analysis of scra and scrb from streptococcus sobrinus 6715. | the complete nucleotide sequences of streptococcus sobrinus 6715 scra and scrb, which encode sucrose-specific enzyme ii of the phosphoenolpyruvate-dependent phosphotransferase system and sucrose-6-phosphate hydrolase, respectively, have been determined. these two genes were transcribed divergently, and the initiation codons of the two open reading frames were 192 bp apart. the transcriptional initiation sites were determined by primer extension analysis, and the putative promoter regions of thes ... | 1993 | 8500898 |
| extraintestinal infections caused by vibrio parahaemolyticus and vibrio alginolyticus in a danish county, 1987-1992. | over a 6-year period we detected 30 clinical infections caused by halophilic vibrios in a restricted geographical area. vibrio parahaemolyticus infections were found in 13 patients (3 with wound infections, 10 with ear infections), and vibrio alginolyticus infections in 17 patients, all of whom had ear infections. from 1987 to 1990, infections caused by marine vibrios were found in at most 4 cases annually, in 1992 in 6 instances, whereas in a 5-month period in 1991 we experienced 15 cases of ex ... | 1993 | 8052813 |
| purification and properties of cytochrome bo-type ubiquinol oxidase from a marine bacterium vibrio alginolyticus. | ubiquinol oxidase was extracted from membranes of a marine bacterium vibrio alginolyticus with a nonionic detergent liponox dch and was purified about 130-fold by deae-sephacel, deae-5pw and sephacryl s-300. the purified ubiquinol oxidase was composed of three subunits with apparent m(r) of 79, 36 and 13 kda on sds-polyacrylamide gel electrophoresis. the oxidase contained cytochrome b, cytochrome o and copper atoms. the presence of heme o was confirmed by reverse-phase hplc analysis. ubiquinol-1 ... | 1993 | 8443214 |
| a case of vibrio alginolyticus bacteremia and probable sphenoiditis following a dive in the sea. | 1993 | 8399897 | |
| respiration-driven na+ pump of the marine vibrio is encoded by chromosomal dna. | a plasmid-cured strain of the marine vibrio alginolyticus 138-2 retains a respiration-driven na+ pump. examinations of several strains of v. alginolyticus and v. parahaemolyticus revealed that these murine vibrio always possessed the respiration-driven na+ pump irrespective of the presence or absence of plasmids. these results strongly suggested that the genes for the na+ pump were encoded by chromosomal dna. | 1993 | 8220320 |
| na(+)-translocating nadh-quinone reductase of marine and halophilic bacteria. | the respiratory chain of marine and moderately halophilic bacteria requires na+ for maximum activity, and the site of na(+)-dependent activation is located in the nadh-quinone reductase segment. the na(+)-dependent nadh-quinone reductase purified from marine bacterium vibrio alginolyticus is composed of three subunits, alpha, beta, and gamma, with apparent m(r) of 52, 46, and 32 kda, respectively. the fad-containing beta-subunit reacts with nadh and reduces ubiquinone-1 (q-1) by a one-electron t ... | 1993 | 8226720 |
| [the isolation of vibrio alginolyticus bacteriophage]. | we identified 4 bacteriophages of v. alginolyticus in 29 ones, which were first isolated from seafood. according to their character of the plaques, they were classified into two kinds: one plaque was clear, the other was opaque. the size of these plaques were different and their diameters are 0.5-3.0mm. by electron microscopy observation, they could be classified into two kinds; one has a long axie and hexagemal head, and a thin-long tail, the other has an equal axie hexagenal head and a very sh ... | 1993 | 8256441 |
| isolation, characterization and sequence analysis of the scrk gene encoding fructokinase of streptococcus mutans. | a gene encoding an atp-dependent fructokinase from streptococcus mutans gs-5 was identified within a 2 kb dna fragment immediately downstream from the scra gene. the gene cloned in escherichia coli also expressed mannokinase activity. insertional inactivation of this gene in s. mutans markedly decreased both fructokinase and mannokinase activities. nucleotide sequence analysis of the 2 kb fragment revealed an orf starting 199 bp downstream from the scra gene, preceded by potential ribosome-bindi ... | 1993 | 8336109 |
| [otitis media due to vibrio alginolyticus: the risks of the mediterranean sea]. | 1993 | 8256965 | |
| growth, copper-tolerant cells, and extracellular protein production in copper-stressed chemostat cultures of vibrio alginolyticus. | the influence of elevated copper concentrations on cell numbers and extracellular protein production was investigated in chemostat cultures of vibrio alginolyticus. high (20 microm) copper in the medium reservoir resulted in a dramatic drop in cell numbers which was overcome with time. the copper-stressed cultures established a new equilibrium cell concentration slightly (ca. 20%) lower than control cultures. copper-stressed chemostat populations contained an increased number of copper-resistant ... | 1993 | 8439167 |
| vibrio alginolyticus wound infection after exposure to sea water in an air crash. | 1993 | 8359172 | |
| [isolation of vibrio alginolyticus in 2 cases of otitis externa]. | 1993 | 8499526 | |
| electrochemical prevention of marine biofouling with a carbon-chloroprene sheet. | a carbon-chloroprene sheet (ccs) electrode was used for the electrochemical disinfection of the marine gram-negative bacterium vibrio alginolyticus. when the electrode was incubated in seawater containing 10 cells per ml for 90 min, the amount of adsorbed cells was 4.5 x 10 cells per cm. when a potential of 1.2 v versus a saturated calomel electrode was applied to the ccs for 20 min, 67% of adsorbed cells were killed. this disinfection was due to the direct electrochemical oxidation of cells and ... | 1993 | 16349089 |
| occurrence of vibrio alginolyticus in ligurian coast rock pools (tyrrhenian sea, italy) and its association with the copepod tigriopus fulvus (fisher 1860). | a study of heterotrophic bacteria and vibrios adhering to the copepod tigriopus fulvus, which lives in ligurian coast rock pools (tyrrhenian sea), was carried out from november 1990 to october 1991. heterotrophic bacteria, which were always found both free in the water and bound to the t. fulvus organisms, showed a correlation with water temperature and salinity. vibrio alginolyticus was found free in the water and bound to t. fulvus surfaces during the warmest months. temperature is the main fa ... | 1993 | 16348971 |
| structural gene and complete amino acid sequence of vibrio alginolyticus collagenase. | the dna encoding the collagenase of vibrio alginolyticus was cloned, and its complete nucleotide sequence was determined. when the cloned gene was ligated to puc18, the escherichia coli expression vector, bacteria carrying the gene exhibited both collagenase antigen and collagenase activity. the open reading frame from the atg initiation codon was 2442 bp in length for the collagenase structural gene. the amino acid sequence, deduced from the nucleotide sequence, revealed that the mature collage ... | 1992 | 1311172 |
| roles of k+ and na+ in ph homeostasis and growth of the marine bacterium vibrio alginolyticus. | the marine bacterium vibrio alginolyticus, containing 470 mm-k+ and 70 mm-na+ inside its cells, was able to regulate the cytoplasmic ph (ph(in)) in the narrow range 7.6-7.8 over the external ph (ph(out)) range 6.0-9.0 in the presence of 400 mm-na+ and 10 mm-k+. in the absence of external k+, however, phin was regulated only at alkaline ph(out) values above 7.6. when the cells were incubated in the presence of unusually high k+ (400 mm) and 4 mm na+, the ph(in) was regulated only at acidic ph(out ... | 1992 | 1326594 |
| catabolite repression of the h(+)-translocating atpase in vibrio parahaemolyticus. | cells of vibrio parahaemolyticus grown in the presence of glucose showed reduced (by about 40%) oxidative phosphorylation. with this observation as a basis, we examined the effect of glucose on the level of h(+)-translocating atpase. the addition of glucose to the growth medium reduced the specific activity and the amount of the h(+)-translocating atpase in membrane vesicles of v. parahaemolyticus. these reductions were reversed by adding cyclic amp (camp) to the growth medium. we cloned some pa ... | 1992 | 1328162 |
| towards a phylogeny of the genus vibrio based on 16s rrna sequences. | the inter- and intrageneric relationships of the genus vibrio were investigated by performing a comparative analysis of the 16s rrnas of 10 species, including four pathogenic representatives. the results of immunological and 5s rrna studies were confirmed in that the genus is a neighboring taxon of the family enterobacteriaceae. with regard to the intrageneric structure, vibrio alginolyticus, vibrio campbellii, vibrio natriegens, vibrio harveyi, vibrio proteolyticus, vibrio parahaemolyticus, and ... | 1992 | 1371064 |
| variations in rrna content of marine vibrio spp. during starvation-survival and recovery. | the degree and temporal context of variations in ribosome content during nutrient starvation of two copiotrophic marine bacteria, vibrio alginolyticus and vibrio furnissii, have been examined. the organisms were starved either by nutritional shift-down or by consumption of limiting nutrients resulting from growth into stationary phase. measurements of the amount of hybridization to 16s rrna-specific probes revealed that the cells retained between 10 and 26% of their original rrna content after 1 ... | 1992 | 1371659 |
| fimbriation, capsulation, and iron-scavenging systems of klebsiella strains associated with human urinary tract infection. | thirty-two strains of klebsiella pneumoniae and seven strains of klebsiella oxytoca isolated from urinary tract infections in elderly adults were analyzed for capsular antigens, iron-scavenging systems, and fimbriation. all strains were capsulated. twenty-seven different k antigens were identified among the strains, with no particular antigen dominating. all strains produced the iron-scavenging system enterochelin as analyzed by bioassay and dna hybridization. in contrast, the aerobactin iron-se ... | 1992 | 1347287 |
| [h2-receptor antagonist-refractory ulcer--its pathophysiology and role of proton-pump inhibitors]. | among h2-receptor antagonist (h2ra)-refractory ulcers, non-responders that did not heal after 5 months therapy had high intraluminal ph in the basal condition and high sensitivity to inhibition of acid secretion by h2ra but possessed gastric mucosa to generate less prostaglandins. combination therapy of pge1-analogue with h2ra healed these ulcers by 60%. proton-pump inhibitor (ppi) exerted a complete inhibition of acid secretion in these patients and the rate of healing was 88%. helicobacter pyl ... | 1992 | 1347325 |
| amiloride at ph 7.0 inhibits the na(+)-driven flagellar motors of vibrio alginolyticus but allows cell growth. | amiloride, a specific inhibitor for the na(+)-driven flagellar motors of alkalophilic bacillus, is known to inhibit secondarily the growth of alkalophiles. the motility of a marine vibrio, v. alginolyticus, was almost completely inhibited by 2 mm amiloride either at ph 7.0 or 8.5. we found that this concentration of amiloride inhibited the cell growth completely at ph 8.5 but only slightly at ph 7.0. kinetic analysis of the inhibition of motility by amiloride at ph 7.0 showed that the inhibition ... | 1992 | 1459240 |
| ultrastructural alterations of brain cortex in rat following intraperitoneal administration of mercuric chloride. | intraperitoneal administration of a single dose (6 mg/kg body weight) of mercuric chloride (hgcl2) results in ultrastructural changes in brain cortex of the rats. 18 hours after administration of hgcl2 the accumulation of dense deposits of mercury in nerve and glial cell cytoplasm was observed. the quantity of microglia in neuropil was also increased in this experimental group. we postulate that these cerebral macrophages can play an important role in the process of intoxication, too. 5 days aft ... | 1992 | 1479187 |
| vibrio alginolyticus ("achromobacter") collagenase: biosynthesis, function and application. | bacterial collagenase from aerobic non-pathogenic vibrio alginolyticus chemovar iophagus ("achromobacter" collagenase, ec 3.4.24.08) is an inducible extracellular metallo-proteinase. production of vibrio collagenase is induced specifically by collagen or by its macromolecular fragments. on the cell surface is expressed a specific receptor recognizing collagen structure. the study of natural inducers led to synthetic peptides with inducing properties. vibrio collagenase cleaves collagen helical c ... | 1992 | 1480012 |
| development of monoclonal antibodies that identify vibrio species commonly isolated from infections of humans, fish, and shellfish. | monoclonal antibodies (mabs) against vibrio species that infect humans, fish, and shellfish were developed for application in rapid identifications. the pathogens included vibrio alginolyticus, v. anguillarum, v. carchariae, v. cholerae, v. damsela, v. furnissii, v. harveyi, v. ordalii, v. parahaemolyticus, and v. vulnificus. three types of mabs were selected. the first important group included mabs that reacted with only a single species. a second group comprised a number of mabs that reacted w ... | 1992 | 1482190 |
| purification and characterization of l-2,4-diaminobutyrate decarboxylase from acinetobacter calcoaceticus. | acinetobacter calcoaceticus atcc 23055 produces a large amount of 1,3-diaminopropane under normal growth conditions. the enzyme responsible, l-2,4-diaminobutyrate (daba) decarboxylase (ec 4.1.1.-), was purified to electrophoretic homogeneity from this bacterium. the native enzyme had an m(r) of approximately 108,000, with a pi of 5.0, and was a dimer composed of identical or nearly identical subunits with apparent m(r) 53,000. the enzyme showed hyperbolic kinetics with a km of 1.59 mm for daba a ... | 1992 | 1512577 |
| properties of respiratory chain-linked na(+)-independent nadh-quinone reductase in a marine vibrio alginolyticus. | the respiratory chain of a marine vibrio alginolyticus contains two types of nadh-quinone reductase (nqr): one is an na(+)-dependent nqr functioning as an na+ pump (nqr-1) and the other is an na(+)-independent nqr (nqr-2). nqr-2 was purified about 55-fold from the membrane of mutant nap-1 which is devoid of nqr-1, and its properties were compared with those of nqr-1. in contrast to nqr-1, the purified nqr-2 does not require any salts for activity and is not inhibited by up to 0.4 m salts. the op ... | 1992 | 1543699 |
| [changes in centrally released arginine vasopressin by stimulation of the medullary reticular formation]. | it has been reported that after 40 minutes of stimulation of the medullary reticular formation (morf), widespread significant increase by 1.4% to 2.8% in brain water content occurs in white matter of the injured hemisphere. recent studies indicate that centrally released arginine vasopressin (avp) influences water permeability of the brain in both normal and pathological conditions. the present study was carried out to clarify the effect of electrical stimulation of morf on centrally released av ... | 1992 | 1562384 |
| the highest gene concentrations in the human genome are in telomeric bands of metaphase chromosomes. | chromosome in situ suppression hybridization has been carried out on human metaphase chromosomes to localize the g+c-richest human dna fraction (which only represents 3.5% of the genome), as isolated by preparative equilibrium centrifugation in cs2so4/3,6-bis(acetatomercurimethyl)-1,4-dioxane density gradient. this fraction essentially corresponds to isochore family h3. the rationale for carrying out this experiment is that this isochore family has, by far, the highest gene concentration, the hi ... | 1992 | 1594593 |
| characteristic differences in the mode of quinone reduction and stability between energy-coupled and -uncoupled nadh-quinone reductases from bacterial respiratory chain. | bacterial respiratory chain has two types of nadh-quinone reductase (nqr): one is energy-coupled (type-1) and the other had no energy-transducing capacity, that is, energy-uncoupled (type-2). each of the nadh-reacting flavoprotein subunits of nqr-1 from escherichia coli and the marine vibrio alginolyticus reduced quinone to semiquinone radicals by the one-electron transfer pathway and was very sensitive to preincubation with nadh. on the other hand, the nqr-2 from these bacteria reduced quinone ... | 1992 | 1628743 |
| expression of bacillus amyloliquefaciens amylase and vibrio alginolyticus protease a fusion genes. | previously we reported [deane, s. m., maharaj, r., robb, f. t. & woods, d. r. (1987) journal of general microbiology 133, 2295-2302] that the production of a vibrio alginolyticus sds-resistant alkaline serine protease (pro a) cloned in escherichia coli was characterized by a 12 h delay between the synthesis of an inactive precursor and secretion of active pro a. replacement of the v. alginolyticus promoter region by the alpha-amylase promoter region from bacillus amyloliquefaciens resulted in th ... | 1992 | 1373436 |
| mapping of antigenic sites to monoclonal antibodies on the primary structure of the f1-atpase beta subunit from escherichia coli: concealed amino-terminal region of the subunit in the f1. | to analyze relationships between the ternary and primary structures of the beta subunit of escherichia coli f1 atpase, we prepared two monoclonal antibodies beta 12 and beta 31 against the beta peptide. these antibodies bind to the beta subunit but do not bind to the f1 atpase, resulting in no inhibition of the atpase activities. several different portions of the beta subunit peptide were prepared by constructing expression plasmids carrying the corresponding dna segment of the beta subunit gene ... | 1992 | 1373594 |
| incidence of toxigenic vibrios in foods available in taiwan. | a total of 1088 vibrios and related species were isolated from seafood and aquacultured foods available in taiwan. they were identified as vibrio alginolyticus, v. cholerae, v. fluvialis i, v. fluvialis ii, v. parahaemolyticus, v. mimicus, aeromonas caviae, a. hydrophila, a. sobria and other species. incidence of these vibrio and aeromonas species in these foods was high. vibrio parahaemolyticus was frequently found in seawater and in foods of freshwater origin. the vibrio isolates were examined ... | 1992 | 1399913 |
| characterization of a vibrio cholerae virulence factor homologous to the family of tonb-dependent proteins. | irga is an iron-regulated virulence factor for infection in an animal model with classical vibrio cholerae strain 0395. we detected gene sequences hybridizing to irga at high stringency in clinical isolates in addition to 0395, including another classical strain of v. cholerae, three v. cholerae strains of the el tor biotype, three non-o1 isolates of v. cholerae, and individual isolates of vibrio parahaemolyticus, vibrio fluvialis, and vibrio alginolyticus. no hybridization to irga was seen with ... | 1992 | 1406279 |
| [vibrio alginolyticus in clinical specimens]. | 1992 | 1450271 | |
| [membrane atpase of vibrio alginolyticus. ion transport activity and homology with f0f1-atpase from e. coli]. | f0f1-atpase has been isolated from the marine alkali-resistant bacterium vibrio alginolyticus. the enzyme subunits cross-reacted with antibodies against subunits alpha, beta, gamma, epsilon, and b of e. coli atpase. the purified atpase was reconstituted into liposomes effecting an atp-dependent uptake of h+. proton transport was inhibited by the atpase blockers dccd, triphenyltin, and venturicidin. na+ ions had no effect on atp-dependent proton transport. no atp-dependent transport of na+ was de ... | 1992 | 1457596 |
| prediction of transmembrane topology of f0 proteins from escherichia coli f1f0 atp synthase using variational and hydrophobic moment analyses. | the a subunit, a membrane protein from the e. coli f1f0 atp synthase has been examined by fourier analysis of hydrophobicity and of amino-acid residue variation. the amino-acid sequences of homologous subunits from vibrio alginolyticus, saccharomyces cerevisiae, neurospora crassa, aspergillus nidulans, schizosaccharomyces pombe and candida parapsilosis were used in the variability analysis. by fourier analysis of sequence variation, two transmembrane helices are predicted to have one face in con ... | 1992 | 1445940 |
| interleukin-1 receptor antagonist protein inhibits interleukin-8 expression in lipopolysaccharide-stimulated human whole blood. | interleukin-8 (il-8) is a neutrophil and lymphocyte chemoattractant and activator that may play an important role in mediating events at sites of inflammation. il-8 is produced by many cell types in response to a variety of inducers, including interleukin-1 (il-1). studies were conducted to address the question of whether an inhibitor of il-1 action, il-1 receptor antagonist protein (irap), would suppress il-8 production. lipopolysaccharide (lps)-stimulated human whole blood was used as an ex vi ... | 1992 | 1533750 |
| [changes of cholesterol and triglycerides in suckling infants: relationship with genetic markers of beta-lipoproteins]. | 1992 | 195192 | |
| [serum lipids in down's syndrome]. | in 28 patients with down syndrome and in 28 controls matched for age and sex serum lipoproteins and their main lipid constituents were determined. both groups comprised subjects living in the same institutions for mentally retarded no significant differences were found between the two groups in total serum lipids, triglycerides, total cholesterol and phospholipid levels and in the proportion of esterified cholesterol and beta-lipoproteins. no characteristic pattern of alpha and beta lipoproteins ... | 1992 | 183162 |
| letter: the critical coronary disease patient. | 1992 | 1130748 | |
| purification and some properties of carboxynorspermidine synthase participating in a novel biosynthetic pathway for norspermidine in vibrio alginolyticus. | carboxynorspermidine synthase, mediates the nicotinamide-nucleotide-linked reduction of the schiff base h2n(ch2)3n = chch2ch(nh2)cooh. this is formed from l-aspartic beta-semialdehyde (asa) and 1,3-diaminopropane (dap) and is reduced to carboxynorspermidine [h2n(ch2)3nh(ch2)2ch(nh2)cooh], an intermediate in the novel pathway for norspermidine (nspd) biosynthesis. the enzyme was purified to apparent homogeneity from vibrio alginolyticus and characterized. the overall purification was about 1800-f ... | 1991 | 1955861 |