Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| evaluation of prostaglandins as mediators of tubuloglomerular feedback. | orthograde and retrograde microperfusion experiments were conducted in sprague-dawley rats to evaluate the participation of vasoconstrictive eicosanoids as mediators of tubuloglomerular feedback (tgf) signals. retrograde perfusion with 160 microm arachidonic acid (aa) added to a hypotonic solution enhanced the stop-flow pressure (sfp) feedback responses compared with those obtained with the control hypotonic solution (delta sfp, 1.6 +/- 0.4 vs. 10.1 +/- 0.7 mmhg with aa). blockade of thromboxane ... | 1988 | 3364575 |
| polarity reversal of inside-out thyroid follicles cultured within collagen gel: structure of the junctions assessed by freeze-fracture and lanthanum permeability. | the organization of tight junctional complexes (tjs) was studied in cultured porcine thyroid cells during the inversion of polarity induced by collagen-embedding of inside-out follicles, using freeze-fracture replicas and lanthanum penetration. during the early steps of polarity reversal, freeze-fractures showed that tjs generally persisted. they increased in width and progressively branched out into the basolateral surfaces, towards the basal pole. later, the number of tj strands decreased and ... | 1988 | 3390623 |
| the processing of asparagine-linked oligosaccharides in ht-29 cells is a function of their state of enterocytic differentiation. an accumulation of man9,8-glcnac2-asn species is indicative of an impaired n-glycan trimming in undifferentiated cells. | studies on the regulation of the enterocytic differentiation of the human colon cancer cell line ht-29, which is differentiated in the absence (glc-) but not in the presence of glucose (glc+), have recently shown that the post-translational processing of sucrase-isomaltase and particularly its glycosylation vary as a function of cell differentiation (trugnan g., rousset, m., chantret, i., barbat, a., and zweibaum, a. (1987) j. cell biol. 104, 1199-1205). other studies indicate that in undifferen ... | 1988 | 3360773 |
| adhesion of aeromonas salmonicida strains associated with net electrostatic charges of host tissue cells. | the adhesion of aeromonas salmonicida, the pathogenic bacterium of fish furunculosis in salmon and trout, to the surface of host tissue cells was investigated with two fish tissue culture cell lines (rtg-2 cells from rainbow trout, salmo gairdneri, and chse-214 cells from chinook salmon, oncorhynchus tshawytscha) and four a. salmonicida strains. bacterial cells of pathogenic strains were highly adhesive to rtg-2 and chse-214 cells and were negatively charged in the net electrostatic charges, as ... | 1987 | 3818093 |
| residual nitrobenzylthioinosine-resistant nucleoside transport in a transport mutant (ae1) of s49 murine t-lymphoma cells. | the uptake of various nucleosides by s49 mouse t-lymphoma cells and that by a single-step nucleoside transport-defective mutant thereof (ae1) were compared. residual nucleoside entry into ae1 cells occurred via two routes, nonmediated permeation and saturable, non-concentrative transport with broad substrate specificity and a michaelis-menten constant approximating that for thymidine transport in wild-type cells. however, in contrast to nucleoside transport in wild-type cells, residual nucleosid ... | 1987 | 3494190 |
| enhancement of neutrophil-mediated phagocytosis by human granulocyte-macrophage colony-stimulating factor demonstrated using a novel mathematical model. | a mathematical model is presented which may be applied to describe and analyse data from microscopic phagocytosis assays. the method has been used to investigate the phagocytosis of opsonized yeast by peripheral blood neutrophils treated with purified recombinant human granulocyte-macrophage colony-stimulating factor (rh gm-csf) in vitro. under limiting conditions of serum opsonization, rh gm-csf decreased the proportion of non-phagocytic cells and increased the mean number of ingested yeast per ... | 1987 | 3500121 |
| [a new method for the etiological diagnosis of human scabies]. | 1987 | 3504153 | |
| application of transmission electron microscopy to human tumor diagnosis: an historical perspective. | 1987 | 3322513 | |
| identification of major common extracellular proteins secreted by aeromonas salmonicida strains isolated from diseased fish. | ten different strains of aeromonas salmonicida that were isolated from diseased fish were grown under identical conditions (24 h at 25 degree c) in 3% (wt/vol) tryptone soya broth medium supplemented with vitamins and inorganic ions. in each case the extracellular proteins that were formed were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and it was found that there were two significant common components, one with a molecular weight of 70,000 and the other with a weight ... | 1987 | 3555336 |
| on the increased retention volume of human haemoglobin in high-performance gel filtration. | 1987 | 3429527 | |
| amelioration of the ischemic damage of the dog kidney subjected to 90 minutes of warm ischemia by lidocaine pretreatment and low-dose dopamine infusion on revascularization. | 1987 | 3303853 | |
| purification of infectious canine parvovirus from cell culture by affinity chromatography with monoclonal antibodies. | immuno affinity chromatography with virus neutralizing monoclonal antibodies, directed to the haemagglutinating protein of canine parvovirus (cpv) was used to purify and concentrate cpv from infected cell culture. the procedure was monitored by testing the respective fractions in an infectivity titration system, in an elisa, in a haemagglutination assay and by negative contrast electron microscopy to quantify cpv or cpv antigen. the degree of purification was further estimated by testing the fra ... | 1987 | 3294877 |
| further improvement of high-speed nmr flow-velocity measurement using a differential phase-encoding technique. | an nmr imaging technique applicable to high-velocity flow imaging is described. by subtraction of two phase images obtained by varying the flow-encoding gradient, it is possible to extract much larger ranges of velocity components. this differential phase-encoding technique is tested by experiments in a phantom as well as a human volunteer. | 1987 | 3574062 |
| evidence for oh-/h+ permeation across the peritubular cell membrane of rat renal proximal tubule in hco3(-)-free solutions. | membrane potentials and intracellular ph were measured on rat renal proximal tubular cells in vivo to test whether sodium-bicarbonate cotransport across the peritubular cell membrane accepts oh- (or h+ in opposite direction) or whether it requires the co2, hco3-, co3= buffer to operate. it was found that step changes of peritubular ph in nominally hco3(-)-free and co2-free solutions produced qualitatively similar initial potential responses and cell ph responses as changes in peritubular hco3- c ... | 1987 | 3039449 |
| neuroanatomy of memory and amnesia: a case for multiple memory systems. | several different dissociable memory systems are considered in terms of their known or putative sites of pathology. in man, short-term memory can be selectively impaired by left hemisphere lesions near the acoustic projection area. there may be other modality-specific deficits. long-term memory is considered in terms of a) knowledge systems, b) stable associative memory; c) priming, and d) event memory, with special reference to the amnesic syndrome. for each of these categories, clinical neurop ... | 1987 | 3114179 |
| age-dependent activation of glucocorticoid receptors in the cerebral hemispheres of male rats. | the binding of [3h]dexamethasone-receptor complexes to purified nuclei was studied in the cerebral hemispheres of immature (3-week-old) and mature (26-week-old) long-evans male rats to determine the age-related changes, if any, in the physicochemical properties of glucocorticoid receptors. our data show that heat activation (for 45 min at 25 degrees c) significantly enhances the nuclear binding of [3h]dexamethasone-receptor complexes in rats of both ages, with a greater magnitude in immature rat ... | 1987 | 3690338 |
| vaccination against cutaneous leishmaniasis in a murine model. ii. immunologic properties of protective and nonprotective subfractions of soluble promastigote extract. | we have previously demonstrated that balb/c mice can be protected against a fatal infection with leishmania major by i.p. immunization with a soluble leishmanial antigen (sla) preparation in conjunction with the adjuvant, corynebacterium parvum (cp). in this study, sla was separated into nine distinct fractions by anion exchange liquid chromatography, and the fractions were analyzed for their ability to stimulate t cells obtained from immunized mice, to be recognized by vaccine-induced antibodie ... | 1987 | 3499465 |
| evaluation of growth fractions with monoclonal antibodies to human alpha-dna polymerase. | we have established and partially characterized a panel of monoclonal antibodies against alpha-dna polymerase. one of the hybridomas, clone 5f, has been exploited for cell kinetic studies on three colon cancer cell lines, lovo, sw 620, and sw 403, which are endowed with different growth patterns and differentiation status. by an immunoperoxidase method, we could demonstrate the specific intranuclear localization of alpha-dna polymerase during the exponential phase of in vitro growth and contrast ... | 1987 | 3815379 |
| evaluation of growth fractions with monoclonal antibodies to human alpha-dna polymerase. | we have established and partially characterized a panel of monoclonal antibodies against alpha-dna polymerase. one of the hybridomas, clone 5f, has been exploited for cell kinetic studies on three colon cancer cell lines, lovo, sw 620, and sw 403, which are endowed with different growth patterns and differentiation status. by an immunoperoxidase method, we could demonstrate the specific intranuclear localization of alpha-dna polymerase during the exponential phase of in vitro growth and contrast ... | 1987 | 3815379 |
| detection of fish antibody against protein antigen of aeromonas salmonicida by enzyme-linked immunosorbent assay using biotin-avidin system. | antibody against aeromonas salmonicida was detected in sera from immunised or experimentally infected rainbow trout by enzyme-linked immunosorbent assay (elisa) using the biotin-avidin system. the elisa titre correlated well with the agglutinin titres of the sera, but the elisa was found to be more sensitive than the agglutination test. when the rainbow trout serum was separated by column chromatography, antibody activity (determined by elisa and agglutination test) was detected in the igm fract ... | 1987 | 3628989 |
| virulence, cytotoxic and inflammatory activities of vibrio anguillarum and aeromonas salmonicida isolated from cultivated salmonid fish in sweden. | 1987 | 3687646 | |
| construction of an excretion vector and extracellular production of human growth hormone from escherichia coli. | a new excretion vector, peap8, was constructed to develop an excretion system for escherichia coli. this plasmid, derived from peap37, carried the weakly activated kil gene of plasmid pmb9 [kobayashi et al., j. bacteriol. 166 (1986) 728-732] and the penicillinase promoter and signal region of an alkalophilic bacillus sp. to excrete foreign gene products. a gene for human growth hormone (hgh) was joined to this signal sequence through the hindiii site. the recombinant plasmid p8hgh1 thus construc ... | 1987 | 2820841 |
| mechanism of action of somatostatin in the hippocampal slice. | 1987 | 2888131 | |
| cloning of the gene for the surface array protein of aeromonas salmonicida and evidence linking loss of expression with genetic deletion. | a gene bank of dna from the fish pathogenic bacterium aeromonas salmonicida was constructed in the bacteriophage lambda gt11. phage lambda gt11/10g, a recombinant carrying a 4.0-kilobase fragment of a. salmonicida dna, was found to express the surface array protein (a protein) in escherichia coli. sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the protein expressed from the cloned gene had a subunit molecular weight of 49,000, which was identical to that of subunits in the ... | 1987 | 3040676 |
| natural killer cells in viral arthritis. | changes in natural killer (nk) cell activity were studied in patients with polyarthritis associated with rubella or ross river virus infections. in 30 of 32 ross river virus patients, peripheral nk cell activity was depressed at some stage of the disease but returned to normal levels as patients recovered from arthritic symptoms. similar changes did not occur in rubella patients and no difference was found between changes in peripheral nk activity and serum interferon (ifn) levels in rubella pat ... | 1987 | 2443284 |
| role of postnatal gonadal function in the determination of thyrotropin (tsh) releasing hormone-induced tsh response in adult male and female rats. | permanent effects of postnatal gonadal function on the hypothalamo-pituitary-thyroid axis were examined in male and female rats of the wistar-imamichi strain. animals were used at the age of about 10 weeks. neonatally castrated (nc) males showed a significantly higher plasma tsh response to trh (10 micrograms/kg bw, ip) than males castrated at 1, 2, 3, 4, or 6 weeks of age. the tsh response in intact males was similar to that seen in nc males. neonatal androgenization of nc males with 100 microg ... | 1987 | 3032567 |
| fulminant leukemic polyradiculoneuropathy in a case of b-cell prolymphocytic leukemia. a clinicopathologic report. | a 52 year old man with a 10-month history of b-cell prolymphocytic leukemia (pll) died of an apparent acute fulminant polyradiculoneuropathy, a condition generally attributed to paraneoplastic complication. the pathologic examination disclosed diffuse leukemic infiltrations of the peripheral nervous system. it is suggested that this particularly aggressive form of b-cell chronic prolymphocytic leukemia presented a constellation of features that promoted the invasion of the peripheral nervous sys ... | 1987 | 3040217 |
| [involvement of the growth hormone in rapid increases of the potassium concentration of the serum]. | as a rule, the infusion of arginine hydrochloride is followed by an increase in serum potassium. the investigation is concerned with the short-term effect of growth hormone, which can be stimulated by arginine (0.6 g/kg body weight), on potassium concentrations in rats and patients with cerebral death. the administration of human somatotropin causes a significant rise in potassium concentrations in patients with cerebral death (0.4 iu/kg body weight) and in rats (4.0 iu/kg body weight). in compa ... | 1987 | 3439594 |
| differential effects of serotonin (5-ht1a and 5-ht2) agonists and antagonists on renin and corticosterone secretion. | the present study was designed to investigate the effect of distinct serotonin (5-ht1a and 5-ht2) agonists and antagonists on renin and corticosterone secretion. low doses of the selective 5-ht1a agonists 8-hydroxy-2-(di-n-propylamino)tetralin (8-oh-dpat) (5.0-500.0 micrograms/kg, i.p.) and ipsapirone (tvx q 7821; 0.5-2.5 mg/kg, i.p.), and of the 5-hydroxytryptamine (5-ht) agonist mk-212 (2.0 mg/kg, i.p.), did not elevate plasma renin activity (pra) and concentration (prc) 30 min postinjection. ... | 1987 | 2952898 |
| a comparative study of the formation of extracellular proteins by aeromonas salmonicida at two different temperatures. | aeromonas salmonicida was grown in a supplemented 3% (w/v) tryptone soya broth medium at 10 degrees c, a temperature at the lower end of the range over which furunculosis has been observed to occur in the field, and 25 degrees c, the optimum temperature for growth. similar bacterial densities in the range 2.35 +/- 0.05 mg dry wt/ml were achieved in the two cultures at the beginning of the stationary phase of the growth cycle, after 125 h at 10 degrees c and 18 h at 25 degrees c. at this point, a ... | 1987 | 3298187 |
| analysis of structure-activity relationships in renin substrate analogue inhibitory peptides. | on the basis of the minimal octapeptide sequence of the renin substrate, a series of peptides was synthesized containing (3s,4s)-4-amino-3-hydroxy-6-methylheptanoic acid (statine) or (3s,4s)-4-amino-3-hydroxy-5-phenylpentanoic acid (ahppa) at the p1p1' position. some of these peptides also contained nin-formyltryptophan at the p5, p3, or p3' position. renin-inhibitory potency varied over a wide range (from inactive to ic50 = 3 nm). potency was reduced by at least 10-fold when the peptide was sho ... | 1987 | 3302256 |
| effects of polymyxin b nonapeptide on aeromonas salmonicida. | in contrast to polymyxin b-susceptible gram-negative bacteria of human origin, the fish pathogen aeromonas salmonicida was resistant to sensitization by polymyxin b nonapeptide (pmbn) to hydrophobic antibiotics. similarly, sensitization of a. salmonicida strains by pmbn to the bactericidal action of brook trout (salvelinus fontinalis) serum complement was less pronounced than the similar effect of pmbn against other gram-negative bacteria in certain mammalian sera. the surface array protein (a l ... | 1987 | 2823698 |
| differential effects of serotonin (5-ht1a and 5-ht2) agonists and antagonists on renin and corticosterone secretion. | the present study was designed to investigate the effect of distinct serotonin (5-ht1a and 5-ht2) agonists and antagonists on renin and corticosterone secretion. low doses of the selective 5-ht1a agonists 8-hydroxy-2-(di-n-propylamino)tetralin (8-oh-dpat) (5.0-500.0 micrograms/kg, i.p.) and ipsapirone (tvx q 7821; 0.5-2.5 mg/kg, i.p.), and of the 5-hydroxytryptamine (5-ht) agonist mk-212 (2.0 mg/kg, i.p.), did not elevate plasma renin activity (pra) and concentration (prc) 30 min postinjection. ... | 1987 | 2952898 |
| experimental induction of atypical hyperplasia in rat ventral prostate. | 1987 | 2443923 | |
| mechanical stimulation of dorsal root ganglia induces increased production of substance p: a mechanism for pain following nerve root compromise? | the undecapeptide, substance p, is known to be synthesized in cell bodies of dorsal root ganglia. this neuropeptide is also known to modulate sensory, nociceptive transmission postsynaptically on dorsal horn interneurons. in the animal model used in the current study, experimental mechanical stimulation of dorsal root ganglia and nerve roots increased the amounts of substance p, as well as substance p immunoreactivity in cell bodies of the dorsal root ganglia and in the substantia gelatinosa of ... | 1987 | 2443985 |
| inhibition of the aeromonas salmonicida extracellular protease by alpha 2-macroglobulin in the serum of rainbow trout. | one of the major pathogenic factors of aeromonas salmonicida, the bacterium causing furunculosis in fish, is considered to be a protease secreted in the extracellular products. this protease can be inhibited by normal trout and rabbit serum but the factor responsible has not hitherto been identified. the present results demonstrate that the a. salmonicida protease is inhibited by the alpha 2-macroglobulin of rainbow trout serum. this inhibitor accounts for about 9% of the total trypsin inhibitin ... | 1987 | 2461505 |
| [spontaneous rupture of liver tumors with intra-abdominal hemorrhage and hemobillia]. | 1987 | 203744 | |
| [trial with 24-hour home nursing in viborg community: system brings about relief for nursing homes and hospitals]. | 1987 | 260397 | |
| reaction kinetics for positive charge accumulation on the water side of chloroplast photosystem ii. | 1987 | 174952 | |
| experimental laboratory infection of mosquito larvae with fungi of the genus coelomomyces. 1. experiments with coelomomyces psorophorae var. in aedes taeniorhynchus and coelomomyces psorophorae var. in culiseta inornata. | 1986 | 239068 | |
| [wilms' tumor associated with multiple developmental defects]. | 1986 | 180490 | |
| ectopic production of acth by a primary adenocarcinoma of the colon. | 1986 | 214566 | |
| is vasoactive intestinal polypeptide the principal transmitter involved in human penile erection? | previous work from this laboratory reported on the effects of several autacoids and other agents on strips of human corpus cavernosum (cc) muscle. these investigations indicated the presence in the cc muscle of a) atropine-sensitive cholinoceptors, b) alpha- and beta-adrenoceptors and c) a non-adrenergic non-cholinergic mechanism. several recent publications have presented evidence in support of the possibility that vasoactive intestinal polypeptide (vip) is an important, or the chief, transmitt ... | 1986 | 2868132 |
| differential partition of virulent aeromonas salmonicida and attenuated derivatives possessing specific cell surface alterations in polymer aqueous-phase systems. | two-polymer aqueous-phase systems were used to compare via partitioning the surface properties of strains of the fish pathogen aeromonas salmonicida which differed in their ability to produce the surface protein array known as the a layer and in their ability to produce smooth lipopolysaccharide. in these two-phase systems, biological particles are known to partition between the phases in a manner related to a variety of surface properties, including hydrophobicity, charge, and lipid composition ... | 1986 | 2425736 |
| effects of direct immersion in antigen on immunological memory in young carp, cyprinus carpio. | the study asks whether, in fish, antigens encountered early in life can prime the immune system to yield memory responses on subsequent challenge with the same antigen and, if so, whether positive immunity or immunological tolerance is induced. the direct immersion method of vaccination was used to prime 4 week old carp, cyprinus carpio, and was compared with priming by injection. three different forms of antigen were used: the thymus dependent antigen, human gamma globulin (hgg) in soluble and ... | 1986 | 2429432 |
| the ontogeny of humoral immunity in rainbow trout, salmo gairdneri. | the ontogeny of the humoral immune response to a 'thymus dependent' and a 'thymus independent' antigen, human gamma globulin (hgg) and aeromonas salmonicida (as) respectively, was investigated in the fry of rainbow trout, salmo gairdneri, by direct immersion vaccination in the antigens (dose 5 mg/l hgg; 10(8) cells/ml as; 30 minutes) at known ages/weights from 7 days post hatch, and at 1, 2, 3, and 4 months post hatch. half the fry in each group were tested for antibodies 4 weeks after vaccinati ... | 1986 | 2429433 |
| aminergic neurotransmitter and water content changes in rats after transient forebrain ischemia. | we have studied changes of cerebral monoamine metabolism and water content, during recirculation following global transient ischemia (20 min) using the four-vessel occlusion model in rats. levels of monoamines and their metabolites were determined in cortex, striatum, hippocampus, and hypothalamus. water content was evaluated by weight and by the analysis of t1 and t2 relaxation times in 1h-nuclear magnetic resonance. norepinephine levels decreased; 3,4-dihydroxyphenylethylamine, 3,4-dihydroxyph ... | 1986 | 2430056 |
| aminergic neurotransmitter and water content changes in rats after transient forebrain ischemia. | we have studied changes of cerebral monoamine metabolism and water content, during recirculation following global transient ischemia (20 min) using the four-vessel occlusion model in rats. levels of monoamines and their metabolites were determined in cortex, striatum, hippocampus, and hypothalamus. water content was evaluated by weight and by the analysis of t1 and t2 relaxation times in 1h-nuclear magnetic resonance. norepinephine levels decreased; 3,4-dihydroxyphenylethylamine, 3,4-dihydroxyph ... | 1986 | 2430056 |
| a procedure of vestibular decompensation for clinical diagnosis. i. animal experiments. | vestibular lesions are often so effectively compensated that their diagnosis may be difficult. certain chemicals can decompensate the central vestibular system and thus uncover symptoms present before compensation. this study attempts to devise a procedure which can utilize such decompensation for the benefit of clinical diagnosis. unilateral labyrinthectomies were performed in rats. pathological movements and body positions due to the vestibular loss were compensated within about one week. syst ... | 1986 | 3485723 |
| functional domains of the yeast regulatory protein gal4. | in the yeast saccharomyces cerevisiae regulation of the galactose/melibiose regulon rests on a dosage-dependent functional interplay between the positive regulator of transcription, the gal4 protein, and the negative regulator, gal80 protein. we have used this interplay to select in vitro generated fusions between the yeast adh1 promoter and gal4 coding sequence that overproduce gal4 protein, allowing the identification of gal4 protein in crude extracts from yeast. one type of these construction ... | 1986 | 2944111 |
| identification of a conserved domain among cyclic nucleotide phosphodiesterases from diverse species. | partial amino acid sequences have been determined for the ca2+/calmodulin-stimulated cyclic nucleotide phosphodiesterase from bovine brain and the cgmp-stimulated cyclic nucleotide phosphodiesterase from bovine heart. examination of these sequences for homologous segments and comparison with protein sequences derived from the nucleotide sequences of the yeast pde2 gene and the drosophila dunce+ gene [chen, c.-n., denome, s. & davis, r. l. (1986) proc. natl. acad. sci. usa 83, 9313-9317; sass, p. ... | 1986 | 3025833 |
| the characterization of a conjugative r-plasmid isolated from aeromonas salmonicida. | we have analyzed two related r-plasmids isolated from aeromonas salmonicida: par32 and pja8102-1, which encode resistance to chloramphenicol, streptomycin, and sulfonamides (su). the genetic map of pja8102-1 indicated that this plasmid consists of at least three functionally different regions. one is the region conferring ability to transfer (tra), one is the region coding for drug resistance genes (r-det), and one is the region encoding the replication functions (rep). both plasmids contain rep ... | 1986 | 3027727 |
| [imaging of prostatic cancer by 1.5 tesla nuclear resonance tomography]. | twenty-two patients with histologically confirmed carcinomas of the prostate were examined by nuclear magnetic resonance, using a 1.5 tesla magnet (stage t1: one patient, t2: eight patients, t3: six patients, t4: seven patients). in 19 out of the 21 patients in stages t2 to t4, the tumour showed a specific signal intensity. in 12 cases, the tumour signal was more intense than from a normal prostate when using medium repetition and echo delay times; in 19 cases, multi-echo sequences with increasi ... | 1986 | 3008256 |
| complete dissociation between the activation of phosphoinositide turnover and of nadph oxidase by formyl-methionyl-leucyl-phenylalanine in human neutrophils depleted of ca2+ and primed by subthreshold doses of phorbol 12,myristate 13,acetate. | evidences have been provided by many laboratories that the activation of the nadph oxidase in neutrophils by formyl-methionyl-leucyl-phenylalanine (fmlp) is strictly linked to a transduction pathway that involves the stimulation, via gtp binding protein, of the phosphoinositide turnover and the increase in [ca2+]i. the results presented in this paper demonstrate that fmlp can activate the nadph oxidase by triggering a transduction pathway completely independent of phosphoinositide turnover and c ... | 1986 | 3008746 |
| [experimental nephritis induced by coxsackie b4 virus in mice--transient mesangial proliferation associated with acute viremia]. | 1986 | 3018334 | |
| benzodiazepine receptor binding is not altered in human epileptogenic cortical foci. | we measured benzodiazepine binding in cortical tissues from 13 patients with partial seizures. no differences in receptor density (bmax) or affinity (kd) were observed when epileptic foci were compared with nonspiking cortex. | 1986 | 3020475 |
| menadione (2-methyl-1,4-naphthoquinone)-induced ca2+ release from rat-liver mitochondria is caused by nad(p)h oxidation. | incubation of rat-liver mitochondria with menadione in the presence of succinate and rotenone resulted in rapid glutathione and nad(p)h oxidation followed by ca2+ release and mitochondrial swelling. ca2+ release, nad(p)h oxidation and mitochondrial swelling, were also observed in mitochondria from selenium-deficient rats. glutathione was only slowly oxidized, suggesting that glutathione oxidation, and subsequent nad(p)h oxidation via the glutathione peroxidase-glutathione reductase system were n ... | 1986 | 3020812 |
| applicability of coagglutination test for detecting aeromonas salmonicida antigens from extracts of bacterial cultures and experimentally infected tissues of the common carp (cyprinus carpio l.). | 1986 | 2953200 | |
| catecholamine release and potentiation of thromboxane a2 production by nicotine in the greyhound. | thromboxane a2 was generated by infusing arachidonic acid (2.5 micrograms ml-1) into an extra-corporeal circuit of blood withdrawn from anaesthetized dogs, and assayed on a blood-bathed bioassay cascade of porcine and bovine coronary artery strips, chick rectum and rat stomach strip. all tissues except chick rectum were treated with phentolamine and propranolol to abolish direct effects of catecholamines. the arachidonate-induced contractions of artery strips were abolished by a thromboxane synt ... | 1986 | 2937502 |
| electrostatic mechanism of survival of virulent aeromonas salmonicida strains in river water. | the ecological mechanism of survival of aeromonas salmonicida, the bacterial pathogen of fish furunculosis, in river water was investigated by laboratory-based experiments with two virulent strains (which were autoagglutinating) and two virulent strains (which were nonagglutinating). a difference in net electrical charge of a. salmonicida cells was detected by electrophoresis; cells of the virulent strains were negative, whereas cells of the avirulent strains were positive. despite the loss of v ... | 1986 | 2942106 |
| metabolic and morphological alterations of brown adipose tissue after sympathetic denervation in rats. | effects of sympathetic denervation on biochemical and morphological changes of brown adipose tissue (bat) were studied in rats after severing 5 intercostal nerve bundles that enter the unilateral interscapular bat, the contralateral bat being used as the control. four weeks after denervation, there was no appreciable change in bat weight or its dna and protein contents, whereas the triglyceride content was increased significantly. however, the rate of fatty acid synthesis was decreased to about ... | 1986 | 3958437 |
| heme requirement for growth of fastidious atypical strains of aeromonas salmonicida. | the growth of fastidious atypical strains of the fish pathogen aeromonas salmonicida on both solid and liquid media was dependent specifically on a source of heme which was apparently required for initiation of growth at low inoculum densities. thus, hemin enhanced the plating efficiencies of such strains on solid medium and significantly reduced their inoculum-size-dependent lag times in broth. the heme requirement could also be satisfied by hematoporphyrin and, less effectively, by hemoglobin. ... | 1986 | 3963814 |
| the structure of the heptose-3-deoxy-d-mannooctulosonic-acid region in a mutant form of aeromonas salmonicida lipopolysaccharide. | lipopolysaccharide was isolated from a phage-selected mutant of a wild strain of aeromonas salmonicida by the aqueous phenol method. the lipopolysaccharide consisted of the r form, containing per mole, three moles of l-glycero-d-manno-heptopyranose, one mole of 3-deoxy-d-manno-2-octulosonic acid (docla) and lipid a. the docla was not fully assayable by the thiobarbituric acid methods usually used, but its degradation product was detected, after smith degradation of the lipopolysaccharide, either ... | 1986 | 3780744 |
| pain, increasing girth in healthy japanese man. | 1986 | 3021791 | |
| pharmacokinetics of insulin following intravenous and subcutaneous administration in canines. | studies were conducted to examine the absorption and disposition kinetics of insulin in dogs following intravenous (iv) and subcutaneous (sc) administration of commercial preparations. after iv and sc dosing, the plasma levels were described by models which considered basal insulin level contributions. intersubject variation in the disposition kinetics was small with half-lives of 0.52 +/- 0.05 h and total body clearances of 16.21 +/- 2.08 ml min-1 kg-1. calculated insulin plasma secretion rates ... | 1986 | 3535917 |
| diagnosis of plasmodium falciparum infection by spot hybridization assay: specificity, sensitivity, and field applicability. | the spot hybridization assay for the detection of plasmodium falciparum reported here uses as probe a repetitive dna sequence from this species and exhibits a high degree of species specificity. isolates from african, asian, and south american patients were positive in the assay and gametocytes could be detected at the same level of parasitaemia as asexual parasites. an rna probe containing the same repetitive sequence as the dna probe has a detection limit of 1 parasite per 10(6) red blood cell ... | 1986 | 3536140 |
| isotope exchange at equilibrium studies with rat muscle adenylosuccinate synthetase. | the kinetic mechanism of rat muscle adenylosuccinate synthetase was studied by determining the rates of isotope exchange at equilibrium. a random sequential binding mechanism was indicated for both the forward and reverse reactions. aspartate, adenylosuccinate, gdp, and pi were determined to bind in rapid equilibrium. gtp exchanges with both gdp and pi at the same rate, which is similar to the exchange rate of imp with adenylosuccinate. aspartate exchanges with adenylosuccinate at a higher rate ... | 1986 | 3542024 |
| pain, increasing girth in healthy japanese man. | 1986 | 3021791 | |
| ammonia contamination in a milk processing plant--wisconsin. | 1986 | 3084937 | |
| ammonia contamination in a milk processing plant--wisconsin. | 1986 | 3084937 | |
| experimental models of type-i diabetes. | 1986 | 3110756 | |
| milk-feeding maintains the prochymosin production in cells of bovine abomasal mucosa. | the effect of milk-feeding on the development of prochymosin-producing cells in bovine abomasal mucosa was studied using immunochemical methods. as long as the calf was fed milk both mucous neck, and chief cells in the gastric glands, were immunoreactive for prochymosin, the precursor form of a specific milk-clotting enzyme. after weaning, the prochymosin immunoreactivity in chief cells in the lower base of the gastric gland declined and finally disappeared. mucous neck cells and chief cells in ... | 1986 | 3083651 |
| a modified enzyme linked immunosorbent assay (elisa) for monitoring antibody production during experimental aeromonas salmonicida infection in rainbow trout (salmo gairdneri) | 1986 | 3770272 | |
| a comparison of the distribution of extracellular proteins produced by the protease-secreting organism aeromonas salmonicida during aerobic and anaerobic growth. | aeromonas salmonicida was grown aerobically and anaerobically in supplemented 3% (w/v) tryptone soya broth medium for 24 h at 25 degrees c. although the bacterial density achieved was 4.9 times higher in the aerobic culture, the exoprotein produced per unit of bacterial dry weight was only 1.9 times higher than in the anaerobic culture. however, the protease activity of the exoprotein showed a marked reduction anaerobically, being only one-tenth of that of the exoprotein produced aerobically. th ... | 1986 | 3322167 |
| blood changes in carp (cyprinus carpio) induced by ulcerative aeromonas salmonicida infections. | only recently aeromonas salmonicida has been recognized as a significant bacterial pathogen in ulcerative disease of cyprinid fish. our attempts to formulate a vaccine based on bacterial surface antigens were unsuccessful in conferring reliable protection against lethal challenge. this lead us to study pathological changes in the humoral defense system during ulcerative a. salmonicida infection in carp. high numbers of opportunist pathogens such as a. hydrophila and pseudomonas sp. were frequent ... | 1986 | 3765352 |
| does aeromonas salmonicida affect the immune system of carp, cyprinus carpio l.? | aeromonas salmonicida is a significant bacterial pathogen of cyprinid and salmonid fishes causing the systemic disease furunculosis. several observations led us to believe that a. salmonicida was able to evade or suppress the immune system of the fish: injection of whole bacteria or surface antigens was unsuccessful at protecting fish against lethal challenges; memory did not develop in survivors of sublethal infections; diseased fish often carried other opportunistic bacterial pathogens in addi ... | 1986 | 3765353 |
| spontaneous craniopharyngioma in a mouse. | 1986 | 3750744 | |
| identification of neuropeptide y immunoreactivity in the suprachiasmatic nucleus and the lateral geniculate nucleus of some mammals. | the distribution of neuropeptide y (npy) immunoreactivity in the suprachiasmatic nucleus (scn) and the lateral geniculate nucleus (lgn) of 5 kinds of mammals was studied using the peroxidase-anti-peroxidase method. in the scn of the rat, hamster, chipmunk and cat, npy-immunoreactive fibers were detected, particularly in the ventral part. in the 3 rodents and the cat, the somata and fibers of npy-immunoreactive neurons were observed in the lgn. however, immunoreactive structures were not found in ... | 1986 | 3755229 |
| interaction between aeromonas salmonicida and peritoneal macrophages of brook trout (salvelinus fontinalis). | the phagocytic and bactericidal properties of peritoneal macrophages obtained from brook trout (salvelinus fontinalis), injected with either glycogen or a modified freund's complete adjuvant (mfca), were evaluated against an avirulent and a virulent strain of aeromonas salmonicida. avirulent bacteria were effectively phagocytized and killed by macrophages obtained from fish injected with both irritants. with glycogen-elicited macrophages, no enhancement of killing was observed following opsoniza ... | 1986 | 3765344 |
| opiate microinjections into midbrain do not affect the aversiveness of caudal trigeminal stimulation but produce somatotopically organized peripheral hypoalgesia. | high-dose microinjections of morphine sulfate (15 micrograms) and (d-ala2)-met-enkephalin (30 micrograms) were made into the ventral periaqueductal gray of rats. consistent with previous reports using lower doses, both opiates produced hypoalgesia for noxious thermal stimuli applied to the upper and lower body. more hypoalgesia was observed on the face than on the hind legs or tail. current thresholds of aversive reaction to stimulation in the trigeminal subnucleus caudalis were unaffected by mi ... | 1986 | 3768692 |
| microsomal binding sites for antioestrogens in rat liver. properties and detergent solubilization. | the properties of an antioestrogen binding site (aebs), which has high affinity and specificity for nonsteroidal antioestrogens and structurally related compounds, have been studied in rat liver microsomes. when subcellular organelles were separated on percoll density gradients the distribution of the aebs paralleled that of nadph-cytochrome c reductase, indicating that the aebs is associated with the endoplasmic reticulum. saturation analysis showed that [3h]tamoxifen was bound to a single clas ... | 1986 | 3790095 |
| aeromonas salmonicida from salmonids and cyprinids--serological and cultural identification. | 1986 | 3551409 | |
| multiple low-level antibiotic resistance in aeromonas salmonicida. | mutants with multiple low-level antibiotic resistance were isolated from virulent wild-type aeromonas salmonicida strains exposed to a low concentration of any one of several low-molecular-mass (approximately 635 daltons or less) antibiotics. multiple resistance was toward beta-lactam compounds (penicillin g, ampicillin, cloxacillin), quinolones (flumequine, oxolinic acid, nalidixic acid), tetracyclines, chloramphenicol, and novobiocin. susceptibilities of the mutants toward several higher-molec ... | 1986 | 3524440 |
| heterogeneous enzyme immunoassay: detection of anti-candida albicans specific ig-isotypes and mannan antigen. | 1986 | 3534563 | |
| role of sulfate conjugation of catecholamines in blood pressure regulation. | catecholamine sulfates were found to be inactive at vascular receptor sites. only at one nonvascular receptor site important for blood pressure (bp) regulation was dopamine-3-o-sulfate found to be an inhibitory modulator of the adrenocortical secretion of aldosterone in vitro. however, sulfation of catecholamines (ca) does play an important role in bp regulation, as shown by the following observations: sulfoconjugated ca with a long half-life are sometimes better markers of ca release than free ... | 1986 | 3720964 |
| role of sulfate conjugation of catecholamines in blood pressure regulation. | catecholamine sulfates were found to be inactive at vascular receptor sites. only at one nonvascular receptor site important for blood pressure (bp) regulation was dopamine-3-o-sulfate found to be an inhibitory modulator of the adrenocortical secretion of aldosterone in vitro. however, sulfation of catecholamines (ca) does play an important role in bp regulation, as shown by the following observations: sulfoconjugated ca with a long half-life are sometimes better markers of ca release than free ... | 1986 | 3720964 |
| presence of glucocorticoid receptors in cultured thymic epithelial cells. | we investigated the presence of glucocorticoid receptors (gc) in human thymic epithelial cells grown in primary cultures and in a pure epithelial rat cell line. these gr levels were compared to those determined concomitantly in fresh human thymocytes. the average number of sites were 54,457/cell for males (n = 8) and 58,224/cell for females (n = 8) with mean kd values of 1.5 and 1.7 x 10(-8) m, respectively, in cultured human epithelial cells. these results are comparable to those obtained for r ... | 1986 | 3742599 |
| effect of an immunopotentiator on aeromonas salmonicida infection in rainbow trout (salmo gairdneri). | the immunoactive peptide fk-565 (heptanoyl-y-d-glutamyl-(l)-mesodiaminopimelyl-(d)-alanine) was found to induce protection against intraperitoneal aeromonas salmonicida infection in rainbow trout (salmo gairdneri richardson). the survival rate was as high as 60% when fk-565 was given intraperitoneally as a single dose (1mg/kg) one day before bacterial challenge. a non-specific stimulation of phagocytic cells by fk-565 at an early stage of the bacterial infection may contribute to the resistance ... | 1986 | 3765348 |
| two patterns in the aeromonas salmonicida a-layer may reflect a structural transformation that alters permeability. | electron micrographs of negatively stained regular surface layers (a-layers) of aeromonas salmonicida showed two square patterns having p4 symmetry. computer image processing demonstrated that, at a resolution of 2.3 nm, both square arrays were composed of two different morphological units arranged alternatively to give a face-centered lattice in which the four nearest neighbors of each unit were the other type of unit. the lattice constant was slightly but significantly different in the two pat ... | 1986 | 3957865 |
| electrostatic mechanism of survival of virulent aeromonas salmonicida strains in river water. | [this corrects the article on p. 1343 in vol. 51.]. | 1986 | 16347195 |
| significance of extracellular protease for growth of a heterotrophic bacterium, aeromonas salmonicida. | the role of protease produced by a heterotrophic bacterium during growth was investigated with aeromonas salmonicida, the pathogen of fish furunculosis, strain a-7301 and its protease-deficient mutant ntg-1 induced by mutagenesis. strain a-7301 produced extracellular protease in a mixed amino acid medium (composed of gly, ala, val, ile, leu, thr, ser, cys, met, phe, tyr, lys, arg, pro, his, try, asp, asn, glu, and gln at equal concentrations of 0.1 g/liter). its multiplication rate was limited b ... | 1985 | 16346900 |
| porphyrin binding by the surface array virulence protein of aeromonas salmonicida. | congo red binding by virulent a-layer-containing (a+) and avirulent a-layer-deficient (a-) strains of aeromonas salmonicida was examined. congo red binding to a+ cells was enhanced by salt and thus hydrophobically driven, but at low congo red concentrations binding was salt independent. congo red was bound by a+ cells by a kinetically distinct mechanism (kd, 0.25 microm) which was absent in a- isogenic strains. purified a-layer protein ("a protein") protein a also bound congo red with similar af ... | 1985 | 3877720 |
| preparation and properties of fabigg, a chimeric univalent antibody designed to attack tumour cells. | in order to promote the killing of tumour cells by antibody a derivative has been synthesized in which fab'gamma from xenogeneic antibody is thioether-bonded to half-cystine on normal igg of the species to be treated. the resulting entity, fabigg, is obtained with about a 40% yield of the starting fab'gamma. being univalent it evades antigenic modulation. it activates complement efficiently, is minimally immunogenic, and appears to be catabolized at the slow rate characteristic of autologous igg ... | 1985 | 3879458 |
| deficiency in 15-hydroxyprostaglandin dehydrogenase activity after unilateral ureteral obstruction of the dog kidney. | in the present study, metabolism of prostaglandins (pgs) by 15-hydroxyprostaglandin dehydrogenase (15-oh pgdh) was investigated in dog kidneys with ureteral obstruction. after 24 hr of ureteral obstruction, the obstructed kidney and the contralateral kidney were removed and the cytosolic fractions (105,000 x g), enriched in 15-oh pgdh, were prepared from the cortex and medulla. 15-oh pgdh activity was estimated by radiometric assays of the metabolism of [3h]pge2 and [3h]prostacyclin. cortical 15 ... | 1985 | 3881582 |
| loss of virulence in a protease-deficient mutant of aeromonas salmonicida. | the importance of extracellular protease production by aeromonas salmonicida, the bacterial pathogen of fish furunculosis, was investigated with four virulent strains (which were autoagglutinative, hemagglutinative, resistant to fish serum, adhesive to fish tissue culture, protease positive, hemolysin positive, and leukocytolysin positive) and three avirulent strains (which were nonagglutinative, nonhemagglutinative, sensitive to serum, nonadhesive, protease positive, hemolysin positive, and leu ... | 1985 | 3884507 |
| [probability in the evaluation of the reliability of bactericidal effects]. | 1985 | 4029632 | |
| immunity to aeromonas salmonicida in coho salmon (oncorhynchus kisutch) induced by modified freund's complete adjuvant: its non-specific nature and the probable role of macrophages in the phenomenon. | juvenile coho salmon (oncorhynchus kisutch), vaccinated with one intraperitoneal injection of formalin-killed virulent aeromonas salmonicida cells suspended in saline, showed increased protection against approximately one ld60 of homologous challenge administered at 30 days post-vaccination. under similar conditions, coho vaccinated with a modified complete freund's adjuvant (mfca) alone were also equally protected. when measured against a more severe a. salmonicida challenge of approximately on ... | 1985 | 4043481 |
| the purification and some properties of h-lysin from aeromonas salmonicida. | h-lysin from aeromonas salmonicida has been purified 1770-fold by freeze fractionation, ammonium sulphate precipitation, ion exchange chromatography and gel filtration chromatography. the purified material was predominantly h-lysin, devoid of detectable t-lysin, caseinase or gelatinase activity, although glycerophospholipid: cholesterol acyltransferase (gcat) activity was present. the results suggested that h-lysin and gcat activities were due to different extracellular products. studies of the ... | 1985 | 4045422 |
| growth response of mouse lymphoma cells to low concentrations of mercuric chloride. | three growth rate experiments involving several sampling points were performed to investigate the previous finding that very low concentrations of hgcl2 inhibit the growth of murine lymphoblasts in vitro. however, results presented here do not confirm this, there being no significant differences between the three independent growth rate experiments. | 1985 | 3832669 |
| [effect of titanium phosphate, zirconium oxide and a mixture of ammonium phosphotungstenate with zirconium phosphate on the elimination of radioactive cesium from the bodies of rats]. | 1985 | 3835674 |