Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| major surface protein complex of treponema denticola induces the production of tumor necrosis factor alpha, interleukin-1 beta, interleukin-6 and matrix metalloproteinase 9 by primary human peripheral blood monocytes. | treponema denticola is a micro-organism that is involved in the pathogenesis of periodontitis. major surface protein complex (mspc), which is expressed on the envelope of this treponeme, plays a key role in the interaction between t. denticola and gingival cells. the peptidoglycan extracted from t. denticola induces the production of a large variety of inflammatory mediators by macrophage-like cells, suggesting that individual components of t. denticola cells induce the inflammatory response dur ... | 2010 | 20337896 |
| the analysis of oral microbial communities of wild-type and toll-like receptor 2-deficient mice using a 454 gs flx titanium pyrosequencer. | although mice have long served as an animal model for periodontitis, information on the composition of their indigenous oral microbiota is limited. the aim of the current study was to characterize mouse oral bacterial flora by applying extensive parallel pyrosequencing using the latest model pyrosequencer, a roche/454 genome sequencer flx titanium. in addition, the effect of toll-like receptor (tlr) 2 deficiency on oral microbiota was evaluated. | 2010 | 20370919 |
| use of luminescent leptospira interrogans for enumeration in biological assays. | rapid and reliable in vitro methods for the detection of pathogenic leptospires, such as leptospira interrogans, are lacking. the present study investigated the use of luminescence to replace the existing enumeration techniques. transposon tnsc189 was modified to incorporate the luxcdabe cassette from photorhabdus luminescens and was used to construct luminescent leptospira spp. there was a linear relationship between luminescence and cell number, with the theoretical detection limit being less ... | 2010 | 20375235 |
| mixture of periodontopathogenic bacteria influences interaction with kb cells. | the purpose of this study was to investigate the adhesion and invasion of periodontopathogenic bacteria in varied mixed infections and the release of interleukins from an epithelial cell line (kb cells). | 2010 | 20380884 |
| a highly selective oligopeptide binding protein from the archaeon sulfolobus solfataricus. | sso1273 of sulfolobus solfataricus was identified as a cell surface-bound protein by a proteomics approach. sequence inspection of the genome revealed that the open reading frame of sso1273 is associated in an operon-like structure with genes encoding all the remaining components of a canonical protein-dependent atp-binding cassette (abc) transporter. sso1273 gene expression and sso1273 protein accumulation on the cell surface were demonstrated to be strongly induced by the addition of a peptide ... | 2010 | 20382765 |
| fam5c contributes to aggressive periodontitis. | aggressive periodontitis is characterized by a rapid and severe periodontal destruction in young systemically healthy subjects. a greater prevalence is reported in africans and african descendent groups than in caucasians and hispanics. we first fine mapped the interval 1q24.2 to 1q31.3 suggested as containing an aggressive periodontitis locus. three hundred and eighty-nine subjects from 55 pedigrees were studied. saliva samples were collected from all subjects, and dna was extracted. twenty-one ... | 2010 | 20383335 |
| cleavage of iggs by proteases associated with invasive diseases: an evasion tactic against host immunity? | the effective functioning of immunoglobulins and igg mabs in removing pathological cells requires that the antigen binding regions and the fc (effector) domain act in concert. the hinge region that connects these domains itself presents motifs that engage fc receptors on immune effector cells to achieve cell lysis. in addition, sequences in the lower hinge/ch2 and further down the ch2 region are involved in c1q binding and complement-mediated cell killing. proteolytic enzymes of little relevance ... | 2010 | 20400859 |
| response of subgingival bacteria to smoking cessation. | it has been demonstrated that smoking cessation alters the subgingival microbial profile; however, the response of individual bacteria within this ecosystem has not been well studied. the aim of this investigation, therefore, was to longitudinally examine the effect of smoking cessation on the prevalence and levels of selected subgingival bacteria using molecular approaches for bacterial identification and enumeration. subgingival plaque was collected from 22 smokers at the baseline and 12 month ... | 2010 | 20410352 |
| periodontal disease-associated micro-organisms in peri-menopausal and post-menopausal women using or not using hormone replacement therapy. a two-year follow-up study. | despite conflicting results on the use of hormone replacement therapy (hrt) there is no doubt that many women benefit from it. women using hrt are known to be more health conscious in general with putative positive implications in the mouth. however, we observed recently in our cohort hardly any difference in oral health status between hrt-users and non-users. there are only a few studies about hrt and oral microbiota. we hypothesized that counts of periodontal micro-organisms are lower in healt ... | 2010 | 20429938 |
| treponema denticola prcb is required for expression and activity of the prca-prtp (dentilisin) complex. | the treponema denticola surface protease complex, consisting of prtp protease (dentilisin) and two auxiliary polypeptides (prca1 and prca2), is believed to contribute to periodontal disease by degrading extracellular matrix components and disrupting host intercellular signaling. previously, we showed that transcription of the protease operon initiates upstream of tde0760 (herein designated prcb), the open reading frame immediately 5' of prca-prtp. the prcb gene is conserved in t. denticola strai ... | 2010 | 20435733 |
| secreted aspergillus fumigatus protease alp1 degrades human complement proteins c3, c4, and c5. | the opportunistic human pathogenic fungus aspergillus fumigatus is a major cause of fungal infections in immunocompromised patients. innate immunity plays an important role in the defense against infections. the complement system represents an essential part of the innate immune system. this cascade system is activated on the surface of a. fumigatus conidia and hyphae and enhances phagocytosis of conidia. a. fumigatus conidia but not hyphae bind to their surface host complement regulators factor ... | 2010 | 20498262 |
| peptidylarginine deiminase from porphyromonas gingivalis citrullinates human fibrinogen and α-enolase: implications for autoimmunity in rheumatoid arthritis. | to investigate protein citrullination by the periodontal pathogen porphyromonas gingivalis as a potential mechanism for breaking tolerance to citrullinated proteins in rheumatoid arthritis (ra). | 2010 | 20506214 |
| the recolonization hypothesis in a full-mouth or multiple-session treatment protocol: a blinded, randomized clinical trial. | to test recolonization of periodontal lesions after full-mouth scaling and root planing (fm-srp) or multiple session-srp (ms-srp) in a randomized clinical trial and whether fm-srp and ms-srp result in different clinical outcomes. | 2010 | 20507375 |
| structural and enzymatic insights into the atp binding and autophosphorylation mechanism of a sensor histidine kinase. | desk is a sensor histidine kinase (hk) that allows bacillus subtilis to respond to cold shock, triggering the adaptation of membrane fluidity via transcriptional control of a fatty acid desaturase. it belongs to the hk family hpk7, which includes the nitrogen metabolism regulators narx/q and the antibiotic sensor lias among other important sensor kinases. structural information on different hk families is still scarce and several questions remain, particularly concerning the molecular features t ... | 2010 | 20507988 |
| peptidoglycan crosslinking relaxation promotes helicobacter pylori's helical shape and stomach colonization. | the mechanisms by which bacterial cells generate helical cell shape and its functional role are poorly understood. helical shape of the human pathogen helicobacter pylori may facilitate penetration of the thick gastric mucus where it replicates. we identified four genes required for helical shape: three lytm peptidoglycan endopeptidase homologs (csd1-3) and a ccma homolog. surrounding the cytoplasmic membrane of most bacteria, the peptidoglycan (murein) sacculus is a meshwork of glycan strands j ... | 2010 | 20510929 |
| periodontitis: a polymicrobial disruption of host homeostasis. | periodontitis, or gum disease, affects millions of people each year. although it is associated with a defined microbial composition found on the surface of the tooth and tooth root, the contribution of bacteria to disease progression is poorly understood. commensal bacteria probably induce a protective response that prevents the host from developing disease. however, several bacterial species found in plaque (the 'red-complex' bacteria: porphyromonas gingivalis, tannerella forsythia and treponem ... | 2010 | 20514045 |
| bacterial and human peptidylarginine deiminases: targets for inhibiting the autoimmune response in rheumatoid arthritis? | peptidylarginine deiminases (pads) convert arginine within a peptide (peptidylarginine) into peptidylcitrulline. citrullination by human pads is important in normal physiology and inflammation. porphyromonas gingivalis, a major pathogen in periodontitis, is the only prokaryote described to possess pad. p. gingivalis infection may generate citrullinated peptides, which trigger anti-citrullinated peptide antibodies. in susceptible individuals, host protein citrullination by human pads in the joint ... | 2010 | 20553633 |
| in vitro identification of novel plasminogen-binding receptors of the pathogen leptospira interrogans. | leptospirosis is a multisystem disease caused by pathogenic strains of the genus leptospira. we have reported that leptospira are able to bind plasminogen (plg), to generate active plasmin in the presence of activator, and to degrade purified extracellular matrix fibronectin. | 2010 | 20582320 |
| porphyromonas gingivalis and treponema denticola mixed microbial infection in a rat model of periodontal disease. | porphyromonas gingivalis and treponema denticola are periodontal pathogens that express virulence factors associated with the pathogenesis of periodontitis. in this paper we tested the hypothesis that p. gingivalis and t. denticola are synergistic in terms of virulence; using a model of mixed microbial infection in rats. groups of rats were orally infected with either p. gingivalis or t. denticola or mixed microbial infections for 7 and 12 weeks. p. gingivalis genomic dna was detected more frequ ... | 2010 | 20592756 |
| regt can modulate gingipain activity and response to oxidative stress in porphyromonas gingivalis. | recombinant vima protein can interact with the gingipains and several other proteins that may play a role in its biogenesis in porphyromonas gingivalis. in silico analysis of pg2096, a hypothetical protein that was shown to interact with vima, suggests that it may have environmental stress resistance properties. to further evaluate the role(s) of pg2096, the predicted open reading frame was pcr amplified from p. gingivalis w83 and insertionally inactivated using the ermf-ermam antibiotic-resista ... | 2010 | 20595264 |
| complement and periodontitis. | although the complement system is centrally involved in host defense, its overactivation or deregulation (e.g., due to inherent host genetic defects or due to pathogen subversion) may excessively amplify inflammation and contribute to immunopathology. periodontitis is an oral infection-driven chronic inflammatory disease which exerts a systemic impact on health. this paper reviews evidence linking complement to periodontal inflammation and pathogenesis. clinical and histological observations sho ... | 2010 | 20599785 |
| the hpk2-rrp2 two-component regulatory system of treponema denticola: a potential regulator of environmental and adaptive responses. | treponema denticola levels in the gingival crevice become elevated as periodontal disease develops. oral treponemes may account for as much as 40% of the total bacterial population in the periodontal pocket. the stimuli that trigger enhanced growth of t. denticola, and the mechanisms associated with the transmission of these signals, remain to be defined. we hypothesize that the t. denticola open reading frames tde1970 (histidine kinase) and tde1969 (response regulator) constitute a functional t ... | 2010 | 20618698 |
| molecular characterization of treponema denticola infection-induced bone and soft tissue transcriptional profiles. | treponema denticola is associated with subgingival biofilms in adult periodontitis and with acute necrotizing ulcerative gingivitis. however, the molecular mechanisms by which t. denticola impacts periodontal inflammation and alveolar bone resorption remain unclear. here, we examined changes in the host transcriptional profiles during a t. denticola infection using a murine calvarial model of inflammation and bone resorption. t. denticola was injected into the subcutaneous soft tissue over the c ... | 2010 | 20618700 |
| differential host response to lps variants in amniochorion and the tlr4/md-2 system in macaca nemestrina. | microbial-specific factors are likely critical in determining whether bacteria trigger preterm labor. structural variations in lipopolysaccharide (lps), a component of gram-negative bacteria, can determine whether lps has an inflammatory (agonist) or anti-inflammatory (antagonist) effect through toll-like receptor 4 (tlr4). our objective was to determine whether amniochorion could discriminate between lps variants in a nonhuman primate model. we also cloned macaca nemestrina tlr4 and md-2 and co ... | 2010 | 20619890 |
| dentipain, a streptococcus pyogenes ides protease homolog, is a novel virulence factor of treponema denticola. | treponema denticola is a major pathogen of chronic periodontitis. analysis of the t. denticola genome revealed a gene orthologous with a cysteine protease-encoding gene from streptococcus pyogenes (ides). ides interferes with igg-dependent opsonophagocytosis by specific cleavage of igg molecules. analysis of this gene (termed idet) revealed it to encode a two-domain protein whose n-terminus is composed of tandem immunoglobulin-like domains followed by a c-terminal ides-like protease domain. in t ... | 2010 | 20635859 |
| "gum bug, leave my heart alone!"--epidemiologic and mechanistic evidence linking periodontal infections and atherosclerosis. | evidence from epidemiologic studies suggests that periodontal infections are independently associated with subclinical and clinical atherosclerotic vascular disease. although the strength of the reported associations is modest, the consistency of the data across diverse populations and a variety of exposure and outcome variables suggests that the findings are not spurious or attributable only to the effects of confounders. analysis of limited data from interventional studies suggests that period ... | 2010 | 20639510 |
| espp, a serine protease of enterohemorrhagic escherichia coli, impairs complement activation by cleaving complement factors c3/c3b and c5. | hemolytic-uremic syndrome (hus) is a life-threatening disorder characterized by hemolytic anemia, thrombocytopenia, and renal insufficiency. it is caused mainly by infections with enterohemorrhagic escherichia coli (ehec). recently, shiga toxin 2, the best-studied virulence factor of ehec, was reported to interact with complement, implying that complement may be involved in the pathogenesis of ehec-induced hus. the aim of the present study was to investigate whether or not the serine protease es ... | 2010 | 20643852 |
| examination of periodontal pathogens in stenotic valve specimens and in whole blood samples in patients affected by aortic valve stenosis and chronic periodontitis. | periodontitis may be a risk factor for atherosclerosis and coronary heart disease. the influence of periodontal pathogens in cardiovascular diseases needs further investigation. therefore, the aims of this clinical study are: to test the presence of periodontal bacteria dna in aortic valves and to assess the concomitant presence of the same periodontal bacteria dna in whole blood samples in patients affected by aortic valve stenosis and chronic periodontitis. nineteen consecutive patients (12 ma ... | 2010 | 20646351 |
| 16s rrna-based detection of oral pathogens in coronary atherosclerotic plaque. | atherosclerosis develops as a response of the vessel wall to injury. chronic bacterial infections have been associated with an increased risk for atherosclerosis and coronary artery disease. the ability of oral pathogens to colonize in coronary atheromatous plaque is well known. | 2010 | 20657096 |
| characterization of the normal bacterial flora in peri-implant sulci of partially and completely edentulous patients. | to characterize the normal bacterial flora and evaluate the presence of periodontopathogenic bacteria around dental implants and to correlate them with the periodontal flora or, in completely edentulous patients, the alveolar gingival flora. | 2010 | 20657863 |
| the ability of the bana test to detect different levels of p. gingivalis, t. denticola and t. forsythia. | the aim of this study was to evaluate the ability of the bana test to detect different levels of porphyromonas gingivalis, treponema denticola and tannerella forsythia or their combinations in subgingival samples at the initial diagnosis and after periodontal therapy. periodontal sites with probing depths between 5-7 mm and clinical attachment level between 5-10 mm, from 53 subjects with chronic periodontitis, were sampled in four periods: initial diagnosis (t0), immediately (t1), 45 (t2) and 60 ... | 2010 | 20658043 |
| [association between phenytoin-induced gingival hyperplasia and periodontopathic bacteria in institutionalized patients with severe motor and intellectual disabilities]. | the association between phenytoin-induced gingival hyperplasia (pigh) and five major periodontopathic bacteria was investigated by real-time pcr in 12 subjects (30.6 +/- 7.95 years of age) with severe motor and intellectual disabilities. the subjects had not received antibiotics or periodontal therapy within three months. a healthy gingival site or a site with the lowest gingival hyperplasia score (minimum site), and a site with the maximum score of gingival hyperplasia (maximum site) on each su ... | 2010 | 20662307 |
| td92, an outer membrane protein of treponema denticola, induces osteoclastogenesis via prostaglandin e(2)-mediated rankl/osteoprotegerin regulation. | periodontitis is a chronic inflammatory disease of the periodontium that causes significant alveolar bone loss. osteoclasts are bone-resorbing multinucleated cells. osteoblasts regulate osteoclast differentiation by expression of rankl and osteoprotegerin (opg). td92 is a surface-exposed outer membrane protein of treponema denticola, a periodontopathogen. although it has been demonstrated that td92 acts as a stimulator of various proinflammatory mediators, the role of td92 in alveolar bone resor ... | 2010 | 20682013 |
| relationship between oral status and prevalence of periodontopathic bacteria on the tongues of elderly individuals. | colonization of periodontopathic bacteria is associated with increased risk of systemic diseases. however, few studies have investigated the relationships between oral status factors and health-related quality of life (hr-qol) and the prevalence of such bacteria in elderly individuals. this study investigated the prevalence of porphyromonas gingivalis, prevotella intermedia, treponema denticola and tannerella forsythia in 165 community-dwelling functionally independent 85-year-old japanese indiv ... | 2010 | 20688951 |
| a simplified erythromycin resistance cassette for treponema denticola mutagenesis. | the primary selectable marker for the genetic studies of treponema denticola is a hybrid gene cassette containing both ermf and ermam (ermb) genes. ermb functions in escherichia coli, while ermf has been assumed to confer resistance in t. denticola. we demonstrate here that ermb is sufficient for erythromycin selection in t. denticola and that the native ermb promoter drives ermb expression. | 2010 | 20691222 |
| dichotomy of gingipains action as virulence factors: from cleaving substrates with the precision of a surgeon's knife to a meat chopper-like brutal degradation of proteins. | 2010 | 20712631 | |
| contribution of porphyromonas gingivalis lipopolysaccharide to periodontitis. | 2010 | 20712633 | |
| virulence mechanisms of tannerella forsythia. | 2010 | 20712636 | |
| virulence factors of treponema denticola. | 2010 | 20712637 | |
| periodontal innate immune mechanisms relevant to atherosclerosis and obesity. | 2010 | 20712641 | |
| antigenic activity of bacterial endodontic contents from primary root canal infection with periapical lesions against macrophage in the release of interleukin-1beta and tumor necrosis factor alpha. | periradicular tissue chronic stimulation by endotoxin may cause apical periodontitis. this study investigated the microbial profile and the levels of endotoxin found in primary root canal infection with apical periodontitis, determined their antigenicity against macrophages through the levels of interleukin (il)-1beta and tumor necrosis factor alpha (tnf-alpha), and evaluated their relationship with clinical and radiographic findings. | 2010 | 20728711 |
| selective substitution of amino acids limits proteolytic cleavage and improves the bioactivity of an anti-biofilm peptide that targets the periodontal pathogen, porphyromonas gingivalis. | the interaction of the periodontal pathogen, porphyromonas gingivalis, with oral streptococci such as streptococcus gordonii precedes colonization of the subgingival pocket and represents a target for limiting p. gingivalis colonization of the oral cavity. previous studies showed that a synthetic peptide (designated bar) derived from the antigen i/ii protein of s. gordonii was a potent competitive inhibitor of p. gingivalis adherence to s. gordonii and subsequent biofilm formation. here we show ... | 2010 | 20800634 |
| effect of khat chewing on periodontal pathogens in subgingival biofilm from chronic periodontitis patients. | existing in vitro and in vivo data suggest that khat may have a favorable effect on periodontal microbiota. the purpose of this study was to assess the effect of khat chewing on major periodontal pathogens in subgingival plaque samples from subjects with chronic periodontitis. | 2010 | 20816745 |
| functional characterization of the incomplete phosphotransferase system (pts) of the intracellular pathogen brucella melitensis. | in many bacteria, the phosphotransferase system (pts) is a key player in the regulation of the assimilation of alternative carbon sources notably through catabolic repression. the intracellular pathogens brucella spp. possess four pts proteins (eintr, npr, eiiantr and an eiia of the mannose family) but no pts permease suggesting that this pts might serve only regulatory functions. | 2010 | 20844759 |
| cellular architecture of treponema pallidum: novel flagellum, periplasmic cone, and cell envelope as revealed by cryo electron tomography. | high-resolution cryo electron tomography (cryo-et) was utilized to visualize treponema pallidum, the causative agent of syphilis, at the molecular level. three-dimensional (3d) reconstructions from 304 infectious organisms revealed unprecedented cellular structures of this unusual member of the spirochetal family. high-resolution cryo-et reconstructions provided detailed structures of the cell envelope, which is significantly different from that of gram-negative bacteria. the 4-nm lipid bilayer ... | 2010 | 20850455 |
| clonal analysis of the microbiota of severe early childhood caries. | severe early childhood caries is a microbial infection that severely compromises the dentition of young children. the aim of this study was to characterize the microbiota of severe early childhood caries. | 2010 | 20861633 |
| surface immunolabeling and consensus computational framework to identify candidate rare outer membrane proteins of treponema pallidum. | treponema pallidum reacts poorly with the antibodies present in rabbit and human syphilitic sera, a property attributed to the paucity of proteins in its outer membrane. to better understand the basis for the syphilis spirochete's "stealth pathogenicity," we used a dual-label, 3-step amplified assay in which treponemes encapsulated in gel microdroplets were probed with syphilitic sera in parallel with anti-flaa antibodies. a small (approximately 5 to 10%) but reproducible fraction of intact trep ... | 2010 | 20876295 |
| investigation of subgingival profile of periodontopathic bacteria using polymerase chain reaction. | periodontopathic bacteria such as porphyromonas gingivalis, aggregatibacter actinomycetemcomitans, tannerella forsythia, campylobacter rectus and treponema denticola play an important role in the initiation and progression of periodontitis. the aim of this investigation was to evaluate the relationship between periodontal clinical parameters and the subgingival profile of periodontopathic bacteria. twenty-six periodontitis patients (23-62 years of age; mean age, 40.2±13.2) with no systemic disea ... | 2010 | 20877160 |
| tannerella forsythia infection-induced calvarial bone and soft tissue transcriptional profiles. | tannerella forsythia is associated with subgingival biofilms in adult periodontitis, although the molecular mechanisms contributing to chronic inflammation and loss of periodontal bone remain unclear. we examined changes in the host transcriptional profiles during a t. forsythia infection using a murine calvarial model of inflammation and bone resorption. tannerella forsythia was injected into the subcutaneous soft tissue over calvariae of balb/c mice for 3 days, after which the soft tissues and ... | 2010 | 20883221 |
| using dgge profiling to develop a novel culture medium suitable for oral microbial communities. | more than 700 bacterial species have been detected in the human oral cavity. they form highly organized microbial communities and are responsible for many oral infectious diseases, such as dental caries and periodontal disease. the prevention and treatment of these diseases require a comprehensive knowledge of oral microbial communities, which largely relies on culture-dependent methods to provide detailed phenotypic and physiological analysis of these communities. however, most of the currently ... | 2010 | 20883224 |
| an update on periodontic-orthodontic interrelationships. | talking about periodontic-orthodontic interrelationships is related primarily to the 1960s, where a generalized increase in salivary bacterial counts, especially lactobacillus, had been shown after orthodontic band placement. the purpose of this article is to provide the dental practitioner with basic understanding of the interrelationship between periodontics and orthodontics by means of representing classical studies, and, to give an update on this topic by demonstrating the most recent opinio ... | 2010 | 20922083 |
| potential of the tannerella forsythia s-layer to delay the immune response. | the periodontal pathogen tannerella forsythia possesses a glycosylated s-layer as an outermost cell decoration. while the s-layer provides a selection advantage to the bacterium in the natural habitat, its virulence potential remains to be investigated. in the present study, the immune responses of human macrophages and gingival fibroblasts upon stimulation with wild-type t. forsythia and an s-layer-deficient mutant were investigated. the mrna expression levels of the pro-inflammatory mediators ... | 2010 | 20929722 |
| potential of the tannerella forsythia s-layer to delay the immune response. | the periodontal pathogen tannerella forsythia possesses a glycosylated s-layer as an outermost cell decoration. while the s-layer provides a selection advantage to the bacterium in the natural habitat, its virulence potential remains to be investigated. in the present study, the immune responses of human macrophages and gingival fibroblasts upon stimulation with wild-type t. forsythia and an s-layer-deficient mutant were investigated. the mrna expression levels of the pro-inflammatory mediators ... | 2010 | 20929722 |
| virulence factors of the oral spirochete treponema denticola. | there is compelling evidence that treponemes are involved in the etiology of several chronic diseases, including chronic periodontitis as well as other forms of periodontal disease. there are interesting parallels with other chronic diseases caused by treponemes that may indicate similar virulence characteristics. chronic periodontitis is a polymicrobial disease, and recent animal studies indicate that co-infection of treponema denticola with other periodontal pathogens can enhance alveolar bone ... | 2010 | 20940357 |
| real-time polymerase chain reaction of "red complex" (porphyromonas gingivalis, tannerella forsythia, and treponema denticola) in periradicular abscesses. | the red complex bacteria (porphyromonas gingivalis, tannerella forsythia, and treponema denticola) have been implicated, either singly or in combination, in the development of various forms of periodontal diseases. the aim of this study was to investigate the presence of "red complex" in acute periradicular abscesses by real-time polymerase chain reaction (pcr) method. | 2010 | 20955954 |
| hemagglutinin/adhesin domains of porphyromonas gingivalis play key roles in coaggregation with treponema denticola. | porphyromonas gingivalis and treponema denticola are major pathogens of periodontal disease. coaggregation between microorganisms plays a key role in the colonization of the gingival crevice and the organization of periodontopathic biofilms. we investigated the involvement of surface ligands of p. gingivalis in coaggregation. two triple mutants of p. gingivalis lacking arg-gingipain a (rgpa), lys-gingipain (kgp) and hemagglutinin a (haga) or rgpa, arg-gingipain b (rgpb) and kgp showed significan ... | 2010 | 21039921 |
| characterization of a novel family of fibronectin-binding proteins with m23 peptidase domains from treponema denticola. | interactions with fibronectin are important in the virulence strategies of a range of disease-related bacteria. the periodontitis-associated oral spirochaete treponema denticola expresses at least two fibronectin-binding proteins, designated msp (major surface protein) and oppa (oligopeptide-binding protein homologue). to identify other t. denticola outer membrane fibronectin-binding proteins, the amino acid sequence of the treponema pallidum fibronectin-binding protein tp0155 was used to survey ... | 2010 | 21040511 |
| transcriptional profiles of treponema denticola in response to environmental conditions. | the periodontal pathogen t. denticola resides in a stressful environment rife with challenges, the human oral cavity. knowledge of the stress response capabilities of this invasive spirochete is currently very limited. whole genome expression profiles in response to different suspected stresses including heat shock, osmotic downshift, oxygen and blood exposure were examined. most of the genes predicted to encode conserved heat shock proteins (hsps) were found to be induced under heat and oxygen ... | 2010 | 21048920 |
| early bacterial colonization and soft tissue health around zirconia and titanium abutments: an in vivo study in man. | aim: to compare the early bacterial colonization and soft tissue health of mucosa adjacent to zirconia (zro(2) ) and titanium (ti) abutment surfaces in vivo. materials and methods: twenty edentulous subjects received two endosseous mandibular implants. the implants were fitted with either a zro(2) or a ti abutment (non-submerged implant placement, within-subject comparison, left-right randomization). sulcular bacterial sampling and the assessment of probing pocket depth, recession and bleeding o ... | 2010 | 21054554 |
| characterization of novel bovine gastrointestinal tract treponema isolates and comparison with bovine digital dermatitis treponemes. | this study aimed to isolate and characterize treponemes present in the bovine gastrointestinal (gi) tract and compare them with bovine digital dermatitis (bdd) treponemes. seven spirochete isolates were obtained from the bovine gi tract, which, on the basis of 16s rrna gene comparisons, clustered within the genus treponema as four novel phylotypes. one phylotype was isolated from several different gi tract regions, including the omasum, colon, rumen, and rectum. these four phylotypes could be di ... | 2010 | 21057019 |
| characterization of novel bovine gastrointestinal tract treponema isolates and comparison with bovine digital dermatitis treponemes. | this study aimed to isolate and characterize treponemes present in the bovine gastrointestinal (gi) tract and compare them with bovine digital dermatitis (bdd) treponemes. seven spirochete isolates were obtained from the bovine gi tract, which, on the basis of 16s rrna gene comparisons, clustered within the genus treponema as four novel phylotypes. one phylotype was isolated from several different gi tract regions, including the omasum, colon, rumen, and rectum. these four phylotypes could be di ... | 2010 | 21057019 |
| targeting selenium metabolism and selenoproteins: novel avenues for drug discovery. | selenoproteins play a wide range of roles in metabolism and oxidative stress defense and are produced by organisms in all three domains of life. recent evidence has been presented that metal based cancer drugs target the selenol nucleophile of the active site selenocysteine in thioredoxin reductase isoenzymes. other metals and metalloids, such as tin, arsenic and gold, have also recently been shown to form stable complexes with hydrogen selenide, a required precursor for the synthesis of selenop ... | 2010 | 21069141 |
| systemic inflammatory responses in progressing periodontitis during pregnancy in a baboon model. | this study tested the hypothesis that pregnant female baboons exhibit increased levels of various inflammatory mediators in serum resulting from ligature-induced periodontitis, and that these profiles would relate to periodontal disease severity/extent in the animals. the animals were sampled at baseline (b), mid-pregnancy (mp; two quadrants ligated) and at delivery (d; four quadrants ligated). all baboons developed increased plaque, gingival inflammation and bleeding, pocket depths and attachme ... | 2010 | 21070210 |
| polybacterial challenge enhances hiv reactivation in latently infected macrophages and dendritic cells. | a polymicrobial infection comprising subgingival biofilms is the trigger for the chronic immunoinflammatory lesions of periodontitis. these microbial biofilms interface with host immune cells that increase with progressing disease and could result in hiv reactivation in hiv-1-infected patients. previous reports have focused on the ability of monospecies challenge of macrophages and dendritic cells to detail molecular aspects of their detection and signalling pathways. this study provides a semin ... | 2010 | 21073452 |
| microbiota of deciduous endodontic infections analysed by mda and checkerboard dna-dna hybridization. | to evaluate the microbiota of endodontic infections in deciduous teeth by checkerboard dna-dna hybridization after uniform amplification of dna in samples by multiple displacement amplification (mda). | 2010 | 21083570 |
| microbiota of deciduous endodontic infections analysed by mda and checkerboard dna-dna hybridization. | to evaluate the microbiota of endodontic infections in deciduous teeth by checkerboard dna-dna hybridization after uniform amplification of dna in samples by multiple displacement amplification (mda). | 2010 | 21083570 |
| the effect of a single episode of antimicrobial photodynamic therapy in the treatment of experimental periodontitis. microbiological profile and cytokine pattern in the dog mandible. | the purpose of this study was to evaluate the effect of a single application of antimicrobial photodynamic therapy (apdt) on microbiological profile and cytokine pattern in dogs. periodontal disease was induced by placing 3.0 silk ligatures around the mandibular pre-molars bilaterally during 8 weeks. the dogs were randomly treated with apdt using a dye/laser system, scaling and root planning (srp), or with the association of treatments (srp + apdt). plaque samples were collected at baseline, 1, ... | 2010 | 21086009 |
| integrating '-omics' and natural product discovery platforms to investigate metabolic exchange in microbiomes. | the microbiome is an abundance of microorganisms within a host (e.g. human microbiome). these microorganisms produce small molecules and metabolites that have been shown to affect and dictate the physiology of an individual. functional knowledge of these molecules, often produced for communication or defense, will reveal the interplay between microbes and host in health and disease. the vast diversity in structure and function of microbiome-associated small molecules necessitate tools that will ... | 2010 | 21087892 |
| integrating '-omics' and natural product discovery platforms to investigate metabolic exchange in microbiomes. | the microbiome is an abundance of microorganisms within a host (e.g. human microbiome). these microorganisms produce small molecules and metabolites that have been shown to affect and dictate the physiology of an individual. functional knowledge of these molecules, often produced for communication or defense, will reveal the interplay between microbes and host in health and disease. the vast diversity in structure and function of microbiome-associated small molecules necessitate tools that will ... | 2010 | 21087892 |
| one-stage full-mouth disinfection as a therapeutic approach for generalized aggressive periodontitis. | background: data concerning treatment outcomes in patients with generalized aggressive periodontitis (gagp) are limited. the aim of this study is to investigate 6-month clinical and microbiologic outcomes of the one-stage full-mouth disinfection (osfmd) in the management of patients with gagp. methods: twenty-seven patients with advanced gagp were included in this prospective follow-up intervention study. clinical and microbiologic parameters were collected at baseline and 3 and 6 months after t ... | 2010 | 21091345 |
| periodontal pathogens interfere with quorum-sensing-dependent virulence properties in streptococcus mutans. | the mechanism by which periodontal pathogens dominate at disease sites is not yet understood. one possibility is that these late colonizers antagonize the quorum-sensing systems of early colonizers and render those early colonizers less resistant to environmental factors. in this study, we utilized streptococcus mutans, a well-documented oral streptococcus with many quorum-sensing-dependent properties, as an example of an earlier colonizer antagonized by periodontal pathogens. | 2010 | 21108642 |
| the treponema denticola chymotrypsin-like protease dentilisin induces matrix metalloproteinase-2-dependent fibronectin fragmentation in periodontal ligament cells. | periodontal disease is a bacterially mediated chronic inflammatory disease that results in destruction of the periodontal ligament (pdl) and alveolar bone that surround and support the dentition. while their precise roles are not well understood, periodontal pathogens, including treponema denticola, are believed to initiate the destructive inflammatory responses and dysregulation of tissue homeostasis that characterize the disease. these responses are believed to result from both proinflammatory ... | 2010 | 21115719 |
| antialarmin effect of tick saliva during the transmission of lyme disease. | tick saliva has potent immunomodulatory properties. in arthropod-borne diseases, this effect is largely used by microorganisms to increase their pathogenicity and to evade host immune responses. we show that in lyme borreliosis, tick salivary gland extract and a tick saliva protein, salp15, inhibit in vitro keratinocyte inflammation induced by borrelia burgdorferi sensu stricto or by the major outer surface lipoprotein of borrelia, ospc. chemokines (interleukin-8 [il-8] and monocyte chemoattract ... | 2010 | 21134970 |
| antialarmin effect of tick saliva during the transmission of lyme disease. | tick saliva has potent immunomodulatory properties. in arthropod-borne diseases, this effect is largely used by microorganisms to increase their pathogenicity and to evade host immune responses. we show that in lyme borreliosis, tick salivary gland extract and a tick saliva protein, salp15, inhibit in vitro keratinocyte inflammation induced by borrelia burgdorferi sensu stricto or by the major outer surface lipoprotein of borrelia, ospc. chemokines (interleukin-8 [il-8] and monocyte chemoattract ... | 2010 | 21134970 |
| the central region of the msp gene of treponema denticola has sequence heterogeneity among clinical samples, obtained from patients with periodontitis. | treponema denticola is an oral spirochete involved in the pathogenesis and progression of periodontal disease. of its virulence factors, the major surface protein (msp) plays a role in the interaction between the treponeme and host. to understand the possible evolution of this protein, we analyzed the sequence of the msp gene in 17 t. denticola positive clinical samples. | 2010 | 21138575 |
| community signalling between streptococcus gordonii and porphyromonas gingivalis is controlled by the transcriptional regulator cdhr. | interspecies signalling between porphyromonas gingivalis and streptococcus gordonii serves to constrain development of dual species communities. contact with s. gordonii propagates a tyrosine phosphorylation-dependent signal within p. gingivalis that culminates in reduced transcription of adhesin and signalling genes. here we demonstrate the involvement of the p. gingivalis orphan luxr family transcription factor pgn_1373, which we designate cdhr, in this control pathway. expression of cdhr is e ... | 2010 | 21143321 |
| role of the cysteine protease interpain a of prevotella intermedia in breakdown and release of haem from haemoglobin. | the gram-negative oral anaerobe prevotella intermedia forms an iron(iii) protoporphyrin ix pigment from haemoglobin. the bacterium expresses a 90 kda cysteine protease, inpa (interpain a), a homologue of streptococcus pyogenes streptopain (speb). the role of inpa in haemoglobin breakdown and haem release was investigated. at ph 7.5, inpa mediated oxidation of oxyhaemoglobin to hydroxymethaemoglobin [in which the haem iron is oxidized to the fe(iii) state and which carries oh- as the sixth co-ord ... | 2009 | 19814715 |
| cryo-electron tomography elucidates the molecular architecture of treponema pallidum, the syphilis spirochete. | cryo-electron tomography (cet) was used to examine the native cellular organization of treponema pallidum, the syphilis spirochete. t. pallidum cells appeared to form flat waves, did not contain an outer coat and, except for bulges over the basal bodies and widening in the vicinity of flagellar filaments, displayed a uniform periplasmic space. although the outer membrane (om) generally was smooth in contour, om extrusions and blebs frequently were observed, highlighting the structure's fluidity ... | 2009 | 19820083 |
| progression of chronic periodontitis can be predicted by the levels of porphyromonas gingivalis and treponema denticola in subgingival plaque. | chronic periodontitis is an inflammatory disease of the supporting tissues of the teeth associated with bacteria. diagnosis is achieved retrospectively by clinical observation of attachment loss. predicting disease progression would allow for targeted preventive therapy. the aim of this study was to monitor disease progression in patients on a maintenance program and determine the levels of specific bacteria in subgingival plaque samples and then examine the ability of the clinical parameters of ... | 2009 | 19832799 |
| differential virulence and innate immune interactions of type i and ii fimbrial genotypes of porphyromonas gingivalis. | the fima-encoded fimbriae of the periodontal pathogen porphyromonas gingivalis display genetic diversity. type i fimbriated p. gingivalis (pg-i) has been most widely studied at the molecular level, whereas pg-ii is the most frequent isolate from severe periodontitis. | 2009 | 19832800 |
| subgingival bacterial colonization profiles correlate with gingival tissue gene expression. | periodontitis is a chronic inflammatory disease caused by the microbiota of the periodontal pocket. we investigated the association between subgingival bacterial profiles and gene expression patterns in gingival tissues of patients with periodontitis. a total of 120 patients undergoing periodontal surgery contributed with a minimum of two interproximal gingival papillae (range 2-4) from a maxillary posterior region. prior to tissue harvesting, subgingival plaque samples were collected from the m ... | 2009 | 19835625 |
| autoaggregation response of fusobacterium nucleatum. | fusobacterium nucleatum is a gram-negative oral bacterial species associated with periodontal disease progression. this species is perhaps best known for its ability to adhere to a vast array of other bacteria and eukaryotic cells. numerous studies of f. nucleatum have examined various coaggregation partners and inhibitors, but it is largely unknown whether these interactions induce a particular genetic response. we tested coaggregation between f. nucleatum atcc strain 25586 and various species ... | 2009 | 19837836 |
| negative correlation of distributions of streptococcus cristatus and porphyromonas gingivalis in subgingival plaque. | porphyromonas gingivalis is one of the major causative agents of adult periodontitis. one of the features of this periodontal pathogen is its ability to attach to a variety of oral bacterial surfaces and to colonize subgingival dental plaque. we have shown that streptococcus cristatus cc5a inhibits expression of fima, a gene encoding the major protein subunit of long fimbriae in p. gingivalis; as a result, s. cristatus interrupts formation of p. gingivalis biofilms. here we further demonstrate t ... | 2009 | 19846640 |
| oral bacteria induce a differential activation of human immunodeficiency virus-1 promoter in t cells, macrophages and dendritic cells. | the human immunodeficiency virus (hiv) can integrate into t cells, macrophages and dendritic cells resulting in a latent infection. reports have also demonstrated that various microbial and host cell factors can trigger hiv reactivation leading to hiv recrudescence, potentially undermining highly active antiretroviral therapies. | 2009 | 19702954 |
| crystallization and preliminary x-ray analysis of betac-s lyases from two oral streptococci. | hydrogen sulfide, which causes oral malodour, is generally produced from l-cysteine by the action of betac-s lyase from oral bacteria. the betac-s lyases from two oral bacteria, streptococcus anginosus and s. gordonii, have been cloned, overproduced, purified and crystallized. x-ray diffraction data were collected from the two types of crystals using synchrotron radiation. the crystal of s. anginosus betac-s lyase belonged to the orthorhombic space group p2(1)2(1)2(1), with unit-cell parameters ... | 2009 | 19724121 |
| elafin is specifically inactivated by rgpb from porphyromonas gingivalis by distinct proteolytic cleavage. | abstract porphyromonas gingivalis, the major causative bacterium of periodontitis, contributes significantly to elevated proteolytic activity at periodontal pockets owing to the presence of both bacteria and host, predominantly neutrophil-derived, serine proteases. normally the activity of the latter enzymes is tightly regulated by endogenous proteins, including elafin, a potent neutrophil elastase and proteinase 3 inhibitor released from epithelial cells at sites of inflammation. here, we repor ... | 2009 | 19747076 |
| an unusual autopsy case of pyogenic liver abscess caused by periodontal bacteria. | pyogenic liver abscess (pla) formation is thought to originate from the transmission of infection via three major routes including the biliary tract, portal vein and hepatic artery. however, about 50% of pla cases are considered to be cryptogenic. here we report an unusual autopsy case of pla associated with periodontopathic bacterial infection. a 59-year-old female suddenly developed cardiopulmonary arrest and died. despite macroscopic and microscopic examinations, the infectious routes and sou ... | 2009 | 19762989 |
| distribution of periodontopathic bacterial species in japanese children with developmental disabilities. | recent developments in molecular biological techniques have enabled rapid detection of periodontopathic bacterial species in clinical specimens. accumulated evidence suggests that detection of specific bacterial species enables identification of subjects at high risk for the onset of periodontitis. we investigated the distribution of 10 selected periodontopathic bacterial species in dental plaque specimens obtained from children with disabilities who were attending daycare centers. | 2009 | 19772671 |
| characterization and structure of the manganese-responsive transcriptional regulator scar. | the streptococcal coaggregation regulator (scar) of streptococcus gordonii is a manganese-dependent transcriptional regulator. when intracellular manganese concentrations become elevated, scar represses transcription of the scacba operon, which encodes a manganese uptake transporter. a member of the dtxr/mntr family of metalloregulators, scar shares sequence similarity with other family members, and many metal-binding residues are conserved. here, we show that scar is an active dimer, with two d ... | 2009 | 19795834 |
| salivary interleukin-1beta concentration and the presence of multiple pathogens in periodontitis. | this study aimed to find salivary enzymes and/or cytokines that would reflect periodontitis, alone or in combination with salivary microbial markers. | 2009 | 19799718 |
| saliva enables the antimicrobial activity of ll-37 in the presence of proteases of porphyromonas gingivalis. | proteolysis is a common microbial virulence mechanism that enables the destruction of host tissue and evasion from host defense mechanisms. antimicrobial peptides, also known as host defense peptides, are effector molecules of the innate immunity that demonstrate a broad range of antimicrobial and immunoregulatory activities. deficiency of the human ll-37 antimicrobial peptide was previously correlated with severe periodontal disease. porphyromonas gingivalis, the major pathogen associated with ... | 2009 | 19805540 |
| characterizing traditionally defined periodontal disease in hiv+ adults. | results have varied from previous studies examining the level and extent of periodontal disease (pd) in hiv-1 infected (hiv+) adults. these studies used different methodologies to measure and define pd and examined cohorts with divergent characteristics. inconsistent methodological approaches may have resulted in the underestimation of traditionally-defined pd in hiv+ individuals. | 2009 | 19624697 |
| microbiological profile of untreated subjects with localized aggressive periodontitis. | the microbial profile of localized aggressive periodontitis (lagp) has not yet been determined. therefore, the aim of this study was to evaluate the subgingival microbial composition of lagp. | 2009 | 19637996 |
| microbiological risk for periodontitis of abutment teeth in patients with removable partial dentures. | tannerella forsythia, porphyromonas gingivalis and treponema denticola are identified as the red complex which exists in high proportions in periodontally diseased patients. the aim of this study was to assess the microbiological risk for periodontitis of abutment teeth in subjects wearing removable partial dentures (rpds) by measuring the red complex using bana-zymetm test. thirty-eight subjects (mean age: 62.2 years) with kennedy class ii partially edentulous arch participated in this study. t ... | 2009 | 19650858 |
| strong and persistent microbial and inflammatory stimuli overcome the genetic predisposition to higher matrix metalloproteinase-1 (mmp-1) expression: a mechanistic explanation for the lack of association of mmp1-1607 single-nucleotide polymorphism genotypes with mmp-1 expression in chronic periodontitis lesions. | our objective was to evaluate the association between the mmp1-1607 single-nucleotide polymorphism (snp), periodontopathogens and inflammatory cytokines with matrix metalloproteinase-1 (mmp-1) mrna levels in vitro and in vivo. | 2009 | 19659894 |
| global proteome analysis of leptospira interrogans. | comparative global proteome analyses were performed on leptospira interrogans serovar copenhageni grown under conventional in vitro conditions and those mimicking in vivo conditions (iron limitation and serum presence). proteomic analyses were conducted using itraq and lc-esi-tandem mass spectrometry complemented with two-dimensional gel electrophoresis and maldi-tof mass spectrometry. a total of 563 proteins were identified in this study. altered expression of 65 proteins, including upregulatio ... | 2009 | 19663501 |
| efflux-mediated drug resistance in bacteria: an update. | drug efflux pumps play a key role in drug resistance and also serve other functions in bacteria. there has been a growing list of multidrug and drug-specific efflux pumps characterized from bacteria of human, animal, plant and environmental origins. these pumps are mostly encoded on the chromosome, although they can also be plasmid-encoded. a previous article in this journal provided a comprehensive review regarding efflux-mediated drug resistance in bacteria. in the past 5 years, significant pr ... | 2009 | 19678712 |
| association between periodontitis and anti-cardiolipin antibodies in buerger disease. | anti-cardiolipin (cl) antibodies can be induced in buerger disease (bd), an inflammatory occlusive disorder affecting peripheral blood vessels, in response to bacteria bearing homology to the tlrvyk peptide of a phospholipid-binding plasma protein beta-2-glycoprotein i. tlrvyk homologies are present in porphyromonas gingivalis (tlriyt) and treponema denticola (tlalyk). this study investigated the association between periodontal infection and anti-cl antibodies in bd patients. | 2009 | 19678860 |
| differences in subgingival microflora of korean and german periodontal patients. | the goal of this study was to characterize the microbiological profile in samples of subgingival plaque taken from periodontal patients with different ethnic origin. | 2009 | 19058781 |
| hica of escherichia coli defines a novel family of translation-independent mrna interferases in bacteria and archaea. | toxin-antitoxin (ta) loci are common in free-living bacteria and archaea. ta loci encode a stable toxin that is neutralized by a metabolically unstable antitoxin. the antitoxin can be either a protein or an antisense rna. so far, six different ta gene families, in which the antitoxins are proteins, have been identified. recently, makarova et al. (k. s. makarova, n. v. grishin, and e. v. koonin, bioinformatics 22:2581-2584, 2006) suggested that the hicab loci constitute a novel ta gene family. us ... | 2009 | 19060138 |