Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| antibacterial mechanism of volatile fatty acids in the intestinal tract of pigs against escherichia coli. | 1986 | 3529751 | |
| experimental escherichia coli endotoxin-induced sensitisation and abortion in sows. | subcutaneous injection of 235 micrograms endotoxin from e coli 08: k87, k88ab into two sows 14 days before the expected farrowing date elicited a toxic shock reaction but the sows recovered and farrowed normally. no reaction was observed in two other sows which had received endotoxin-free 0.9 per cent saline. in a subsequent pregnancy, the same four sows were injected subcutaneously with 23 mg of the same endotoxin preparation. those which had previously received endotoxin exhibited a severe sho ... | 1986 | 3532245 |
| mortality in swine herds endemically infected with haemophilus pleuropneumoniae: effect of immunization with cross-reacting lipopolysaccharide core antigens of escherichia coli. | the benefit of increased immunity to cross-reacting lipopolysaccharide core antigens of gram-negative bacteria induced by vaccination with an rc mutant of escherichia coli 0111:b4 (strain j5) was evaluated in commercial swine herds endemically infected with haemophilus pleuropneumoniae. weanling pigs were vaccinated im with e coli j5 (group 1) before the expected time of h pleuropneumoniae infection. clinical signs, antibiotic treatment frequency, mortality, growth performance (days to market we ... | 1986 | 3532872 |
| [assessment of the effectiveness of vaccines against coli dysentery of piglets]. | 1986 | 3532990 | |
| evaluation of selective media for primary isolation of treponema hyodysenteriae and treponema innocens. | a total of 2450 samples of feces, intestinal contents and colon mucosal scrapings were bacteriologically examined. a total of 53 strains of treponema sp. were isolated, and 45 strains of bacteroides sp., 30 strains of e. coli, 30 strains of micrococcus sp. and 10 strains of streptococcus d isolates were randomly selected. growth promoting studies showed statistically significant stimulation of treponema sp. growth by yeast extract, chicken egg yolk and rumen fluid. different growth inhibitors we ... | 1986 | 3533408 |
| endotoxemia in yucatan miniature pigs: metabolic derangements and experimental therapies. | endotoxemia is a frequent complication of many health disorders. it is characterized by systemic release of a variety of endogenous inflammatory mediators which effect cardiovascular depression, reductions in organ blood flow, tissue ischemia and derangements in cellular metabolism leading to death. during a continuous intravenous infusion of escherichia coli lipopolysaccharide, the chronology of alterations in hepatosplanchnic blood flow, hepatic carbohydrate metabolism and pancreatic insulin s ... | 1986 | 3534441 |
| acute inflammatory effects of intratracheally instilled escherichia coli endotoxin and sonicated suspension of haemophilus pleuropneumoniae in swine. | a single bolus of either escherichia coli endotoxin, sonicated suspension of haemophilus pleuropneumoniae, or pyrogen-free normal saline was intratracheally instilled in six week old specific-pathogen-free pigs. pigs exposed to e. coli endotoxin developed fever, leukopenia followed by leukocytosis, and endotoxemia. leukocytosis was the only clinical abnormality noted in pigs receiving the sonicated suspension of h. pleuropneumoniae. at one day postexposure, focal areas of atelectasis and consoli ... | 1986 | 3539296 |
| apramycin and gentamicin resistance in escherichia coli and salmonellas isolated from farm animals. | since the aminoglycoside antibiotic apramycin was licensed for veterinary use in 1980, all isolates of escherichia coli and salmonellas received at the central veterinary laboratory have been monitored for resistance to apramycin and the related antibiotic gentamicin. during the period 1982-4, the incidence of resistance in e. coli to apramycin increased from 0.6% in 1982 to 2.6% in 1984. in salmonellas the incidence of resistance to apramycin increased from 0.1% in 1982 to 1.4% in 1984. resista ... | 1986 | 3540112 |
| [effect of intestinal microflora on the pathogenesis of cholera intoxication]. | germ-free minipigs, previously treated with bacteroids, develop cholinergic reaction after the intragastric administration of vibrio cholerae exotoxin. the intensity of this reaction, disturbances in homeostasis, and the character of morphological changes depend on the dose of choleragen, the bacteroid strain, and the presence of the concomitant (escherichia coli) and residual microflora in the intestine. | 1986 | 3541468 |
| structural and functional relationships between fumarase and aspartase. nucleotide sequences of the fumarase (fumc) and aspartase (aspa) genes of escherichia coli k12. | the nucleotide sequences of two segments of dna (2250 and 2921 base-pairs) containing the functionally related fumarase (fumc) and aspartase (aspa) genes of escherichia coli k12 were determined. the fumc structural gene comprises 1398 base-pairs (466 codons, excluding the initiation codon), and it encodes a polypeptide of mr 50353 that resembles the fumarases of bacillus subtilis 168 (citg-gene product), rat liver and pig heart. the fumc gene starts 140 base-pairs downstream of the structurally- ... | 1986 | 3541901 |
| flow of resistance genes in the environment and from animals to man. | some antibiotic-resistant bacteria occur naturally in the environment but many arise by contamination with human and animal excreta in sewage, slurry and manure. these may be ingested as contaminants of water, animal feeds and cultivated foods or, in the case of animals, by licking their environment. gene transfer has been demonstrated in voided calf faeces and the resultant transconjugants, subsequently ingested, colonized the calf gut. identified strains of antibiotic-resistant escherichia col ... | 1986 | 3542937 |
| antiprotease targeting: altered specificity of alpha 1-antitrypsin by amino acid replacement at the reactive centre. | alpha-1 antitrypsin (alpha 1at) is an efficient inhibitor of the human neutrophil proteases, elastase and cathepsin g. the reactive centre p1 residue (met358) of alpha 1at is important in defining the specificity of inhibition; furthermore, oxidation of this residue results in a loss of inhibitor activity. there is evidence that oxidative inactivation of alpha 1at may be involved in the pathogenesis of pulmonary emphysema associated with cigarette smoking. we have studied the effect of a series ... | 1986 | 3544150 |
| another animal escherichia coli cytopathic factor. | 1986 | 3544462 | |
| lung mechanics with relation to pulmonary haemodynamics, gas exchange and extravascular lung water in mechanically ventilated endotoxaemic pigs. | in a porcine model employing a continuous i.v. infusion of e. coli endotoxin the pathophysiology of early adult respiratory distress syndrome was studied with main emphasis on the early changes in lung mechanics and their relation to changes in pulmonary haemodynamics, gas exchange and extravascular lung water in intermittent positive pressure ventilated (ippv) pigs under ketamine anaesthesia. six animals served as controls and revealed no major physiological changes. nine animals received endot ... | 1986 | 3544623 |
| photobiological activity of certain pyranocoumarin derivatives: potential agents for the photochemotherapy of psoriasis. | the photobiological properties of a series of pyranocoumarin derivatives having linear (xanthyletines) and angular structure (seselins) have been studied; while the linear derivative carrying the methyl geminal group, typical of the parent natural compound, appeared to be entirely inactive in all tests performed, very probably because of the steric hindrance in the dark interaction with dna, 4 substances lacking in this group showed a marked capacity for inhibiting dna synthesis in ehrlich cells ... | 1986 | 3547353 |
| [phospholipase a from the pancreas and e. coli]. | 1986 | 3547505 | |
| experimental porcine eperythrozoonosis: t-lymphocyte suppression and misdirected immune responses. | immune responses and hematologic alterations were investigated in splenectomized pigs after im inoculation with eperythrozoon suis. early hematologic alterations were massive parasitism of rbc, severe hypoglycemia, moderate bilirubinemia, and mild anemia; later findings included severe anemia, minimal parasitism of rbc, spontaneous agglutination of rbc at 25 c and 4 c which was reversible at 37 c, transient thrombocytopenia, and mild bilirubinemia. the humoral immune responses consisting of a tr ... | 1985 | 3874572 |
| reaction of substrates with 35s-thiophosphorylated succinyl-coa synthetase of pig heart. similarities to the case of the escherichia coli enzyme. | guanosine 5'-o-(3-thio)triphosphate (gtp gamma s) was found to be a substrate of pig heart succinyl-coa synthetase with km and kcat values of 3 microm and 0.23 s-1, respectively. the corresponding values with gtp as substrate were 48 microm and 65 s-1. 35s-thiophosphorylated enzyme was prepared by incubation of pig heart succinyl-coa synthetase with [35s]gtp gamma s. a comparison was made of thiophosphoryl group release by substrates from this alpha beta (one active site) enzyme with that of the ... | 1985 | 3882686 |
| channeling of a beta-oxidation intermediate on the large subunit of the fatty acid oxidation complex from escherichia coli. | the kinetic properties of the fatty acid oxidation complex from escherichia coli were studied with the aim of elucidating the functional consequence of having enoyl-coa hydratase and 3-hydroxyacyl-coa dehydrogenase associated with a multifunctional polypeptide. the kinetic parameters of individual enzymes were determined and used in model calculations based on a published theory (storer, a. c., and cornish-bowden, a. (1974) biochem. j. 141, 205-209) to predict the kinetic behavior of a system of ... | 1985 | 3882701 |
| resistance of gnotobiotic large white and chinese piglets to in vivo attachment of a k88ab enterotoxigenic escherichia coli strain. | in vivo adhesion of a k88ab-positive enterotoxigenic escherichia coli (etec) strain to the small intestinal wall of gnotobiotic, colostrum-deprived chinese and large white piglets was investigated. a non-enterotoxigenic, attachment factor-deprived e. coli strain, inoculated in association with the k88ab etec strain, was used as a marker to determine the content residues on the intestinal walls of gnotobiotic piglets. both strains were selectively numerated in the luminal contents and on the wash ... | 1985 | 3883453 |
| naturally occurring plasmid coding for heat-labile enterotoxin production and drug resistance from escherichia coli strain of porcine origin. | 1985 | 3884865 | |
| evaluation of procholeragenoid against experimental colibacillosis in piglets of vaccinated dams. | a toxoid prepared from the toxin of vibrio cholera was adjuvanted with aluminium hydroxide and used for immunisation of pregnant gilts. litters of these and of non-vaccinates were experimentally challenged with escherichia coli producing either heat labile and heat stable (lt and st) enterotoxins or st enterotoxin only. both the challenge strains of e coli produced high rates of mortality (64 and 68 per cent) and morbidity (80 and 100 per cent) in litters of non-vaccinated dams. statistically hi ... | 1985 | 3885554 |
| haemagglutinins & enterotoxins in escherichia coli strains from human urinary tract infections. | 1985 | 3886540 | |
| linkage of genes for heat-stable enterotoxin, drug resistance, k99 antigen, and colicin in bovine and porcine strains of enterotoxigenic escherichia coli. | linkages among genes for production of escherichia coli heat-stable enterotoxin (st), drug resistance, k99 antigen, and colicin were investigated in 2 bovine and 3 porcine strains of enterotoxigenic e coli. in conjugation experiments, all 5 isolates transferred enterotoxigenicity and the ability to produce k99; 4 of the 5 isolates also transferred antibiotic resistance markers, and 3 colicinogenic strains transmitted the ability to produce colicin. in 2 of the 3 colicin-producing strains, the ge ... | 1985 | 3888010 |
| effect of intravenous and intraduodenal administration of escherichia coli endotoxin on the porcine pancreas as evaluated by changes in the serum cationic trypsin-like immunoreactivity. | in six pigs intravenous administration of escherichia coli endotoxin caused septic shock and a significant increase in serum cationic trypsin-like immunoreactivity (ctli), with activation of cationic trypsinogen to trypsin and formation of complexes between cationic trypsin, on the one hand, and alpha 2-macroglobulin and alpha 1-antitrypsin, on the other, compatible with acute pancreatitis. in contrast, intraduodenal infusion of e. coli endotoxin to seven other pigs was without effect on the gen ... | 1985 | 3890137 |
| the effects of oxytetracycline on the intestinal escherichia coli flora of newly weaned pigs. | four recently weaned pigs were dosed orally with oxytetracycline. this caused a rapid increase in the incidence of tetracycline resistance (tcr) among escherichia coli isolates from the faecal flora. the isolates were differentiated further on the basis of o-serogroup, biotype and resistance pattern. there was no evidence that the administration of the antibiotic selected for a few tcr clones, but rather a relatively large number of tcr strains were identified during the dosing period. using sel ... | 1985 | 3894511 |
| in vivo escherichia coli k88 adhesion in gnotobiotic piglets: comparison between large white and chinese piglets. | 1985 | 3895235 | |
| effects of escherichia coli heat-stable enterotoxin, atropine, clonidine, and morphine on chloride efflux from isolated enterocytes. | the effects of escherichia coli heat-stable enterotoxin (st) on chloride efflux rate were investigated in 3 fractions of enterocytes isolated in a villus-to-crypt gradient from porcine jejunum. there was no difference in chloride efflux rates between mature and immature cells from controls. heat-stable enterotoxin significantly increased chloride efflux in all fractions. morphine inhibited st-augmented secretion in mature enterocytes. atropine or clonidine had no effect. calcium efflux rates and ... | 1985 | 3904545 |
| acquisition of genes from an o18:k1:h7 colv+ strain of escherichia coli renders intracranially-inoculated e. coli k12 highly virulent for chickens, ducks and guinea-pigs but not mice. | the virulence of intracranially-inoculated mutant forms of an o18ac:k1:h7 colv+ strain of escherichia coli (designated mw) that lacked different combinations of its o and k antigens and colv, and of an e. coli k12 strain to which these characters had been transmitted was studied in mice, chickens, ducks and guinea-pigs. the o18+k1+colv+ form of mw was highly virulent for chickens and mice but the corresponding form of k12 was only highly virulent for chickens; the o18-k1-colv- forms of both stra ... | 1985 | 3905956 |
| a porcine model of early adult respiratory distress syndrome induced by endotoxaemia. | to study the pathophysiology of early adult respiratory distress syndrome (ards) induced by sepsis, spontaneously breathing pigs under ketamine anaesthesia were investigated. twenty animals were infused i.v. with e. coli endotoxin (10 micrograms . h-1 . kg-1) over 6 h, and ten control animals received physiological saline. in the controls, cardiac output (qt) and o2 delivery decreased slightly. there were no changes in pulmonary gas exchange, pulmonary haemodynamics or extravascular lung water ( ... | 1985 | 3909709 |
| in vivo aspects of urogastrone-epidermal growth factor. | recent evidence indicates that human urogastrone-epidermal growth factor originates in submandibular and brunner's glands and that serum levels are low (less than 1 ng ml-1). it occurs in many secreted fluids to a much greater extent and many tissues are thus exposed to concentrations greater than 100 ng ml-1. rapid actions in vivo of uro-egf include the ability to inhibit gastric acid secretion at low doses (250 ng kg h-1 in humans) and to provide cytoprotective effects against ulcerogenic agen ... | 1985 | 3914989 |
| the study of fecal-escherichia coli peritonitis-induced septic shock in a neonatal pig model. | peritonitis-induced septic shock in the neonate is associated with a high mortality. because there exists no clinically relevant model to study resuscitation of these patients, a model using the neonatal pig was developed. after arterial and central venous cannulation, and placement of a left pulmonary-artery thermodilution catheter, 12 anesthetized neonatal pigs were "resuscitated" with fluids (5% albumin in lactated ringer's solution at 15 ml/kg/hr), antibiotics, and correction of acidemia. th ... | 1985 | 3915235 |
| the role of intramembrane ca2+ in the hydrolysis of the phospholipids of escherichia coli by ca2+-dependent phospholipases. | ca2+-dependent phospholipases a require ca2+ concentrations in the millimolar range for optimal activity toward artificial substrates. because ca2+-dependent phospholipases a2 degrade the phospholipids of escherichia coli, treated with the membrane-active antibiotic polymixin b equally well with and without added ca2+ (weiss, j., beckerdite-quagliata, s., and elsbach, p. (1979) j. biol. chem. 254, 11010-11014), we have examined the possibility that intramembrane ca2+ can provide the ca2+ needed ... | 1985 | 3918043 |
| analysis of receptor-binding site in escherichia coli enterotoxin. | heat-labile enterotoxin produced by enterotoxigenic escherichia coli and cholera enterotoxin are both composed of a and b subunits. the a subunit is an enzymatically active adp-ribosylating subunit, while the b subunit, consisting of 103 amino acids, binds the toxin to a receptor, gm1-ganglioside, on the cell surface. a mutant isolated after treatment of e. coli producing heat-labile enterotoxin with n-methyl-n'-nitro-n-nitrosoguanidine produces a b subunit that is unable to bind to ganglioside. ... | 1985 | 3924916 |
| insulin-related material in microbes: similarities and differences from mammalian insulins. | we have reported that extracts of escherichia coli and tetrahymena grown in synthetic media contained material that reacted specifically in the immunoassay and bioassay for insulin. one additional strain of tetrahymena and four of e. coli yielded amounts of material similar to those reported previously. in addition to their behavior on sephadex g-50, the immunoactive insulin-related materials from the microbial sources behaved like authentic vertebrate insulins in their ability to be adsorbed to ... | 1985 | 3933801 |
| immunopharmacological activities of a synthetic counterpart of a biosynthetic lipid a precursor molecule and of its analogs. | synthetic lipid a analogs (compounds 404 through 406) were examined for their immunopharmacological activities. these compounds had two amide-bound and two ester-bound (r)-3-hydroxytetradecanoyl groups at the c-2 and c-2' and the c-3 and c-3' positions, respectively, of beta (1-3)glucosamine disaccharide. in all of the in vitro assays, these synthetic compounds exhibited high activities comparable to those of a reference lipid a prepared from escherichia coli o8:k27 re-mutant strain f515. the co ... | 1985 | 3980084 |
| effect of lidamidine-hcl on escherichia coli heat-stable enterotoxin-induced jejunal water and electrolyte secretion in neonatal piglets. | neonatal piglets were anesthetized, and two jejunal loops, 20 cm in length, were prepared. then, either water or 0.12, 0.25, 0.5 or 1.0 mg/kg of lidamidine-hcl was injected intraduodenally on a randomized basis, one treatment per pig. following this, a crude heat-stable enterotoxin (st) preparation produced from e. coli no. 1261 was injected into the proximal jejunal loop, and trypticase soy broth (tsb) (with osmolality adjusted to equal the enterotoxin preparation) was injected into the distal ... | 1985 | 4020946 |
| effect of virus-induced destruction of villous epithelium on intestinal secretion induced by heat-stable escherichia coli enterotoxins and prostaglandin e1 in swine. | villous atrophy and crypt hyperplasia were induced in the jejunal epithelium of thirteen 3-week-old pigs by inoculation with transmissible gastroenteritis virus. the responses (changes in net fluid movement) induced in ligated intestinal loops of these pigs by intraloop injections of prostaglandin e1 (pge1) or escherichia coli broth culture filtrates containing either or both e coli heat-stable enterotoxins (sta and stb) were compared with the responses induced by these preparations in littermat ... | 1985 | 2986497 |
| coliform numbers in the stomach and small intestine of healthy pigs following weaning at three weeks of age. | in 60 weaned and 52 unweaned pigs, aged between 21 and 32 days, the number of coliform organisms in the gastric contents was found to be related to their ph value. the contents were generally more acidic in the weaned animals and contained fewer coliforms. haemolytic coliform organisms were never identified among selected isolates examined from the gastric contents although in weaned animals the haemolytic enterotoxigenic escherichia coli serotype 0149: k91, k88a,c (abbotstown strain) was common ... | 1985 | 2993375 |
| [nature and incidence of carbadox resistance of escherichia coli isolated from fattening swine, calves and poultry]. | 1985 | 2861657 | |
| mannose-specific and hydrophobic interaction between escherichia coli and polymorphonuclear leukocytes--influence of bacterial culture period. | the influence of culture period on mannose-specific and hydrophobic properties of the bacterial surface and on bacteria/polymorphonuclear leukocyte (pmnl) interaction was studied. four e. coli strains, pn7 (01:k1), abu2 (on:k14), cu9 (06:k14) and cu13 (08:kn) and two salmonella typhimurium strains 395 mr10 and 395 ms, well characterized according to physicochemical surface properties, presence of type 1 fimbriae and interaction with pmnl, were used in the study. the results show that with prolon ... | 1985 | 2861706 |
| application of the peroxidase-antiperoxidase technique for the detection of enterotoxigenic escherichia coli k99 pilus antigen in paraffin-embedded tissues. | the peroxidase-antiperoxidase (pap) technique was evaluated as a method for detecting enterotoxigenic escherichia coli (etec) k99 pilus antigen in paraffin-embedded tissues of swine. antigenic reactivity was optimal when tissues were processed in cold methanol according to a modified method of sainte-marie. this method of processing adequately preserved tissue morphology. the pap technique was applicable to tissues where endogenous peroxidase activity was minimal. | 1985 | 2862183 |
| enterococci in lambs. | 1985 | 2862641 | |
| pili of enterotoxigenic escherichia coli from pigs and calves. | 1985 | 2866678 | |
| calcium mediation of the pig jejunal secretory response. | the involvement of ca++ ions as secretory mediators in pig jejunal epithelia has been investigated with an in vitro system. omission of ca++ from the ringer-hco3 bathing media on both sides of the tissue had minor effects on the basal electrical activity of pig jejunal mucosa. there were only slight decreases in transepithelial potential difference and increases in conductance with ca++ free media. low egta concentrations which reversibly blocked potential difference responses to secretory agent ... | 1985 | 2410089 |
| nucleotide sequences of four variants of the k88 gene of porcine enterotoxigenic escherichia coli. | the nucleotide sequences of four variants of the escherichia coli k88 antigen gene, k88ab1, k88ab2, k88ac, and k88ad, have been determined. the k88ab2 and k88ac sequences have not been reported previously. the k88ab1 sequence is very similar to that determined by other workers, but the k88ad sequence differs considerably from that described in a previous report. comparison of the amino acid sequences inferred from the gene sequences revealed certain clusters of amino acid substitutions which hav ... | 1985 | 2412961 |
| characterization of enterotoxigenic escherichia coli. serotypes, enterotoxins, adhesion fimbriae, and the presence of plasmids. | enterotoxigenic escherichia coli of human and procine origin were characterized with respect to their o and h antigens, fimbrial antigens, and type of enterotoxin produced. enterotoxin production was determined by bioassay (infant mice) and enzyme-linked immunoassay (elisa). the presence of genes coding for the enterotoxins was determined by dna-dna hybridization. the number and molecular size of plasmids in the enterotoxigenic strains were determined by gel electrophoresis. strains with the sam ... | 1985 | 2413710 |
| a comparison of methods for demonstrating colonization in the small intestine of piglets by enterotoxigenic escherichia coli. | 1985 | 2415207 | |
| new aspects of vaccine development. | 1985 | 2417761 | |
| behaviour of campylobacter sputorum subspecies mucosalis in gnotobiotic pigs. | gnotobiotic pigs were dosed orally with campylobacter sputorum subspecies mucosalis, either alone, or combined with rotavirus or non-pathogenic escherichia coli and streptococcus bovis to study the behaviour of c s mucosalis in defined conditions, to assess intracellular parasitism of enterocytes by c s mucosalis, and if possible to establish an experimental model of porcine intestinal adenomatosis. c s mucosalis colonised the gut of gnotobiotic pigs, persisting for up to 47 days after infection ... | 1984 | 6087431 |
| [the development of antibiotic resistance of coliform bacteria in connection with the nutritional use of nourseothricin in swine]. | 1984 | 6099110 | |
| recovery of intestinal membrane binding sites for k88 e. coli from pig mucosal organ cultures. | putative receptors for k88 + e. coli from piglet intestinal epithelium were released into the organ culture medium and were demonstrated by direct binding with k88 + e. coli through the utilization of an in vitro binding procedure or by immunoprecipitation with k88 antigen. incorporation of 14c-glucosamine by newborn to day old and 3-week to 6-week old piglet jejunal and ileal mucosa, in organ culture, occurred throughout the 24 hr culture period. uptake in both age groups and both areas of the ... | 1984 | 6146093 |
| effect of bambermycins, in vitro, on plasmid-mediated antimicrobial resistance. | the potential of bambermycins (a growth-promoting antimicrobial approved for turkeys, broilers, and swine) to overcome or control plasmid-mediated antimicrobial resistance was determined in a series of in vitro experiments. four possible modes of action of bambermycins were studied: synergistic effect with 12 other antimicrobials, elimination of resistance (r) plasmids from escherichia coli, selective killing or inhibition of e coli carrying r plasmids, and inhibition of r plasmid transfer. bamb ... | 1984 | 6151815 |
| [trends in drug therapy: electolyte transport in the intestines. the pathophysiology and therapy of enterotoxin-caused diarrheas]. | 1984 | 6375654 | |
| [amino acid composition of the alpha-ketoglutarate dehydrogenase from the breast muscle of the pigeon]. | the amino acid composition of alpha-ketoglutarate dehydrogenase--a component of alpha-ketoglutarate dehydrogenase complex--from the pigeon breast muscle has been determined. a significant similarity of the enzyme amino acid composition isolated from systematically remote species has been revealed by comparison of the data obtained with the literary one concerning the alpha-ketoglutarate dehydrogenase from escherichia coli and pig heart. | 1984 | 6375738 |
| antimicrobial resistance and conjugative r plasmids in escherichia coli strains isolated from animals in peninsular malaysia. | fifteen independent e. coli strains of avian, bovine and porcine origin in peninsular malaysia were tested for antibiotic resistance and conjugative r plasmids. eight (53%) isolates were found to be antibiotic resistant. among them, 37.5% were mono-resistant and 62.5% were resistant to three or more antibiotics, i.e., multi-resistant. all of them were resistant to tc and sensitive to gm and nx. three of the eight antibiotic resistant strains were able to transfer all or part of their resistance ... | 1984 | 6377513 |
| divergent patterns of leucocyte locomotion in experimental post-traumatic osteomyelitis. | leucocyte locomotion (ll) was evaluated in guinea-pigs with experimental post-traumatic osteomyelitis. surgical procedure (fracture of the right femur), the number of micro-organisms applied to the fractured site and the time sequence of investigations were kept constant. after the right femur of guinea-pigs had been fractured, the animals were divided into three groups: in group i the fractured site remained uninfected, while in groups 2 and 3 the fractured sites were infected with either esche ... | 1984 | 6378240 |
| attachment of human and pig (k88) enterotoxigenic escherichia coli strains to either human or porcine small intestinal cells. | enterotoxigenic escherichia coli strains pathogenic for humans and enterotoxigenic e. coli strains pathogenic for pigs producing the k88 pili antigen both bound to isolated small intestinal cells from either humans or pigs. neither the k99 enterotoxigenic e. coli (from lambs and calves) nor the rabbit pathogenic strain rdec-1 bound to human or pig small intestinal cells under the same conditions. | 1984 | 6378801 |
| naloxone therapy in awake endotoxemic yucatan minipigs. | twelve yucatan miniature pigs were fitted with jugular, portal, hepatic vein, and carotid artery catheters, and hepatic artery and portal vein flow cuffs to quantitate portosystemic and transhepatic kinetics. seventy-two hours later they were placed in slings, and following a 3-hr control period were infused with escherichia coli endotoxin at 15 micrograms/kg/hr for 6 hr. eight were controls and 4 received a primed (2 mg/kg) continuous infusion (2 mg/kg/hr) of naloxone 1 hr after initiation of e ... | 1984 | 6379299 |
| ferritin: protection of enzymatic activity against the inhibition by divalent metal ions in vitro. | ferritin binds a large quantity of be2+ (price d.j. and joshi, j.g. j. biol. chem. 258 (1983) 10873) as well as other divalent metal ions. therefore the ability of this protein to protect enzymes against or reverse the inhibition by metal ions was studied. evidence presented here shows that the inhibition by be2+ of the enzymes na+k+atpase, alkaline phosphatase and phosphoglucomutase is reversed by ferritin. be2+ can be transferred reversibly between phosphoglucomutase and ferritin depending upo ... | 1984 | 6330934 |
| role of peripheral pooling in porcine escherichia coli sepsis. | in anesthesized pigs the effects of e. coli (2 x 10(8)/kg) on hemodynamics and red cell distribution were studied. after injection of 99m-tc red cells (15 mci), regional radioactivity was followed during 3 hours. gated bloodpool studies were performed to measure end-diastolic volumes (edv). escherichia coli e. coli was infused in 14 pigs, while 7 animals served as controls. e. coli resulted in an early increase in pulmonary arterial pressure. systemic arterial pressure decreased gradually, while ... | 1984 | 6368039 |
| effect of bacterial immunization on experimental reflux nephropathy. | this study was designed to test the hypothesis that young animals with vesicoureteral reflux might be more vulnerable to renal parenchymal infection by bacteria to which they had not been previously exposed. forty-four crossbred male piglets had surgical induction of vesicoureteral reflux at 2 weeks of age and introduction of urinary tract infection at 6 weeks. they were sacrificed at 12 weeks of age. between the ages of 2 and 6 weeks, 22 piglets received subcutaneous injections of formalin-kill ... | 1984 | 6368870 |
| relationship of iron administration to susceptibility of newborn pigs to enterotoxic colibacillosis. | to determine whether supplemental iron (fe) administration to newborn pigs reared in concrete pens not only prevents anemia, but renders the pigs more susceptible to escherichia coli-induced diarrheal disease, pigs were given a large or a small dose of fe im or orally before or after challenge exposure with e coli. the controls were challenge-exposed pigs not given fe and pigs not challenge exposed (fe-treated and nontreated groups). although the mortality of the pigs challenge exposed with e co ... | 1984 | 6370051 |
| enteric diseases in animals caused by escherichia coli: their control and prevention. | 1984 | 6373433 | |
| prevention of colibacillosis in neonatal swine with a 4-pilus e coli bacterin. | of 12 pregnant swine (vaccinates) given a 4-pilus enterotoxigenic e coli (etec) bacterin (k88, k99, 987p, f41), all developed comparable or significantly higher serum and colostral antibody levels than those of 8 pregnant swine (controls) given a 3-pilus etec bacterin (k88, k99, 987p). when piglets were challenged with an etec strain bearing the f41 antigen, those from vaccinates had significantly lower mortality, less scours, less severe clinical signs and better weight gain than those from con ... | 1984 | 6374422 |
| the reaction of pyridoxal phosphate with holoenzymes in the pyridoxamine phosphate form. | 1984 | 6374674 | |
| [immunogenesis of 2 live vaccines against swine colibacillosis]. | comparative testing was carried out on a farm with 2 live vaccines against coli bacteriosis . studied were blood and milk sera taken from test and control pigs and blood sera from pigs born by them. both the test and control groups were clinically followed up with recording the morbidity and mortality indices. it was found that the titer of the k88 antibodies rose after vaccination with the two vaccines and retained its level up to the 30th day after farrowing. the titer of the o antibodies rema ... | 1984 | 6375113 |
| [properties of e. coli isolated from pigs]. | a total of 127 strains of escherichia coli were isolated from the small intestine, mesenterial lymph nodes, and parenchymal organs from dead pigs or pigs that were killed for diagnostic purpose as well as from rectal swab samples taken from diseased or clinically normal animals. all were studied with regard to haemolytic properties via biochemical and serologic methods along for sensitivity to antibiotics. it was found that the isolated strains manifested characteristic biochemical behaviour (79 ... | 1984 | 6375116 |
| [changes in the white blood picture and the t- and b-lymphocyte counts in the peripheral blood of pigs after treatment with e. coli endotoxin]. | studied were the changes in the total count of the white blood cells, the leukocyte formula, and the t- and b-lymphocytes of young pigs (2-3 months old) at various intervals following treatment with an e. coli enterotoxin. it was found that there set in an essential drop of the total count of the white blood cells during the first 24 hours after treatment, with a following rising trend. there were also characteristic changes in the ratio between the various types of white blood cells, with a ris ... | 1984 | 6388144 |
| does enteropathogenic escherichia coli produce heat-labile enterotoxin, heat-stable enterotoxins a or b, or cholera toxin a subunits? | although most enteropathogenic escherichia coli strains do not produce recognized enterotoxins, we wished to examine whether they produce any factors like heat-stable enterotoxin b or cholera toxin active subunits that might be missed by conventional assay methods. e. coli strains e851 (o142) and e2348 (o127) that had caused diarrhea in volunteers were negative for heat-labile enterotoxin and heat-stable enterotoxin a in chinese hamster ovary cell and suckling mouse assays, failed to cause secre ... | 1984 | 6389354 |
| persistent hyperinsulinemia due to induced euglycemia in awake endotoxic minipigs. | transient hyperinsulinemia has been incriminated as a contributing factor to endotoxin-induced hypoglycemia. however, a recent study using endotoxic minipigs noted an increase in the rate of glucose utilization prior to when hyperinsulinemia was seen. based on this, the "misinformed beta-cell" hypothesis was proposed. if endotoxic pancreatic beta-cells experience an early increase in glucose uptake, they could over-estimate the true glycemic state and release insulin, further contributing to the ... | 1984 | 6391719 |
| significance of thermostable enterotoxin produced by porcine enteropathogenic escherichia coli. | 1984 | 6393557 | |
| portal and systemic plasma vip and secretin during intravenous infusion of escherichia coli endotoxin in pigs. | plasma vasoactive intestinal polypeptide (vip) increased significantly in the portal vein, the superior caval vein, the internal jugular vein, and the aorta during an intravenous infusion of e. coli endotoxin in six anesthetized pigs, which all developed a deep shock, with fall in aortic blood pressure and urinary output. plasma vip peaked simultaneously with the most pronounced shock symptoms, and the level in the portal vein was significantly higher than in blood from the other sampling sites. ... | 1984 | 6398505 |
| escherichia coli originated from diarrhea of suckling piglets in taiwan. ii. serological properties. | 1984 | 6399334 | |
| [a possible way of improving colibacterin]. | 1984 | 6401093 | |
| f41 antigen among porcine enterotoxigenic escherichia coli strains lacking k88, k99, and 987p pili. | colonial morphological variations among enterotoxigenic escherichia coli which lack k88, k99, and 987p (3p-) were shown to be correlated with expression of several surface antigens, i.e., type 1 pili, f41 pili, and somatic and capsular antigens. such correlations were established by electron microscopy, serology, and hemagglutination of cells derived from these specific colonial types. identification of f41 produced by the 3p- enterotoxigenic e. coli strains was made by immunodiffusion in agaros ... | 1984 | 6420345 |
| [phospholipase activity of the membrane fraction of phagocytes in relation to its antibacterial property]. | 1984 | 6436567 | |
| therapeutic efficacy of a new cephamycin, mt-141, in compromised mice. | the antibacterial activity of mt-141 against escherichia coli and proteus morganii in compromised mice was investigated and compared with that of latamoxef, cefmetazole and cefoxitin. the bactericidal activity of mt-141 in short-term contact with e. coli and p. morganii was markedly enhanced when combined with mouse serum, and the activity of mt-141 was greater than the activities of the three reference drugs. the antibacterial activities of mt-141 in the liver, spleen and kidney of neutropenic ... | 1984 | 6441793 |
| pathomorphology and pathogenesis of bacterial meningoventriculitis of neonatal ungulates. | bacterial meningoventriculitis was studied in 26 neonatal ungulates. preceded by a substantial bacteremia, usually due to escherichia coli, the fibrinopurulent inflammation involved leptomeninges, choroid plexuses, and ventricle walls, but largely spared the neuraxial parenchyma. it is proposed that this surface-relatedness results from the transport of bacteria by monocytes of low bactericidal power, migrating by normal pathways to maintain significant surface populations of macrophages. the ne ... | 1984 | 6516179 |
| [pharmacological, toxicological, microbiological and pathohistological studies of gentamycin complex ii]. | the acute toxicity was determined of the gentamycin complex ii (gmc-ii) for albino mice, containing gentamycin components in a varying ratio. the ld50 at intraperitoneal application was 347.7 mg/kg body mass, while at oral application it was 5,180 mg (4933 divided by 5439) mg/kg body mass. histopathologically, (mice killed with high doses of gmc-ii, given orally) there were diffuse hyperemia of the liver, parenchymal degeneration of the kidney tubuli epithelium, and edema and emphysema of the lu ... | 1983 | 6623911 |
| [clinical, cytological and microbiological studies of the mma syndrome]. | clinical, cytological, and microbiological investigations were carried out of a total of 793 sows in the course of 10 days after farrowing. it was found that the mma syndrome was manifested in the first days following farrowing with higher temperature, changes in the genital organs with a purulent discharge from the first to the third day, and lesions in the mammary gland with inflammatory processes or decrease in the milk secretion. the cell content of milk of the diseased sows was higher. the ... | 1983 | 6659347 |
| safety evaluation of thaumatin (talin protein). | thaumatin, the sweet proteinaceous extract of the arils of thaumatococcus daniellii (benth.) has been studied for its subacute toxicity in rats and dogs and its ability to produce anaphylactic antibodies following oral administration to rats and normal human subjects. thaumatin was readily digested prior to absorption in rats and no adverse effects resulted from its continuous administration to rats and dogs at dietary concentrations of 0, 0.3, 1.0 and 3.0% for 13 wk. it was not teratogenic when ... | 1983 | 6686588 |
| dietary regimen, rotavirus, and hemolytic enteropathogenic escherichia coli in weanling diarrhea of pigs. | previously, we induced weanling diarrhea in piglets by infecting them first with rotavirus followed by a hemolytic enteropathogenic escherichia coli. we postulated that rotavirus, by damaging the epithelium of the small intestines, produced an entero-environment which favored the selection and growth of the enteropathogenic e. coli. furthermore, diet might affect the entero-environment and influence the course of the disease. to test this, newly weaned 3-week-old piglets were assigned to one of ... | 1983 | 6375527 |
| the role of dietary antigen in the aetiology of post weaning diarrhoea. | experiments were undertaken to investigate whether a hypersensitivity response to dietary antigen might be a predisposing factor in the aetiology of post weaning diarrhoea. the results indicated that: 1. very small amounts of food given to baby pigs before weaning significantly increased the severity and accelerated the onset of the disease; 2. post weaning diarrhoea was dependent upon the presence within the diet of antigenic material; 3. weaning diets containing little antigenic material had l ... | 1983 | 6375529 |
| drug resistance and conjugative r plasmids in fecal escherichia coli strains isolated from healthy younger animals (chickens, piglets, calves) and children. | a total of 11,777 escherichia coli strains were isolated from 90 chickens, 103 piglets, 96 calves, and 104 children in 1979 in gunma prefecture and tested for drug resistance and the presence of conjugative r plasmids. the percentages of individuals that excreted drug-resistant strains were: chickens, 100%; piglets, 99%; calves, 100%; and children, 64%. the frequency of isolation of drug-resistant strains among the total isolates was: chickens, 98%; piglets, 93%; calves, 94%; and children, 41%. ... | 1983 | 6374389 |
| study of several factors affecting the agglutinating activity of k99-positive escherichia coli strains. | the effect of several factors on escherichia coli k99-plasmid associated agglutination has been studied. the results obtained indicate that escherichia coli 637 (k99+) mediated red blood cell agglutination is unspecific although the agglutination titres for several erythrocyte species are significantly different. the agglutination is highly stable (at least with sheep red blood cells) to changes in temperature (from 4 degrees c to 37 degrees c), to changes in ph (from 5 to 9) and to the presence ... | 1983 | 6371953 |
| [dynamics of antibiotic resistance of strains of escherichia coli isolated from feces of weaned piglets (the case of the lwanika- lubumbashi (zaire) farm]. | 1983 | 6369447 | |
| heme-iron and ecology of escherichia coli within the porcine gut. | growth of escherichia coli was followed in sow whey by a turbidometric technique. heme-iron clearly promoted bacterial growth, therefore free heme would be dangerous in small intestine by enhancing bacterial growth: duodenal brush border membrane (bbm) was seen to bind heme-iron, thus abolishing the growth promoting effect of this form of iron. scatchard analysis of heme-binding onto bbm showed that heme became bound by a specific mechanism (kd approximately equal to 10(-7) m) as well as non-spe ... | 1983 | 6368116 |
| neonatal diarrhea of pigs in quebec: infectious causes of significant outbreaks. | to evaluate the relative importance of the various enteropathogens causing neonatal diarrhea in quebec farrowing operations, observations were made on 749 diarrheic pigs from 325 outbreaks of diarrhea. they were one to 15 days of age, and were obtained alive for necropsy generally within 48 hours of the onset of diarrhea. some pigs were from severe, explosive outbreaks of diarrhea with high morbidity and mortality rates, while others were from herds with chronic neonatal diarrhea with lower morb ... | 1983 | 6299483 |
| bacterial cell wall components decrease the number of guinea-pig lung beta-adrenoceptors. | infections of the deeper respiratory airways can contribute to the progression of chronic asthmatic bronchitis. in the present report a number of microorganisms affecting the number of beta-adrenoceptors in guinea-pig lung homogenates are described. haemophilus influenzae, streptococcus pneumoniae, bordetella pertussis and escherichia coli o111b4 induced a significant decrease of the number of beta-adrenoceptors (by approximately 20%). staphylococcus aureus, influenza a virus and escherichia col ... | 1983 | 6301848 |
| relatedness of chloramphenicol resistance plasmids in epidemiologically unrelated strains of pathogenic escherichia coli from man and animals. | i have examined 20 plasmids conferring chloramphenicol resistance (cm) in multiresistant strains of escherichia coli pathogenic for man and piglets. in denmark, one plasmid family, exemplar phg33, is responsible for all chloramphenicol resistance in serotypes of e. coli found in diseased piglets. a closely related plasmid, phg50, was identified in an enteropathogenic e. coli (epec) strain from an infant. the isolate was epidemiologically unrelated to the piglet isolates. the molecular relatednes ... | 1983 | 6302257 |
| interactions between entamoeba histolytica, bacteria and intestinal cells. | axenically grown pathogenic and non-pathogenic isolates of entamoeba histolytica have been shown to adhere to mammalian epithelial cells and bacteria by virtue of carbohydrate-binding proteins present on their cell surfaces. the interaction of amoeba isolates of low pathogenicity with a variety of gram-negative bacteria, mainly escherichia coli strains which are readily ingested by the amoebae after relatively short periods, significantly increased the ability of the trophozoites to: (a) destroy ... | 1983 | 6315320 |
| differential toxicity of inhaled gram-negative bacteria. | the toxicity by inhalation of various gram-negative bacteria, isolated from settings associated with inhalation disease, was studied by a variety of means. these microorganisms were not equally toxic. citrobacter freundii aerosol challenges of rabbits provoked significant (up to fivefold) increases in plasma haptoglobin 24 to 48 h after inhalation. other strains tested failed to provoke such statistically consistent increases. measurements of c-reactive protein in these same animals did not lead ... | 1983 | 6339400 |
| an in vitro system to study interactions between bacteria and epithelial cells at the molecular level. | this paper describes an experimental system to study interactions between porcine enterotoxigenic escherichia coli (etec) and porcine intestinal epithelial cells in vitro at the molecular level. radiolabelled bacteria or bacterial membrane fractions were incubated with brush borders prepared from purified epithelial cells, which were then washed repeatedly. the bacterial components removed by washing or retained by the brush borders were analysed to determine their composition and source. for th ... | 1983 | 6339675 |
| gastric mucosal blood flow and escherichia coli bacteremia. | the specific changes in gastric blood flow during sepsis are controversial. previous investigations of intragastric blood flow using endotoxin models revealed decreased total gastric blood flow and decreased gastric mucosal blood flow. endotoxin models are now thought to be inadequate due to the accompanying depression of the systemic circulation. more recently, increased gastric blood flow has been demonstrated in a septic hindlimb model. to further elucidate the changes in intragastric blood f ... | 1983 | 6340545 |
| partial purification and characterization of an escherichia coli toxic factor that induces morphological cell alterations. | a factor produced by several strains of escherichia coli isolated from enteritis-affected children has been shown to produce both a necrotizing effect on rabbit skin and striking morphological alterations on cho, vero, and hela cells. the same strains were found to have hemolytic activity on sheep erythrocytes. the toxic, cell-altering factor was demonstrated to be different from both heat-labile and heat-stable enterotoxins and from vero toxin. the main effect induced by the isolated factor on ... | 1983 | 6341235 |
| from the national institute of allergy and infectious diseases. summary of a workshop on enteropathogenic escherichia coli. | 1983 | 6343525 | |
| drug resistance and r plasmids in escherichia coli isolated from laboratory animals. | 1983 | 6345875 | |
| acid-base and electrolyte changes in 1-3 days old piglets infected with enteropathogenic escherichia coli and in spontaneous cases of piglet diarrhoea. | 1983 | 6346838 | |
| vaccination of piglets against post-weaning "e. coli" enterotoxicosis. | e. coli enterotoxicosis is one of the main causes of neonatal diarrhea in piglets. for their protection against this disease a vaccine has been prepared based on k88 and lt. pregnant sows were vaccinated, thereby transferring passive immunity to their offspring through colostrum and milk. protection was obtained in terms of decrease in mortality, occurrence of diarrhea and excretion of enteropathogenic bacteria. piglets from non-immune sows could be immunized shortly after birth. however, piglet ... | 1983 | 6347759 |