Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| clinical and pathological evaluation of sulbactam/ampicillin for treatment of experimental bovine pneumonic pasteurellosis. | an experiment was conducted to evaluate the efficacy of sulbactam/ampicillin for treatment of bovine pneumonic pasteurellosis. twenty-one hereford calves were experimentally infected with bovine herpesvirus-1 and an ampicillin-resistant strain of pasteurella haemolytica, then treated for three days with either sulbactam/ampicillin, chloramphenicol, or a placebo. the treatments were evaluated by comparing clinical illness scores, total sick days, weight changes, mortality rates, and postmortem lu ... | 1988 | 17422967 |
| the in vitro exposure to bovine rhinotracheitis virus of zona pellucida-micromanipulated bovine embryos with the zona pellucida damaged or removed. | one hundred and eighty-five embryos were collected from 29 superovulated donors 6 to 8 d post estrus. the zona pellucida (zp) of these embryos was either cracked, removed mechanically or removed with acidified tyrode's solution, or left intact. forty-eight of 103 (47%) zp-cracked and zp-free embryos, exposed for 24 h to infectious bovine rhinotracheitis virus (ibrv), survived. no significant difference was found in the embryonic survival of the zp-cracked embryos exposed to ibrv and control embr ... | 1987 | 16726332 |
| t lymphocyte population dynamics and function following a primary bovine herpesvirus type-1 infection. | following a primary bovine herpesvirus-1 (bhv-1) infection the concanavalin a (con a) induced proliferative responses of peripheral blood t lymphocytes were suppressed. this suppression occurred in the absence of detectible serum suppressor factors, suppressor cell activity or decreased accessory cell function. however, regression analysis demonstrated a significant correlation between the percentage of t lymphocytes present within the peripheral blood mononuclear cell (pbmc) population and the ... | 1987 | 3509949 |
| efficacy of a streptomycin-dependent, live pasteurella haemolytica vaccine against challenge exposure to pasteurella haemolytica in cattle. | a streptomycin-dependent, live pasteurella haemolytica vaccine was given in 1 or 2 doses to 2 groups of weaned calves; 2 other groups of calves were not vaccinated. all calves in the vaccinated groups and calves in 1 of the nonvaccinated groups were stressed by transport, intratracheally inoculated with bovine herpesvirus type-1 (cooper strain), and then intratracheally inoculated with p haemolytica type a1. the 4th group of calves (nonvaccinated controls) was not stressed and were not intratrac ... | 1987 | 3296883 |
| effects of infectious bovine rhinotracheitis virus infection on bovine airway reactivity. | the response of isolated tracheal and bronchial strips to isoproterenol in vitro was studied in eleven male jersey calves. clinical, microbiological and pathological evaluations of the calves were carried out. in calves exposed once or twice to infectious bovine rhinotracheitis virus, the relaxation threshold of the trachealis muscle to isoproterenol was significantly (p less than 0.05) impaired (threshold 5.0 x 10(-7) m, single exposure and 1.0 x 10(-7) m, double exposure), when compared with u ... | 1987 | 3651887 |
| effect of dexamethasone on bovine leukocyte functions and bovine herpesvirus type-1 replication. | in an attempt to elucidate the mechanism whereby dexamethasone could reactivate bovine herpesvirus type-1 the effect of dexamethasone on virus replication and leukocyte functions was assessed. no effect was detectable on either virus yield or in vitro replication kinetics. in contrast, dexamethasone influenced several leukocyte functions thought to be of importance in antiviral defense and maintenance of latency. in vitro exposure of peripheral blood polymorphonuclear neutrophilic granulocytes o ... | 1987 | 3651888 |
| intracellular localization and transport of three different bovine herpesvirus type 1 glycoproteins involved in neutralization. | monoclonal antibodies against 3 different glycoproteins of bovine herpesvirus type 1 (bhv-1) involved in virus neutralization were used in indirect immunofluorescence (iif) tests to characterize the appearance and transport to the plasma membrane of virus antigens in the infected cells. antibodies against gp 117 and gp 71 glycoproteins first showed pronounced ring-like nuclear fluorescence at 4 hours post-infection (pi), followed by staining of the perinuclear region, presumably the golgi appara ... | 1987 | 3026288 |
| bovine peripheral blood leukocyte subpopulation dynamics following a primary bovine herpesvirus-1 infection. | population dynamics of bovine peripheral blood leukocyte subpopulations were quantitated following a primary bovine herpesvirus-1 (bhv-1) infection. percoll isolated peripheral blood mononuclear cell (pbmc) subpopulations were analyzed using flow cytometry (fc) and cytochemical stains. between days two to eight post-infection (pi) there was a significant decrease in the percentage of t-cells and nont/nonb cells which was accompanied by an increased percentage of b-cells and monocytes. these perc ... | 1987 | 3509948 |
| [evaluation of synthetic activity of lymphocyte nucleoli and phagocytic activity in peripheral blood leukocytes in heifers after infection with infectious bovine rhinotracheitis virus]. | the biosynthetic activity of the nucleoli of peripheral blood lymphocytes in calves, and the proportion of lymphocytes with the micronucleoli, with compact and ring-shaped nucleoli, were evaluated after experimental intranasal and intratracheal infections with the infectious bovine rhinotracheitis (ibr) virus. ibr virus belongs to the group of herpes viruses, the control of which is dominated by cellular immunity. the results are compared with the values for phagocytizing neutrophils, phagocytic ... | 1987 | 3122402 |
| serologic survey of viral antibodies in the peruvian alpaca (lama pacos). | sera from more than 100 alpacas (lama pacos) from the peruvian southern sierra were examined for antibodies to 8 viruses known to infect other domestic animals. on the basis of these serologic findings and previously published serologic or clinical data, it is now known that the alpaca can be infected with the following viruses: parainfluenza-3, bovine respiratory syncytial virus, bovine herpesvirus-1, bluetongue virus, border disease virus, influenza a virus, rotavirus, rabies virus, vesicular ... | 1987 | 3826854 |
| highly sensitive antigen detection procedures for the diagnosis of infectious bovine rhinotracheitis: amplified elisa and reverse passive haemagglutination. | the sensitivity of an enzyme-linked immunosorbent assay (elisa) for the detection of bovid herpesvirus 1 antigen was increased by up to 50-fold using the biotin-avidin interaction to amplify the reaction, when compared with a simple sandwich elisa. an alternative immunoassay, reverse passive haemagglutination (rpha), had a similar sensitivity to the amplified elisa, and was technically simpler to perform. both the amplified elisa and the rpha could detect viral antigen in the nasal secretions of ... | 1987 | 3031873 |
| early embryonic death in heifers after inoculation with bovine herpesvirus-1 and reactivation of latent virus in reproductive tissues. | thirteen crossbred heifers seronegative for bovine herpesvirus-1 (bhv-1) were bred naturally to a seronegative bull. eight heifers were inoculated with bhv-1, iv, on postbreeding day (pbd) 7 or 14. viremia was detected in heifers 1 through 7, and virus also was isolated from nasal and vaginal secretions of heifers 2, 3, 4, 6, and 7. the pregnancy status of all heifers was monitored from pbd 14 to pbd 35 by determining plasma progesterone concentrations at 1- to 3-day intervals. decreased progest ... | 1987 | 3434897 |
| [bhv-1 infection of cattle: stabilization by active immunization of seropositive animals with inactivated vaccine--a field trial]. | 1987 | 3040371 | |
| comparison of five cell lines for the propagation of bovine viral diarrhea and infectious bovine rhinotracheitis viruses. | a comparative study was carried out to determine the susceptibility of 5 different cell types to bovine viral diarrhea (bvd) and infectious bovine rhinotracheitis (ibr) viruses. the cell systems used were swine testicle (st), mink lung (ml), bovine turbinate (bt), porcine kidney (pk15) and equine dermal (ed) cells. for bvd virus, the titers obtained on day 8 post-infection were 10(1.13), 10(3.25), 10(4.13), 10(0.00) and 10(0.00), in st, ml, bt, pk15 and ed cells, respectively. for the ibr virus, ... | 1987 | 3041184 |
| inheritance and interaction of immune traits in beef calves. | three sets of blood samples were obtained from beef calves of two experimental populations and assayed for various immunological measurements. the first set of samples was taken between 24 and 48 h after birth and quantified for igg1 concentration. a second set was taken immediately prior to vaccination for infectious bovine rhinotracheitis virus (ibrv; at an average age of 164 d) and a third set taken 60 d post-vaccination. these later samples were quantified for antibodies specific to ibrv. le ... | 1987 | 3030992 |
| hemagglutination inhibition and virus neutralizing response of rabbits inoculated with bovine herpesvirus 1 subunit vaccine. | the immunogenicity of bovine herpesvirus type 1 (bhv-1) hemagglutinin has been investigated. both live and nonionic detergent solubilized vaccines were prepared and 5000 hemagglutinating units (hau) were injected subcutaneously into rabbits. both types of vaccine induced a good antibody response but live virus was four times more efficient in inducing hemagglutination inhibiting and neutralizing antibodies than either triton x-100- or octylglucoside-solubilized subunit vaccine. blotting analysis ... | 1987 | 3031871 |
| reactivation in calves of latent infection by bovid herpesvirus-4. | nine calves, six of which had been infected with strain 85/bh 16tv and three with strain 85/bh 232tn of bovid herpesvirus-4 (bhv-4), were treated with dexamethasone (dms) three months after infection. dms administration did not induce any clinical signs of disease, but bhv-4 was isolated from the nasal swabbings of all calves for a maximum of 8 days after the start of dms treatment. the virus was also isolated from the nerve tissues, nasal mucosa, lymph nodes, lung and spleen of 4 calves that we ... | 1987 | 3033450 |
| reactivation of infectious bovine rhinotracheitis virus by transport. | transport was studied as a cause of reactivation of infectious bovine rhinotracheitis virus (bovine herpesvirus-1; bhv-1) in heifers vaccinated 2-6 months before transport, using a double dose of the thermosensitive (ts) vaccine strain (tracherine). eight out of 19 animals showed ts strain re-excretion over a period of 1-3 days, beginning, in 5 out of the 8 heifers, the day after transport. in 14 other heifers, only sera were examined by sero-neutralisation: only 1 out of these 14 animals showed ... | 1987 | 3034502 |
| comparison of stationary and roller cultures for the isolation of herpesviruses affecting livestock. | the effect of rotating cultures on the isolation and cultivation of pseudorabies, infectious bovine rhinotracheitis and equine rhinopneumonitis viruses was studied. serial ten-fold dilutions of laboratory strains of these viruses and field strains of pseudorabies virus were inoculated in two each of roller and stationary tubes of appropriate cell cultures. all tubes were examined daily for the appearance of cytopathic effects. roller cultures were found to slightly enhance the speed and extent o ... | 1987 | 3035348 |
| the genome of caprine herpesvirus 1: genome structure and relatedness to bovine herpesvirus 1. | caprine herpesvirus 1 (caphv-1) dna was examined by electron microscopy, restriction site mapping and homology studies with bovine herpesvirus 1 (bhv-1) dna. although the restriction site maps differed significantly, we showed that the genome structures of caphv-1 and bhv-1 were identical and that the dnas shared a high degree of base sequence homology. | 1987 | 3037021 |
| protection of cattle from bovine herpesvirus type i (bhv-1) infection by immunization with individual viral glycoproteins. | the major glycoproteins gi, giii, and giv of bovine herpesvirus-1 (bhv-1) were found to induce high levels of antibody in cattle which could neutralize virus and participate in antibody-dependent cell cytotoxicity of bhv-1-infected cells. immunized animals were fully protected from disease, using a bhv-1/pasteurella haemolytica aerosol challenge model but not from infection with the virus. thus, virus could still replicate in the nasal passages of immunized animals, although to a lesser extent t ... | 1987 | 3037783 |
| detection of bovine herpesvirus-1 nucleic acid sequences, using a dot-blot hybridization procedure. | a sensitive and specific procedure for the detection of argentine isolates of bovine herpesvirus-1 was developed. the procedure was based on a dot-blot, nucleic acid hybridization, using 32p, nick-translated, plasmidic probes. the probes contained cloned bam h1 restriction fragments in the left half of the viral genome. the detection limit of the procedure was 10 pg of viral dna. | 1987 | 3037953 |
| vaccination studies against experimental bovine pasteurella pneumonia. | vaccination-challenge experiments were conducted in colostrum-deprived calves to evaluate the efficacy of pasteurella bacterins and vaccines against experimental pneumonic pasteurellosis. calves were vaccinated with formalin-killed bacterins and live vaccines, then challenge exposed intratracheally with p. haemolytica or p. multocida. infectious bovine rhinotracheitis virus was inoculated intranasally three to four days prior to p. haemolytica challenge-exposure. all calves were examined for mac ... | 1987 | 3300921 |
| use of recombinant bovine alpha 1 interferon in reducing respiratory disease induced by bovine herpesvirus type 1. | intranasal or intramuscular treatment of calves with recombinant bovine alpha 1 interferon before challenge with bovine herpesvirus type 1 and pasteurella haemolytica reduced clinical signs, number of sick days, lung lesions, and weight loss. the effective dose was determined to be relatively broad within the range of 1 to 50 mg per animal. no adverse effects were observed even at high doses of interferon (50 mg per animal). administration before virus infection was more effective than administr ... | 1987 | 3038008 |
| viral-bacterial pneumonia in calves: effects on plasma eicosanoids and long chain fatty acids. | in the pathogenesis of bovine pneumonic pasteurellosis, immunodepression induced by stress or respiratory viral infection permits superinfection of the lungs with pasteurella hemolytica, which results in exudative fibrinous pneumonia. therefore, bovine pneumonic pasteurellosis was induced by sequential inoculations of calves with bovine herpes virus-1 (bhv-1, 3 x 10(7) tissue culture infectious dose 50 (tcid50)/nostril), followed 3 days later by challenge with p. hemolytica (15 x 10(9) colony-fo ... | 1987 | 3036736 |
| elisa for bovine herpesvirus 1 (bhv1) antibody with hirt supernatant. | detailed studies were made of the ability of hirt supernatant (hs) from bovine herpesvirus 1 (bhv1) infected cultures to sensitize plates for enzyme-linked immunosorbent assay (elisa). the ultracentrifuged pellet of hs had less sensitizing activity than the supernatant, but the antigen was removed completely by 0.22 micron filters and to some extent by 0.45 micron filters; it was minimally affected by sonication; it was destroyed by the action of pronase but not by dnaase i when a kallikrein ina ... | 1987 | 3038916 |
| comparative efficacy of three 2'-fluoropyrimidine nucleosides and 9-(1,3-dihydroxy-2-propoxymethyl)guanine (bw b759u) against pseudorabies and equine rhinopneumonitis virus infection in vitro and in laboratory animals. | the three 2'-fluoropyrimidine nucleosides 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine (fiac), 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodouracil (fiau), and 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-methyluracil (fmau), showed high activity in rk13 monolayers against equine rhinopneumonitis virus, (ehv-1, ic50 range 0.02-0.18 microm), aujeszky's disease virus (shv-1, pseudorabies, ic50 range 0.25-7 microm) and infectious bovine rhinotracheitis virus (1br, bhv-1, ic50 ... | 1987 | 3026244 |
| reactivity of monoclonal antibodies to proteins of a neurotropic bovine herpesvirus 1 (bhv-1) strain and to proteins of representative bhv-1 strains. | 15 monoclonal antibodies (mcabs) were induced with the neuropathogenic strain n 569 of bovine herpesvirus 1 type 3 (bhv-1.3). nine of them could be shown by radioimmunoprecipitation assay to react with viral glycoproteins and two of these mcabs were able to neutralize strain n 569. the reactivity of these 15 monoclonals was compared with 11 monoclonal antibodies induced with a bhv-1.1 strain. the available monoclonal antibodies made it possible to characterize bhv-1.3 and to classify bhv-1 into ... | 1987 | 3034198 |
| natural cell-mediated cytotoxicity to cells infected with infectious bovine rhinotracheitis virus. | cell-mediated cytotoxicity against viral-infected cells was demonstrated in a 6-hr 51cr release assay. peripheral blood mononuclear leukocytes from both infectious bovine rhinotracheitis virus (ibrv)-infected and noninfected cattle exhibited preferential lysis against ibrv-infected primary bovine embryonic kidney (bek) cells compared to cells infected with pseudorabies virus and noninfected bek cells. addition of specific antibody to the assay did not enhance cytotoxicity. the effector cell was ... | 1987 | 3029944 |
| influence of isoprinosine on bovine herpesvirus type-1 infection in cattle. | a study was conducted to determine the in vivo efficacy of isoprinosine (iso) in calves infected with bovine herpesvirus type-1 (bhv-1). calves were infected with bhv-1 on day 0 and received iso daily for 14 days. clinical signs of disease, shedding of bhv-1, lymphocyte proliferative responses to mitogens, interleukin-2 production, and alveolar macrophage bactericidal activity were monitored during the study. rectal temperatures were increased (p less than 0.05) in bhv-1 and iso-bhv-1 calves at ... | 1987 | 2442882 |
| temporal control of bovine herpesvirus 1 glycoprotein synthesis. | the gi, giii, and giv glycoproteins are major bovine herpesvirus 1 antigens involved in virus neutralization. results indicate that the gi and giv glycoproteins were expressed as beta proteins, whereas the giii glycoprotein was expressed strictly as a gamma protein. these findings suggest that gi and giv may be superior to giii as vaccine candidates. | 1987 | 2442412 |
| vaccination of rabbits with a bovine herpesvirus type 1 subunit vaccine: adjuvant effect of iscoms. | bovine herpesvirus type 1 has two major immunogenic surface glycoproteins: a 90 kda haemagglutinin and the 130 (74 + 54) kda glycoprotein. these proteins were purified by rate zonal sucrose density gradient centrifugation after extraction with nonionic detergent triton x-100. for the preparation of iscoms, the glycoproteins were further adsorbed during a second rate zonal centrifugation, to micelles of quil a glycoside, already added to the gradient. haemagglutinating peaks were collected and us ... | 1987 | 2823496 |
| pasteurella haemolytica serotype 1 colonization of the nasal passages of virus-infected calves. | nasal passages of calves with a virus-induced respiratory tract disease became colonized by pasteurella haemolytica serotype 1 after they were inoculated intranasally with p haemolytica. inoculation with infectious bovine rhinotracheitis virus caused a more severe clinical illness and resulted in a greater degree of colonization with p haemolytica than developed after inoculation with parainfluenza-3 virus. nasal passages of parainfluenza-3 virus-inoculated calves were colonized to a greater deg ... | 1987 | 2829666 |
| the inhibition of bovine herpesvirus-1 by methyl 2-pyridyl ketone thiosemicarbazone and its effects on bovine cells. | methyl 2-pyridyl ketone thiosemicarbazone (mpkt) was found to inhibit bovine herpesvirus-1 (bhv-1) at an ed50 concentration of approximately 5-10 microm. several virus strains were shown to have similar sensitivity to the drug and serial passage of virus in the presence of mpkt failed to yield resistant progeny. there was evidence for toxic effects on cells at drug concentrations similar to those required to inhibit virus and passage of cells in low concentrations of mpkt gave results suggesting ... | 1987 | 2821894 |
| isolation of infectious bovine rhinotracheitis virus from a latently infected feral pig. | infectious bovine rhinotracheitis (ibr) virus was isolated from the trigeminal ganglion of a feral pig after dexamethasone treatment. three pigs inoculated intranasally with the ibr virus did not respond clinically or serologically. the virus was re-isolated from tonsillar swabs from two animals on post-infection day (pid) 3. | 1987 | 2821673 |
| specificity of the indirect enzyme-linked immunosorbent assay for detection of pseudorabies virus antibodies in pigs exposed to bovine herpesvirus-1. | six 5-week-old pigs were inoculated intranasally (in) with 10(7.6) tcid50 of bovine herpesvirus-1 (bhv-1). three of the pigs also were inoculated iv with a similar dose of bhv-1. clinical responses were not observed in these 6 pigs before oronasal challenge exposure with 10(7.8) tcid50 of virulent pseudorabies virus (prv) at postinoculation day 42. two pigs inoculated in with bhv-1 and challenge exposed with prv remained healthy, whereas the remaining 4 pigs developed severe clinical signs of ps ... | 1987 | 2823651 |
| mapping bovine herpesvirus type 1 latency-related rna in trigeminal ganglia of latently infected rabbits. | here we have used the bovine herpesvirus type 1 (bhv-1) rabbit model together with in situ nucleic acid hybridization to identify and map viral rna present in latently infected neurons. radioactively labeled cloned hindiii fragments representing most of the bhv-1 genome (cooper strain) were individually hybridized to sections of trigeminal ganglia taken from rabbits during acute and latent stages of infection. whereas all viral genomic fragments hybridized to lytically infected tissue culture ce ... | 1987 | 2824817 |
| map location of the thymidine kinase gene of bovine herpesvirus 1. | bovine herpesvirus 1 has been reported to contain a thymidine kinase (tk) gene which is nonessential for virus replication. we have isolated a thymidine kinase-negative mutant of the virus and localized the mutation by marker rescue experiments to a 1.1-kilobase bglii-sali fragment which maps at 0.47 to 0.48 on the bovine herpesvirus 1 genomic map. a thymidine kinase-negative bovine cell line isolated in our laboratory was used in these studies. | 1987 | 2824831 |
| rapid, sensitive, competitive serologic enzyme-linked immunosorbent assay for detecting serum antibodies to bovine herpesvirus type 1. | an enzyme-linked immunosorbent assay (elisa) for the detection of cattle antibodies to bovine herpesvirus type 1 was developed on the basis of competition between serum antibody and a virus-neutralizing mouse monoclonal antibody. the assay showed improved sensitivity over the virus neutralization (vn) test and over an enhanced vn test in which incubation of antibody-virus mixtures was carried out for 24 h. with the elisa, antibodies in sera from experimentally infected cattle were detected earli ... | 1987 | 2828419 |
| generation of il-2 dependent bovine cytotoxic t lymphocyte clones reactive against bhv-1 infected target cells: loss of genetic restriction and virus specificity. | to gain insight into the mechanisms of immunity to bovine herpesvirus type-1 (bhv-1) in particular the importance of the t-cell response, we attempted to clone bovine cytotoxic t lymphocytes that were specific for bhv-1 infected autologous target cells. a number of bovine t cell clones were generated by limiting dilution in the presence of bovine recombinant il-2 and bhv-1 infected target cells as feeder layers. these clones were maintained in culture on crude il-2 containing supernatants. in fu ... | 1987 | 2855701 |
| protection of newborn calves against fatal multisystemic infectious bovine rhinotracheitis by feeding colostrum from vaccinated cows. | to determine whether consumption of colostrum with high levels of serum neutralizing antibody to bovine herpesvirus 1 would protect neonatal calves from the frequently fatal multisystemic form of infectious bovine rhinotracheitis, holstein calves were fed for 48 h after birth with either pooled colostrum from seropositive vaccinated cows or colostrum from seronegative unvaccinated cows. the serum neutralizing antibody achieved in the former calves was between 64 and 256 and the titer in the latt ... | 1987 | 2839276 |
| [cellular and humoral immune responses in calves after experimental infection with bovine herpesvirus 1]. | studied were some of the humoral and cellular factors of immunity with calves experimentally infected with herpes virus 1. the clinical signs manifested by the infected calves were followed up for 10 to 12 days. the shedding of the virus through the nasal mucosa was demonstrated up to the eighth day, the peak values reached being between the 3rd and 5th day of infection. demonstrated was blastic transformation in vitro with a phytohemagglutinin of the lymphocytes obtained from the peripheral blo ... | 1987 | 2830708 |
| bovine herpesvirus type 1 gp87 mediates both attachment of virions to susceptible cells and hemagglutination. | the 87,000-dalton glycoprotein (gp87) of bovine herpesvirus type 1 (bhv-1) was found to selectively attach to susceptible cells. the attachment of gp87 to the cells was markedly decreased by the prior adsorption of intact virions. anti-gp87 (site ia) monoclonal antibody, which inhibited bhv-1 adsorption to the cells and neutralized the virus without complement [okazaki et al., virology 250: 260-264], was effective in inhibiting the adsorption of gp87. only the same antibody was able to inhibit t ... | 1987 | 2827610 |
| antibodies to bovine bacterial and viral pathogens in pronghorns in alberta, 1983. | sera from 210 pronghorns (antilocapra americana) ranging in southeastern alberta were tested for antibodies to disease agents present in indigenous cattle. no antibodies to brucella abortus, leptospira interrogans serovars pomona, hardjo, or grippotyphosa, or infectious bovine rhinotracheitis virus were found. antibodies at prevalences of 43.8% and 49.2% were detected to bovine virus diarrhea (bvd) and parainfluenza type 3 (pi-3) viruses, respectively. the much higher prevalence of bvd virus ant ... | 1986 | 2845155 |
| mechanisms of neutralization by monoclonal antibodies to different antigenic sites on the bovine herpesvirus type 1 glycoproteins. | monoclonal antibodies directed to different antigenic sites on bovine herpesvirus type 1 (bhv-1) glycoproteins were used to study the mechanisms of neutralization of the virus. three nonoverlapping neutralizing antigenic sites, designated ia, ib, and ic, were defined on gp87. antibodies to site ia which mediated viral neutralization without complement were effective on inhibition of virus adsorption. antibodies to a single neutralization site on gp71, designated iia, were able to neutralize the ... | 1986 | 2420063 |
| interferon production and replication of infectious bovine rhinotracheitis virus in fasted calves. | three groups of calves were inoculated intranasally with infectious bovine rhinotracheitis (ibr) virus, to determine any effects of a 3-day fast on virus replication and interferon (ifn) production measured in nasal secretions (ns). one group ("fasted") was inoculated 24 h after onset of the fast and another ("refed") at the end of fasting, immediately before refeeding. a third ("control") group was inoculated but not fasted. in fasted calves, overall mean virus excretion (during the first 5 pos ... | 1986 | 2425016 |
| a proof of protection of cattle with differing degrees of immunity against infectious bovine rhinotracheitis against experimental infection by aujeszky's disease virus. | 1986 | 2431562 | |
| experimentally induced infectious bovine rhinotracheitis virus infection during early pregnancy: effect on the bovine corpus luteum and conceptus. | pairs of heifers were inoculated iv with infectious bovine rhinotracheitis virus on postbreeding days (pbd) 7, 14, 21, or 28, and were euthanatized 13 to 15 days after inoculation. reproductive tracts were examined for cytopathologic changes (light microscopy), virus (cell culture), and viral antigen (immunohistochemical evaluation). heifers inoculated on pbd 7 or 14 had mild oophoritis characterized by foci of necrosis and mononuclear cell accumulations in the corpus luteum. most of these heife ... | 1986 | 2420240 |
| bovine t lymphocyte response to bovine herpesvirus-1: cell phenotypes, viral recognition and acid-labile interferon production. | the phenotype of bovine cells that proliferate to bovine herpesvirus (bhv-1) were identified by peanut agglutinin (pna) and monoclonal antibodies with specificity for pan t cells (b29), a t cell subset (b24), and an mhc class ii (ia-like) antigen (r-1). pna+ cells but not pna- cells separated by fluorescent activated cell sorting responded to bhv-1. virally-activated t cells expressed mhc class ii antigens, and possibly antigen specific receptors for virus. binding of radiolabeled virus increase ... | 1986 | 2421478 |
| synthesis and processing of bovine herpesvirus 1 glycoproteins. | four unique glycoproteins or glycoprotein complexes were recognized by a panel of monoclonal antibodies to bovine herpesvirus 1 (bhv-1), i.e., gvp 6/11a/16 (130,000-molecular-weight glycoprotein [130k glycoprotein]/74k/55k), gvp 7 (108k), gvp 3/9 (180k/91k), and gvp 11b (71k). the absence of any antigenic or structural relationship between gvp 11a and gvp 11b, which were previously identified as one glycoprotein, gvp 11, demonstrated that these two gvp 11 species are unique glycoproteins. gvp 3 ... | 1986 | 2426466 |
| bovine herpesvirus 1: strain comparison of polypeptides and identification of a neutralization epitope on the 90-kilodalton hemagglutinin. | the intracellular and structural polypeptides of the los angeles and cooper 1 reference strains of bovine herpesvirus 1, together with 12 other canadian field isolates, were analyzed by polyacrylamide gel electrophoresis. although a few minor differences were noted among some isolates in regard to intracellular viral protein content, analysis of partly purified virus showed strikingly similar polypeptide profiles among 19 proteins with molecular masses of 14 to 145 kilodaltons (kda). moreover, a ... | 1986 | 2427748 |
| polypeptide specificity of the antibody response after primary and recurrent infection with bovine herpesvirus 1. | the polypeptide specificities and defense mechanisms of the humoral immune response to bovine herpesvirus 1 were analyzed. sequential serum samples taken from cows which were experimentally infected with bovine herpesvirus 1 were tested for their reactivity with individual bovine herpesvirus 1 polypeptides by immunoprecipitation, immunoblotting, and enzyme-linked immunosorbent assay. all bovine immune sera reacted with each of the three major bovine herpesvirus 1 glycoproteins, gvp 6/11a/16, gvp ... | 1986 | 3009533 |
| logical description of bovine herpesvirus type 1 latent infection. | description of the interactions between bovine herpesvirus type 1 (bhv-1) and cattle was performed by the method known as kinetic logic. this logical formalization uses variables with two possible values, 1 and 0, which tell whether an element is present or not at a significant level. to each variable is associated a function which tells if the element is being produced at a significant rate. the temporal relation between a variable and its associated function is given by specific time delays. t ... | 1986 | 3009689 |
| [comparison of the immunizing effectiveness of subunit vaccine and inactivated viral oil vaccine against infectious rhinotracheitis of cattle]. | trials were conducted on rabbits and cattle to compare the immunizing effectiveness of the subunit vaccine against infectious bovine rhinotracheitis (ibr), representing antigens separated by the solubilization of the ibr virus-infected cells by means of triton x-100 with oil adjuvant, with the inactivated oil ibr vaccine. the rabbits inoculated and re-vaccinated with both vaccines in an interval of three weeks produced neutralizing antibodies in medium titres, the values of these antibodies were ... | 1986 | 3010533 |
| development of specific antibodies mediating antibody-dependent cell-mediated cytotoxicity following experimental infection of cattle with bovine herpesvirus 1 and subsequent viral reactivation. | evolution of sensitizing antibodies involved in antibody-dependent cell-mediated cytotoxicity (adcc), antibody-complement cell lysis and complement-facilitated adcc was followed in bulls after primary infection by a wild strain of infectious bovine rhinotracheitis virus (bovine herpesvirus 1; bhv-1) and after experimental reactivation of the virus. these antibodies were detected between the 4th and the 7th day after primary infection, reached a maximum level after 2 weeks and rose slightly after ... | 1986 | 3010549 |
| in vitro induction of cytotoxic lymphocytes from infectious bovine rhinotracheitis virus hyperimmune cattle. | cytotoxic lymphocytes against infectious bovine rhinotracheitis virus (ibrv)-infected cells were induced by in vitro stimulation with ibrv antigen of peripheral blood leukocytes obtained from hyperimmune cattle. after one in vitro stimulation with ibrv, cytotoxicity was detected against ibrv-infected autologous target cells, but not against heterologous ibrv-infected or k562 target cells. after 4 in vitro ibrv stimulations at weekly intervals (in the presence of autologous feeder cells followed ... | 1986 | 3024535 |
| bovine herpesvirus-1 and pasteurella haemolytica aerobiology in experimentally infected calves. | eight calves (2 calves in each of 4 groups) were exposed to an aerosol of bovine herpesvirus-1 (bhv-1) and 4 days later to an aerosol of pasteurella haemolytica. samples of tracheal and exhaled air were taken simultaneously beginning 1 day before viral exposure and once a day up to 3 to 4 days after the bacterial exposure. samples were also taken during the period of aerosol exposure. only 0.04% to 0.42% of p haemolytica-carrying droplets of the bacterial aerosol passed beyond the cranial part o ... | 1986 | 3006556 |
| intramuscular and intravaginal vaccination of pregnant cows with thymidine kinase-negative, temperature-resistant infectious bovine rhinotracheitis virus (bovine herpes virus 1). | to test the safety and efficacy of a thymidine kinase-negative (tk-), temperature-resistant (tr) mutant of bovine herpes virus-1 (bhv-1) in pregnant cows, seronegative cows, 2-5 months pregnant, were vaccinated intramuscularly (i.m.) or intravaginally (i.vag.) with this candidate vaccine virus. i.m. vaccinated cows did not shed virus i.vag. or intranasally (i.n.), but i.vag. vaccinated cows replicated virus i.vag. for 8-9 days postvaccination (p.v.) some of the cows were challenge exposed i.n. a ... | 1986 | 3008461 |
| patterns of bovine t cell-mediated immune responses to bovine herpesvirus 1. | lymphocyte proliferation was used to evaluate t cell-mediated immune responses to different isolates of bovine herpesvirus 1 (bhv-1). groups of high, moderate, and low responses were observed when lymphocytes from three breeds of dairy cattle were stimulated with each of the bhv-1 isolates. proliferation of cells in the low responding group could be augmented by exogenous il-2. the mechanism of unresponsiveness by cells from one individual whose response was not altered by il-2 supplementation w ... | 1986 | 3029942 |
| alteration of alveolar macrophage functions after aerosol infection with bovine herpesvirus type 1. | calves were aerosol challenged with bovine herpesvirus type i, and bronchoalveolar cells were subsequently retrieved by lavage from days 1 to 8 postinfection. alveolar macrophages (am), which were depleted of contaminating cells, were characterized with respect to phenotypic markers and functional activities. in most aspects, the changes suggested a stimulation of the am. with variations in kinetics the percentage of am expressing an mhc ii antigen and fc (immunoglobulin g)-mediated phagocytosis ... | 1986 | 3000945 |
| detection of impaired t cell-mediated immune responses to herpesvirus (bhv-1) in cattle. | interleukin 2 (il-2) functions in the regulation of cell-mediated immune responses both in vivo and in vitro. therefore, il-2 production has been studied in patients with immunological disorders to determine the level of the immune defect. however, the cause(s) of low responses to selected antigens by cells from clinically normal individuals has not been examined. the ability of cells from clinically normal individual cattle to produce and respond to il-2 was investigated as a measure of specifi ... | 1986 | 3020771 |
| cell-mediated cytotoxicity of peripheral blood mononuclear cells stimulated in vitro for infectious bovine rhinotracheitis virus-infected cells. | peripheral blood mononuclear cells (pbmc) from calves infected with and hyperimmunized to infectious bovine rhinotracheitis virus (ibrv) were stimulated in vitro with viral antigens to evaluate their cytotoxicity for a variety of cells. the 51-cr release assay was used to measure cytotoxicity. cytotoxicity was not present in fresh nonstimulated cells, but was detected in cultured, ibrv-stimulated cells at day 3, was maximal at day 7, and declined thereafter. pbmc stimulated in vitro with ibrv ex ... | 1986 | 3026081 |
| bovine herpesvirus 1: molecular and antigenic characteristics of variant viruses isolated from calves with neurological disease. | this report presents data showing that several virus isolates recovered in argentina, mainly from calves with non-purulent meningo-encephalitis, represent a hitherto unrecognized antigenic variant of bhv-1. the following experimental approaches have been adopted to demonstrate both the unique features among and the relatedness with bhv-1 of these isolates: i) crossed serum neutralization test with rabbit immune sera, ii) analysis by sds-polyacrylamide gel electrophoresis of radio-labeled virus i ... | 1986 | 3004386 |
| characterization of envelope proteins of infectious bovine rhinotracheitis virus (bovine herpesvirus 1) by biochemical and immunological methods. | ten glycoproteins of molecular weights of 180,000, 150,000, 130,000, 115,000, 97,000, 77,000, 74,000, 64,000, 55,000, and 45,000 (designated as 180k, 150k, etc.) and a single nonglycosylated 107,000-molecular-weight (107k) protein were quantitatively removed from purified bovine herpesvirus 1 (bhv-1) virions by detergent treatment. immunoprecipitations with monospecific and monoclonal antibodies showed that three sets of coprecipitating glycoproteins, 180k/97k, 150k/77k, and 130k/74k/55k, were t ... | 1986 | 3005625 |
| short repeats cause heterogeneity at genomic terminus of bovine herpesvirus 1. | analysis of the genomes of different bovine herpesvirus 1 strains revealed a ul terminal hindiii fragment differing in size (from 2.4 to 2.8 kilobases). this fragment polymorphism occurred in the dna of a wild-type isolate, in highly passaged, apathogenic tissue culture derivatives, and in plaque-purified substrains. this heterogeneity was due to variations in the copy number of a 14-base-pair tandem repeat comprising the base sequence 5'-gctcctcctccctc-3', which also exists, with some differenc ... | 1986 | 3005657 |
| neutralizing monoclonal antibodies directed to infectious bovine rhinotracheitis virus. | infectious bovine rhinotracheitis virus (ibrv) has been shown in this report to have thirty-three polypeptides. ten of the eleven polypeptides which can be labeled with (3h)-glucosamine are located on the surface of the virus since they can be surface labeled with sodium boro(3h)hydride. in order to define the immunologically important viral proteins, monoclonal antibodies were prepared against the virus and selected for their ability to neutralize infectivity. four such hybridoma lines were obt ... | 1986 | 3010905 |
| nitrogen metabolism of calves inoculated with bovine adenovirus-3 or with infectious bovine rhinotracheitis virus. | beef calves were inoculated with bovine adenovirus-3 or infectious bovine rhinotracheitis virus. after inoculation, plasma fibrinogen increased, serum phosphorus decreased, and nitrogen and phosphorus digestibility decreased compared with preinoculation values. urinary n excretion increased when calves developed rectal temperatures greater than 39.7 c. results indicated that clinical infection of calves with infectious bovine rhinotracheitis virus increases urinary n excretion and reduces n and ... | 1986 | 3013053 |
| influence of induced disease states on the disposition kinetics of imidocarb in goats. | the influence of fever, induced by different agents, on the disposition kinetics of imidocarb was determined in goats. escherichia coli endotoxin (0.2 microgram/kg), trypanosoma evansi (10(7) in 1 ml sterile glucose citrate), and infectious bovine rhinotracheitis virus (10(6.5)tcid50) were the agents administered to induce the febrile state. in control and febrile animals the two-compartment model was used to describe the disposition kinetics of the drug. fever caused significant changes to occu ... | 1986 | 3014166 |
| identification of bovine herpesvirus-1 polypeptides involved in serum neutralization. | bovine herpesvirus (infectious bovine rhinotracheitis virus)-infected cell antigens were solubilized with nonidet p-40. the crude antigen extract was separated by reaction with bovine hyperimmune serum in line immunoelectrophoresis; individual immunoprecipitates were used to immunize rabbits. rabbit sera possessing serum neutralizing activity were analyzed by reaction with crude antigen extract in immunoprecipitation sodium dodecylsulfate polyacrylamide gel electrophoresis (sds-page) and western ... | 1986 | 3016973 |
| evidence for an immunocompromising effect of bovine pestivirus on bovid herpesvirus 1 vaccination. | five calves were given live intranasal vaccine against bovid herpesvirus 1 (bhv1) two days after intranasal inoculation of bovine pestivirus (bvdv). another 5 were vaccinated in the absence of bvdv. control unvaccinated groups were also maintained. all calves were challenged with virulent bhv1. the unvaccinated calves developed signs of infectious bovine rhinotracheitis (ibr) and both vaccinated groups showed a similar degree of clinical protection from ibr. those given bvdv before vaccination s ... | 1986 | 3016976 |
| investigation of possible vaccine-induced epizootics of infectious bovine rhinotracheitis, using restriction endonuclease analysis of viral dna. | viral dna was extracted from each of 14 modified-live (ml) bovine herpesvirus 1 vaccines, representing all of the ml infectious bovine rhinotracheitis virus (ibrv) vaccines licensed by the us department of agriculture for use in cattle. restriction endonucleases pst i and bgl ii were used to establish restriction enzyme patterns for the vaccinal viruses. viral dna from isolates obtained from 6 field samples of ibrv (1 from colorado, 1 from west virginia, 3 from wisconsin, 1 from south dakota) we ... | 1986 | 3019192 |
| [ibr/ipv serodiagnosis with elisa methods in blood, single milk and bulk milk samples; control measures for maintaining normal cattle herds; eradication measures with reference to vaccination]. | 1986 | 3019627 | |
| detection of bovine herpesvirus type 1 rna in trigeminal ganglia of latently infected rabbits by in situ hybridization. | at times after conjunctival inoculation with bovine herpesvirus type 1 (bhv-1), representing the acute and latent phases of infection, rabbit trigeminal ganglia were examined for the presence of bhv-1 nucleic acids by in situ hybridization using a 3h-labelled bhv-1 dna probe. during the acute phase of virus infection, both bhv-1 dna and rna were detected in ganglionic neurons and occasionally in adjacent satellite cells. however, during the latent phase of infection only viral rna was detectable ... | 1986 | 3023537 |
| reduction in in vivo testing at the national veterinary services laboratories of the united states department of agriculture. | efforts are being made at the national veterinary services laboratories to reduce in vivo testing of usda licensed veterinary vaccines. a hemagglutination test for determining potency of killed parvovirus vaccine is currently being used for canine and swine adjuvanted and nonadjuvanted products; a serum neutralization inhibition test (snit) is being developed for potency testing of killed adjuvanted infectious bovine rhinotracheitis (ibr), bovine virus diarrhea (bvd) and parainfluenza (pi3) vacc ... | 1986 | 3025035 |
| map location of the gene for a 130,000-dalton glycoprotein of bovine herpesvirus 1. | a bovine herpesvirus 1 variant (mar6) containing a mutation in a viral glycoprotein with a molecular weight of 130,000 (g130) was isolated by selecting for resistance to a neutralizing monoclonal antibody (130-6) directed against g130. mar6 was completely resistant to neutralization by monoclonal antibody 130-6 in the presence and absence of complement, but was neutralized by polyvalent immune sera. the mar6 mutant synthesized and processed g130, but produced plaques which failed to react with m ... | 1986 | 3021976 |
| [prevalence of bovine herpesvirus 4 in the swiss cattle population and possible serologic cross reaction with bovine herpesvirus 1 (ibr/ipv virus)]. | 1986 | 3022374 | |
| induction of immunity against pneumonic pasteurellosis following experimental infection in calves. | immunity against pneumonic pasteurellosis was studied in calves after recovery from experimental respiratory disease with pasteurella haemolytica. nine calves were exposed to aerosols of parainfluenza-3 virus and pasteurella haemolytica a1 six days apart to produce respiratory disease. after recovery from the disease, these nine principal and four control calves were challenged with aerosols of bovine herpesvirus 1 and p. haemolytica a1 four days apart. with this viral-bacterial challenge, the n ... | 1986 | 3017526 |
| immunologic comparison of the proteins of pseudorabies (aujeszky's disease) virus and bovine herpesvirus-1. | the immunologic relationship between bovine herpesvirus-1 and pseudorabies virus was examined by 80% serum cross-neutralization test, enzyme-linked immunosorbent assays, and western immunoblotting procedures. immunogenic cross reactivity between the 2 viruses was observed with both the serum-neutralization test and the enzyme-linked immunosorbent assay. a probing of viral western immunoblots with rabbit hyperimmune antiserum showed that there were a number of viral-specific cross-reactive protei ... | 1986 | 3019188 |
| identification of the thymidine kinase gene of infectious bovine rhinotracheitis virus and its function in escherichia coli hosts. | in an attempt to understand the gene expression of the infectious bovine rhinotracheitis virus (ibrv), the viral thymidine kinase gene (tk), a well regulated viral gene has been chosen for this study. a cosmid library of ibrv has been constructed in escherichia coli hb101 by cloning partially sau3a-digested dna fragments into a cosmid vector, pjb8. recombinant cosmids were further analyzed by restriction digestions and by southern blot hybridization. results showed that this cosmid library compr ... | 1986 | 3023190 |
| the genome of bovine herpesvirus 1 (bhv-1) strains exhibiting a neuropathogenic potential compared to known bhv-1 strains by restriction site mapping and cross-hybridization. | bovine herpesvirus 1 (bhv-1) strains can be differentiated by their dna and polypeptide patterns, and by antigenic properties as demonstrated by monoclonal antibodies. we classified the bhv-1 strains according to these data as bhv-1.1, bhv-1.2 (a/b) and bhv-1.3 (a/b). bhv-1.1 and bhv-1.2 correspond to the well known 'common' bhv-1 strains, whereas bhv-1.3 has only recently been recognized and exhibits a neuropathogenic potential. in the present paper we describe the structural genome characteris ... | 1986 | 3026111 |
| an evaluation of five serological tests for the detection of antibody to bovine herpesvirus 1 in vaccinated and experimentally infected cattle. | more than 300 bovine sera from a previously reported vaccination and challenge trial were tested for antibodies to bovine herpesvirus 1 (bhv1) by five serological assays: enzyme-linked immunosorbent assay (elisa) for igm and igg, passive haemagglutination (pha), and two methods of virus neutralisation (vn). in a statistical comparison of elisa (igg), pha and vn results, the assays showed highly significant correlations (p less than 0.01). the sensitivities of elisa and 24-hour neutralisation tes ... | 1986 | 3027800 |
| restriction endonuclease patterns of bovine herpesvirus type 1 isolated from bovine mammary glands. | restriction endonuclease analysis was used, in conjunction with viral neutralization and growth-curve experiments, to compare a bovine herpesvirus type 1 (bhv-1) isolate, originally obtained from bovine mammary gland lesions, with a standard bhv-1 strain, infectious bovine rhinotracheitis virus. although differences were not detected by viral neutralization or growth-curve experiments, restriction fragment patterns generated by bam hi, eco ri, hind iii, and hpa i, revealed definite differences b ... | 1986 | 3026212 |
| enzyme linked immunosorbent assay used to monitor serum antibodies to bovine respiratory disease viruses. | an enzyme linked immunosorbent assay (elisa) was applied to the detection of serum antibodies against infectious bovine rhinotracheitis (ibr), parainfluenza-3 (pi3), adenovirus type 3 (adeno 3) and bovine respiratory syncytial (brs) viruses. paired serum samples from calves vaccinated with live attenuated virus vaccines were tested. the elisa compared favorably with the virus neutralization test for detecting serologic responses to ibr, brs, and adeno 3 viruses or with the hemagglutination inhib ... | 1986 | 3014711 |
| the association between serological titers in infectious bovine rhinotracheitis virus, bovine virus diarrhea virus, parainfluenza-3 virus, respiratory syncytial virus and treatment for respiratory disease in ontario feedlot calves. | a seroepidemiological study of the association between antibody titers to infectious bovine rhinotracheitis, parainfluenza-3, bovine virus diarrhea and bovine respiratory syncytial viruses, and treatment for bovine respiratory disease was conducted. a total of 322 calves from five different groups were bled on arrival, then one month later all cases (cattle treated for bovine respiratory disease) were rebled together with an equal number of controls (cattle not treated for any disease). titers t ... | 1986 | 3017529 |
| detection of bovine herpesvirus-specific nucleic acids by in situ hybridization with biotinylated dna probes. | biotin-labeled dna probes for bovine herpesvirus type 1 (bhv-1) were used to detect viral nucleic acids in infected cell cultures and clinical specimens by in situ hybridization. hybridization signal was detected 2 hours after inoculation in the cytoplasm of infected cells, presumably representing input virus. hybridization was first detected in the nucleus at 4 hours after inoculation; by 10 hours after inoculation, hybridization signal was detected in both nucleus and cytoplasm of almost 50% o ... | 1986 | 3008605 |
| effect of recombinant dna-derived bovine and human interferons on replication of bovine herpesvirus-1, parainfluenza-3, and respiratory syncytial viruses. | antiviral effects of recombinant dna-derived bovine (bo) and human (hu) interferons (ifn) on the replication of bovine herpesvirus-1, parainfluenza-3, and respiratory syncytial viruses were studied. bovine monolayer cultures were treated with recombinant dna-produced bo ifn-alpha 1, bo ifn-beta 2, hu ifn-alpha a, or hu ifn-alpha a/d and then challenge exposed with bovine herpesvirus-1, bovine parainfluenza-3 virus, bovine respiratory syncytial virus, or vesicular stomatitis virus. treatment with ... | 1986 | 3008606 |
| propagation of infectious bovine rhinotracheitis (bovine herpes-1) virus in murine primary cell cultures. | virus synthesis in balb/c mouse lung and kidney primary cultures infected with infectious bovine rhinotracheitis (ibr) virus started between 6 and 8 hours after virus inoculation and reached a maximum titer of 5.5 log10 plaque forming units (pfu) at 48 hours postinfection (pi). cytopathic effect (cpe) in cell cultures occurred at 8-10 hours and over 90% of the cells had cpe by 48 to 72 hours pi. the bulk of the newly replicated virus (60-80%) was cell-associated as determined by plaque assay of ... | 1986 | 3004011 |
| occurrence of antibodies to the etiologic agents of infectious bovine rhinotracheitis, parainfluenza 3, leptospirosis, and brucellosis in white-tailed deer in minnesota. | a serologic survey was conducted on 628 white-tailed deer (odocoileus virginianus) in 1976 and 1979-1980. tests for antibodies to the etiologic agents of infectious bovine rhinotrancheitis (ibr), parainfluenza 3 (pi3), leptospirosis, and brucellosis produced positive results of 15%, 20%, 3% and 0%, respectively. adult deer had significantly higher prevalence of antibodies to ibr virus and pi3 virus than fawns. these data provide a basis for monitoring these disease agents in minnesota's white-ta ... | 1986 | 3005666 |
| study on the etiologic role of bovine respiratory syncytial virus in pneumonia of dairy calves. | bovine respiratory syncytial virus (brsv) was the viral agent most commonly identified in 14 epizootics of pneumonia in dairy calves. a microtiter serum-virus neutralization test proved to be the best means of identifying involvement of brsv; seroconversion (fourfold or greater rise in titer) was demonstrated in 10 of the 14 epizootics. only limited involvement of bovine viral diarrhea virus, infectious bovine rhinotracheitis virus, parainfluenza 3 virus, and bovine adenovirus type 3 was recogni ... | 1986 | 3733504 |
| diagnosis of bovine respiratory syncytial virus infections improved by virus detection in lung lavage samples. | the potential of lung lavage to diagnose and study infections with bovine respiratory syncytial virus (brsv) was investigated. the technique was simple to perform under field conditions, and even in calves with respiratory tract disease, serious drawbacks were not observed. in 9 epizootics of respiratory tract disease, brsv infection was diagnosed in 1 or more calves. the brsv was detected from a total of 21 of 32 calves by immunofluorescence in cells recovered from lavage fluids, and the virus ... | 1986 | 3511803 |
| cytotoxicity of bovine lymphocytes after treatment with lymphokines. | cytotoxic lymphocytes were generated from bovine peripheral blood mononuclear leukocytes after in vitro stimulation with lymphokines that contained interleukin-2. lymphokine-stimulated cultures were cytotoxic to k562 cells (human natural killer [nk] targets) and yac-1 cells (mouse nk targets), but not to hsb-2 cells (human nk targets) in a 4-hour, 51cr-release assay. cells generated after lymphokine activation also mediated antibody-dependent cellular cytotoxicity to hsb-2 cells. appearance of e ... | 1986 | 3488701 |
| [bvd (bovine virus diarrhea) and ibr (infectious bovine rhinotracheitis): serologic studies on 35 farms]. | 1099 female cattle from 35 farms in the provinces of north brabant, gelderland and overijssel were examined for the presence of antibodies against bovine virus diarrhoea (bvd) and infectious bovine rhinotracheitis (ibr) by serum neutralisation tests. one of the farms examined was seronegative for bvd and 11 farms were seronegative for ibr. on the seropositive farms about 50% of cattle carrying their first calf had not experienced infection i.e. were seronegative (bvd 51.2%, ibr 48.3%). after cal ... | 1986 | 3027917 |
| colonization of the nasal passages of calves with pasteurella haemolytica serotype 1 and regeneration of colonization after experimentally induced viral infection of the respiratory tract. | healthy nonstressed calves were inoculated intranasally with or subjected to aerosol exposure to pasteurella haemolytica serotype 1. only 4 of 28 calves harbored the bacterium in enough numbers to be isolated from the nasal passages for more than 7 days. after apparent clearing from the nasal passages, 8 calves were inoculated intranasally with infectious bovine rhinotracheitis virus; 2 of these calves shed the p haemolytica during clinical illness due to the virus. the remaining 20 calves were ... | 1986 | 3752678 |
| evaluation of an enzyme-linked immunosorbent assay (elisa) for serodiagnosis of infectious bovine rhinotracheitis infection, with results of a preliminary survey. | an indirect enzyme linked immunosorbent assay (elisa) procedure was evaluated against the serum neutralisation test (snt) for the detection of antibodies to infectious bovine rhinotracheitis virus (bovine herpesvirus type l), using 2028 sera from 166 dairy and 172 beef cattle herds. the results showed the elisa to give high levels of agreement with the snt in classifying positive and negative sera (98% and 97% respectively). such disagreements as did occur involved weakly reactive sera with snt ... | 1986 | 16031249 |
| proceedings - embryo transfer in the canadian cattle industry status of disease transmission studies and thier relationship to the international movement of bovine embryos. | the current, generally accepted approach to formulating health requirements for the international movement of embryos is to base them on the health status of the male and female donor animals. the alternative approach of basing them on the health status of the embryos themselves has been blocked by the lack of scientific information about the potential of the early embryo to transmit agents of infectious disease. consequently, most research into infectious disease transmission by embryos has had ... | 1986 | 17422615 |
| eradication of infectious bovine rhinotracheitis virus (bovine herpesvirus 1) from a herd of beef cattle. | infectious bovine rhinotracheitis virus was eradicated from a 150 cow beef herd at the animal diseases research institute, lethbridge, alberta. tests used to accomplish this included standard and modified serum-virus neutralization tests and an enzymelinked immunosorbent assay. these results and those of preliminary pilot studies in the herd and in a nonvaccinated, infectious bovine rhinotracheitis-infected 450 cow beef herd suggest that eradication of infectious bovine rhinotracheitis infection ... | 1985 | 17422544 |
| prevalence of antibodies to specific infectious agents in bovine fetuses from a slaughterhouse in minnesota. | sera from 486 bovine fetuses, approximately 60 to 270 days of gestation, were collected at slaughter and tested for the presence of immunoglobulins (ig). one hundred ten (27%) of the sera were positive for igg and/or igm. the earliest age at which fetuses tested positive for igm and igg was estimated to be 100 and 120 days, respectively. ig concentration increased with increased age of the fetus. sera that were positive for ig were tested for the presence of specific antibodies to five different ... | 1985 | 16726037 |
| clinical and experimental modifications of plasma iron and zinc concentrations in cattle. | plasma iron and zinc concentrations were studied in pyrexic cattle or in cattle experimentally infected with infectious bovine rhinotracheitis virus or escherichia coli endotoxin. plasma iron and zinc levels tended to decline in the animals given endotoxin and in the pyrexic cattle, but the plasma iron level was only modified after experimental infectious bovine rhinotracheitis infection. these changes were not always related to pyrexia. plasma iron and zinc concentrations taken together may be ... | 1985 | 3890352 |
| preliminary studies with a live streptomycin-dependent pasteurella multocida and pasteurella haemolytica vaccine for the prevention of bovine pneumonic pasteurellosis. | twelve pasteurella-free holstein-friesian calves were used in a study to test the efficacy of a live streptomycin-dependent pasteurella multocida a:3 and streptomycin-dependent pasteurella haemolytica a1 vaccine. the calves were inoculated intramuscularly twice at 14-day intervals with either the streptomycin-dependent vaccine, containing 1 x 10(6) colony forming units/ml p. multocida and 4 x 10(8) colony forming units/ml p. haemolytica, commercial bacterin, or phosphate buffered saline. two wee ... | 1985 | 3907804 |
| morphometric studies of the bovine uterus: microscopic lesions and retrospective reproductive history. | a survey of reproductive tracts from nonpregnant cows at an abattoir was conducted. reproductive tracts from 98 cows were studied microscopically and evaluated with a grading system to determine the severity of pathologic changes. inflammatory reaction (type and distribution), fibrosis, gland morphology, and appearance were measured, quantified, and scored (1 to 4). category 1 endometrium (normal) was found in 18 cows, category 2 in 23, category 3 in 34, and category 4 in 23. the category of end ... | 1985 | 4083598 |