Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [survival of different strains of vibrio cholerae on fresh lettuce]. | temperature effect on the survival of vibrio cholerae in fresh lettuce was studied. remaining infectivity in leaves experimentally contaminated with different strains of vibrio cholerae was determined at 4 degrees c and 25 degrees c. the number of colonies in tcbs agar (vibrio cholerae) and in nutrient agar (vibrio cholerae and secondary microorganisms) was determined at to (initial time) and at ti (different post-contamination times). the ratio ti/to was calculated in both cases. different surv ... | 1996 | 8815457 |
| mucosal immune responses to intestinal bacterial pathogens. | current advances in the study of gut mucosal immunology and molecular biology have enhanced our ability to understand the pathogenesis of enteric bacterial infections as well as the role of the immune system in mediating both tissue injury and protection. in this article, we review the immunopathogenesis and the protective immune response to three enteric pathogens, vibrio cholerae, shigella, and salmonella. each of these pathogens has a distinctive mechanism by which it causes disease, ie, epit ... | 1996 | 8817767 |
| further studies on biochemical characteristics and serologic properties of the genus aeromonas. | we characterized a collection of 268 aeromonas isolates from diverse sources (clinical, animal, and environmental sources) for their species and serogroup designations. overall, 97% of these strains could be identified to the genomospecies level by using an expanded battery of biochemical tests. members of the aeromonas hydrophila complex (a. hydrophila, hg2, and a. salmonicida), a group that has previously been difficult to separate biochemically, could easily be distinguished from one another ... | 1996 | 8818884 |
| cloning and sequence of a region encoding a surface polysaccharide of vibrio cholerae o139 and characterization of the insertion site in the chromosome of vibrio cholerae o1. | vibrio cholerae serogroup o139 bengal is the first documented serogroup other than o1 to cause epidemic cholera. the o139 bengal strains are very similar to v. cholerae serogroup o1 biotype el tor strains. the major differences between the two serogroups are that o139 bengal contains a distinct o antigen and produces a polysaccharide capsule. we previously described three tnphoa mutants of o139 strain ai1837 which abolish both o antigen and capsule production. these tnphoa insertions were mapped ... | 1996 | 8820651 |
| [basic and clinical studies of pazufloxacin on infectious enteritis research group of t-3761 on infectious enteritis]. | a clinical study was carried out on pazufloxacin (pzfx) in 137 patients including shigellosis, salmonella enteritis, enteropathogenic esherichia coli enteritis and cholera, and carriers of these pathogens. antibacterial activity of pzfx against clinical isolates, fecal concentration of pzfx and effects of pzfx on fecal microflora were also investigated. the overall clinical efficacy rate was 97.2%. the bacteriological efficacy rates were 98.2% against shigella spp., 81.8% against salmonella spp. ... | 1996 | 8822054 |
| mutation and mutagenesis of thiol peroxidase of escherichia coli and a new type of thiol peroxidase family. | a novel thioredoxin-linked thiol peroxidase (px) from escherichia coli has been reported previously (m. k. cha, h. k. kim, and i. h. kim, j. biol. chem. 270:28635-28641, 1995). in an attempt to perform physiological and biochemical characterizations of the thiol px, a thiol px null (tpx) mutant and a functional-residue mutant of thiol px were produced. the tpx mutant was viable in aerobic culture but grew more slowly than the wild-type cells. the difference in growth rate became more pronounced ... | 1996 | 8824604 |
| the cdw65 monoclonal antibodies vim-8 and vim-11 bind to the neutral glycolipid v3fucnlc8cer. | at the ivth and vth workshop on human leukocyte differentiation antigens a group of monoclonal antibodies recognizing myeloid cells was found to bind to the ganglioside x3-neuacvii3fucnlc10cer (vim-2 dodecasaccharide). these antibodies were given the provisional cluster of differentiation designation cdw65. three antibodies of this cluster (vim-2, vim-8, and vim-11) have now been studied in detail at the molecular and the cellular level. binding of vim-2 is abolished after treatment of cells wit ... | 1996 | 8830039 |
| bacterial ghosts: non-living candidate vaccines. | expression of cloned phix174 gene e in bacteria results in lysis of bacteria. it is unique among phage lysis systems as it introduces a transmembrane tunnel structure through the cell envelope complex of gram-negative bacteria. the resulting bacterial ghosts have intact envelope structures devoid of cytoplasmic contents. e-mediated lysis has been achieved in a variety of gram-negative bacteria including escherichia coli, salmonella typhimurium, vibrio cholerae, klebsiella pneumoniae, and actinob ... | 1996 | 8717400 |
| expression of shigella sonnei lipopolysaccharide in vibrio cholerae. | making use of a newly designed mobilizable suicide vector, the genetic determinants encoding shigella sonnei lipopolysaccharide (lps) were stably integrated into the chromosome of the live attenuated vibrio cholerae vaccine strain cvd103-hgr. expression studies showed that the production of complete s. sonnei o-polysaccharide (o-ps)-bearing lps was limited in bivalent recombinant strains that were also proficient in the synthesis of the host-encoded inaba o-ps. conversely, high amounts of lps ca ... | 1996 | 8830276 |
| [isolation of vibrio cholerae in imported frozen seafood and their cholera-enterotoxin production]. | a survey study for vibrio cholerae in imported seafood was conducted during january 1991 to december 1994. a total of 7,439 specimens (approximately 20% of all imported food) were randomly picked up and examined for contamination of v. cholerae. among these, v. cholerae o1 were isolated from 9 specimens, but they were all cholerae enterotoxin (ct)-negative. in terms of v. cholerae non-o1, a total of 2,803 specimens (37.4%) were contaminated with this vibrio. shrimp, especially the ones still in ... | 1996 | 8851390 |
| non-o:1 vibrio cholerae bacteremia and peritonitis in a patient with nephrotic syndrome. | 1996 | 8852924 | |
| detection of viable vibrio cholerae by reverse-transcriptase polymerase chain reaction (rt-pcr). | the use of conventional pcr can amplify target dna from both viable and nonviable cells of vibrio cholera. detection of only viable microbial pathogens in biological samples, especially clinical and food samples, is usually desired to ensure positive test results are associated with active agents, and not the remains of dead cells. positive identifications caused by nonliving causative agents may lead to misguided decisions concerning the effectiveness of treatment, and whether patient treatment ... | 1996 | 8853011 |
| factors influencing the stability of live oral attenuated bacterial vaccines. | live oral attenuated vaccines against typhoid fever (salmonella typhi ty21a) and cholera (vibrio cholerae cvd 103-hgr) have been licensed for human use. vaccine potency is dependent upon each dose containing a minimum number of viable organisms and galenic parameters. to ensure long-term stability, such vaccines must be stored at 5 degrees c +/- 3 degrees c. however, exposure to ambient temperatures (25 degrees c) for short periods of time (< 7 days) does not compromise vaccine potency. brief ex ... | 1996 | 8854028 |
| genetic manipulation of vibrio cholerae for vaccine development: construction of live attenuated el tor candidate vaccine strains. | the recent spread of el tor cholera to america augments the need for an effective, safe and economical vaccine. in the present paper we describe the construction of live attenuated v. cholerae strains by specifically deleting the genes encoding cholera toxin and other putative toxins from the bacterial chromosome. to maximize the likelihood of exposing protective antigens relevant to currently circulating vibrios we selected for genetic manipulation recent epidemic v. cholerae isolates from peru ... | 1996 | 8854382 |
| oral rehydration therapy. | oral rehydration solution (ors), the best treatment of dehydration due to acute diarrhea, is the most important medical advance of this century since it is key to reducing infant and child morbidity and mortality. pathogens responsible for acute diarrhea include those which produce enterotoxin at the intestinal mucosal surface, inducing secretion but are not invasive (e.g., vibrio cholerae); those which invade and disrupt the mucosal lining (e.g., shigella species); and rotavirus. the world he ... | 1996 | 8855579 |
| vibrio cholerae 01 isolated in the gallbladder of a patient presenting with cholecystitis. | cholera is a topical infection of the intestinal tract and rarely causes extraintestinal disease. the gallbladder has been proposed to be the reservoir of this organism. we present a patient from bolivia who developed symptoms of acute cholecystitis and whose bile culture grew vibrio cholerae 01 el tor. | 1996 | 8855762 |
| hlya hemolysin of vibrio cholerae o1 biotype e1 tor. identification of the hemolytic complex and evidence for the formation of anion-selective ion-permeable channels. | hemolysin (hlya) was concentrated from supernatants of different vibrio cholerae o1 biotype e1 tor strains by ammonium sulfate precipitation. the concentration of the toxin in the supernatants and in the precipitates was quantified using its hemolytic activity. the toxin formed a high molecular-mass band (about 220 kda) on sds/page while the toxin monomer had a molecular mass of 60 kda when it was heated. the addition of the e1 tor hemolysin oligomers, but not that of the monomers, to the aqueou ... | 1996 | 8856066 |
| activation of bradykinin generating cascade by vibrio cholerae protease. | 1996 | 8856191 | |
| vibrio cholerae o1 meningitis in an immunosuppressed child. | 1996 | 8858687 | |
| genetic footprint on the toxr-binding site in the promoter for cholera toxin. | the transmembrane dna-binding protein, toxr, of vibrio cholerae is a global transcriptional regulator of virulence gene expression. toxr has been shown to interact with promoter regions upstream of both the ctxab operon encoding cholera toxin, and the regulatory gene toxt. deletion analysis has shown that a repeated sequence, ttttgat, is required for toxr binding and activation of the ctxab promoter. however, this sequence is not found upstream of the toxt promoter. genetic selections using p22 ... | 1996 | 8861218 |
| influence of the copepod mesocyclops longisetus (crustacea: cyclopidae) on the survival of vibrio cholerae o1 in fresh water. | in an experimental microcosm, an analysis was performed of the influence exerted by freshwater mesocyclops longisetus copepods on the survival of vibrio cholerae o1 serovar inaba. in the state of ceará, copepods are used in the control of aedes aegypti larvae. the system consisted of water with a salinity of 0.27 per 10,000 habitants and ph 7.5, which after sterilizing filtration was distributed into seven flasks with a volume of 400 ml; in each of six flasks, 10 live copepods were inoculated al ... | 1996 | 10904360 |
| cholera-brucella cross-reaction: a new potential diagnostic problem for travelers to latin america. | background: brucellosis is an endemic disease in latin america and other countries. serologic cross-reaction between cholera and brucella infection is well recognized. since the introduction to cholera in 1991 in latin america, interpreting serologic tests has become potentially problematic. this study attempts to evaluate this problem. methods: tube agglutination tests were performed to detect brucella antibodies in 44 peruvian adult patients with moderate to severe diarrhea due to vibrio chole ... | 1996 | 9815420 |
| survey on the distribution of vibrionaceae at the seaport areas in taiwan, 1991-1994. | a monthly survey on the distribution of human-pathogenic vibrionaceae of the seawater from five principal harbors in taiwan was conducted by national quarantine service from july, 1991 to february, 1994. of the total 1,167 vibrionaceae isolates, strains of vibrio alginolyticus (449 strains) were the most frequently isolated, followed by vibrio parahaemolyticus (262) , aeromonas hydrophila (153), vibrio cholerae non-o1 (86), and vibrio vulnificus (67). none of vibrio cholerae o1 was isolated. the ... | 1996 | 10592802 |
| [phenotypic and molecular features of vibrio cholerae isolated in chile, peru and bolivia. comparison with environmental reservoirs]. | since 1991, a massive cholera epidemic started in peru and involved most central and south american countries. in chile, 147 cases were registered, the last one in 1995. | 1996 | 9334476 |
| bacterial diarrhea in laos, a region where cholera was endemic. | there were cholera outbreaks in lao pdr since 1993. two provinces were epidemic areas of cholera in 1993. however the area of cholera occurrence increased to 7 provinces through 1994. then a bacterial survey had done for the purpose of public health improvement. epec was markedly isolated from the hospital in vientiane. in apparent infection of salmonella and non-01 vibrio cholerae with soldiers stationed in the island in mekong river belong to vientiane were pointed out. the outbreak of diarrhe ... | 1996 | 9253874 |
| a rapid method for restriction analysis of large plasmids from enteric pathogens. | a modified version of the method of kado and liu (j bacteriol 1981, 145: 1365) has been developed for rapid detection and direct cleavage analysis of large plasmids from vibrio cholerae and other enteric pathogens. | 1996 | 9257344 |
| erythromycin and trimethoprim-sulphamethoxazole in the treatment of cholera in children. | to evaluate the efficacy of erythromycin and trimethoprim-sulphamethoxazole (tmp-smx) in the treatment of cholera in children aged 1-8 years, a randomised clinical trial was conducted at a diarrhoea treatment centre in bangladesh from december 1991 to june 1992. fifteen children received erythromycin, 50 mg/kg per day, in four equally divided doses, 18 children received 10 mg/kg per day of trimethoprim and 50 mg/kg per day of sulphamethoxazole in two equally divided doses (12 hourly) for five da ... | 1996 | 9203786 |
| differentiation of vibrio cholerae o1 isolates with biochemical fingerprinting and comparison with ribotyping. | the phene plate (php) system is a commercially available typing system based on the measurements of kinetics of selected biochemical reactions of bacteria grown in liquid medium in 96-well microplates. the system uses numerical analysis to identify biochemical phenotypes among the tested strains. in the present study, a set of 16 discriminatory tests were used to differentiate 117 strains of vibrio cholerae o1 from mexico and bangladesh. the stability of php types of 16 isolates under different ... | 1996 | 9203787 |
| rapid detection of cholera toxin gene of vibrio cholerae o1 by polymerase chain reaction. | 1996 | 9206089 | |
| [presence and diffusion of vibrio cholerae in aquatic environment]. | 1996 | 9235016 | |
| characterisation of vibrio cholerae 0139 isolated from diarrhoeal stools. | a total of 174 samples of acute diarrhoeal stools received over a period of seven months, yielded 101 isolated morphologically and biochemically resembling vibro cholerae. serologically, 57(56.4 per cent) of the 101 isolates were identified as v. cholerae 01 and remaining 44 (43.6 per cent) as v. cholerae 0139. keeping in mind the unique potential of 0139 among non 01 vibrio to cause epidemics, we decided to undertake the study of biochemical characters and physiological behaviour of all the 44 ... | 1996 | 9401240 |
| non-0:1 vibrio cholerae septicemia in thalassemia patients. | 1996 | 9280014 | |
| [community trial for safety and immunogenicity of oral-administered lyophilized rbs-wc cholera vaccine]. | in order to evaluate the safety and immunogenicity of domestic produced lyophilized recombinant, b-subunit, inactivated whole cell vaccine of vibrio cholerae (rbs-wc), 369 subjects were randomly divided into three groups and observed with masking method, one with high dose of vaccine (5 mg rbs and 10" wc), another with low dose (1 mg rbs and 10" wc), and the control one with placebo. three doses of vaccine were given orally at an interval of seven days and 14 days, respectively. results showed t ... | 1996 | 9388905 |
| [the cellular fatty acid composition of bacteria in the family vibrionaceae]. | it is shown that strains of vibrio cholerae of serovar o1, biovar eltor, subtype ogawa, museum strains v. cholerae of serovar o1 and nag-vibrios (isolated from various sources: sea, river and sewage water, canal water and people) possess identical composition of cell fatty acids with prevailing hexadecenoic, hexadecanoic and octadecenoic acids. being identical, fatty acid profiles of v. parahaemolyticus and v. alginolyticus, are close to that of v. cholerae differing from the latter mainly by th ... | 1996 | 9044701 |
| detección de escherichia coli toxigénica (lt) mediante la reacción en cadena de la polimerasa [the detection of toxigenic escherichia coli (lt) by the polymerase chain reaction]. | in this paper it is described the detection enteroxigenic escherichia coli lt (+). this method is based on the amplification of a dna fragment of 400 pairs of bases by polymerase chain reaction (prc). the oligonucleotides were designed by the authors and the characteristic patterns were observed when the samples were submitted to an electrophoresis in an agarose gel at 2%. the pcr had positive results with the strains of escherichia coli 0:149 k; 88 (lt+) collection and with 20 strains isolated ... | 1996 | 9805043 |
| [the application of the hybridization in colonies technic for the identification of toxigenic vibrio cholerae 01]. | by means of the polymerase chain reaction (pcr) it was obtained a probe for the gen that codifies the subunit b of cholerae toxin (ctxb), which carried a vibrio cholerae 01 reference strain. the checking of the amplified product was performed by using the hybridization techniques in colonies. this product hybridized with the gen that codifies for the subunit b of cholerae toxin isolated from peru and ecuador, representing the present epidemics in latin america, but it did not so with the phyloge ... | 1996 | 9805044 |
| [cholera in a district of peru]. | taking to consideration the low report of cholera patients and with the main knowing the reality about the introduction of vibrio cholerae (v. cholerae) in peru, a sample of 101 cases with acute diarrheal disease (add) was taken at the distrito villa el salvador. they were selected by a systematic randomized sampling defined for each health care unit in the district, according to the daily average occurrence of add cases attended a week before the beginning of the study. all of them took part in ... | 1996 | 9805053 |
| septicemia caused by a non-01 vibrio cholerae. | 1996 | 17372457 | |
| cell harvesting by cross-flow microfiltration using a shear-enhanced module. | protein, produced by a bacterial culture of recombinant vibrio cholerae, was separated from cells in a fermentation broth by cross-flow microfiltration. a new, mechanically agitated (rotational) shear filter, the dmf(tm) filter from pall, was used to perform the separation. higher protein recovery and permeate flux than commonly obtained during cell harvesting were demonstrated using sixfold concentration followed by twofold diafiltration. the transmembrane pressure only increased by 10 kpa when ... | 1996 | 18629909 |
| medical microbiology | bacteria are classified and identified to distinguish one organism from another and to group similar organisms by criteria of interest to microbiologists or other scientists. the most important level of this type of classification is the species level. a species name should mean the same thing to everyone. within one species, strains and subgroups can differ by the disease they produce, their environmental habitat, a ... | 1996 | 21413329 |
| medical microbiology | vibrios are highly motile, gram-negative, curved or comma-shaped rods with a single polar flagellum. of the vibrios that are clinically significant to humans, vibrio cholerae o group 1, the agent of cholera, is the most important. vibrio cholerae was first isolated in pure culture by robert koch in 1883, although it had been seen by other investigators, including pacini, who is credited with describing it first i ... | 1996 | 21413330 |
| medical microbiology | all bacteria, both pathogenic and saprophytic, are unicellular organisms that reproduce by binary fission. most bacteria are capable of independent metabolic existence and growth, but species of chlamydia and rickettsia are obligately intracellular organisms. bacterial cells are extremely small and are most conveniently measured in microns (10-6 m). they range in size from large cells such as ... | 1996 | 21413343 |
| genetic detoxification of bacterial toxins. | several pathogens, such as corynebacterium diphtheriae, clostridium tetani, bordetella pertussis, vibrio cholerae, enterotoxigenic escherichia co1i (1), and even some emerging pathogens, such as helicobacter pylori (2), produce potent toxins that are responsible for the pathology caused by the bacterium. in most cases the disease, and often even the infection, can be prevented by a vaccine that induces immunity against the toxin. in order to be used in vaccines, the dangerous toxins need to be d ... | 1996 | 21359697 |
| accumulation of some secretory enzymes in venom glands of vipera palaestinae. | secretion of venom in the venom glands of vipera palaestinae was studied by measuring the protein content and enzymatic activities of l-amino acid oxidase (lao), phosphodiesterase (pde), and benzoylarginine ethyl esterase (baee). these were tested in the accumulating venom and gland homogenates at 0, 2, 3, 4,6, and 15 days after an intitial emptying of the venom glands by milking. changes in the total activities of the enzymes and in the protein concentration were found in the venom samples, but ... | 1996 | 174447 |
| ovarian tumours in childhood: a 27-year review. | twenty-five children treated for ovarian neoplasms at the royal hospital for sick children, yorkhill,glasgow, between 1947 and 1974 are reported. eight of the patients had malignant tumours and 17 benign. the patients with benign tumours were treated by either salpingo-oophorectomy or oophorectomy. the malignant cases were treated with either surgery alone or in combination with deep x-ray therapy and/or chemotherapy. two patients died of malignant teratoma, but the remaining 6 with malignant te ... | 1996 | 178398 |
| receptor-mediated control of cholesterol metabolism. | 1996 | 174194 | |
| the subcellular distribution and regulation of platelet phosphoprotein phosphatase [proceedings]. | 1996 | 229016 | |
| [biopsy and morphology]. | 1996 | 174513 | |
| [psychosomatics in the nursing field]. | 1996 | 229313 | |
| the nasal fundus ectasia. | the object of this work has been to describe an eye anomaly consisting of a series of individual characteristics. as a name for the disorder "the nasal fundus ectasia" has been selected. special attention has been paid to the bitemporal visual field defects which can easily lead to a suspicion of tumour in the region of the optic chiasm. | 1996 | 174383 |
| [electron microscopy of malignant synovialoma]. | 1996 | 174333 | |
| resistance to receptor-mediated degradation of a murine epidermal growth factor analogue (egf-val-47) potentiates its mitogenic activity. | in most cell types two classes of epidermal growth factor (egf) receptors can be found: a major class that binds egf with relatively low affinity and a minor class that binds with very high affinity. structure-function studies have shown that mutations at amino acid 47 in the egf molecule severely reduce its affinity for the egf receptor but do not cause preferential binding to one or the other subclass of receptors. using three egf derivatives with a mutation at amino acid 47 (ser-47, leu-37-ty ... | 1996 | 2271672 |
| [urogenital tuberculosis in antituberculosis dispensary practice]. | the investigations carried out within the district depending upon the dispensary showed that urogenital tuberculosis incidence is in the second place, after respiratory tuberculosis; in 47% of the cases it was the only manifestation of the disease, in the other cases it was secondary to or accompanied other tuberculous localizations. | 1996 | 174176 |
| alterations in creatinine clearance during respiratory distress syndrome. | some renal parameters have been studied in newborns with respiratory distress syndrome (rds). during the first 24 h, the serum creatinine level of the severe cases of rds are significantly increased, decreasing gradually with improvement of the rds and reaching normal values within 10 days. blood urea nitrogen remained unchanged. in the milder cases of rds, the serum creatinine also showed a slighter increase, which became normal within 4 days after birth. during the acute phase of rds, there is ... | 1996 | 38862 |
| critical review of tomography in radiology and nuclear medicine. | this review covers conventional radiographic tomography, radioisotopic tomography, and a review of computerized transaxial tomography. simple, reproducible radiographic tomographic methods are increasing in use, and despite their complexity, the diagnostic results are superior to conventional radiographs. there are many different motions of the x-ray tube and the film which can be employed to create a tomogram. the perfect tomographic motion is partly determined by the geometric shape of the obj ... | 1996 | 168033 |
| ultrasonic foetal head circumference as a means of assessing gestational age in nigerians. | in a cross-sectional study, we have established the foetal head circumference in pregnant nigerian women between the 16th to the 40th weeks of gestation. the relationship between the head circumference and gestational age is linear although there is some slight slowing of the growth rate after 32 weeks. a linear mathematical model was shown to describe the relationship between gestational age and head circumference and with the knowledge of one the other could be determined. the values obtained ... | 1996 | 2271418 |
| subcellular distribution of protein kinase activities in mammalian islets of langerhans [proceedings]. | 1996 | 229041 | |
| lead, mercury, and cadmium pollution. | 1996 | 174063 | |
| [obstetric complications in the ehlers-danlos syndrome]. | after discussing the so-called ehlers-danlos syndrome in connection with pregnancy and birth, we report on a complication, turning out deadly for mother and child. futheron, we discuss the appropriate control of pregnancy and birth. | 1996 | 1217253 |
| location of cr(iii) in the cr(iii)--cytochrome c complex as observed by nmr spectroscopy. | 1996 | 228980 | |
| the management of craniofacial pain in a pain relief unit. | craniofacial pain can be one of the more intractable problems that presents to the gdp. one management route can be referral to a specialist pain relief unit, where the facilities and expertise will be available for diagnosis, counselling, drug management and invasive therapy. in this article the authors describe the results achieved at one such unit, over a one-year period, for a group of 34 craniofacial pain sufferers. at the end of the year 30 patients reported total or partial relief from th ... | 1996 | 2639826 |
| cyclic nucleotides and cyclic nucleotide phosphodiesterase during development of polysphondylium violaceum. | 1996 | 228950 | |
| 20 years experience in chemotherapy of choriocarcinoma and malignant mole. | 1996 | 228913 | |
| [small cell anaplastic lung cancer--clinical features and therapy]. | 1996 | 173943 | |
| psychophysiological reactions as a function of life stress and behavioral rigidity. | studies have shown that vulnerability to disease is a function of psychosocial factors. this study hypothesized that disease onset is antedated by life changes and is influenced by the individual's "behavioral rigidity." the subjects were 160 female college students from three undergraduate general elective courses. an index of life change (lci) was administered to each subject, presenting descriptions of important pressures and conflicts, with instruction that she indicate which ones she had pe ... | 1996 | 177772 |
| barriers to research in psychiatric-mental health nursing: implications for preparing the nurse researcher. | 1996 | 177768 | |
| an improved method for the extraction of endogenous platelet serotonin. | investigations of blood platelet serotonin content, turnover, metabolism, and secretion require sensitive methods for accurate measurement of the amine. the present study has sought a simple, efficient procedure for extracting total platelet serotonin which could also be used for analysis of other platelet chemical constituents. znso4 precipitation, the standard technique for extracting 5-ht, failed to recover total platelet serotonin despite physical and chemical manipulations to improve its av ... | 1996 | 173765 |
| effects of temperature and salinity on survival of toxigenicvibrio cholerae o1 in seawater. | in 1991 and 1992, the latin american epidemic strain of vibrio cholerae o1 was isolated from ballast water, bilge water, and sewage taken from cargo ships docked in mobile bay, alabama. the findings raised questions regarding the organism's ability to survive long-term aboard ships and to withstand the exchange of ballast at sea. the effects of temperature (6, 18, and 30°c) and salinity (8, 16, and 32 ppt) on survival of v. cholerae o1 strains c6706 and c6707 and a ballast water isolate in steri ... | 1996 | 24185740 |
| viable but non-culturable vibrio cholerae o1 revert to a cultivable state in the human intestine. | vibrio cholerae o1 can enter a state in which they remain viable but are non-culturable. presumably, such bacteria can be pathogenic if they retain the capacity to proliferate in the human intestine following ingestion. two groups of volunteeers were given inocula containing viable but non-culturable v. cholerae o1 of the attenuated vaccine strain cvd 101 (viable cvd 101 organisms readily colonize the human intestine). volunteers in one of the two groups excreted viable cvd 101, demonstrating th ... | 1996 | 24415083 |
| immunochemistry and comparative insect (neuro) endocrinology. | 1995 | 2200010 | |
| size and distribution of sv 40 dna sequences covalently linked with the dna of permissive mammalian cells. | cv-1 cells productively infected with sv 40 contain viral dna which is covalently linked with the host cell dna. these linear duplex viral-host dna molecules are replicated during the infectious cycle. they can be selectively isolated and purified by two successive cycles of dna-dna hybridization and elution steps using first cv-1 cell and then sv 40-dna immobilized on filters. in an attempt to clarify the nature of the host dna sequences neighbouring the viral dna it was found that reiterated h ... | 1995 | 167530 |
| cofactor recycling in liquid membrane-enzyme systems. | 1995 | 177010 | |
| a chemical approach for the localization of membrane sites involved in lymphocyte activation. | 1995 | 177007 | |
| pain suppression after pulpectomy with preoperative flurbiprofen. | 1995 | 3327906 | |
| on the usefulness of pas reaction for the prognosis of acute lymphoblastic leukemia. | 1995 | 173631 | |
| cell surface sialylation plays a role in modulating sensitivity towards apo-1-mediated apoptotic cell death. | apo-1/fas(cd95), a member of the tumour necrosis factor (tnf)/nerve growth factor (ngf) receptor superfamily transduces apoptotic signals into apoptosis sensitive cells. in metabolic labelling experiments using the highly apo-1 positive cell lines hut78 (adultt cell leukemia) and skw6.4 (blymphoblastoid cell line) apo-1 was characterised as a long living protein with a complex glycosylation pattern involving terminal sialic acid groups which account for 8-kda of its apparent molecular weight on ... | 1995 | 17180039 |
| high cell density fermentation of recombinant vibrio cholerae for the production of b subunit of escherichia coli enterotoxin. | high cell density fermentation studies were performed to produce the b subunit of escherichia coli heat-labile enterotoxin (ltb) from a vibrio cholerae culture that carries a recombinant plasmid with an ampicillin resistance gene, tac promoter, and the gene encoding ltb. upon induction with isopropyl-beta-d-thiogalactopyranoside (iptg) the culture secreted the protein into the extracellular milieu. fed-batch fermentation with stepwise addition of a total of 5 mm of iptg during the active growth ... | 1995 | 18623144 |
| viability of vibrio vulnificus in association with hemocytes of the american oyster (crassostrea virginica). | certain indigenous estuarine bacteria, such as vibrio vulnificus, may cause opportunistic human infections after consumption of raw oysters or exposure of tissues to seawater. v. vulnificus is known to be closely associated with oyster (crassostrea virginica) tissues and is not removed by controlled purification methods, such as uv light-assisted depuration. in fact, when live shellfish are subjected to controlled purification, the number of v. vulnificus cells can markedly increase. a review of ... | 1995 | 16534922 |
| cholera in the mediterranean: outbreak in albania. | in the 1990s, an epidemic of cholera caused by vibrio cholerae 01 el tor has spread from northern pakistan to the mediterranean. in 1993 sporadic cases of cholera, and epidemic foci, were recorded in many countries of the southern part of eastern europe. | 1995 | 12631749 |
| genomic and biochemical relatedness between vibrio cholerae serovar o139 and serovar o1 eltor strains. | vibrio cholerae o139 (bengal) the new pandemic cholera strain emerging on the indian subcontinent has revealed considerable homology to vibrio cholerae o1 el tor (strain of the seventh pandemic cholera) in terms of genetic and biochemical properties. apart from capsule and o139 lps formation, all strains of v. cholerae o139 were found to be identical to v. cholerae o1 el tor strains with respect to genomic restriction fragment length polymorphism, genomic distribution of the pathogenic island, p ... | 1995 | 9810642 |
| nontoxigenic vibrio cholerae o1 intestinal pathology in adult mice. | the intestinal pathology caused by nontoxigenic vibrio cholerae o1 was examined in the sealed adult mouse (sam) model. histologic examination demonstrated that a nontoxigenic v. cholerae o1 strain that elicited maximum fluid accumulation (fa) in the small intestine of adult mice caused damages to the villi and necrosis of lymphoid elements within solitary submucosal lymphoid nodules in the peyer's patches. challenge of mice with a strain that did not elicit intestinal fa produced none of the abo ... | 1995 | 9810644 |
| molecular epidemiological studies of vibrio cholerae in taiwan: genotyping by polymerase chain reaction and dna sequencing. | to type the vibrio cholerae strains isolated from sporadic and epidemic cases in taiwan, 28 toxigenic isolates were studied by sequencing polymerase chain reaction-amplified cholera toxin gene (ctx) fragments. based on specific base substitutions on positions 115 and 203 of ctxb and comparison with previously published typing system from centers for disease control and prevention (olsvik theta et al., j clin microbiol 1993; 31:22-5, ref.1), two genotypes were identified. cholera strains from imp ... | 1995 | 9775006 |
| a review of the current status of enteric vaccines. | much progress has been made in developing vaccines against the most important enteric infections. two new vaccines against typhoid fever (oral ty21a and parenteral vi polysaccharide) have been licensed in many countries. newer, more sophisticated typhoid vaccines undergoing clinical testing include recombinant attenuated salmonella typhi strains and vi polysaccharide-carrier-protein conjugate vaccines. two inactivated oral cholera vaccines, consisting of inactivated vibrio cholerae o1 bacteria a ... | 1995 | 9522876 |
| neonatal diarrhea caused by vibrio cholerae 0139 bengal. | cholera rarely occurs in children under 2 years of age. we describe diarrhea due to vibrio cholerae 0139 bengal, the newly described etiologic agent of cholera in a 4-day-old breast-fed baby. however, the diarrhea was mild and was successfully treated with rehydration therapy and erythromycin. | 1995 | 9407221 |
| intestinal coinfection with numerous giardia trophozoites and vibrio cholerae in hospitalized children with watery diarrhea. | during the recent cholera epidemic which affected peru and other latin american countries, fresh stool samples of 100 hospitalized children were assessed february through april 1991. the children had been admitted because of profuse watery diarrhea. the microbiologic study of wet mount preparations showed curved bacteria suspicious for cholera agent and confirmed afterward to be vibrio cholerae 01 scrotype inaba. in 30% of such cases, besides the curved bacteria, a strikingly large number of tro ... | 1995 | 11995904 |
| an imported cholera case infected with both o139 synonym bengal and o1 vibrio cholerae in japan. | 1995 | 8861860 | |
| prescribing pattern by doctors for acute diarrhoea in children in delhi, india. | parents (mostly mothers) of 264 children aged less than 5 years with acute watery diarrhoea were interviewed about their treatment profile before hospitalization in delhi, india in 1993. only 22% of the cases were given prescriptions for oral rehydration solutions (ors), whereas a very high proportion (64%) of them were given drugs, including antibiotics and antidiarrhoeals and 40% were given intravenous fluids. the differences among the treatment groups were highly significant. the government a ... | 1995 | 8838825 |
| a natural vaccine candidate strain against cholera. | e1 tor vibrio cholerae (evc) strains may be classified into two kinds-epidemigenic (eevc) strains and non-epidemigenic (neevc) strains-based on a phage-biotyping system. a large number of eevc strains have been screened for toxigenic and putative colonization attributes. one such naturally occurring strains (designated iem101) has been found which is devoid of genes encoding cholera toxin (ct), accessory cholera enterotoxin (ace), zonula occludens toxin (zot), but possesses rs1 sequences and tox ... | 1995 | 8719177 |
| [antibiotic sensitivity to epidemic strains of vibrio cholerae and shigella dysenteriae 1 isolated in rwandan refugee camps in zaire]. | multiresistance or epidemic enteric bacteria to antibiotics greatly complicates treatment, and in some cases prophylaxis, of severe invasive gastroenteritis. during the summer of 1994, two epidemics of diarrhea, one due to vibrio cholerae and the other to shigella dysenteriae 1 isolated from the goma and bukavu camps was determined by measurement of the agar minimal inhibitory concentration. multiresistance to tetracyclins, aminopenicillins, trimethoprimsulfamethoxazole, and nifuroxazide was obs ... | 1995 | 8830219 |
| use of recombinase gene fusions to identify vibrio cholerae genes induced during infection. | a complete understanding of host-parasite interactions must necessarily include the identification and characterization of gene products expressed by both parties during the infectious process. we have developed a new screen to identify bacterial genes that are transcriptionally induced during infection of a host animal. the method is based on pre-selection of strains carrying tnpr operon fusions (encoding resolvase, a site-specific dna recombinase) which are not expressed in vitro, followed by ... | 1995 | 8817490 |
| characterization of vibrio cholerae bacteriophage k139 and use of a novel mini-transposon to identify a phage-encoded virulence factor. | temperate bacteriophage k139 was isolated from a vibrio cholerae o139 isolate and characterized in this study. the phage genome consists of a 35 kbp, double-stranded, linear dna molecule that circularizes and integrates into the chromosome in a site-specific manner. dna sequences that cross-hybridize with k139 phage dna are present in all strains of v. cholerae serogroup o1 of the classical biotype examined and in some strains of the el tor biotype. phage k139 produces plaques on el tor o1 strai ... | 1995 | 8817491 |
| atypical infection due to vibrio cholerae in patients infected with human immunodeficiency virus. | 1995 | 8749654 | |
| prevalence of vibrio cholerae and salmonella in a major shrimp production area in thailand. | in 1992 and 1993, a 7 months study carried out in a major shrimp-producing area in southern thailand to study the prevalence of vibrio cholerae and salmonella. a total of 158 samples were examined including water, sediment, shrimp, pelleted feed, shrimp gut, and chicken manure. salmonella was not recovered from any sample type studied. v. cholerae o1 was isolated from 2 (2%) and v. cholerae non-o1 was isolated from 35 (33%) of 107 samples examined. the occurrence of v. cholerae was not significa ... | 1995 | 8751094 |
| vibrio cholerae 0139 diarrhea and acute renal failure in a three-day-old infant. | 1995 | 8786900 | |
| clinical features of infantile diarrhea associated with single or multiple enteric pathogens. | clinical features of infantile diarrhea were studied among 603 infants from birth to 12 months of age to determine the predominant clinical feature(s) seen in infantile diarrhea associated with a specific enteric pathogen. among the major clinical features, fever was most often seen in diarrhea due to yersinia spp. (61.5%) followed by that in rotavirus (26.1%). vomiting was mostly associated with vibrio cholerae infection (90.9%) and shigellosis (64.6%). dehydration was predominant in vibrio cho ... | 1995 | 8714622 |
| restriction fragment length polymorphisms (rflp) of cholera toxin genes in vibrio cholerae o139 recovered from patients in thailand, india and bangladesh. | since its first appearance in 1992, vibrio cholerae o139 has caused large epidemics of a cholera-like disease in india and bangladesh and has subsequently spread to several neighboring countries. we characterized and compared 56 v. cholerae o139 isolates recovered from patients in thailand, india and bangladesh by analyzing restriction fragment length polymorphisms (rflp) of their ctx genes. the strains comprised 9 different bgli cleavage patterns of ctx genes (ct genotypes) and contained 1-4 ge ... | 1995 | 8685638 |
| [vibrios pathogenic to humans and laboratory diagnosis of diseases caused by them]. | present-day taxonomic status of vibrios pathogenic for humans is discussed, as are the phenotypical characteristics of some species, factors of virulence, and clinical manifestations of diseases caused by these agents. a scheme of isolation and identification of vibrios pathogenic for humans is offered. | 1995 | 8689064 |
| [bacteriological aspects of cholera in chad (1991 and 1994 epidemics)]. | 1995 | 8640086 | |
| [acute renal failure as a complication of cholera]. | we present a 72-year-old man who had episodes of severe, acute renal failure during severe attacks of diarrhea caused by vibrio cholerae. patterns of acute tubular necrosis and tubulointerstitial nephritis developed following hypotension and decrease in renal blood flow, causing secondary renal ischemia. there was severe dehydration with profound hypovolemia and infection. the clinical picture included fever, weakness, arthralgia, pedal edema, mild bilateral pleural effusions, anemia, leukocytos ... | 1995 | 8682355 |
| major enteropathogenic bacteria isolated from diarrheal patients in bolivia: a hospital-based study. | a total of 1,234 fecal samples from diarrhea cases were examined for etiological bacterial agents at medical facilities in la paz and sucre, bolivia. eighty strains of shigella spp., 39 strains of salmonella spp., 29 strains of vibrio cholerae, and 222 strains of enteropathogenic escherichia coli (139 epec, 55 etec, 29 eiec, and 1 ehec) were isolated. with regard to the serovars of shigella, s. flexneri 2a, 3a, and 1b were predominant. in the case of salmonella, s. enteritidis was the most commo ... | 1995 | 8657011 |