Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| glycoproteins from insect cells: sialylated or not? | our growing comprehension of the biological roles of glycan moieties has created a clear need for expression systems that can produce mammalian-type glycoproteins. in turn, this has intensified interest in understanding the protein glycosylation pathways of the heterologous hosts that are commonly used for recombinant glycoprotein expression. among these, insect cells are the most widely used and, particularly in their role as hosts for baculovirus expression vectors, provide a powerful tool for ... | 0 | 11308014 |
| epidemiological characteristics and risk factors of dengue infection in korean travelers. | dengue viral infection has rapidly spread around the world in recent decades. in korea, autochthonous cases of dengue fever have not been confirmed yet. however, imported dengue cases have been increased since 2001. the risk of developing severe dengue in korean has been increased by the accumulation of past-infected persons with residual antibodies to dengue virus and the remarkable growth of traveling to endemic countries in southeast asia. notably, most of imported dengue cases were identifie ... | 0 | 27822922 |
| mosquito-specific microrna-1890 targets the juvenile hormone-regulated serine protease jha15 in the female mosquito gut. | females of the hematophagous mosquito species require a vertebrate blood meal to supply amino acids and other nutrients necessary for egg development, serving as the driving force for the spread of many vector-borne diseases in humans. blood digestion utilizes both early and late phase serine proteases (sps) that are differentially regulated at the transcriptional and post-transcriptional level. to uncover the regulatory complexity of sps in the female mosquito midgut, we investigated involvemen ... | 0 | 26488481 |
| accelerated evolution of constraint elements for hematophagic adaptation in mosquitoes. | comparative genomics is a powerful approach that comprehensively interprets the genome. herein, we performed whole genome comparative analysis of 16 diptera genomes, including four mosquitoes and 12 drosophilae. we found more than 540 000 constraint elements (ces) in the diptera genome, with the majority found in the intergenic, coding and intronic regions. accelerated elements (aes) identified in mosquitoes were mostly in the protein-coding regions (>93%), which differs from vertebrates in geno ... | 0 | 26646568 |
| development of johnston's organ in drosophila. | hearing is a specialized mechanosensory modality that is refined during evolution to meet the particular requirements of different organisms. in the fruitfly, drosophila, hearing is mediated by johnston's organ, a large chordotonal organ in the antenna that is exquisitely sensitive to the near-field acoustic signal of courtship songs generated by male wing vibration. we summarize recent progress in understanding the molecular genetic determinants of johnston's organ development and discuss surpr ... | 0 | 17891726 |
| dna transposons and the evolution of eukaryotic genomes. | transposable elements are mobile genetic units that exhibit broad diversity in their structure and transposition mechanisms. transposable elements occupy a large fraction of many eukaryotic genomes and their movement and accumulation represent a major force shaping the genes and genomes of almost all organisms. this review focuses on dna-mediated or class 2 transposons and emphasizes how this class of elements is distinguished from other types of mobile elements in terms of their structure, ampl ... | 0 | 18076328 |
| revolutionary fever: disease and war in the lower south, 1776-1783. | 0 | 18528506 | |
| photoirradiation of polycyclic aromatic hydrocarbons with uva light - a pathway leading to the generation of reactive oxygen species, lipid peroxidation, and dna damage. | polycyclic aromatic hydrocarbons (pahs) are a class of genotoxic environmental contaminants. we have long been interested in determining the mechanisms by which pahs induce genotoxicity. although the metabolic activation of pahs leading to biological activities has been well studied, the photo-induced activation pathway has seldom reported. in this paper, we review the study of photoirradiation of pahs with uva irradiation results in (i) cytotoxicity and dna damage (ii) dna single strand cleavag ... | 0 | 17159277 |
| community based vector control in malindi, kenya. | community involvement has become an important component of the national malaria control strategy in kenya, resulting in the organization of groups charged with addressing mosquito and malaria-related concerns within the community. | 0 | 17604514 |
| rnai knockdown of a salivary transcript leading to lethality in the pea aphid, acyrthosiphon pisum. | abstract injection of sirna (small interfering rna) into parthenogenetic adult pea aphids (acyrthosiphon pisum) is shown here to lead to depletion of a target salivary gland transcript. the sirna was generated from double stranded rna that covered most of the open reading frame of the transcript, which we have called coo2. the coo2 transcript level decreases dramatically over a 3-day period after injection of sirna. with a lag of 1 to 2 days, the sicoo2-rna injected insects died, on average 8 da ... | 0 | 20233093 |
| genomic organization and developmental expression of glutathione s-transferase genes of the diamondback moth, plutella xylostella. | in the present study, we cloned and sequenced the entire coding regions of two glutathione s-transferase (gst) genes encoding pxgsts and pxgste from the diamondback moth, plutella xylostella l. (lepidoptera: yponomeutidae), along with their respective 5' and 3' flanking regions. the pxgsts gene was composed of four exons and three introns. based on amino acid identity with gst genes from other insects, this gene was classified as a member of the sigma class. the gene encoding pxgste had an intro ... | 0 | 20233073 |
| response of culex quinquefasciatus larvae to three insecticides. | 0 | 27049713 | |
| predicting wolbachia potential to knock down dengue virus transmission. | releasing mosquitoes infected with the intracellular bacteria wolbachia is a candidate strategy for dengue control that has recently advanced to field-testing. a critical next step is to evaluate the impact of this strategy on dengue epidemiology. a recent study by ferguson and colleagues presents a mathematical framework to predict the likely effect of mosquitoes carrying wolbachia on dengue virus transmission. fitting the mathematical model to empirical data obtained with wolbachia-infected mo ... | 0 | 26697448 |
| essential oil composition, antimicrobial and pharmacological activities of lippia sidoides cham. (verbenaceae) from são gonçalo do abaeté, minas gerais, brazil. | lippia sidoides (verbenaceae) is used in brazilian folk medicine as an antiseptic, and it is usually applied topically on skin, mucous membranes, mouth, and throat, or used for vaginal washings. | 0 | 27867267 |
| trends in clinical trials of dengue vaccine. | dengue is one of the most important vector-borne disease and an increasing problem worldwide because of current globalization trends. roughly, half the world's population lives in dengue endemic countries, and nearly 100 million people are infected annually with dengue. india has the highest burden of the disease with 34% of the global cases. in the context of an expanding and potentially fatal infectious disease without effective prevention or specific treatment, the public health value of a pr ... | 0 | 27843790 |
| 0 | 27737980 | ||
| reining in angola's yellow fever outbreak. | rosa moreira tells andréia azevedo soares how angola reined in its worst yellow fever epidemic in 30 years. | 0 | 27843160 |
| functional expression and characterization of aedes aegypti dopachrome conversion enzyme. | a full-length mosquito dopachrome conversion enzyme (dce) and its truncated form lacking the last 54 carboxyl-terminal amino acid residues are expressed using a baculovirus/insect cell expression system. the full-length recombinant dce displayed multiple bands during native page with substrate staining, but only one active band was detected when the truncated recombinant dce was analyzed under identical analysis conditions. our data suggest that the last 50 some carboxyl-terminal residues are in ... | 0 | 11779167 |
| testing for context-dependence in a processing chain interaction among detritus-feeding aquatic insects. | scirtid beetles may benefit mosquitoes ochlerotatus triseriatus (say) by consuming whole leaves and leaving behind fine particles required by mosquito larvae. such interactions based on the sequential use of a resource that occurs in multiple forms are known as processing chains.models of processing chains predict that interactions can vary from commensal (0, +) to amensal (0, -), depending on how quickly resource is processed in the absence of consumers.the scirtid-o. triseriatus system was use ... | 0 | 19060960 |
| comparative characterization of aedes 3-hydroxykynurenine transaminase/alanine glyoxylate transaminase and drosophila serine pyruvate aminotransferase. | this study describes the comparative analysis of two insect recombinant aminotransferases, aedes aegypti 3-hydroxykynurenine (3-hk) transaminase/alanine glyoxylate aminotransferase (ae-hkt/agt) and drosophila melanogaster serine pyruvate aminotransferase (dm-spat), which share 52% identity in their amino acid sequences. both enzymes showed agt activity. in addition, ae-hkt/agt is also able to catalyze the transamination of 3-hk or kynurenine with glyoxylate, pyruvate or oxaloacetate as the amino ... | 0 | 12220660 |
| venezuela launches new campaign against dengue fever. | 0 | 12219753 | |
| the impact of genomics on global health. | ensuring that advances in genomics are applied to the health improvement of people living in developing countries is an important contemporary challenge. in the near term, such advances are likely to alleviate infectious diseases, with longer-term benefits envisaged for chronic disorders. to ensure that benefits are shared by developing countries, attention must be paid to complex ethical, legal, social, and economic issues, as well as to public education and engagement. creative and equitable i ... | 0 | 12084683 |
| the history of dengue outbreaks in the americas. | dengue is a viral disease usually transmitted by aedes aegypti mosquitoes. dengue outbreaks in the americas reported in medical literature and to the pan american health organization are described. the outbreak history from 1600 to 2010 was categorized into four phases: introduction of dengue in the americas (1600-1946); continental plan for the eradication of the ae. aegypti (1947-1970) marked by a successful eradication of the mosquito in 18 continental countries by 1962; ae. aegypti reinfesta ... | 0 | 23042846 |
| acute and subacute oral toxicity of litsea elliptica blume essential oil in rats. | litsea elliptica blume has been traditionally used to treat headache, fever, and stomach ulcer, and has also been used as an insect repellent. the acute and subacute toxicities of l. elliptica essential oil were evaluated orally by gavage in female sprague-dawley rats. for the acute toxicity study, l. elliptica essential oil was administered in doses from 500 to 4000 mg/kg (single dose), and in the subacute toxicity test, the following doses were used: 125, 250, and 500 mg/kg, for 28 consecutive ... | 0 | 23024045 |
| study of the fragmentation of arginine isobutyl ester applied to arginine quantification in aedes aegypti mosquito excreta. | it has been demonstrated that argininolysis and uricolysis are involved in the synthesis and excretion of urea in aedes aegypti female mosquitoes. to further investigate the metabolic regulation of urea in female mosquitoes, it is desirable to have a rapid and efficient method to monitor arginine (arg) concentration in mosquito excreta. thus, a procedure currently used for the identification of arg in urea cycle disorders in newborn babies was adapted to analyze arg in a. aegypti excreta. the fr ... | 0 | 23019169 |
| light-mediated control of rhodopsin movement in mosquito photoreceptors. | multiple mechanisms contribute to a photoreceptor's ability to adapt to ambient light conditions. the mosquito aedes aegypti expresses the long-wavelength rhodopsin aaop1 in all r1-r6 photoreceptors and most r8 photoreceptors. these photoreceptors alter the cellular location of aaop1 and reorganize their photosensitive rhabdomeric membranes on a daily basis. during daylight periods, aaop1 is excluded from the light-sensitive rhabdomeres and localized to multivesicular bodies (mvbs) within the ph ... | 0 | 23035078 |
| 100 years of the pan american health organization. | 0 | 12453801 | |
| a 3-synapse positive feedback loop regulates the excitability of an interneuron critical for sensitization in the leech. | sensitization of reflexive shortening in the leech has been linked to serotonin (5-ht)-induced changes in the excitability of a single interneuron, the s cell. this neuron is necessary for sensitization and complete dishabituation of reflexive shortening, during which it contributes to the sensory-motor reflex. the s cell does not contain 5-ht, which is released primarily from the retzius (r) cells, whose firing enhances s-cell excitability. here, we show that the s cell excites the r cells, mai ... | 0 | 16571760 |
| acetylcholinesterase genes within the diptera: takeover and loss in true flies. | it has recently been reported that the synaptic acetylcholinesterase (ache) in mosquitoes is encoded by the ace-1 gene, distinct and divergent from the ace-2 gene, which performs this function in drosophila. this is an unprecedented situation within the diptera order because both ace genes derive from an old duplication and are present in most insects and arthropods. nevertheless, drosophila possesses only the ace-2 gene. thus, a secondary loss occurred during the evolution of diptera, implying ... | 0 | 17002944 |
| infectious disease management: lessons from cuba. | 0 | 18382630 | |
| a transcriptome analysis of the aedes aegypti vitellogenic fat body. | aedes (stegomyia) aegypti is an important dengue vector in tropical and subtropical zones throughout the world. a transcriptome of ae. aegypti vitellogenic fat bodies is described here. the fat body is a dynamic tissue that participates in multiple biochemical functions of intermediate metabolism. a total of 589 randomly selected cdnas were assembled into 262 clusters based on their primary sequence similarities. the putative translated proteins were classified into categories based on their fun ... | 0 | 19537968 |
| immune pathways and defence mechanisms in honey bees apis mellifera. | social insects are able to mount both group-level and individual defences against pathogens. here we focus on individual defences, by presenting a genome-wide analysis of immunity in a social insect, the honey bee apis mellifera. we present honey bee models for each of four signalling pathways associated with immunity, identifying plausible orthologues for nearly all predicted pathway members. when compared to the sequenced drosophila and anopheles genomes, honey bees possess roughly one-third a ... | 0 | 17069638 |
| expression of insulin pathway genes during the period of caste determination in the honey bee, apis mellifera. | female honeybees have two castes, queens and workers. developmental fate is determined by larval diet. coding sequences made available through the honey bee genome sequencing consortium allow for a pathway-based approach to understanding caste determination. we examined the expression of several genes of the insulin signalling pathway, which is central to regulation of growth based on nutrition. we found one insulin-like peptide expressed at very high levels in queen but not worker larvae. also, ... | 0 | 17069635 |
| silencing of molt-regulating transcription factor gene, cihr3, affects growth and development of sugarcane stem borer, chilo infuscatellus. | rna interference (rnai) is a technology for conducting functional genomic studies and a potential tool for crop protection against insect pests. development of reliable methods for production and delivery of double-stranded rna (dsrna) is the major challenge for efficient pest control. in this study, chilo infuscatellus snellen (crambidae: lepidoptera) was fed with cihr3 dsrna expressed in bacteria or synthesized in vitro. the dsrna ingested by c. infuscatellus successfully triggered silencing o ... | 0 | 23427912 |
| two drosophila deg/enac channel subunits have distinct functions in gustatory neurons that activate male courtship. | trimeric sodium channels of the deg/enac family have important roles in neurons, but the specific functions of different subunits present in heteromeric channels are poorly understood. we previously reported that the drosophila deg/enac subunit ppk25 is essential in a small subset of gustatory neurons for activation of male courtship behavior, likely through detection of female pheromones. here we show that, like mutations in ppk25, mutations in another drosophila deg/enac subunit gene, nope, sp ... | 0 | 22915128 |
| biochemical identification and crystal structure of kynurenine formamidase from drosophila melanogaster. | kfase (kynurenine formamidase), also known as arylformamidase and formylkynurenine formamidase, efficiently catalyses the hydrolysis of nfk (n-formyl-l-kynurenine) to kynurenine. kfase is the second enzyme in the kynurenine pathway of tryptophan metabolism. a number of intermediates formed in the kynurenine pathway are biologically active and implicated in an assortment of medical conditions, including cancer, schizophrenia and neurodegenerative diseases. consequently, enzymes involved in the ky ... | 0 | 22690733 |
| dengue fever in south korea, 2006-2010. | 0 | 22931747 | |
| resistance of aedes aegypti to temephos and adaptive disadvantages. | to evaluate the resistance of aedes aegypti to temephos fersol 1g (temephos 1% w/w) associated with the adaptive disadvantage of insect populations in the absence of selection pressure. | 0 | 25372168 |
| o'nyong-nyong virus infection imported to europe from kenya by a traveler. | 0 | 25271361 | |
| dengue expansion in africa-not recognized or not happening? | an expert conference on dengue in africa was held in accra, ghana, in february 2013 to consider key questions regarding the possible expansion of dengue in africa. four key action points were highlighted to advance our understanding of the epidemiology of dengue in africa. first, dengue diagnostic tools must be made more widely available in the healthcare setting in africa. second, representative data need to be collected across africa to uncover the true burden of dengue. third, established net ... | 0 | 25271370 |
| concurrent malaria and dengue fever: a need for rapid diagnostic methods. | malaria and dengue fever are endemic in the south-east asian region including india. both the illnesses share similar symptomatology, but differ in certain respects such as different- causative organisms and mosquito vector with diverse habitat. hence, concurrent malaria and dengue fever in the same patient is said to be unusual. there have been cases of concurrent malaria and dengue, but they are scarce from highly endemic region like ours. here, we describe three unusual cases of plasmodium vi ... | 0 | 25657963 |
| functional characterizations of residues arg-158 and tyr-170 of the mosquito-larvicidal bacillus thuringiensis cry4ba. | the insecticidal activity of bacillus thuringiensis (bt) cry toxins involves toxin stabilization, oligomerization, passage across the peritrophic membrane (pm), binding to midgut receptors and pore-formation. the residues arg-158 and tyr-170 have been shown to be crucial for the toxicity of bt cry4ba. we characterized the biological function of these residues. in mosquito larvae, the mutants r158a/e/q (r158) could hardly penetrate the pm due to a significantly reduced ability to alter pm permeab ... | 0 | 24286331 |
| an unusual ocular emergency in severe dengue. | dengue, one of the most common mosquito-borne flavivirus diseases affecting humans, is spread by the aedes aegypti mosquito. most people infected with dengue virus are asymptomatic or only have mild symptoms such as an uncomplicated fever; few have more severe features, while in a small proportion it is life-threatening. severe dengue is defined as that associated with severe bleeding, severe organ dysfunction, or severe plasma leakage. ophthalmic manifestations can involve both the anterior and ... | 0 | 25371643 |
| three-way interactions between mosquito population, viral strain and temperature underlying chikungunya virus transmission potential. | interactions between pathogens and their insect vectors in nature are under the control of both genetic and non-genetic factors, yet most studies on mosquito vector competence for human pathogens are conducted in laboratory systems that do not consider genetic and/or environmental variability. evaluating the risk of emergence of arthropod-borne viruses (arboviruses) of public health importance such as chikungunya virus (chikv) requires a more realistic appraisal of genetic and environmental cont ... | 0 | 25122228 |
| dengue spatial and temporal patterns, french guiana, 2001. | to study a 2001 dengue fever outbreak in iracoubo, french guiana, we recorded the location of all patients' homes and the date when symptoms were first observed. a geographic information system (gis) was used to integrate the patient-related information. the knox test, a classic space-time analysis technique, was used to detect spatiotemporal clustering. analysis of the relative-risk (rr) variations when space and time distances vary, highlighted the maximum space and time extent of a dengue tra ... | 0 | 15200850 |
| dengue fever outbreak in a recreation club, dhaka, bangladesh. | an outbreak of dengue fever occurred among employees of a recreation club in bangladesh. occupational transmission was characterized by a 12% attack rate, no dengue among family contacts, and aedes vectors in club areas. early recognition of the outbreak likely limited its impact. | 0 | 15200876 |
| signature of the oligomeric behaviour of nuclear receptors at the sequence and structural level. | nuclear receptors (nrs) are ligand-dependent transcription factors that control a large number of physiological events through the regulation of gene transcription. nrs function either as homodimers or as heterodimers with retinoid x receptor/ultraspiracle protein (rxr/usp). a structure-based sequence analysis aimed at discovering the molecular mechanism that controls the dimeric association of the ligand-binding domain reveals two sets of differentially conserved residues, which partition the e ... | 0 | 15105832 |
| stereodivergent construction of cyclic ethers by a regioselective and enantiospecific rhodium-catalyzed allylic etherification: total synthesis of gaur acid. | 0 | 15366088 | |
| interspecific differences in feeding behavior and survival under food-limited conditions for larval aedes albopictus and aedes aegypti (diptera: culicidae). | aedes albopictus has replaced aedes aegypti in much of the latter species' historic range within the united states. the leading hypothesis for this displacement is exclusion via resource competition; however, the proximate mechanism producing a competitive advantage for a. albopictus over a. aegypti has not been identified. we performed laboratory experiments to test the hypotheses that these species differ in feeding behavior, and that these differences result in differences in survival when re ... | 0 | 23197877 |
| yellow fever: readily prevented but difficult to treat. | 0 | 15210636 | |
| the changing tails of a novel short interspersed element in aedes aegypti: genomic evidence for slippage retrotransposition and the relationship between 3' tandem repeats and the poly(da) tail. | a novel family of trna-related sines named gecko was discovered in the yellow fever mosquito, aedes aegypti. approximately 7200 copies of gecko were distributed in the a. aegypti genome with a significant bias toward a + t-rich regions. the 3' end of gecko is similar in sequence and identical in secondary structure to the 3' end of mosqui, a non-ltr retrotransposon in a. aegypti. nine conserved substitutions and a deletion separate gecko into two groups. group i includes all gecko that end with ... | 0 | 15611173 |
| bracoviruses contain a large multigene family coding for protein tyrosine phosphatases. | the relationship between parasitic wasps and bracoviruses constitutes one of the few known mutualisms between viruses and eukaryotes. the virions produced in the wasp ovaries are injected into host lepidopteran larvae, where virus genes are expressed, allowing successful development of the parasite by inducing host immune suppression and developmental arrest. bracovirus-bearing wasps have a common phylogenetic origin, and contemporary bracoviruses are hypothesized to have been inherited by chrom ... | 0 | 15542661 |
| cysteine and keto acids modulate mosquito kynurenine aminotransferase catalyzed kynurenic acid production. | kynurenine aminotransferase (kat) catalyzes the formation of kynurenic acid (kyna), the natural antagonist of ionotropic glutamate receptors. this study tests potential substrates and assesses the effects of amino acids and keto acids on the activity of mosquito kat. various keto acids, when simultaneously present in the same reaction mixture, display a combined effect on kat catalyzed kyna production. moreover, methionine and glutamine show inhibitory effects on kat activity, while cysteine fun ... | 0 | 15556614 |
| natively unfolded domains in endocytosis: hooks, lines and linkers. | it is commonly assumed that a protein must adopt a tertiary structure to achieve its active native state and that regions of a protein that are devoid of alpha-helix or beta-sheet structures are functionally inert. although extended proline-rich regions are recognized as presenting binding motifs to, for example, src homology 2 (sh2) and sh3 domains, the idea persists that natively unfolded regions in functional proteins are simply 'spacers' between the folded domains. such a view has been chall ... | 0 | 15520805 |
| reappearance of chikungunya, formerly called dengue, in the americas. | after an absence of ≈200 years, chikungunya returned to the american tropics in 2013. the virus is maintained in a complex african zoonotic cycle but escapes into an urban cycle at 40- to 50-year intervals, causing global pandemics. in 1823, classical chikungunya, a viral exanthem in humans, occurred on zanzibar, and in 1827, it arrived in the caribbean and spread to north and south america. in zanzibar, the disease was known as kidenga pepo, swahili for a sudden cramp-like seizure caused by an ... | 0 | 25816211 |
| from genome to "venome": molecular origin and evolution of the snake venom proteome inferred from phylogenetic analysis of toxin sequences and related body proteins. | this study analyzed the origin and evolution of snake venom proteome by means of phylogenetic analysis of the amino acid sequences of the toxins and related nonvenom proteins. the snake toxins were shown to have arisen from recruitment events of genes from within the following protein families: acetylcholinesterase, adam (disintegrin/metalloproteinase), avit, complement c3, crotasin/beta defensin, cystatin, endothelin, factor v, factor x, kallikrein, kunitz-type proteinase inhibitor, lynx/slur, ... | 0 | 15741511 |
| molecular evolution, structure, and function of peroxidasins. | peroxidasins represent the subfamily 2 of the peroxidase-cyclooxygenase superfamily and are closely related to chordata peroxidases (subfamily 1) and peroxinectins (subfamily 3). they are multidomain proteins containing a heme peroxidase domain with high homology to human lactoperoxidase that mediates one- and two-electron oxidation reactions. additional domains of the secreted and glycosylated metalloproteins are type c-like immunoglobulin domains, typical leucine-rich repeats, as well as a von ... | 0 | 22976969 |
| chikungunya outbreak in bueng kan province, thailand, 2013. | 0 | 25061989 | |
| transfusion support in patients with dengue fever. | dengue fever has emerged as a global public health problem in the recent decades. the clinical spectrum of the disease ranges from dengue fever to dengue hemorrhagic fever and dengue shock syndrome. the disease is characterized by increased capillary permeability, thrombocytopenia and coagulopathy. thrombocytopenia with hemorrhagic manifestations warrants platelet transfusions. there is lack of evidence-based guidelines for transfusion support in patients with dengue fever. this contributes to i ... | 0 | 25298950 |
| laboratory evaluation of the development of aedes aegypti in two seasons: influence of different places and different densities. | aedes aegypti is an important vector in brazil being the main vector of the dengue-fever. this paper employs survival curves to describe the time in days from larvae to adult forms of aedes aegypti raised, individually and collectively, and compares it during winter and spring when positioned inside and outside a laboratory. the study was conducted in são vicente, a coastal city in southeastern brazil. the lowest water temperature in winter and in spring was 20 °c and the highest was 26 °c in sp ... | 0 | 25229215 |
| detection and characterization of wolbachia infection in silkworm. | wolbachia naturally infects a wide variety of arthropods, where it plays important roles in host reproduction. it was previously reported that wolbachia did not infect silkworm. by means of pcr and sequencing we found in this study that wolbachia is indeed present in silkworm. phylogenetic analysis indicates that wolbachia infection in silkworm may have occurred via transfer from parasitic wasps. furthermore, southern blotting results suggest a lateral transfer of the wsp gene into the genomes o ... | 0 | 25249781 |
| double trouble: combined action of meiotic drive and wolbachia feminization in eurema butterflies. | arthropod sex ratios can be manipulated by a diverse range of selfish genetic elements, including maternally inherited wolbachia bacteria. feminization by wolbachia is rare but has been described for eurema mandarina butterflies. in this species, some phenotypic and functional females, thought to be zz genetic males, are infected with a feminizing wolbachia strain, wfem. meanwhile, heterogametic wz females are not infected with wfem. here, we establish a quantitative pcr assay allowing reliable ... | 0 | 25948567 |
| chile confronts its environmental health future after 25 years of accelerated growth. | chile has recently been reclassified by the world bank from an upper-middle-income country to a high-income country. there has been great progress in the last 20 to 30 years in relation to air and water pollution in chile. yet after 25 years of unrestrained growth, there remain clear challenges posed by air and water pollution, as well as climate change. | 0 | 26615070 |
| chikungunya outbreak in garo hills, meghalaya: an epidemiological perspective. | chikungunya (chik) fever is a mosquito-borne disease caused by chikungunya virus (chikv). chikungunya infection was first reported from india in 1963 from kolkata. we report the serological and molecular evidence of an outbreak of chikungunya in northeast india that occurred in tura, a hilly and forested terrain in garo hills district of meghalaya. | 0 | 26139776 |
| climate change influences on global distributions of dengue and chikungunya virus vectors. | numerous recent studies have illuminated global distributions of human cases of dengue and other mosquito-transmitted diseases, yet the potential distributions of key vector species have not been incorporated integrally into those mapping efforts. projections onto future conditions to illuminate potential distributional shifts in coming decades are similarly lacking, at least outside europe. this study examined the global potential distributions of aedes aegypti and aedes albopictus in relation ... | 0 | 25688023 |
| dengue: recent past and future threats. | this article explores four key questions about statistical models developed to describe the recent past and future of vector-borne diseases, with special emphasis on dengue: (1) how many variables should be used to make predictions about the future of vector-borne diseases? (2) is the spatial resolution of a climate dataset an important determinant of model accuracy? (3) does inclusion of the future distributions of vectors affect predictions of the futures of the diseases they transmit? (4) whi ... | 0 | 25688021 |
| present and future projections of habitat suitability of the asian tiger mosquito, a vector of viral pathogens, from global climate simulation. | climate change can influence the transmission of vector-borne diseases (vbds) through altering the habitat suitability of insect vectors. here we present global climate model simulations and evaluate the associated uncertainties in view of the main meteorological factors that may affect the distribution of the asian tiger mosquito (aedes albopictus), which can transmit pathogens that cause chikungunya, dengue fever, yellow fever and various encephalitides. using a general circulation model at 50 ... | 0 | 25688015 |
| how mathematical epidemiology became a field of biology: a commentary on anderson and may (1981) 'the population dynamics of microparasites and their invertebrate hosts'. | we discuss the context, content and importance of the paper 'the population dynamics of microparasites and their invertebrate hosts', by r. m. anderson and r. m. may, published in the philosophical transactions of the royal society as a stand-alone issue in 1981. we do this from the broader perspective of the study of infectious disease dynamics, rather than the specific perspective of the dynamics of insect pathogens. we argue that their 1981 paper fits seamlessly in the systematic study of inf ... | 0 | 25750231 |
| spatiotemporal distribution of dengue vectors & identification of high risk zones in district sonitpur, assam, india. | dengue is an arboviral disease of public health importance in many parts of india and recently many cases have been reported from northeastern india. aedes mosquitoes, which are the vectors of dengue, are widely prevalent in the region. a study was initiated in sonitpur district of assam to understand the spatiotemporal distribution and seasonal prevalence of dengue vectors and to identify the high risk zones. | 0 | 25297362 |
| bioactivity of indigenous medicinal plants against the cotton whitefly, bemisia tabaci. | forty-one methanol extracts of 28 indigenous medicinal plant species were tested for their insecticidal bioactivity against cotton whitefly, bemisia tabaci (gennadius) (hemiptera: aleyrodidae), adults and second nymphal instars under controlled conditions. this study is within a bioprospection context, in the form of utilizing local plant species as an alternative in sustainable agriculture development. eighteen and nine plant extracts caused a significant decrease in number of live adult and ny ... | 0 | 25204756 |
| expression of a toll signaling regulator serpin in a mycoinsecticide for increased virulence. | serpins are ubiquitously distributed serine protease inhibitors that covalently bind to target proteases to exert their activities. serpins regulate a wide range of activities, particularly those in which protease-mediated cascades are active. the drosophila melanogaster serpin spn43ac negatively controls the toll pathway that is activated in response to fungal infection. the entomopathogenic fungus beauveria bassiana offers an environmentally friendly alternative to chemical pesticides for inse ... | 0 | 24837378 |
| a mosquito sperm's journey from male ejaculate to egg: mechanisms, molecules, and methods for exploration. | the fate of mosquito sperm in the female reproductive tract has been addressed sporadically and incompletely, resulting in significant gaps in our understanding of sperm-female interactions that ultimately lead to fertilization. as with other diptera, mosquito sperm have a complex journey to their ultimate destination, the egg. after copulation, sperm spend a short time at the site of insemination where they are hyperactivated and quickly congregate near the entrance of the spermathecal ducts. w ... | 0 | 27147424 |
| inner workings: a big role for a microbial parasite. | 0 | 27095842 | |
| insights into the venom composition of the ectoparasitoid wasp nasonia vitripennis from bioinformatic and proteomic studies. | with the nasonia vitripennis genome sequences available, we attempted to determine the proteins present in venom by two different approaches. first, we searched for the transcripts of venom proteins by a bioinformatic approach using amino acid sequences of known hymenopteran venom proteins. second, we performed proteomic analyses of crude n. vitripennis venom removed from the venom reservoir, implementing both an off-line two-dimensional liquid chromatography matrix-assisted laser desorption/ io ... | 0 | 20167014 |
| big data: the future of biocuration. | 0 | 18769432 | |
| mechanisms and dynamics of orphan gene emergence in insect genomes. | orphan genes are defined as genes that lack detectable similarity to genes in other species and therefore no clear signals of common descent (i.e., homology) can be inferred. orphans are an enigmatic portion of the genome because their origin and function are mostly unknown and they typically make up 10% to 30% of all genes in a genome. several case studies demonstrated that orphans can contribute to lineage-specific adaptation. here, we study orphan genes by comparing 30 arthropod genomes, focu ... | 0 | 23348040 |
| a visible dominant marker for insect transgenesis. | transgenesis of most insects currently relies on fluorescence markers. here we establish a transformation marker system causing phenotypes visible to the naked eye due to changes in the color of melanin pigments, which are widespread in animals. ubiquitous overexpression of arylalkylamine-n-acetyl transferase in the silkworm, bombyx mori, changes the color of newly hatched first-instar larvae from black to a distinctive light brown color, and can be used as a molecular marker by directly connect ... | 0 | 23250425 |
| sub-lethal metal stress response of larvae of aedes aegypti. | aedes aegypti (diptera: culicidae) has adapted to urban environments; the urbanisation process provides suitable habitats for this disease vector subsequently increasing the probability of the transmission of pathogens in high-density environments. urban environments provide metal stressed larval habitats. however, little is known about the physiological cost of metal stress or how this might affect the performance of this mosquito species. this study aims to characterise the sub-lethal physiolo ... | 0 | 24926118 |
| molecular determinants of odorant receptor function in insects. | the olfactory system of drosophila melanogaster provides a powerful model to study molecular and cellular mechanisms underlying function of a sensory system. in the 1970s siddiqi and colleagues pioneered the application of genetics to olfactory research and isolated several mutant drosophila with odorant-specific defects in olfactory behaviour, suggesting that odorants are detected differentially by the olfactory system. since then basic principles of olfactory system function and development ha ... | 0 | 25116610 |
| bacillus thuringiensis cry1a toxins are versatile proteins with multiple modes of action: two distinct pre-pores are involved in toxicity. | cry proteins from bacillus thuringiensis are insecticidal pfts (pore-forming toxins). in the present study, we show that two distinct functional pre-pores of cry1ab are formed after binding of the protoxin or the protease-activated toxin to the cadherin receptor, but before membrane insertion. both pre-pores actively induce pore formation, although with different characteristics, and contribute to the insecticidal activity. we also analysed the oligomerization of the mutant cry1abmod protein. th ... | 0 | 24456341 |
| clinical, virologic, and epidemiologic characteristics of dengue outbreak, dar es salaam, tanzania, 2014. | we investigated a dengue outbreak in dar es salaam, tanzania, in 2014, that was caused by dengue virus (denv) serotype 2. denv infection was present in 101 (20.9%) of 483 patients. patient age and location of residence were associated with infection. seven (4.0%) of 176 patients were co-infected with malaria and denv. | 0 | 27088845 |
| an operational framework for insecticide resistance management planning. | arthropod vectors transmit organisms that cause many emerging and reemerging diseases, and their control is reliant mainly on the use of chemical insecticides. only a few classes of insecticides are available for public health use, and the increased spread of insecticide resistance is a major threat to sustainable disease control. the primary strategy for mitigating the detrimental effects of insecticide resistance is the development of an insecticide resistance management plan. however, few exa ... | 0 | 27089119 |
| seasonality and the effectiveness of mass vaccination. | many infectious diseases have seasonal outbreaks, which may be driven by cyclical environmental conditions (e.g., an annual rainy season) or human behavior (e.g., school calendars or seasonal migration). if a pathogen is only transmissible for a limited period of time each year, then seasonal outbreaks could infect fewer individuals than expected given the pathogen's in-season transmissibility. influenza, with its short serial interval and long season, probably spreads throughout a population un ... | 0 | 27105983 |
| detection of all four dengue serotypes in aedes aegypti female mosquitoes collected in a rural area in colombia. | the aedes aegypti vector for dengue virus (denv) has been reported in urban and periurban areas. the information about denv circulation in mosquitoes in colombian rural areas is limited, so we aimed to evaluate the presence of denv in ae. aegypti females caught in rural locations of two colombian municipalities, anapoima and la mesa. mosquitoes from 497 rural households in 44 different rural settlements were collected. pools of about 20 ae. aegypti females were processed for denv serotype detect ... | 0 | 27074252 |
| vaccinating in disease-free regions: a vaccine model with application to yellow fever. | concerns regarding natural or induced emergence of infectious diseases have raised a debate on the pros and cons of pre-emptive vaccination of populations under uncertain risk. in the absence of immediate risk, ethical issues arise because even smaller risks associated with the vaccine are greater than the immediate disease risk (which is zero). the model proposed here seeks to formalize the vaccination decision process looking from the perspective of the susceptible individual, and results are ... | 0 | 17442650 |
| climate change and developing-country cities: implications for environmental health and equity. | climate change is an emerging threat to global public health. it is also highly inequitable, as the greatest risks are to the poorest populations, who have contributed least to greenhouse gas (ghg) emissions. the rapid economic development and the concurrent urbanization of poorer countries mean that developing-country cities will be both vulnerable to health hazards from climate change and, simultaneously, an increasing contributor to the problem. we review the specific health vulnerabilities o ... | 0 | 17393341 |
| fever and prominent arthralgia in a traveler who returned from amazonia: differentiating between dengue fever and chikungunya fever. | 0 | 26157592 | |
| mal de mayo. | 0 | 17479908 | |
| biochemical mechanisms leading to tryptophan 2,3-dioxygenase activation. | tryptophan 2,3-dioxygenase (tdo) is the first enzyme in the tryptophan oxidation pathway. it is a hemoprotein and its heme prosthetic group is present as a heme-ferric (heme-fe(3+)) form that is not active. to be able to oxidize tryptophan, the heme-fe(3+) form of the enzyme must be reduced to a heme-ferrous (heme-fe(2+)) form and this study describes conditions that promote tdo activation. tdo is progressively activated upon mixing with tryptophan in a neutral buffer, which leads to an impressi ... | 0 | 17212352 |
| spatial vulnerability to dengue in a brazilian urban area during a 7-year surveillance. | this study considers the vulnerability of the urban area of the city of belo horizonte to dengue. a total number of 89,607 cases registered in the surveillance system from 1996 to 2002 were analyzed. seven epidemic waves were identified during this period. cases were grouped into 2,563 census areas, and three risk categories were proposed based on how many times each area reached a threshold established for each epidemic wave. the association between the risk categories and the socioeconomic, de ... | 0 | 17243024 |
| recent advances in dengue: relevance to puerto rico. | dengue represents an increasingly important public health challenge in puerto rico, with recent epidemics in 2007, 2010, and 2012-2013. although recent advances in dengue vaccine development offer hope for primary prevention, the role of health professionals in the diagnosis and management of dengue patients is paramount. case definitions for dengue, dengue with warning signs, and severe dengue provide a framework to guide clinical decision-making. furthermore, the differentiation between dengue ... | 0 | 26061055 |
| dual lipolytic control of body fat storage and mobilization in drosophila. | energy homeostasis is a fundamental property of animal life, providing a genetically fixed balance between fat storage and mobilization. the importance of body fat regulation is emphasized by dysfunctions resulting in obesity and lipodystrophy in humans. packaging of storage fat in intracellular lipid droplets, and the various molecules and mechanisms guiding storage-fat mobilization, are conserved between mammals and insects. we generated a drosophila mutant lacking the receptor (akhr) of the a ... | 0 | 17488184 |
| definition of the minimal fragments of sti1 required for dimerization, interaction with hsp70 and hsp90 and in vivo functions. | the molecular chaperone hsp (heat-shock protein) 90 is critical for the activity of diverse cellular client proteins. in a current model, client proteins are transferred from hsp70 to hsp90 in a process mediated by the co-chaperone sti1/hop, which may simultaneously interact with hsp70 and hsp90 via separate tpr (tetratricopeptide repeat) domains, but the mechanism and in vivo importance of this function is unclear. in the present study, we used truncated forms of sti1 to determine the minimal r ... | 0 | 17300223 |
| sexually dimorphic body size and development time plasticity in aedes mosquitoes (diptera: culicidae). | sexual size dimorphism (ssd) in insects often accompanies a sexual difference in development time, sexual bimaturism (sbm). | 0 | 25663826 |
| countermeasure development for rift valley fever: deletion, modification or targeting of major virulence factor nss. | rift valley fever (rvf) is a mosquito-borne zoonotic disease characterized by a high rate of abortion in ruminants, and febrile illness, hemorrhagic fever, retinitis and encephalitis in humans. rvf is caused by the rvf virus (rvfv), belonging to the genus phlebovirus of the family bunyaviridae. rvfv encodes a major virulence factor, nss, which is dispensable for viral replication, yet required for evasion of host innate immune responses. rvfv nss inhibits host gene upregulation at the transcript ... | 0 | 24910709 |
| the basic rules and methods of mosquito rearing (aedes aegypti). | the rearing of aedes mosquitoes is complex and demanding for several reasons. aedes larvae are affected by temperature, density and available nutrition, mating is not necessarily accomplished naturally and females need a blood meal to develop eggs. the climate chambers where the mosquitoes are kept are warm and sweaty. due to these tropical conditions the larvae develop fast and need to be cared for daily. the laboratory of entomology in national institute of malaria research bangalore has cultu ... | 0 | 24754030 |
| phytopharmacological overview of tribulus terrestris. | tribulus terrestris (family zygophyllaceae), commonly known as gokshur or gokharu or puncture vine, has been used for a long time in both the indian and chinese systems of medicine for treatment of various kinds of diseases. its various parts contain a variety of chemical constituents which are medicinally important, such as flavonoids, flavonol glycosides, steroidal saponins, and alkaloids. it has diuretic, aphrodisiac, antiurolithic, immunomodulatory, antidiabetic, absorption enhancing, hypoli ... | 0 | 24600195 |
| risk factors associated with death in brazilian children with severe dengue: a case-control study. | the purpose of this case-control study was to evaluate risk factors associated with death in children with severe dengue. | 0 | 24473560 |
| larvicidal efficacies and chemical composition of essential oils of pinus sylvestris and syzygium aromaticum against mosquitoes. | to assess the chemical composition and mosquito larvicidal potentials of essential oils of locally sourced pinus sylvestris (p. sylvestris) and syzygium aromaticum (s. aromaticum) against aedes aegypti (a. aegypti) and culex quinquefasciatus (c. quinquefasciatus). | 0 | 24144127 |
| close encounters: contributions of carbon dioxide and human skin odour to finding and landing on a host in aedes aegypti. | in a wind-tunnel study, the upwind flight and source location of female aedes aegypti to plumes of carbon dioxide (co2) gas and odour from human feet is tested. both odour sources are presented singly and in combination. flight upwind along the plumes is evident for both co2 and odour from human feet when the odours are presented alone. likewise, both odour sources are located by more than 70% of mosquitoes in less than 3 min. when both co2 and odour from human feet are presented simultaneously ... | 0 | 24839345 |