Publications

antibacterial and antifungal activities of various solvent extracts from the leaves and stem bark of anisophyllea laurina r. br ex sabine used as traditional medicine in guinea.	anisophyllea laurina r. br ex sabine is a plant that has been used in the folk medicine to treat malaria, dysentery, diabetes and toothache against bacterial infection. through this study, most likely we are reporting for the first time, its effectiveness as an antibacterial and antifungal agent.	2015	25843022
in silico and proteomic analysis of protein methyltransferase cher from bacillus subtilis.	protein methyltransferase (cher) catalyzes the methylation of the cytosolic domain of the membrane bound chemotaxis receptors, and plays a pivotal role in the chemotactic signal transduction pathway in bacteria. crystal structure of cher is available only from the gram-negative bacterium salmonella typhimurium (stcher), which contain a catalytic c-terminal domain, encompassing a β-subdomain, connected via a linker to the n-terminal domain. the structural-functional similitude between cher of the ...	2015	25799883
empirical prediction and validation of antibacterial inhibitory effects of various plant essential oils on common pathogenic bacteria.	in this study, fractional compound composition, antioxidant capacity, and phenolic substance content of 14 plant essential oils-anise (pimpinella anisum), bay leaves (laurus nobilis), cinnamon bark (cinnamomum verum), clove (eugenia caryophyllata), fennel (foeniculum vulgare), hop (humulus lupulus), istanbul oregano (origanum vulgare subsp. hirtum), izmir oregano (origanum onites), mint (mentha piperita), myrtus (myrtus communis), orange peel (citrus sinensis), sage (salvia officinalis), thyme ( ...	2015	25764982
investigation of the utility of complementary electrochemical detection techniques to examine the in vitro affinity of bacterial flagellins for a toll-like receptor 5 biosensor.	an initial investigation of the fabrication of a novel biosensor utilizing toll-like receptor 5 (tlr5) has been conducted. the detection assay using this sensor platform has been carried out using two complementary electrochemical techniques. the electrochemical properties of the modified bare gold surface following tlr5 immobilization were characterized. the electrochemical response to changes in the sensor film resistance and electron charge-transfer permittivity triggered by independent expos ...	2015	25709059
oxidative stress and antibacterial properties of a graphene oxide-cystamine nanohybrid.	oxidative stress can damage proteins, dna, and lipids, and is involved in the progression of many diseases. damage to infected cells caused by oxidative stress is related to increased levels of reactive oxygen species, including hydrogen peroxide. during oxidative stress, hydrogen peroxide levels are often increased and catalase level decreased inside cells. this can lead to the death of skin and other cells. hydrophobic low molecular weight compounds are useful in treating hemorrhagic condition ...	2015	25609960
the effects of the supplementation of bacillus subtilis rx7 and b2a strains on the performance, blood profiles, intestinal salmonella concentration, noxious gas emission, organ weight and breast meat quality of broiler challenged with salmonella typhimurium.	an experiment was conducted to evaluate the effects of b. subtilis rx7 and b. subtilis b2a on growth performance, blood profiles, intestinal salmonella population, noxious gas emission, organ weight and breast meat quality of broilers under s. typhimurium challenge. a total of 120, one-day-old ross 308 male broiler chicks were assigned to four dietary treatments, composed of six replications, with five birds per replication, for 10 day. the dietary treatment groups were negative control (nc; no ...	2015	25244020
in vitro toxicological assessment of iron oxide, aluminium oxide and copper nanoparticles in prokaryotic and eukaryotic cell types.	metallic nanoparticles (nps) have a variety of applications in different industries including pharmaceutical industry where these nps are used mainly for image analysis and drug delivery. the increasing interest in nanotechnology is largely associated with undefined risks to the human health and to the environment. therefore, in the present study cytotoxic and genotoxic effects of iron oxide, aluminium oxide and copper nanoparticles were evaluated using most commonly used assays i.e. ames assay, ...	2015	24896217
microbiological toxicity of tilmicosin on human colonic microflora in chemostats.	to evaluate the microbiological safety of tilmicosin on human intestinal microflora, four chemostat models of healthy human colonic ecosystems were exposed to tilmicosin (0, 0.436, 4.36, and 43.6 μg/ml) for 7 days. prior to and during drug exposure, three microbiological endpoints were monitored daily including short-chain fatty acids, bacterial counts and macrolide susceptibility. colonization resistance of each community was determined by 3 successive daily challenges of salmonella typhimurium ...	2015	26190303
antibacterial activity of the venom of heterometrus xanthopus.	heterometrus xanthopus (scorpion) is one of the most venomous and ancient arthropods. its venom contains anti-microbial peptides like hadrurin, scorpine, pandinin 1, and pandinin 2 that are able to effectively kill multidrug-resistant pathogens. the present study was conducted to evaluate the anti-bacterial activity of h. xanthopus venom. six gram-positive and gram-negative bacterial strains were tested against 1/100, 1/10, and 1/1 fractions of distilled water diluted and crude venom. 1/100 and ...	2015	23087515
three-dimensional tumor spheroids for in vitro analysis of bacteria as gene delivery vectors in tumor therapy.	several studies in animal models demonstrated that obligate and facultative anaerobic bacteria of the genera bifidobacterium, salmonella, or clostridium specifically colonize solid tumors. consequently, these and other bacteria are discussed as live vectors to deliver therapeutic genes to inhibit tumor growth. therapeutic approaches for cancer treatment using anaerobic bacteria have been investigated in different mouse models. in the present study, solid three-dimensional (3d) multicellular tumo ...	2015	26655167
disease tolerance mediated by microbiome e. coli involves inflammasome and igf-1 signaling.	infections and inflammation can lead to cachexia and wasting of skeletal muscle and fat tissue by as yet poorly understood mechanisms. we observed that gut colonization of mice by a strain of escherichia coli prevents wasting triggered by infections or physical damage to the intestine. during intestinal infection with the pathogen salmonella typhimurium or pneumonic infection with burkholderia thailandensis, the presence of this e. coli did not alter changes in host metabolism, caloric uptake, o ...	2015	26516283
caspase-11 cleaves gasdermin d for non-canonical inflammasome signalling.	intracellular lipopolysaccharide from gram-negative bacteria including escherichia coli, salmonella typhimurium, shigella flexneri, and burkholderia thailandensis activates mouse caspase-11, causing pyroptotic cell death, interleukin-1β processing, and lethal septic shock. how caspase-11 executes these downstream signalling events is largely unknown. here we show that gasdermin d is essential for caspase-11-dependent pyroptosis and interleukin-1β maturation. a forward genetic screen with ethyl-n ...	2015	26375259
burkholderia cenocepacia and salmonella enterica arnt proteins that transfer 4-amino-4-deoxy-l-arabinose to lipopolysaccharide share membrane topology and functional amino acids.	we recently demonstrated that incorporation of 4-amino-4-deoxy-l-arabinose (l-ara4n) to the lipid a moiety of lipopolysaccharide (lps) is required for transport of lps to the outer membrane and viability of the gram-negative bacterium burkholderia cenocepacia. arnt is a membrane protein catalyzing the transfer of l-ara4n to the lps molecule at the periplasmic face of the inner membrane, but its topology and mechanism of action are not well characterized. here, we elucidate the topology of arnt a ...	2015	26030265
microarray on digital versatile disc for identification and genotyping of salmonella and campylobacter in meat products.	highly portable, cost-effective, and rapid-response devices are required for the subtyping of the most frequent food-borne bacteria; thereby the sample rejection strategies and hygienization techniques along the food chain can be tailor-designed. here, a novel biosensor is presented for the generic detection of salmonella and campylobacter and the discrimination between their most prevalent serovars (salmonella enteritidis, salmonella typhimurium) and species (campylobacter jejuni, campylobacter ...	2015	26198111
microbiological survey of birds of prey pellets.	a microbiological survey of 73 pellets collected from different birds of prey species housed at the wildlife rescue and rehabilitation center of napoli (southern italy) was performed. pellets were analyzed by culture and biochemical methods as well as by serotyping and polymerase chain reaction. we isolated a wide range of bacteria some of them also pathogens for humans (i.e. salmonella enterica serotype typhimurium, campylobacter coli, escherichia coli o serogroups). this study highlights the p ...	2015	26026881
antimicrobial activity of customary medicinal plants of the yaegl aboriginal community of northern new south wales, australia: a preliminary study.	this study is a collaboration between macquarie university researchers and the yaegl aboriginal community of northern nsw, australia to investigate the antimicrobial potential of plants used in the topical treatment of wounds, sores and skin infections. based on previously documented medicinal applications, aqueous and aqueous ethanolic extracts of alocasia brisbanensis, canavalia rosea, corymbia intermedia, hibbertia scandens, ipomoea brasiliensis, lophostemon suaveolens and syncarpia glomulife ...	2015	26122212
prenylated polyphenolic compounds from glycyrrhiza iconica and their antimicrobial and antioxidant activities.	a new prenylated isoflavan, iconisoflavan (1), and a new prenylated isoflav-3-ene, iconisoflaven (2) were isolated from the roots of glycyrrhiza iconica together with four known ones namely (3s)-licoricidin (3), licorisoflavan a (4), topazolin (5) and glycycoumarin (6). the structures were elucidated on the basis of extensive spectroscopic analysis including 1d and 2d nmr as well as hr-ms. furthermore, the absolute configurations of compounds 1, 3 and 4 were established by electronic circular di ...	2015	25963162
bioactive endophytic fungi isolated from caesalpinia echinata lam. (brazilwood) and identification of beauvericin as a trypanocidal metabolite from fusarium sp.	aiming to identify new sources of bioactive secondary metabolites, we isolated 82 endophytic fungi from stems and barks of the native brazilian tree caesalpinia echinata lam. (fabaceae). we tested their ethyl acetate extracts in several in vitro assays. the organic extracts from three isolates showed antibacterial activity against staphylococcus aureus and escherichia coli [minimal inhibitory concentration (mic) 32-64 μg/ml]. one isolate inhibited the growth of salmonella typhimurium (mic 64 μg/ ...	2015	25606862
isolation and taxonomic identity of bacteriocin-producing lactic acid bacteria from retail foods and animal sources.	bacteriocin-producing (bac⁺) lactic acid bacteria (lab) were isolated from a variety of food products and animal sources. samples were enriched in de man, rogosa, and sharpe (mrs) lactocilli broth and plated onto mrs agar plates using a "sandwich overlay" technique. inhibitory activity was detected by the "deferred antagonism" indicator overlay method using listeria monocytogenes as the primary indicator organism. antimicrobial activity against l. monocytogenes was detected by 41 isolates obtain ...	2015	27682080
statistical approaches to developing a multiplex immunoassay for determining human exposure to environmental pathogens.	there are numerous pathogens that can be transmitted through water. identifying and understanding the routes and magnitude of exposure or infection to these microbial contaminants are critical to assessing and mitigating risk. conventional approaches of studying immunological responses to exposure or infection such as enzyme-linked immunosorbent assays (elisas) and other monoplex antibody-based immunoassays can be very costly, laborious, and consume large quantities of patient sample. a major li ...	2015	26070441
inhibition of adhesion of intestinal pathogens (escherichia coli, vibrio cholerae, campylobacter jejuni, and salmonella typhimurium) by common oligosaccharides.	inhibition of the binding of pathogenic adhesins to host glycans by suitable oligosaccharides forms the basis of antiadhesion therapies. experiments were carried out to study the inhibition capability of oligosaccharides on the adhesion of four microorganisms (escherichia coli, vibrio cholerae, campylobacter jejuni, and salmonella typhimurium) to ht-29 cells. results showed that, in the absence of oligosaccharides, all of the four pathogens efficiently adhered to the cells. cell adhesion with di ...	2015	25692734
administration of defined microbiota is protective in a murine salmonella infection model.	salmonella typhimurium is a major cause of diarrhea and causes significant morbidity and mortality worldwide, and perturbations of the gut microbiota are known to increase susceptibility to enteric infections. the purpose of this study was to investigate whether a microbial ecosystem therapeutic (met-1) consisting of 33 bacterial strains, isolated from human stool and previously used to cure patients with recurrent clostridium difficile infection, could also protect against s. typhimurium diseas ...	2015	26531327
a highly acid-resistant novel strain of lactobacillus johnsonii no. 1088 has antibacterial activity, including that against helicobacter pylori, and inhibits gastrin-mediated acid production in mice.	a novel strain of lactobacillus johnsonii no. 1088 was isolated from the gastric juice of a healthy japanese male volunteer, and characterized for its effectiveness in the stomach environment. lactobacillus johnsonii no. 1088 was found to have the strongest acid resistance among several lactobacilli examined (>10% of cells survived at ph 1.0 after 2 h), and such a high acid resistance property was a specific characteristic of this strain of l. johnsonii. when cultured with various virulent bacte ...	2015	25771812
low dietary iron intake restrains the intestinal inflammatory response and pathology of enteric infection by food-borne bacterial pathogens.	orally administrated iron is suspected to increase susceptibility to enteric infections among children in infection endemic regions. here we investigated the effect of dietary iron on the pathology and local immune responses in intestinal infection models. mice were held on iron-deficient, normal iron, or high iron diets and after 2 weeks they were orally challenged with the pathogen citrobacter rodentium. microbiome analysis by pyrosequencing revealed profound iron- and infection-induced shifts ...	2015	26046550
crystal structure of dsba from corynebacterium diphtheriae and its functional implications for cuep in gram-positive bacteria.	in gram-negative bacteria in the periplasmic space, the dimeric thioredoxin-fold protein dsbc isomerizes and reduces incorrect disulfide bonds of unfolded proteins, while the monomeric thioredoxin-fold protein dsba introduces disulfide bonds in folding proteins. in the gram-negative bacteria salmonella enterica serovar typhimurium, the reduced form of cuep scavenges the production of hydroxyl radicals in the copper-mediated fenton reaction, and dsbc is responsible for keeping cuep in the reduced ...	2015	26082031
first detection of macrorhabdus ornithogaster in wild eurasian siskins (carduelis spinus) in germany. a case study.	the colonization of the gastric ascomycetous yeast macrorhabdus (m.) ornithogaster could be associated with a chronic wasting disease in several bird species in captivity. the prevalence and clinical relevance of m. ornithogaster in wild birds is unknown in detail.	2015	25804259
in vitro probiotic characteristics of lactobacillus plantarum zdy 2013 and its modulatory effect on gut microbiota of mice.	lactobacillus plantarum zdy 2013, a novel strain isolated from chinese traditional fermented acid beans, was systematically evaluated for its survival capacity under stress conditions (ph, bile salt, simulated gastrointestinal tract, and antibiotics), production of exopolysaccharide and antagonism against 8 pathogens. its effect on mice gut microbiota was also investigated by quantitative pcr and pcr-denaturing gradient gel electrophoresis. the results showed that zdy 2013 can grow at ph 3.5 and ...	2015	26142853
loss of the lac operon contributes to salmonella invasion of epithelial cells through derepression of flagellar synthesis.	salmonella, a genus that is closely related to escherichia coli, includes many pathogens of humans and other animals. a notable feature that distinguishes salmonella from e. coli is lactose negativity, because the lac operon is lost in most salmonella genomes. here, we expressed the lac operon in salmonella enterica serovar typhimurium and compared the virulence of the lac(+) strain to that of the wild-type strain in a murine model, invasion assays, and macrophage replication assays. we showed t ...	2015	25362512
deciphering the role of the type ii glyoxalase isoenzyme ycbl (glxii-2) in escherichia coli.	in escherichia coli, detoxification of methylglyoxal (mg) requires glyoxalases i and ii. glyoxalase i (gloa/glxi) isomerizes the hemithioacetal, formed spontaneously from mg and glutathione (gsh) to s-lactoylglutathione (slg), which is hydrolyzed by glyoxalase ii (glob/glxii) to lactate and gsh. ycbl from salmonella enterica serovar typhimurium is an unusual type ii glyoxalase whose role in mg detoxification has remained enigmatic. here we show that ycbl (gloc/glxii-2) acts as an accessory type ...	2015	25670698
genotoxicity studies on the root extract of polygala tenuifolia willdenow.	the root of polygala tenuifolia willdenow has been used for the treatment against insomnia, amnesia, depression, palpitations with anxiety, and memory improvement. however, there is no sufficient background information on toxicological evaluation of the root to given an assurance of safety for developing dietary supplements and functional foods. as part of a safety evaluation, the potential genotoxicity of the root extract of p. tenuifolia was evaluated using a standard battery of tests (bacteri ...	2015	25666111
evaluation of antimicrobial activities of sequential spray applications of decontamination treatments on chicken carcasses.	the objective of this study was to evaluate the effects of sequential applications of ɛ-polylysine (epl) or lauramide arginine ethyl ester (lae) sprays followed by an acidic calcium sulfate (acs) spray on inoculated chicken carcasses to reduce salmonella (salmonella enterica serovars including salmonella typhimurium and salmonella enteritidis) contamination during 6 days of storage (4.4°c). secondly, reductions of the resident microflora were studied on uninoculated chicken carcasses following t ...	2015	25656180
perforin-2 is essential for intracellular defense of parenchymal cells and phagocytes against pathogenic bacteria.	perforin-2 (mpeg1) is a pore-forming, antibacterial protein with broad-spectrum activity. perforin-2 is expressed constitutively in phagocytes and inducibly in parenchymal, tissue-forming cells. in vitro, perforin-2 prevents the intracellular replication and proliferation of bacterial pathogens in these cells. perforin-2 knockout mice are unable to control the systemic dissemination of methicillin-resistant staphylococcus aureus (mrsa) or salmonella typhimurium and perish shortly after epicutane ...	2015	26402460
rosa canina l.--new possibilities for an old medicinal herb.	health beneficial properties of rosa canina species are mainly attributed to rose hips, while the leaves are usually discarded as waste. in the present study we investigated chemical constituents as well as antimicrobial and antibiofilm potential of r. canina methanolic leaf extract. chemical analysis showed that dominant phenolic compounds are quercetin and isorhamnetin derivatives (isoquercetin and isorhamnetin-3-o-rutinoside). among the tested bacteria, pseudomonas aeruginosa and salmonella t ...	2015	26399901
chip-seq analysis of the σe regulon of salmonella enterica serovar typhimurium reveals new genes implicated in heat shock and oxidative stress response.	the alternative sigma factor σe functions to maintain bacterial homeostasis and membrane integrity in response to extracytoplasmic stress by regulating thousands of genes both directly and indirectly. the transcriptional regulatory network governed by σe in salmonella and e. coli has been examined using microarray, however a genome-wide analysis of σe-binding sites in salmonella has not yet been reported. we infected macrophages with salmonella typhimurium over a select time course. using chroma ...	2015	26389830
ceramide and sphingosine in pulmonary infections.	acid sphingomyelinase and ceramide have previously been shown to play a central role in infections with neisseria gonorrhoeae, staphylococcus aureus, listeria monocytogenes, pseudomonas aeruginosa, salmonella typhimurium, escherichia coli, and mycobacterium avium. recent studies have extended the role of sphingolipids in bacterial infections and have demonstrated that ceramide and sphingosine are central to the defense of lungs against bacterial pathogens. ceramide accumulates in the airway epit ...	2015	25720061
presence and correlation of some enteric indicator bacteria, diarrheagenic escherichia coli pathotypes, and salmonella serotypes in alfalfa sprouts from local retail markets in pachuca, mexico.	data on the presence of diarrheagenic escherichia coli pathotypes (deps) in alfalfa sprouts and correlations between the presence of coliform bacteria (cb), fecal coliforms (fc), e. coli, deps, and salmonella in alfalfa sprouts are not available. the presence of and correlations between cb, fc, e. coli, deps, and salmonella in alfalfa sprouts were determined. one hundred sprout samples were collected from retail markets in pachuca, hidalgo state, mexico. the presence of indicator bacteria and sa ...	2015	25719889
first two domains at the lp_1643 protein n terminus inhibit pathogen adhesion to porcine mucus in vitro.	gastrointestinal probiotics are important members of intestinal microflora in both healthy animals and human beings, and these bacteria may reduce the risk of infection caused by certain opportunistic pathogens through exclusive inhibition, competition, and displacement. the lp_1643 protein on the cell surface of lactobacillus plantarum wcfsi was assumed to possess a mucus-binding capability. this study aimed to determine if purified his-n2 protein exclusively inhibits pathogen adhesion to porci ...	2015	25710153
(aminophosphane)gold(i) and silver(i) complexes as antibacterial agents.	this manuscript describes the synthesis of new au(i) and ag(i) complexes with aminophosphane ligands and a study of their antibacterial activity against gram-negative salmonella enterica serovar typhimurium and escherichia coli and gram-positive listeria monocytogenes and staphylococcus aureus. the bactericidal assays revealed the effectiveness of these compounds on paradigm gram-negative and gram-positive pathogens, showing a moderate antimicrobial activity, comparable with the antibiotics of r ...	2015	25706322
evaluation of dna binding, radicals scavenging and antimicrobial studies of newly synthesized n-substituted naphthalimides: spectroscopic and molecular docking investigations.	in this study, we investigated a new series of naphthalimide based schiff base compounds as potential dna binding, antioxidant and antimicrobial agents. the structural characterization of synthesized compounds was carried out with the aid of elemental analysis and spectroscopic techniques (uv-vis., ir, (1)h and (13)c nmr). the dna binding properties of target compounds against ct-dna (calf thymus) have been investigated in detail by numerous biophysical techniques (uv-vis, fluorescence, ethidium ...	2015	26462815
model-driven discovery of synergistic inhibitors against e. coli and s. enterica serovar typhimurium targeting a novel synthetic lethal pair, alda and prpc.	mathematical models of biochemical networks form a cornerstone of bacterial systems biology. inconsistencies between simulation output and experimental data point to gaps in knowledge about the fundamental biology of the organism. one such inconsistency centers on the gene alda in escherichia coli: it is essential in a computational model of e. coli metabolism, but experimentally it is not. here, we reconcile this disparity by providing evidence that alda and prpc form a synthetic lethal pair, a ...	2015	26441892
transfer of the cloned salmonella spi-1 type iii secretion system and characterization of its expression mechanisms in gram negative bacteria in comparison with cloned spi-2.	cloned type iii secretion systems have much potential to be used for bacterial engineering purposes involving protein secretion and substrate translocation directly into eukaryotic cells. we have previously cloned the spi-1 and spi-2 type iii systems from the salmonella enterica serovar typhimurium genome using plasmid r995 which can conveniently capture large genomic segments for transfer between bacterial strains. however, though expressed and functional in salmonella strains, cloned spi-1 was ...	2015	26505312
activation of inflammasome by attenuated salmonella typhimurium in bacteria-mediated cancer therapy.	escherichia coli and attenuated salmonella both naturally accumulate in a tumor mass, yet have distinct therapeutic efficacy: the e. coli k-12 strain (mg1655) cannot induce as significant a tumor suppression as attenuated salmonella typhimurium, despite similar levels of accumulation in the tumor. to elucidate the mechanism of the robust antitumor effect of s. typhimurium, the cytokine profiles elicited by bacterial colonization in tumors were analyzed. c57bl/6 mice bearing mc38 tumors were inje ...	2015	26500022
salmonella typhimurium suppresses tumor growth via the pro-inflammatory cytokine interleukin-1β.	although strains of attenuated salmonella typhimurium and wild-type escherichia coli show similar tumor-targeting capacities, only s. typhimurium significantly suppresses tumor growth in mice. the aim of the present study was to examine bacteria-mediated immune responses by conducting comparative analyses of the cytokine profiles and immune cell populations within tumor tissues colonized by e. coli or attenuated salmonellae. ct26 tumor-bearing mice were treated with two different bacterial strai ...	2015	26516371
uv-heat treatments for the control of foodborne microbial pathogens in chicken broth.	this investigation established the process criteria for using uv-c light and mild heat (uv-h treatment) to inactivate 5-log10 cycles (performance criterion) of common foodborne pathogen populations, escherichia coli, salmonella typhimurium, listeria monocytogenes, and staphylococcus aureus, when inoculated in chicken broth. to define the target microorganism and the proper uv-h treatment conditions (including uv dose, treatment time, and temperature) that would achieve the stated performance cri ...	2015	26539493
[safety evaluation of rare sugar syrup: single-dose oral toxicity in rats, reverse mutation assay, chromosome aberration assay, and acute non-effect level for diarrhea of a single dose in humans].	the safety of rare sugar syrup obtained from high-fructose corn syrup under slightly alkaline conditions was studied. mutagenicity of rare sugar syrup was assessed by a reverse mutation assay using salmonella typhimurium and escherichia coli, and an in vitro chromosomal aberration assay using chinese hamster lung cell line (chl/iu). no mutagenicity of rare sugar syrup was detected under these experimental conditions. oral administration of single dose (15,000 mg/kg) of rare sugar syrup to rats c ...	2015	26537651
storage stability and antibacterial activity of eugenol nanoliposomes prepared by an ethanol injection-dynamic high-pressure microfluidization method.	eugenol is a major phenolic component with diverse biological activities. however, it is difficult to formulate into an aqueous solution due to poor water solubility, and this limits its application. in the present study, eugenol nanoliposomes (en) were prepared by combining the ethanol injection method with the dynamic high-pressure microfluidization method. good physicochemical characterizations of en were obtained. the successful encapsulation of eugenol in nanoliposomes was confirmed by four ...	2015	25581174
short communication: in vitro assessment of antioxidant, antibacterial and phytochemical analysis of peel of citrus sinensis.	antibacterial effect of citrus sinensis peel extracts was evaluated against several pathogenic bacteria associated with human and fish infections viz., escherichia coli, pseudomonas aeruginosa, klebsiella pneumonia, staphylococcus aureus, streptococcus pyogenes, staphylococcus epidermidis, serratia marcesnces, shigella flexneri, enterobacter amnigenus, salmonella typhimurium and serratia odorifera. methanol, ethanol, chloroform and diethyl ether solvents were used for extraction. in vitro antiba ...	2015	25553700
movement of salmonella serovar typhimurium and e. coli o157:h7 to ripe tomato fruit following various routes of contamination.	salmonella serovars have been associated with the majority of foodborne illness outbreaks involving tomatoes, and e. coli o157:h7 has caused outbreaks involving other fresh produce. contamination by both pathogens has been thought to originate from all points of the growing and distribution process. to determine if salmonella serovar typhimurium and e. coli o157:h7 could move to the mature tomato fruit of different tomato cultivars following contamination, three different contamination scenarios ...	2015	27682118
l-asparaginase delivered by salmonella typhimurium suppresses solid tumors.	bacteria can be engineered to deliver anticancer proteins to tumors via a controlled expression system that maximizes the concentration of the therapeutic agent in the tumor. l-asparaginase (l-asnase), which primarily converts asparagine to aspartate, is an anticancer protein used to treat acute lymphoblastic leukemia. in this study, salmonellae were engineered to express l-asnase selectively within tumor tissues using the inducible arabad promoter system of escherichia coli. antitumor efficacy ...	2015	27119104
characterization and bioactivities of an exopolysaccharide produced by lactobacillus plantarum yw32.	the exopolysaccharide (eps) produced by lactobacillus plantarum yw32 was purified and characterized, and the in vitro bioactivities of the purified eps were also evaluated. the eps had a molecular weight of 1.03×10(5) da, and it consisted of mannose, fructose, galactose and glucose in an approximate molar ratio of 8.2:1:4.1:4.2. microstructural studies of the eps demonstrated a web-like structure composed of compact ropes, and presence of many homogeneous rod-shaped lumps. the eps also showed hi ...	2015	25532782
evaluation of pathogen inactivation on sliced cheese induced by encapsulated atmospheric pressure dielectric barrier discharge plasma.	pathogen inactivation induced by atmospheric pressure dielectric barrier discharge (dbd) (250 w, 15 khz, air discharge) produced in a rectangular plastic container and the effect of post-treatment storage time on inactivation were evaluated using agar plates and cheese slices. when agar plates were treated with plasma, populations of escherichia coli, salmonella typhimurium, and listeria monocytogenes showed 3.57, 6.69, and 6.53 decimal reductions at 60 s, 45 s, and 7 min, respectively. when the ...	2015	25475265
effect of dietary supplementation of bacteriophage on performance, egg quality and caecal bacterial populations in laying hens.	1. bacteriophages (bp) have gained increasing attention as a treatment of bacterial infection for animals. however, the data pertaining to dietary application of bp for laying hens have been limited. 2. this study aimed to investigate the effect of dietary bp on laying performance, egg quality and caecal bacterial populations in laying hens. 3. the dietary bp used in this experiment was a mixture of individual bp targeting salmonella gallinarum, salmonella pullorum, salmonella typhimurium, salmo ...	2015	25471539
toll-like receptor 2 and nlrp3 cooperate to recognize a functional bacterial amyloid, curli.	amyloids are proteins with cross-β-sheet structure that contribute to pathology and inflammation in complex human diseases, including alzheimer's disease, parkinson's disease, type ii diabetes, and secondary amyloidosis. bacteria also produce amyloids as a component of their extracellular matrix during biofilm formation. recently, several human amyloids were shown to activate the nlrp3 inflammasome, leading to the activation of caspase 1 and production of interleukin 1β (il-1β). in this study, w ...	2015	25422268
utilization of plant-derived recombinant human β-defensins (hbd-1 and hbd-2) for averting salmonellosis.	we describe the use of plant-made β-defensins as effective antimicrobial substances for controlling salmonellosis, a deadly infection caused by salmonella typhimurium (referred to further as s. typhi). human β-defensin-1 (hbd-1) and -2 (hbd-2) were expressed under the control of strong constitutive promoters in tobacco plants, and bio-active β-defensins were successfully extracted. in the in vitro studies, enriched recombinant plant-derived human β-defensin-1 (phbd-1) and -2 (phbd-2) obtained fr ...	2015	25417183
microbial growth parameters obtained from the analysis of time to detection data using a novel rearrangement of the baranyi-roberts model.	to explore the predictions of a novel rearrangement of the baranyi-roberts model (brm) with time to detection data obtained from optical density data of microbial growth.	2015	25393511
characterization of salmonella typhimurium dna gyrase as a target of quinolones.	quinolones exhibit good antibacterial activity against salmonella spp. isolates and are often the choice of treatment for life-threatening salmonellosis due to multi-drug resistant strains. to assess the properties of quinolones, we performed an in vitro assay to study the antibacterial activities of quinolones against recombinant dna gyrase. we expressed the s. typhimurium dna gyrase a (gyra) and b (gyrb) subunits in escherichia coli. gyra and gyrb were obtained at high purity (>95%) by nickel- ...	2015	25381884
concentration and pattern changes of porcine serum apolipoprotein a-i in four different infectious diseases.	apolipoprotein a-i (apo a-i) is a major protein in lipid/lipoprotein metabolism and decreased serum levels have been observed in many species in response to inflammatory and infectious challenges. little is known about the porcine homologue, therefore in this work we have characterized it through biochemical and proteomic techniques. in 2de, porcine serum apo a-i is found as three spots, the two more acidic ones corresponding to the mature protein, the more basic spot to the protein precursor. d ...	2015	25377528
effect of detergents on galactoside binding by melibiose permeases.	the effect of various detergents on the stability and function of the melibiose permeases of escherichia coli (melbec) and salmonella typhimurium (melbst) was studied. in n-dodecyl-β-d-maltoside (ddm) or n-undecyl-β-d-maltoside (udm), wt melbst binds melibiose with an affinity similar to that in the membrane. however, with wt melbec or melbst mutants (arg141 → cys, arg295 → cys, or arg363 → cys), galactoside binding is not detected in these detergents, but binding to the phosphotransferase prote ...	2015	26352464
residue-dependent thermodynamic cost and barrel plasticity balances activity in the phopq-activated enzyme pagp of salmonella typhimurium.	pagp is an eight-stranded transmembrane β-barrel enzyme indispensable for lipid a palmitoylation in gram-negative bacteria. the severity of infection by pathogens, including salmonella, legionella, and bordetella, and resistance to antimicrobial peptides, relies on lipid a remodeling by pagp, rendering pagp a sought-after drug target. despite a conserved sequence, more robust palmitoylation of lipid a is observed in salmonella typhimurium compared to escherichia coli, a possible consequence of t ...	2015	26334694
protection of neonatal broiler chickens following in ovo delivery of oligodeoxynucleotides containing cpg motifs (cpg-odn) formulated with carbon nanotubes or liposomes.	unformulated oligodeoxynucleotides (odn) containing cpg motifs (cpg-odn) have been shown to stimulate the innate immune system against a variety of bacterial, viral, and protozoan infections in a variety of vertebrate species. we have previously shown that in ovo delivery of unformulated cpg-odn was able to significantly protect neonatal broiler chickens against escherichia coli or salmonella typhimurium infections. the objectives of this study were to examine the safety and immunoprotective eff ...	2015	26292531
an avian influenza a (h7n9) virus vaccine candidate based on the fusion protein of hemagglutinin globular head and salmonella typhimurium flagellin.	a novel influenza virus, subtype h7n9, circulated through china in 2013-2014. its higher rates of human infection in a wide range of locations within china and the associated increased likelihood of human-to-human transmission have caused global concern. recombinant subunit vaccines provide safe and targeted protection against viral infections. however, the protective efficacy of recombinant subunit vaccines tends to be less potent than vaccines made from whole viruses. studies have shown that b ...	2015	26286143
genotoxicity evaluation of oryeong-san water extract using in vitro and in vivo tests.	oryeong-san, a mixture of five herbal plants, is a well-known therapy for renal-associated diseases such as those manifesting edema, dysuria, and oliguria.	2015	26268991
heat-killed lactobacillus spp. cells enhance survivals of caenorhabditis elegans against salmonella and yersinia infections.	this study examined the effect of feeding heat-killed lactobacillus cells on the survival of caenorhabditis elegans nematodes after salmonella typhimurium and yersinia enterocolitica infection. the feeding of heat-killed lactobacillus plantarum 133 (lp133) and lactobacillus fermentum 21 (lp21) cells to nematodes was shown to significantly increase the survival rate as well as stimulate the expression of pmk-1 gene that key factor for c. elegans immunity upon infection compared with control nemat ...	2015	26250615
salmonella typhimurium strain expressing oprf-opri protects mice against fatal infection by pseudomonas aeruginosa.	pseudomonas aeruginosa poses a major threat to human health and to the mink industry. thus, development of vaccines that elicit robust humoral and cellular immunity against p. aeruginosa is greatly needed. in this study, a recombinant attenuated salmonella vaccine (rasv) that expresses the outer membrane proteins fusion oprf190-342 -opri21-83 (f1i2) from p. aeruginosa was constructed and the potency of this vaccine candidate assessed by measuring f1i2-specific humoral immune responses upon vacci ...	2015	26249788
inhibition of pathogenic bacterial growth on excision wound by green synthesized copper oxide nanoparticles leads to accelerated wound healing activity in wistar albino rats.	an impaired wound healing is one of the major health related problem in diabetic and non-diabetic patients around the globe. the pathogenic bacteria play a predominant role in delayed wound healing, owing to interaction in the wound area. in our previous work we developed green chemistry mediated copper oxide nanoparticles using ficus religiosa leaf extract. in the present study we make an attempt to evaluate the anti-bacterial, and wound healing activity of green synthesized copper oxide nanopa ...	2015	26194977
laminarin from irish brown seaweeds ascophyllum nodosum and laminaria hyperborea: ultrasound assisted extraction, characterization and bioactivity.	ultrasound assisted extraction (uae), purification, characterization and antioxidant activity of laminarin from irish brown seaweeds ascophyllum nodosum and laminarina hyperborea were investigated. uae was carried out using 60% ultrasonic power amplitude and 0.1 m hydrochloric acid for 15 min. separately, solid-liquid extraction was carried in an orbital shaker using 0.1 m hydrochloric acid at 70 °c for 2.5 h. uae with hydrochloric acid resulted in the highest concentration of laminarin, 5.82% a ...	2015	26184235
evaluation of a water extract of so-cheong-ryong-tang for acute toxicity and genotoxicity using in vitro and in vivo tests.	so-cheong-ryong-tang, a traditional korean medicine, has been used empirically for the treatment of asthma, allergic rhinitis, and colds for hundreds of years. however, its genotoxicity has been rarely examined.	2015	26178777
functional analysis of a novel cysteine-rich antimicrobial peptide from the salivary glands of the tick rhipicephalus haemaphysaloides.	ticks encounter various microbes while sucking blood from an infected host and carrying these pathogens in themselves. ticks can then transmit these pathogens to vertebrate hosts. the immune system of ticks can be stimulated to produce many bioactive molecules during feeding and pathogen invasion. antimicrobial peptides (amps) are key effector molecules of a tick's immune response, as they can kill invading pathogenic microorganisms. in this study, we identified a novel cysteine-rich amp, design ...	2015	26152423
quantitative distribution of salmonella spp. and escherichia coli on beef carcasses and raw beef at retail establishments.	salmonella is a foodborne pathogen that commonly inhabits the gastrointestinal tract of a healthy feedlot cattle and can be transferred to the carcass surface during hide removal and evisceration procedures. numerous investigations on salmonella prevalence throughout different stages of the beef chain have been conducted. in contrast, limited studies are available on quantitative determinations of salmonella at different steps in raw meat production. quantitative data, particularly for pathogeni ...	2015	26125489
inactivation of pathogenic bacteria inoculated onto a bacto™ agar model surface using tio2-uvc photocatalysis, uvc and chlorine treatments.	the aim of this study was to study inactivation of different pathogenic bacteria on agar model surface using tio2-uv photocatalysis (tuvp).	2015	26099167
salmonella typhimurium and escherichia coli dissimilarity: closely related bacteria with distinct metabolic profiles.	live attenuated strains of salmonella typhimurium have been extensively investigated as vaccines for a number of infectious diseases. however, there is still little information available concerning aspects of their metabolism. s. typhimurium and escherichia coli show a high degree of similarity in terms of their genome contents and metabolic networks. however, this work presents experimental evidence showing that significant differences exist in their abilities to direct carbon fluxes to biomass ...	2015	26097206
cold plasma inactivation of internalised bacteria and biofilms for salmonella enterica serovar typhimurium, listeria monocytogenes and escherichia coli.	microbial biofilms and bacteria internalised in produce tissue may reduce the effectiveness of decontamination methods. in this study, the inactivation efficacy of in-package atmospheric cold plasma (acp) afterglow was investigated against salmonella typhimurium, listeria monocytogenes and escherichia coli in the forms of planktonic cultures, biofilms formed on lettuce and associated bacteria internalised in lettuce tissue. prepared lettuce broth (3%) was inoculated with bacteria resulting in a ...	2015	26093991
effect of cell immobilization on the growth dynamics of salmonella typhimurium and escherichia coli at suboptimal temperatures.	predictive microbiology has recently acknowledged the impact of the solid(like) food structure on microbial behavior. the presence of this solid(like) structure causes microorganisms to grow as colonies and no longer planktonically as in liquid. in this paper, the growth dynamics of salmonella typhimurium and escherichia coli were studied as a function of temperature, considering different growth morphologies, i.e., (i) planktonic cells, (ii) immersed colonies and (iii) surface colonies. for all ...	2015	26057111
dissemination of inci2 plasmids that harbor the blactx-m element among clinical salmonella isolates.	the extended-spectrum-β-lactamase (esbl) determinant ctx-m-55 is increasingly prevalent in escherichia coli but remains extremely rare in salmonella. this study reports the isolation of a plasmid harboring the blactx-m-55 element in a clinical salmonella enterica serotype typhimurium strain resistant to multiple antibiotics. this plasmid is genetically identical to several known inci2-type elements harbored by e. coli strains recovered from animals. this finding indicates that inci2 plasmids har ...	2015	26014934
preliminary incidence and trends of infection with pathogens transmitted commonly through food - foodborne diseases active surveillance network, 10 u.s. sites, 2006-2014.	foodborne illnesses represent a substantial, yet largely preventable, health burden in the united states. in 10 u.s. geographic areas, the foodborne diseases active surveillance network (foodnet) monitors the incidence of laboratory-confirmed infections caused by nine pathogens transmitted commonly through food. this report summarizes preliminary 2014 data and describes changes in incidence compared with 2006-2008 and 2011-2013. in 2014, foodnet reported 19,542 infections, 4,445 hospitalizations ...	2015	25974634
control of pathogens in biofilms on the surface of stainless steel by levulinic acid plus sodium dodecyl sulfate.	the efficacy of levulinic acid (lva) plus sodium dodecyl sulfate (sds) to remove or inactivate listeria monocytogenes, salmonella typhimurium, and shiga toxin-producing escherichia coli (stec) in biofilms on the surface of stainless steel coupons was evaluated. five- or six-strain mixtures (ca. 9.0 log cfu/ml) of the three pathogens were separately inoculated on stainless steel coupons. after incubation at 21 °c for 72 h, the coupons were treated for 10 min by different concentrations of lva plu ...	2015	25950851
(1)h, (13)c and (15)n resonance assignments of σ(s) activating protein crl from salmonella enterica serovar typhimurium.	the general stress response in enterobacteria, like escherichia coli or salmonella, is controlled by the transcription factor σ(s), encoded by the rpos gene, which accumulates during stationary phase growth and associates with the core rna polymerase enzyme (e) to promote transcription of genes involved in cell survival. tight regulation of σ(s) is essential to preserve the balance between self-preservation under stress conditions and nutritional competence in the absence of stress. whereas σ fa ...	2015	25943268
the mutagenic assessment of mainstream cigarette smoke using the ames assay: a multi-strain approach.	salmonella typhimurium strains ta1535, ta1537, ta97, ta102 and ta104 were assessed for their suitability and use in conjunction with a vitrocell(®) vc 10 smoking robot and 3r4f reference mainstream cigarette smoke. little information exists on ta97, ta104, ta1535, ta1537 and ta102 using an aerosol 35mm spread-plate format. in this study, ta1535 and ta1537 were considered sub-optimal for use with a scaled-down format, due to low spontaneous revertant numbers (0-5 revertants/plate). in the context ...	2015	25868126
removal of pathogenic bacterial biofilms by combinations of oxidizing compounds.	bacterial biofilms are commonly formed on medical devices and food processing surfaces. the antimicrobials used have limited efficacy against the biofilms; therefore, new strategies to prevent and remove these structures are needed. here, the effectiveness of brief oxidative treatments, based on the combination of sodium hypochlorite (naclo) and hydrogen peroxide (h2o2) in the presence of copper sulfate (cuso4), were evaluated against bacterial laboratory strains and clinical isolates, both in p ...	2015	25864510
salmonella ghosts expressing enterotoxigenic escherichia coli k88ab, k88ac, k99, and fasa fimbrial antigens induce robust immune responses in a mouse model.	bacterial ghosts can be developed as safe and effective vaccines against bacterial infectious disease such as enterotoxigenic escherichia coli (etec)-induced diarrhea in neonatal piglets.	2015	25853619
production of poly(3-hydroxypropionate) and poly(3-hydroxybutyrate-co-3-hydroxypropionate) from glucose by engineering escherichia coli.	poly(3-hydroxypropionate) (p3hp) is the strongest family member of microbial polyhydroxyalkanoates (pha) synthesized by bacteria grown on 1,3-propandiol or glycerol. in this study synthesis pathways of p3hp and its copolymer p3hb3hp of 3-hydroxybutyrate (3hb) and 3-hydroxypropionate (3hp) were assembled respectively to allow their synthesis from glucose, a more abundant carbon source. recombinant escherichia coli was constructed harboring the p3hp synthetic pathway consisting of heterologous gen ...	2015	25842374
plectranthus amboinicus leaves stimulate growth of probiotic l. plantarum: evidence for ethnobotanical use in diarrhea.	leaves of plectranthus amboinicus are consumed in india along with buttermilk (a probiotic source) during pathogen induced diarrhea. this treatment is known to reduce the number of episodes as well as duration of diarrhea.	2015	25796406
characterization of lactobacillus isolated from dairy samples for probiotic properties.	in the present study twelve lactobacillus isolates (lbs 1-lbs 12) were characterized for probiotic properties. out of the twelve, eight isolates (lbs 1-6, 8 and 11) were bile resistant (survival > 50% at 0.3% bile salt w/v) and five isolates (lbs 1, 2, 5, 6 and 11) were found acid ph value resistant (survival > 50% at ph 3). all twelve isolates inhibited the growth of staphylococcus aureus whereas isolate lbs 2 also inhibited the growth of escherichia coli and salmonella typhimurium. antibiotic ...	2015	25771244
effects of transparent exopolymer particles and suspended particles on the survival of salmonella enterica serovar typhimurium in seawater.	the bacterium salmonella enterica can infect marine mammals and has been increasingly implicated in seafood-borne disease outbreaks in humans. despite the risk this zoonotic agent poses to animals and people, little is known regarding the environmental factors that affect its persistence in the sea. the goal of this study was to evaluate the impact of two constituents on the survival of salmonella in the marine environment: transparent exopolymer particles (tep) and suspended particles. a decay ...	2015	25764565
formate hydrogen lyase mediates stationary-phase deacidification and increases survival during sugar fermentation in acetoin-producing enterobacteria.	two fermentation types exist in the enterobacteriaceae family. mixed-acid fermenters produce substantial amounts of lactate, formate, acetate, and succinate, resulting in lethal medium acidification. on the other hand, 2,3-butanediol fermenters switch to the production of the neutral compounds acetoin and 2,3-butanediol and even deacidify the environment after an initial acidification phase, thereby avoiding cell death. we equipped three mixed-acid fermenters (salmonella typhimurium, s. enteriti ...	2015	25762991
in vitro antibacterial and chemical properties of essential oils including native plants from brazil against pathogenic and resistant bacteria.	the antimicrobials products from plants have increased in importance due to the therapeutic potential in the treatment of infectious diseases. therefore, we aimed to examine the chemical characterisation (gc-ms) of essential oils (eo) from seven plants and measure antibacterial activities against bacterial strains isolated from clinical human specimens (methicillin-resistant staphylococcus aureus (mrsa) and sensitive (mssa), escherichia coli, pseudomonas aeruginosa, salmonella typhimurium) and f ...	2015	25757433
low aspect ratio micropores for single-particle and single-cell analysis.	this paper describes microparticle and bacterial translocation studies using low aspect ratio solid-state micropores. micropores, 5 μm in diameter, were fabricated in 200 nm thick free-standing silicon nitride membranes, resulting in pores with an extremely low aspect ratio, nominally 0.04. for microparticle translocation experiments, sulfonated polystyrene microparticles and magnetic microbeads in size range of 1-4 μm were used. using the microparticle translocation characteristics, we find tha ...	2015	25752985
acrb drug-binding pocket substitution confers clinically relevant resistance and altered substrate specificity.	the incidence of multidrug-resistant bacterial infections is increasing globally and the need to understand the underlying mechanisms is paramount to discover new therapeutics. the efflux pumps of gram-negative bacteria have a broad substrate range and transport antibiotics out of the bacterium, conferring intrinsic multidrug resistance (mdr). the genomes of pre- and posttherapy mdr clinical isolates of salmonella typhimurium from a patient that failed antibacterial therapy and died were sequenc ...	2015	25737552
intraepithelial lymphocyte numbers and histomorphological parameters in the porcine gut after enterococcus faecium ncimb 10415 feeding in a salmonella typhimurium challenge.	salmonellae are among the most widespread sources of foodborne infections and salmonella typhimurium, in particular, is correlated with human disease caused by the consumption of contaminated pork. intraepithelial lymphocytes (iel) have early contact with intestinal antigens and play an important role in the detection of pathogenic bacteria. the objective of this study was to determine whether a presumed probiotic enterococcus faecium strain could improve histomorphological and immune system-rel ...	2015	25669594
oligomerization of esculin improves its antibacterial activity and modulates antibiotic resistance.	in this particular study, the antibacterial activity of esculin and oligomer fractions was assessed. mic values of esculin and its oligomer fractions as well as of some antibiotics against gram-positive and gram-negative strains and against escherichia coli multiresistant variants were determined by the standard broth microdilution method. both esculin and oligoesculin fractions exhibited antibacterial effect against reference strains; staphylococcus aureus, enterococcus faecalis, salmonella ent ...	2015	25248728
antimicrobial effect against different bacterial strains and bacterial adaptation to essential oils used as feed additives.	the aim of this study was to evaluate the antimicrobial activity and determine the minimum bactericidal concentration (mbc) of the essential oils derived from origanum vulgare (oregano), melaleuca alternifolia (tea tree), cinnamomum cassia (cassia), and thymus vulgaris (white thyme) against salmonella typhimurium, salmonella enteritidis, escherichia coli, staphylococcus aureus and enterococcus faecalis. the study also investigated the ability of these different bacterial strains to develop adapt ...	2015	26424908
plasma deposition of silver nanoparticles on ultrafiltration membranes: antibacterial and anti-biofouling properties.	a novel and versatile plasma reactor was used to modify polyethersulphone commercial membranes. the equipment was applied to: i) functionalize the membranes with low-temperature plasmas, ii) deposit a film of poly(methyl methacrylate) (pmma) by plasma enhanced chemical vapor deposition (pecvd) and, iii) deposit silver nanoparticles (snp) by gas flow sputtering. each modification process was performed in the same reactor consecutively, without exposure of the membranes to atmospheric air. scannin ...	2015	26166926
synthesis, structural characterization and biological activity of novel cyclohexane-1,3-dione ligands and their metal complexes.	some new zn(ii) and cu(ii) complexes [cu(l1)(oac)2]∙h2o, [cu(l1)(no3)h2o]∙no3∙3.5h2o, [zn(l1)(no3)2]∙4.5h2o, [zn(l1)(oac)2(h2o)2]∙3h2o, [cu2(l2)(oac)4]∙2h2o∙2dmf, [cu(l2)2]∙2no3∙1.5dmf∙h2o, [zn(l2)2(no3)2]∙dmf and [zn2(l2)(oac)4(h2o)4]∙5h2o; l1 = 2-[2-(2-methoxyphenyl)hydrazono]cyclohexane-1,3-dione and l2 = 2-[2-(3-nitrophenyl)hydrazono]cyclohexane-1,3-dione were synthesized and characterized by ir, 1h-nmr,13c-nmr and ultraviolet (uv-vis.) spectroscopy, elemental analysis, magnetic susceptibili ...	2015	26007185
validation of a novel rinse and filtration method for efficient processing of fresh produce samples for microbiological indicator enumeration.	several methods have been described to prepare fresh produce samples for microbiological analysis, each with its own advantages and disadvantages. the aim of this study was to compare the performance of a novel combined rinse and membrane filtration method to two alternative sample preparation methods for the quantification of indicator microorganisms from fresh produce. decontaminated cantaloupe melons and jalapeño peppers were surface inoculated with a cocktail containing 10(6) cfu/ml escheric ...	2015	25719876
improved properties and microbiological safety of novel cottage cheese containing spices.	the study focuses on developing novel cottage cheese containing spices with acceptable sensory properties, increased biological value and extended shelf life. thirty types of cheese with added fresh or dried parsley, dill, pepper, garlic and rosemary were produced. characterisation of phenolic compounds, antioxidant capacity and antibacterial activity of spices and cheese samples were evaluated. the cheese containing fresh pepper and fresh and dried herbs showed excellent sensory properties, wit ...	2015	27904380
cloning and sequencing of hfq (host factor required for synthesis of bacteriophage q beta rna) gene of salmonella typhimurium isolated from poultry.	the aim was to clone and sequence hfq gene of salmonella typhimurium strain pm-45 and compare its sequence with hfq gene of other serovar of salmonella.	2015	27047143
histone deacetylase inhibitors promote mitochondrial reactive oxygen species production and bacterial clearance by human macrophages.	broad-spectrum histone deacetylase inhibitors (hdaci) are used clinically as anticancer agents, and more isoform-selective hdaci have been sought to modulate other conditions, including chronic inflammatory diseases. mouse studies suggest that hdaci downregulate immune responses and may compromise host defense. however, their effects on human macrophage antimicrobial responses are largely unknown. here, we show that overnight pretreatment of human macrophages with hdaci prior to challenge with s ...	2015	26711769
impact of t300a variant of atg16l1 on antibacterial response, risk of culture positive infections, and clinical course of crohn's disease.	autophagy-related 16-like 1 (atg16l1) deficiency leads to impaired cellular autophagy and bacterial degradation as well as an altered cytokine production. the single-nucleotide polymorphism rs2241880 (t300a) is associated with an increased risk for crohn's disease (cd). atg16l1 polymorphisms could therefore have an impact on the risk of infectious complications and disease course in cd. we examined the impact of the t300a genotype on the antibacterial response toward a panel of pathogenic bacter ...	2015	26673830
[study of rapid species identification of bacteria in water].	multi-wavelength ultraviolet visible (uv-vis) transmission spectra of bacteria combined the forward scattering and absorption properties of microbes, contains substantial information on size, shape, and the other chemical, physiological character of bacterial cells, has the bacterial species specificity, which can be applied to rapid species identification of bacterial microbes. four different kinds of bacteria including escherichia coli, staphylococcus aureus, salmonella typhimurium and klebsie ...	2015	26669181
reporters for single-cell analysis of colicin ib expression in salmonella enterica serovar typhimurium.	colicins are toxins that mediate interference competition in microbial ecosystems. they serve as a "common good" for the entire producer population but are synthesized by only few members which pay the costs of colicin production. we have previously shown that production of colicin ib (cib), a group b colicin, confers a competitive advantage to salmonella enterica serovar typhimurium (s. tm) over commensal e. coli strains. here, we studied regulation of s. tm cib expression at the single cell le ...	2015	26659346