Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| purification and biological characterization of bacterially expressed recombinant buffalo prolactin. | recombinant prolactin (prl) from water buffalo (bubalus bubalis) has been cloned and expressed in a prokaryotic expression system. the hormone was also successfully refolded into a biologically active form. total rna was purified from buffalo pituitaries and the buprl cdna was synthesized using primers designed on bovine prl sequence. this prolactin cdna was cloned in a pet 28a vector and expressed in escherichia coli strain bl21(de3)plyss. most of the expressed protein was present as insoluble ... | 2010 | 21108131 |
| sequence of specific mitochondrial 16s rrna gene fragment from egyptian buffalo is used as a pattern for discrimination between river buffaloes, cattle, sheep and goats. | characterization of molecular markers and the development of better assays for precise and rapid detection of domestic species are always in demand. this is particularly due to recent food scares and the crisis of biodiversity resulting from the huge ongoing illegal traffic of endangered species. the aim of this study was to develop a new and easy method for domestic species identification (river buffalo, cattle, sheep and goat) based on the analysis of a specific mitochondrial nucleotide sequen ... | 2010 | 21116860 |
| buffalo hepcidin: characterization of cdna and study of antimicrobial property. | hepcidin, a novel gene encoded, 25 residue, 2-3 kda cysteine rich cationic peptide synthesized in liver cells play an important role in iron metabolism in addition to its antimicrobial activity. in this study hepcidin cdna expressed on hepatocytes of bubalus bubalis has been characterized and the antibacterial activity of buffalo hepcidin analog has been determined. the complete buffalo hepcidin sequence is of 324 bp and have an orf of 249 nucleotides from 14 to 262. the sequence analysis at nuc ... | 2010 | 21161377 |
| the effect of season on oocyte quality and developmental competence in italian mediterranean buffaloes (bubalus bubalis). | at italian latitudes, buffalo (bubalus bubalis) is a seasonally polyestrous species, showing an improved reproductive efficiency when daylight decreases (autumn). the aim of the present study was to evaluate the influence of the season on buffalo oocyte recovery rate, on oocyte quality, assessed on morphological basis, and developmental competence after in vitro fertilization. for this purpose, buffalo ovaries were collected from a local abattoir and the oocytes obtained by aspirating the follic ... | 2010 | 21168984 |
| [genetic characteristics on exon 4 of prolactin gene in 12 water buffalo populations]. | the prolactin exerts obvious adjustment and control function for mammary gland development, lactation and milk protein gene expression in water buffalo. in this study the sequence features and polymorphisms of the exon 4 in prolactin gene were examined in 385 individuals which came from 12 river and swamp type buffalo populations using dna direct sequencing and pcr-sscp methods. the results showed that the sequence of exon 4 in prolactin gene was consists of 180 nucleotides, the fragment had hig ... | 2010 | 21174345 |
| identification of capacitation associated tyrosine phosphoproteins in buffalo (bubalus bubalis) and cattle spermatozoa. | at ejaculation mammalian sperm lack fertilizing ability as they are released in a functionally immature form. the capacity to fertilize eggs is only acquired after they have been educated in the female reproductive tract and this phenomenon is termed as capacitation. sperm capacitation includes a cascade of biochemical modifications, including cholesterol efflux, ca(2+) influx and camp/pka-dependent/independent protein tyrosine phosphorylation which is specifically considered as the biochemical ... | 2010 | 21177049 |
| evidence of congenital transmission of neospora caninum in naturally infected water buffalo (bubalus bubalis) fetus from brazil. | the aim of this study was to determine the congenital infection by neospora caninum in the water buffalo (bubalus bubalis), a natural intermediate host. nine pregnant water buffalos, raised under free-grazing condition, were slaughtered, and their fetuses were collected. samples of brain and thoracic fluid were obtained from those fetuses, with gestational ages ranging from 2 to 5 months. the dna of n. caninum was detected and identified in the brain of one of those fetuses, using two pcr assays ... | 2010 | 21181191 |
| enhancing reproductive performance in domestic dairy water buffalo (bubalus bubalis). | the purpose of the review is to describe the factors that affect fertility in domestic water buffalo (bubalus bubalis) and the techniques that enable an improvement in reproductive performance. on italian and latin american farms where natural mating is practiced and bulls are always present in the herd, the inter-calving interval is approximately 400 days and the culling rate is lower than 15%. the buffalo has a tendency for seasonal reproductive activity. reproduction is favoured when there is ... | 2010 | 21755690 |
| genetic parameters for milk, fat and protein yields in murrah buffaloes (bubalus bubalis artiodactyla, bovidae). | the objective of the present study was to estimate genetic parameters for test-day milk, fat and protein yields and 305-day-yields in murrah buffaloes. 4,757 complete lactations of murrah buffaloes were analyzed. co-variance components were estimated by the restricted maximum likelihood method. the models included additive direct genetic and permanent environmental effects as random effects, and the fixed effects of contemporary group, milking number and age of the cow at calving as linear and q ... | 2010 | 21637608 |
| identification and validation of reference genes for gene expression studies in water buffalo. | in gene expression analysis, a key step to obtain informative data from reverse transcription quantitative pcr (rt qpcr) assay is normalization, that is usually achieved by ratio to correct the abundance of the gene of interest against that of an endogenous reference gene. the finding of such reference genes, ideally expressed in a stable way in multiple tissue samples and in different experimental conditions, is a non-trivial problem. in this work, a set of genes potentially useful as reference ... | 2010 | 22444257 |
| effect of bromocryptine on hormones and milk secretion in murrah buffaloes ( bubalus bubalis). | an experiment was carried out on 10 advance pregnant murrah buffaloes to determine the role of hormones in milk secretion around parturition. experimental animals were administered with a single injection of bromocryptine, @ 100 μg/kg bw, for 5 days before expected calving, whereas control group buffaloes were injected with placebo injections. blood samples collected before parturition (-5,-4,-3,-2,-1 days), on day of parturition (day-0) and on day 1, 2, 3, 4, 5, 10 and 15 post partum were analy ... | 2010 | 22444132 |
| effectiveness of poll stunning water buffalo with captive bolt guns. | practical experience has indicated that shooting water buffalo with a captive bolt gun in the front of the head does not always produce an effective stun. slaughtermen have been claiming that the poll position is more reliable, but under present eu regulations this shooting position is not allowed for domesticated bovines. this study examined the effectiveness of shooting water buffalo with captive bolt guns in the poll region. the depth of concussion was assessed in 30 water buffalo from physic ... | 2009 | 22063979 |
| characterization of the glutamate dehydrogenase activity of gigantocotyle explanatum and gastrothylax crumenifer (trematoda: digenea). | glutamate dehydrogenase (gldh) (ec 1.4.1.3) is a ubiquitous enzyme, which is present at the protein and carbohydrate metabolism crossroads. the enzyme activity was investigated in biliary and rumen amphistomes, gigantocotyle explanatum and gastrothylax crumenifer, respectively, infecting the indian water buffalo bubalus bubalis. the enzyme activity was consistently higher in g. explanatum as compared to g. crumenifer, where nad(h) was utilized as coenzyme and the ph optima was recorded at 8. the ... | 2009 | 23129890 |
| in vitro production of cattlexbuffalo hybrid embryos using cattle oocytes and african buffalo (syncerus caffer caffer) epididymal sperm. | interspecies hybridization of bovids occurs between domestic cattle and at least three other species; american bison (bison bison), yak (bos grunniens) and banteng (bos banteng). birth of a cattlexbuffalo (bubalus bubalis) hybrid has reportedly occurred in russia and in china, but these reports were not authenticated. such hybrids could be important in improving livestock production and management of diseases that impede production in tropical africa. this study investigated hybridization betwee ... | 2009 | 19118889 |
| effect of bupivacaine on epidural analgesia produced by xylazine or medetomidine in buffaloes (bubalus bubalis). | to evaluate and compare the effect of epidural bupivacaine on analgesia produced by epidural xylazine or medetomidine in buffaloes. | 2009 | 19121162 |
| characterization and expression profile of complete functional domain of granulysin/nk-lysin homologue (buffalo-lysin) gene of water buffalo (bubalus bubalis). | granulysin (gnly)/nk-lysin (nkl) is an effector antimicrobial cationic peptide expressed in the cytotoxic and natural killer lymphocytes. we report here cdna sequence (405bp) encoding the complete functional domain of buffalo-lysin (bu-lysin), and its expression profile in the various tissues. the nucleotide sequence of bu-lysin exhibited >85% identity with the bovine lysin. comparison of the deduced amino acid sequence of bu-lysin with those of gnly/nkl of different species revealed the conserv ... | 2009 | 19136155 |
| detection of bovine viral diarrhea virus from three water buffalo fetuses (bubalus bubalis) in southern italy. | bovine viral diarrhea virus (bvdv) is an important pathogen that primarily infects ruminants, leading to several clinical problems including abortion. bvdv-specific antibodies were reported in a wide range of hosts within domestic and wildlife animal populations, and serological studies also indicated bvdv infection in buffaloes. the purpose of this study was to analyze the presence of bvdv in 2 water buffalo (bubalus bubalis) herds with a history of abortion. virus isolation from aborted fetuse ... | 2009 | 19139516 |
| use of real-time pcr technique in studying rumen cellulolytic bacteria population as affected by level of roughage in swamp buffalo. | a real-time polymerase chain reaction approach was used in this study to determine the population of major ruminal bacterial species (fibrobacter succinogenes, ruminococcus albus, and ruminococcus flavefaciens) in digesta and rumen fluid of swamp buffalo (bubalus bubalis). four rumen-fistulated, male swamp buffalo were randomly assigned according to a 4 x 4 latin square design to evaluate the effect of the urea-treated rice straw (roughage source)-to-concentrate ratio on cellulolytic bacterial d ... | 2009 | 19018588 |
| populations of domesticated cattle and buffalo in the western forest complex of thailand and their possible impacts on the wildlife community. | the western forest complex (wefcom) of thailand is comprised of many protected areas and has one of the highest wildlife populations in the country. populations of wildlife in the wefcom have decreased dramatically over recent years. rapid economic development has resulted in the conversion of forest into agricultural and pastoral land, which has directly and indirectly impacted the wildlife community. this research aimed to evaluate populations of domesticated cattle (bos indicus) and buffalo ( ... | 2009 | 19036494 |
| prevalence of helminths in water buffaloes in hunan province, china. | the prevalence of helminths in the asian water buffaloes (bubalus bubalis) was investigated in hunan province, people's republic of china between april 2005 and october 2007. a total of 359 adult buffaloes slaughtered at local abattoirs in 12 representative geographical locations in hunan province were examined for the presence of helminths. the worms were examined, counted and identified to species according to existing keys and descriptions. a total of 13 helminth species were found representi ... | 2009 | 18704740 |
| lipid peroxidation, mitochondrial membrane potential and dna integrity of spermatozoa in relation to intracellular reactive oxygen species in liquid and frozen-thawed buffalo semen. | several factors affect sperm motility and functional integrity during preservation; the damage caused by reactive oxygen species (ros) being an important factor. the present study investigated intracellular ros generation and its relationship with sperm motility, lipid peroxidation (lpo), mitochondrial membrane potential (mmp) and dna integrity during preservation (liquid preservation at 4 degrees c and cryopreservation) of buffalo semen. fifty-six ejaculates, eight each from seven buffalo bulls ... | 2009 | 19010614 |
| prognostic value of various spermatological attributes as predictors of zona binding and zona penetration of buffalo (bubalus bubalis) semen. | twenty-four ejaculates from six (four ejaculates each) surti buffalo bulls aged 4-8 years were used to assess various attributes of spermatozoa influencing the zona-binding and zona-penetration tests. ejaculates from each bulls were subjected to in vitro sperm--zona binding and sperm--zona penetration tests (four replicates per bull) using immature buffalo oocytes. the average number of spermatozoa bound per oocyte was 27.79 +/- 5.90. the average number of spermatozoa penetrated per oocyte was 3 ... | 2009 | 18673329 |
| malignant catarrhal fever: a review. | malignant catarrhal fever (mcf) is a fatal lymphoproliferative disease of cattle and other ungulates caused by the ruminant gamma-herpesviruses alcelaphine herpesvirus 1 (alhv-1) and ovine herpesvirus 2 (ovhv-2). these viruses cause inapparent infection in their reservoir hosts (wildebeest for alhv-1 and sheep for ovhv-2), but fatal lymphoproliferative disease when they infect mcf-susceptible hosts, including cattle, deer, bison, water buffalo and pigs. mcf is an important disease wherever reser ... | 2009 | 18760944 |
| influence of igf-i on buffalo (bubalus bubalis) spermatozoa motility, membrane integrity, lipid peroxidation and fructose uptake in vitro. | the objective of the present experiment was to examine the influence of mean physiological concentration of insulin-like growth factor-i (igf-i) on frozen-thawed surti buffalo (bubalus bubalis) spermatozoa functional parameters, i.e., motility, plasmalemma integrity, acrosomal integrity, functional membrane integrity, lipid peroxidation and fructose uptake in vitro. frozen-thawed semen samples (n=6) were washed in tris buffer and divided into two equal parts (control and igf-i groups). only in t ... | 2009 | 18804927 |
| factors affecting the quality of cryopreserved buffalo (bubalus bubalis) bull spermatozoa. | storage of buffalo (bubalus bubalis) bull semen in the cryopreserved state is discussed in this article. fertility rate in buffalo following artificial insemination with frozen-thawed semen is reviewed. to better understand the freezability of bubaline spermatozoa, the available data on biochemical components and the activity of specific enzymes of semen/spermatozoa are given. moreover, the major factors that may influence the post-thaw viability and fertility of buffalo spermatozoa are examined ... | 2009 | 18954384 |
| capacitation-associated protein tyrosine phosphorylation starts early in buffalo (bubalus bubalis) spermatozoa as compared to cattle. | a comparative study was conducted on protein tyrosine phosphorylation events of capacitating sperm of two ruminant species, cattle and buffalo. ejaculated cattle and buffalo bull spermatozoa were suspended separately in sp-talp medium at 50 x 10(6)ml(-1) and incubated at 38.5 degrees c with 5% co(2) in air in the absence or presence of heparin for a period of 6h. the extent of sperm capacitation after various periods of incubations was assessed by lysophosphatidyl choline-induced acrosome reacti ... | 2009 | 18358647 |
| effect of breeding season on in vivo oocyte recovery and embryo production in non-descriptive indian river buffaloes (bubalus bubalis). | the present study was carried out to examine the effect of season on in vivo oocyte recovery and embryo production in non-descriptive, indian river buffaloes (bubalus bubalis). ovum pick up (opu) was conducted twice a week for 8 weeks during peak (october-march) and low (april-september) breeding season in live buffaloes (n=6). opu was performed using ultrasound equipment with a 5mhz transvaginal transducer, a single lumen 18-gauge, 55-cm long needle and a constant vacuum pressure of 110mmhg. th ... | 2009 | 18440168 |
| molecular cloning and characterization of lingual antimicrobial peptide cdna of bubalus bubalis. | antimicrobial peptides form a crucial component of innate immune system, making it a highly effective first line of defense in animals. in the study, lingual antimicrobial peptide cdna of bubalus bubalis has been characterized. the characterized cdna has complete orf of 195 bases. the signal sequence of buffalo lap comprised of n-terminal 1-20 amino acids and mature peptide from 23-64 amino acids. the percentage of similarity of buffalo lap and buffalo ebd at nucleotide and amino acid level was ... | 2009 | 18558415 |
| characterization of tlr4 signaling in water buffalo (bubalus bubalis). | lipopolysaccharide (lps) treatment of peripheral blood mononuclear cells (pbmc) isolated from the water buffalo resulted in the activation of tlr signaling intermediates as supported by the western blot of perk. activation of erk resulted in phosphorylation of ikappab-alpha which lead to its degradation which in turn followed by nuclear translocation of nf-kappab, which is also supported by the western blot analysis. the nuclear translocation of nf-kappab culminated in the induction of mrna expr ... | 2009 | 18648999 |
| structure-function studies of bubalus bubalis lingual antimicrobial peptide analogs. | antimicrobial peptides expressed on different epithelial lining are major components of the innate immune system. based on the deduced amino acid sequence of bubalus bubalis lingual antimicrobial peptide (lap) cdna (accession no. dq458768), five overlapping peptides lap(23-55), lap(42-64), lap(21-64), lap(1-26) and lap(1-64) were synthesized using solid phase fluorenylmethoxycarbonyl (fmoc) chemistry. circular dichroism spectroscopy of synthesized peptides revealed predominantly beta-structure f ... | 2009 | 18651233 |
| eimeria spp. in brazilian water buffalo. | eimeria species are frequently found in water buffalo (bubalus bubalis) in brazil. here, we report those eimeria spp. that infect buffalos during their first year of life. fresh fecal samples were examined from 2 groups (1 group/yr for 2 yr, 2000-2002), each with 18 water buffalo calves (both sexes), from birth through 12 mo of age, in selvíria, ms, brazil. five oocyst morphotypes were observed, i.e., eimeria ellipsoidalis and eimeria zuernii, both previously described from water buffalo, and 3 ... | 2009 | 18662041 |
| detection of nontuberculous mycobacteria from water buffalo raw milk in brazil. | milk is an important nutritional source to man and water buffalo raw milk is used to produce mozzarella cheese. products from unpasteurized milk have been associated with certain infectious diseases and can carry pathogenic mycobacteria. nontuberculous mycobacteria (ntm) are emerging pathogens causing opportunistic infections in humans and animals. the objectives of this study were to demonstrate the presence of mycobacteria in water buffaloes' milk and to determine their role as possible source ... | 2009 | 19527843 |
| classification of new bvdv isolates from philippine water buffalo using the viral e2 region. | characterization of bovine viral diarrhea virus (bvdv) isolates has been focused of several studies this last decade. until now lots of new strains are being unfolded maybe due to the viral fast mutation ability. as we focused our research on water buffalo immunology, we were able to identify a probable new bvdv isolates. rna was extracted from water buffalo blood in the philippines. the extracted rna was reverse-transcribed and synthesized cdna. oligonucleotide primers from the viral e2 region ... | 2009 | 19455512 |
| dominance of methanomicrobium phylotype in methanogen population present in murrah buffaloes (bubalus bubalis). | to study the diversity of rumen methanogens in murrah buffaloes (bubalus bubalis) from north india by using 16s rrna gene libraries obtained from the pooled rumen content from four animals and using suitable software analysis. | 2009 | 19486281 |
| light and scanning electron microscopic studies of oviductal epithelium in thai swamp buffalo (bubalus bubalis) at the follicular and luteal phases. | the purpose of this study was to investigate the morphological changes in the epithelium of thai swamp buffalo oviducts at the follicular and luteal phases by histological technique and scanning electron microscopy. the samples from the infundibulum, ampulla, isthmus and uterotubal junction (utj) of the oviduct were taken immediately after slaughter at the local abattoir. noticeable cyclic changes were observed on the epithelial surface of the infundibulum and ampulla, but few changes were prese ... | 2009 | 19416492 |
| a point mutation in the splice donor site of intron 7 in the alphas2-casein encoding gene of the mediterranean river buffalo results in an allele-specific exon skipping. | 2009 | 19422363 | |
| helmintic infections in water buffaloes on italian farms: a spatial analysis. | the present paper reports the results of a cross-sectional survey aimed at obtaining up-to-date information on the spatial distribution of different groups and/or species of helminths in water buffaloes from central italy. geographical information systems (gis) and spatial analysis were used to plan the sampling procedures, to display the results as maps and to detect spatial clusters of helminths in the study area. the survey was conducted on 127 water buffalo farms, which were selected in the ... | 2009 | 19440965 |
| tolerance to water buffalo milk in a child with cow milk allergy. | 2009 | 19441608 | |
| high-resolution haplotype block structure in the cattle genome. | the bovine hapmap consortium has generated assay panels to genotype ~30,000 single nucleotide polymorphisms (snps) from 501 animals sampled from 19 worldwide taurine and indicine breeds, plus two outgroup species (anoa and water buffalo). within the larger set of snps we targeted 101 high density regions spanning up to 7.6 mb with an average density of approximately one snp per 4 kb, and characterized the linkage disequilibrium (ld) and haplotype block structure within individual breeds and grou ... | 2009 | 19393054 |
| fatal onion (allium cepa) toxicosis in water buffalo (bubalus bubalis). | toxicosis caused by the ingestion of onion (allium cepa) by 5 water buffalo (bubalus bubalis) occurred in the district of caçador, santa catarina, brazil. the water buffalo died after ingestion of a large quantity of onion that had been left in the pasture. clinical signs started 8 days postingestion and were characterized by pale mucous membranes, lethargy, and dark urine. at necropsy, pieces of onions were found in the rumen of 1 animal. the carcass smelled strongly of onion, and the kidneys a ... | 2009 | 19407101 |
| feed supplementation prevents post-conception decline in milk progesterone concentrations associated with production stress in dairy buffaloes (bubalus bubalis). | the onset of pregnancy may be associated with hormonal changes and decline in milk yield of buffaloes. to investigate this, forty lactating buffaloes from 1st to 23rd weeks post-conception were selected. the animals were assigned to three treatments: prt, prs, nprt and three milk yielding groups hmy, 66 to 75 l/week, n = 12; mmy, 56 to 65 l/week, n = 16; lmy, 46 to 55 l/week, n = 12). milk samples were collected on alternate weeks and analyzed with ultrasonic milk analyzer. eia was used for milk ... | 2009 | 19107569 |
| fluoride-induced oxidative stress in rat's brain and its amelioration by buffalo (bubalus bubalis) pineal proteins and melatonin. | fluoride (f) becomes toxic at higher doses and induces some adverse effects on various organs, including brain. the mechanisms underlying the neurotoxicity caused by excess fluoride still remain unknown. the aims of this study were to examine f-induced oxidative stress (os) and role of melatonin (mel) and buffalo pineal proteins (pp) against possible f-induced os in brain of rats. the 24 rats were taken in present study and were divided into four groups: control, f, f + pp, and f + mel. the f gr ... | 2009 | 19159082 |
| pregnancies established from handmade cloned blastocysts reconstructed using skin fibroblasts in buffalo (bubalus bubalis). | handmade cloning (hmc), a simple, micromanipulation-free cloning technique, has been applied for the production of cloned embryos and offspring in many livestock species. the objective of the present study was to compare the effect of donor cell type on developmental competence of hmc embryos and to explore the possibility of establishing pregnancies using these embryos in buffalo. after technical optimization of the hmc procedure for in vitro development of cloned blastocysts, various donor cel ... | 2009 | 19168209 |
| the effect of addition of equine chorionic gonadotropin to a progesterone-based estrous synchronization protocol in buffaloes (bubalus bubalis) under tropical conditions. | poor estrus expression and anestrus decrease the reproductive efficiency of buffaloes. the objective of this study was to determine whether the addition of equine chorionic gonadotropin (ecg) to an estrous synchronization protocol and timed insemination could improve ovulation and pregnancy rates of anestrous buffalo cows under tropical conditions. the study population comprised 65 lactating murrah buffalo cows which were assigned to cidr (n=33) or cidr+ecg (n=32) treatment groups. cows in the c ... | 2009 | 19181376 |
| preliminary crystallographic study of hemoglobin from buffalo (bubalus bubalis): a low oxygen affinity species. | hemoglobin is a tetrameric, iron-containing metalloprotein, which plays a vital role in the transportation of oxygen from lungs to tissues and carbon dioxide back to lungs. though good amount of work has already been done on hemoglobins, the scarcity of data on three dimensional structures pertaining to low oxygen affinity hemoglobins from mammalian species, motivated our group to work on this problem specifically. herein, we report the preliminary crystallographic analysis of buffalo hemoglobin ... | 2009 | 19200047 |
| detection of coxiella burnetii in buffaloes aborted fetuses by is111 dna amplification: a preliminary report. | the aim of this study was to evaluate by pcr analyses the presence of coxiella burnetii infection in fetuses of water buffalo (bubalus bubalis) in the campania region (southern italy). samples were collected only from aborted fetuses and c. burnetii presence was evaluated by one-tube nested pcr amplification of the is111 repetitive element. of the 164 fetuses examined 14 (17.5%) were positive after dna amplification, showing that c. burnetii occurs in this population of water buffaloes. however, ... | 2009 | 19232419 |
| butylated hydroxytoluene inclusion in semen extender improves the post-thawed semen quality of nili-ravi buffalo (bubalus bubalis). | the study was carried out to evaluate the potential impact of butylated hydroxytoluene (bht) on the frozen-thawed semen quality of nili-ravi buffalo bulls. ejaculated bull semen was extended in a tris-citrate egg yolk extender containing various concentrations of bht (0.5, 1.0, 2.0 and 3.0mm). semen was frozen at -196 degrees c using 50 x 10(6) spermatozoa per 0.5 ml straws. five straws from each treatment were thawed to assess the semen quality in terms of sperm motility, viability, plasma memb ... | 2009 | 19246080 |
| improvement of liquid and frozen-thawed semen quality of nili-ravi buffalo bulls (bubalus bubalis) through supplementation of fat. | the aim of the study was to investigate the effects of dietary fat on quality of liquid and frozen-thawed semen of nili-ravi buffalo bulls. adult bulls (n=21) were fed a balanced ration (con; n=7) or the same ration either containing sunflower oil (sf-o; n=7) or whole sunflower seeds (sf-s; n=7) for 63 days. body weight and body condition score of each bull was recorded on days 0, 30 and 60 of the experiment. semen was collected on days 39, 46, 53 and 60, frozen by a fast method and stored at -1 ... | 2009 | 19246083 |
| expression of orexin a and its receptor 1 in the choroid plexuses from buffalo brain. | the hypothalamic peptide orexin a, deriving from the proteolytic cleavage of the precursor molecule prepro-orexin, has a wide range of physiological effects including the regulation of feeding behaviour, neuroendocrine functions, sleep-wake cycle, and energy homeostasis. lowered excretion of orexin a into the cerebrospinal fluid (csf) plays a pathological role in animal and human narcolepsy. altered levels of orexin a into the csf have been also found in numerous disorders of the central nervous ... | 2009 | 19250669 |
| identification and quantification of alphas1, alphas2, beta, and kappa-caseins in water buffalo milk by reverse phase-high performance liquid chromatography and mass spectrometry. | a method for the simultaneous quantitation of alpha(s1), alpha(s2), beta, and kappa-caseins in water buffalo (bubalus bubalis) milk using reverse phase high-performance liquid chromatography was developed. the molecular masses of the peaks separated by the described chromatographic protocol were determined by esi-ms. alpha(s1)- and kappa-caseins were found to be heteromorphic in several individual milk samples. in particular, alpha(s1)-casein showed two peaks with a molecular mass of 23,490 da a ... | 2009 | 19256489 |
| isolation of two cdnas encoding mhc-dqa1 and -dqa2 from the water buffalo, bubalus bubalis. | in the present study, we explored structural and functional variations and possible duplication of the major histocompatibility complex (mhc)-dqa gene in water buffalo (bubalus bubalis). two cdna sequences, amplified from one individual water buffalo, were designated as bubu-dqa1 (dqa*0101) and -dqa2 (dqa*2001). the percentage of nucleotide and amino acid similarity between bubu-dqa1 and -dqa2 revealed that these sequences display more similarity to alleles of respective dqa1 and dqa2 genes from ... | 2009 | 19272657 |
| bovine papillomavirus type 1 dna and e5 oncoprotein expression in water buffalo fibropapillomas. | papillomas and fibropapillomas may occur in the skin and in different organs in animals. ten different genotypes of bovine papillomavirus (bpv) have been identified. bpv-1 through bpv-10 are all strictly species-specific, but bpv-1/2 may also infect other species such as equids, inducing fibroblastic tumors. bpv-1 and bpv-2 are associated with fibropapillomas in cattle; these tumors are formed by excessive proliferation of virus-infected dermal fibroblasts and epidermal keratinocytes. nine water ... | 2009 | 19276046 |
| comparative moleculo-immunological analysis of swamp- and riverine-type water buffaloes responses. | this moleculo-epidemiological and immunological study through cytokine response assessment was done to know the dynamics of cytokines in the initiation, persistence and association to physiological changes of a particular pathogen in water buffaloes. this is important to understand the magnitude and behavior of disease progression. water buffalo blood samples gathered from different places in the philippines revealed a 9.4%, 27.6%, 10.3% and 4.4% prevalence of bovine viral diarrhea virus (bvdv), ... | 2009 | 19285880 |
| pharmacokinetics, urinary excretion and plasma protein binding of danofloxacin following intravenous administration in buffalo calves (bubalus bubalis). | pharmacokinetics, urinary excretion and plasma protein binding of danofloxacin was investigated in buffalo calves following intravenous administration at the dose rate of 1.25 mg/kg to select the optimal dosage regimen of danofloxacin. drug concentrations in plasma and urine were measured by microbiological assaying. in vitro plasma protein binding was determined employing the equilibrium dialysis technique. the distribution and elimination of danofloxacin were rapid, as indicated by values (mea ... | 2009 | 19296232 |
| heterogeneous shedding of brucella abortus in milk and its effect on the control of animal brucellosis. | to ascertain whether in brucella abortus-infected water buffalo herds, the number of newly infected animals could be reduced by culling superspreaders (the animals secreting > or =10(4) cfu per ml of milk). | 2009 | 19298512 |
| quantification of water buffalo (bubalus bubalis) cytokine expression in response to inactivated foot-and-mouth disease (fmd) vaccine. | this study describes the quantification of cytokine expression of vaccinated water buffaloes with fmd inactivated vaccine. using real-time pcr quantification assay, expression of th1 (il-2, il-12p40, ifngamma); th2 (il-4, il-10) and inflammatory (il-6, tnfalpha) cytokines were quantified weekly for the entire three-week duration of the experiment. it was noted that ifngamma, il-10 and tnfalpha had peaked on week three post-vaccination while the remaining cytokines peaked on the second week and d ... | 2009 | 19303121 |
| occurrence of beta-casein fragments in cold-stored and curdled river buffalo (bubalus bubalis l.) milk. | the safeguard of river buffalo mozzarella cheese, a protected designation of origin dairy product, has prompted an analytical study to trace the milk and curd used as raw material in cheesemaking. this is to prevent the illegal use of milk or curd from different geographical areas outside of those indicated in the official production protocol. for this purpose, we studied primary proteolysis occurring in fresh and frozen milk and curd to identify a molecular marker that could indicate the raw ma ... | 2009 | 19307613 |
| non-invasive methods for identifying oocysts of sarcocystis spp. from definitive hosts. | because the excreted sporocysts and/or oocysts of various species of sarcocystis may not be discriminated morphologically, we sought to validate a diagnostic technique based on variation in the 18s rdna sequence. oocysts and/or sporocysts from three taxa of sarcocystis were collected from human, feline, and canine definitive hosts that had fed upon meats infected with the muscle cysts of sarcocystis hominis, sarcocystis fusiformis and a species of sarcocystis from water buffalo that could not be ... | 2009 | 19336258 |
| comparative therapeutic effect of moxidectin, doramectin and ivermectin on psoroptes mites infestation in buffalo (bubalus bubalis). | the aim of the present study was to carry out comparative therapeutic effect of moxidectin pour on, doramectin and ivermectin on psoroptes infestation in buffalo. a total of 318 buffalo in 77 small scale herds suspected to have mange mites were examined clinically and parasitologically. fifty-three (16.66%) buffalo in 25 herds were recorded to be infested; 51 (16.35%) with psoroptic mites, and two (0.31%) with chorioptic mites. buffalo with psoroptic mites were randomly allocated into three grou ... | 2009 | 19347598 |
| shedding of brucella abortus rough mutant strain rb51 in milk of water buffalo (bubalus bubalis). | the objective of this study was to determine if brucella abortus rough mutant strain rb51 (srb51) is eliminated in buffalo milk. thirty brucella-free female buffaloes were used in this study: ten 4-5 years old were inoculated with the triple of the recommended calfhood dose of srb51 by subcutaneous route, ten 2-3 years old at the first lactation were previously vaccinated twice as calves with triple the recommended calf dose of rb51, while five 4-5 years old and five 2-3 years old not vaccinated ... | 2009 | 19362381 |
| lingual antimicrobial peptide expresses in buffalo mammary gland. | mammalian beta-defensins are innate host defense peptides with potential as a therapeutic agent. present study reports expression of beta-defensin mrna transcript in teat mucosal layer of bubalus bubalis, which is homologous to lingual antimicrobial peptide (lap) of cattle. the pcr-amplified and cloned full-length cdna encodes for a putative peptide of 64 residues ( approximately 7 kda) with pi of 11.324 and possess characteristic features of beta-defensin family including presence of six evolut ... | 2009 | 19370457 |
| fish-mapping comparison between river buffalo chromosome 7 and sheep chromosome 6: assignment of new loci and comparison with hsa4. | synchronized peripheral blood lymphocytes from both river buffalo (bbu) and sheep (oar) were treated for late incorporation of both brdu and h-33258 to obtain r-banded preparations to be used for fish-mapping. ovine bac-clones were hybridized for three days on slides pre-exposed to uv light after h-33258 staining. the following loci were mapped: gpr103 (bbu7q13, oar6q13), tram1l1(oar6q13dist), ppp3ca (bbu7q21, oar6q15), snca (oar6q17), ppargc1a(bbu7q23, oar6q17), ugdh (bbu7q25prox, oar6q22prox), ... | 2009 | 19372676 |
| different promoter usage for cyp19 gene expression in buffalo ovary and placenta. | aromatase is the key enzyme for estrogen biosynthesis and is coded by cyp19 gene. the expression of cyp19 gene is regulated in tissue-specific manner by alternate use of different promoters. in this study, we have analyzed tissue-specific expression and their regulation of cyp19 gene in preovulatory and postovulatory stages of buffalo (bubalus bubalis) ovary and placenta. rt-pcr analysis showed that the cyp19 gene expression was significantly (p<0.05) higher in granulosa cells of large follicles ... | 2009 | 19375426 |
| reproductive biotechniques in buffaloes (bubalus bubalis): status, prospects and challenges. | the swamp buffalo holds tremendous potential in the livestock sector in asian and mediterranean countries. current needs are the faster multiplication of superior genotypes and the conservation of endangered buffalo breeds. recent advances in assisted reproductive technologies, including in vitro embryo production methodologies, offer enormous opportunities to not only improve productivity, but also to use buffaloes to produce novel products for applications to human health and nutrition. the us ... | 2009 | 19383257 |
| diagnosis of sarcocystis spp. in cattle (bos taurus) and water buffalo (bubalus bubalis) in northern vietnam. | our aim was to develop a method for species diagnosis and to obtain data on the prevalence of sarcocystis infections in cattle and water buffalo in the son la province of northern vietnam. meat samples of naturally infected animals were examined by light and electron microscopy as well as by molecular methods. a pcr of part of the 18s rdna gene followed by rflp analysis was modified to detect infections with different sarcocystis spp. in cattle and water buffaloes slaughtered in the son la provi ... | 2009 | 19783101 |
| loop-mediated isothermal amplification (lamp) detection of babesia orientalis in water buffalo (bubalus babalis, linnaeus, 1758) in china. | loop-mediated isothermal amplification (lamp) is a rapid method with high specificity and efficiency under isothermal condition using a set of four specifically designed primers that recognize six distinct sequences on the target gene. in this study, a lamp method was developed for specific detection of babesia orientalis in water buffalo (bubalus babalis, linnaeus, 1758). four primers were designed from the v4 hypervariable region of the 18s rrna gene of b. orientalis. blood samples were collec ... | 2009 | 19665847 |
| sequence analysis of morbillivirus cd150 receptor-signaling lymphocyte activation molecule (slam) of different animal species. | signaling lymphocyte activation molecule-slam (cd150) molecule has been reported as a putative receptor for most morbilliviruses for their respective host species. in this study, we determined the complete nucleotide sequence of the gene coding for the morbillivirus receptor-slam from the four species, namely, goat (capra hircus), sheep (ovis aries), indian cattle (bos indicus), and buffalo (bubalus bubalis). the nucleotide (nt) open reading frame sequence of slam gene in all the four species st ... | 2009 | 19669672 |
| factors affecting laboratory production of buffalo embryos: a meta-analysis. | in vitro fertilization (ivf) provides an excellent and inexpensive source of embryos for carrying out basic research on developmental physiology, farm animal breeding, and for commercial applications. meta-analysis of the results from different publications rather than a narrative review may provide a current status of this technology in buffalo (bubalus bubalis). in order to gain an idea of the factors affecting the ivf in buffalo, a review of the various studies conducted on buffalo ivf and a ... | 2009 | 19695691 |
| verocytotoxin-producing escherichia coli o26 in raw water buffalo (bubalus bubalis) milk products in italy. | escherichia coli 026 is known as a verocytotoxin-producing e. coli (vtec) organism that causes severe foodborne diseases such as hemorrhagic colitis and hemolytic uremic syndrome. although cattle are the most important reservoir of vtec, only a few reports on the role of water buffalo (bubalus bubalis) as a reservoir of vtec and on the presence of these organisms in their milk are available. however, in southern italy, where water buffalo are intensively reared, an outbreak of hemolytic uremic s ... | 2009 | 19722404 |
| validation and application of an immunofluorimetric assay for detection of serum free triiodothyronine and free thyroxine concentrations in buffalo (bubalus bubalis) under various physiological conditions. | the current study was designed to validate an immunofluorimetric method to measure free triiodothyronine (ft3) and free thyroxine (ft4) concentrations in the serum of buffalo and to develop a better understanding of thyroid metabolism in buffalo (bubalus bubalis) of different ages and reproductive statuses. free triiodothyronine and ft4 were determined in sera from 4 groups of 10 animals, each representing 5-10-year-old lactating buffalo, 2-3-year-old first calf heifers, 1-1.5-year-old virgin he ... | 2009 | 19737762 |
| identification of novel allelic variants of integrin beta 2 (itgb2) gene and screening for bubaline leukocyte adhesion deficiency syndrome in indian water buffaloes (bubalus bubalis). | a fragment of 570 bp corresponding to exon 5 and 6 of integrin beta 2 (itgb2) gene was amplified for screening d128g mutation in one hundred and fifty two buffaloes (bubalus bubalis) which causes bovine leukocyte adhesion deficiency syndrome (blad) in cattle, as well as to ascertain polymorphism. taqi pcr-rflp revealed no such mutation thus indicating the absence of bubaline leukocyte adhesion deficiency (bulad) allele in animals under study. however, the polymorphism studies using mspi restrict ... | 2009 | 19544212 |
| molecular cloning and characterization of cdna encoding buffalo (bubalus bubalis) cytochrome p450 aromatase in the ovary. | one of the predominant causes of poor reproduction in buffaloes is low levels of ovarian estrogens. a rate limiting enzyme in estrogen biosynthesis is cytochrome p450 aromatase (p450 arom), the product of cyp19 gene. in the present study cyp19 cdna was cloned and its 5'utr was characterized by 5'race in granulosa cells of large follicles. cyp19 transcripts with four different 5'utrs (206, 114, 90 and 3 bases) were found in buffalo granulosa cells of large ovarian follicles. interestingly, a pred ... | 2009 | 19544214 |
| clinical and biochemical studies on theileria annulata in egyptian buffaloes (bubalus bubalis) with particular orientation to oxidative stress and ketosis relationship. | this study was carried out on 68 theileria annulata naturally infected buffaloes in addition to 25 parasitologically free buffaloes distributed in small herds at dakahlia and gharbya governorates, egypt, to demonstrate the clinical picture associated with theileriosis in this buffaloes with particular emphasis to the oxidative stress and ketosis relationship. clinical signs recorded in infected buffaloes were in the form of fever, enlargement of one or more lymph node, ocular discharge, corneal ... | 2009 | 19559534 |
| effects of the seminal plasma zinc content and catalase activity on the semen quality of water buffalo (bubalus bubalis) bulls. | in order to determine zinc and catalase content of seminal plasma in the buffalo and to study their associations with the semen characteristics, 54 semen samples were collected from 10 buffalo bulls; semen volume and sperm concentration, gross and progressive motility and viability were evaluated, seminal plasma was then harvested by centrifugation and its zinc content was estimated by atomic absorption spectrophotometer and its catalase activity determined by using a commercial kit. the zinc co ... | 2009 | 19579933 |
| expressional dynamics of minisatellite 33.15 tagged spermatozoal transcriptome in bubalus bubalis. | transcriptionally quiescent spermatozoa have been established to be a repository of mrna coding for several functionally essential cellular proteins. this entourage of mrna is envisaged to be involved in post-fertilization and early embryogenesis. minisatellites tagged with mrna transcripts have been implicated with gene organization, regulation and function. however, the organization and expression of the minisatellite tagged transcript diversity, particularly in spermatozoa, remains unclear. | 2009 | 19583837 |
| optimization of embryo culture conditions for increasing efficiency of cloning in buffalo (bubalus bubalis) and generation of transgenic embryos via cloning. | cloning in bovine species is marred by low efficiency of blastocyst formation. any increase in the efficiency of blastocyst formation upon nuclear transfer will greatly enhance the efficiency of cloning. in the present study, the effect of various media, protein sources, and growth factors on the development of cloned buffalo embryos was evaluated. among various combinations tested, culture of cloned embryos in tcm-199 media on the feeder layer of buffalo oviductal epithelial cells (boec) in the ... | 2009 | 19594388 |
| improvement of in vitro oocyte maturation with lectin supplementation and expression analysis of cx43, gdf-9, fgf-4 and fibronectin mrna transcripts in buffalo (bubalus bubalis). | to optimize in vitro maturation (ivm) of quality oocytes for embryo production through ivf and scnt. | 2009 | 19629675 |
| immune response and viral persistence in indian buffaloes (bubalus bubalis) infected with foot-and-mouth disease virus serotype asia 1. | despite their potential role in the spread of foot-and-mouth disease (fmd), the immune response and viral persistence in fmd virus (fmdv)-infected indian buffaloes (bubalus bubalis) have been unexplored. we found similar kinetics of neutralizing antibody responses in the sera and secretory fluids of buffaloes following experimental fmdv asia 1 infection, but the lymphocyte-proliferative response in infected buffaloes was of low magnitude. despite inducing a significant systemic and secretory imm ... | 2009 | 19828770 |
| fast isoelectric focusing and antipeptide antibodies for detecting bovine casein in adulterated water buffalo milk and derived mozzarella cheese. | plasmin hydrolysis of water buffalo casein (cn) can liberate a peptide comigrating with bovine gamma(2)-cn. occurrence of this peptide may lead to false-positive detection of cow's milk for a genuine water buffalo cheese when it is analyzed by applying a fast version of the european official method for detecting bovine casein in water buffalo cheese. after isoelectric focusing of cn plasminolysates, performed according to the official method, immunoblot analysis with antipeptide antibodies was a ... | 2009 | 19831418 |
| detection of prion gene promoter and intron1 indel polymorphisms in anatolian water buffalo (bubalus bubalis). | bovine spongiform encephalopathy (bse) is a fatal disease caused by miss folded prion protein. studies in the cattle, comparing genetic data from bse diseased and healthy animals have shown that indel polymorphisms in the promoter and intron 1 of prnp gene were associated with disease susceptibility. several studies were conducted to find out allele and genotypic frequencies of indel polymorphisms in promoter and intron 1 of the cattle prnp gene. unlike domestic cattle and bison, no indel polymo ... | 2009 | 19912420 |
| short communication: detection of human torque teno virus in the milk of water buffaloes (bubalus bubalis). | forty-four raw milk and 15 serum samples from 44 healthy water buffaloes reared in caserta, southern italy, the most important region in europe for buffalo breeding, were examined to evaluate the presence of torque teno viruses (ttv) using molecular tools. furthermore, 8 pooled pasteurized milk samples (from dairy factories having excellent sanitary conditions) and 6 mozzarella cheese samples were also tested. four of the cheese samples were commercial mozzarella cheese; the remaining 2 were pre ... | 2009 | 19923596 |
| specific intracellular localization and antiviral property of genetic and splicing variants in bovine mx1. | in bovine mx1, only an amino acid substitution between ile and met at position 120 was detected by the nucleotide sequence and mismatched pcr-rflp technique. the ile variant was assumed to distribute mainly in the bovine population since the gene frequency was 79.3%. furthermore, we cloned water buffalo mx1 cdna, which showed 51 nucleotide and 20 amino acid substitutions in comparison with that of the cow. another kind of mx1 cdna, bovine mx1b cdna, was found and it was deduced to cause 27 amino ... | 2009 | 19951175 |
| microsatellite markers of water buffalo, bubalus bubalis--development, characterisation and linkage disequilibrium studies. | microsatellite markers are highly polymorphic and widely used in genome mapping and population genetic studies in livestock species. river buffalo, bubalus bubalis is an economically important livestock species, though only a limited number of microsatellite markers have been reported thus far in this species. | 2009 | 19843347 |
| pineal proteins upregulate specific antioxidant defense systems in the brain. | the neuroendocrine functions of the pineal affect a wide variety of glandular and nervous system processes. beside melatonin (mel), the pineal gland secretes and expresses certain proteins essential for various physiological functions. it has been suggested that the pineal gland may also have an antioxidant role due to secretory product other than mel. therefore, the present study was designed to study the effect of buffalo (bubalus bubalis) pineal proteins (pp) on the antioxidant defense system ... | 2009 | 20357930 |
| comparative rh maps of the river buffalo and bovine y chromosomes. | radiation hybrid maps were constructed for river buffalo and cattle y chromosomes. a total of 41 cattle-derived y-chromosome molecular markers were selected and tested with 2 previously described 5,000-rad whole-genome radiation hybrid (rh) panels (river buffalo - bburh(5000) and cattle - btarh(5000)) for generation of maps. among the initial 41 selected markers, a subset of 26 markers generated pcr products suitable for scoring with the bburh(5000) panel. of these, 19 markers (73%) were distrib ... | 2009 | 20016162 |
| comparative mapping and genomic annotation of the bovine oncosuppressor gene wwox. | wwox (ww domain-containing oxidoreductase) is the gene mapping at fra16d hsa16q23.1, the second most active common fragile site in the human genome. in this study we characterized at a detailed molecular level wwox in the bovine genome. first, we sequenced cdna from various tissues and obtained evidence in support of a 9-exon structure for the gene, similar to the human gene. then, we recovered bacs using exon tags and annotated the gene to a >1-mb genomic region of bta18 using the btau 4.0 geno ... | 2009 | 20016169 |
| [first notification of eimeria bareillyi (apicomplexa: eimeridae) in feces of buffalo calves (bubalus bubalis) naturally infected in minas gerais, brazil]. | in this study the evolution of eimeriosis in naturally infected calves was analyzed during 23 days starting at birth. the specie e. bareillyi was first identified in feces culture. later other species of eimeria already described in water buffalo were identified. the animals which died were submitted to necropsy, revealing macroscopic lesions in the ileum region. histological analyses of the ileum region showed acute necrotic enteritis and the presence of eimeria sp. in different developmental s ... | 2008 | 20059855 |
| a serological survey of crimean-congo haemorrhagic fever in animals in the sharkia governorate of egypt. | a surveillance survey was conducted over a period of 12 months between september 2004 and august 2005 by the tropical medicine department of zagazig university in collaboration with naval medical research unit no. 3 (namru-3), egypt, to evaluate the role of ruminants as a reservoir host for crimean-congo haemorrhagic fever virus (cchfv). a total of 1,022 serum samples from 313 cattle, 264 water buffalo (bubalus bubalis), 270 sheep and 175 goats were included in the survey. all samples were colle ... | 2008 | 20405447 |
| a first generation whole genome rh map of the river buffalo with comparison to domestic cattle. | the recently constructed river buffalo whole-genome radiation hybrid panel (bburh5000) has already been used to generate preliminary radiation hybrid (rh) maps for several chromosomes, and buffalo-bovine comparative chromosome maps have been constructed. here, we present the first-generation whole genome rh map (wg-rh) of the river buffalo generated from cattle-derived markers. the rh maps aligned to bovine genome sequence assembly btau_4.0, providing valuable comparative mapping information for ... | 2008 | 19108729 |
| transmission dynamics of schistosoma japonicum in the lakes and marshlands of china. | schistosoma japonicum is a major public health concern in china, with over one million people infected and another 40 million living in areas at risk of infection. unlike the disease caused by s. mansoni and s. haematobium, schistosomiasis japonica is a zoonosis, involving a number of different mammalian species as reservoir hosts. as a result of a number of published reports from china, it has long been considered that bovines, particularly water buffaloes, play a major role in human s. japonic ... | 2008 | 19115007 |
| first isolation of escherichia coli o157:h7 from faecal and milk specimens from anatolian water buffaloes (bubalus bubalus) in turkey. | three hundred rectal faecal samples and 213 raw milk samples obtained from the tanks and containers were examined using standard cultural methods. escherichia coli o157:h7 was isolated from 11 (3.7%) of 300 faecal samples and 3 (1.4%) of 213 raw milk samples. it was determined that 8 (73%) of e. coli o157:h7 strains isolated from faecal samples originated from water buffaloes younger than 2 years of age and 3 (27%) from 2-year-old and older water buffaloes. this is the 1st isolation of escherich ... | 2008 | 19496315 |
| phylogenetic analysis and comparison between cow and buffalo (including egyptian buffaloes) mitochondrial displacement-loop regions. | mitochondrial dna (mtdna) analysis has been used extensively for phylogenetic analysis studies and systematics. the displacement loop (d-loop) region inside the mtdna is a non-coding part whose analysis can indicate variations between closely related populations. this paper reports for the first time the characterization and analysis of the complete sequence of the d-loop region from egyptian buffaloes and analysis in conjunction with previously published indian and european bubalus bubalis and ... | 2008 | 19462514 |
| a proteomic characterization of water buffalo milk fractions describing ptm of major species and the identification of minor components involved in nutrient delivery and defense against pathogens. | water buffalo has been studied in relation to the exclusive use of its milk for the manufacture of high-quality dairy products. buffalo milk presents physicochemical features different from that of other ruminant species, such as a higher content of fatty acids and proteins. we report here a detailed proteomic analysis of buffalo skim milk, whey and milk fat globule membrane fractions. notwithstanding the poor information available on buffalo genome, identification of protein isoforms correspond ... | 2008 | 18668696 |
| relationship between peripheral plasma inhibin and fsh concentrations in sahiwal cows (bos indicus) and murrah buffaloes (bubalus bubalis) during estrous cycle. | the present investigation was undertaken to study the relationship between circulating inhibin and fsh concentrations during the estrous cycle in buffaloes and sahiwal cattle. the pattern of inhibin concentrations was similar, with peak concentrations on day -2 (day 0 = day of estrus) and minimum concentrations on days 12 and 11 in buffaloes and cattle, respectively. circulating fsh concentrations were the highest on day 0 and lowest on days 8 and 13 in buffaloes and cattle, respectively. periph ... | 2008 | 18575966 |
| cryptosporidiosis in buffalo calves (bubalus bubalis): prevalence and potential risk factors. | the objective of the present study was to describe the prevalence and risk factors associated with cryptosporidiosis in buffalo calves in middle egypt. during one year, 458 fecal samples were collected from buffalo calves less than 3 month age in 55 small scale herds and examined for the presence of cryptosporidium oocysts. data describing age, gender, season, and herd management practices were gathered to assess potential risk factors. fecal examination showed that 14.19% of the examined calves ... | 2008 | 18575969 |
| fatal intestinal coccidiosis in a three week old buffalo calf (bubalus bubalus). | the water buffalo (bubalus bubalus) is important to the economy of several countries, especially in asia and brazil. little is known of the impact of coccidiosis in buffaloes. cattle and buffaloes are considered to have common species of eimeria but critical cross transmissions have not been made because it is difficult to raise these hosts coccidian free. clinical coccidiosis was confirmed post mortem in a 22-day old buffalo calf that died after a 3-4 day illness. oocysts morphologically identi ... | 2008 | 18576888 |
| evaluation of a fluorescence polarization assay for the detection of serum antibodies to brucella abortus in water buffalo (bubalus bubalis). | the fluorescence polarization assay (fpa) was evaluated for the serological diagnosis of brucellosis in water buffalo (bubalus bubalis) in southern italy. this assay uses o-polysaccharide prepared from brucella abortus lipopolysaccharide conjugated with fluorescein isothiocyanate as a tracer. it has many methodological advantages over older, more established tests and can be performed in a fraction of the time. sera from 890 buffalos from the campania region - 526 positive sera and 364 negative ... | 2008 | 18599128 |
| experimental transmission of foot-and-mouth disease among indian buffalo (bubalus bubalis) and from buffalo to cattle. | indian buffalo and cattle were infected experimentally with a serotype o strain of foot-and-mouth disease virus of buffalo origin. whereas intradermolingual inoculation of buffalo produced largely sub-clinical infection, inoculation in the dental pad produced vesicles in the mouth and on the feet. a buffalo infected via the dental pad transmitted infection to cattle and buffalo by direct contact with them for 24h. the contact-exposed buffalo developed (1) delayed-onset clinical signs, and (2) sh ... | 2008 | 18619607 |
| expression profile of toll like receptors in a range of water buffalo tissues (bubalus bubalis). | the present study was carried out to determine the expression profile of toll-like receptors (tlrs) 1-10 in buffalo peripheral blood mononuclear cells (pbmnc), neutrophils, spleen, liver, lung, heart, kidney, ovary and uterus using reverse transcriptase polymerase chain reaction (rt-pcr) with bovine tlr-specific primers the buffalo tlr partial nucleotide sequences had 95-98% nucleotide homology with bovine tlr sequences available in the genbank. pbmnc expressed all tlrs except tlr1 and neutrophi ... | 2008 | 18619678 |
| effect of type of cryoprotectant on morphology and developmental competence of in vitro-matured buffalo (bubalus bubalis) oocytes subjected to slow freezing or vitrification. | the present study examined the effects of different cryoprotectants on morphology and developmental competence of in vitro-matured buffalo oocytes after slow freezing or vitrification. after slow freezing in dimethyl sulfoxide (dmso), ethylene glycol (eg) or 1,2-propanediol (proh), at 1.0 or 1.5 m each, the proportion of morphologically normal oocytes recovered was significantly higher (p < 0.05) with 1.5 than 1.0 m for all cryoprotectants and was highest (p < 0.05) for 1.5 m dmso. following vit ... | 2008 | 18462611 |