Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| isolation of chlamydia psittaci involved in abortion of goats in mexico: first report. | a group of 35 healthy adult goats ranging from two to six years old were bought from a chlamydiosis and brucellosis-free flock; they were vaccinated against brucella melitensis with rev1 vaccine at reduced doses, and one month later placed in isolation units. the animals were one month pregnant at the moment of purchase, and during the third month of pregnancy 10 out of the 35 dams aborted. necropsy of the aborted fetus and examination of the foetal membranes was performed where no macroscopic l ... | 1997 | 10932720 |
| in vitro activity of antibiotics and combinations against brucella melitensis. | 1997 | 11864168 | |
| immunological assay after vaccination of goats with brucella melitensis rev. i and brucella abortus 45/20 vaccines at saudi arabia. | the living smooth brucella melitensis rev. i vaccine given in the normal dose (7x10(8) organism) to goats 3 to 5 months of age stimulated a marked increase in agglutinin titer. fractionation of pools of serum by gel filtration by anion exchange chromatography, using diethylaminoethyl (deae -) cellulose showed that both igm and igg agglutinins were present from 12 to 47 days after goats were vaccinated. only mercaptoethanol (me)-sensitive agglutinins were detected in most goats 4 months after vac ... | 1997 | 17216995 |
| [epidemiological analysis on the rising of brucellosis prevalence in the area already under controlled]. | an epidemiological analysis was made on the outbreak of brucellosis in sui-de county, shaanxi province in 1995-1996. result showed that it was one of the most serious brucellosis outbreaks since 1986 when national brucellosis under controlled critiria had been met in the province, and the number of cases was continuously spreading and growing. the incidence was up to 84.8 per 10(5) and the prevalence rate reached 17.5 percent. patients had typical symptoms with bad conditions. most all the cases ... | 1997 | 9812474 |
| human infection by brucella melitensis: an outbreak attributed to contact with infected goats. | although several outbreaks of brucella melitensis infection have been reported among laboratory workers or goat cheese consumers, outbreaks related to rural labour have been rarely studied. an outbreak of human brucellosis among farm workers of argentina was studied and revealed a close relationship with an epidemic of caprine abortions which occurred shortly before on the same farm. high rates of b. melitensis infection were found among goats. active brucellosis was diagnosed in 33 subjects (14 ... | 1997 | 9537757 |
| [aneurysm of the thoracic aorta and spondylodiscitis disclosing brucellosis]. | brucellosis, which decreased during the eighties in france, remains a public health problem in many mediterranean countries. we report the case of a 65-year old patient native of morocco, of thoracic aorta aneurysm and lumbar spondylodiscitis due to brucella melitensis, revealed by haemoptysis and lumbar pains, with a favourable outcome after aortic graft resection, spinal plaster immobilization and specific lengthy antibiotic treatment. this case report is characterized by the absence of endoca ... | 1997 | 9499988 |
| mapping and identification of brucella melitensis proteins by two-dimensional electrophoresis and microsequencing. | two-dimensional (2-d) gel electrophoresis was used to map brucella melitensis proteins. the 2-d proteins map of b. melitensis b115 revealed 595 silver-stained protein spots separated by both isoelectric point and molecular mass. twenty-five proteins were identified either by immunoblotting using monoclonal antibodies (mabs) or by n-terminal microsequencing. the protein spots identified by mabs were the 89 kda outer membrane protein, dnak, bacterioferritin, cp24, and bp26. some spots were identif ... | 1997 | 9059838 |
| total knee arthroplasties infected by brucella melitensis: a case report. | a 74-year-old man with bilateral knee arthroplasties developed brucella arthritis in both joints after a total joint arthroplasty in his left knee. this complication is the second case reported in the literature to date. | 1997 | 9113296 |
| brucellosis in fish. | brucellosis is a zoonotic infectious disease that represents serious economic and health problems. it has been extensively studied in different species of livestock but literary references to fish brucellosis are scarce. therefore, the purpose of the research hereby presented was to investigate the serologic-bacteriologic response of nile catfish to experimental infection with brucellosis. thirty predatory catfishes were used. they were divided into two groups. the first was subjected to s. c. i ... | 1997 | 9123781 |
| characterization of smooth lipopolysaccharides and o polysaccharides of brucella species by competition binding assays with monoclonal antibodies. | previously, four epitope specificities on the o chain of brucella species were reported: m, a, c, and c/y. in this work, according to monoclonal antibody binding to smooth lipopolysaccharides of yersinia enterocolitica 0:9, brucella abortus w99 (a-dominant strain), and b. melitensis rev1 (m-dominant strain), seven o-chain epitope specificities were defined: m, a, c (m > a), c (m = a), c/y (m > a), c/y (m = a) and c/y (a > m). competitive binding assays between these monoclonal antibodies suggest ... | 1997 | 9125584 |
| brucella melitensis osteitis following craniotomy in a patient with aids. | 1997 | 9142817 | |
| antibody and delayed-type hypersensitivity responses to ochrobactrum anthropi cytosolic and outer membrane antigens in infections by smooth and rough brucella spp. | immunological cross-reactions between brucella spp. and ochrobactrum anthropi were investigated in animals and humans naturally infected by brucella spp. and in experimentally infected rams (brucella ovis infected), rabbits (brucella melitensis infected), and mice (b. melitensis and brucella abortus infected). in the animals tested, o. anthropi cytosolic proteins evoked a delayed-type hypersensitivity reaction of a frequency and intensity similar to that observed with b. melitensis brucellin. o. ... | 1997 | 9144364 |
| a clinical study of brucellosis in adults in the asir region of southern saudi arabia. | one hundred four saudi patients with brucellosis who were admitted to abha general hospital in the asir region of southern saudi arabia were studied. all the patients had brucella melitensis infection; 61.5% were females while 38.5% were males. their mean age was 32 years. most of the patients (61.5%) lived in the lowland (tihama) and the majority were shepherds (84.6%). the most common presenting symptoms were fever (100%), sweating (96.2%), headache (76.9%), joint pains (76.9%), and backache ( ... | 1997 | 9158043 |
| comparison of bactec 9240 peds plus medium and isolator 1.5 microbial tube for detection of brucella melitensis from blood cultures. | the sensitivity and time to detection of brucella melitensis by the bactec 9240 and the isolator blood culture systems were compared in a prospective volume-controlled study. blood sample aliquots, obtained from children with suspected brucellosis, were inoculated into a bactec 9240 peds plus bottle and into an isolator 1.5 microbial tube. overall, 122 pairs of blood samples for culture were obtained, and 28 (23%) were positive by at least one method. the bactec 9240 system detected all 28 posit ... | 1997 | 9163448 |
| serological serum protein fraction and responses to brucella melitensis in lambs fed aflatoxins. | twenty-four lambs were given 2 ppm aflatoxins (af) in their diet for 37 d and 12 were kept as a control group. after this time, toxic feed was removed for a further 35 days (clearance period). on day 17 all lambs were vaccinated with b melitensis strain rev-1, and blood samples were taken regularly to determine the levels of antibodies and serum proteins. aflatoxins decreased titers of rose bengal test and optical densities of elisa, both in the intoxicated and clearance periods. complement fixa ... | 1997 | 9167241 |
| lymphocyte proliferative responses of goats vaccinated with brucella melitensis 16m or a delta pure201 strain. | the response to a brucella melitensis purek deletion mutant, delta pure201 (referred to as strain 201), was compared with the response to its parental strain, 16m, in juvenile goats. proliferative responses to gamma-irradiated bacteria were detected earlier in strain 201-infected goats. lymphocytes from strain 16m- or 201-infected goats proliferated in response to one-dimensional polyacrylamide gel electrophoresis-separated proteins of similar mass isolated from strain 16m or brucella abortus rb ... | 1997 | 9199478 |
| transmission of brucella melitensis from sheep to lambs. | forty-one pregnant sheep showing positive immune responses to brucella melitensis in serological or allergic tests were selected from naturally infected flocks and kept in an isolated pen for lambing. the resulting 62 lambs were maintained in the same pen with their dams during lactation. when the lambs were weaned, the dams were slaughtered for bacteriological study and the lambs were reared in a clean pen. fourteen ewes excreted b melitensis during lactation and 17 were found to be infected po ... | 1997 | 9203318 |
| the economic impact of a bioterrorist attack: are prevention and postattack intervention programs justifiable? | understanding and quantifying the impact of a bioterrorist attack are essential in developing public health preparedness for such an attack. we constructed a model that compares the impact of three classic agents of biologic warfare (bacillus anthracis, brucella melitensis, and francisella tularensis) when released as aerosols in the suburb of a major city. the model shows that the economic impact of a bioterrorist attack can range from an estimated $477.7 million per 100,000 persons exposed (br ... | 1997 | 9204289 |
| brucellosis: an overview. | brucellosis remains a major zoonosis worldwide. although many countries have eradicated brucella abortus from cattle, in some areas brucella melitensis has emerged as a cause of infection in this species as well as in sheep and goats. despite vaccination campaigns with the rev 1 strain, b. melitensis remains the principal cause of human brucellosis. brucella suis is also emerging as an agent of infection in cattle, thus extending its opportunities to infect humans. the recent isolation of distin ... | 1997 | 9204307 |
| infection of a pacemaker by brucella melitensis. | we report a case that, to the best of our knowledge, is the only published instance of infection of a pacemaker and its leads by brucella melitensis. furthermore, this case suggests that b. melitensis may be able to persist around pacemaker devices despite its having been eliminated from the rest of the body. the patient was a sheep shearer who had just undergone a 45-day course of antibiotic therapy for brucella and had been considered cured on the basis of negative blood cultures. | 1997 | 9205989 |
| [15th international symposium of salmonellosis and brucellosis: a report]. | 1997 | 9221550 | |
| [the functional metabolic activity of the blood neutrophils in different clinical forms of brucellosis]. | the characteristics of the activity of alkaline phosphatase, nonspecific esterase, myeloperoxidase, lysosomal cation proteins, lipids have been determined in the cytochemical study of neutrophils in peripheral blood samples of 127 patients with acute, chronic, primary chronic brucellosis and osteochondrosis. it is expedient to use these characteristics as additional tests for the differential diagnostics of different clinical forms of brucellosis, as well as residual brucellosis and osteochondro ... | 1997 | 9221662 |
| a review of the use of b. melitensis rev 1 vaccine in adult sheep and goats. | the live brucella melitensis rev 1 strain is considered the best vaccine available for the prophylaxis of brucellosis in small ruminants. the classically recommended exclusive vaccination of young replacement animals has failed to control brucellosis in some developed countries and is frequently inapplicable in the developing world. accordingly, whole-flock vaccination is the only feasible alternative to control b. melitensis infection in small ruminants under the extensive management conditions ... | 1997 | 9234451 |
| serological findings obtained in cattle herds immunised with the brucella melitensis rev.1 and the b. abortus b19 vaccine in mongolia. | to reduce the economic losses caused by brucella abortus infection under the conditions of nomadic cattle breeding, more than half million cattle over 3 months of age (excluding male animals) were immunised with the b. melitensis rev.1 vaccine in 7 provinces of mongolia in the first year of a programme (1987). in the second year, only heifer calves over 3 months of age, yearling animals not vaccinated in the first year, as well as heifers over 2 years of age and cows were vaccinated. the vaccine ... | 1997 | 9270127 |
| identification of the major t-cell antigens present in the brucella melitensis b115 protein preparation, brucellergene ocb. | brucellergene is a commercial allergen prepared from brucella melitensis strain b115 and containing at least 20 cytoplasmic proteins. these proteins were separated by sds-page. the unstained gel was divided into 18 fractions and proteins were eluted from the gel fractions. the capacity of the separated proteins to elicit delayed-type hypersensitivity (dth) in infected guinea-pigs or to induce the production of interferon-gamma (ifn-gamma) by blood cells from infected cattle was evaluated. the bi ... | 1997 | 9291893 |
| localization and characterization of a specific linear epitope of the brucella dnak protein. | we have previously produced and characterized four monoclonal antibodies to the brucella dnak protein which were derived from mice infected with b. melitensis or immunized with the b. melitensis cell wall fraction. by use of a recombinant dna technique, we have localized a linear epitope, recognized by two of these monoclonal antibodies (v78/07b01/g11 and v78/09d04/d08), in the last 21 amino acids of the c-terminal region of the brucella dnak protein. the c-terminal region has been reported to b ... | 1997 | 9297829 |
| identification and characterization of brucella ovis immunogenic proteins using two-dimensional electrophoresis and immunoblotting. | in a previous report, proteins from brucella melitensis were characterized by two-dimensional polyacrylamide gel electrophoresis (2-d page) and n-terminal microsequencing. in the present report, we have extended this study to the second etiologic agent in ovine brucellosis, b. ovis, responsible for ram epididymitis and infertility. the combination of 2-d gel electrophoresis and protein microsequencing facilitated the location and identification of the major proteins of b. ovis on the 2-d pattern ... | 1997 | 9298663 |
| [fever, pulmonary infiltrates and hepatosplenomegaly]. | 1997 | 9304141 | |
| dna polymorphism at the omp-31 locus of brucella spp.: evidence for a large deletion in brucella abortus, and other species-specific markers. | the omp-31 gene, encoding a major outer-membrane protein in brucella melitensis, was pcr-amplified from brucella strains representing all species and known biovars by using primers selected according to the b. melitensis 16m omp-31 published sequence. amplification of omp-31 was achieved from dna of all brucella species with the exception of brucella abortus, the only brucella species where expression of omp-31 was not detected by reactivity with an mab specific for an epitope located in omp-31. ... | 1997 | 9308175 |
| survival of a bacterioferritin deletion mutant of brucella melitensis 16m in human monocyte-derived macrophages. | a bacterioferritin (bfr) deletion mutant of brucella melitensis 16m was generated by gene replacement. the deletion was complemented with a broad-host-range vector carrying the wild-type bfr gene, pbbr-bfr. the survival and growth of the mutant, b. melitensis pad 2-78, were similar to those of its parental strain in human monocyte-derived macrophages (mdm). these results suggest that bfr is not essential for the intracellular survival of b. melitensis in human mdm. | 1997 | 9317046 |
| scrotal gray-scale and color doppler sonographic findings in genitourinary brucellosis. | brucellosis is an enzootic disease that is frequently transmitted from brucella-infected animals and their products to humans through the gastrointestinal tract. genitourinary complications are seen in 2-10% of cases. we used sonography to investigate such complications in patients from a brucella-endemic area. | 1997 | 9321717 |
| rapid detection of brucella melitensis from blood cultures by a commercial system. | to assess the capability of the peds plus medium of the bactec 9240 blood culture system to recover brucella melitensis within the routine seven-day protocol used by most clinical microbiology laboratories, inoculated blood culture bottles were monitored by the bactec 9240 instrument for four weeks, and blind subcultures were performed once a week. a total of 2579 blood cultures were drawn, 42 (1.6%) of which were positive for brucella melitensis. forty-one of the 42 (97.6%) positive cultures we ... | 1997 | 9323475 |
| [fever and dry cough in a construction worker from portugal]. | a 33-year-old portugese worker presented with a one-week history of nonproductive cough and fever. a presumptive diagnosis "viral infection of the respiratory tract" was made. however, because of persisting cough and fever further investigations were necessary, and finally brucella melitensis was isolated in blood cultures. three months before admission to the hospital the man was dressing the carcasses of a goat in portugal and consumpted fresh goats milk cheese. antibiotic therapy with rifampi ... | 1997 | 9340710 |
| brucellar osteomyelitis involving prosthetic extra-articular hardware. | we report the case of a patient with brucella melitensis osteomyelitis involving non-joint prosthetic implant of the femur. this is the first case published of osteomyelitis by brucella sp. in a patient with prosthetic implant in bone and the second one with both intra- or extra-articular prosthetic bone implant. brucella melitensis is a rare organism which causes osteomyelitis in patients with prosthetic hardware, and should be added to the list of suspected organisms responsible for this disea ... | 1997 | 9354359 |
| the differentiation of brucella species by substrate specific tetrazolium reduction. | in the development of a tetrazolium reduction assay to replace substrate stimulated oxygen uptake for the identification of brucella species, nine tetrazolium salts were evaluated. only the more readily reduced compounds (mtt and int) detected increased metabolic activity with the more fastidious, slow growing strains and with b. suis strains on l-arginine and dl-ornithine. the assay was optimised with mtt. mtt reduction profiles offered with the medium on which the cells were grown. cells grown ... | 1997 | 9355260 |
| a 74-year-old man with persistent fevers. | an elderly man with a history of extensive world travel presents with a chronic illness and fevers. the febrile illness has been present for eight years, and no diagnosis has been made despite extensive evaluation and testing. the differential diagnosis of this unusual case of fever of unknown origin is discussed. | 1997 | 9392941 |
| detection and differentiation of the six brucella species by polymerase chain reaction. | brucelosis is a severe acute febrile disease caused by bacteria of the genus brucella. its current diagnosis is based on clinical observations that may be complemented by serology and microbiological culture tests; however, the former is limited in sensitivity and specificity, the latter is time consuming. to improve brucelosis diagnosis we developed a test which is specific and sensitive and is capable of differentiating the six species of brucella. | 1997 | 9407549 |
| a brucella melitensis high-temperature-requirement a (htra) deletion mutant is attenuated in goats and protects against abortion. | it has been previously demonstrated that a brucella melitensis high-temperature-requirement a (htra) deletion mutant is more susceptible to oxidative killing in vitro than the parental strain and is attenuated in mice. to evaluate the contribution of the b melitensis htra protease to virulence in ruminants, the capacity of the b melitensis htra mutant rwp5 to produce abortion in goats was compared to that of the virulent parental strain 16m. experimental infection with strain 16m caused abortion ... | 1997 | 9429252 |
| [spontaneous bacterial peritonitis caused by brucella]. | 1997 | 9441188 | |
| the characterisation of brucella strains isolated from marine mammals. | small gram negative coccobacilli isolated from seals, porpoises, dolphins and from an otter road casualty were identified as brucellae by their colonial and cell morphology, staining characteristics, biochemical activity, agglutination by monospecific antisera and susceptibility to lysis by brucella specific bacteriophage. their characterisation, including metabolic profiles, is described. these strains could not be assigned to recognised nomen species of the genus brucella and it is suggested t ... | 1997 | 9444074 |
| serological and bacteriological study of swine brucellosis. | a serological and bacteriological study was performed with sera taken from 2,228 swine from six states in venezuela. none of the animals were vaccinated against brucellosis, and the prevalence of the disease varied from 5 to 89% on farms located in these states. our studies indicated that the animals could be categorized into four groups depending on the degree of reactivity in serological tests. brucella suis biovar 1 was isolated from the lymph nodes, spleens, and semen samples of seropositive ... | 1997 | 8968931 |
| delayed-type hypersensitivity activity of the brucella l7/l12 ribosomal protein depends on posttranslational modification. | the ribosomal protein l7/l12 isolated from brucella melitensis induces a delayed-type hypersensitivity (dth) reaction in brucella-sensitized guinea pigs. surprisingly, the recombinant brucella l7/l12 protein expressed in escherichia coli as a fusion protein with a six-histidine tag cannot elicit such a reaction. the six histidines tagged to the recombinant l7/l12 protein were removed enzymatically, but the resulting protein did not induce a dth reaction in sensitized animals. incubation of the r ... | 1997 | 8975922 |
| congenital brucellosis in a premature infant. | 1997 | 9002111 | |
| characterization, occurrence, and molecular cloning of a 39-kilodalton brucella abortus cytoplasmic protein immunodominant in cattle. | monoclonal antibodies and polyclonal antisera recognizing a 39-kda protein (p39) of brucellin, a cytoplasmic extract from brucella melitensis rough strain b115, were produced. the p39 was purified by anion-exchange chromatography. eleven of fourteen brucella-infected cows whose infections had been detected by the delayed-type hypersensitivity (dth) test with brucellergen also developed a dth reaction when purified p39 was used as the trigger. the t-cell proliferative responses to p39 of peripher ... | 1997 | 9009303 |
| production and characterization of monoclonal antibodies specific for the lipopolysaccharide of escherichia coli o157. | identification of the o157 antigen is an essential part of the detection of escherichia coli o157:h7, which is recognized as a major etiologic agent of hemorrhagic colitis. however, polyclonal antibodies produced against e. coli o157:h7 lipopolysaccharide (lps) may react with several other bacteria including brucella abortus, brucella melitensis, yersinia enterocolitica o9, escherichia hermannii, and stenotrophomonas maltophilia. we produced eight monoclonal antibodies (mabs) specific for the lp ... | 1997 | 9041412 |
| [infectious-allergic ophthalmic brucellosis (the validation of a new nosological form)]. | the necessity of distinguishing a new nosological entity: infectious allergic ophthalmic brucellosis-is validated. based on her own clinical and experimental findings and published data, the author describes the etiology, pathogenesis, clinical picture, diagnosis, and treatment of this disease. | 1996 | 9045486 |
| [epidemic outbreak of 81 cases of brucellosis following the consumption of fresh cheese without pasteurization]. | in spite of the great effort that has been made in recent years in castilla-la mancha to control brucelosis, a lack of awareness on the part of producers and consumers leads to major epidemic outbreak, such as the one described below. | 1996 | 9005033 |
| competitive enzyme-linked immunosorbent assay using monoclonal antibodies to the brucella melitensis bp26 protein to evaluate antibody responses in infected and b. melitensis rev.1 vaccinated sheep. | competitive enzyme-linked immunosorbent assay (c-elisa) was performed using 15 monoclonal antibodies (mabs), specific for brucella bp26 (previously also called cp28), a periplasmic protein antigen, to investigate antibody responses in naturally and b. melitensis h38 experimentally infected and b. melitensis rev.1 vaccinated sheep. the antigen preparation consisted of cytosoluble protein extract (cpe) of b. melitensis b115. by combining the c-elisa results of several mabs, a high percentage of na ... | 1996 | 9008343 |
| detection of brucella melitensis by bactec nr 730 and bactec 9120 systems. | in many parts of the world, brucellosis has significantly decreased, but it is still a problem in some regions of turkey. in our laboratory we have isolated 58 brucella spp. through bactec nr 730 and 30 brucella spp. through bactec 9120 systems. 31 of the 58 isolates were detected by bactec nr 730 system in 72 hours. the majority of the growth values were between 45-65. bactec 9120 system detected all the isolates in 84 hours. | 1996 | 8982628 |
| brucella melitensis--a sexually transmissible agent? | 1996 | 8656919 | |
| brucella melitensis biotype 1 outbreak in goats in northern kwazulu-natal. | 1996 | 8786610 | |
| effect of antibiotics contained in two brucella selective media on growth of brucella abortus, b. melitensis, and b. ovis. | the mic and the highest concentration enabling bacterial growth (ceg) of the antibiotics contained in two selective media were determined for brucella abortus, b. melitensis, and b. ovis. the nalidixic acid and bacitracin contained in farrell's selective medium were responsible for the inhibitory effects observed. | 1996 | 8789029 |
| polymorphism at the dnak locus of brucella species and identification of a brucella melitensis species-specific marker. | the dnak gene and surrounding sequences from reference strains of the six brucella species were amplified by the polymerase chain reaction (pcr) with primers chosen according to the published sequence of the b. ovis dnak gene and studied for polymorphism with nine restriction endonucleases. the restriction patterns were identical for all species with all restriction endonucleases tested except for b. melitensis strain 16m that showed a different pattern with ecorv, consistent with the presence o ... | 1996 | 8810947 |
| production and characterisation of monoclonal antibodies to brucella melitensis cytosoluble proteins that are able to differentiate antibody responses of infected sheep from rev. 1 vaccinated sheep. | monoclonal antibodies (mabs) were produced to brucella melitensis cytosoluble proteins (cp) with apparent molecular masses of 12, 24 and 28 kda (cp12, cp24 and cp28) which were previously shown by immunoblotting to differentiate antibody responses of infected sheep from those of b. melitensis strain rev. 1 vaccinated sheep. these mabs were derived from mice infected with virulent smooth (s) b. melitensis strain h38. most mabs obtained were directed to cp28, which indicated (as was shown in infec ... | 1996 | 8810948 |
| thrombosis of the abdominal aorta secondary to brucella spondylitis. | spondylitis is one of the most frequent osteoarticular complications of brucella infection in adults. occasionally it gives rise to soft tissue paravertebral or epidural masses, which can compress surrounding structures. a patient with thrombosis of the abdominal aortic artery secondary to brucella lumbar spondylitis is presented here, a complication that has not been previously reported. | 1996 | 8811368 |
| brucella infection of the thyroid gland. | three cases of brucella infection of the thyroid gland are reported. all three were female, and two were from rural areas in tehran province. all presented with fever, chills, and painful swelling in the neck, had positive wright and 2-mercaptoethanol test; and had normal thyroid function. cultures of fna materials grew brucella in all cases. they were cured following antibiotic treatment. | 1996 | 8936672 |
| outer membrane protein of neisseria meningitidis as a mucosal adjuvant for lipopolysaccharide of brucella melitensis in mouse and guinea pig intranasal immunization models. | a mucosal vaccine against brucellosis consisting of the lipopolysaccharide (lps) of brucella melitensis complexed with the outer membrane protein (gbomp) of group b neisseria meningitidis was tested in small-animal models of intranasal immunization. mice given two doses of the vaccine developed high levels of immunoglobulin g (igg) and iga antibodies specific for b. melitensis lps in lung lavages and specific igg and iga antibody-secreting cells in the lungs and spleen. similarly, in guinea pigs ... | 1996 | 8945575 |
| breast abscess caused by brucella melitensis. | cutaneous and soft tissue lesions are uncommon manifestations of brucellosis. though breast involvement in animal brucellosis is not uncommon, involvement of the breast in human brucellosis is extremely rare. we report a case of breast abscess in a 39-year-old female caused by brucella melitensis. treatment with combination of trimethoprim/sulphamethoxazole (tmp/ smx; cotrimoxazole) and doxycycline for 3 months resulted in clinical cure. | 1996 | 8945713 |
| recombinant brucella abortus proteins that induce proliferation and gamma-interferon secretion by cd4+ t cells from brucella-vaccinated mice and delayed-type hypersensitivity in sensitized guinea pigs. | optimal protective immunity to brucella abortus infection is dependent on a coordinate interaction between different t-cell subsets which leads to an antigen-specific t-lymphocyte-mediated activation of macrophages, the main cellular reservoir for the bacterium. as an initial step in the identification of bacterial proteins that mediate cellular immunity, we have subcloned the b. abortus ssb, uvra, groes, and groel genes into the prokaryotic expression vector pmal-c2 using pcr. escherichia coli ... | 1996 | 8964089 |
| agglutinins for brucellae antigens in blood sera of an urban and rural population in kenya. | the antibody titres for brucella arbotus and br. melitensis in 364 sera from healthy individuals in nairobi and naivasha are presented. a majority (96%) had no detectable agglutinins. reactivity was markedly higher in the naivasha serum samples, than in those from nairobi. in naivasha, seven per cent showed reactivity, whereas in nairobi a larger majority (98%), showed no reactivity, with antibody titres ranging from 1:20-1:160. age and sex were found to have no effect on antibody titre distribu ... | 1996 | 8698024 |
| bacterial persistence and immunity in goats vaccinated with a pure deletion mutant or the parental 16m strain of brucella melitensis. | to evaluate host responses, young goats were inoculated subcutaneously with a genetic deletion mutant (deltapure201) of brucella melitensis (n = 6), its virulent parental strain 16m (n = 6), or saline (n = 6). no clinical evidence of brucellosis was seen in any goat. serum antibody titers peaked at postinoculation day (pid) 14. bacteria in lymph nodes that drained sites of vaccination reached peak numbers of >10(6) cfu/g in both infected groups at pid 7 and progressively declined to pid 84. at n ... | 1996 | 8698463 |
| cloning of a brucella melitensis group 3 antigen gene encoding omp28, a protein recognized by the humoral immune response during human brucellosis. | brucella group 3 antigens (ags) are outer membrane proteins (omps) with a molecular mass ranging from 25 to 30 kda. the omps are of interest partially because of their potential use as vaccine and diagnostic reagents. we used human convalescent antibody (ab) to clone a gene that encoded a 28-kda protein from a lambdagt11 library of brucella melitensis 16m genomic dna. dna sequence analysis revealed a single open reading frame that would encode a protein of 26,552 da. the 28-kda protein had a pri ... | 1996 | 8698471 |
| complications associated with brucella melitensis infection: a study of 530 cases. | we carried out a prospective study of 530 patients older than 14 years of age with brucellosis. we describe the incidence and clinical features of the focal forms of the disease, analyzing some of the possible factors associated with their appearance. one hundred sixty-nine patients (31.9%) had a focal form or complication. osteoarticular complications were the most frequent, totaling 113 cases (66%), followed by genitourinary with 18 cases (5.1% of males), hepatic (2.5%), neurologic (1.7%), and ... | 1996 | 8699960 |
| enzyme-linked immunosorbent assay with partially purified cytosoluble 28-kilodalton protein for serological differentiation between brucella melitensis-infected and b. melitensis rev.1-vaccinated sheep. | the problem of differentiating sheep infected with brucella melitensis from those vaccinated or exposed to cross-reaching organisms has not been resolved by conventional serological tests or through the use of the smooth lipopolysaccharide in primary binding assays. we therefore analyzed sera from ewes experimentally infected with b. melitensis h38, from ewes naturally infected with b. melitensis, and from b. melitensis rev.1-vaccinated ewes by enzyme-linked immunosorbent assay with three antige ... | 1996 | 8705674 |
| a comparison of the potency of several brucella allergens used to detect brucellosis in cattle. | the potency of brucella allergens prepared from a smooth brucella abortus strain s-99, mucoid strain leewarden, rough strain 45/20, and rough brucella melitensis strain b-115 was assessed. the potency of these allergens was compared with that of a standard allergen prepared from smooth brucella abortus s-99 that efficiently detected bovine brucellosis in other studies. eight cattle experimentally inoculated with brucella abortus 544 were tested with the allergens 4 and 10 weeks after infection, ... | 1996 | 8711894 |
| protection of balb/c mice against homologous and heterologous species of brucella by rough strain vaccines derived from brucella melitensis and brucella suis biovar 4. | to evaluate stable rough mutants derived from brucella melitensis 16m and b suis 2579 (biovar 4) as vaccines against homologous and heterologous brucella spp in the balb/c mouse model. design, animals, and procedure: rough mutants vtrm1 and vtrs1 were obtained from b melitensis 16m and b suis 2579, respectively, by allelic exchange of rfbu gene encoding mannosyltransferase with a tn5-disrupted rfbu gene. mice were vaccinated with vtrm1 or vtrs1 and challenge exposed 8 weeks later. | 1996 | 8723881 |
| comparison of two selective media for the isolation of brucella melitensis from naturally infected sheep and goats. | the efficacy of two selective media for the isolation of brucella melitensis from naturally infected sheep and goats was compared. two hundred and eighty sheep and 60 goats belonging to b melitensis-infected flocks were slaughtered and samples of milk (when available) and seven tissues were taken from each animal for bacteriological analyses. a modified thayer-martin's medium was more sensitive than farrell's medium; by the combined use of both media 142 infected sheep and 40 infected goats were ... | 1996 | 8733178 |
| childhood brucellosis in north-western greece: a retrospective analysis. | fifty-two cases of childhood brucellosis which occurred in north-western greece during the 15-year period 1979-1993, are reviewed. it is believed that they represent very closely the total incidence of the disease in the region which has a population of 100,000 children aged 0-14 years old. brucellosis-affected children were almost exclusively from goat- or shepherd families and of both sexes and all age groups. a broad spectrum of clinical manifestations ranging from malaise only to brain absce ... | 1996 | 8750800 |
| cloning, nucleotide sequence, and expression of the brucella melitensis omp31 gene coding for an immunogenic major outer membrane protein. | the gene coding for the major outer membrane protein (omp) of 31 to 34 kda, now designated omp31, of brucella melitensis 16m was cloned and sequenced. a b. melitensis 16m genomic library was constructed in lambda gem-12 xhoi half-site arms, and recombinant phages expressing omp31 were identified by using the anti-omp31 monoclonal antibody (mab) a59/10f09/g10. subcloning of insert dna from a positive phage into pgem-7zf allowed the selection of a plasmid bearing a 4.4-kb ecori fragment that seeme ... | 1996 | 8751924 |
| overproduction of the brucella melitensis heat shock protein dnak in escherichia coli and its localization by use of specific monoclonal antibodies in b. melitensis cells and fractions. | the brucella melitensis dnak gene was amplified by the polymerase chain reaction using primers chosen according to the published sequence of b. ovis and cloned in multiple copy plasmids enabling expression under the control of the plac promoter. monoclonal antibodies (mab) obtained by immunizing mice with b. melitensis b115 cell wall (cw) fraction or by infecting mice with virulent b. melitensis strain h38 and recognizing a 73-kda band in immunoblotting of the b. melitensis cw fraction reacted w ... | 1996 | 8761733 |
| cloning, nucleotide sequence, and expression of the brucella melitensis bp26 gene coding for a protein immunogenic in infected sheep. | we have previously identified a brucella melitensis 28 kda cytosoluble protein (cp28) which was highly immunogenic in infected sheep and which in addition made possible the serological differentiation between infected and b. melitensis rev. 1 vaccinated sheep. monoclonal antibodies against cp28 were used to screen a b. melitensis 16m genomic library and to clone the corresponding gene. dna sequencing of the gene encoding cp28 of b. melitensis 16m revealed that it was nearly identical to that of ... | 1996 | 8764475 |
| [a seroepidemiological study of brucellosis infection in the province of campobasso]. | these research regarded a seroepidemiological study of brucellosis in the campobasso province. the results related to 204 subjects (96 males and 108 females) pointed out the 17.15% of subjects were positive for antibodies anti-brucella, 28.6% of these were positive for br. melitensis. the results agree with the data of the literature. | 1996 | 8766351 |
| brucella melitensis can be transmitted sexually. | 1996 | 8774592 | |
| infective endocarditis and occult splenic abscess caused by brucella melitensis infection: a case report and review of the literature. | this report describes a patient with prosthetic aortic valve endocarditis caused by brucella melitensis infection with an initial relapse after apparent recovery due to associated occult splenic abscess. in spite of a prolonged period of recovery (6 months) achieved with splenectomy and antibrucella antimicrobial therapy, a second relapse occurred and cure was achieved after replacement of the infected prosthetic valve and prolonged courses of antibrucella antimicrobial therapy. there is controv ... | 1996 | 8817011 |
| infected abdominal aortic aneurysm caused by spinal brucellar infection. | 1996 | 8819420 | |
| childhood brucellosis in israel. | brucellosis has become a major medical problem in israel particularly in the muslim arab population. | 1996 | 8823856 |
| first report of a strain of brucella melitensis that was widely sensitive to brucellaphages isolated in the united arab emirates. | 1996 | 8825005 | |
| inadequate azithromycin activity against brucella melitensis in mice with acute or chronic infections. | the activities of therapeutic regimens with azithromycin (azi) and doxycycline combined with streptomycin (dox-sm) were compared in brucella melitensis infected mice. in a chronic model, azi given over 10, 14 or 21 consecutive days (50 mg/kg/24 h) significantly reduced the infection (1.3-1.6 logs, day 48 post-infection). however, the effectiveness of dox (21 days, 50 mg/kg/12 h) was higher than azi (3.4 logs of reduction, day 48 post-infection). besides, when dox was administered for 45 days, it ... | 1996 | 8835110 |
| chronic subdural empyema: a new presentation of neurobrucellosis. | 1996 | 8842288 | |
| serological follow-up of human brucellosis by measuring igg antibodies to lipopolysaccharide and cytoplasmic proteins of brucella species. | a serological 2-year follow-up of 35 patients who had brucellosis with different clinical outcomes was performed. patients were followed up by standard tube agglutination (sta) and 2-mercaptoethanol sta (2me-sta) tests and by two enzyme-linked immunosorbent assays (elisas) to independently measure values of serum igg to either lipopolysaccharide (lps) or cytoplasmic proteins of brucella. whereas sta and 2me-sta tests revealed characteristic antibody profiles for patients who recovered (group i), ... | 1996 | 8852961 |
| hepatosplenic abscesses due to brucella melitensis: report of a case involving a child and review of the literature. | suppurative disease of the liver and/or spleen is a rare and serious complication of human brucellosis. in the english-language literature, only nine cases have been reported, all involving adults with chronic infection. we report the case of a young child in whom abscesses of the liver and spleen developed during acute brucellosis. brucella melitensis was cultured from an aspirate of the liver and from the bone marrow. after percutaneous drainage of the liver abscess, the patient responded to a ... | 1996 | 8852967 |
| brucella melitensis biotype 1 outbreak in goats in northern kwazulu-natal. | brucella melitensis biotype 1 was confirmed in indigenous, outbred goats in three northern districts of the kwazulu-natal province following the diagnosis of human malta fever in the same area. six foci of infection were found during an extensive serological survey involving 6266 goats carried out in most of the districts of the kwazulu-natal province. the prevalence in the positive herds varied between 17% and 100%. the diagnosis was confirmed by culturing milk samples from serologically positi ... | 1996 | 8856768 |
| surgical treatment of brucella endocarditis. | between april 1987 and october 1992, six cases of brucella endocarditis were operated on in the authors' hospital for valve replacement. they were five men and one woman with a mean(s.d.) age of 52(15) years (range 30-71 years). three patients were in new york heart association (nyha) class iii and three in class iv. two patients had previous history of rheumatic fever, one was a drug abuser, two had peripheral embolism and one constrictive pericarditis. most were living in rural areas. echocard ... | 1996 | 8861443 |
| cloning, nucleotide sequence, and expression of the gene coding for a ribosome releasing factor-homologous protein of brucella melitensis. | the gene coding for a brucella melitensis cytosoluble protein (cp24) that is immunogenic in infected sheep and a major component of brucellin inra was cloned and sequenced. as in brucella cells, cp24 was located in the cytoplasm of recombinant escherichia coli. the amino acid sequence predicted from the cloned gene revealed 48 and 46% identity with the ribosome releasing factor, a protein factor required for release of the 70s ribosome from the mrna, of e. coli and haemophilus influenzae rd, res ... | 1996 | 8890247 |
| molecular characterization, occurrence, and immunogenicity in infected sheep and cattle of two minor outer membrane proteins of brucella abortus. | screening of a brucella abortus genomic library with two sets of monoclonal antibodies allowed the isolation of the genes corresponding to two minor outer membrane proteins (omp10 and omp19) found in this bacterial species. sequence analysis of the omp10 gene revealed an open reading frame capable of encoding a protein of 126 amino acids. the nucleotide sequence of the insert producing the omp19 protein contains two overlapping open reading frames, the largest of which (177 codons) was shown to ... | 1996 | 8557326 |
| characterization of brucella abortus and brucella melitensis native haptens as outer membrane o-type polysaccharides independent from the smooth lipopolysaccharide. | brucella native haptens (nhs) extracted with hot water from smooth (s)-type b. abortus and b. melitensis were purified to high levels of serological activity and compared with the polysaccharide obtained by acid hydrolysis (ps) of the s lipopolysaccharide (s-lps). by 13c nuclear magnetic resonance analysis, nhs showed the spectrum of a homopolymer of alpha-1,2- or alpha-1,2- plus alpha-1,3-linked 4-formamido-4,6-dideoxy-d-mannose (n-formylperosamine) previously reported for the lps o chain. howe ... | 1996 | 8576040 |
| [brucella pancarditis with fatal outcome]. | brucella endocarditis was diagnosed in a 21-year-old itinerant farm worker hospitalized for acute pulmonary edema. history taking revealed cough, fever and sweating one month earlier which had been treated with antibiotics. at admission, echography showed lesions on the aortic valve and hemocultures identified brucella meltensis. on day 7 of specific treatment with doxycycline (200 mg/day) and rifamycine (1200 mg/day), and despite digitalics and diuretics, left ventricular failure rapidly worsen ... | 1996 | 8668692 |
| nucleotide sequence and expression of the gene encoding the major 25-kilodalton outer membrane protein of brucella ovis: evidence for antigenic shift, compared with other brucella species, due to a deletion in the gene. | the nucleotide sequences encoding the major 25-kda outer membrane protein (omp) (omp25 genes) of brucella ovis 63/290, brucella melitensis 16m, brucella suis 1330, brucella canis rm6/66, and brucella neotomae 5k33 (all reference strains) were determined and compared with that of brucella abortus 544 (p. de wergifosse, p. lintermans, j. n. limet, and a. cloeckaert, j. bacteriol. 177:1911-1914, 1995). the major difference found was between the omp25 gene of b. ovis and those of the other brucella ... | 1996 | 8675306 |
| deletion of pure attenuates brucella melitensis infection in mice. | we previously showed that a pure mutant (delta pure201) of brucella melitensis 16m is attenuated for growth in cultured human monocytes (e. s. drazek, h. h. houng, r. m. crawford, t. l. hadfield, d. l. hoover, and r. l. warren, infect. immun. 63:3297-3301, 1995). to determine if this strain is attenuated in animals, we compared the growth of the delta pure201 mutant with that of strain 16m in balb/c mice. the number of bacteria in the spleen and spleen weight peaked for both strains between 1 an ... | 1996 | 8675325 |
| [historical brief on the fever of malta]. | malta fever (brucellosis) is an infectious disease of cattle (chiefly goats) which can infect man. although the disease is not limited to malta, occurring also in other mediterranean countries as well as in asia and america, it was originally described in 1863 in malta where the responsible bacteria (brucella melitensis) was discovered in 1887 by d. bruce. ten years later wright & smith (1897) showed that the agglutination test originally established for typhoidic patients could be used for the ... | 1996 | 11624839 |
| a case of brucella melitensis endocarditis successfully treated by a combination of surgical resection and antibiotics. | endocarditis is a rare complication of brucellosis, often fatal, and the treatment is controversial. we present a case of brucella melitensis endocarditis successfully treated by a combination of surgical resection and antibiotics. | 1995 | 8685649 |
| brucellosis associated with unpasteurized milk products abroad. | 1995 | 8519642 | |
| immunological response in mice after long-term stimulation with cell wall antigens from brucella melitensis. | the continuous stimulation of the immune system using cell wall antigens from brucella melitensis was found to cause both quantitative and qualitative changes in circulating lymphocyte populations in mice. animals were inoculated in the hind legs with antigens on alternate days for varying lengths of time. during a two-month period, we saw a higher number of circulating lymphocytes, with an increase in the number of cd4+ cells (l3t4+) and b lymphocytes (i-ad). after two months, a drop in the ove ... | 1995 | 8525067 |
| brucellar bacteremia in pregnancy. | 1995 | 8527594 | |
| brucellosis presenting with cough. | 1995 | 8532438 | |
| [azithromycin, pharmacodynamic evaluation in animal models]. | several experimental models have been used in order to evaluate the in vivo efficacy of azithromycin against numerous human pathogenic bacteria and parasites, including comparison between azithromycin and other antibiotics belonging or not to the macrolide family. using the experimental models, three major objectives can be distinguished: the comparative studies of the efficacy dose 50 (ed50) of azithromycin compared to other orally given antibiotics, the azithromycin efficacy in animal infected ... | 1995 | 8539074 |
| doxycycline-rifampin versus doxycycline-streptomycin in treatment of human brucellosis due to brucella melitensis. the gecmei group. grupo de estudio de castilla-la mancha de enfermedades infecciosas. | brucellosis is a common zoonosis in many parts of the world; the best regimen for the treatment of brucellosis has not been clearly determined. we have carried out a multicenter, open, controlled trial in five general hospitals in spain to compare the efficacy and safety of doxycycline and rifampin (dr) versus doxycycline and streptomycin (ds) for the treatment of human brucellosis. the study included 194 ambulatory or hospitalized patients with acute brucellosis, without endocarditis or neurobr ... | 1995 | 8540716 |
| acute pancreatitis associated with brucellosis. | acute pancreatitis developed in a patient with acute brucellosis due to brucella melitensis. to the best of our knowledge this is only the second reported case of acute pancreatitis associated with brucella infection. | 1995 | 8580415 |
| single-step pcr for detection of brucella spp. from blood and milk of infected animals. | a versatile method for the extraction of brucella dna and pcr are presented as reliable tools for the detection of brucella spp. from body fluids of infected animals. two oligonucleotides homologous to regions of the gene encoding for an outer membrane protein (omp-2) were designed to detect the pathogen from milk and/or blood of infected goats, bovines, and human patients. the sensitivity of our test and its ability to detect the pathogen in samples from the field reveal a promising advance in ... | 1995 | 8586678 |
| [the epidemiological characteristics of brucellosis in the region of the northern caucasus]. | at present, the northern caucus region is the most unfavourable brucellosis area of the russian federation where 43.5% of the total russian incidence of this infection was recorded in 1993. over the past years (1988-1993) there was a highly unsteady-state improvement of the epidemiological situation, however, the rates of human morbidity with this infection greatly lag behind those in russia. the active foci of small cattle brucellosis as the leading source of human contamination with brucellosi ... | 1995 | 8587517 |