Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| cxcl10/cxcr3-mediated responses promote immunity to respiratory syncytial virus infection by augmenting dendritic cell and cd8(+) t cell efficacy. | the induction of inflammatory cytokines during respiratory viral infections contributes to both disease pathogenesis and resolution. the present studies investigated the role of the chemokine cxcl10 and its specific receptor, cxcr3, in the host response to pulmonary respiratory syncytial virus (rsv) infection. antibody-mediated neutralization of cxcl10 resulted in a significant increase in disease pathogenesis, including airway hyperresponsiveness (ahr), mucus gene expression, and impaired viral ... | 2008 | 18624292 |
| vaccination of calves using the brsv nucleocapsid protein in a dna prime-protein boost strategy stimulates cell-mediated immunity and protects the lungs against brsv replication and pathology. | respiratory syncytial virus (rsv) is a major cause of respiratory disease in both cattle and young children. despite the development of vaccines against bovine (b)rsv, incomplete protection and exacerbation of subsequent rsv disease have occurred. in order to circumvent these problems, calves were vaccinated with the nucleocapsid protein, known to be a major target of cd8(+) t cells in cattle. this was performed according to a dna prime-protein boost strategy. the results showed that dna vaccina ... | 2008 | 18644416 |
| chronological and clinical characteristics of apnea associated with respiratory syncytial virus infection: a retrospective case series. | infants with respiratory syncytial virus (rsv) infection are at risk for developing apnea. the authors compared patients with rsv infection who develop apnea with those who do not, to help ambulatory physicians risk stratify their patients with rsv infection. | 2008 | 18648081 |
| epidemiologic characteristics and seasonal distribution of human metapneumovirus infections in five epidemic seasons in stockholm, sweden, 2002-2006. | the presence of human metapneumovirus (hmpv) was analyzed retrospectively by reverse transcriptase-polymerase chain reaction (rt-pcr) in five epidemic seasons, in stockholm, 2002-2006. the occurrence of hmpv was compared with five common respiratory viruses; respiratory syncytial virus, influenza a virus, influenza b virus, parainfluenza virus and adenovirus. with a detection rate of 2.9% (n = 143/4,989) in nasopharyngeal samples over the whole period, hmpv was the fourth most common respiratory ... | 2008 | 18649344 |
| th1 and th2 cytokine levels in nasopharyngeal aspirates from children with human bocavirus bronchiolitis. | human bocavirus (hbov) is regarded as one of the possible etiologic agents in lower respiratory tract infection and bronchial asthma exacerbation in children despite frequent co-detection with other respiratory viruses. the immunologic response in children with hbov infection is still not clear. | 2008 | 18650126 |
| detection of 11 common viral and bacterial pathogens causing community-acquired pneumonia or sepsis in asymptomatic patients by using a multiplex reverse transcription-pcr assay with manual (enzyme hybridization) or automated (electronic microarray) detection. | community-acquired pneumonia (cap) and sepsis are important causes of morbidity and mortality. we describe the development of two molecular assays for the detection of 11 common viral and bacterial agents of cap and sepsis: influenza virus a, influenza virus b, respiratory syncytial virus a (rsv a), rsv b, mycoplasma pneumoniae, chlamydophila pneumoniae, legionella pneumophila, legionella micdadei, bordetella pertussis, staphylococcus aureus, and streptococcus pneumoniae. further, we report the ... | 2008 | 18650351 |
| strategies for preventing respiratory syncytial virus. | prevention of respiratory syncytial virus (rsv) infection-crucial for decreasing the burden associated with this disease-is discussed. | 2008 | 19020197 |
| hospital pharmacists' role in the prevention and management of respiratory syncytial virus. | the role pharmacists play in managing respiratory syncytial virus (rsv) is discussed. | 2008 | 19020199 |
| impact of respiratory syncytial virus in the united states. | respiratory syncytial virus (rsv) infection is most common in infants and young children, with almost all children experiencing at least one infection by their second birthday. | 2008 | 19020201 |
| identification and management of severe respiratory syncytial virus. | identifying children at risk for severe respiratory syncytial virus (rsv) so that the most appropriate management strategies can be implemented, thereby reducing the morbidity associated with this disease, is discussed. | 2008 | 19020202 |
| innate immune response and bronchiolitis and preschool recurrent wheeze. | bronchiolitis and preschool recurrent wheeze (psrw) are common paediatric problems causing significant morbidity and mortality in the first years of life. respiratory syncytial virus (rsv) and rhinoviruses are the commonest pathogens associated with these illnesses. why some infants are severely affected, requiring admission to hospital, whilst others experience a simple cold is not fully understood: research has suggested that the innate immune response to these viruses is important. the innate ... | 2008 | 19026366 |
| identification of antibody neutralization epitopes on the fusion protein of human metapneumovirus. | human metapneumovirus (hmpv) is genetically related to respiratory syncytial virus (rsv); both cause respiratory tract illnesses ranging from a mild cough to bronchiolitis and pneumonia. the f protein-directed monoclonal antibody (mab) palivizumab has been shown to prevent severe lower respiratory tract rsv infection in animals and humans. we have previously reported on a panel of mabs against the hmpv f protein that neutralize hmpv in vitro and, in two cases, in vivo. here we describe the gener ... | 2008 | 19008400 |
| crystallization and preliminary x-ray analysis of the human respiratory syncytial virus nucleocapsid protein. | human respiratory syncytial virus (hrsv) has a nonsegmented negative-stranded rna genome which is encapsidated by the hrsv nucleocapsid protein (hrsvn) that is essential for viral replication. hrsv is a common cause of respiratory infection in infants, yet no effective antiviral drugs to combat it are available. recent data from an experimental anti-hrsv compound, rsv-604, indicate that hrsvn could be the target site for drug action. here, the expression, purification and preliminary data collec ... | 2008 | 18997331 |
| potential anti-respiratory syncytial virus lead compounds from aglaia species. | although the global prevalence of respiratory syncytial virus (rsv) infection, especially among infants and young children is on the increase, there are only limited therapeutic options for treatment of this disease. therefore, the search for novel antiviral inhibitors of rsv has become more intensive. in a pilot screening of eighteen compounds from various aglaia species for anti-rsv activity, we identified dammarenolic acid (ignt1), aglaiol (dupt1) and niloticin (cuct1) as potential anti-rsv c ... | 2008 | 18972843 |
| new strategies for control of respiratory syncytial virus infection. | to report on recent progress on the development and implementation of a vaccine against respiratory syncytial virus (rsv), on investigations of the mechanism of action of prophylactic antibodies and the potential for increased efficacy of those antibodies, and on candidate antiviral drugs against rsv. | 2008 | 18978532 |
| macrophage impairment underlies airway occlusion in primary respiratory syncytial virus bronchiolitis. | although respiratory syncytial virus (rsv) infection is the most important cause of bronchiolitis in infants, the pathogenesis of rsv disease is poorly described. we studied histopathologic changes in a panel of lung tissue specimens obtained from infants with fatal cases of primary rsv infection. in these tissues, airway occlusion with accumulations of infected, apoptotic cellular debris and serum protein was consistently observed. similar observations were found after rsv infection in new zeal ... | 2008 | 18980502 |
| cutting edge: eosinophils do not contribute to respiratory syncytial virus vaccine-enhanced disease. | respiratory syncytial virus (rsv) infection of balb/c mice previously immunized with a recombinant vaccinia virus (vacv) expressing the attachment (g) protein of rsv (vacvg) results in pulmonary eosinophilia, which mimics the response of formalin-inactivated rsv-vaccinated children, as well as increased weight loss, clinical illness, and enhanced pause (penh). we show that rsv infection of eosinophil-deficient mice previously immunized with vacvg results in the development of increased weight lo ... | 2008 | 18981084 |
| use of the seeplex rv detection kit for surveillance of respiratory viral outbreaks in toronto, ontario, canada. | the seeplex rv detection kit was used to identify specific respiratory viruses from specimens collected during respiratory outbreaks in the greater toronto area from 1 september 2007 to 1 february 2008. two hundred-thirty-one patient samples (nasopharyngeal swabs) were collected from 63 respiratory outbreaks. the distribution of outbreaks characterized by molecular means was: 30% (n=19) no identification; 52.5% (n=33) one pathogen; 14.5% (n=9) two pathogens; and 3% (n=2) three pathogens. in cont ... | 2008 | 18988931 |
| a novel active respiratory syncytial virus surveillance system in the united states: variability in the local and regional incidence of infection. | to characterize the onset, peak, and duration of the rsv season in major metropolitan areas in the united states as determined from laboratory test data collected by a novel rsv surveillance program (rsv alert), including regional and national trends. | 2008 | 18989237 |
| respiratory syncytial virus glycoproteins uptake occurs through clathrin-mediated endocytosis in a human epithelial cell line. | cell-surface viral proteins most frequently enter the cell through clathrin or caveolae endocytosis. respiratory syncytial virus antigen internalization by immune cells is via caveolin, however, uptake of paramyxovirus cell membrane proteins by non-immune cells is done through clathrin-coated pits. in this work, the uptake of respiratory syncytial virus cell surface glycoproteins by non-immune human epithelial cells was investigated through indirect immunofluorescence with polyclonal anti-rsv an ... | 2008 | 18950517 |
| wheezing illness caused by respiratory syncytial virus and other agents. | respiratory syncytial virus (rsv) and other pathogenic agents cause lower respiratory infection with wheezing in infants (wheezing illness in infancy). wheezing illness in infancy due to rsv can be life-threatening and can induce recurrent wheezing; but these events can also be produced by infection by other pathogenic agents. thus, whether rsv induces more severe wheezing illness in infancy remains poorly understood. | 2008 | 18937754 |
| the impact of laboratory characteristics on molecular detection of respiratory syncytial virus in a european multicentre quality control study. | the performance of nucleic acid amplification techniques for detecting respiratory syncytial virus (rsv) was evaluated in 25 laboratories across europe by an external quality assessment study. in addition, factors related to the diagnostic performance of laboratories were explored. the results of this quality control study show that the performance of laboratories for rsv diagnosis in europe is good, with an overall correct score of 88%. the type of assay (nested or real-time pcr vs. commercial ... | 2008 | 19046164 |
| immunoprophylaxis against respiratory syncytial virus (rsv) with palivizumab in children: a systematic review and economic evaluation. | to systematically review the effectiveness and cost-effectiveness of palivizumab for the prevention of respiratory syncytial virus (rsv) in children and examine prognostic factors to determine whether subgroups can be identified with important differences in cost-effectiveness. | 2008 | 19049692 |
| persistent adenovirus-mediated thymidine kinase gene expression in ovarian cancer cells increases cell killing efficacy over time. | adenovirus (adv)-mediated gene therapy with the thymidine kinase (tk) gene under control of the rous sarcoma virus (rsv) promotor followed by the administration of acyclovir has been established in vitro for the treatment of ovarian cancer cells and has been used as the basis for intraperitoneal phase i clinical trials. it is unclear how long a significant degree of transgene translation can be expected after adenovirus-mediated tk transduction, where the transcriptional complex is localized in ... | 2008 | 19035280 |
| antiviral activity of benzimidazole derivatives. i. antiviral activity of 1-substituted-2-[(benzotriazol-1/2-yl)methyl]benzimidazoles. | forty-three 2-[(benzotriazol-1/2-yl)methyl]benzimidazoles, bearing either linear (dialkylamino)alkyl- or bulkier (quinolizidin-1-yl)alkyl moieties at position 1, were evaluated in cell-based assays for cytotoxicity and antiviral activity against viruses representative of two of the three genera of the flaviviridae family, i.e. flaviviruses (yellow fever virus (yfv)) and pestiviruses (bovine viral diarrhoea virus (bvdv)), as hepaciviruses can hardly be used in routine cell-based assays. compounds ... | 2008 | 19035566 |
| molecular characterization of strains of respiratory syncytial virus identified in a hematopoietic stem cell transplant outpatient unit over 2 years: community or nosocomial infection? | respiratory syncytial virus (rsv) is recognized as the leading cause of nosocomial respiratory infection among hematopoietic stem cell transplant (hsct) recipients, causing considerable morbidity and mortality. rsv is easily transmitted by contact with contaminated surfaces, and in hsct units, more than 50% of rsv infections have been characterized as of nosocomial origin. from april 2001 to october 2002, rsv was identified by direct immunofluorescent assay in 42 symptomatic hsct recipients. sev ... | 2008 | 19041056 |
| overcoming t cell-mediated immunopathology to achieve safe rsv vaccination. | respiratory syncytial virus (rsv) is the leading cause of lower respiratory tract disease in young children. premature infants, immunocompromised individuals, and the elderly exhibit an increased risk for the development of severe disease after rsv infection. currently, there is not a safe and effective rsv vaccine available, in part due to our incomplete understanding of how severe immunopathology was induced following rsv infection of children previously immunized with a formalin-inactivated r ... | 2008 | 19057653 |
| the number of respiratory syncytial virus (rsv)-specific memory cd8 t cells in the lung is critical for their ability to inhibit rsv vaccine-enhanced pulmonary eosinophilia. | children that were administered a formalin-inactivated respiratory syncytial virus (fi-rsv) vaccine experienced enhanced respiratory disease, including pulmonary eosinophilia, after contracting a natural rsv infection. rsv vaccine-enhanced disease can be mimicked in balb/c mice immunized with either fi-rsv or with a recombinant vaccinia virus (vacv) expressing the rsv attachment (g) protein. we have recently demonstrated that memory cd8 t cells directed against the rsv immunodominant m2(82-90) e ... | 2008 | 19017987 |
| a predictive model for respiratory syncytial virus (rsv) hospitalisation of premature infants born at 33-35 weeks of gestational age, based on data from the spanish flip study. | the aim of this study, conducted in europe, was to develop a validated risk factor based model to predict rsv-related hospitalisation in premature infants born 33-35 weeks' gestational age (ga). | 2008 | 19063742 |
| antiviral activities of extracts from hong kong seaweeds. | we extracted six hong kong brown seaweed species with hot water for their antiviral properties. the cytotoxicity and antiviral activity of these extracts were tested by mtt [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenlytetrezolium bromide] method, cytopathic effect reduction assay, and plaque reduction assay. the antiviral effect was further determined by flow cytometric analysis. the results showed that most of these extracts inhibited the propagation of herpes simplex virus types 1 and 2 (hsv-1 an ... | 2008 | 19067465 |
| [action of an active compound rg2-1 from radix gentianae on antirespiratory syncytial virus in vitro]. | to observe an action of an active compound rg2-1 from radix gentianae on the antirespiratory syncytial virus(rsv) in vitro. | 2008 | 19069662 |
| ability of low-molecular-weight heparin to alleviate proteinuria by inhibiting respiratory syncytial virus infection. | low-molecular-weight heparin (lmwh) is a negatively charged glycoprotein and has a very similar structure to that of cell surface heparin sulfate (hs). thus, lmwh, an analog of hs, may inhibit positively charged respiratory syncytial virus (rsv) infection through cooperative electrostatic association. | 2008 | 19161362 |
| viral pathogens of acute lower respiratory tract infection in china. | to document the viral etiology of acute lower respiratory tract infection (alris) in chinese children. | 2008 | 19129564 |
| [viral respiratory tract infections in the first six months of life]. | respiratory syncytial virus and influenza virus infections are known causes of hospital admission in infants. it is less well known the pattern of virus infections in infants under 6 months of age in the outpatient setting. | 2008 | 19128739 |
| risk of respiratory syncytial virus in survivors with severe congenital diaphragmatic hernia. | during the follow-up period in surviving patients with severe congenital diaphragmatic hernia (cdh), respiratory complications, such as recurrence of cdh or chronic lung disease, have been reported to occur as a late complication. although some risk factors for deterioration of respiratory condition have been reported in cdh, the risk of respiratory syncytial virus (rsv) in postoperative cdh patients has not as yet been reported upon. | 2008 | 19143967 |
| enhancement of mucosal and cellular immune response in mice by vaccination with respiratory syncytial virus dna encapsulated with transfersome. | respiratory syncytial virus (rsv) is one of the principal causes of bronchiolitis and pneumonia in young children, and currently there is no safe and effective vaccine. dna vaccines encoding rsv surface glycoproteins are one option being examined. we evaluated the topical delivery of transfersome encapsulated dna vaccine for its ability to confer protection against rsv challenge in mice and to determine whether such delivery could induce strong and specific immunity against rsv. after topical va ... | 2008 | 19115938 |
| irregular and semi-regular polyhedral models for rous sarcoma virus cores. | whereas many viruses have capsids of uniquely defined sizes that observe icosahedral symmetry, retrovirus capsids are highly polymorphic. nevertheless, they may also be described as polyhedral foldings of a fullerene lattice on which the capsid protein (ca) is arrayed. lacking the high order of symmetry that facilitates the reconstruction of icosahedral capsids from cryo-electron micrographs, the three-dimensional structures of individual retrovirus capsids may be determined by cryo-electron tom ... | 2008 | 19122884 |
| [antibiotics prescription in infants hospitalized with respiratory syncytial virus pneumonia]. | although not useful, antibiotics are commonly prescribed in infants with viral pneumonia. | 2008 | 19107304 |
| [construction and preliminary panning of fab phage display antibody library against respiratory syncytial virus]. | to construct a human phage display antibody library, which will help to develop new drugs and vaccines against respiratory syncytial virus (rsv) and solve many of the issues that have limited the progression and application of murine monoclonal antibodies (mcabs) in the clinic. this can provide a platform for human antibody preparation and diagnosis, prophylaxis and therapy of rsv infection in children. | 2008 | 19102829 |
| [association of rantes gene promoter -28c/g polymorphism with respiratory syncytial virus bronchiolitis]. | respiratory syncytial virus (rsv) infects nearly all children under two years of age. it is poorly understood why a few children who were infected with rsv develop bronchiolitis that require hospital admission while most have a relatively minor illness. several recent studies have obtained some indications for the involvement of genetic heterogeneity in rsv bronchiolitis, implying that the clinical outcome of rsv infection perhaps is determined by genetic factors. regulated on activation, normal ... | 2008 | 19099677 |
| high seroprevalence of human metapneumovirus infection in children in chongqing, china. | the human metapneumovirus (hmpv) is a newly discovered respiratory viral pathogen that was first isolated in 2001 in the netherlands. its global distribution and long history of infection in humans have been well documented. in this study, we assessed the seropositivity of hmpv igg antibodies in children in chongqing, china. | 2008 | 19080178 |
| protective t cell immunity against respiratory syncytial virus is efficiently induced by recombinant bcg. | respiratory syncytial virus (rsv) is one of the leading causes of childhood hospitalization and a major health burden worldwide. unfortunately, because of an inefficient immunological memory, rsv infection provides limited immune protection against reinfection. furthermore, rsv can induce an inadequate th2-type immune response that causes severe respiratory tract inflammation and obstruction. it is thought that effective rsv clearance requires the induction of balanced th1-type immunity, involvi ... | 2008 | 19075247 |
| respiratory syncytial virus activity-- united states, july 2007-december 2008. | respiratory syncytial virus (rsv) is the most common cause of bronchiolitis and pneumonia in children aged <1 year and is a major cause of respiratory illness in older adults. rsv is transmitted person-to-person via close contact, droplets, and fomites. each year in the united states, an estimated 75,000-125,000 children aged <1 year are hospitalized with rsv. those at increased risk for hospitalization include premature infants meeting certain criteria and persons of any age with compromised re ... | 2008 | 19092759 |
| comprehensive detection of causative pathogens using real-time pcr to diagnose pediatric community-acquired pneumonia. | we have developed a real-time reverse transcription-pcr (rt-pcr) method to detect 13 respiratory viruses: influenza virus a and b; respiratory syncytial virus (rsv) subgroup a and b; parainfluenza virus (piv) 1, 2, and 3; adenovirus; rhinovirus (rv); enterovirus; coronavirus (oc43); human metapneumovirus (hmpv); and human bocavirus (hbov). the new method for detection of these viruses was applied simultaneously with real-time pcr for the detection of six bacterial pathogens in clinical samples f ... | 2008 | 19089556 |
| human bocavirus infection in hospitalized children in italy. | human bocavirus (hbov) was first discovered in sweden in 2005 and has now been found worldwide; however its role in clinically relevant diseases has not yet been clearly defined. | 2008 | 19453422 |
| human coronavirus nl63 infections in infants hospitalised with acute respiratory tract infections in south africa. | human coronavirus nl63 (hcov-nl63) is a novel respiratory virus which is associated with respiratory tract infections in children. | 2008 | 19453465 |
| role of influenza and other respiratory viruses in admissions of adults to canadian hospitals. | we sought to estimate age-specific hospitalization rates attributed to influenza and other virus for adults. | 2008 | 19453488 |
| effective delivery of igg-antibodies into infected cells via dendritic molecular transporter conjugate iggmt. | igg antibody-transporter conjugates enable intracellular uptake of biologically active igg antibodies that inhibit viral mediated syncytia formation in respiratory syncytial virus green fluorescent protein (rsv-gfp) infected human epithelial cells (hep-2). | 2008 | 19396384 |
| adenovirus-mediated thymidine kinase gene therapy and coxsackie adenovirus receptor expression in ovarian cancer cells. | coxsackie adenovirus receptor (car) expression is the main mechanism of adenovirus entry into target cells. it is unclear whether car expression itself is influenced by transduction with the adenovirus-rous sarcoma virus-thymidine kinase (adv-rsv-tk) gene therapy construct or by the subsequent intracellular accumulation of the tk gene product. antibody generation and characterization, immunocytochemistry, western blotting and 3-(4,5-dimethylthiazol)-2,5-diphenyl tetrazolium bromide (mtt) assay w ... | 2008 | 19287072 |
| cytomegalovirus infection and wheezing in infants. | bronchial asthma-like symptoms such as wheezing are commonly associated with respiratory tract infection including respiratory syncytial virus (rsv) infection in infants. no study on the association of wheezing with cytomegalovirus (cmv) infection in infancy has been reported, although cmv infection has been observed to play some role in prolonged and intractable wheezing in limited cases. | 2008 | 19261114 |
| etiology of viral pneumopathies in patients in intensive care unit under mechanical ventilation. | the objective of this work was to define the etiology of viral pneumopathies at the patients from reanimation section being under mechanical ventilation, making reference to viruses with respiratory tropism, and also to chlamydia pneumoniae and mycoplasma pneumoniae. the subjects were 36 patients hospitalized into service of medical reanimation from chu caen and who needed mechanical ventilation more than 48 hours. the samples from the patients were mostly nasal aspirate, 1 bronchial aspirate an ... | 2008 | 19284161 |
| [respiratory syncytial virus: as an etiological agent of respiratory tract infection in children and adults]. | respiratory syncytial virus is the most important cause of respiratory tract infection in infants, young children and immunocompromised adults. rsv disease spectrum includes a wide array of respiratory symptoms, from rhinitis and otits media to pneumonia and bronchiolitis. studies have implicated severe rsv infection early in life as a risk factor for subsequent development of reactive airway disease. rsv-infected patients indicated increased levels of th2 cytokines and ige in the patients sera, ... | 2008 | 19209739 |
| epidemiology of respiratory syncytial virus in various regions within north carolina during multiple seasons. | the centers for disease control and prevention (cdc) monitors the occurrence of respiratory syncytial virus (rsv) in the united states and has historically reported on activity at the regional level. prior to the 2007-2008 rsvseason, the cdc did not report seasonal rsv data for cities within north carolina or for the state. the purpose of the present study is to characterize rsv seasonal activity within north carolina and to determine the appropriate months in which at-risk children should recei ... | 2008 | 19256181 |
| [construction and identification of replication deficient recombinant adenovirus encoding f gene of subgroup a human respiratory syncytial virus]. | a strain of replication deficient recombinant adenvirus encoding fusion glycoprotein (f) of subgroup a human respiratory syncytial virus (rsv) was constructed and the expression of f was identified. | 2008 | 19544635 |
| [in vitro inhibitory mechanism of respiratory syncytial virus with solution prescription of zhidanhuayu]. | to study the effective part of solution prescription of zhidanhuayu (zdhy) against respiratory syncytial virus (rsv) in vitro. | 2008 | 19544654 |
| prevalence of bordetella pertussis and bordetella parapertussis in samples submitted for rsv screening. | the clinical presentation of bordetella pertussis can overlap with that of respiratory syncytial virus (rsv); however, management differs. | 2008 | 19561728 |
| a new assay system for evaluation of developmental immunotoxicity of chemical compounds using respiratory syncytial virus infection to offspring mice. | we evaluated the effect of 6-propyl-2-thiouracil (ptu), an anti-thyroid agent, on developmental immunity using respiratory syncytial virus (rsv) infection to offspring mice as a new risk assessment for brominated flame retardants (bfrs), because some bfrs are suspected of affecting the thyroid system. pregnant mice were exposed to ptu in drinking water from gestation day 10 to weaning on postnatal day 21. their offspring mice were infected intranasally with rsv. exposure of 100ppm ptu significan ... | 2008 | 21783838 |
| effects of decabrominated diphenyl ether (dbde) on developmental immunotoxicity in offspring mice. | decabrominated diphenyl ether (dbde), a representative brominated flame retardant ubiquitous in the environment, is suspected of being hazardous to humans. we evaluated the developmental immunotoxicity of dbde by an assay system using respiratory syncytial virus (rsv) infection in offspring mice. pregnant mice were continuously exposed to dbde (10, 100, 1000, or 10,000ppm) in the diet from gestation day 10 to weaning on postnatal day 21. offspring mice born to these dams were intranasally infect ... | 2008 | 21791381 |
| management of upper respiratory tract infections in children. | upper respiratory tract infection (urti) occurs commonly in both children and adults and is a major cause of mild morbidity. it has a high cost to society, being responsible for absenteeism from school and work and unnecessary medical care, and is occasionally associated with serious sequelae. urtis are usually caused by several families of virus; these are the rhinovirus, coronavirus, parainfluenza, respiratory syncytial virus (rsv), adenovirus, human metapneumovirus, influenza, enterovirus and ... | 2008 | 21603094 |
| immunotherapy of hsv infections – antibody delivery | passive immunization involves utilizing polyclonal or monoclonal antibodies as a form of immunotherapy. antibodies can mediate their effects through several mechanisms, including opsonization and c-mediated lysis, but in particular antibody-dependent cell-mediated cytolysis (adcc) and neutralization. antibody immunotherapy has been demonstrated to be efficacious for the treatment and prevention of infection or disease caused by viruses other than herpes simplex virus (hsv ... | 2007 | 21348081 |
| a patient-centric, internet-based application for the data management of a multi-centre respiratory syncytial virus prophylaxis program. | the success of respiratory syncytial virus (rsv) prophylaxis programs is partially dependent upon meticulous tracking of at risk infants, and excellent data management. we designed and built an integrated, shared, user-driven but infant-centric data management application that enables an infants healthcare provider to determine an infants rsv prophylaxis status throughout the rsv season. the security and confidentiality of each infants information is protected by rigorous integrity constraints t ... | 2007 | 18693968 |
| respiratory syncytial virus: an important cause of acute respiratory illness among young adults undergoing military training. | military recruits receiving training are vulnerable to acute respiratory disease and a significant proportion of illness is due to unidentified pathogens. while some countries use surveillance programs to monitor such illness, few data exist for recruits of the british armed forces. | 2007 | 18846262 |
| first detection of human metapneumovirus in children with respiratory infections in romania. | the human metapneumovirus (hmpv) was first isolated in 2001 in the netherlands (van der hoogen and collaborators) from a nasopharyngeal aspirate sampled from an infant. based on the morphological, biochemical and genetic characteristics, the hmpv was initially classified in the genus metapneumovirus with the avian metapneumovirus (apv), the agent causing the respiratory infections of the upper tract in turkeys and other birds. subsequently, together with the respiratory syncytial virus (rsv), it ... | 2007 | 18928061 |
| palivizumab: a review of its use in the protection of high risk infants against respiratory syncytial virus (rsv). | respiratory syncytial virus (rsv) is a leading cause of hospitalization in children less than 1 year of age and causes substantial morbidity. although there is not currently a vaccine available to prevent rsv infection, prophylaxis with the humanized monoclonal antibody palivizumab has been shown to reduce the rate of rsv hospitalization in premature infants and those infants with chronic lung disease or congenital heart disease. because palivizumab has not been shown to have a beneficial clinic ... | 2007 | 19707346 |
| no evidence for an association between infections with wu and ki polyomaviruses and respiratory disease. | wu virus (wuv) and ki polyomavirus (kipyv) are newly discovered related human polyomaviruses detected in respiratory samples. to investigate their potential role in respiratory disease, we determined their frequencies of detection, clinical presentations and epidemiological characteristics among samples referred for diagnostic respiratory virus testing. | 2007 | 17997354 |
| notch ligand delta-like 4 regulates disease pathogenesis during respiratory viral infections by modulating th2 cytokines. | recent data have indicated that an important instructive class of signals regulating the immune response is notch ligand-mediated activation. using quantitative polymerase chain reaction, we observed that only delta-like 4 (dll4) was up-regulated on bone marrow-derived dendritic cells after respiratory syncytial virus (rsv) infection, and that it was dependent on myd88-mediated pathways. using a polyclonal antibody specific for dll4, the development of rsv-induced disease was examined. animals t ... | 2007 | 17998388 |
| respiratory virus-induced regulation of asthma-like responses in mice depends upon cd8 t cells and interferon-gamma production. | respiratory virus infections can significantly influence the development of airway disease by both predisposing and exacerbating the developing lung immune environment. in contrast, the initiation of a more desirable anti-viral response may better prepare the local environment and protect it from developing an adverse long-term disease phenotype. balb/c or c57bl/6 mice exposed to respiratory syncytial virus (rsv) infection at the same time as allergen sensitization were assessed for airway funct ... | 2007 | 17991711 |
| immunization of macaques with formalin-inactivated human metapneumovirus induces hypersensitivity to hmpv infection. | human metapneumovirus (hmpv), a member of the family paramyxoviridae, is an important cause of acute respiratory tract disease. in the 1960s, vaccination with formalin-inactivated paramyxovirus preparations--respiratory syncytial virus (rsv) and measles virus (mv)--resulted in predisposition for enhanced disease upon natural infection. we have produced a formalin-inactivated hmpv preparation (fi-hmpv), which was used to immunize young cynomolgus macaques. six days after challenge fi-hmpv-primed ... | 2007 | 17996988 |
| lung function in prematurely born infants after viral lower respiratory tract infections. | chronic respiratory morbidity has been reported in prematurely born infants after respiratory syncytial virus (rsv) and human metapneumovirus (hmpv) lower respiratory tract infections (lrtis). the aim of this study was to determine the impact of viral lrti on lung function at follow-up of prematurely born infants. | 2007 | 17984809 |
| motavizumab, a neutralizing anti-respiratory syncytial virus (rsv) monoclonal antibody significantly modifies the local and systemic cytokine responses induced by rsv in the mouse model. | motavizumab (medi-524) is a monoclonal antibody with enhanced neutralizing activity against rsv. in mice, motavizumab suppressed rsv replication which resulted in significant reduction of clinical parameters of disease severity. we evaluated the effect of motavizumab on the local and systemic immune response induced by rsv in the mouse model. balb/c mice were intranasally inoculated with 106.5 pfu rsv a2 or medium. motavizumab was given once intraperitoneally (1.25 mg/mouse) as prophylaxis, 24 h ... | 2007 | 17961258 |
| pharmacy management of vaccines. | although standard vaccines have traditionally been granted full coverage in managed care, the recent introduction of several novel vaccine products has necessitated the revision of pharmacy management strategies throughout the nation. | 2007 | 17955623 |
| [influenza in poland in 2005]. | a total number of 773,234 cases of influenza and influenza-like illness registered in poland in 2004 (incidence 1921.4 per 100,000 population). regionally the incidence ranged from 3391.8 per 100,000 population in mazowieckie to 364.0 in swietokrzyskie. children and adolescents under 15 years of age accounted for 34.5% of all cases (age specific incidence 4045.3 per 100,000). in this age group the incidence varied regionally from 6381.1 in mazowieckie to 797.6 in swietokrzyskie. 1469 patients (0 ... | 2007 | 17956033 |
| [signal transduction in respiratory syncytial virus infection-induced thymic stromal lymphopoietin expression in human epithelial cells]. | to explore the mechanism of signal transduction in respiratory syncytial virus (rsv)-induced expression of thymic stromal lymphopoietin (tslp) in bronchial epithelial cells. | 2007 | 17959543 |
| venezuelan equine encephalitis virus replicon particles encoding respiratory syncytial virus surface glycoproteins induce protective mucosal responses in mice and cotton rats. | respiratory syncytial virus (rsv) is an important viral pathogen that causes severe lower respiratory tract infection in infants, the elderly, and immunocompromised individuals. there are no licensed rsv vaccines to date. to prevent rsv infection, immune responses in both the upper and lower respiratory tracts are required. previously, immunization with venezuelan equine encephalitis virus replicon particles (vrps) demonstrated effectiveness in inducing mucosal protection against various pathoge ... | 2007 | 17928349 |
| immunological characterization of respiratory syncytial virus n protein epitopes recognized by human cytotoxic t lymphocytes. | virus-specific cytotoxic t lymphocytes (ctls) are crucial for the control of respiratory syncytial virus (rsv) infection. this study has identified ctl epitopes of the rsv n protein in healthy subjects. we screened the primary structure of the n protein for hla-a 0201-binding amino acid consensus motifs, identifying three peptides designated as n-rsv1, n-rsv2, and n-rsv3. these peptides were used to generate ctl lines by stimulating human hla-a 02.01 peripheral blood mononuclear cells (pbmcs) in ... | 2007 | 17931110 |
| comparison of strain-specific antibody responses during primary and secondary infections with respiratory syncytial virus. | respiratory syncytial virus (rsv) is a major cause of lower respiratory tract infection in infants. rsv repeatedly reinfects individuals: this may be due in part to the variability of the attachment (g) glycoprotein and changes in this protein have been shown to be under positive selection. infants experiencing their primary infection show a genotype-specific antibody response with respect to the variable regions of the g protein. a prospective study of rsv infections in a birth cohort in rural ... | 2007 | 17935184 |
| respiratory syncytial virus infections in hospitalized infants: association between viral load, virus subgroup, and disease severity. | the relationships between host factors, virus strain, viral load, and illness severity in respiratory syncytial virus (rsv)-induced bronchiolitis are poorly defined. these relationships were evaluated prospectively in 81 previously healthy infants hospitalized with rsv bronchiolitis. disease severity was determined by the respiratory rate, the duration of hospitalization, and whether patients during their hospitalization required pediatric intensive care unit admission or mechanical ventilation. ... | 2007 | 17935185 |
| [indications and prescription modalities of palivizumab in neonates]. | palivizumab is a recombinant humanized monoclonal antibody against the f glycoprotein of respiratory syncytial virus (rsv). it has been licensed since 1999 in france for the prevention of serious lower respiratory-tract infection caused by rsv requiring hospitalization in children born at 35 weeks gestation or less and who are less than 6 months old at the onset of rsv season, or in children less than 2 years old who have received treatment for bronchopulmonary dysplasia within the last 6 months ... | 2007 | 17939952 |
| respiratory syncytial virus infection in 406 hospitalized premature infants: results from a prospective german multicentre database. | premature birth, chronic lung disease of prematurity (cld), congenital heart disease and immunodeficiency predispose to a higher morbidity and mortality in respiratory syncytial virus (rsv) infection. this study describes the preterms hospitalised with rsv infection from the prospective german dsm rsv paed database. the dms rsv paed database was designed for the prospective multicentre documentation and analysis of clinically relevant aspects of the management of inpatients with rsv infection. t ... | 2007 | 17943313 |
| respiratory viral infections in transplant recipients. | a wide range of viruses affect the respiratory tract of transplant recipients, including adenovirus, influenza, human metapneumovirus, parainfluenza virus, respiratory syncytial virus (rsv) and rhinovirus. prospective studies using contemporary diagnostic techniques have recently improved our understanding of the epidemiology and importance of these respiratory viruses among transplant recipients. from these studies, rhinovirus, in particular, has been shown to be one of the most common causes o ... | 2007 | 17944270 |
| respiratory syncytial virus infection in adults. | originally considered as only a paediatric pathogen, respiratory syncytial virus (rsv) has recently been shown to be a significant cause of respiratory illness among elderly and high-risk adults. approximately 170,000 hospitalizations and 10,000 deaths associated with rsv occur annually in people over the age of 65 years in the united states. although rhinorrhoea and wheezing are common symptoms among adults, the clinical syndrome associated with rsv is not distinctive and thus laboratory method ... | 2007 | 17944273 |
| vii international symposium on respiratory viral infections. | the vii international symposium on respiratory viral infections was a multidisciplinary forum for the presentation of recent advances in respiratory virus research with special emphasis on antiviral therapies and vaccine strategies. topics covered in invited lectures included detection of novel respiratory viral pathogens and viral evolution, characterization of the 1918 pandemic virus, human metapneumovirus infections, human respiratory epithelial cultures for studying viral pathogenesis, the r ... | 2007 | 17944274 |
| [construction and preparation of helper-dependent adenoviral vector expressing human respiratory syncytial virus f gene]. | to construct a helper-dependent adenoviral vector expressing human respiratory syncytial virus (rsv) subgroup a f gene, and finish large scale preparation, purification and identification of the vector. f gene under the control of cmv promoter was subcloned into a shutle vector psc11, and then cloned into hadd plasmid psc15b. the hdad/f genome was liberated by removing the bacterial sequences from the resulting plasmid psc15b/f digested with restriction enzyme pme i, and then the linear hdad/f d ... | 2007 | 17944372 |
| recombinant sendai virus induces t cell immunity against respiratory syncytial virus that is protective in the absence of antibodies. | respiratory syncytial virus (rsv) causes severe respiratory disease in infants and a vaccine is highly desirable. the fusion (f) protein of rsv is an important vaccine target, but the contribution of f-specific t cells to successful vaccination remains unclear. we studied the immune response to vaccination of mice with a recombinant sendai virus expressing rsv f (rsev f). rsev f induced protective neutralizing antibody and rsv f-specific ctl responses. t cell immunity was stronger than that indu ... | 2007 | 17904538 |
| cd8 t cells inhibit respiratory syncytial virus (rsv) vaccine-enhanced disease. | vaccination of children with a formalin-inactivated (fi) respiratory syncytial virus (rsv) vaccine led to exacerbated disease including pulmonary eosinophilia following a natural rsv infection. immunization of balb/c mice with fi-rsv or a recombinant vaccinia virus (vv) expressing the rsv attachment (g) protein (vvg) results in a pulmonary th2 response and eosinophilia after rsv challenge that closely mimics the rsv vaccine-enhanced disease observed in humans. the underlying causes of rsv vaccin ... | 2007 | 17911628 |
| inhibition of alpharetrovirus replication by a range of human apobec3 proteins. | the mammalian apobec3 family of cytidine deaminases includes members that can act as potent inhibitors of retroviral infectivity and retrotransposon mobility. here, we have examined whether the alpharetrovirus rous sarcoma virus (rsv) is susceptible to inhibition by a range of human apobec3 proteins. we report that rsv is highly susceptible to inhibition by human apobec3g, apobec3f, and apobec3b and moderately susceptible to inhibition by human apobec3c and apobec3a. for all five proteins, inhib ... | 2007 | 17913830 |
| understanding respiratory syncytial virus (rsv) vaccine-enhanced disease. | respiratory syncytial virus (rsv) is the most common cause of lower respiratory tract infection in infants and children worldwide. in addition, rsv causes serious disease in elderly and immune compromised individuals. rsv infection of children previously immunized with a formalin-inactivated (fi)-rsv vaccine is associated with enhanced disease and pulmonary eosinophilia that is believed to be due to an exaggerated memory th2 response. as a consequence, there is currently no licensed rsv vaccine ... | 2007 | 17917067 |
| regulation of airway eosinophil and neutrophil infiltration by alpha-galactosylceramide in a mouse model for respiratory syncytial virus (rsv) vaccine-augmented disease. | respiratory syncytial virus (rsv) is a leading cause of respiratory disease among infants, the elderly and immunocompromised adults. in this study, we assessed the effects of alpha-galactosylceramide, a known immunoregulatory lipid, on liposomal rsv vaccine-induced responses in balb/c mice subsequently challenged with rsv. liposomes containing a recombinant fragment of the rsv g protein were prepared with and without alpha-galactosylceramide and used to immunize mice by the intranasal route. the ... | 2007 | 17920164 |
| induction of nkg2d ligands on human dendritic cells by tlr ligand stimulation and rna virus infection. | monocyte-derived dendritic cells (mdcs) and nk cells are reciprocally activate via cytokines and cell-cell contact. although seven human nkg2d ligands (nkg2dls), ul16-binding proteins (ulbp) 1, 2, 3 and 4, retinoic acid early transcript 1g (raet1g) and mhc class i-related chains a and b, have been reported, the differential distribution and roles of these ligands in the maturation of human mdcs have not been elucidated. in the present study, we produced polyclonal antibodies (pabs) directed agai ... | 2007 | 17878262 |
| lung function and bronchial hyper-responsiveness 11 years after hospitalization for bronchiolitis. | atopic infants hospitalized for wheezing not caused by respiratory syncytial virus (rsv) carry the highest risk for later asthma. in the present paper, early risk factors for later lung function abnormalities and for bronchial hyper-responsiveness (bhr) were evaluated in 81 children, hospitalized for bronchiolitis in infancy, at the median age of 12.3 years. | 2007 | 17880415 |
| transforming growth factor beta enhances respiratory syncytial virus replication and tumor necrosis factor alpha induction in human epithelial cells. | asthma is characterized as a chronic inflammatory disease associated with significant tissue remodeling. patients with asthma are more susceptible to virus-induced exacerbation, which subsequently can lead to increased rates of hospitalization and mortality. while the most common cause of asthma-related deaths is respiratory viral infections, the underlying factors in the lung environment which render asthmatic subjects more susceptible to viral exacerbation are not yet identified. since transfo ... | 2007 | 17202225 |
| respiratory syncytial virus infection and recurrent wheeze/asthma in children under five years: an epidemiological survey. | to determine the rates of respiratory syncytial virus (rsv) infection in children under 5 years of age with recurrent wheeze or asthma and compare their clinical presentation, course and outcome with asthmatic children of the same age who did not have rsv. | 2007 | 17207052 |
| preliminary evaluation of a multiplex reverse transcription-pcr assay combined with a new dna chip hybridization assay for detecting respiratory syncytial virus. | dna chips represent a major advance in microbiology laboratories, enabling the detection of a wide range of possible pathogens using a single test. this study compared a multiplex reverse transcription-pcr combined with dna chip hybridization (prodect bcs rv chip; bcs biotech) with the indirect immunofluorescence test commonly used to detect respiratory viruses. a total of 39 respiratory viruses (38 respiratory syncytial viruses [rsvs] and 1 influenza a virus) were detected in samples from 96 pa ... | 2007 | 17881543 |
| identification of nyasol and structurally related compounds as the active principles from anemarrhena asphodeloides against respiratory syncytial virus (rsv). | three known phenolic compounds, (-)-(r)-nyasol (= 4,4'-(1z,3r)-penta-1,4-diene-1,3-diyldiphenol; 1), its derivative 2, and broussonin a (3)--isolated from the rhizomes of anemarrhena asphodeloides--were for the first time identified as the active principles capable of efficient respiratory-syncytial-virus (rsv) inhibition. the ic50 values of 1-3 against the rsv-a2 strain, propagated in hep-2 cells, were determined, their activities being higher than that of the standard antiviral drug ribavirin ... | 2007 | 17886842 |
| maternal alcohol ingestion reduces surfactant protein a expression by preterm fetal lung epithelia. | in addition to neurodevelopmental effects, alcohol consumption at high levels during pregnancy is associated with immunomodulation and premature birth. premature birth, in turn, is associated with increased susceptibility to various infectious agents such as respiratory syncytial virus (rsv). the initial line of pulmonary innate defense includes the mucociliary apparatus, which expels microorganisms trapped within the airway secretions. surfactant proteins a and d (sp-a and sp-d, respectively) a ... | 2007 | 17889311 |
| virucidal activities of medium- and long-chain fatty alcohols and lipids against respiratory syncytial virus and parainfluenza virus type 2: comparison at different ph levels. | recent studies have shown that some lipids and fatty alcohols have microbicidal activities against a broad variety of pathogens. in this study, virucidal activities of fatty acids, monoglycerides and fatty alcohols were tested against respiratory syncytial virus (rsv) and human parainfluenza virus type 2 (hpiv2) at different concentrations, times and ph levels. the most active compounds were mixed with milk products and fruit juices and the mixtures tested for virucidal effects. the aim was to d ... | 2007 | 17891329 |
| differential response of human naive and memory/effector t cells to dendritic cells infected by respiratory syncytial virus. | in vitro studies have contributed substantially to the understanding of immunopathology of respiratory syncytial virus (rsv)-mediated disease. in the present study we compared the effect of rsv-infected dendritic cells on the time-course of the primary and memory/effector t cell response in vitro. cultures with uninfected dendritic cells known to elicit t helper 2 (th2) responses and with polyinosinic-polycytidylic acid (poly-ic)-stimulated dendritic cells known to elicit th1 responses served as ... | 2007 | 17892510 |
| do inhaled corticosteroids improve oxygen saturation in infants with respiratory syncytial virus (rsv) bronchiolitis? | 2007 | 17896613 | |
| systemic aav-9 transduction in mice is influenced by animal age but not by the route of administration. | adeno-associated virus (aav) serotype-9 (aav-9) has attracted great attention as an optimal vehicle for body-wide gene delivery. here we examined the effect of animal age (newborn vs adult) and the route of administration (intravenous vs intra-arterial) on systemic aav-9 transduction. we delivered an alkaline phosphatase (ap) reporter gene aav vector (av.rsv.ap) to either newborn (via either the facial vein or the left ventricular cavity) or adult (via tail vein) c57bl/10 mice. at 12 weeks' post ... | 2007 | 17898796 |
| role of rhinovirus in hospitalized infants with respiratory tract infections in spain. | rhinovirus is a recognized cause of common cold, proven to cause asthma exacerbations in children. in spain, no description exists, as yet, as to the degree of burden rhinovirus infections represent among hospitalized infants. our aim was to describe rhinovirus infections in hospitalized children, under 2 years of age, and to compare these with patients infected with respiratory syncytial virus (rsv). | 2007 | 17901795 |