Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| batch medication of intestinal infections in nursery pigs-a randomised clinical trial on the efficacy of treatment strategy, type of antibiotic and bacterial load on average daily weight gain. | previous research projects have demonstrated the need for better diagnostic tools to support decisions on medication strategies for infections caused by escherichia coli f4 (f4) and f18 (f18), lawsonia intracellularis (li) and brachyspira pilosicoli (pilo). this study was carried out as a randomised clinical trial in three danish pig herds and included 1047 nursery pigs, distributed over 10 batches and 78 pens. the objectives of this study were: (1) to assess the effect of four 5-day treatment s ... | 2017 | 28107883 |
| the first report of brucella suis biovar 1 isolation in human in turkey. | brucella melitensis and b. abortus are the species generally isolated from human samples in turkey. several studies have also demonstrated the presence of antibodies against b. canis. | 2017 | 26947547 |
| a pcr-rflp assay to detect and type cytolethal distending toxin (cdt) genes in campylobacter hyointestinalis. | campylobacter hyointestinalis is considered as an emerging zoonotic pathogen. we have recently identified two types of cytolethal distending toxin (cdt) gene in c. hyointestinalis and designated them as chcdt-i and chcdt-ii. in this study, we developed a pcr-restriction fragment length polymorphism (rflp) assay that can differentiate chcdt-i from chcdt-ii. when the pcr-rflp assay was applied to 17 other campylobacter strains and 25 non-campylobacter strains, pcr products were not obtained irresp ... | 2017 | 27916784 |
| revisiting the taxonomy of the family circoviridae: establishment of the genus cyclovirus and removal of the genus gyrovirus. | the family circoviridae contains viruses with covalently closed, circular, single-stranded dna (ssdna) genomes, including the smallest known autonomously replicating, capsid-encoding animal pathogens. members of this family are known to cause fatal diseases in birds and pigs and have been historically classified in one of two genera: circovirus, which contains avian and porcine pathogens, and gyrovirus, which includes a single species (chicken anemia virus). however, over the course of the past ... | 2017 | 28155197 |
| involvement of multiple chlamydia suis genotypes in porcine conjunctivitis. | chlamydia suis has been detected in numerous disease conditions of pigs, particularly in eye infections. this study examined recurring conjunctivitis cases in five commercial pig farms in japan. 40.5% of the cases were identified as chlamydia positive using impression cytology of ocular smears and a genus-specific direct fluorescent antibody. c. suis was detected in 59.5% of the samples using pcr tests targeting 16s-23s rrna intergenic spacer region (isr) and ompa gene. genetic analysis of pcr a ... | 2017 | 28345224 |
| the chlamydia suis genome exhibits high levels of diversity, plasticity, and mobile antibiotic resistance: comparative genomics of a recent livestock cohort shows influence of treatment regimes. | chlamydia suis is an endemic pig pathogen, belonging to a fascinating genus of obligate intracellular pathogens. of particular interest, this is the only chlamydial species to have naturally acquired genes encoding for tetracycline resistance. to date, the distribution and mobility of the tet-island are not well understood. our study focused on whole genome sequencing of 29 c. suis isolates from a recent porcine cohort within switzerland, combined with data from usa tetracycline-resistant isolat ... | 2017 | 28338777 |
| chlamydia suis and chlamydia trachomatis induce multifunctional cd4 t cells in pigs. | chlamydia trachomatis infections are the most prominent bacterial sexually-transmitted disease world-wide and a lot of effort is put into the development of an effective vaccine. pigs have been shown to be a valuable animal model for c. trachomatis vaccine development. the aim of this study was to decipher the t-cell-mediated immune response to chlamydial infections including c. trachomatis and c. suis, the chlamydia species naturally infecting pigs with a demonstrated zoonotic potential. vagina ... | 2017 | 27894718 |
| identification and genetic characterization of classical swine fever virus isolates in brazil: a new subgenotype. | the classical swine fever (csf) is a highly contagious viral disease of pigs and wild boar. the csf causes great economic losses for pork production and the occurrence of the disease is notifiable to the oie. the objective of this work was to identify and characterize csf virus isolates from brazil. seven viral isolates were obtained and the full-length e2 sequences were analyzed. phylogenetic analysis revealed a different segregation pattern between brazilian isolates and members of subgenotype ... | 2017 | 27815697 |
| complete genome sequence of classical swine fever virus isolated near the demilitarized zone in the republic of korea. | the yc16cs (genotype 2.1) strain of classical swine fever virus, isolated from infected pigs in yeoncheon province, republic of korea, near the demilitarized zone, has a high identity with the pc11wb strain of the virus. this is significant in that it is the first case of transmission from wild boars to breed pigs revealed by an epidemiological investigation. | 2017 | 28385854 |
| the core protein of a pestivirus protects the incoming virus against ifn-induced effectors. | a multitude of viral factors - either inhibiting the induction of the ifn-system or its effectors - have been described to date. however, little is known about the role of structural components of the incoming virus particle in protecting against ifn-induced antiviral factors during or immediately after entry. in this study, we take advantage of the previously reported property of classical swine fever virus (family flaviviridae, genus pestivirus) to tolerate a deletion of the core protein if a ... | 2017 | 28290554 |
| visual detection and differentiation of classic swine fever virus strains using nucleic acid sequence-based amplification (nasba) and g-quadruplex dnazyme assay. | the split g-quadruplex dnazyme has emerged as a valuable tool for visual dna detection. here, we successfully integrated colorimetric split g-quadruplex dnazyme assay with nucleic acid sequence-based amplification to generate a novel detection approach, allowing visual and rapid detection for the rna of shimen and hclv strains of classic swine fever virus (csfv). csfv is a rna virus that causes a highly contagious disease in domestic pigs and wild boar. with this method, we were able to detect a ... | 2017 | 28287135 |
| efficacy evaluation of the c-strain-based vaccines against the subgenotype 2.1d classical swine fever virus emerging in china. | classical swine fever (csf) is a devastating infectious disease of pigs caused by classical swine fever virus (csfv). the disease has been controlled following extensive vaccination with the lapinized attenuated vaccine c-strain for decades in china. however, frequent csf outbreaks occurred recently in a large number of c-strain-vaccinated pig farms in china and a new subgenotype 2.1d of csfv has been reported to be responsible for the outbreaks. here we analyzed the molecular variations and ant ... | 2017 | 28284603 |
| novel poly-uridine insertion in the 3'utr and e2 amino acid substitutions in a low virulent classical swine fever virus. | in this study, we compared the virulence in weaner pigs of the pinar del rio isolate and the virulent margarita strain. the latter caused the cuban classical swine fever (csf) outbreak of 1993. our results showed that the pinar del rio virus isolated during an endemic phase is clearly of low virulence. we analysed the complete nucleotide sequence of the pinar del rio virus isolated after persistence in newborn piglets, as well as the genome sequence of the inoculum. the consensus genome sequence ... | 2017 | 28284595 |
| molecular characterization of classical swine fever virus isolates from india during 2012-14. | classical swine fever is a highly contagious and economically important viral disease of pigs. outbreaks of classical swine fever virus (csfv) were recorded in different places in the kamrup district of assam in india between the years 2012 and 2014. the nucleotide sequences of the 10 csfv isolates were analyzed based on the partial nucleotide sequences of the e2, 5'ntr and ns5b genes. phylogenetic analysis indicated the dominance of subgroup 2.2 along with 2.1 strains in the northeast part of i ... | 2017 | 28279702 |
| detection of classical swine fever virus infection by individual oral fluid of pigs following experimental inoculation. | we evaluated the use of oral fluid as an alternative to serum samples for classical swine fever virus (csfv) detection. individual oral fluid and serum samples were collected at different times post-infection from pigs that were experimentally inoculated with csfv alfort 187 strain. we found no evidence of csfv neutralizing antibodies in swine oral fluid samples under our experimental conditions. in contrast, real-time reverse transcription-polymerase chain reaction could detect csfv nucleic aci ... | 2017 | 28114861 |
| intrauterine inoculation of minipigs with chlamydia trachomatis during diestrus establishes a longer lasting infection compared to vaginal inoculation during estrus. | advanced animal models, such as minipigs, are needed for the development of a globally requested human chlamydia vaccine. previous studies have shown that vaginal inoculation of sexually mature göttingen minipigs with chlamydia trachomatis resulted in an infection lasting only 3-5 days. the aim of this study was to evaluate the effect of targeting the upper porcine genital tract by transcervical and transabdominal intrauterine inoculation, compared to previously performed vaginal inoculation. fu ... | 2017 | 28189786 |
| competitive replication kinetics and pathogenicity in pigs co-infected with historical and newly invading classical swine fever viruses. | classical swine fever (csf), an economically important and highly contagious disease of pigs, is caused by classical swine fever virus (csfv). in taiwan, csfvs from field outbreaks belong to two distinct genotypes. the historical genotype 3.4 dominated from the 1920s to 1996, and since 1996, the newly invading genotype 2.1 has dominated. to explain the phenomenon of this virus shift in the field, representative viruses belonging to genotypes 2.1 and 3.4 were either inoculated alone (single infec ... | 2017 | 27889614 |
| quasispecies composition and diversity do not reveal any predictors for chronic classical swine fever virus infection. | classical swine fever (csf) can run acute, chronic, and prenatal courses in both domestic pigs and wild boar. although chronic infections are rare events, their epidemiological impact is very high due to the long-term shedding of virus. so far, little is known about the factors that influence disease course and outcome from either the host or virus's perspective. to elucidate the viral determinants, we analyzed the role of the viral populations for the development of chronic csf virus (csfv) inf ... | 2017 | 27885563 |
| post-natal persistent infection with classical swine fever virus in wild boar: a strategy for viral maintenance? | in this study, fifteen wild boar piglets were intranasally inoculated <10 h after birth with the moderately virulent classical swine fever virus (csfv) strain catalonia 01. at 5 days post-inoculation, seven other animals within 48 h of birth were put in contact with them. viral replication and innate and specific immune responses were evaluated. of the inoculated animals, 46.67% remained post-natally persistently infected and were apparently healthy with neither humoral nor cellular immunologica ... | 2017 | 26234886 |
| presence of clostridium difficile in pig faecal samples and wild animal species associated with pig farms. | to determine the presence of clostridium difficile on fattening pig farms in north-eastern spain. | 2017 | 27990723 |
| survival and prevalence of clostridium difficile in manure compost derived from pigs. | pigs, particularly piglets, have been identified as reservoir hosts of clostridium difficile. to examine the survival ability of this pathogen in pig feces-based manure compost, c. difficile spores, which were prepared to contain as few vegetative cells as possible, were artificially inoculated into pig feces and incubated at different temperatures. while c. difficile survived in the feces incubated at temperatures below 37 °c for over 30 days, cell numbers gradually decreased at thermophilic te ... | 2017 | 27871997 |
| prevalence and molecular characterization of clostridium difficile isolates from a pig slaughterhouse, pork, and humans in taiwan. | clostridium difficile causes antibiotic-associated diarrhea in both humans and animals. the ribotype 078, predominant in food animals, is associated with community-acquired c. difficile infection, and c. difficile is suggested to be a foodborne pathogen. recently, the c. difficile ribotype 078 lineage emerged in patients and pigs in taiwan. this study aimed to investigate the prevalence and molecular characterization of c. difficile isolated from a pig slaughterhouse, retail meat, ready-to-eat m ... | 2017 | 27870984 |
| identification, isolation, and phylogenetic analysis of clostridium perfringens type a and type c from wild boar ( sus scrofa ) in the people's republic of china. | clostridium perfringens is a gram-positive, anaerobic, spore-forming bacterium that can induces gas gangrene or enteritis in poultry and humans and many other mammalian species. here, we report an outbreak of c. perfringens type a and type c coinfection in wild boars ( sus scrofa ). in february 2016, 10 dead wild boars, including two fresh carcasses, were found in zhaosu county, xinjiang province, people's republic of china. the two fresh carcasses were included in this study. two strains of c. ... | 2017 | 28328352 |
| detection of zaire ebolavirus in swine: assay development and optimization. | ebolaviruses (family filoviridae, order mononegavirales) cause often fatal, haemorrhagic fever in primates including humans. pigs have been identified as a species susceptible to reston ebolavirus (restv) infection, with indicated transmission to humans in the philippines; however, their role during ebola outbreaks in africa needs to be clarified. to perform surveillance studies, detection of ebolavirus requires a prerequisite validation of viral rna and antibody detection methods in swine sampl ... | 2017 | 28345293 |
| reprint of "echinococcus granulosus sensu stricto (s.s.) from the critically endangered antelope addax nasomaculatus in tunisia". | echinococcus granulosus sensu lato (s.l.) is a zoonotic disease highly endemic in tunisia. canids including stray and semi-stray dogs, jackals and foxes are known as definitive hosts and a wide range of ungulates have been shown to harbour the metacestode hydatid stage and may serve as intermediate hosts. fertile hydatid cysts of echinococcus equinus and e. granulosus sensu stricto (s.s.) were recently molecularly identified for the first time from tunisian donkeys. e. granulosus (s.s.) was also ... | 2017 | 27887695 |
| [molecular characterization of echinococcus granulosus isolates obtained from different hosts]. | echinococcus granulosus is a parasite that can be seen throughout the world. so far, five species of genus echinococcus have been identified as parasite in people: e.granulosus, e.multilocularis, e.vogeli, e.oligarthrus, e.shiquicus. larval (metacestod) form of parasite settles in internal organs of hoofed animals (cattle, goats, pigs, horses, sheep, etc.) and human; the adult form is found in small intestine of final host, canine. disease caused by parasite called as "cystic echinococcosis" (ce ... | 2017 | 28283013 |
| implementation of new tools in molecular epidemiology studies of echinococcus granulosus sensu lato in south america. | the aim of this work was to determine echinococcus granulosus sensu lato species and genotypes in intermediate and definitive hosts and in human isolates from endemic regions of argentina and brazil including those where no molecular data is available by a combination of classical and alternative molecular tools. a total of 227 samples were isolated from humans, natural intermediate and definitive hosts. amplification of cytochrome c oxidase subunit i gene fragment was performed and a combinatio ... | 2017 | 28193534 |
| detection of echinococcus granulosus g3 in a wild boar ( sus scrofa ) in central italy using pcr and sequencing. | we report cystic echinococcosis in a free-living wild boar ( sus scrofa ) in europe. parasites were identified by histopathology and molecular techniques, revealing echinococcus granulosus of the g3 genotype. | 2017 | 28118555 |
| harmonizing methods for wildlife abundance estimation and pathogen detection in europe-a questionnaire survey on three selected host-pathogen combinations. | the need for wildlife health surveillance as part of disease control in wildlife, domestic animals and humans on the global level is widely recognized. however, the objectives, methods and intensity of existing wildlife health surveillance programs vary greatly among european countries, resulting in a patchwork of data that are difficult to merge and compare. this survey aimed at evaluating the need and potential for data harmonization in wildlife health in europe. the specific objective was to ... | 2017 | 28202055 |
| investigation on the zoonotic trematode species and their natural infection status in huainan areas of china. | to investigate the species of zoonotic trematodes and the endemic infection status in the domestic animals in huainan areas, north anhui province of china, we intent to provide evidences for prevention of the parasitic zoonoses. | 2017 | 28244789 |
| an indirect enzyme-linked immunosorbent assay for the identification of antibodies to senecavirus a in swine. | senecavirus a (sva), a member of the family picornaviridae, genus senecavirus, is a recently identified single-stranded rna virus closely related to members of the cardiovirus genus. sva was originally identified as a cell culture contaminant and was not associated with disease until 2007 when it was first observed in pigs with idiopathic vesicular disease (ivd). vesicular disease is sporadically observed in swine, is not debilitating, but is significant due to its resemblance to foreign animal ... | 2017 | 28202026 |
| development of a rapid identification method for the differentiation of enterococcus species using a species-specific multiplex pcr based on comparative genomics. | enterococci are lactic acid bacteria that are commonly found in food and in animal gut. since 16 s ribosomal rna (rrna) sequences, genetic markers for bacterial identification, are similar among several enterococcus species, it is very difficult to determine the correct species based on only 16 s rrna sequences. therefore, we developed a rapid method for the identification of different enterococcus species using comparative genomics. we compared 38 genomes of 13 enterococcus species retrieved fr ... | 2017 | 28229213 |
| detection of novel oxazolidinone and phenicol resistance gene optra in enterococcal isolates from food animals and animal carcasses. | altogether 7720 enterococcus faecalis and 3939 e. faecium isolated from food animals and animal carcasses during 2003-2014 in korea were investigated to determine if linezolid-resistant (lr) enterococci (≥8μg/ml) are present. overall, 12 e. faecalis and 27 e. faecium recovered from chickens (n=32), pigs (n=6), and cattle (n=1) were resistant to linezolid and were further characterized using molecular methods most lr isolates were also resistant to chloramphenicol (97.44%) and florfenicol (92.31% ... | 2017 | 28284617 |
| microbial shifts in the porcine distal gut in response to diets supplemented with enterococcus faecalis as alternatives to antibiotics. | gut microbiota plays an important role in host health and nutrient digestion of animals. probiotics have become one of effective alternatives to antibiotics enhancing animal health and performance through modulating gut microbiota. previously, our research demonstrated that dietary enterococcus faecalis uc-100 substituting antibiotics enhanced growth and health of weaned pigs. to investigate the alterations of microbiota in the distal gut of pigs fed e. faecalis uc-100 substituting antibiotics, ... | 2017 | 28165001 |
| integrated multivariate analysis with nondetects for the development of human sewage source-tracking tools using bacteriophages of enterococcus faecalis. | we developed sewage-specific microbial source tracking (mst) tools using enterococci bacteriophages and evaluated their performance with univariate and multivariate analyses involving data below detection limits. newly isolated enterococci faecalis bacterial strains aim06 (dsm100702) and sr14 (dsm100701) demonstrated 100% specificity and 90% sensitivity to human sewage without detecting 68 animal manure pooled samples of cats, chickens, cows, dogs, ducks, pigs, and pigeons. aim06 and sr14 bacter ... | 2017 | 27983829 |
| probiotic properties and adsorption of enterococcus faecalis psct3-7 to vermiculite. | the probiotic properties of enterococcus (e.) faecalis psct3-7, a new strain isolated from the intestines of pigs fed dietary fiber containing 50% sawdust, were investigated. e. faecalis psct3-7 tolerated a ph range of 3 to 8 and 0.3% bile salts, and it inhibited the growth of salmonella typhimurium in a concentration-dependent manner. in addition, e. faecalis showed resistance to several antibacterial agents. vermiculite, a nutrient and microbial carrier, increased the bile tolerance of the str ... | 2017 | 27456777 |
| effects of ex vivo infection with etec on jejunal barrier properties and cytokine expression in probiotic-supplemented pigs. | enterotoxigenic escherichia coli (etec) strains are involved in piglet post-weaning diarrhea. prophylactic measures including probiotics have been examined in infection experiments with live piglets. in the present study, we have tested whether the early effects of etec infection can also be evoked and studied in a model in which etec is added to whole mucosal tissues ex vivo, and whether this response can be modulated by prior supplementation of the piglets with probiotics. | 2017 | 27995406 |
| zoonotic potential of enterocytozoon genotypes in humans and pigs in thailand. | enterocytozoon bieneusi is an opportunistic intestinal pathogen infecting humans and a variety of animals. its mode of transmission and zoonotic potential are not completely understood. e. bieneusi has been frequently identified in pigs. the objective of our study was to investigate e. bieneusi in pigs and humans in western and central thailand to determine its presence, genetic diversity, and zoonotic potential. a total of 277 human and 210 pig faecal samples were collected and analysed. e. bie ... | 2017 | 28043391 |
| structural biology of the arterivirus nsp11 endoribonucleases. | endoribonuclease (nendou) is unique and conserved as a major genetic marker in nidoviruses that infect vertebrate hosts. arterivirus nonstructural protein 11 (nsp11) was shown to have nendou activity and play essential roles in the viral life cycle. here, we report three crystal structures of porcine reproductive and respiratory syndrome virus (prrsv) and equine arteritis virus (eav) nsp11 mutants. the structures of arterivirus nsp11 contain two conserved compact domains: the n-terminal domain ( ... | 2017 | 27795409 |
| single nucleotide polymorphism genotyping of erysipelothrix rhusiopathiae isolates from pigs affected with chronic erysipelas in japan. | over the past decades, erysipelothrix rhusiopathiae strains displaying similar phenotypic and genetic profiles of the attenuated, acriflavine-resistant e. rhusiopathiae koganei 65-0.15 strain (serovar 1a) have been frequently isolated from pigs affected with chronic erysipelas in japan. in this study, using the conventional pcr assay that was designed to detect strain-specific single nucleotide polymorphism (snp) sites found in the genome of the vaccine strain, we analyzed e. rhusiopathiae isola ... | 2017 | 28250289 |
| pathogenic characterization of erysipelothrix rhusiopathiae met-203 type spaa strains from chronic and subacute swine erysipelas in japan. | to characterize the erysipelothrix rhusiopathiae met-203 type surface protective antigen (spa) a strains causing swine erysipelas in japan, the nucleotide sequence of the hypervariable region of the spaa gene was determined in 80 e. rhusiopathiae (serotype 1a) isolates collected from pigs with chronic and subacute swine erysipelas in 14 prefectures in 2008-2014. in this study, 14 (17.5%) isolates were met-203 type spaa strains. we confirmed the pathogenicity of a met-203 type spaa strain in spec ... | 2017 | 27773881 |
| prevalence of diarrheagenic escherichia coli in foods and fecal specimens obtained from cattle, pigs, chickens, asymptomatic carriers, and patients in osaka and hyogo, japan. | the source and routes of diarrheagenic escherichia coli (dec) remain poorly clarified. to investigate the involvement of domestic animals in the dissemination of dec, the prevalence of dec among foods and fecal specimens of cattle, pigs, chickens, healthy carriers, and patients in osaka and hyogo, japan, was investigated using a multiplex real-time pcr assay. the most prevalent virulence genes were asta and eae, with prevalences of 46.8% and 27.4%, respectively. stx1 (26.6%) and stx2 (45.9%) wer ... | 2017 | 28367884 |
| high frequency of b2 phylogroup among non-clonally related fecal escherichia coli isolates from wild boars, including the lineage st131. | wild boars are worldwide distributed mammals which population is increasing in many regions, like the iberian peninsula, leading to an increased exposition to humans. they are considered reservoirs of different zoonotic pathogens and have been postulated as potential vectors of antimicrobial-resistant (amr) bacteria. this study aimed to determine the prevalence of antimicrobial resistance and phylogenetic distribution of escherichia coli from wild boar feces. antimicrobial resistance and integro ... | 2017 | 28365752 |
| tspka-r: a potential immunodiagnostic antigen for the detection of porcine cysticercosis. | cysticercosis, caused by metacestodes of taenia solium, has a significant soci-economic impact and is of considerable importance in public health. however, there are no specific diagnostic antigens to distinguish between t. solim and taenia hydatigena. in the present study, camp-dependent protein kinase regulatory subunit (tspka-r), an excretory/secretary (es) antigen of t. solium, was used to establish a specific and sensitive diagnostic tool for detection of porcine cysticercosis. the full-len ... | 2017 | 28359828 |
| olive oil bioactives protect pigs against experimentally-induced chronic inflammation independently of alterations in gut microbiota. | subclinical chronic inflammation (sci) is associated with impaired animal growth. previous work has demonstrated that olive-derived plant bioactives exhibit anti-inflammatory properties that could possibly counteract the growth-depressing effects of sci. to test this hypothesis and define the underlying mechanism, we conducted a 30-day study in which piglets fed an olive-oil bioactive extract (obe) and their control counterparts (c+) were injected repeatedly during the last 10 days of the study ... | 2017 | 28346507 |
| recombinant expression of porcine spermadhesin awn and its phospholipid interaction: indication for a novel lipid binding property. | awn is a porcine (sus scrofa domestica) seminal plasma protein and has been linked to a variety of processes related to fertilization. to acquire the protein in sufficient amount and purity for functional studies, we established its recombinant expression in e. coli and a three-step purification protocol based on different chromatographies. the test for awn-phospholipid interaction revealed phosphatidic acid and cardiolipin as potential binding partners. as phosphatidic acid is surmised to play ... | 2017 | 28326614 |
| risk factors for esbl-producing escherichia coli on pig farms: a longitudinal study in the context of reduced use of antimicrobials. | the presence of extended-spectrum beta-lactamase-producing escherichia coli (esbl-e. coli) in food animals is a public health concern. this study aimed to determine prevalence of esbl-e. coli on pig farms and to assess the effect of reducing veterinary antimicrobial use (amu) and farm management practices on esbl-e. coli occurrence on pig farms. during 2011-2013, 36 dutch conventional pig farms participated in a longitudinal study (4 sampling times in 18 months). rectal swabs were taken from 60 ... | 2017 | 28323856 |
| e. coli reca gene improves gene targeted homologous recombination in mycoplasma hyorhinis. | mycoplasma hyorhinis is an opportunistic pathogen of pigs. recently, it has been shown to transform cell cultures, increasing the attention of the researchers. studies on the pathogenesis require specific genetic tool that is not yet available for the pathogen. to address this limitation, we constructed two suicide plasmids pgemt-tetm/lr and pgemt-reca-tetm/lr having a tetracycline resistance marker flanked by two hemolysin gene arms. the latter plasmid encodes an e. coli reca, a gene involved i ... | 2017 | 28285864 |
| air exposure as a possible route for esbl in pig farmers. | livestock can carry extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae, with blactx-m-1 being most prevalent. esbl carriage in farmers is associated with esbl carriage in animals, with direct animal-human contact considered as the dominant route of transmission. however, inhalation of stable air might represent another route of transmission. we, therefore, quantified presence of blactx-m group 1 genes (ctx-m-gr1) in dust and the association with ctx-m-gr1 carriage in pig farmer ... | 2017 | 28273621 |
| ampk/α-ketoglutarate axis regulates intestinal water and ion homeostasis in young pigs. | water and ion absorption via sensitive aquaporins (aqps) and ion channels is of critical importance in intestinal health. however, whether α-ketoglutarate (akg) could improve intestinal water and ion homeostasis in lipopolysaccharide (lps)-challenged piglets and whether the amp-activated protein kinase (ampk) pathway is involved remains largely unknown. this study was conducted to investigate the effect of dietary akg supplementation on the small intestinal water and ion homeostasis through modu ... | 2017 | 28241728 |
| effects of essential oil (blended and single essential oils) on anti-biofilm formation of salmonella and escherichia coli. | biofilms were the third-dimensional structure in the solid surface of bacteria. bacterial biofilms were difficult to control by host defenses and antibiotic therapies. escherichia coli (e. coli) and salmonella were popular pathogenic bacteria that live in human and animal intestines. essential oils are aromatic oily liquids from plant materials and well known for their antibacterial activities. | 2017 | 28239484 |
| pathogenic potential and virulence genotypes of intestinal and faecal isolates of porcine post-weaning enteropathogenic escherichia coli. | enteropathogenic escherichia coli (epec) are frequent causes of diarrhoea in infants and in young mammals by inducing attaching effacing (ae) lesions of the intestinal epithelium. epec bacteria have also been implicated in cases of porcine post-weaning diarrhoea but their pathogenicity for conventional weaned pigs remains less elucidated. this present study investigates differences in pathogenic potential and virulence genotypes of intestinal and faecal isolates of epec from newly-weaned pigs. f ... | 2017 | 28231471 |
| human influence and biotic homogenization drive the distribution of escherichia coli virulence genes in natural habitats. | cattle are the main reservoirs for shiga-toxin-producing escherichia coli (stec), the only known zoonotic intestinal e. coli pathotype. however, there are other intestinal pathotypes that can cause disease in humans, whose presence has been seldom investigated. thus, our aim was to identify the effects of anthropic pressure and of wild and domestic ungulate abundance on the distribution and diversity of the main human e. coli pathotypes and nine of their representative virulence genes (vgs). we ... | 2017 | 28213899 |
| the effects of amoxicillin treatment of newborn piglets on the prevalence of hernias and abscesses, growth and ampicillin resistance of intestinal coliform bacteria in weaned pigs. | this study investigated the effects of a single amoxicillin treatment of newborn piglets on the prevalence of hernias and abscesses until the age of nine weeks. we also studied whether the treatment was associated with growth and mortality, the need for treatment of other diseases, the proportions of ampicillin resistant coliforms and antimicrobial resistance patterns of intestinal escherichia coli (e. coli). a total of 7156 piglets, from approximately 480 litters, were divided into two treatmen ... | 2017 | 28199379 |
| effects of armillariella tabescens mycelia on the growth performance and intestinal immune response and microflora of early-weaned pigs. | this study was performed to evaluate effects of armillariella tabescens (a. tabescens) on the growth performance and intestinal immune response and microflora in early-weaned pigs when used as feed additive. a. tabescens mycelia were added to basal diets at concentrations of 0%, 0.1%, 0.3% or 0.9% (w/w). a total of 144 commercial cross-bred piglets were randomly allocated to one of these four diets and fed for 30 days. the growth performance of early-weaned piglets displayed improvement with die ... | 2017 | 28183153 |
| cranberry extract inhibits in vitro adhesion of f4 and f18(+)escherichia coli to pig intestinal epithelium and reduces in vivo excretion of pigs orally challenged with f18(+) verotoxigenic e. coli. | f4(+)e. coli and f18(+)e. coli infections are an important threat for pig industry worldwide. antibiotics are commonly used to treat infected piglets, but the emerging development of resistance against antibiotics raises major concerns. hence, alternative therapies to prevent pigs from f4(+)e. coli and f18(+)e. coli infections need to be developed. since cranberry previously showed anti-adhesive activity against uropathogenic e. coli, we aimed to investigate whether cranberry extract could also ... | 2017 | 28161211 |
| improved porcine model for shiga toxin-producing escherichia coli infection by deprivation of colostrum feeding in newborn piglets. | porcine edema disease (ed) is a toxemia caused by enteric infection with shiga toxin 2e (stx2e)-producing escherichia coli (stec). ed occurs most frequently during the weaning period and is manifested as emaciation associated with high mortality. in our experimental infection with a specific stec strain, we failed to cause the suppression of weight gain in piglets, which is a typical symptom of ed, in two consecutive experiments. therefore, we examined the effects of deprivation of colostrum on ... | 2017 | 28145027 |
| environmental emission of multiresistant escherichia coli carrying the colistin resistance gene mcr-1 from german swine farms. | pigs have been the focus of the worldwide spread of colistin resistance. however, there is little information on the transmission of mcr-1-containing bacteria into the environment of pig farms. we therefore rescreened environmental escherichia coli isolates from the surrounding farm areas of three previously mcr-1-positive swine herds in germany. | 2017 | 28122910 |
| comparative pharmacokinetics of eight major bioactive components in normal and bacterial diarrhea mini-pigs after oral administration of gegen qinlian decoction. | healthy animals are most widely used in current pharmacokinetic(pk) studies. however, neglecting the effects of specific diseases on drug absorption results in the pk parameters of those experiments not accurately reflecting in vivo drug concentration changes during treatment. in this study, an e. coli infective diarrheal minipig model was applied to explore the pharmacokinetics of gegen qinlian decoction (gqd). a simple and rapid ultrahigh performance liquid chromatography-tandem mass spectrome ... | 2017 | 28107700 |
| microbial source tracking in shellfish harvesting waters in the gulf of nicoya, costa rica. | current microbial water quality monitoring is generally limited to culture-based measurements of fecal indicator bacteria (fib). given the many possible sources of fecal pollution within a watershed and extra-intestinal fib reservoirs, it is important to determine source(s) of fecal pollution as a means to improve water quality and protect public health. the principal objective of this investigation was to characterize the microbial water quality of shellfish harvesting areas in the gulf of nico ... | 2017 | 28086114 |
| the fecal presence of enterotoxin and f4 genes as an indicator of efficacy of treatment with colistin sulfate in pigs. | enterotoxigenic escherichia coli (etec) strains producing multiple enterotoxins are important causes of post-weaning diarrhea (pwd) in pigs. the aim of the present study was to investigate the fecal presence of etec enterotoxin as well as f4 and f18 genes as an indicator of colistin sulfate (cs) efficacy for treatment of pwd in pigs. forty-eight piglets were weaned at the age of 21 days, and were divided into four groups: challenged treated, challenged untreated, unchallenged treated, and unchal ... | 2017 | 28056796 |
| genotype variation and genetic relationship among escherichia coli from nursery pigs located in different pens in the same farm. | so far, little is known about the genetic diversity and relatedness among escherichia coli (e. coli) populations in the gut of swine. information on this is required to improve modeling studies on antimicrobial resistance aiming to fight its occurrence and development. this work evaluated the genotype variation of e. coli isolated from swine fecal samples at the single pig and pen level, as well as between pens using repetitive extragenic palindromic (rep) pcr fingerprinting and pulsed field gel ... | 2017 | 28056788 |
| [serotype identification and antibiotic susceptibility of shiga toxin-producing escherichia coli in the weishan area in shandong province, china]. | objective: to determine the serotypes and drug resistance profiles of shiga toxin-producing escherichia coli (stec) in animal stools from the weishan area in shandong province, china. to provide the basis for further study. methods: five hundred animal stool samples (from pigs, cattle, sheep, dogs and birds) were collected from the weishan area and stec strains were isolated from these samples. strains were serotyped by a serum agglutination test, and their drug resistance profiles were determin ... | 2017 | 28056274 |
| antimicrobial susceptibility and genetic characterization of escherichia coli recovered from frozen game meat. | the increasing number of antimicrobial resistant enterobacteriaceae both in veterinary and human medicine, the dissemination of these bacteria in several environments and their possible repercussions on human health is causing concern. game meat is usually seen as free of antimicrobial resistant bacteria. the objective of this study was to evaluate the current antimicrobial susceptibility status in generic escherichia coli isolated from packed frozen game meat from a game handling establishment ... | 2017 | 28040165 |
| immunogenicity and protective efficacy of a single-dose live non-pathogenic escherichia coli oral vaccine against f4-positive enterotoxigenic escherichia coli challenge in pigs. | enterotoxigenic escherichia coli strains expressing f4 (k88) fimbriae (f4-etec) are one of the most important causes of post-weaning diarrhea (pwd) in pigs. f4, a major antigen, plays an important role in the early steps of the infection. herein, the efficacy of a live oral vaccine consisting of a non-pathogenic e. coli strain expressing f4 for protection of pigs against pwd was evaluated. three blinded, placebo-controlled, block design, parallel-group confirmatory experiments were conducted, us ... | 2017 | 27916413 |
| differential effect of early antibiotic intervention on bacterial fermentation patterns and mucosal gene expression in the colon of pigs under diets with different protein levels. | the study aimed to evaluate the effects of early antibiotic intervention (eai) on bacterial fermentation patterns and mucosal immune markers in the colon of pigs with different protein level diets. eighteen litters of piglets at day (d) 7 were fed creep feed without or with growth promoting antibiotics until d 42. at d 42, pigs within each group were further randomly assigned to a normal- or low-crude protein (cp) diet. at d 77 and d 120, five pigs per group were slaughtered for analyzing coloni ... | 2017 | 27913852 |
| high rates of ctx-m-15-producing escherichia coli and klebsiella pneumoniae in wild boars and barbary macaques in algeria. | the present study aimed to screen for the presence of extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae in wild boars and barbary macaques in béjaïa and jijel, algeria. | 2017 | 27984780 |
| escherichia coli probiotic strain ed1a in pigs has a limited impact on the gut carriage of extended-spectrum-β-lactamase-producing e. coli. | four trials were conducted to evaluate the impact of escherichia coli probiotic strain ed1a administration to pigs on the gut carriage or survival in manure of extended-spectrum-β-lactamase-producing e. coli groups of pigs were orally inoculated with strain e. coli m63 carrying the blactx-m-1 gene (n = 84) or used as a control (n = 26). in the first two trials, 24 of 40 e. coli m63-inoculated pigs were given e. coli ed1a orally for 6 days starting 8 days after oral inoculation. in the third tria ... | 2017 | 27795372 |
| antimicrobial peptides of meat origin - an in silico and in vitro analysis. | the aim of this study was to evaluate the antimicrobial activity of meat protein-derived peptides against selected gram-positive and gram-negative bacteria. the in silico and in vitro approach was combined to determine the potency of antimicrobial peptides derived from pig (sus scrofa) and cow (bos taurus) proteins. the in silico studies consisted of an analysis of the amino acid composition of peptides obtained from the campr database, their molecular weight and other physicochemical properties ... | 2017 | 28000569 |
| occurrence and characterization of mcr-1-harbouring escherichia coli isolated from pigs in great britain from 2013 to 2015. | to determine the occurrence of mcr-1 -harbouring escherichia coli in archived pig material originating in great britain (gb) from 2013 to 2015 and characterize mcr-1 plasmids. | 2017 | 27999032 |
| antimicrobial susceptibility and phylotyping profile of pathogenic escherichia coli and salmonella enterica isolates from calves and pigs in minas gerais, brazil. | the aims of the present study were to determine (i) the profiles of phylogroup and (ii) the antimicrobial susceptibility of pathogenic escherichia coli strains isolated from calves, and of salmonella spp. strains isolated from calves and pigs in minas gerais state, brazil. sixty-one pathogenic e. coli strains and salmonella spp. (n = 24) strains isolated from fecal samples of calves and salmonella spp. (n = 39) strains previously isolated from fecal samples of growing/finishing pigs were tested. ... | 2017 | 27709395 |
| extended-spectrum β-lactamase (esbl)-producing escherichia coli isolates collected from diseased food-producing animals in the germ-vet monitoring program 2008-2014. | the aim of this study was to identify extended-spectrum β-lactamase (esbl)-producing escherichia coli collected from diseased food-producing animals in germany. a total of 6849 e. coli isolates, collected from diseased cattle, pigs and poultry in the german national monitoring program germ-vet (2008-2014), were characterized by antimicrobial susceptibility testing and screened for the esbl phenotype. esbl genes were identified by pcr and sequencing. the isolates were further characterized by pcr ... | 2017 | 27634182 |
| identification, characterization and bioactivity of tumor necrosis factor (tnf)-related apoptosis-inducing ligand from equus caballus. | tumor necrosis factor (tnf)-related apoptosis-inducing ligand (trail) is a member of the tnf superfamily and plays multifunctional roles in the immune system. in the present study, a homolog of trail from the mongolian horse (named ectrail) was identified and characterized. the 870-bp open reading frame encodes a polypeptide of 289 amino acid residues with a predicted molecular weight of 33.47 kda and pi of 8.47. the genomic structure of ectrail shares a five-exon/six-intron arrangement similar ... | 2017 | 27592050 |
| effects of dietary supplementation with freshwater microalgae on growth performance, nutrient digestibility and gut health in weaned piglets. | in pigs, digestive disorders associated with weaning lead to antibiotic use to maintain intestinal health. microalgae have been studied in humans and rodents for their beneficial effects on health. the nutritional value of microalgae in animal diets has been assessed, but results were not conclusive. dietary supplementation with microalgae as an alternative to antibiotic use was studied in two trials (72 piglets with initial bw=9.1±1.1 kg in trial 1 and 24 piglets with initial bw=9.1±0.9 kg in t ... | 2017 | 27452961 |
| the effect of different gas permeability of packaging on physicochemical and microbiological parameters of pork loin storage under high o2 modified atmosphere packaging conditions. | the aim of this study was to determine the influence of different packaging materials on meat quality during cold storage. therefore pork loins (m. longissimus thoracis et lumborum) obtained from crossbred pigs (polish landrance x duroc, n = 6) were stored at 2 ℃ in modified atmosphere packs (80% o2, 20% co2) in four types of trays, which differ in gas permeability. physicochemical (headspace gas composition, ph, colour, drip loss, cooking loss, shear force, the basic composition and fatty acid ... | 2017 | 27688300 |
| effect of feed supplementation with live yeast on the intestinal transcriptome profile of weaning pigs orally challenged with escherichia coli f4. | the ability of live yeasts to modulate pig intestinal cell signals in response to infection with escherichia coli f4ac (etec) has not been studied in-depth. the aim of this trial was to evaluate the effect of saccharomyces cerevisiae cncm i-4407 (sc), supplied at different times, on the transcriptome profile of the jejunal mucosa of pigs 24 h after infection with etec. in total, 20 piglets selected to be etec-susceptible were weaned at 24 days of age (day 0) and allotted by litter to one of foll ... | 2017 | 27358089 |
| effects of dietary supplementation of bacteriophages against enterotoxigenic escherichia coli (etec) k88 on clinical symptoms of post-weaning pigs challenged with the etec pathogen. | the present study was performed to investigate the effects of dietary supplementation of bacteriophages (phages) against enterotoxigenic escherichia coli (etec) k88 as a therapy against the etec infection in post-weaning pigs. two groups of post-weaning pigs aged 35 days, eight animals per group, were challenged with 3.0 × 10(10) colony forming units of etec k88, a third group given the vehicle. the unchallenged group and one challenged group were fed a basal nursery diet for 14 days while the r ... | 2017 | 27271838 |
| risk factors for antimicrobial resistance in escherichia coli in pigs receiving oral antimicrobial treatment: a systematic review. | the aim of this literature review was to identify risk factors in addition to antimicrobial treatment for antimicrobial resistance (amr) occurrence in commensal escherichia coli in pigs. a variety of studies were searched in 2014 and 2015. studies identified as potentially relevant were assessed against eligibility criteria such as observation or experiment (no review), presentation of risk factors in addition to (single dosage) antimicrobial use, risk factors for but not resulting from amr, and ... | 2017 | 27249658 |
| α1,2-fucosyllactose does not improve intestinal function or prevent escherichia coli f18 diarrhea in newborn pigs. | infectious diarrhea, a leading cause of morbidity and deaths, is less prevalent in breastfed infants compared with infants fed infant formula. the dominant human milk oligosaccharide (hmo), α-1,2-fucosyllactose (2'-fl), has structural homology to bacterial adhesion sites in the intestine and may in part explain the protective effects of human milk. we hypothesized that 2'-fl prevents diarrhea via competitive inhibition of pathogen adhesion in a pig model for sensitive newborn infants. | 2017 | 27243420 |
| effects of ecklonia cava as fucoidan-rich algae on growth performance, nutrient digestibility, intestinal morphology and caecal microflora in weanling pigs. | in the present study, role of increasing levels of ecklonia cava (seaweed) supplementation in diets was investigated on growth performance, coefficient of total tract apparent digestibility (cttad) of nutrients, serum immunoglobulins, cecal microflora and intestinal morphology of weanling pigs. | 2017 | 27165019 |
| effects of lactobacillus acidophilus supplementation on growth performance, nutrient digestibility, fecal microbial and noxious gas emission in weaning pigs. | antibiotics used as growth promoters in livestock have been banned in the european union since 2006. antibiotics alternatives have focused on probiotics, such as lactobacillus acidophilus. the concentration of l. acidophilus is considered crucial for obtaining the desired effects. however, limited studies have been conducted to test the dose-dependent effects of l. acidophilus. therefore, the present study aimed to test the dose-dependent effects of l. acidophilus on growth performance, nutrient ... | 2017 | 27342084 |
| a review of the current status of relevant zoonotic pathogens in wild swine (sus scrofa) populations: changes modulating the risk of transmission to humans. | many wild swine populations in different parts of the world have experienced an unprecedented demographic explosion that may result in increased exposure of humans to wild swine zoonotic pathogens. interactions between humans and wild swine leading to pathogen transmission could come from different ways, being hunters and game professionals the most exposed to acquiring infections from wild swine. however, increasing human settlements in semi-natural areas, outdoor activities, socio-economic cha ... | 2017 | 25953392 |
| experimental evaluation of faecal escherichia coli and hepatitis e virus as biological indicators of contacts between domestic pigs and eurasian wild boar. | domestic pigs and eurasian wild boar (sus scrofa) share several important viral and bacterial pathogens. therefore, direct and indirect contacts between domestic pigs and wild boar present a risk of pathogen spillover and can lead to long-term perpetuation of infection. biological indicators could be a powerful tool to understand and characterize contacts between wild boar and domestic pigs. here, faecal escherichia coli and hepatitis e virus (hev) were explored as potential biological indicator ... | 2017 | 26190581 |
| genes encoding homologous antigens in taeniid cestode parasites: implications for development of recombinant vaccines produced in escherichia coli. | recombinant vaccine antigens are being evaluated for their ability to protect livestock animals against cysticercosis and related parasitic infections. practical use of some of these vaccines is expected to reduce parasite transmission, leading to a reduction in the incidence of neurocysticercosis and hydatid disease in humans. we recently showed that an antigen (tsol16), expressed in escherichia coli, confers high levels of protection against taenia solium cysticercosis in pigs, which provides ... | 2017 | 23090389 |
| the global seroprevalence of toxoplasma gondii among wild boars: a systematic review and meta-analysis. | this systematic review and meta-analysis study was performed to evaluate the worldwide seroprevalence of toxoplasma gondii among wild boar. we searched pubmed, science direct, web of science, cochrane, scopus, ebscohost and google scholar databases for studies reporting t. gondii seroprevalence in wild boars between january 1995 and march 2017. inclusion and exclusion criteria were applied. we estimated the pooled seroprevalence of t. gondii in wild boars using a random-effects model, and evalua ... | 2017 | 28917302 |
| [purification, identification and characterization of an anti-microbial hexapeptide from sus scrofa lysozyme]. | sus scrofa lysozyme (ssl) was digested by different proteases to find peptides with enhanced antibacterial activity against gram-negative bacteria. hydrolysate with the highest anti-bacterial activity was loaded onto a gel filtration chromatography column followed by a reversed-phase one. the obtained substance was identified by liquid chromatography-mass spectrometry, synthesized to test its antibacterial spectrum and analyzed for bioinformatics. the hydrolysate of trypsin showed the highest an ... | 2017 | 28895366 |
| selection of invasive wild pig countermeasures using multicriteria decision analysis. | wild pigs are a widespread invasive species that pose significant environmental and social risks. a number of wild pig eradication and control measures exist, but many eradication campaigns are ultimately unsuccessful. decision making regarding how to design and execute an eradication plan is difficult because of multiple costs and benefits spanning various decision criteria that are associated with different eradication and control countermeasures. moreover, multiple stakeholders are often invo ... | 2017 | 27744261 |
| comprehensive variation discovery and recovery of missing sequence in the pig genome using multiple de novo assemblies. | uncovering genetic variation through resequencing is limited by the fact that only sequences with similarity to the reference genome are examined. reference genomes are often incomplete and cannot represent the full range of genetic diversity as a result of geographical divergence and independent demographic events. to more comprehensively characterize genetic variation of pigs (sus scrofa), we generated de novo assemblies of nine geographically and phenotypically representative pigs from eurasi ... | 2017 | 27646534 |
| ecology: invasive wild pigs spread across us. | 2017 | 28079065 | |
| molecular cloning and characterization of porcine ribosomal protein l21. | ribosomal protein l21 (rpl21) is a structural component of the 60s subunit of the eukaryotic ribosome. this protein has an important role in protein synthesis and the occurrence of hereditary diseases. pig is a common laboratory model, however, to the best of our knowledge, itsrpl21gene has not been cloned to date. in this study, we cloned and identified the full-length sequence of the pigrpl21gene for the first time. in addition, we examined its expression pattern and function by using overexpr ... | 2017 | 28057907 |
| enhancing the antimicrobial activity of sus scrofa lysozyme by n-terminal fusion of a sextuple unique homologous peptide. | sus scrofa lysozyme (ssl), an important component of the pig immune system, is a potential candidate to replace antibiotics in feed. however, there is little antimicrobial activity of natural ssl against gram-negative bacteria, which limits its application. in this study, a unique peptide (a-w-v-a-w-k) with antimicrobial activity against gram-negative bacteria was discovered and purified from trypsin hydrolysate of natural ssl. this unique peptide was fused to natural ssl and the recombinant fus ... | 2017 | 28034616 |
| inner ear development in cetaceans. | cetaceans face the challenge of maintaining equilibrium underwater and obtaining sensory input within a dense, low-visibility medium. the cetacean ear represents a key innovation that marked their evolution from terrestrial artiodactyls to among the most fully aquatic mammals in existence. using micro-ct and histological data, we document shape and size changes in the cetacean inner ear during ontogeny, and demonstrate that, as a proportion of gestation time, the cetacean inner ear is precocial ... | 2017 | 27995620 |
| taphonomic effects of mechanical plowing on buried juvenile-sized remains. | agricultural activity is a worldwide taphonomic process and can present unique challenges in the recovery of buried remains. previous research has been mostly within the realm of site formation processes of archeological sites utilizing only surface material. this research expands upon the previous research by incorporating the distribution of subsurface material by the use of archeological excavation techniques. an experiment was conducted utilizing juvenile pig (sus scrofa) skeletons buried in ... | 2017 | 27864968 |
| an examination of the transition of fracture characteristics in long bones from fresh to dry in central florida: evaluating the timing of injury. | it is important to conduct timing of injury research analyzing fracture characteristics at known postmortem intervals (pmi) because bone can retain fresh characteristics throughout the pmi. defleshed pig (sus scrofa) long bones were fractured weekly in two environments (full sun and shade) over 14 weeks in central florida and fracture characteristics were categorized (n = 136) for analysis. results of analysis of variance (anova) using time in weeks (pmi) as a dependent variable indicate signifi ... | 2017 | 27864956 |
| effectiveness and practicality of control strategies for african swine fever: what do we really know? | african swine fever (asf) is a major pig health problem, and the causative virus is moving closer to western european regions where pig density is high. stopping or slowing down the spread of asf requires mitigation strategies that are both effective and practical. based on the elicitation of asf expert opinion, this study identified surveillance and intervention strategies for asf that are perceived as the most effective by providing the best combination between effectiveness and practicality. ... | 2017 | 27852963 |
| parasite to patient: a quantitative risk model for trichinella spp. in pork and wild boar meat. | consumption of raw or inadequately cooked pork meat may result in trichinellosis, a human disease due to nematodes of the genus trichinella. in many countries worldwide, individual control of pig carcasses at meat inspection is mandatory but incurs high costs in relation to absence of positive carcasses from pigs reared under controlled housing. eu regulation 2015/1375 implements an alternative risk-based approach, in view of absence of positive findings in pigs under controlled housing conditio ... | 2017 | 27816842 |
| levels of metals in kidney, liver, and muscle tissue and their influence on the fitness for the consumption of wild boar from western slovakia. | due to environmental pollution, wild animals are exposed to various pollutants. some game animals, such as wild boars are used by people for food, but their meat is not evaluated regarding pollution transfer, since they are unavailable on the official market. the aim of this paper is to present the concentrations of chosen metals (cd, co, cu, hg, pb, and zn) in the kidneys, liver, and muscles of wild boars (n = 40) hunted in eastern slovakia, as derivatives of physiological distribution and anth ... | 2017 | 27812912 |
| identification of growth trait related genes in a yorkshire purebred pig population by genome-wide association studies. | the aim of this study is to identify genomic regions or genes controlling growth traits in pigs. | 2017 | 27809465 |
| wild boars as spore dispersal agents of ectomycorrhizal fungi: consequences for community composition at different habitat types. | the success of dispersal events depend on the organism's ability to reach and establish in a new habitat. in symbiotic organisms, establishment also depends on the presence of their symbiont partner in the new habitat. for instance, the establishment of obligate ectomycorrhizal (em) trees outside the forest is largely limited by the presence of em fungi in soil. wild boars (sus scrofa) are important dispersal agents of em fungal spores, particularly in the moderately dry mediterranean region. th ... | 2017 | 27783205 |
| the telencephalon of the göttingen minipig, cytoarchitecture and cortical surface anatomy. | during the last 20 years pigs have become increasingly popular in large animal translational neuroscience research as an economical and ethical feasible substitute to non-human primates. the anatomy of the pig telencephalon is, however, not well known. we present, accordingly, a detailed description of the surface anatomy and cytoarchitecture of the göttingen minipig telencephalon based on macrophotos and consecutive high-power microphotographs of 15 μm thick paraffin embedded nissl-stained coro ... | 2017 | 27778106 |