Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| superinfection interference between dengue-2 and dengue-4 viruses in aedes aegypti mosquitoes. | dengue virus consists of four antigenically distinct serotypes (denv 1-4) that are transmitted to humans by aedes aegypti and aedes albopictus mosquitoes. in many dengue-endemic regions, co-circulation of two or more denv serotypes is fairly common increasing the likelihood for exposure of the two vectors to multiple serotypes. we used a model system of denv-2 and denv-4 to investigate how prior exposure of aedes aegypti to one denv serotype affects its susceptibility to another serotype. | 2017 | 28150899 |
| the transovarial transmission in the dynamics of dengue infection: epidemiological implications and thresholds. | the anthropophilic and peridomestic female mosquito aedes aegypti bites humans to suck blood to maturate fertilized eggs, during which dengue virus can be spread between mosquito and human populations. besides this route of transmission, there is a possibility of dengue virus being passed directly to offspring through transovarial (or vertical) transmission. the effects of both horizontal and transovarial transmission routes on the dengue virus transmission are assessed by mathematical modeling. ... | 2017 | 28131789 |
| recent advances in the identification of the host factors involved in dengue virus replication. | dengue virus (denv) belongs to the genus flavivirus of the family flaviviridae and it is primarily transmitted via aedes aegypti and aedes albopictus mosquitoes. the life cycle of denv includes attachment, endocytosis, protein translation, rna synthesis, assembly, egress, and maturation. recent researches have indicated that a variety of host factors, including cellular proteins and micrornas, positively or negatively regulate the denv replication process. this review summarizes the latest findi ... | 2017 | 28124222 |
| aedes aegypti (diptera: culicidae) longevity and differential emergence of dengue fever in two cities in sonora, mexico. | dengue virus, primarily transmitted by the aedes aegypti (l.) mosquito, has rapidly expanded in geographic extent over the past several decades. in some areas, however, dengue fever has not emerged despite established ae. aegypti populations. the reasons for this are unclear and have sometimes been attributed to socio-economic differences. in 2013 we compared ae. aegypti adult density and population age structure between two cities in sonora, mexico: hermosillo, which has regular seasonal dengue ... | 2017 | 28082648 |
| comparison of stable and transient wolbachia infection models in aedes aegypti to block dengue and west nile viruses. | pathogen replication and transmission in wolbachia infected insects are currently studied using three wolbachia infection systems: naturally infected wolbachia hosts, hosts transinfected with wolbachia (stably maintained and inherited infections) and hosts transiently infected with wolbachia. all three systems have been used to test the effect of wolbachia on mosquito transmitted pathogens such as dengue virus (denv), west nile virus (wnv) and plasmodium. from these studies it is becoming increa ... | 2017 | 28052065 |
| magnetic nanoparticles are highly toxic to chloroquine-resistant plasmodium falciparum, dengue virus (den-2), and their mosquito vectors. | a main challenge in parasitology is the development of reliable tools to prevent or treat mosquito-borne diseases. we investigated the toxicity of magnetic nanoparticles (mnp) produced by magnetospirillum gryphiswaldense (strain msr-1) on chloroquine-resistant (cq-r) and sensitive (cq-s) plasmodium falciparum, dengue virus (den-2), and two of their main vectors, anopheles stephensi and aedes aegypti, respectively. mnp were studied by fourier-transform infrared spectroscopy and transmission elect ... | 2017 | 27815736 |
| an analysis of the potential impact of climate change on dengue transmission in the southeastern united states. | dengue fever, caused by a mosquito-transmitted virus, is an increasing health concern in the americas. meteorological variables such as temperature and precipitation can affect disease distribution and abundance through biophysical impacts on the vector and on the virus. such tightly coupled links may facilitate further spread of dengue fever under a changing climate. in the southeastern united states, the dengue vector is widely established and exists on the current fringe of dengue transmissio ... | 2017 | 27713106 |
| argonaute 2 suppresses japanese encephalitis virus infection in aedes aegypti. | there are three main innate immune mechanisms against viruses in mosquitoes. infection with the flavivirus dengue virus is controlled by rna interference (rnai) and the jak-stat and toll signaling pathways. this study showed that another flavivirus, japanese encephalitis virus (jev), did not invade the salivary glands of aedes aegypti and that this may be a result of the innate immune resistance to the virus. argonaute 2 (ago2) plays a critical role in the rnai pathway. to understand the mechani ... | 2017 | 27169949 |
| predicting arboviral disease emergence using bayesian networks: a case study of dengue virus in western australia. | a bayesian belief network (bbn) for assessing the potential risk of dengue virus emergence and distribution in western australia (wa) is presented and used to identify possible hotspots of dengue outbreaks in summer and winter. the model assesses the probabilities of two kinds of events which must take place before an outbreak can occur: (1) introduction of the virus and mosquito vectors to places where human population densities are high; and (2) vector population growth rates as influenced by ... | 2017 | 27620510 |
| the dna chaperone hmgb1 potentiates the transcriptional activity of rel1a in the mosquito aedes aegypti. | high mobility group protein 1 (hmgb1) is a non-histone, chromatin-associated nuclear protein that functions in regulating eukaryotic gene expression. we investigated the influence and mechanism of action of aedes aegypti hmgb1 (aahmgb1) on mosquito rel1a-mediated transcription from target gene promoters. the dna-binding domain (rhd) of aarel1a was bacterially expressed and purified, and aahmgb1 dramatically enhanced rhd binding to consensus nf-kb/rel dna response elements. luciferase reporter an ... | 2017 | 27867076 |
| molecular identification of aedes aegypti mosquitoes from pilani region of rajasthan, india. | aedes aegypti is the most important vector of dengue virus infection in humans worldwide. accurate identification and colonization are the essential requirements to understand vector biology as well as its diseases transmission potential. in this study, we have used molecular approaches for the identification of ae. aegypti mosquitoes that were collected from the pilani region of rajasthan, india methods: field collected mosquito larvae were colonized under laboratory conditions. conserved genet ... | 2017 | 27353585 |
| potential high-risk areas for zika virus transmission in the contiguous united states. | to understand where transmission of zika virus has the highest likelihood to occur in the contiguous united states with regard to its transmission both sexually and via aedes aegypti mosquito bites. | 2017 | 28323468 |
| chemical composition and in vitro antioxidant, cytotoxic, antimicrobial, and larvicidal activities of the essential oil of mentha piperita l. (lamiaceae). | the essential oil was obtained by hydrodistillation and the identification and quantification of components were achieved with the use of gc-ms analysis. the antioxidant activity was evaluated by the method of sequestration of dpph. essential oils were used for study the cytotoxic front larvae of artemia salina. in the evaluation of the antimicrobial activity of essential oils, we employed the disk-diffusion method. the potential larvicide in mosquito larvae of the third stage of development of ... | 2017 | 28116346 |
| transcriptome sequencing reveals large-scale changes in axenic aedes aegypti larvae. | mosquitoes host communities of microbes in their digestive tract that consist primarily of bacteria. we previously reported that aedes aegypti larvae colonized by a native community of bacteria and gnotobiotic larvae colonized by only escherichia coli develop very similarly into adults, whereas axenic larvae never molt and die as first instars. in this study, we extended these findings by first comparing the growth and abundance of bacteria in conventional, gnotobiotic, and axenic larvae during ... | 2017 | 28060822 |
| development of dirofilaria immitis and dirofilaria repens in aedes japonicus and aedes geniculatus. | the mosquito-borne filarial nematodes dirofilaria immitis and dirofilaria repens primarily affect dogs but also cats, causing heartworm disease or subcutaneous dirofilariosis, respectively, and both may also cause zoonotic diseases in humans. several mosquito species have been reported as competent vectors for these nematodes, but no data are available for the invasive mosquito species aedes japonicus (theobald, 1901). the objective of this study was to describe the development of both d. immiti ... | 2017 | 28219407 |
| mosgctl-7, a c-type lectin protein, mediates japanese encephalitis virus infection in mosquitoes. | japanese encephalitis virus (jev) is an arthropod-borne flavivirus prevalent in asia and the western pacific, and is the leading cause of viral encephalitis. jev is maintained in a transmission cycle between mosquitoes and vertebrate hosts, but the molecular mechanisms by which the mosquito vector participates in transmission are unclear. we investigated the expression of all c-type lectins during jev infection in aedes aegypti. c-type lectin mosgctl-7 (aael002524) was significantly up-regulated ... | 2017 | 28250133 |
| n-glycosylation influences the catalytic activity of mosquito α-glucosidases associated with susceptibility or refractoriness to lysinibacillus sphaericus. | cqm1 and aam1 are α-glucosidases (ec 3.2.1.20) expressed in culex quinquefasciatus and aedes aegypti larvae midgut, respectively. these orthologs share high sequence similarity but while cqm1 acts as a receptor for the binary (bin) insecticidal toxin from lysinibacillus sphaericus, aam1 does not bind the toxin, rendering ae. aegypti refractory to this bacterium. aam1 is heavily glycosylated, contrasting to cqm1, but little is known regarding how glycosylation impacts on its function. this study ... | 2017 | 28017798 |
| first case of zika virus infection in a hiv+ patient. | zika virus is an enveloped, single-stranded rna virus that belongs to the flavivirus genre. as with arbovirus, it is transmitted by arthropods (mosquitoes), both aedes aegypti (urban) and albopictus (rural). zika virus was first isolated in 1947 from monkeys in uganda. the first human cases were reported in 1952 in east africa. outbreaks were reported in micronesia (2007), polynesia (2013), and chile (2014). both diagnostic procedures and therapeutics are poorly developed. serological tests cros ... | 2017 | 27232506 |
| larvicidal and growth-inhibitory activity of entomopathogenic bacteria culture fluids against aedes aegypti (diptera: culicidae). | dengue, chikungunya, and zika are important vector-borne diseases, and aedes aegypti l. is their main transmitter. as the disease management is mainly based on mosquito control strategies, the search for alternative and cost-effective approaches is ongoing. the gram-negative bacteria xenorhabdus nematophila and photorhabdus luminescens are symbiotically associated with entomopathogenic nematodes and are highly pathogenic for insect larvae. after we have recently confirmed the toxicity of these b ... | 2017 | 28062794 |
| the evolutionary divergence of stat transcription factor in different anopheles species. | anopheles mosquito transmits plasmodium, the malaria causing parasite. different species of anopheles mosquito dominate in a particular geographical location and are capable of transmitting specific strains of plasmodium. it is important to understand the biology of different anophelines to control the parasite transmission. stat is an evolutionary conserved transcription factor that regulates the parasite development in african malaria vector anopheles gambiae. unlike drosophila and aedes aegyp ... | 2017 | 27664587 |
| molecular expression of aquaporin mrnas in the northern house mosquito, culex pipiens. | aquaporins (aqps) are a family of membrane-bound proteins, originally described as water-channels, that broadly exist in prokaryotic and eukaryotic cells. however, some aqps can transport small molecules (e.g. urea, glycerol) along with or preferentially to water. previous work in the mosquitoes aedes aegypti and anopheles gambiae have characterized the molecular expression of one or more aqp genes and shown that they are involved in water homeostasis after blood feeding, tolerance to dehydratio ... | 2017 | 27773635 |
| toxicity of herbal extracts used in ethno-veterinary medicine and green-encapsulated zno nanoparticles against aedes aegypti and microbial pathogens. | dengue and chikungunya are arboviral diseases mainly vectored by the mosquito aedes aegypti. presently, there is no treatment for these viral diseases and their prevention is still based on vector control measures. nanopesticides fabricated using herbal extracts as reducing and capping agents currently represent an excellent platform for pest control. in this scenario, the present study assessed the acute toxicity of seven plants employed in ethno-veterinary medicine of southern india, as well a ... | 2017 | 28389893 |
| pseudomonas aeruginosa kun2, extracellular toxins-a potential source for the control of dengue vector. | dengue fever is one of the serious health disease transmitted by aedes spp mosquitoes. the incidence of dengue has increased dramatically around the world in recent decades. vector control is one of the important strategies practiced for the control of dengue fever. the emergence of resistance among vectors against the existing insecticides has raised new challenges. the aim of the present study was to identify the larvicidal activity of extracellular toxins from pseudomonas spp for the control ... | 2017 | 27353579 |
| artesunate-tafenoquine combination therapy promotes clearance and abrogates transmission of the avian malaria parasite plasmodium gallinaceum. | clinical manifestations of malaria infection in vertebrate hosts arise from the multiplication of the asexual stage parasites in the blood, while the gametocytes are responsible for the transmission of the disease. antimalarial drugs that target the blood stage parasites and transmissible gametocytes are rare, but are essentially needed for the effective control of malaria and for limiting the spread of resistance. artemisinin and its derivatives are the current first-line antimalarials that are ... | 2017 | 28043395 |
| microbiota activates imd pathway and limits sindbis infection in aedes aegypti. | aedes aegypti is the main vector of important arboviruses such as dengue, zika and chikungunya. during infections mosquitoes can activate the immune pathways toll, imd and jak/stat to limit pathogen replication. | 2017 | 28231846 |
| modelling wolbachia infection in a sex-structured mosquito population carrying west nile virus. | wolbachia is possibly the most studied reproductive parasite of arthropod species. it appears to be a promising candidate for biocontrol of some mosquito borne diseases. we begin by developing a sex-structured model for a wolbachia infected mosquito population. our model incorporates the key effects of wolbachia infection including cytoplasmic incompatibility and male killing. we also allow the possibility of reduced reproductive output, incomplete maternal transmission, and different mortality ... | 2017 | 28097419 |
| rplp1 and rplp2 are essential flavivirus host factors that promote early viral protein accumulation. | the flavivirus genus contains several arthropod-borne viruses that pose global health threats, including dengue viruses (denv), yellow fever virus (yfv), and zika virus (zikv). in order to understand how these viruses replicate in human cells, we previously conducted genome-scale rna interference screens to identify candidate host factors. in these screens, we identified ribosomal proteins rplp1 and rplp2 (rplp1/2) to be among the most crucial putative host factors required for denv and yfv infe ... | 2017 | 27974556 |
| diversity of yellow fever mosquito vectors in the atlantic forest of rio de janeiro, brazil. | environmental modifications caused by human activities have led to changes in mosquito vector populations, and sylvatic species have adapted to breeding in urban areas. | 2017 | 27384833 |
| spatial quantification of the world population potentially exposed to zika virus. | zika virus is an emerging flaviviridae virus, which has spread rapidly in the last few years. it has raised concern because it has been associated with fetus microcephaly when pregnant women are infected. the main vector is the mosquito aedes aegypti , distributed in tropical areas. | 2017 | 28338754 |
| boswellia ovalifoliolata (burseraceae) essential oil as an eco-friendly larvicide? toxicity against six mosquito vectors of public health importance, non-target mosquito fishes, backswimmers, and water bugs. | the use of synthetic pesticides to control vector populations is detrimental to human health and the environment and may lead to the development of resistant strains. plants can be alternative sources of safer compounds effective on mosquito vectors. in this study, the mosquito larvicidal activity of boswellia ovalifoliolata leaf essential oil (eo) was evaluated against anopheles stephensi, anopheles subpictus, aedes aegypti, aedes albopictus, culex quinquefasciatus, and culex tritaeniorhynchus. ... | 2017 | 28332087 |
| ascension island: a survey to assess the presence of zika virus vectors. | following concerns regarding the spread of zika virus, joint services health unit (cyprus) were tasked to carry out a mosquito survey on the ascension island, south atlantic. this was to determine if vectors of the virus such as aedes aegypti and aedes albopictus were introduced and established on the island. | 2017 | 28331047 |
| spatial-temporal analysis of dengue deaths: identifying social vulnerabilities. | currently, dengue fever, chikungunya fever, and zika virus represent serious public health issues in brazil, despite efforts to control the vector, the aedes aegypti mosquito. | 2017 | 28327810 |
| correction: laboratory strains of aedes aegypti are competent to brazilian zika virus. | [this corrects the article doi: 10.1371/journal.pone.0171951.]. | 2017 | 28288192 |
| variation in aedes aegypti mosquito competence for zika virus transmission. | to test whether zika virus has adapted for more efficient transmission by aedes aegypti mosquitoes, leading to recent urban outbreaks, we fed mosquitoes from brazil, the dominican republic, and the united states artificial blood meals containing 1 of 3 zika virus strains (senegal, cambodia, mexico) and monitored infection, dissemination, and virus in saliva. contrary to our hypothesis, cambodia and mexica strains were less infectious than the senegal strain. only mosquitoes from the dominican re ... | 2017 | 28287375 |
| <i>aedes aegypti</i> control through modernized, integrated vector management. | in the context of the ongoing, unprecedented zika virus outbreak in the americas, the world health organization has expressed its support for developing and up-scaling three novel approaches to controlling the aedes aegypti mosquito: the sterile insect technique (sit), the release of insects carrying dominant lethal genes (ridl) and the release of wolbachia-infected mosquitoes. whereas the former two approaches are temporary insect population suppression strategies, wolbachia infection is a self ... | 2017 | 28286698 |
| vertical transmission of zika virus by aedes aegypti and ae. albopictus mosquitoes. | to determine the potential role of vertical transmission in zika virus expansion, we evaluated larval pools of perorally infected aedes. aegypti and ae. albopictus adult female mosquitoes; ≈1/84 larvae tested were zika virus-positive; and rates varied among mosquito populations. thus, vertical transmission may play a role in zika virus spread and maintenance. | 2017 | 28277199 |
| zika in rio de janeiro: assessment of basic reproduction number and comparison with dengue outbreaks. | zika virus infection was declared a public health emergency of international concern in february 2016 in response to the outbreak in brazil and its suspected link with congenital anomalies. in this study, we use notification data and disease natural history parameters to estimate the basic reproduction number (r 0) of zika in rio de janeiro, brazil. we also obtain estimates of r 0 of dengue from time series of dengue cases in the outbreaks registered in 2002 and 2012 in the city, when denv-3 and ... | 2017 | 28240195 |
| combining contact tracing with targeted indoor residual spraying significantly reduces dengue transmission. | the widespread transmission of dengue viruses (denv), coupled with the alarming increase of birth defects and neurological disorders associated with zika virus, has put the world in dire need of more efficacious tools for aedes aegypti-borne disease mitigation. we quantitatively investigated the epidemiological value of location-based contact tracing (identifying potential out-of-home exposure locations by phone interviews) to infer transmission foci where high-quality insecticide applications c ... | 2017 | 28232955 |
| leaking containers: success and failure in controlling the mosquito aedes aegypti in brazil. | in 1958, the pan american health organization declared that brazil had successfully eradicated the mosquito aedes aegypti, responsible for the transmission of yellow fever, dengue fever, chikungunya, and zika virus. yet in 2016 the brazilian minister of health described the situation of dengue fever as "catastrophic." discussing the recent epidemic of zika virus, which amplified the crisis produced by the persistence of dengue fever, brazil's president declared in january 2016 that "we are in th ... | 2017 | 28207332 |
| does zika virus infection affect mosquito response to repellents? | the world health organization (who) recommends that people travelling to or living in areas with zika virus (zikv) outbreaks or epidemics adopt prophylactic measures to reduce or eliminate mosquito bites, including the use of insect repellents. it is, however, unknown whether repellents are effective against zikv-infected mosquitoes, in part because of the ethical concerns related to exposing a human subject's arm to infected mosquitoes in the standard arm-in-cage assay. we used a previously dev ... | 2017 | 28205633 |
| a cdna clone-launched platform for high-yield production of inactivated zika vaccine. | a purified inactivated vaccine (piv) using the zika virus (zikv) puerto rico strain prvabc59 showed efficacy in monkeys, and is currently in a phase i clinical trial. high-yield manufacture of this piv is essential for its development and vaccine access. here we report an infectious cdna clone-launched platform to maximize its yield. a single ns1 protein substitution (k265e) was identified to increase zikv replication on vero cells (a cell line approved for vaccine production) for both cambodian ... | 2017 | 28196656 |
| laboratory strains of aedes aegypti are competent to brazilian zika virus. | the zika virus outbreaks are unprecedented human threat in relation to congenital malformations and neurological/autoimmune complications. since this virus has high potential to spread in regions presenting the vectors, improvement in mosquito control is a top priority. thus, aedes aegypti laboratory strains will be fundamental to support studies in different research fields implicated on zika-mosquito interactions which are the basis for the development of innovative control methods. in this se ... | 2017 | 28187183 |
| predicting the potential role of non-human hosts in zika virus maintenance. | arboviruses are often maintained in complex cycles involving vertebrates such as mammals or birds and blood-feeding mosquitoes. however, the role of wildlife hosts in their emergence or re-emergence in human populations has received little attention. the recent emergence of zika virus in america, and previous occurrences of chikungunya and dengue, forces us to confront a potential new disease-emergence phenomenon. using a spatial data mining framework to identify potential biotic interactions, b ... | 2017 | 28180996 |
| larvicidal activity of blumea eriantha essential oil and its components against six mosquito species, including zika virus vectors: the promising potential of (4e,6z)-allo-ocimene, carvotanacetone and dodecyl acetate. | the effective and environmentally sustainable control of mosquitoes is a challenge of essential importance. this is due to the fact that some invasive mosquitoes, with special reference to the aedes genus, are particularly difficult to control, due to their high ecological plasticity. moreover, the indiscriminate overuse of synthetic insecticides resulted in undesirable effects on human health and non-target organisms, as well as resistance development in targeted vectors. here, the leaf essenti ... | 2017 | 28168560 |
| development and characterization of recombinant virus generated from a new world zika virus infectious clone. | zika virus (zikv; family flaviviridae, genus flavivirus) is a rapidly expanding global pathogen that has been associated with severe clinical manifestations, including devastating neurological disease in infants. there are currently no molecular clones of a new world zikv available that lack significant attenuation, hindering progress toward understanding determinants of transmission and pathogenesis. here we report the development and characterization of a novel zikv reverse genetics system bas ... | 2017 | 27795432 |
| [zika virus infection in pregnancy]. | the zika virus is a flavivirus spread by mosquitoes. its primary vectors are the aedes aegypti and the aedes albopictus. before 2007 it sporadically caused benign morbidity. since 2015, it started spreading "explosively" in america, especially in brazil. in august 2016 they reported cases from new york and poland, too. most of the infections don't produce any symptoms, but can cause grave complications. the most important lesion is microcephalia that forms in fetuses. microcephalia's most seriou ... | 2017 | 28393598 |
| new records of aedes aegypti in southern oklahoma, 2016. | aedes aegypti is an important subtropical vector species and is predicted to have a limited year-round distribution in the southern united states. collection of the species has not been officially verified in oklahoma since 1940. adult mosquitoes were collected in 42 sites across 7 different cities in oklahoma using 3 different mosquito traps between may and september 2016. between july and september 2016, 88 ae. aegypti adults were collected at 18 different sites in 4 different cities across so ... | 2017 | 28388329 |
| does zika virus cause microcephaly - applying the bradford hill viewpoints. | zika virus has been documented since 1952, but been associated with mild, self-limiting disease. zika virus is classified as an arbovirus from a family of flaviviridae and primarily spread by aedes aegypti mosquitos. however, in a large outbreak in brazil in 2015, zika virus has been associated with microcephaly. | 2017 | 28357156 |
| host-virus interaction of zika virus in modulating disease pathogenesis. | the zika virus (zikv) is a newly emerging pathogen that has resulted in a worldwide epidemic. it primarily spreads either through infected aedes aegypti or aedes albopictus mosquitos leading to severe neurological disorders such as microcephaly and guillain-barré syndrome in susceptible individuals. the mode of zikv entry into specific cell types such as: epidermal keratinocytes, fibroblasts, immature dendritic cells (idcs), and stem-cell-derived human neural progenitors has been determined thro ... | 2017 | 28349242 |
| an integrated approach for the assessment of the aedes aegypti and aedes albopictus global spatial distribution, and determination of the zones susceptible to the development of zika virus. | the zika virus, one of the new epidemic diseases, is reported to have affected millions of people in the past year. the suitable climate conditions of the areas where zika virus has been reported, especially in areas with a high population density, are the main cause of the current outbreak and spread of the disease. indeed, the suitable climatic conditions of certain territories constitute perfect breading nest for the propagation and outbreak of worldwide diseases. the main objective of this r ... | 2017 | 28111132 |
| experimental transmission of zika virus by mosquitoes from central europe. | mosquitoes collected in germany in 2016, including culex pipiens pipiens biotype pipiens, culex torrentium and aedes albopictus, as well as culex pipiens pipiens biotype molestus (in colony since 2011) were experimentally infected with zika virus (zikv) at 18 °c or 27 °c. none of the culex taxa showed vector competence for zikv. in contrast, aedes albopictus were susceptible for zikv but only at 27 °c, with transmission rates similar to an aedes aegypti laboratory colony tested in parallel. | 2017 | 28106528 |
| zika virus vector competency of mosquitoes, gulf coast, united states. | zika virus has recently spread throughout the americas. although aedes aegypti mosquitoes are considered the primary vector, culex quinquefasciatus and mosquitoes of other species may also be vectors. we tested cx. quinquefasciatus and ae. taeniorhynchus mosquitoes from the us gulf coast; both were refractory to infection and incapable of transmission. | 2017 | 28005002 |
| zika virus in asia. | zika virus (zikv) is an emerging mosquito-borne virus that was first isolated from a sentinel rhesus monkey in the zika forest in uganda in 1947. in asia, the virus was isolated in malaysia from aedes aegypti mosquitoes in 1966, and the first human infections were reported in 1977 in central java, indonesia. in this review, all reported cases of zikv infection in asia as of september 1, 2016 are summarized and some of the hypotheses that could currently explain the apparently low incidence of zi ... | 2017 | 27939768 |
| flavivirus transmission focusing on zika. | flaviviruses are among the most diverse viruses with over 85 species recognized. taxonomically, this genus is one of the 4 recognized genera within the family flaviviridae. most flaviviruses of human public health significance, for example, dengue, yellow fever and zika viruses, are arthropod-borne (arboviruses) and have two evolutionarily and ecologically distinct transmission cycles: a sylvatic transmission cycle, where the virus circulates between zoonotic vertebrate reservoir and amplificati ... | 2017 | 27936448 |
| low circulation of zika virus, cambodia, 2007-2016. | we describe a retrospective study on circulation of zika virus in cambodia during 2007-2016 among patients with dengue-like symptoms and aedes aegypti mosquitoes. our findings suggest that zika virus in cambodia belongs to the asia genotype, is endemic, has low prevalence, and has had low-level impact on public health. | 2017 | 27875110 |
| sarniensine, a mesembrine-type alkaloid isolated from nerine sarniensis, an indigenous south african amaryllidaceae, with larvicidal and adulticidal activities against aedes aegypti. | a new mesembrine-type alkaloid, named sarniensine, was isolated together with tazettine, lycorine, the main alkaloid, and 3-epimacronine from nerine sarniensis, with the last two produced for the first time by this plant. this amaryllidaceae, which is indigenous of south africa, was investigated for its alkaloid content, because the organic extract of its bulbs showed strong larvicidal activity with an lc50 value of 0.008μgμl(-1) against first instar aedes aegypti larvae and with an ld50 value 4 ... | 2017 | 27864138 |
| understanding zika virus. | this article describes what pediatric healthcare professionals should know about zika virus (zikv). | 2017 | 27862959 |
| toxicity of ar-curcumene and epi-β-bisabolol from hedychium larsenii (zingiberaceae) essential oil on malaria, chikungunya and st. louis encephalitis mosquito vectors. | mosquitoes act as vectors of key pathogens and parasites. plant essential oils have been recognized as important sources of biopesticides, which do not induce resistance and have limited toxic effects on human health and non-target organisms. in this research, we evaluated the larvicidal and oviposition deterrence activity of hedychium larsenii essential oil (eo) and its major compounds ar-curcumene and epi-β-bisabolol. both molecules showed high toxicity against early third instars of anopheles ... | 2017 | 27918946 |
| zika virus-what the otolaryngologist should know: a review. | initially discovered in 1947, zika virus infection received little notoriety as a tropical disease until 2015 when an outbreak of microcephaly cases was reported in brazil. zika is a single-stranded rna arbovirus of the flaviviridae family. the primary source of infection in humans stems from aedes aegypti mosquito bites but can also occur through sexual, blood, and perinatal transmission. with expectations that 3 to 4 million people across the americas will be infected over the next year, the w ... | 2017 | 27892990 |
| duplex real-time pcr assay distinguishes aedes aegypti from ae. albopictus (diptera: culicidae) using dna from sonicated first-instar larvae. | aedes aegypti (l.) and ae. albopictus (skuse) are important arbovirus vectors in the united states, and the recent emergence of zika virus disease as a public health concern in the americas has reinforced a need for tools to rapidly distinguish between these species in collections made by vector control agencies. we developed a duplex real-time pcr assay that detects both species and does not cross-amplify in any of the other seven aedes species tested. the lower limit of detection for our assay ... | 2017 | 28981691 |
| pyrethroid susceptibility has been maintained in the dengue vector, aedes aegypti (diptera: culicidae), in queensland, australia. | although pesticide resistance is common in insect vectors of human diseases, the evolution of resistance might be delayed if management practices are adopted that limit selection of resistance alleles. outbreaks of dengue fever have occurred in queensland, australia, since the late 1800s, leading to ongoing attempts to control the mosquito vector, aedes aegypti (l.). since the 1990s, pyrethroid insecticides have been used for this purpose, but have been applied in a strategic manner with a varie ... | 2017 | 28981684 |
| resistance status and resistance mechanisms in a strain of aedes aegypti (diptera: culicidae) from puerto rico. | puerto rico (pr) has a long history of vector-borne disease and insecticide-resistant aedes aegypti (l.). defining contributing mechanisms behind phenotypic resistance is critical for effective vector control intervention. however, previous studies from pr have each focused on only one mechanism of pyrethroid resistance. this study examines the contribution of p450-mediated enzymatic detoxification and sodium channel target site changes to the overall resistance phenotype of ae. aegypti collecte ... | 2017 | 28981681 |
| cutaneous manifestations of chikungunya fever: observations from an outbreak at a tertiary care hospital in southeast rajasthan, india. | chikungunya fever is caused by chikungunya virus which is transmitted by the bite of infected aedes aegypti and a. albopictus mosquitoes. | 2017 | 28979866 |
| infection rate of aedes aegypti mosquitoes with dengue virus depends on the interaction between temperature and mosquito genotype. | dengue fever is the most prevalent arthropod-transmitted viral disease worldwide, with endemic transmission restricted to tropical and subtropical regions of different temperature profiles. temperature is epidemiologically relevant because it affects dengue infection rates in aedes aegypti mosquitoes, the major vector of the dengue virus (denv). aedes aegypti populations are also known to vary in competence for different denv genotypes. we assessed the effects of mosquito and virus genotype on d ... | 2017 | 28978730 |
| identification of surveillance and control priority areas for dengue and other arboviruses transmitted by aedes aegypti in natal-rn, brazil: experience report. | to report the vigi@dengue experience, held in the municipality of natal-rn, brazil, from october/2015 to may/2016. | 2017 | 28977187 |
| circulation of denv2 and denv4 in aedes aegypti (diptera: culicidae) mosquitoes from praia, santiago island, cabo verde. | arthropod-borne viruses, such as dengue (denv), chikungunya (chikv), and zika (zikv), pose a challenge to public health, due to their worldwide distribution and large-scale outbreaks. dengue fever is currently one of the most important diseases and it is caused by four serotypes of denv and is mainly transmitted by the mosquito aedes aegypti. it is estimated that 50-100 million cases are reported every year worldwide. more recently, chikv and zikv, which are also transmitted by ae. aegypti, have ... | 2017 | 28973490 |
| modeling the environmental suitability for aedes (stegomyia) aegypti and aedes (stegomyia) albopictus (diptera: culicidae) in the contiguous united states. | the mosquitoes aedes (stegomyia) aegypti (l.)(diptera:culicidae) and ae. (stegomyia) albopictus (skuse) (diptera:culicidae) transmit dengue, chikungunya, and zika viruses and represent a growing public health threat in parts of the united states where they are established. to complement existing mosquito presence records based on discontinuous, non-systematic surveillance efforts, we developed county-scale environmental suitability maps for both species using maximum entropy modeling to fit clim ... | 2017 | 29029153 |
| combined toxicity of three essential oils against aedes aegypti (diptera: culicidae) larvae. | essential oils are potential alternatives to synthetic insecticides because they have low mammalian toxicity, degrade rapidly in the environment, and possess complex mixtures of bioactive constituents with multi-modal activity against the target insect populations. twenty-one essential oils were initially screened for their toxicity against aedes aegypti (l.) larvae and three out of the seven most toxic essential oils (manuka, oregano, and clove bud essential oils) were examined for their chemic ... | 2017 | 29029151 |
| effect of climatic conditions and water bodies on population dynamics of the dengue vector, aedes aegypti (diptera: culicidae). | the population of mosquitoes is mainly influenced by the biotic and abiotic factors. although aedes aegypti was reported until 1960's in the punjab, pakistan, the population has increased dramatically since 2009 and caused severe epidemics in 2011 due to heavy floods and rains. thus, this study was carried out to know the effect of biotic and abiotic factors on the population of aedes aegypti. | 2017 | 29026852 |
| central nervous system effects of intrauterine zika virus infection: a pictorial review. | relatively few agents have been associated with congenital infections involving the brain. one such agent is the zika virus, which has caused several outbreaks worldwide and has spread in the americas since 2015. the zika virus is an arbovirus transmitted by infected female mosquito vectors, such as the aedes aegypti mosquito. this virus has been commonly associated with congenital infections of the central nervous system and has greatly increased the rates of microcephaly. ultrasonography (us) ... | 2017 | 29019762 |
| a multiplex pcr for detection of knockdown resistance mutations, v1016g and f1534c, in pyrethroid-resistant aedes aegypti. | mutation of the voltage-gated sodium channel (vgsc) gene, or knockdown resistance (kdr) gene, is an important resistance mechanism of the dengue vector aedes aegypti mosquitoes against pyrethroids. in many countries in asia, a valine to glycine substitution (v1016g) and a phenylalanine to cysteine substitution (f1534c) are common in ae. aegypti populations. the g1016 and c1534 allele frequencies have been increasing in recent years, and hence there is a need to have a simple and inexpensive tool ... | 2017 | 29017613 |
| temporal distribution and insecticide resistance profile of two major arbovirus vectors aedes aegypti and aedes albopictus in yaoundé, the capital city of cameroon. | aedes aegypti and ae. albopictus are the major epidemic vectors of several arbovirus diseases such as yellow fever, dengue, zika and chikungunya worldwide. both aedes vectors are presents in cameroon; however, knowledge on the dynamic of the distribution of these species across cities and their resistance profile to insecticide are limited. here, we assessed the current distribution of ae. aegypti and ae. albopictus in yaoundé, the capital city, established the resistance profile to insecticides ... | 2017 | 29017606 |
| hydrogen peroxide resistance in strigomonas culicis: effects on mitochondrial functionality and aedes aegypti interaction. | reactive oxygen species (ros) are toxic molecules involved in several biological processes such as cellular signaling, proliferation, differentiation and cell death. adaptations to oxidative environments are crucial for the success of the colonization of insects by protozoa. strigomonas culicis is a monoxenic trypanosomatid found in the midgut of mosquitoes and presenting a life cycle restricted to the epimastigote form. among s. culicis peculiarities, there is an endosymbiotic bacterium in the ... | 2017 | 28993269 |
| evaluation of gamma radiation-induced dna damage in aedes aegypti using the comet assay. | the study was undertaken to evaluate gamma radiation-induced dna damage in aedes aegypti. the comet assay was employed to demonstrate the extent of dna damage produced in adult male a. aegypti exposed to seven different doses of gamma radiation, ranging from 1 gy to 50 gy. dna damage was measured as the percentage of comet tail dna. a significant linear increase in dna damage was observed in all samples; the extent of damage being proportional to the dose of gamma radiation the organism received ... | 2017 | 28992792 |
| how does competition among wild type mosquitoes influence the performance of aedes aegypti and dissemination of wolbachia pipientis? | wolbachia has been deployed in several countries to reduce transmission of dengue, zika and chikungunya viruses. during releases, wolbachia-infected females are likely to lay their eggs in local available breeding sites, which might already be colonized by local aedes sp. mosquitoes. therefore, there is an urgent need to estimate the deleterious effects of intra and interspecific larval competition on mosquito life history traits, especially on the duration of larval development time, larval mor ... | 2017 | 28991902 |
| characterization of the zika virus induced small rna response in aedes aegypti cells. | rna interference (rnai) controls arbovirus infections in mosquitoes. two different rnai pathways are involved in antiviral responses: the piwi-interacting rna (pirna) and exogenous short interfering rna (exo-sirna) pathways, which are characterized by the production of virus-derived small rnas of 25-29 and 21 nucleotides, respectively. the exo-sirna pathway is considered to be the key mosquito antiviral response mechanism. in aedes aegypti-derived cells, zika virus (zikv)-specific sirnas were pr ... | 2017 | 29040304 |
| patchy dna forms of the zika virus rna genome are generated following infection in mosquito cell cultures and in mosquitoes. | zika virus (zikv) is a mosquito-borne flavivirus and has historically been reported to cause mild symptomatic diseases during human infections. more recently, the explosion of microcephaly among infants born to zikv-infected women has made zikv a global public health concern. while zikv causes acute human diseases, infections of vector mosquitoes are basically non-pathogenic, allowing persistent infections and conferring lifelong ability to transmit the virus. recent studies have revealed that d ... | 2017 | 29039731 |
| lure-and-kill yeast interfering rna larvicides targeting neural genes in the human disease vector mosquito aedes aegypti. | new mosquito control strategies are vitally needed to address established arthropod-borne infectious diseases such as dengue and yellow fever and emerging diseases such as zika and chikungunya, all of which are transmitted by the disease vector mosquito aedes aegypti. in this investigation, saccharomyces cerevisiae (baker's yeast) was engineered to produce short hairpin rnas (shrnas) corresponding to the aedes aegypti orthologs of fasciculation and elongation protein zeta 2 (fez2) and leukocyte ... | 2017 | 29038510 |
| using phage display technology to obtain crybodies active against non-target insects. | the insecticidal cry toxins produced by bacillus thuringiensis (bt) are increasingly important in the biological control of insect pests and vectors of human disease. markets for bt products and transgenic plants expressing their toxins are driven by their specificity, safety and the move away from chemical control agents. however, the high specificity of cry toxins can also prove to be a limitation when there is no known cry toxin active against a particular target. novel activities can be disc ... | 2017 | 29097681 |
| efficiency of two larval diets for mass-rearing of the mosquito aedes aegypti. | aedes aegypti is a major vector of arboviruses that may be controlled on an area-wide basis using the sterile insect technique (sit). larval diet is a major factor in mass-rearing for sit programs. we compared dietary effects on immature development and adult fitness-related characteristics for an international atomic energy agency (iaea) diet, developed for rearing ae. albopictus, and a standardized laboratory rodent diet (lrd), under a 14:10 h (light:dark) photoperiod ("light" treatment) or co ... | 2017 | 29095933 |
| insecticide susceptibility screening against culex and aedes (diptera: culicidae) mosquitoes from the united states. | mosquitoes exposed to sublethal doses of insecticides may be selected for resistance to insecticide active ingredients (ais). mosquitoes are exposed to ais through agricultural, public/private mosquito control programs, homeowners, and other sources. hence, mosquito control programs should routinely measure the resistance/susceptibility status of mosquito populations of public health concern. the objectives here were to determine resistance status for six ais used in adult mosquito control in th ... | 2017 | 29186579 |
| functional analysis of glycosylation of zika virus envelope protein. | zika virus (zikv) infection causes devastating congenital abnormities and guillain-barré syndrome. the zikv envelope (e) protein is responsible for viral entry and represents a major determinant for viral pathogenesis. like other flaviviruses, the zikv e protein is glycosylated at amino acid n154. to study the function of e glycosylation, we generated a recombinant n154q zikv that lacks the e glycosylation and analyzed the mutant virus in mammalian and mosquito hosts. in mouse models, the mutant ... | 2017 | 29091758 |
| darker eggs of mosquitoes resist more to dry conditions: melanin enhances serosal cuticle contribution in egg resistance to desiccation in aedes, anopheles and culex vectors. | mosquito vectors lay their white eggs in the aquatic milieu. during early embryogenesis water passes freely through the transparent eggshell, which at this moment is composed of exochorion and endochorion. within two hours the endochorion darkens via melanization but even so eggs shrink and perish if removed from moisture. however, during mid-embryogenesis, cells of the extraembryonic serosa secrete the serosal cuticle, localized right below the endochorion, becoming the third and innermost eggs ... | 2017 | 29084225 |
| assessing the population at risk of zika virus in asia - is the emergency really over? | on november 18, 2016, the who ended its designation of the zika virus (zikv) epidemic as a public health emergency of international concern (pheic). at the same time, zikv transmission continues in asia, with the number of asian countries reporting zika cases increasing over the last 2 years. applying a method that combines epidemiological theory with data on epidemic size and drivers of transmission, we characterised the population at risk of zikv infection from aedes aegypti mosquitoes in 15 c ... | 2017 | 29082009 |
| culex quinquefasciatus mosquitoes do not support replication of zika virus. | the rapid spread of zika virus (zikv) in the americas raised many questions about the role of culex quinquefasciatus mosquitoes in transmission, in addition to the key role played by the vector aedes aegypti. here we analysed the competence of cx. quinquefasciatus (with or without wolbachia endosymbionts) for a zikv isolate. we also examined the induction of rna interference pathways after viral challenge and the production of small virus-derived rnas. we did not observe any infection nor such s ... | 2017 | 29076805 |
| voltage-sensitive sodium channel mutations s989p + v1016g in aedes aegypti confer variable resistance to pyrethroids, ddt and oxadiazines. | aedes aegypti is a vector of several important human pathogens. control efforts rely primarily on pyrethroid insecticides for adult mosquito control, especially during disease outbreaks. a. aegypti has developed resistance nearly everywhere it occurs and insecticides are used. an important mechanism of resistance is due to mutations in the voltage-sensitive sodium channel (vssc) gene. two mutations, in particular, s989p + v1016g, commonly occur together in parts of asia. | 2017 | 29064635 |
| asymmetrical competition between aedes aegypti and culex quinquefasciatus (diptera: culicidae) coexisting in breeding sites. | aedes aegypti and culex quinquefasciatus are mosquito vectors for several tropical diseases that represent a current public health problem. the ecological requirements for each species are different, however, both species show high biological adaptability, which promotes their coexistence in the same breeding sites. the purpose of this study was to assess the effect of larval association between ae. aegypti and cx. quinquefasciatus under different laboratory conditions of food supply and tempera ... | 2017 | 29064390 |
| assessment of the insecticidal activity of afoxolaner against aedes aegypti in dogs treated with nexgard®. | twelve healthy dogs were studied in this parallel group, blinded, randomised, and negative controlled efficacy study. on day -1, the 12 dogs included were ranked within sex in descending order of individual pre-treatment (day -5) fed mosquito counts and randomly allocated by blocks of two dogs to the untreated control group or the afoxolaner-treated group. nexgard® (merial, now part of boehringer ingelheim animal health) was administered orally on day 0 in accordance with the european label inst ... | 2017 | 29063828 |
| synthesis and chemometrics of thymol and carvacrol derivatives as larvicides against aedes aegypti. | thymol and carvacrol have previously demonstrated larvicidal activity against aedes aegypti (diptera: culicidae). in view of this fact, it was of our interest to obtain synthetic derivatives and evaluate their larvicidal activity on ae. aegypti larvae. | 2017 | 29062856 |
| evaluation of larvicidal activity of essential oil from leaves of coccinia grandis against three mosquito species. | to study the chemical constituents and larvicidal activity of essential oil extracted from the leaves of coccinia grandis against three mosquito species. | 2017 | 29062847 |
| use of a recombinant mosquito densovirus as a gene delivery vector for the functional analysis of genes in mosquito larvae. | in vivo microinjection is the most commonly used gene transfer technique for analyzing the gene functions in individual mosquitoes. however, this method requires a more technically demanding operation and involves complicated procedures, especially when used in larvae due to their small size, relatively thin and fragile cuticle, and high mortality, which limit its application. in contrast, viral vectors for gene delivery have been developed to surmount extracellular and intracellular barriers. t ... | 2017 | 29053694 |
| designing ecofriendly bionanocomposite assembly with improved antimicrobial and potent on-site zika virus vector larvicidal activities with its mode of action. | dialyzed natural polymer, fibroin, from bombyx mori was used to synthesize biocompatible silver and gold nanoparticles in-situ in dispersion form. the films of pure fibroin (pf), fibroin-silver nanocomposite (fsnc) and fibroin-gold nanocomposite (fgnc) were fabricated by drop casting method. the characterization of the resultant dispersion and films was performed by visual color change, uv-vis spectroscopy and atomic force microscopy. the dispersions of pf, fsnc and fgnc were tested for antibact ... | 2017 | 29138496 |
| germline cas9 expression yields highly efficient genome engineering in a major worldwide disease vector, aedes aegypti. | the development of crispr/cas9 technologies has dramatically increased the accessibility and efficiency of genome editing in many organisms. in general, in vivo germline expression of cas9 results in substantially higher activity than embryonic injection. however, no transgenic lines expressing cas9 have been developed for the major mosquito disease vector aedes aegypti here, we describe the generation of multiple stable, transgenic ae. aegypti strains expressing cas9 in the germline, resulting ... | 2017 | 29138316 |
| incidence of dengue and chikungunya viruses in mosquitoes and human patients in border provinces of vietnam. | dengue virus remains a major threat in vietnam, while chikungunya virus is expected to become one. surveillance was conducted from 2012 to 2014 in vietnam to assess the presence of dengue and chikungunya viruses in patients hospitalized with acute fever in five vietnam provinces neighboring lao pdr and cambodia. surveillance was extended to mosquitoes present in the vicinity of the patients' households. | 2017 | 29121985 |
| the impact of temperature and wolbachia infection on vector competence of potential dengue vectors aedes aegypti and aedes albopictus in the transmission of dengue virus serotype 1 in southern taiwan. | we evaluated the impact of temperature and wolbachia infection on vector competence of the local aedes aegypti and ae. albopictus populations of southern taiwan in the laboratory. | 2017 | 29116011 |
| assessment of achyranthes aspera induced toxicity and molecular analysis of rapd-pcr profiles of larval genomic dna of aedes aegypti l. (diptera: culicidae). | current studies investigated the anti-mosquito potential of achyranthes aspera against the dengue vector, aedes aegypti. the stems and leaves of a. aspera were extracted in hexane and evaluated for their toxicity against early fourth instars of a. aegypti. the larvicidal efficacy of the extract was validated as per who protocol. the mortality counts were made after 24 h and lc values were calculated at different levels. the adverse impact of extracts was also explored on the larval genomic dna. ... | 2017 | 29114143 |
| a blood meal enhances innexin mrna expression in the midgut, malpighian tubules, and ovaries of the yellow fever mosquito aedes aegypti. | mosquitoes are vectors of pathogens that cause diseases of medical and veterinary importance. female mosquitoes transmit these pathogens while taking a blood meal, which most species require to produce eggs. the period after a blood meal is a time of extreme physiological change that requires rapid coordination of specific tissues. gap junctions (gj) are intercellular channels that aid in the coordination of cells within tissues via the direct transfer of certain small molecules and ions between ... | 2017 | 29113099 |
| aedes aegypti molecular responses to zika virus: modulation of infection by the toll and jak/stat immune pathways and virus host factors. | zika (zikv) and dengue virus (denv) are transmitted to humans by aedes mosquitoes. however, the molecular interactions between the vector and zikv remain largely unexplored. in this work, we further investigated the tropism of zikv in two different aedes aegypti strains and show that the virus infection kinetics, tissue migration, and susceptibility to infection differ between mosquito strains. we also compare the vector transcriptome changes upon zikv or denv infection demonstrating that 40% of ... | 2017 | 29109710 |
| surveillance of intensity level and geographical spreading of dengue outbreak among males and females in punjab, pakistan: a case study of 2011. | dengue fever is viral disease which spreads due to the bite of the aedes aegypti mosquito. in recent years, it has affected around 40% population of the world. its endemic flow has led to a large disease burden, in terms of human and financial resources. | 2017 | 29103928 |
| the diversity, structure, and function of heritable adaptive immunity sequences in the aedes aegypti genome. | the aedes aegypti mosquito transmits arboviruses, including dengue, chikungunya, and zika virus. understanding the mechanisms underlying mosquito immunity could provide new tools to control arbovirus spread. insects exploit two different rnai pathways to combat viral and transposon infection: short interfering rnas (sirnas) and piwi-interacting rnas (pirnas) [1, 2]. endogenous viral elements (eves) are sequences from non-retroviral viruses that are inserted into the mosquito genome and can act a ... | 2017 | 29129531 |