Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| identification and partial characterization of a dynamin-like protein, ehdlp1, from the protist parasite entamoeba histolytica. | the dynamin superfamily of proteins includes a large repertoire of evolutionarily conserved gtpases that interact with different subcellular organelle membranes in eukaryotes. dynamins are thought to participate in a number of cellular processes involving membrane remodeling and scission. dynamin-like proteins (dlps) form a subfamily of this vast class and play important roles in cellular processes, such as mitochondrial fission, cytokinesis, and endocytosis. in the present study, a gene encodin ... | 2010 | 19915078 |
| ultrastructural changes on entamoeba histolytica hm1-imss caused by the flavan-3-ol, (-)-epicatechin. | the flavan-3-ol, (-)-epicatechin has been previously identified as the most important antiamoebic compound among the extracts from two medicinal plants: rubus coriifolius and geranium mexicanum. here we report the effects of epicatechin on entamoeba histolytica morphology, analyzed by electronic microscopy. e. histolytica trophozoites were incubated for 48 h at 37 degrees c in the presence of 1.9 microg/ml epicatechin and processed for electronic microscopy analysis. epicatechin induced nuclear ... | 2010 | 19918717 |
| characterization of two isotypes of l-threonine dehydratase from entamoeba histolytica. | the genome sequence of the enteric protozoan parasite entamoeba histolytica suggests that amino acid catabolism plays an important role in energy metabolism. in the present study, we described kinetic and regulatory properties of catabolic l-threonine and l-serine dehydratase (td) from e. histolytica. td catalyses the pyridoxal phosphate-dependent dehydrative deamination of l-threonine and l-serine to ammonia and keto acids (2-oxobutyrate and pyruvate, respectively). e. histolytica possesses two ... | 2010 | 19931317 |
| microwave assisted synthesis, characterization and biological evaluation of palladium and platinum complexes with azomethines. | reactions of 3-acetyl-2,5-dimethylthiophene with thiosemicarbazide and semicarbazide hydrochloride resulted in the formation of new heterocyclic ketimines, 3-acetyl-2,5-dimethylthiophene thiosemicarbazone (c(9)h(13)n(3)os(2) or l(1)h) and 3-acetyl-2,5- dimethylthiophene semicarbazone (c(9)h(13)n(3)os or l(2)h), respectively. the pd(ii) and pt(ii) complexes have been synthesized by mixing metal salts in 1:2 molar ratios with these ligands by using microwave as well as conventional heating method ... | 2010 | 19962340 |
| use of thermal melt curves to assess the quality of enzyme preparations. | this study sought to determine whether the quality of enzyme preparations can be determined from their melting curves, which may easily be obtained using a fluorescent probe and a standard reverse transcription-polymerase chain reaction (rt-pcr) machine. thermal melt data on 31 recombinant enzymes from plasmodium parasites were acquired by incrementally heating them to 90 degrees c and measuring unfolding with a fluorescent dye. activity assays specific to each enzyme were also performed. four o ... | 2010 | 20018159 |
| integrating horizontal gene transfer and common descent to depict evolution and contrast it with "common design". | horizontal gene transfer (hgt) and common descent interact in space and time. because events of hgt co-occur with phylogenetic evolution, it is difficult to depict evolutionary patterns graphically. tree-like representations of life's diversification are useful, but they ignore the significance of hgt in evolutionary history, particularly of unicellular organisms, ancestors of multicellular life. here we integrate the reticulated-tree model, ring of life, symbiogenesis whole-organism model, and ... | 2010 | 20021546 |
| phospholipid-binding protein ehc2a mediates calcium-dependent translocation of transcription factor ure3-bp to the plasma membrane of entamoeba histolytica. | the entamoeba histolytica upstream regulatory element 3-binding protein (ure3-bp) is a transcription factor that binds dna in a ca(2+)-inhibitable manner. the protein is located in both the nucleus and the cytoplasm but has also been found to be enriched in the plasma membrane of amebic trophozoites. we investigated the reason for the unusual localization of ure3-bp at the amebic plasma membrane. here we identify and characterize a 22-kda ca(2+)-dependent binding partner of ure3-bp, ehc2a, a nov ... | 2010 | 20023071 |
| trna gene sequences are required for transcriptional silencing in entamoeba histolytica. | transcriptional silencing by trans inactivation can contribute to the regulation of gene expression in eukaryotic cells. in the human intestinal protozoan parasite entamoeba histolytica, trans inactivation of the amoebapore-a gene (ap-a) was recently achieved by episomal transfection of e. histolytica trophozoites with the plasmid psap1. the mechanism of ap-a trans inactivation is largely unknown, though it was suggested that a partial short interspersed transposable element (sine) is required. ... | 2010 | 20023072 |
| entamoeba histolytica: a unicellular organism containing two active genes encoding for members of the tbp family. | entamoeba histolytica is the protozoan parasite which causes human amoebiasis. in this parasite, few encoding genes for transcription factors have been cloned and characterized. the e. histolytica tata-box binding protein (ehtbp) is the first basal transcription factor that has been studied. to continue with the identification of other members of the basal transcription machinery, we performed an in silico analysis of the e. histolytica genome and found three loci encoding for polypeptides with ... | 2010 | 20026212 |
| bacterial-type oxygen detoxification and iron-sulfur cluster assembly in amoebal relict mitochondria. | the assembly of vital reactive iron-sulfur (fe-s) cofactors in eukaryotes is mediated by proteins inherited from the original mitochondrial endosymbiont. uniquely among eukaryotes, however, entamoeba and mastigamoeba lack such mitochondrial-type fe-s cluster assembly proteins and possess instead an analogous bacterial-type system acquired by lateral gene transfer. here we demonstrate, using immunomicroscopy and biochemical methods, that beyond their predicted cytosolic distribution the bacterial ... | 2010 | 19888992 |
| epithelial induction of porcine suppressor of cytokine signaling 2 (socs2) gene expression in response to entamoeba histolytica. | suppressor of cytokine signaling (socs) proteins are key physiological regulators of both innate and adaptive immunity. these proteins belong to the three major classes of modulators of cytokines signaling. in the following article, we used porcine polarized intestinal cells to study early response to the protozoan, entamoeba histolytica, and we identified by rapid amplification of cdna ends (race) pcr porcine socs1, socs4, socs5 and socs6 encoding sequences. with more than 92% identity predicte ... | 2010 | 20045027 |
| entamoeba histolytica: identification and partial characterization of alpha-mannosidase activity. | despite their well recognized importance in pathogenesis of entamoeba histolytica there are few studies dealing with the assembly and secretion of glycoproteins that participate in the adhesion to target cells and in the dissemination of the parasite in infected tissues. some of these studies refer to the identification and, in some cases, the characterization of glycosyl transferases and glycosidases involved in the biosynthesis of these macromolecules as well as to compartments involved in the ... | 2010 | 20045693 |
| application of real-time polymerase chain reaction in detection of entamoeba histolytica in pus aspirates of liver abscess patients. | entamoeba histolytica is the second major cause of liver abscess disease in humans, particularly in developing countries. recently, dna molecular-based methods have been employed to enhance the detection of e. histolytica in either pus or stool specimens. in this study, the results of real-time polymerase chain reaction (pcr) to detect e. histolytica dna in pus from liver abscess cases were compared with those of indirect hemagglutination assay on the corresponding serum samples. bacterial cultu ... | 2010 | 20132028 |
| entamoeba histolytica: expression and localization of gal/galnac lectin in virulent and non-virulent variants from hm1:imss strain. | we have purified gal/galnac lectin from entamoeba histolytica by electroelution. the purified protein was used to immunize rabbits and obtain polyclonal igg's anti-lectin. these antibodies were used as tools to analyze the expression and localization of the amoebic lectin in both virulent (veh) and non-virulent (nveh) variants of axenically cultured hm1:imss strain. veh is able to induce liver abscesses in hamsters, whereas nveh has lost this ability. in vitro, amoebic trophozoites from both var ... | 2010 | 20138867 |
| entamoeba histolytica: molecular cloning and characterization of a novel neutral sphingomyelinase. | a novel neutral sphingomyelinase (nsmase) was characterized in entamoeba histolytica trophozoites. smase, a sphingomyelin-specific form of phospholipase c, catalyzes the hydrolysis of sphingomyelin to ceramide and phosphorylcholine. three amebic putative nsmase genes were found to be actively transcribed. mg(2+)-independent nsmase activity in the soluble fraction of the trophozoites was stimulated by mn(2+) and partially inhibited by zn(2+). nsmase activity of the recombinant protein ehnsm1, inc ... | 2010 | 20138872 |
| effect of the monocyte locomotion inhibitory factor (mlif) produced by e. histolityca on cytokines and chemokine receptors in t cd4+ lymphocytes. | entamoeba histolytica produces monocyte locomotion inhibitory factor (mlif), which may contribute to the delayed inflammation observed in amoebic hepatic abscesses. leukocytes are affected through the modulation of cytokine expression and/or production. we evaluated the effects of mlif on the activation and production of intracellular cytokines in human cd4+ t lymphocytes by flow cytometry. cells were stimulated for 24 h with pma, mlif, or pma+mlif. cellular activation was measured using anti-cd ... | 2010 | 20140297 |
| microbicidal effect of the lactoferrin peptides lactoferricin17-30, lactoferrampin265-284, and lactoferrin chimera on the parasite entamoeba histolytica. | entamoeba histolytica is a parasitic protozoan that produces amoebiasis, an intestinal disease characterized by ulcerative colitis and dysentery. in some cases, trophozoites can travel to the liver leading to hepatic abscesses and death. recently, lactoferrin and lactoferricin b have been shown to be amoebicidal in axenic cultures. the aim of this work was to determine whether the lactoferrin-peptides lactoferricin amino acids 17-30, lactoferrampin amino acids 265-284, and lactoferrin chimera wh ... | 2010 | 20140481 |
| intestinal protozoa infections among patients with ulcerative colitis: prevalence and impact on clinical disease course. | epidemiological and microbiologic studies suggest that enteropathogenic microorganisms play a substantial role in the clinical initiation and relapses of inflammatory bowel disease. | 2010 | 20145404 |
| the role of lipopeptidophosphoglycan in the immune response to entamoeba histolytica. | the sensing of pathogen associated molecular patterns (pamps) by innate immune receptors, such as toll-like receptors (tlrs), is the first step in the inflammatory response to pathogens. entamoeba histolytica, the etiological agent of amebiasis, has a surface molecule with the characteristics of a pamp. this molecule, which was termed lipopeptidophosphoglycan (lppg), is recognized through tlr2 and tlr4 and leads to the release of cytokines from human monocytes, macrophages, and dendritic cells; ... | 2010 | 20145703 |
| amoebiasis cutis: clinical suspicion is the key to early diagnosis. | amoebiasis cutis is a rare manifestation of entamoeba histolytica, primarily an intestinal pathogen, which occurs as a complication of amoebic dysentery. primary cutaneous amoebiasis occurs from contamination of pre-existing wounds. a high degree of clinical suspicion and demonstration of trophozoites from lesions are important for making an early diagnosis lest these patients should suffer significant morbidity. a hiv-negative and otherwise healthy 40-year-old man presented with a well-defined, ... | 2010 | 20148845 |
| entamoeba histolytica sirtuin ehsir2a deacetylates tubulin and regulates the number of microtubular assemblies during the cell cycle. | we have discovered four sirtuin genes in entamoeba histolytica, two of which are similar to eukaryotic sirtuins and two to bacterial and archaeal sirtuins. the eukaryotic sirtuin homologue, ehsir2a, showed nad(+)-dependent deacetylase activity and was sensitive to class iii hdac inhibitors. localization of ehsir2a at different cellular sites suggested that this deacetylase could have multiple targets. using an e. histolytica cdna library in the yeast two-hybrid genetic screen, we identified seve ... | 2010 | 20148900 |
| u12 type introns were lost at multiple occasions during evolution. | two categories of introns are known, a common u2 type and a rare u12 type. these two types of introns are removed by distinct spliceosomes. the phylogenetic distribution of spliceosomal rnas that are characteristic of the u12 spliceosome, i.e. the u11, u12, u4atac and u6atac rnas, suggest that u12 spliceosomes were lost in many phylogenetic groups. we have now examined the distribution of u2 and u12 introns in many of these groups. | 2010 | 20149226 |
| seroprevalence of entamoeba histolytica in the context of hiv and aids: the case of vhembe district, in south africa's limpopo province. | in a recent study in northern south africa, the seroprevalence of entamoeba histolytica infection among 257 hiv-positive and 117 hiv-negative individuals was determined, using an elisa for the detection of antibodies reacting with the parasite's galactose/-acetyl-d-galactosamine(gal/galnac)-inhibitable adherence lectin. overall, 34.0% of the 374 participants (36.1% of the females and 28.1% of the males) were found seropositive for e. histolytica. although all age-groups were affected by the amoe ... | 2010 | 20149292 |
| the r2r3 myb protein family in entamoeba histolytica. | the myb dna-binding domain is conserved in vertebrates, plants, and fungi. this domain mediates the dna-binding activity of proteins (that have transcription factor activity) in a sequence-specific manner and is also used for the protection of telomeric regions. the myb dna-binding domain contains three imperfect conserved repeats of 52 amino acids (r1, r2, and r3). within each repeat, there are three tryptophans that are separated by 18 or 19 amino acids. in order to understand the role of myb ... | 2010 | 20156532 |
| in vitro induction of entamoeba histolytica cyst-like structures from trophozoites. | inhibition of encystment can be conceived as a potentially useful mechanism to block the transmission of entamoeba histolytica under natural conditions. unfortunately, amoeba encystment has not been achieved in vitro and drugs inhibiting the formation of cysts are not available. luminal conditions inducing encystment in vivo are also unknown, but cellular stress such as exposure to reactive oxygen species from immune cells or intestinal microbiota could be involved. a role for certain divalent c ... | 2010 | 20169067 |
| differential identification of entamoeba spp. based on the analysis of 18s rrna. | entamoeba histolytica is known to cause intestinal and extra-intestinal disease while the other entamoeba species are not considered to be pathogenic. however, all entamoeba spp. should be reported when identified in clinical samples. entamoeba polecki, entamoeba coli, and entamoeba hartmanii can be differentiated morphologically from e. histolytica, but some of their diagnostic morphologic features overlap. e. histolytica, entamoeba dispar, and entamoeba moshkovskii are morphologically identica ... | 2010 | 20169364 |
| a new nuclear function of the entamoeba histolytica glycolytic enzyme enolase: the metabolic regulation of cytosine-5 methyltransferase 2 (dnmt2) activity. | cytosine-5 methyltransferases of the dnmt2 family function as dna and trna methyltransferases. insight into the role and biological significance of dnmt2 is greatly hampered by a lack of knowledge about its protein interactions. in this report, we address the subject of protein interaction by identifying enolase through a yeast two-hybrid screen as a dnmt2-binding protein. enolase, which is known to catalyze the conversion of 2-phosphoglycerate (2-pg) to phosphoenolpyruvate (pep), was shown to h ... | 2010 | 20174608 |
| dyspnea and dysentery: a case report of pleuropulmonary amebiasis. | pleuropulmonary amebiasis is an uncommon complication of entamoeba histolytica infection. it typically occurs in endemic regions including central and south america, africa and the indian subcontinent. the case of a previously healthy us army male stationed in liberia with an acute onset of fevers, abdominal pain and bloody diarrhea is presented. he developed a productive cough with pleuritic chest pain and imaging revealed multiple liver abscesses, with rupture through the diaphragm causing a l ... | 2010 | 20175253 |
| entamoeba histolytica phosphoserine aminotransferase (ehpsat): insights into the structure-function relationship. | abstract: | 2010 | 20199659 |
| epizootic fatal amebiasis in an outdoor group of old world monkeys. | entamoeba (e.) histolytica is an obligate parasite of humans and non-human primates. | 2010 | 20202078 |
| regulation of gene expression in protozoa parasites. | infections with protozoa parasites are associated with high burdens of morbidity and mortality across the developing world. despite extensive efforts to control the transmission of these parasites, the spread of populations resistant to drugs and the lack of effective vaccines against them contribute to their persistence as major public health problems. parasites should perform a strict control on the expression of genes involved in their pathogenicity, differentiation, immune evasion, or drug r ... | 2010 | 20204171 |
| [overdiagnosis of amebiasis in children with dysentery]. | there are morphologically identical amebaes, but with differences that can distinguish them; one as pathogenic: entamoeba histolytica, and the other: entamoeba dispar, as inoffensive. that brought the new hypothesis that many of the cases treated as amebiasis, weren't so. | 2009 | 20049395 |
| illness in long-term travelers visiting geosentinel clinics. | length of travel appears to be associated with health risks. geosentinel surveillance network data for 4,039 long-term travelers (trip duration >6 months) seen after travel during june 1, 1996, through december 31, 2008, were compared with data for 24,807 short-term travelers (trip duration <1 month). long-term travelers traveled more often than short-term travelers for volunteer activities (39.7% vs. 7.0%) and business (25.2% vs. 13.8%). more long-term travelers were men (57.2% vs. 50.1%) and e ... | 2009 | 19891865 |
| use of bacterially expressed dsrna to downregulate entamoeba histolytica gene expression. | modern rna interference (rnai) methodologies using small interfering rna (sirna) oligonucleotide duplexes or episomally synthesized hairpin rna are valuable tools for the analysis of gene function in the protozoan parasite entamoeba histolytica. however, these approaches still require time-consuming procedures including transfection and drug selection, or costly synthetic molecules. | 2009 | 20037645 |
| mitosomes in entamoeba histolytica contain a sulfate activation pathway. | hydrogenosomes and mitosomes are mitochondrion-related organelles in anaerobic/microaerophilic eukaryotes with highly reduced and divergent functions. the full diversity of their content and function, however, has not been fully determined. to understand the central role of mitosomes in entamoeba histolytica, a parasitic protozoon that causes amoebic dysentery and liver abscesses, we examined the proteomic profile of purified mitosomes. using 2 discontinuous percoll gradient centrifugation and m ... | 2009 | 19995967 |
| fulminant colitis and toxic myocarditis: a unifying cause? | 2009 | 20006131 | |
| an ex-vivo human intestinal model to study entamoeba histolytica pathogenesis. | amoebiasis (a human intestinal infection affecting 50 million people every year) is caused by the protozoan parasite entamoeba histolytica. to study the molecular mechanisms underlying human colon invasion by e. histolytica, we have set up an ex vivo human colon model to study the early steps in amoebiasis. using scanning electron microscopy and histological analyses, we have established that e. histolytica caused the removal of the protective mucus coat during the first two hours of incubation, ... | 2009 | 19936071 |
| association of malnutrition with amebiasis. | observation of a cohort of preschool children in dhaka, bangladesh, is beginning to reveal the contributions of environment, host, and parasite to amebiasis. reviewed here are the associations and interactions of malnutrition, iga and interferon-gamma, human leukocyte antigen alleles, and parasite genotypes to the outcome of infection. future efforts aimed at understanding the mechanisms of these effects are described. | 2009 | 19906225 |
| characterization of the restriction enzyme-like endonuclease encoded by the entamoeba histolytica non-long terminal repeat retrotransposon ehline1. | the genome of the human pathogen entamoeba histolytica, a primitive protist, contains non-long terminal repeat retrotransposable elements called ehlines. these encode reverse transcriptase and endonuclease required for retrotransposition. the endonuclease shows sequence similarity with bacterial restriction endonucleases. here we report the salient enzymatic features of one such endonuclease. the kinetics of an ehline1-encoded endonuclease catalyzed reaction, determined under steady-state and si ... | 2009 | 19878305 |
| [pleuro-pulmonary manifestations disclosing hepatic amoebiasis]. | during a mission in ex-yugoslavia between 2001 and 2004, three french soldiers were sent home because of right pneumopathy, right pleurisy after appendicectomy, haemoptysis and liver haematoma, respectively. they previously were stationed in africa and/or south america. the initial diagnosis was quickly modified: pleuropulmonary manifestations of amoebic hepatic abscess in two cases, and pleuropulmonary amoebiasis in the last case. the outcome was favourable with standard anti-amoebic treatment. ... | 2009 | 19878805 |
| serum leptin concentrations in patients with intestinal parasites. | leptin is a protein hormone controlling food intake and energy expenditure. in all infections including parasitic infections there is loss of appetite and anorexia. the aim of the present study was to clarify the relationship between intestinal parasites and serum leptin concentrations in children and adults. forty patients with intestinal parasites and 34 healthy subjects took part in this study. body weight, height and body mass index (bmi) were measured for all patients and controls. patients ... | 2009 | 19851965 |
| [epidemiological investigation on an outbreak of amoebic dysentery in jiangshan city of zhejiang province]. | the investigation was carried out from 15 may to 10 june 2006 among diarrhea patients of two schools and periphery residents in hecun town, jiangshan city. stool samples were examined for entamoeba histolytica. water samples were taken for microbial analysis. 31 cases with e. histolytica were found,with 74.2% (23 cases) of students and preschool children. 9 cases were found in liuyi kindergarten and 8 cases in hecun central primary school with a prevalence of 7.4% and 0.65%, respectively. among ... | 2009 | 19856515 |
| methionine gamma-lyase: the unique reaction mechanism, physiological roles, and therapeutic applications against infectious diseases and cancers. | sulfur-containing amino acids (saas) are essential components in many biological processes and ubiquitously distributed to all organisms. both biosynthetic and catabolic pathways of saas are heterogeneous among organisms and between developmental stages, and regulated by the environmental changes. limited lineage of organisms ranging from archaea to plants, but not human, possess a unique enzyme methionine gamma-lyase (mgl, ec 4.4.1.11) to directly degrade saa to alpha-keto acids, ammonia, and v ... | 2009 | 19859976 |
| genomic dna k-mer spectra: models and modalities. | the empirical frequencies of dna k-mers in whole genome sequences provide an interesting perspective on genomic complexity, and the availability of large segments of genomic sequence from many organisms means that analysis of k-mers with non-trivial lengths is now possible. | 2009 | 19814784 |
| intestinal parasitic infection and associated risk factors, among children presenting at outpatient clinics in manaus, amazonas state, brazil. | in a cross-sectional study, the prevalences of intestinal parasitic infection and the associated risk factors were explored among children aged 0-10 years attending outpatient clinics in manaus, the capital city of the north brazilian state of amazonas. data indicating socio-economic level and demographic factors were collected in interviews with the accompanying parent or guardian of each child. parasitic infections were detected by the microscopical examination of faecal samples, while nutriti ... | 2009 | 19825280 |
| deficient serum mannose-binding lectin levels and mbl2 polymorphisms increase the risk of single and recurrent cryptosporidium infections in young children. | mannose-binding lectin (mbl) is an evolutionarily conserved protein that functions in human innate immunity by binding to microbial surfaces and promoting opsonophagocytosis. mbl has been shown to bind to cryptosporidium sporozoites, and earlier work has suggested that the protective role of mbl may be most important in childhood. we evaluated the association between polymorphisms in the mbl gene (mbl2), serum mbl deficiency, and infection with cryptosporidium, entamoeba histolytica, and giardia ... | 2009 | 19827946 |
| identification of entamoeba histolytica and entamoeba dispar by pcr assay of fecal specimens obtained from thai/myanmar border region. | due to the indistinguishable morphology between entamoeba histolytica (pathogenic) and entamoeba dispar (non pathogenic), pcr-based assays were conducted. based on microscopy, suspected entamoeba cells were detected in 30 out of 455 fecal samples obtained from individuals residing at thai/myanmar border region. the target genes for pcr amplification included genes encoding small subunit rrna (ssu-rrna), chitinase and serine rich entamoeba protein. pcr primers derived from ssu-rrna gene amplified ... | 2009 | 19842426 |
| a rare but fatal case of granulomatous amebic encephalitis with brain abscess: the first case reported from turkey. | the incidence of protozoal and helminthic infestations of the central nervous system (cns) is less than 1%, but these infestations tend to follow a fatal course. they are more common among children, the elderly and immunocompromised individuals. cns infections due to entamoeba histolytica have been known for a long time. in recent years, especially in developing countries, there has been an increase in cns infections due to free-living amebas (flas). acute cns infection due to naegleria fowleri, ... | 2009 | 19621290 |
| prevalence of intestinal parasitosis in a rural population in egypt, and its relation to socio-demographic characteristics. | a cross-sectional study was conducted in a village in menoufia governorate, egypt where the majority of people had individual trenches in the houses for sewage disposal with absence of public sewage system. out of 2292 stool samples 47.8% had at least a single infection. multiple infections occurred in 14.9%. entamoeba histolytica was 20%, e. coli 10%, giardia lamblia 10%, ascaris lumbricoides 27.31%, hymenolepis nana 2.96%, schistosoma mansoni 2.45% and ancylostoma duodenale 2.23%. males were s ... | 2009 | 19621655 |
| [sensibility of entamoeba histolytica trophozoites to ivermectin]. | amebiasis caused by entamoeba histolytica is a problem of public world health. the most frequent clinical presentation are the dysentery and the amebic liver abscess. fifty millions of cases and more than 100.000 deaths for this disease are reported annually worldwide. the life cycle of e. histolytica has two phases: trophozoite and cyst. trophozoites are the causal agent of disease. the effective treatment for the amebiasis includes drugs with serious collateral effects. ivermectin is a macroli ... | 2009 | 19622479 |
| parasitological diagnosis combining an internally controlled real-time pcr assay for the detection of four protozoa in stool samples with a testing algorithm for microscopy. | molecular detection of gastrointestinal protozoa is more sensitive and more specific than microscopy but, to date, has not routinely replaced time-consuming microscopic analysis. two internally controlled real-time pcr assays for the combined detection of entamoeba histolytica, giardia lamblia, cryptosporidium spp. and dientamoeba fragilis in single faecal samples were compared with triple faeces test (tft) microscopy results from 397 patient samples. additionally, an algorithm for complete para ... | 2009 | 19624500 |
| gonadectomy inhibits development of experimental amoebic liver abscess in hamsters through downregulation of the inflammatory immune response. | incidence of amoebic liver abscess (ala) in human males is considerably higher than in females, suggesting a role for sex hormones in this parasite infection. we describe here the effect of hamster gonadectomization on the development of ala. after monitoring the decrease of oestradiol in females and testosterone in males to undetectable levels by elisa and radio immuno assay (ria) in serum, hamsters were intraportally infected with entamoeba histolytica trophozoites and killed 7 days later. ala ... | 2009 | 19646209 |
| entamoeba invadens: cloning and molecular characterization of chitinases. | entamoeba histolytica, the causative agent of amebiasis infects through its cyst form and this transmission may be blocked using encystation specific protein as drug target. in this study, we have characterized the enzyme chitinase which express specifically during encystation. the reptilian parasite entamoeba invadens, used as a model for encystation study contain three chitinases. we report the molecular cloning, over-expression and biochemical characterization of all three e. invadens chitina ... | 2009 | 19646441 |
| comparison of two genetically related entamoeba histolytica cell lines derived from the same isolate with different pathogenic properties. | entamoeba histolytica is known for its extraordinary capacity to destroy human tissues, leading to invasive diseases such as ulcerative colitis or extra-intestinal abscesses. in order to identify the virulence factors of this parasite phenotypes and proteomes of two recently identified genetically related cell lines (a and b), derived from the laboratory e. histolytica isolate hm-1:imss, were compared. both cell lines are indistinguishable on the basis of highly polymorphic tandem repeat dna seq ... | 2009 | 19688750 |
| in vitro activity of pyrvinium pamoate against entamoeba histolytica and giardia intestinalis using radiolabelled thymidine incorporation and an sybr green i-based fluorescence assay. | to assess the in vitro activity of the fda-approved antihelminthic drug pyrvinium pamoate against entamoeba histolytica and giardia intestinalis. | 2009 | 19689977 |
| opportunistic parasitic infections in hiv/aids patients presenting with diarrhoea by the level of immunesuppression. | enteric parasites are major cause of diarrhoea in hiv infected individuals. the present study was undertaken to detect enteric parasites in hiv infected patients with diarrhoea at different levels of immunity. | 2009 | 19700803 |
| new synthesis and antiparasitic activity of model 5-aryl-1-methyl-4-nitroimidazoles. | a number of 5-aryl-1-methyl-4-nitroimidazoles 5a-f have been synthesized in good yields by the suzuki coupling reaction between 5-chloro-1-methyl-4-nitroimidazole (3) and arylboronic acids 4a-f, aided by dichlorobis-(triphenylphosphine)palladium(ii), k(2)co(3, )and tetrabutylammonium bromide in water at 70-80 degrees c. compounds 5a-f were characterized by elemental analysis, nmr and ms spectral data. on the basis of in vitro screening data, 5-(3-chlorophenyl)-1-methyl-4-nitro-1h-imidazole (5f) ... | 2009 | 19701122 |
| presence and diagnosis of amebic infestation in turkish patients with active ulcerative colitis. | it is difficult to definitively diagnose acute amebiasis, particularly when this condition is superimposed on inflammatory bowel disease. our goals in this study were to determine the prevalence of amebiasis in individuals with active ulcerative colitis, and to identify clinical and laboratory parameters that are of value for diagnosing amebiasis in this patient group. | 2009 | 19712863 |
| intestinal parasitic infections among inhabitants of karaj city, tehran province, iran in 2006-2008. | karaj is an area with large influx of refugee people in iran. to increase knowledge about parasitic infections, we carried out this research during 2006-2008. we recorded the stool examination results and some of their personal characteristics. a total of 13,915 human stools were examined, and 649 (4.7%) were positive for intestinal parasites. among them, 13 (0.09%) had worm and 636 (4.6%) had protozoan infections. maximum infections belonged to giardia intestinalis, and 534 (3.8%) samples had t ... | 2009 | 19724700 |
| a survey of intestinal protozoan infections among gastroenteritis patients during a 3-year period (2004-2006) in gyeonggi-do (province), south korea. | the incidence and etiology of parasite-associated gastroenteritis during 2004-2006 in gyeonggi-do (province), south korea was determined by means of antigen detection elisa on 6,071 stool specimens collected from 6 general hospitals. at least 1 parasitic agent was detected in 3.4% (208/6,071) of the stool samples. among these, giardia lamblia was the most numerous (152 cases; 2.5%), followed by entamoeba histolytica (25 cases; 0.4%), cryptosporidium parvum (23 cases; 0.4%), and mixed infections ... | 2009 | 19724707 |
| malnutrition and intestinal parasitic infections in school children of gondar, north west ethiopia. | in developing countries, malnutrition is a considerable health problem with prevalence ranges of 4-46%, with 1-10% severely malnourished. | 2009 | 19743775 |
| enteroparasitosis and their ethnographic relationship to food handlers in a tourist and economic center in paraná, southern brazil. | this study reports on the occurrence of enteroparasites based on data from an ethnographic study of food handlers in the city of cascavel, paraná, brazil. fecal material from 343 food handlers of both sexes, between 14 and 75 years of age, was analyzed using lutz, modified ritchie and ziehl-neelsen techniques. ethnographic relationships were investigated by means of specific questionnaires. positive fecal samples were found for 131 (38.2%) handlers. endolimax nana (67.9%) was the predominant spe ... | 2009 | 19229388 |
| prevalence of multi-gastrointestinal infections with helminth, protozoan and campylobacter spp. in guatemalan children. | the prevalence of multi-infections with helminthes, protozoans and campylobacter spp. in guatemalan children is a reflection of differences in the risk factors related to pathogen transmission. | 2009 | 19759480 |
| diagnosis and treatment of acute or persistent diarrhea. | studies of microbial pathogens and the toxins they produce are important for determining the mechanisms by which they cause disease and spread throughout a population. some bacteria produce secretory enterotoxins (such as cholera toxin or the heat-labile or stable enterotoxins produced by escherichia coli) that invade cells directly. others invade cells or produce cytotoxins (such as those produced by shigella, enteroinvasive e coli, or clostridium difficile) that damage cells or trigger host re ... | 2009 | 19457416 |
| dna characterization of simian entamoeba histolytica-like strains to differentiate them from entamoeba histolytica. | two simian entamoeba histolytica-like strains, ehmfas1 and p19-061405, have been suggested to represent a new species based on genetic characterization. sequence analyses of the hexokinase, glucose phosphate isomerase, and phosphoglucomutase genes supported the previous findings of isoenzyme analyses demonstrating a new zymodeme pattern. phylogenetic studies of 18s rdna, 5.8s rdna, the chaperonin 60 gene, and the pyridine nucleotide transhydrogenase gene showed original clusters of simian e. his ... | 2009 | 19471966 |
| high early mortality in patients with chronic acquired immunodeficiency syndrome diarrhea initiating antiretroviral therapy in haiti: a case-control study. | this case-control study examines whether chronic diarrhea at initiation of antiretroviral therapy (art) affects survival of human immunodefiency virus-infected patients. cases (288) were treatment-naive, non-pregnant, adults with self report of frequent loose stool for > 3 weeks at the time art was initiated. one-third of patients had an enteric pathogen identified including cryptosporidium spp., giardia spp., isospora belli, cyclospora cayetanensis, and entamoeba histolytica. control patients ( ... | 2009 | 19478276 |
| involvement of serine proteases in the excystation and metacystic development of entamoeba invadens. | although the functions of cysteine proteases involved in the pathogenicity and differentiation of entamoeba histolytica have been demonstrated, little is known about the functions of serine proteases. we examined the involvement of serine proteases in amoebic excystation and metacystic development using inhibitors specific for serine proteases. entamoeba invadens ip-1 strain was used as the model of excystation and metacystic development of e. histolytica. four serine protease inhibitors, phenyl ... | 2009 | 19479279 |
| an alternative method for dna extraction and pcr identification of entamoeba histolytica and e. dispar in fecal samples. | since it is known that entamoeba dispar is non-pathogenic and morphologically similar to e. histolytica, there are many targets used in pcr for differentiating these species. however, obtaining high quality dna from fecal samples is fundamental for pcr. most methods are laborious or use kits that make diagnosis expensive. in the present work, a new simple, fast and cheap technique of dna extraction from fecal samples was combined with a pcr for an episomal target in order to identify e. histolyt ... | 2009 | 19486545 |
| [the complicated intestinal amebiasis in emergency surgery]. | 18 patients with complicated forms of intestinal amebiasis were operated on acute appendicitis, liver abscess or total necrotic colitis. appendectomy, abscess drainage and colon resection were performed respectively. there were no postoperative deaths. features of amebic appendicitis and total necrotic amebic colitis are described using clinical cases demonstrations. recommendations for the treatment of these forms of amebiasis are given. | 2009 | 19491760 |
| phosphatidylinositol-phosphates mediate cytoskeletal reorganization during phagocytosis via a unique modular protein consisting of rhogef/dh and fyve domains in the parasitic protozoon entamoeba histolytica. | to understand the roles of phosphoinositides [ptdins] in phagocytosis of parasitic eukaryotes, we examined the interaction of phosphatidylinositol-3-phosphate [ptdins(3)p] and putative ptdins-p-binding proteins during phagocytosis in the enteric protozoan parasite entamoeba histolytica. it was previously shown that phagocytosis in e. histolytica is indispensable for virulence and is inhibited by ptdins 3-kinase inhibitors. we demonstrated by time-lapse live imaging that during the initiation of ... | 2009 | 19496789 |
| periodicity and patterns of entamoeba histolytica and e. dispar infection in hiv+/aids patients in mexico. | in a 12-month longitudinal study, a cohort of mexican hiv+/aids patients was checked several times for entamoeba infection, with the parasites identified, as e. histolytica or e. dispar, using pcr. the polymorphic region of the parasites' chitinase genes was investigated by pcr, with the variation in amplicon sizes being used as a measure of the genetic variation among the isolates. the patients found infected with entamoeba at the start of the study displayed varied patterns of infection cleara ... | 2009 | 19508748 |
| entamoeba histolytica, heterologous expression and in situ immunolocalization of ornithine decarboxylase (ehodc). | previous studies from this laboratory have dealt with the purification and biochemical characterization of ornithine decarboxylase (odc) from entamoeba histolytica. enzyme compartmentalization has been described as a major mechanism in the regulation of polyamine metabolism. however, the subcellular location of odc in the human parasite has remained unresolved. to examine this issue, we cloned the full-length gene (ehodc) encoding for the parasite enzyme, whose open reading frame encodes for a p ... | 2009 | 19520076 |
| high genetic diversity among iranian entamoeba dispar isolates based on the noncoding short tandem repeat locus d-a. | this study has identified and characterized the structure of locus d-a, a noncoding short tandem repeat (str) region, also known as locus 1-2, in iranian entamoeba dispar isolates. this polymorphic locus has been shown to be potentially useful in investigating the molecular epidemiology of entamoeba histolytica and e. dispar. the genetic polymorphisms in locus d-a in 28 isolates of e. dispar from three different geographic regions of iran were distinguished using pcr and sequencing, and the resu ... | 2009 | 19524081 |
| concurrent infections and socioeconomic determinants of geohelminth infection: a community study of schoolchildren in periurban guinea-bissau. | we explored the association between subclinical intestinal helminth infections and other gastrointestinal pathogens in 706 schoolchildren from a poor semirural area while adjusting for socioeconomic risk factors. the study was carried out in two neighbouring areas in the capital of guinea-bissau in west africa. children aged 4-12 years were visited and one child per mother was invited to participate in the study. among the 706 children included in the study, helminths were detected in 44.2%, ent ... | 2009 | 19524992 |
| use and endocytosis of iron-containing proteins by entamoeba histolytica trophozoites. | iron is essential for nearly all organisms; in mammals, it is part of proteins such as haemoglobin, and it is captured by transferrin and lactoferrin. transferrin is present in serum, and lactoferrin is secreted by the mucosa and by neutrophils at infection sites, as a host iron-withholding response, sequestering iron away from invading microorganisms. additionally, all cells contain ferritin, which sequesters iron when its intracellular levels are increased, detoxifying and preventing damage. l ... | 2009 | 19539057 |
| reassessment of the epidemiology of amebiasis: state of the art. | the epidemiology of amebiasis has dramatically changed since the separation of entamoeba histolytica and entamoeba dispar species, and the worldwide prevalence of these species has not been estimated until recently. the most cited data regarding prevalence, morbidity, or mortality due to amebiasis is the 1986 walsh report, in which 100,000 deaths are reported to occur worldwide each year due to medical complications of invasive amebiasis. however, the prevalence values of entamoeba histolytica i ... | 2009 | 19540361 |
| [remarkable evolution of hepatic, pleural and pulmonary amoebiasis: a case report]. | entamoeba histolytica, is a human pathogen which is endemic in tropical areas. the most common extra-intestinal locations for disease after the liver are pleural and pulmonary. although the outcome of pleural and pulmonary amoebiasis is generally favourable, it is important to note that a significant proportion of patients do develop pleural and pulmonary after-effects. we report a case of pleural and pulmonary amoebiasis where the evolution of a massive pleural effusion and 3 associated liver a ... | 2009 | 19543174 |
| [tinidazole: a classical anaerobical drug with multiple potential uses nowadays]. | tinidazole is a 5-nitroimidazole active in vitro against a wide variety of anaerobic bacteria and protozoa. tinidazole is an effective treatment against anaerobic microorganisms based on its pharmacokinetic characteristics (c(max) 51 microg/ml, t(1/2) 12.5 h) and its excellent in vitro activity. its long half-life allows once a day regimens. tinidazole is as effective as metronidazole in the treatment of infections caused by t. vaginalis, giardiasis and amebiasis and bacterial vaginosis, malaria ... | 2009 | 19544102 |
| hospital-based surveillance of enteric parasites in kolkata. | diarrhoea is the second leading cause of illness and death in developing countries and the second commonest cause of death due to infectious diseases among children under five in such countries. parasites, as well as bacterial and viral pathogens, are important causes of diarrhoea. however, parasitic infections are sometimes overlooked, leading after a period of time to an uncertain aetiology. in this paper we report the prevalence of giardia lamblia, entamoeba histolytica and cryptosporidium sp ... | 2009 | 19545355 |
| protection against intestinal amebiasis by a recombinant vaccine is transferable by t cells and mediated by gamma interferon. | we have previously shown that vaccination with purified entamoeba histolytica gal/galnac lectin or recombinant subunits can protect mice from intestinal amebiasis upon intracecal challenge. in this study, we demonstrated with adoptive-transfer experiments that this lectin vaccine protection is mediated by t cells but not serum. the cell-mediated immune (cmi) response was characterized by significant gamma interferon (ifn-gamma), interleukin 12 (il-12), il-2, il-10, and il-17 production. to move ... | 2009 | 19564375 |
| cell-free synthesis and combinatorial selective 15n-labeling of the cytotoxic protein amoebapore a from entamoeba histolytica. | amoebapore a is a pore-forming protein produced by the pathogenic parasite entamoeba histolytica, which causes human amoebic dysentery. the pore-forming activity of amoebapore a is regulated by ph-dependent dimerization, a prerequisite for membrane insertion and pore formation. understanding of these important processes has been hampered by the cytotoxicity of amoebapore a, which prevents the production of this protein in cell-based expression systems. in this study, a protocol for the cell-free ... | 2009 | 19573603 |
| intestinal amoebiasis in children and its effect on nutritional status. | to determine the frequency and clinical presentation of amoebic diarrhea in children and its effect on the nutritional status of the affected children. | 2009 | 19576153 |
| physical, structural, and functional properties of the beta1 integrin-like fibronectin receptor (beta1ehfnr) in entamoeba histolytica. | the presence in entamoeba histolytica of a fibronectin (fn) receptor, which is antigenically related to beta1 integrin-like molecules and shows 99% homology with the intermediate subunit-2 of the gal/galnac-specific lectin has been described. the e. histolytica genome has been sequenced, and its analysis shows no integrin sequences. here we provide further evidence to demonstrate that this molecule behaves as integrin-like in its physical, structural and functional properties. the purified beta1 ... | 2009 | 19577014 |
| evidence for a "wattle and daub" model of the cyst wall of entamoeba. | the cyst wall of entamoeba invadens (ei), a model for the human pathogen entamoeba histolytica, is composed of fibrils of chitin and three chitin-binding lectins called jacob, jessie3, and chitinase. here we show chitin, which was detected with wheat germ agglutinin, is made in secretory vesicles prior to its deposition on the surface of encysting ei. jacob lectins, which have tandemly arrayed chitin-binding domains (cbds), and chitinase, which has an n-terminal cbd, were each made early during ... | 2009 | 19578434 |
| a possible cluster of sexually transmitted entamoeba histolytica: genetic analysis of a highly virulent strain. | transmission of entamoeba histolytica generally occurs by fecal excretion of cysts followed by oral ingestion of contaminated food or water. however, fecal-oral transmission may occur within households and long-term care institutions, and sexual transmission occurs among men who have sex with men. epidemiologically linked clusters of e. histolytica infection are rare in industrialized countries. we report such a sexually linked cluster in canada. | 2009 | 19580413 |
| crude extracts of, and purified compounds from, pterocarpus angolensis, and the essential oil of lippia javanica: their in-vitro cytotoxicities and activities against selected bacteria and entamoeba histolytica. | in a recent study, various extracts of pterocarpus angolensis were prepared and tested against bacteria. the acetone extract was found to be the most active against all the bacteria investigated, with minimum inhibitory concentrations varying from 0.0156 mg/ml against staphylococcus aureus to 2 mg/ml against enterobacter cloacae. seven pure compounds were subsequently isolated from the ethanol extract of p. angolensis. using several chromatographic and spectroscopic methods, the structures of fi ... | 2009 | 19583913 |
| aids-associated parasitic diarrhoea. | since the advent of human immunodeficiency virus infection, with its profound and progressive effect on the cellular immune system, a group of human opportunistic pathogens has come into prominence. opportunistic parasitic infection can cause severe morbidity and mortality. because many of these infections are treatable, an early and accurate diagnosis is important. this can be accomplished by a variety of methods such as direct demonstration of parasites and by serological tests to detect antig ... | 2009 | 19584497 |
| rapid diagnosis of intestinal parasitic protozoa, with a focus on entamoeba histolytica. | entamoeba histolytica is an invasive intestinal pathogenic parasitic protozoan that causes amebiasis. it must be distinguished from entamoeba dispar and e. moshkovskii, nonpathogenic commensal parasites of the human gut lumen that are morphologically identical to e. histolytica. detection of specific e. histolytica antigens in stools is a fast, sensitive technique that should be considered as the method of choice. stool real-time pcr is a highly sensitive and specific technique but its high cost ... | 2009 | 19584941 |
| synthesis, characterization, antiamoebic activity and toxicity of novel bisdioxazole derivatives. | cyclization of benzene-1,4-dicarbaldehyde dioxime 1 with different aromatic aldehydes in inert atmosphere yielded the corresponding new bisdioxazoles 2-11. the structure of 2-11 was elucidated by spectral data. in vitro antiamoebic activity was performed against hm1:imss strain of entamoeba histolytica. the results showed that the compounds 3 (ic(50)=1.22 microm), 4 (ic(50)=1.41 microm), 7 (ic(50)=1.05 microm) and 10 (ic(50)=1.01 microm) exhibited better antiamoebic activity than the standard dr ... | 2009 | 19589625 |
| dna repair mechanisms in eukaryotes: special focus in entamoeba histolytica and related protozoan parasites. | eukaryotic cell viability highly relies on genome stability and dna integrity maintenance. the cellular response to dna damage mainly consists of six biological conserved pathways known as homologous recombination repair (hrr), non-homologous end-joining (nhej), base excision repair (ber), mismatch repair (mmr), nucleotide excision repair (ner), and methyltransferase repair that operate in a concerted way to minimize genetic information loss due to a dna lesion. particularly, protozoan parasites ... | 2009 | 19591963 |
| [antigen detection methods in diagnosis of amebiasis, giardiasis and cryptosporidiosis]. | amebiasis is a significant health problem in developing countries. humans are infected by two morphologically identical species of entamoeba. entamoeba histolytica causes amebic colitis and liver abscess, and entamoeba dispar is noninvasive. giardia intestinalis infection is seen worldwide and in all age groups. but giardiasis is especially prevalent in countries with poor sanitation and unsafe water, where it's responsible for most cases of childhood diarrhea. cryptosporidium parvum, a protozoo ... | 2009 | 19598091 |
| nonpathogenic entamoeba dispar quickly outgrows pathogenic entamoeba histolytica in mixed xenic cultures. | entamoeba histolytica and entamoeba dispar are two microscopically indistinguishable amoebae living in the human colon. the former is a pathogen, whereas the latter is a nonpathogenic commensal. using a model system of in vitro cocultures and pcr detection of the entamoeba species, we found that the nonpathogenic species can rapidly outgrow the pathogen in xenic cultures. | 2009 | 19187490 |
| impact of solar radiation in disinfecting drinking water contaminated with giardia duodenalis and entamoeba histolytica/dispar at a point-of-use water treatment. | to determine the impact of natural sunlight in disinfecting water contaminated with cysts of giardia duodenalis and entamoeba histolytica/dispar using plastic containers. | 2009 | 19191972 |
| education and imaging. gastrointestinal: amebic colitis. | 2009 | 19196401 | |
| recent discoveries in the pathogenesis and immune response toward entamoeba histolytica. | entamoeba histolytica is an enteric dwelling human protozoan parasite that causes the disease amoebiasis, which is endemic in the developing world. over the past four decades, considerable effort has been made to understand the parasite and the disease. improved diagnostics can now differentiate pathogenic e. histolytica from that of the related but nonpathogenic entamoeba dispar, thus minimizing screening errors. classically, the triad of gal-lectin, cysteine proteinases and amoebapores of the ... | 2009 | 19207103 |
| in-vitro cytotoxicity and antimicrobial activities, against clinical isolates of campylobacter species and entamoeba histolytica, of local medicinal plants from the venda region, in south africa. | in the quest for alternative treatments against campylobacter jejuni and entamoeba histolytica, which are both aetiological agents of diarrhoea world-wide, the in-vitro activities against the two pathogens of extracts of 18 south african medicinal plants have recently been assessed. forty extracts from the 18 plant species were prepared and tested against 110 clinical isolates of campylobacter spp. in addition, extracts from eight of the plant species were tested against a standard strain (hm-1: ... | 2009 | 19208300 |
| synthesis and in vitro trichomonicidal, giardicidal and amebicidal activity of n-acetamide(sulfonamide)-2-methyl-4-nitro-1h-imidazoles. | two new series of imidazole derivatives (acetamides: 1-8 and sulfonamides: 9-15) were synthesized using a short synthetic route. compound 1 as well as the intermediate 16g were characterized by x-ray crystallography. imidazole derivatives 1-15 were tested in vitro against three unicellular parasites (giardia intestinalis, trichomonas vaginalis and entamoeba histolytica) in comparison with benznidazole (bzn) and metronidazole. compound 1 [n-benzyl-2-(2-methyl-4-nitro-1h-imidazol-1-yl)acetamide] w ... | 2009 | 19208443 |
| using differential gene expression to study entamoeba histolytica pathogenesis. | the release of the entamoeba histolytica genome has facilitated the development of techniques to survey rapidly and to relate gene expression with biology. the association and potential contribution of differential gene expression to the life cycle and the virulence of this protozoan parasite of humans are reviewed here. | 2009 | 19217826 |
| [diarrhea from the infectologist's point of view]. | gastroenteritis is a nonspecific term for various pathologic states of the gastrointestinal tract. gastroenteritis causing pathogens are the second leading cause of morbidity and mortality worldwide. in the developed countries diarrhea is the most common reason for missing work, while in the developing world, it is a leading cause of death. internationally, the mortality rate is 5-10 million deaths each year. "traveller's diarrhea" is a polyetiologic common health problem of international travel ... | 2009 | 19218145 |
| effects of iron depletion on entamoeba histolytica alcohol dehydrogenase 2 (ehadh2) and trophozoite growth: implications for antiamoebic therapy. | the purpose of this study was to determine the mechanism by which iron chelation affects the trophozoite survival of entamoeba histolytica. fe2+ is a cofactor for e. histolytica alcohol dehydrogenase 2 (ehadh2), an essential bifunctional enzyme [alcohol dehydrogenase (adh) and aldehyde dehydrogenase (aldh)] in the glycolytic pathway of e. histolytica. | 2009 | 19218278 |