Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| solubilization of trichloroacetic acid (tca) precipitated microbial proteins via naoh for two-dimensional electrophoresis. | in preparing intracellular microbial samples for one- or two-dimensional electrophoresis, trichloroacetic acid (tca) precipitation is frequently used to remove interfering compounds. solubilization of tca precipitate typically requires the addition of a number of chaotropes or detergents, in a multistep process, that requires hours to carry out. in this study, a simple, rapid, one-step method to solubilize tca precipitated proteins is presented. precipitated proteins are pretreated with 0.2 m na ... | 2003 | 12643547 |
| cloning and overexpression of beta-n-acetylglucosaminidase encoding gene naga from aspergillus oryzae and enzyme-catalyzed synthesis of human milk oligosaccharide. | we isolated a beta-n-acetylglucosaminidase encoding gene from the filamentous fungus aspergillus oryzae, and designated it naga. the naga gene encoded a polypeptide of 600 amino acids with significant similarity to glucosaminidases and hexosaminidases of various eukaryotes. a. oryzae strain carrying the naga gene under the control of the improved glaa promoter produced large amounts of beta-n-acetylglucosaminidase in a wheat bran solid culture. the beta-n-acetylglucosaminidase was purified from ... | 2003 | 12723619 |
| substrate specificities of deuterolysin from aspergillus oryzae and electron paramagnetic resonance measurement of cobalt-substituted deuterolysin. | the substrate specificities of deuterolysin, a 19-kda zinc-protease (ec 3.4.24.39) from aspergillus oryzae, were investigated at ph 9.0 with various fluorogenic acyl-peptide-4-methylcoumaryl-7-amides (peptide-mcas). n-butoxycarbonyl-arg-val-arg-arg-mca was the best substrate for deuterolysin. we therefore measured its kinetic parameters. deuterolysin had high activity toward the peptide bonds next to pairs of basic residues in calf thymus histone h4. the specificity of cobalt-substituted deutero ... | 2003 | 12728984 |
| in vivo visualization of the distribution of a secretory protein in aspergillus oryzae hyphae using the rnta-egfp fusion protein. | a fusion gene encoding ribonuclease t1-egfp (rnta-egfp) was constructed and expressed to use it as a tool for studies on the secretory pathway in aspergillus oryzae. the successful secretion of the intact rnta-egfp fusion protein was detected by fluorescence measurement and western analysis. with use of the rnta-egfp system, we were able to see high fluorescence at hyphal tips and observe concentrated fluorescence at septa in basal cells during growth at optimal conditions. cold or heat shock du ... | 2003 | 12729022 |
| calotropis procera (sodom apple)--a potential material for enzyme purification. | a simple method based on precipitation with calotropis procera latex was developed for the purification of crude enzyme from fermentation broth. c. procera latex (10(-2) dilution) clarified and concentrated the crude amylase of aspergillus oryzae 4-fold with 97% recovery of the initial amylase activity in the filtrate in a single step operation. the latex was stable at ph < or = 4.5 and there was no significant difference (p < or = 0.05) in the purification potential of the latex at 4 and 28 deg ... | 2003 | 12733587 |
| molecular characterisation and expression analysis of the first hemicellulase gene (bxl1) encoding beta-xylosidase from the thermophilic fungus talaromyces emersonii. | the gene coding for beta-xylosidase, bxl1, has been cloned from the thermophilic filamentous fungus, talaromyces emersonii. this is the first report of a hemicellulase gene from this novel source. at the genomic level, bxl1 consists of an open reading frame of 2388 nucleotides with no introns that encodes a putative protein of 796 amino acids. the bxl1 translation product contains a signal peptide of 21 amino acids that yields a mature protein of 775 amino acids, with a predicted molecular mass ... | 2003 | 12763033 |
| gene cloning and functional analysis of a second delta 6-fatty acid desaturase from an arachidonic acid-producing mortierella fungus. | we demonstrated that mortierella alpina 1s-4 has two delta 6-desaturases, which are involved in the desaturation of linoleic acid to gamma-linolenic acid. for one of the two delta 6-desaturases, designated as delta 6i, gene cloning and its heterologous expression in a fungus, aspergillus oryzae, has previously been reported. in addition, we indicated in this paper that there is an isozyme of the two delta 6-desaturases, designated as delta 6ii, in m. alpina 1s-4. the predicted amino acid sequenc ... | 2003 | 12784608 |
| a novel heterofunctional epoxy-amino sepabeads for a new enzyme immobilization protocol: immobilization-stabilization of beta-galactosidase from aspergillus oryzae. | the properties of a new and commercially available amino-epoxy support (amino-epoxy-sepabeads) have been compared to conventional epoxy supports to immobilize enzymes, using the beta-galactosidase from aspergillus oryzae as a model enzyme. the new support has a layer of epoxy groups over a layer of ethylenediamine that is covalently bound to the support. this support has both a great anionic exchanger strength and a high density of epoxy groups. epoxy supports require the physical adsorption of ... | 2003 | 12790680 |
| pulsed feeding during fed-batch aspergillus oryzae fermentation leads to improved oxygen mass transfer. | productivity in many fungal fermentations is detrimentally affected by high broth viscosity and consequent reduced oxygen mass transfer capacity. the goal here was to determine whether pulsed feeding of limiting carbon in a fungal fermentation could lead to reduced viscosity and improved oxygen mass transfer. as a model, an industrially relevant recombinant strain of aspergillus oryzae was grown in carbon-limited, fed-batch mode. maltodextrin was used as a carbon source and was added either cont ... | 2003 | 12790687 |
| preventive effect of fermented brown rice and rice bran on diethylnitrosoamine and phenobarbital-induced hepatocarcinogenesis in male f344 rats. | epidemiological and preclinical studies have suggested that nutrition plays an important role in the etiology of cancer. our group previously demonstrated that rice germ or fermented brown rice has a preventive effect on colorectal carcinogenesis. the experiment described here was examined for the potential anticancer properties of brown rice fermented by aspergillus oryzae (fbra) in male f344 rats using inhibition of diethylnitrosoamine (den) and phenobarbital (pb)-induced hepatocarcinogenesis ... | 2003 | 12792738 |
| substrate aggregation due to aerial hyphae during discontinuously mixed solid-state fermentation with aspergillus oryzae: experiments and modeling. | solid-state fermentation (ssf) is prone to process failure due to channeling caused by evaporative cooling and the formation of an interparticle mycelium network. mixing is needed to break the mycelium network and to avoid such failure. this study presents the first attempt to quantify and predict the effect of mycelium bonds on particle mixing and vice versa. we developed a novel experimental set-up to measure the tensile strength of hyphal bonds in ssf: aspergillus oryzae was cultivated betwee ... | 2003 | 12827692 |
| construction of an efficient expression system for aspergillus isopullulanase in pichia pastoris, and a simple purification method. | aspergillus niger atcc 9642 isopullulanase (ipu) was heterologously expressed by pichia pastoris gs115 under three different signal sequences of saccharomyces cerevisiae acid phosphatase, s. cerevisiae alpha-factor prepro peptide, and a. niger isopullulanase. one-step purification using lectin con a affinity chromatography yielded recombinant ipu (ipu-pp) with high purity. ipu-pp had a higher carbohydrate content than native ipu and ipu-ao expressed in a. oryzae m-2-3. ipu-pp hydrolyzed various ... | 2003 | 12834298 |
| authentic and recombinant bilirubin oxidases are in different resting forms. | myrothecium verrucaria bilirubin oxidase expressed in aspergillus oryzae is in a resting form different from that of the authentic bilirubin oxidase, but reaches the resting form of the authentic enzyme after one cycle of reduction and reoxidation with dioxygen as shown by the absorption and electron paramagnetic resonance spectra. | 2003 | 12834300 |
| cloning, sequencing, and heterologous expression of a cellobiohydrolase cdna from the basidiomycete corticium rolfsii. | from a corticium rolfsii cdna library, a clone homologous to other fungal cellobiohydrolase (cbh1) genes was isolated using the polymerase chain reaction. in the nucleotide sequence, one 1.6 kb long open reading frame coding for a polypeptide of 530 amino acid residues was detected which showed 64% identity with cbh1 of phanerochaete chrysosporium. with expression of the 1.8 kb cdna using the aspergillus oryzae expression system, we detected microcrystalline cellulose (avicel) hydrolyzing activi ... | 2003 | 12843660 |
| type iii cu mutants of myrothecium verrucaria bilirubin oxidase. | type iii cu ligand, his456 and his458, of myrothecium verrucaria (mt-1) bilirubin oxidases (bo) [ec 1.3.3.5] were doubly mutated as to lys, asp, and val. in spite of perturbation of the type iii cu centers, these mutants were pale blue or colourless when isolated. however, they became intense blue on reaction with reducing agents such as dithionite, ascorbate, hexacyanoferrate(ii), and octacyanotangstate(iv) under air, or with an oxidizing agent such as hexacyanoferrate(iii), indicating that the ... | 2003 | 12869533 |
| active elimination of causative pcdfs/dds congeners of yusho by one year intake of fbra in japanese people. | thirty-five years have been passing since the outbreak of kanemi rice oil poisoning, namely, yusho in the western japan. however, even now the patients with yusho have been still suffering from several objective and subjective symptoms. in order to improve or, if possible, to cure the such symptoms, the most important therapeutic treatment is considered to actively excrete the most toxic causative pcdfs/dds congeners, that is, 2,3,4,7,8-pentachlorodibenzofuran (pencdf) and 1,2,3,6,7,8-hexachloro ... | 2003 | 12872712 |
| novel role of cytoplasmic dynein motor in maintenance of the nuclear number in conidia through organized conidiation in aspergillus oryzae. | cytoplasmic dynein is a minus-end-directed, microtubule-dependent motor protein complex. dhca, cytoplasmic dynein heavy chain in aspergillus oryzae, contained four p-loops involved in atp binding which were conserved as in cytoplasmic dynein heavy chains of other organisms. the amino acid sequence of a. oryzae dhca was similar to cytoplasmic dynein heavy chains from other organisms except for the n-terminus of saccharomyces cerevisiae dyn1. disruption of dhca gene in the region encoding four p-l ... | 2003 | 12878196 |
| upregulation of promoter activity of the aspergillus oryzae xylanase gene by site-directed mutagenesis. | in aspergillus oryzae, inductive expression of the xynf1 gene is mediated by a transcriptional activator, aoxlnr. promoter activity of the xynf1 gene, monitored by beta-galactosidase activity, was successfully upregulated by mutating two non-canonical aoxlnr binding sequences to what is thought to be the strongest sequence. transformants carrying three canonical binding sequences in the promoter region produced enzyme 2.8 times more active (33,000 units mg(-1) protein) than that of transformants ... | 2003 | 12882555 |
| solid-phase reducing agents as alternative for reducing disulfide bonds in proteins. | disulfide reduction of kluyveromyces lactis and aspergillus oryzae beta-galactosidases and beta-lactoglobulin was assessed. reduction was performed using one of two thiol-containing agents: dithiothreitol (dtt) or thiopropyl-agarose with a high degree of substitution (1000 micromol of sh groups/g of dried gel). both reductants allowed an increase of three- (for k. lactis beta-galactosidase) and fourfold (for a. oryzae beta-galactosidase) in the initial content of sh groups in the lactases. nearl ... | 2003 | 12909729 |
| thermal stability of alpha-amylase from aspergillus oryzae entrapped in polyacrylamide gel. | to determine the suitability as a time-temperature indicator for dielectric pasteurization processes, the thermal stability (50-75 degrees c) of aspergillus oryzae alpha-amylase immobilized in polyacrylamide gel in phosphate buffer, mashed potatoes, and minced shrimp was examined. changing the cross-linking agent concentration from 3.3 to 5.3% and adding 2% salt did not markedly affect the thermal stability of the immobilized alpha-amylase. thermal inactivation was first order, and immobilizatio ... | 2003 | 12926898 |
| isofagomine lactams, synthesis and enzyme inhibition. | the synthesis of isofagomine lactams (2-oxoisofagomines) corresponding to the biologically important hexoses is presented. the d-glucose/d-mannose analogue (3s,4r,5r)-3,4-dihydroxy-5-hydroxymethylpiperidin-2-one (9) was synthesised in 9 steps from d-arabinose, the d-galactose analogue (3s,4s,5r)-3,4-dihydroxy-5-hydroxymethylpiperidin-2-one (10) was synthesised in 11 steps from d-arabinose and the l-fucose analogue (3r,4r,5r)-3,4-dihydroxy-5-methylpiperidin-2-one (11) was synthesised in 12 steps ... | 2003 | 12929423 |
| preferential binding of two compatible solutes to the glycan moieties of peniophora lycii phytase. | regulation of hydration behavior, and the concomitant effects on solubility and other properties, has been suggested as a main function of protein glycosylation. in this work, we have studied the hydration of the heavily glycosylated peniophora lycii phytase in solutions (0.15-1.1 m) of the two compatible solutes glycerol and sorbitol. osmometric measurements showed that glycerol preferentially binds to phytase (i.e., glycerol-glycoprotein interactions are more favorable than water-glycoprotein ... | 2003 | 12939159 |
| pulsed feeding during fed-batch fungal fermentation leads to reduced viscosity without detrimentally affecting protein expression. | the goal in this study was to determine if pulsed addition of substrate could be used to alter filamentous fungal morphology during fermentation, to result in reduced broth viscosity. in all experiments, an industrially relevant strain of aspergillus oryzae was grown in 20-liter fermentors. as a control, cultures were fed limiting substrate (glucose) continuously. tests were performed by altering the feeding strategy so that the same total amount of glucose was fed in repeated 300-s cycles, with ... | 2003 | 12474257 |
| effect of deletion of chitin synthase genes on mycelial morphology and culture viscosity in aspergillus oryzae. | the objective of this study was to quantify the effect of disrupting two chitin synthases, chsb and csma, on the morphology and rheology during batch cultivation of aspergillus oryzae. the rheological properties were characterized in batch cultivations at different biomass concentrations (from 3.4-22.5 g kg(-1) biomass) and the power-law model adequately described the rheological properties. in the cultivations there were pellets, clumps, and freely dispersed hyphal elements. the different morph ... | 2003 | 12514801 |
| aorsin, a novel serine proteinase with trypsin-like specificity at acidic ph. | a proteinase that hydrolyses clupeine and salmine at acidic ph, called aorsin, was found in the fungus aspergillus oryzae. purified aorsin also hydrolysed benzyloxycarbonyl-arg-arg-4-methylcoumaryl-7-amide optimally at ph 4.0. the specificity of aorsin appeared to require a basic residue at the p(1) position and to prefer paired basic residues. aorsin activated plasminogen and converted trypsinogen to trypsin. the trypsin-like activity was inhibited strongly by antipain or leupeptin, but was not ... | 2003 | 12519073 |
| dipeptidyl peptidase iv on activated t cells as a target molecule for therapy of rheumatoid arthritis. | the extracellular domain of the t cell co-stimulatory molecule cd26 possesses dipeptidyl peptidase iv (dp iv) enzyme activity. activated t cells are known to increase expression of cell surface dp iv and some specific inhibitors of this enzyme have been reported to suppress t cell function. previously we have identified a dp iv inhibitor, designated tmc-2, found in culture supernatant of aspergillus oryzae. administration of tmc-2 to rats with adjuvant arthritis caused marked suppression of paw ... | 2003 | 12519388 |
| change in maltose- and soluble starch-hydrolyzing activities of chimeric alpha-glucosidases of mucor javanicus and aspergillus oryzae. | the chimeric alpha-glucosidases of mucor javanicus and aspergillus oryzae, which has high activity toward not only maltooligosaccharides but also soluble starch and has high activity toward maltooligosaccharides but faint activity toward soluble starch, respectively, were constructed by shuffling the c-terminal regions where low homology is observed between the two enzymes. the chimera genes were expressed in pichia pastoris to produce and secrete the enzymes that have predicted molecular masses ... | 2003 | 12535604 |
| sequence comparison of aflr from different aspergillus species provides evidence for variability in regulation of aflatoxin production. | aflatoxin contamination of foods and feeds is a world-wide agricultural problem. aflatoxin production requires expression of the biosynthetic pathway regulatory gene, aflr, which encodes a cys6zn2-type dna-binding protein. homologs of aflr from aspergillus nomius, bombycis, parasiticus, flavus, and pseudotamarii were compared to investigate the molecular basis for variation among aflatoxin-producing taxa in the regulation of aflatoxin production. variability was found in putative promoter consen ... | 2003 | 12553937 |
| molecular breeding of the mureka-non-forming sake koji mold from aspergillus oryzae by the disruption of the mrea gene. | mureka-non-forming sake koji molds were constructed from an aspergillus oryzae industrial strain by the disruption of the mrea gene using a host-vector system with the ptra gene as a dominant selectable marker. all of the mrea gene disruptants obtained retained the advantages of the host strain in terms of the brewing characteristics, while their isoamyl alcohol oxidase (iaaod) activities were significantly lower than that of the host strain. sake brewing was successfully carried out using the k ... | 2003 | 16233364 |
| dffa gene from aspergillus oryzae encodes l-ornithine n5-oxygenase and is indispensable for deferriferrichrysin biosynthesis. | we identified and analyzed the dffa gene from aspergillus oryzae which encodes l-ornithine n5-oxygenase involved in the biosynthesis of deferriferrichrysin, a type of siderophore which is a low-molecular-weight iron chelating compound. from among more than 20,000 clones in an a. oryzae est (expressed sequence tag) library, we found only one clone encoding a protein that exhibited homology to theustilago maydis sid1 protein (sid1) and pseudomonas aeruginosa pvda protein (pvda), both known as the ... | 2003 | 16233371 |
| synergistic degradation of arabinoxylan with alpha-l-arabinofuranosidase, xylanase and beta-xylosidase from soy sauce koji mold, aspergillus oryzae, in high salt condition. | this study addresses induction and some properties of alpha-l-arabinofuranosidase from a soy sauce koji mold, aspergillus oryzae hl15, cultured on solid and liquid media. alpha-l-arabinofuranosidase was induced by soybean polysaccharide and secreted into media on solid cultivation; the enzyme was associated with mycelium as a cell-wall-bound form in liquid cultivation. a major alpha-l-arabinofuranosidase, which was purified homogeneously on sds-page, showed highest activity in the presence of 10 ... | 2003 | 16233386 |
| production and properties of phytase and acid phosphatase from a sake koji mold, aspergillus oryzae. | we identified three types of acid phosphatase (acp-i, acp-ii, and acp-iii) produced by aspergillus oryzae in a submerged culture using only phytic acid as the phosphorous substrate. the optimum ph for the activities of the three enzymes was in the range of 4.5 to 5.5. analysis of the substrate specificities of these enzymes revealed that acp-i and acp-iii were acid phosphatases, and acp-ii was a phytase. these enzymes were produced during different periods of mycelial growth: acp-ii was produced ... | 2003 | 16233418 |
| production of two types of phytase from aspergillus oryzae during industrial koji making. | in our previous study, it was determined that phytase produced by aspergillus oryzae plays an important role in supplying phosphate to yeast in the process of making sake. during koji making, two types of phytase (phy-i and phy-ii) are produced. the purified phytases have high thermal and ph stability, in comparison to phytase purified from a submerged culture (acp-ii). in the present study, phy-i and phy-ii retained their activities for 45 h. the nh2-terminal sequence of phy-1, which is eight a ... | 2003 | 16233440 |
| overexpression of aspergillus aculeatus cellobiohydrolase i in aspergillus oryzae. | to express the cbhi gene, encoding aspergillus aculeatus cellobiohydrolase i (cbhi), in aspergillus oryzae, a plasmid was constructed. the strain that displayed the strongest cbhi activity among the transformants produced about 941 mg/l in liquid culture. it was confirmed by a pcr method that the plasmid was integrated at the niad locus. | 2003 | 16233469 |
| cloning, heterologous expression, and characterization of thielavia terrestris glucoamylase. | thielavia terrestris is a soil-borne thermophilic fungus whose molecular/ cellular biology is poorly understood. only a few genes have been cloned from the thielavia genus. we detected an extracellular glucoamylase in culture filtrates of t. terrestris and cloned the corresponding glaa gene. the coding region contains five introns. based on the amino acid sequence, the glucoamylase was 65% identical to neurospora crassa glucoamylase. sequence comparisons suggested that the enzyme belongs to the ... | 2003 | 14665735 |
| thiamine-regulated gene expression of aspergillus oryzae thia requires splicing of the intron containing a riboswitch-like domain in the 5'-utr. | exogenous thiamine regulates aspergillus oryzae thia, which is involved in thiamine synthesis. one of the two introns in its 5'-untranslated region (5'-utr) contains motifs (regions a and b) highly conserved among fungal thiamine biosynthesis genes. deletion of either region relieved the repression by thiamine and thiamine inhibited intron splicing, suggesting that regions a and b are required for efficient splicing. furthermore, transcript splicing was essential for thia gene expression. these ... | 2003 | 14675766 |
| [analysis of kojic acid in aspergillus oryzae ferment by ion-pair reversed-phase high performance liquid chromatography]. | a new and an efficient method for analyzing kojic acid in aspergillus oryzae ferment by ion-pair reversed-phase high performance liquid chromatography was developed. the kojic acid was well separated on a c18 column using methanol-0.01 mol/l disodium hydrogen phosphate and 2 mmol tetrabutylammonium bromide (4:96) as the mobile phase, followed by an uv detection at 226 nm. the detection limit was 0.012 microgram/ml. the spiked recoveries in wort-yeast culture, wort-peptone culture and potato-yeas ... | 2003 | 14535109 |
| study of the inhibition of four alpha amylases by acarbose and its 4iv-alpha-maltohexaosyl and 4iv-alpha-maltododecaosyl analogues. | acarbose analogues, 4iv-maltohexaosyl acarbose (g6-aca) and 4iv-maltododecaosyl acarbose (g12-aca), were prepared by the reaction of cyclomaltodextrin glucanyltransferase with cyclomaltohexaose and acarbose. the inhibition kinetics of acarbose and the two acarbose analogues were studied for four different alpha-amylases: aspergillus oryzae, bacillus amyloliquefaciens, human salivary, and porcine pancreatic alpha-amylases. the three inhibitors showed mixed, noncompetitive inhibition, for all four ... | 2003 | 14499573 |
| protein purification, cdna cloning and characterization of a protease inhibitor from the indian tasar silkworm, antheraea mylitta. | an inhibitor of aspergillus oryzae fungal protease was purified to homogeneity from the hemolymph of fifth instar larvae of antheraea mylitta by ammonium sulfate precipitation, anion exchange and gel filtration (fplc) chromatography, and termed as amfpi-1. the extent of purification was checked by two-dimensional gel electrophoresis, and the molecular weight of purified inhibitor was determined by sds-page as 10.4 kda. fifteen n-terminal amino acid sequences of this protein were determined, and ... | 2003 | 14505696 |
| fungal beta-n-acetylhexosaminidases with high beta-n-acetylgalactosaminidase activity and their use for synthesis of beta-galnac-containing oligosaccharides. | about 60 fungal strains were tested for production of extracellular beta-n-acetylhexosaminidases. a unique beta-n-acetylhexosaminidase with the beta-galnac-ase/beta-glcnac-ase ratio of 2.3-2.8 was found in the culture filtrates of some strains of penicillium oxalicum. addition of 20% (w/v) mgso(4) increased the beta-galnac-ase/beta-glcnac-ase ratio to the value of 3.35. cultivation conditions influence this ratio as well. beta-n-acetylhexosaminidases from p. oxalicum ccf 2430 and aspergillus ory ... | 2003 | 12681926 |
| functional analysis of the calcineurin-encoding gene cnaa from aspergillus oryzae: evidence for its putative role in stress adaptation. | the presence of putative stre (stress response regulatory element) and hsf (heat-shock factor) transcription factor binding sites in the promoter region of the gene encoding calcineurin ( cnaa) from aspergillus oryzae implicated a probable role for calcineurin in the stress response. the activity of calcineurin was enhanced during growth of the wild-type strain in the presence of 1 m nacl (2.6-fold), at alkaline ph 10.0 (2.9-fold) and at 37 degrees c (1.6-fold). the induction of cnaa antisense e ... | 2003 | 12709783 |
| characteristics of a newly isolated fungus geotrichum candidum dec 1 with broad degradation spectrum of xenobiotic compounds. | a newly isolated fungus, geotrichum candidum dec 1 (abbreviated as dec 1), was found to have the ability to degrade many xenobiotic compounds such as synthetic dyes, food coloring agents, molasses, organic halogens, lignin and kraft pulp effluents. the broad spectrum of the degradation of these compounds are associated mainly with peroxidases produced by the fungus. | 2003 | 15296177 |
| influence of rumen protein degradability on productive and reproductive performance in buffalo cows. | the present study aimed to ascertain the influence of crude protein (cp) digestibility in the rumen on the quantity and quality of milk production and reproductive performance, blood (bu) and milk (mu) urea, haematological profile and vaginal mucus urea, ammonia and potassium of buffalo cows. lactating buffaloes (n = 84), 60 days in milk, were randomly subdivided into group c (control, n = 42) and group t (fed a diet supplemented with aspergillus oryzae, n = 42). in three fistulated buffalo, the ... | 2003 | 15141439 |
| transformation of aspergillus aculeatus using the drug resistance gene of aspergillus oryzae and the pyrg gene of aspergillus nidulans. | transformation systems for aspergillus aculeatus has been developed, based on the use of the pyrithiamine resistance gene of aspergillus oryzae and the orotidine-5'-monophosphate decarboxylase gene (pyrg) of aspergillus nidulans. an a. aculeatus mutant which can be transformed effectively by the a. nidulans pyrg gene was isolated as a transformation host. this is the first report of transformation of a. aculeatus. | 2003 | 14730150 |
| [the action of s1 nuclease and a cloning strategy for microcircular dnas]. | s1 nuclease (from aspergillus oryzae) is a specific enzyme to degrade single stranded dna or rna molecules. it has been reported to be able to convert superhelical circular dna molecules into open circle or linear forms under certain conditions, but this function has not been well explored. in order to use the action of s1 nuclease to linearize circular dna and develop a novel way of cloning microcircular dnas, the puc19 was used to investigate the relationship between the linearization efficien ... | 2003 | 15966330 |
| production of cellulose- and xylan-degrading enzymes by a koji mold, aspergillus oryzae, and their contribution to the maceration of rice endosperm cell wall. | the production of cellulose- (cel), xylan- (xyl), and pectin-degrading enzymes (pec) by a koji mold, aspergillus oryzae, was studied, and their contributions to the maceration of the rice endosperm cell wall were investigated with regard to the utilization of available rice in the sake mash. the sake koji mold showed higher cel and xyl productivities, whereas the miso and soy sauce koji molds showed higher pec productivity. statistical analyses indicated that cel and xyl contribute predominantly ... | 2002 | 16233157 |
| simultaneous display of bacterial and fungal lipases on the cell surface of bacillus subtilis. | a small cell wall-binding domain (cwb(c)) of the bacillus subtilis peptidoglycan hydrolase cwlc fused to b. subtilis lipase b was able to be localized on the cell wall of b. subtilis. with the aim of developing an efficient lipid-hydrolyzing bacterium with two different lipases on the cell surface, plasmids possessing genes for lipb-cwb(c) and cwb(b)-cutl (the cell wall-binding domain of cwlb fused to the aspergillus oryzae lipolytic enzyme cutl) were constructed. b. subtilis harboring these pla ... | 2002 | 16233158 |
| properties of cellulose-degrading enzymes from aspergillus oryzae and their contribution to material utilization and alcohol yield in sake mash fermentation. | four cellulose-degrading enzymes were identified in a solid-state culture of aspergillus oryzae. the three major enzymes were purified and named cel-1, cel-2, and cel-3, respectively. the molecular weights were determined to be 62, 120, and 34 kda, respectively. the optimum temperature of cel-3 activity was higher than that of the other enzymes. an acidic ph was found to be more suitable for cel-1 activity than for the other enzymes, and cel-3 was more stable under acidic conditions than the oth ... | 2002 | 16233235 |
| specific expression and temperature-dependent expression of the acid protease-encoding gene (pepa) in aspergillus oryzae in solid-state culture (rice-koji). | the synthesis of acid protease in rice-koji is important for sake brewing. northern blot analysis was carried out to study the transcriptional regulation of acid protease-encoding gene (pepa in aspergillus orytae. the pepa gene was not expressed in submerged culture, while it was expressed when cultured on steamed rice. additionally, the culture at high temperature (>38 degrees c) caused a marked decrease in transcription level of pepa, although the alpha-amylase (amyb) and actin genes were expr ... | 2002 | 16233250 |
| enzymatic synthesis of l-menthyl alpha-maltoside and l-menthyl alpha-maltooligosides from l-menthyl alpha-glucoside by cyclodextrin glucanotransferase. | l-menthyl alpha-d-glucopyranosyl-(1-->4)-alpha-d-glucopyranoside (alpha-meng2), a novel glycoside of l-menthol, was synthesized enzymatically and its physicochemical properties were characterized. production of alpha-meng2 from l-menthyl alpha-d-glucopyranoside (alpha-meng) was attempted since we had already succeeded in the high-yield production of alpha-meng using a xanthomonas campestris enzyme (nakagawa h., et al. j. biosci. bioeng., 89, 138-144, 2000). through production tests on enzymes, i ... | 2002 | 16233280 |
| recent studies of protein secretion by filamentous fungi. | filamentous fungi have been widely exploited for the homologous and heterologous protein production, because of the high capacity of their protein secretion machinery. however, the production of heterologous proteins is often limited while the production of homologous proteins can be very high. various researches have reported the methods for overcoming this problem and some techniques, such as the fusion gene system, improve the production of heterologous proteins. recently, the molecular biolo ... | 2002 | 16233346 |
| [improvement of recombinant xylanase xynf1 from aspergillus oryzae expressed in pichia pastoris by site-directed mutagenesis]. | the recombinant xylanase xynf1 from aspergillus oryzae expressed in the methylotrophic yeast pichia pastoris was improved by site-directed mutagenesis. the mutant i156a secreted about 47.7 u/ml xylanase xynf1 in the bmmy medium. the mutant xylanase xynf1 was purified by ion exchange and gel filtration chromatographies. the molecular weight of purified xynf1 was 35 kd. xynf1 was stable between ph 4-9 and its optimum ph was 7.0. the optimum temperature of xynf1 was 45 degrees c and it was stable b ... | 2002 | 12557547 |
| [cloning and sequencing of lactase gene from aspergillus candidus]. | genomic dna and cdna sequences of lactase from aspergillus candidus were cloned. sequences analysis revealed that the genomic dna was 3458 bp containing eight introns, cdna was 3015 bp and encoding a polypeptide of 1005 amino acid residues. signal peptide was 19 amino acid residues, eleven potential n-glycosylation sites were assumed. comparing the gene cdna and amino acid sequences with other lactase sequences from various sources, it showed a very low homology with most of other sequences. alt ... | 2002 | 12561200 |
| altering the expression of two chitin synthase genes differentially affects the growth and morphology of aspergillus oryzae. | in aspergillus oryzae, one full-length chitin synthase (chsb) and fragments of two other chitin synthases (csma and chsc) were identified. the deduced amino acid sequence of chsb was similar (87% identity) to chsb from aspergillus nidulans, which encodes a class iii chitin synthase. the sequence obtained for csma indicated that it had high similarity to class v chitin synthases. chsb and csma disruption strains and a strain in which chsb transcription was controlled were constructed using the ni ... | 2002 | 12480906 |
| estimation of hyphal tensile strength in production-scale aspergillus oryzae fungal fermentations. | fragmentation of filamentous fungal hyphae depends on two phenomena: hydrodynamic stresses, which lead to hyphal breakage, and hyphal tensile strength, which resists breakage. the goal of this study was to use turbulent hydrodynamic theory to develop a correlation that allows experimental data of morphology and hydrodynamics to be used to estimate relative (pseudo) tensile strength (sigma(pseudo)) of filamentous fungi. fed-batch fermentations were conducted with a recombinant strain of aspergill ... | 2002 | 11807755 |
| behaviors of d- and l-lactic acids during the brewing process of sake (japanese rice wine). | the amounts of d- and l-lactic acids during the brewing process of sake were determined by capillary electrophoresis using 2-hydroxypropyl-beta-cyclodextrin as a chiral selector. because l-lactic acid, which prevents the growth of nonuseful microorganisms, is a raw material of sake, the ratio of l-lactic acid to total lactic acid is almost 1.0 at the initial stage of sake brewing. during brewing, the ratio decreased gradually and finally reached 0.39. yeast (saccharomyces cerevisiae) for sake br ... | 2002 | 11829643 |
| intake, digestion, and digestive characteristics of neotyphodium coenophialum-infected and uninfected fescue by heifers offered hay diets supplemented with aspergillus oryzae fermentation extract or laidlomycin propionate. | tarentaise heifers fitted with a rumen cannula (539 +/- 7.5 and 487 +/- 15.7 kg avg initial bw in exp. 1 and 2, respectively) were used in two latin square metabolism experiments having 2 x 2 factorial treatment arrangements to determine the effects of supplementation with aspergillus oryzae fermentation extract (ao) or laidlomycin propionate (lp) on intake, digestion, and digestive characteristics of neotyphodium coenophialum-infected (if) or uninfected (ff) tall fescue (festuca arundinacea) ha ... | 2002 | 11831521 |
| dependence of morphology on agitation intensity in fed-batch cultures of aspergillus oryzae and its implications for recombinant protein production. | we previously reported that, although agitation conditions strongly affected mycelial morphology, such changes did not lead to different levels of recombinant protein production in chemostat cultures of aspergillus oryzae (amanullah et al., 1999). to extend this finding to another set of operating conditions, fed-batch fermentations of a. oryzae were conducted at biomass concentrations up to 34 g dry cell weight/l and three agitation speeds (525, 675, and 825 rpm) to give specific power inputs b ... | 2002 | 11835142 |
| a transcriptional activator, aoxlnr, controls the expression of genes encoding xylanolytic enzymes in aspergillus oryzae. | by deletion across the promoter region of the xynf1 gene encoding the major aspergillus oryzae xylanase, a 53-bp dna fragment containing the xlnr binding sequence ggctaaa as well as two similar sequences was shown to confer xylan inducibility on the gene. complementary and genomic dnas encoding the aspergillus niger xlnr homologous gene, abbreviated aoxlnr, were cloned from a. oryzae and sequenced. aoxlnr comprised 971 amino acids with a zinc binuclear cluster domain at the n-terminal region and ... | 2002 | 11848678 |
| generic model of morphological changes in growing colonies of fungi. | fungal colonies are able to exhibit different morphologies depending on the environmental conditions. this allows them to cope with and adapt to external changes. when grown in solid or semisolid media the bulk of the colony is compact and several morphological transitions have been reported to occur as the external conditions are varied. here we show how a unified simple mathematical model, which includes the effect of the accumulation of toxic metabolites, can account for the morphological cha ... | 2002 | 11863559 |
| immobilization of an l-aminoacylase-producing strain of aspergillus oryzae into gelatin pellets and its application in the resolution of d,l-methionine. | the conditions for immobilization of an l-aminoacylase-producing strain of aspergillus oryzae in gelatin and the enzymic characteristics of the immobilized pellets were studied. the optimal concentrations of gelatin, glutaraldehyde and ethyldiamine and time of immobilization were determined. scanning electron micrographs reveal the cross-linked structure differences between the native and immobilized pellets. optimum ph and temperature of the native and immobilized pellets were determined. effec ... | 2002 | 11916452 |
| metabolic engineering of the morphology of aspergillus oryzae by altering chitin synthesis. | morphology and alpha-amylase production during submerged cultivation were examined in a wild-type strain (a1560) and in strains of aspergillus oryzae in which chitin synthase b (chsb) and chitin synthesis myosin a (csma) have been disrupted (chsb/g and cm101). in a flowthrough cell, the growth of submerged hyphal elements was studied online, making it possible to examine the growth kinetics of the three strains. the average tip extension rates of the cm101 and chsb/g strains were 25 and 88% lowe ... | 2002 | 11916702 |
| the aspergillus niger faeb gene encodes a second feruloyl esterase involved in pectin and xylan degradation and is specifically induced in the presence of aromatic compounds. | the faeb gene encoding a second feruloyl esterase from aspergillus niger has been cloned and characterized. it consists of an open reading frame of 1644 bp containing one intron. the gene encodes a protein of 521 amino acids that has sequence similarity to that of an aspergillus oryzae tannase. however, the encoded enzyme, feruloyl esterase b (faeb), does not have tannase activity. comparison of the physical characteristics and substrate specificity of faeb with those of a cinnamoyl esterase fro ... | 2002 | 11931668 |
| immobilization of beta-galactosidase on fibrous matrix by polyethyleneimine for production of galacto-oligosaccharides from lactose. | the production of galacto-oligosaccharides (gos) from lactose by aspergillus oryzae beta-galactosidase immobilized on cotton cloth was studied. a novel method of enzyme immobilization involving pei-enzyme aggregate formation and growth of aggregates on individual fibrils of cotton cloth leading to multilayer immobilization of the enzyme was developed. a large amount of enzyme was immobilized (250 mg/g support) with about 90-95% efficiency. a maximum gos production of 25-26% (w/w) was achieved at ... | 2002 | 11934291 |
| toxicological studies on polyporus pinsitus laccase expressed by aspergillus oryzae intended for use in food. | the laccase used in the study was produced by submerged fermentation of aspergillus oryzae, containing a gene originating from polyporus pinsitus. laccase is to be employed as a processing aid in the juice industry to make a clear and stable juice. the enzyme was subject to a series of toxicological tests to document its safety in use. it was not mutagenic in the salmonella typhimurium reverse mutation assay, and it did not cause chromosomal aberrations in cultured human lymphocytes. no evidence ... | 2002 | 11962689 |
| antifungal efficacy of bacteria isolated from marine sedentary organisms. | the antibiotic-producing ability of 57 bacteria isolated from 8 marine sedentary organisms, 6 sponges (spirastrella sp., phyllospongia sp., ircinia sp., aaptos sp., azorica sp., axinella sp.), 1 soft coral (lobophytum sp.) and 1 alga (sargassum sp.), was evaluated against 6 phytopathogenic fungi (helminthosporium oryzae, rhizoctonium solani, pyricularia oryzae, fusarium oxysporum, aspergillus oryzae and a. fumigatus). bacteria of the genus bacillus (20%), pseudomonas (33%) and flavobacterium (40 ... | 2002 | 11980270 |
| molecular cloning, characterization, and expression analysis of the xynf3 gene from aspergillus oryzae. | the gene encoding xylanase f3 (xynf3) was isolated from a genomic library of aspergillus oryzae kbn616, used for making shoyu koji. the structural part of xynf3 was found to be 1468 bp. the nucleotide sequence of cdna amplified by rt-pcr showed that the open reading frame of xynf3 was interrupted by ten short introns and encoded 323 amino acids. direct n-terminal amino acid sequencing showed that the precursor of xynf3 had a signal peptide of 22 amino acids. the predicted amino acid sequence of ... | 2002 | 11999400 |
| transformation of aspergillus sp. and trichoderma reesei using the pyrithiamine resistance gene (ptra) of aspergillus oryzae. | a pyrithiamine (pt) resistance gene (ptra) was cloned from a pt resistant mutant of aspergillus oryzae and was useful as a dominant selectable marker for transformation of all a. oryzae wild type strain as well as a. nidulans. for further study, we examined whether or not ptra could be used as the transformation marker in other species of filamentous fungi. two types of plasmid, which contain ptra as a selectable marker, were constructed, and the transformation experiments were done with them. o ... | 2002 | 11999416 |
| functional expression in aspergillus oryzae of p15, a protein with potent neurite-inducing activity in pc12 cells. | we previously reported that a fungal protein, p15, induces neurite outgrowth and differentiation of rat pheochromocytoma pc12 cells through the activation of the ca2+ signaling pathway. we report here the secretory production of p15 in aspergillus oryzae. analysis of culture supernatant of a. oryzae transformed with the gene encoding the p15 precursor tagged with a hemagglutinin (ha) epitope demonstrated that the transformant secreted a protein with an apparent molecular mass of 17.5 kda, which ... | 2002 | 12005070 |
| a simple method for enrichment of uninucleate conidia of aspergillus oryzae. | aspergillus oryzae produces multinucleate conidia, which makes the obtaining of homokaryons labor-intensive. analysis of conidia by flow cytometry clarified the relationship that conidia of lower nuclear number were smaller in size. based on this, we have developed a simple way to enrich uninucleate conidia with a membrane filter. our results also suggest that the method is useful for elimination of heterokaryons. | 2002 | 12005075 |
| cloning and characterization of vmaa, the gene encoding a 69-kda catalytic subunit of the vacuolar h+-atpase during alkaline ph mediated growth of aspergillus oryzae. | screening of a cdna library constructed under alkaline ph mediated growth of aspergillus oryzae implicated a vacuolar h+-atpase gene (vmaa) as a putative candidate involved in alkaline ph adaptation. a. oryzae vmaa genomic dna extended to 2072 bp including three introns and encoded a protein of 605 amino acids. vmaap was homologous to vma-1p from neurospora crassa (71%), vma1p from saccharomyces cerevisiae (69%) and atp6a2 from human (49%). the vmaa cdna complemented s. cerevisiae v-atpase disru ... | 2002 | 12007818 |
| polymerase chain reaction-mediated characterization of molds belonging to the aspergillus flavus group and detection of aspergillus parasiticus in peanut kernels by a multiplex polymerase chain reaction. | the aspergillus flavus group covers species of a. flavus and aspergillus parasiticus as aflatoxin producers and aspergillus oryzae and aspergillus sojae as koji molds. genetic similarity among these species is high, and aflatoxin production of a culture may be affected by cultivation conditions and substrate composition. therefore, a polymerase chain reaction (pcr)-mediated method of detecting the aflatoxin-synthesizing genes to indicate the degree of risk a genotype has of being a phenotypic pr ... | 2002 | 12030297 |
| impact of four (13)c-proline isotope labels on the infrared spectra of ribonuclease t1. | ribonuclease t1 was biosynthesized, with all four prolines (13)c-labeled in the peptide c[double bond]o bond, using a proline auxotrophic yeast strain of saccharomyces cerevisiae. the (13)c- and (12)c-proline isotopomers of ribonuclease t1 were investigated by infrared spectroscopy in the thermally unfolded and natively folded state at 80 and 20 degrees c, respectively. in the thermally unfolded state, both proteins established almost indistinguishable spectral features in the secondary structur ... | 2002 | 12033852 |
| antifungal activity of substituted 8-quinolinol-5- and 7-sulfonic acids: a mechanism of action is suggested based on intramolecular synergism. | 8-quinolinol-5-sulfonic acid was nearly devoid of antimicrobial activity, due to what was believed to be an unfavorable partition coefficient. since twenty six 8-quinolinol-5- and 7-sulfonic acids were available from our previous work, they were tested against six fungi. the 7-chloro and 7-bromo-5-sulfonic acids and the 5-chloro and 5-bromo-7-sulfonic acids showed fungal inhibition within one order of magnitude of that of 8-quinolinol. it is suggested that a nonchelating mechanism is in part res ... | 2002 | 12650598 |
| cloning, nucleotide sequencing, and expression of the beta-galactosidase-encoding gene (laca) from aspergillus oryzae. | laca coding for beta-galactosidase (beta-gal) was cloned from the genomic dna of aspergillus oryzae rib40. there were 9 exons in laca and the coding region of 3,015 bp encoded a protein of 1,005 aa with a deduced molecular mass of 109,898. a. oryzae laca was highly homologous to fungal beta-gals, with the highest aa identity of 70.7% to a. niger laca, and also showed significant identity to acid beta-gals belonging to family 35 glycosyl hydrolases. approximately 10 copies of laca under control o ... | 2002 | 12469296 |
| cloning and nucleotide sequence of the glutamate decarboxylase-encoding gene gada from aspergillus oryzae. | we cloned a genomic dna encoding the glutamate decarboxylase (gad) from aspergillus oryzae using a 200-bp dna fragment as the probe. this dna fragment was amplified by the reverse transcription polymerase chain reaction with mrna of a. oryzae as the template and degenerate primers designed from the conserved amino acid sequence of escherichia coli gad and arabidopsis thaliana gad. nucleotide sequencing analysis showed that the cloned gene (designated gada) encoded 514 amino acid residues and con ... | 2002 | 12596854 |
| protein expression and secretion in the yeast yarrowia lipolytica. | strains and vectors for protein expression and secretion have been developed in the yeast yarrowia lipolytica. host strains were constructed with non-reverting auxotrophic markers, deletions of protease-encoding genes, and carrying a docking platform. to drive transcription, either the synthetic hp4d or the inducible pox2 promoter were used. protein secretion is either directed by the targeting sequence of the alkaline extracellular protease or the extracellular lipase (lip2p) signal sequence. w ... | 2002 | 12702287 |
| aspergillus oryzae in solid-state and submerged fermentations. progress report on a multi-disciplinary project. | we report the progress of a multi-disciplinary research project on solid-state fermentation (ssf) of the filamentous fungus aspergillus oryzae. the molecular and physiological aspects of the fungus in submerged fermentation (smf) and ssf are compared and we observe a number of differences correlated with the different growth conditions. first, the aerial hyphae which occur only in ssfs are mainly responsible for oxygen uptake. second, ssf is characterised by gradients in temperature, water activ ... | 2002 | 12702312 |
| observation of egfp-visualized nuclei and distribution of vacuoles in aspergillus oryzae arpa null mutant. | the arpa gene encoding arp1 (actin-related protein) was previously cloned and characterized from aspergillus oryzae. phenotypes of the arpa null mutant indicate its requirement for normal nuclear distribution and morphology of conidiophores. in this study, we further characterized the function of the arpa gene in distribution of organelles. for further analysis of nuclear migration in living cells, an expression system consisting of a fusion protein of aspergillus nidulans histone h2b and egfp ( ... | 2002 | 11786257 |
| validation of a model for process development and scale-up of packed-bed solid-state bioreactors. | we have validated our previously described model for scale-up of packed-bed solid-state fermenters (weber et al., 1999) with experiments in an adiabatic 15-dm(3) packed-bed reactor, using the fungi coniothyrium minitans and aspergillus oryzae. effects of temperature on respiration, growth, and sporulation of the biocontrol fungus c. minitans on hemp impregnated with a liquid medium were determined in independent experiments, and the first two effects were translated into a kinetic model, which w ... | 2002 | 11787011 |
| physiological characterisation of recombinant aspergillus nidulans strains with different crea genotypes expressing a. oryzae alpha-amylase. | the physiology of three strains of aspergillus nidulans was examined--a crea deletion strain, a wild type crea genotype and a strain containing extra copies of the crea gene, all producing aspergillus oryzae alpha-amylase. the strains were cultured in batch and continuous cultivations and the biomass formation and alpha-amylase production was characterised. overexpression of the crea gene resulted in a lower maximum specific growth rate and a slightly higher repression of the alpha-amylase produ ... | 2002 | 11689252 |
| production of galacto-oligosaccharides from lactose by aspergillus oryzae beta-galactosidase immobilized on cotton cloth. | the production of galacto-oligosaccharides (gos) from lactose by a. oryzae beta-galactosidase immobilized on cotton cloth was studied. the total amounts and types of gos produced were mainly affected by the initial lactose concentration in the reaction media. in general, more and larger gos can be produced with higher initial lactose concentrations. a maximum gos production of 27% (w/w) of initial lactose was achieved at 50% lactose conversion with 500 g/l of initial lactose concentration. tri-s ... | 2002 | 11745169 |
| reactivity and stability of mycelium-bound carboxylesterase from aspergillus oryzae. | the reactivity and thermostability of a novel mycelium-bound carboxylesterase from lyophilized cells of aspergillus oryzae are explored in organic solvent. ethanol acetylation was selected as reference esterification reaction. high carboxylesterase activity cells were used as biocatalyst in batch esterification tests at 12.5 < s(o) < 125 mmol l(-1), 5.0 < x(o) < 30 g l(-1), 0.49 < log p < 4.5 and 30 < t < 80 degrees c, as well as in residual activity tests after incubation at 40 < t < 90 degrees ... | 2002 | 11753931 |
| a polymerase chain reaction-based method for cloning novel members of a gene family using a combination of degenerate and inhibitory primers. | we have developed a novel method for cloning gene family members by using a polymerase chain reaction technique. the method is based on the amplification of a broad range of homologous genes in combination with the specific inhibition of already cloned genes. to accomplish this, we designed degenerate primers to highly conserved regions among the gene family members, and inhibitory primers to the divergent region at the 3'-margin of each degenerate primer. the 5'-end of the inhibitory primer, th ... | 2002 | 12036596 |
| effects of increased impeller power in a production-scale aspergillus oryzae fermentation. | the goal in this study was to determine how increased impeller power affects enzyme expression in large-scale (80 m(3)), fed-batch aspergillus oryzae fermentations. an approximate 50% increase in average impeller power was achieved by increasing impeller diameter approximately 10%, while operating at slightly reduced speed. measured decreases in terminal (95%) mixing time show increased power improved bulk mixing. however, batches operated at increased power had lower recombinant enzyme producti ... | 2002 | 12052056 |
| synthesis and utility of sulfated chromogenic carbohydrate model substrates for measuring activities of mucin-desulfating enzymes. | a chromogenic substrate, 4-nitrophenyl 2-acetamido-2-deoxy-beta-d-glucopyranoside 6-sodium sulfate was synthesized and used in combination with beta-n-acetylhexosaminidase for detection of the sulfatase, mdsa, by release of 4-nitrophenol. mdsa was originally isolated from the bacterium prevotella strain rs2 and is believed to be involved in desulfation of sulfomucins, major components of the mucus barrier protecting the human colon surface. the exo nature of the mdsa sulfatase was indicated by i ... | 2002 | 12062525 |
| preventive effect of fermented brown rice and rice bran against colon carcinogenesis in male f344 rats. | epidemiological and preclinical studies demonstrate that nutrition plays an important role in the etiology of cancer. it has been reported that rice components, especially rice germ plays a key role in prevention of cancer. the experiments described here examined the potential anticancer properties of brown rice fermented by aspergillus oryzae (fbra) in male f344 rats using inhibition of the formation of azoxymethene (aom) induced aberrant crypt foci (acf) and tumors in the colon as the measure ... | 2002 | 12066215 |
| microbiological transformation of l-tyrosine to 3,4-dihydroxyphenyl l-alanine (l-dopa) by a mutant strain of aspergillus oryzae uv-7. | the present study deals with the microbiological transformation of l-tyrosine to 3,4-dihydroxyphenyl l-alanine by a mutant strain of aspergillus oryzae uv-7. sixteen different mutant strains of aspergillus oryzae (gcb-6) were isolated through uv-irradiation. these mutant strains were screened for the production of mold mycelia by submerged fermentation in 250-ml erlenmeyer flasks. of all the mutant strains examined, uv-7 gave maximum production of l-dopa (1.28 mg/ml). the reaction was carried ou ... | 2002 | 12070684 |
| effect of molecular confinement on internal enzyme dynamics: frequency domain fluorometry and molecular dynamics simulation studies. | the tryptophanyl emission decay of the mesophilic beta-galactosidase from aspergillus oryzae free in buffer and entrapped in agarose gel is investigated as a function of temperature and compared to that of the hyperthermophilic enzyme from sulfolobus solfataricus. both enzymes are tetrameric proteins with a large number of tryptophanyl residues, so the fluorescence emission can provide information on the conformational dynamics of the overall protein structure rather than that of the local envir ... | 2002 | 12073936 |
| expression of an alpha-galactosidase from saccharomyces cerevisiae in aspergillus awamori and aspergillus oryzae. | a gene encoding alpha-galactosidase activity was isolated by polymerase chain reaction (pcr) from saccharomyces cerevisiae ncyc686 and separately placed under the control of transcriptional elements regulating alpha-amylase expression in aspergillus oryzae and glucoamylase expression in a. awamori. following transformation of both a. oryzae and a. awamori with their respective expression vectors, induction of heterologous alpha-galactosidase from positively selected clones was effected through t ... | 2002 | 12074058 |
| purification and characterization of an extracellular protease from penicillium chrysogenum pg222 active against meat proteins. | an extracellular protease from penicillium chrysogenum (pg222) isolated from dry-cured ham has been purified. the purification procedure involved several steps: ammonium sulfate precipitation, ion-exchange chromatography, filtration, and separation by high-performance liquid chromatography. based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) analysis and gel filtration, the purified fraction showed a molecular mass of about 35 kda. the hydrolytic properties of the purif ... | 2002 | 12089038 |
| molecular cloning and overexpression of flea gene encoding a fucose-specific lectin of aspergillus oryzae. | a protein from the cell lysate of aspergillus oryzae was purified by column chromatography immobilized with a ferrichrysin (fcy), which is one of the siderophores of a. oryzae. it is produced only in an iron-deficient culture and its molecular weight is estimated as 35,000 by sds-page. two internal amino acid sequences of the protein obtained by lysylendopeptidase digestion were analyzed. molecular cloning shows that it encodes 310 putative amino acid residues separated by 4 introns and is desig ... | 2002 | 12092808 |
| contribution of aerial hyphae of aspergillus oryzae to respiration in a model solid-state fermentation system. | oxygen transfer is for two reasons a major concern in scale-up and process control in industrial application of aerobic fungal solid-state fermentation (ssf): 1) heat production is proportional to oxygen uptake and it is well known that heat removal is one of the main problems in scaled-up fermenters, and 2) oxygen supply to the mycelium on the surface of or inside the substrate particles may be hampered by diffusion limitation. this article gives the first experimental evidence that aerial hyph ... | 2002 | 12115123 |
| biosynthesis of l-dopa by aspergillus oryzae. | the present investigation deals with the biosynthesis of l-dopa by parental (gcb-6) and mutant (uv-7) strains of aspergillus oryzae. there was a marked difference between the mycelial morphology and pellet type of parental and uv-irradiated mutant culture. the mutant strain of a. oryzae uv-6 exhibited pellet-like mycelial morphology and improved tyrosinase activity. mould mycelium was used for biochemical conversion of l-tyrosine to l-dopa because tyrosinase is an intracellular enzyme. the mutan ... | 2002 | 12146638 |
| nonfunctionality of aspergillus sojae aflr in a strain of aspergillus parasiticus with a disrupted aflr gene. | aspergillus sojae belongs to the aspergillus section flavi but does not produce aflatoxins. the functionality of the a. sojae aflr gene (aflrs) was examined by transforming it into an deltaaflr strain of a. parasiticus, derived from a nitrate-nonutilizing, versicolorin a (vera)-accumulating strain. the a. parasiticus aflr gene (aflrp) transformants produced vera, but the aflrs transformants did not. even when aflrs was placed under the control of the amylase gene (amyb) promoter of aspergillus o ... | 2002 | 12147467 |
| [study of immobilization of aspergillus oryzae mycelium aided by artificial neural network]. | aspergillus oryzae mycelium pellets with abundant aminoacylase were cultured by liquid fermentation. mycelium pellets were immobilized by crosslinking method with reagent of gelatin and formaldehyde. basing on the orthogonal design table l16 (4(5)), artificial neural network(ann) model with back-propagation(bp) of error structured 4-10-15-1 was used to optimize the immobilization conditions. and the optimal conditions were obtained. then the activity of the immobilized cells prepared under the o ... | 2002 | 12148288 |
| cloning and structural analysis of bglm gene coding for the fungal cell wall-lytic beta-1,3-glucan-hydrolase bglm of bacillus circulans iam1165. | bacillus circulans iam1165 produces isoforms of beta-1,3-glucan-hydrolases. of these enzymes, the 42-kda enzyme bgim degrades aspergillus oryzae cell walls the most actively. a gene coding for a bgim precursor consisting of 411 amino acid residues was cloned. the 27 n-terminal amino acid sequence of the precursor is a signal peptide. the 141 c-terminal amino acid sequence showed a motif of carbohydrate-binding module family 13. this domain bound to pachyman, lichenan, and a. oryzae cell walls. t ... | 2002 | 12162545 |
| chsz, a gene for a novel class of chitin synthase from aspergillus oryzae. | we cloned and characterized a novel aspergillus oryzae chitin synthase gene, chsz, encoding a polypeptide containing a new myosin motor-like domain in its n-terminal half. alignment analysis revealed that chsz was less homologous to known class v enzymes, except for its probable chitin synthase conserved region in the c-terminal half. we also found a chsy gene and found that chsy showed higher similarity to the class v enzymes than did chsz. phylogenetic analysis clearly demonstrated that the a. ... | 2002 | 12172967 |
| subtractive cloning of cdna from aspergillus oryzae differentially regulated between solid-state culture and liquid (submerged) culture. | in solid-state cultures (sc), aspergillus oryzae shows characteristics such as high-level production and secretion of enzymes and hyphal differentiation with asexual development which are absent in liquid (submerged) culture (lc). it was predicted that many of the genes involved in the characteristics of a. oryzae in sc are differentially expressed between sc and lc. we generated two subtracted cdna libraries with bi-directional cdna subtractive hybridizations to isolate and identify such genes. ... | 2002 | 12172969 |
| statistical media optimization and production of its alpha-amylase from aspergillus oryzae in a bioreactor. | the production of an intermediate temperature-stable (its) alpha-amylase from aspergillus oryzae was studied by using a central composite design with three independent variables, viz., starch, yeast extract, and k(2)hpo(4). the model equation provided a suitable model for the response surface for alpha-amylase production, and, from the optimal concentrations of the medium components, a model was predicted, which was then used for enzyme production in a 150-l bioreactor. in the bioreactor studies ... | 2002 | 12177743 |