Publications

three novel lantibiotics, ticins a1, a3, and a4, have extremely stable properties and are promising food biopreservatives.	lantibiotics are antimicrobial peptides with potential applications as the next generation of antimicrobials in the food industry and/or the pharmaceutical industry. nisin has successfully been used as a food preservative for over 40 years, but its major drawback is its limited stability under neutral and alkaline ph conditions. to identify alternatives with better biochemical properties, we screened more than 100 strains of the bacillus cereus group. three novel lantibiotics, ticins a1 (4,062.9 ...	2015	26231642
potential bio-control agent from rhodomyrtus tomentosa against listeria monocytogenes.	listeria monocytogenes is an important foodborne pathogen implicated in many outbreaks of listeriosis. this study aimed at screening for the potential use of rhodomyrtus tomentosa ethanolic leaf extract as a bio-control agent against l. monocytogenes. twenty-two l. monocytogenes isolates were checked with 16 commercial antibiotics and isolates displayed resistance to 10 antibiotics. all the tested isolates were sensitive to the extract with inhibition zones ranging from 14 to 16 mm. minimum inhi ...	2015	26371033
genes associated with desiccation and osmotic stress in listeria monocytogenes as revealed by insertional mutagenesis.	listeria monocytogenes is a foodborne pathogen whose survival in food processing environments may be associated with its tolerance to desiccation. to probe the molecular mechanisms used by this bacterium to adapt to desiccation stress, a transposon library of 11,700 l. monocytogenes mutants was screened, using a microplate assay, for strains displaying increased or decreased desiccation survival (43% relative humidity, 15°c) in tryptic soy broth (tsb). the desiccation phenotypes of selected muta ...	2015	26025900
intra- and inter-species interactions within biofilms of important foodborne bacterial pathogens.	a community-based sessile life style is the normal mode of growth and survival for many bacterial species. under such conditions, cell-to-cell interactions are inevitable and ultimately lead to the establishment of dense, complex and highly structured biofilm populations encapsulated in a self-produced extracellular matrix and capable of coordinated and collective behavior. remarkably, in food processing environments, a variety of different bacteria may attach to surfaces, survive, grow, and for ...	2015	26347727
ribosome hibernation facilitates tolerance of stationary-phase bacteria to aminoglycosides.	upon entry into stationary phase, bacteria dimerize 70s ribosomes into translationally inactive 100s particles by a process called ribosome hibernation. previously, we reported that the hibernation-promoting factor (hpf) of listeria monocytogenes is required for 100s particle formation and facilitates adaptation to a number of stresses. here, we demonstrate that hpf is required for the high tolerance of stationary-phase cultures to aminoglycosides but not to beta-lactam or quinolone antibiotics. ...	2015	26324267
structure and heme-binding properties of hemq (chlorite dismutase-like protein) from listeria monocytogenes.	chlorite dismutase-like proteins are structurally closely related to functional chlorite dismutases which are heme b-dependent oxidoreductases capable of reducing chlorite to chloride with simultaneous production of dioxygen. chlorite dismutase-like proteins are incapable of performing this reaction and their biological role is still under discussion. recently, members of this large protein family were shown to be involved in heme biosynthesis in gram-positive bacteria, and thus the protein was ...	2015	25602700
pore-forming toxins induce macrophage necroptosis during acute bacterial pneumonia.	necroptosis is a highly pro-inflammatory mode of cell death regulated by rip (or ripk)1 and rip3 kinases and mediated by the effector mlkl. we report that diverse bacterial pathogens that produce a pore-forming toxin (pft) induce necroptosis of macrophages and this can be blocked for protection against serratia marcescens hemorrhagic pneumonia. following challenge with s. marcescens, staphylococcus aureus, streptococcus pneumoniae, listeria monocytogenes, uropathogenic escherichia coli (upec), a ...	2015	26659062
inflammasomes coordinate pyroptosis and natural killer cell cytotoxicity to clear infection by a ubiquitous environmental bacterium.	defective neutrophils in patients with chronic granulomatous disease (cgd) cause susceptibility to extracellular and intracellular infections. microbes must first be ejected from intracellular niches to expose them to neutrophil attack, so we hypothesized that inflammasomes detect certain cgd pathogens upstream of neutrophil killing. here, we identified one such ubiquitous environmental bacterium, chromobacterium violaceum, whose extreme virulence was fully counteracted by the nlrc4 inflammasome ...	2015	26572063
aminopeptidase t of m29 family acts as a novel intracellular virulence factor for listeria monocytogenes infection.	the foodborne pathogen listeria monocytogenes employs a number of virulence determinants including metalloproteases to infect hosts. here for the first time, we identified an m29 family aminopeptidase t (encoded by lmo1603) from l. monocytogenes that possesses a typical feature to catalyze the cleavage of amino acids from peptide substrates, with a preference for arginine. the purified recombinant lmo1603 was activated by fe(3+), zn(2+) and mn(2+), but strongly stimulated by co(2+), indicating t ...	2015	26610705
microinjection of francisella tularensis and listeria monocytogenes reveals the importance of bacterial and host factors for successful replication.	certain intracellular bacteria use the host cell cytosol as the replicative niche. although it has been hypothesized that the successful exploitation of this compartment requires a unique metabolic adaptation, supportive evidence is lacking. for francisella tularensis, many genes of the francisella pathogenicity island (fpi) are essential for intracellular growth, and therefore, fpi mutants are useful tools for understanding the prerequisites of intracytosolic replication. we compared the growth ...	2015	26034213
glutathione activates virulence gene expression of an intracellular pathogen.	intracellular pathogens are responsible for much of the world-wide morbidity and mortality due to infectious diseases. to colonize their hosts successfully, pathogens must sense their environment and regulate virulence gene expression appropriately. accordingly, on entry into mammalian cells, the facultative intracellular bacterial pathogen listeria monocytogenes remodels its transcriptional program by activating the master virulence regulator prfa. here we show that bacterial and host-derived g ...	2015	25567281
multiplex detection of nine food-borne pathogens by mpcr and capillary electrophoresis after using a universal pre-enrichment medium.	routine microbiological quality analyses in food samples require, in some cases, an initial incubation in pre-enrichment medium. this is necessary in order to ensure that small amounts of pathogenic strains are going to be detected. in this work, a universal pre-enrichment medium has been developed for the simultaneous growth of bacillus cereus, campylobacter jejuni, clostridium perfringens, cronobacter sakazakii, escherichia coli, enterobacteriaceae family (38 species, 27 genera), listeria mono ...	2015	26579100
comparative evaluation of the antimicrobial activity of different antimicrobial peptides against a range of pathogenic bacteria.	the rapid emergence of resistance to classical antibiotics has increased the interest in novel antimicrobial compounds. antimicrobial peptides (amps) represent an attractive alternative to classical antibiotics and a number of different studies have reported antimicrobial activity data of various amps, but there is only limited comparative data available. the mode of action for many amps is largely unknown even though several models have suggested that the lipopolysaccharides (lps) play a crucia ...	2015	26656394
an insight into the isolation, enumeration, and molecular detection of listeria monocytogenes in food.	listeria monocytogenes, a foodborne pathogen that can cause listeriosis through the consumption of food contaminated with this pathogen. the ability of l. monocytogenes to survive in extreme conditions and cause food contaminations have become a major concern. hence, routine microbiological food testing is necessary to prevent food contamination and outbreaks of foodborne illness. this review provides insight into the methods for cultural detection, enumeration, and molecular identification of l ...	2015	26579116
protozoan cysts act as a survival niche and protective shelter for foodborne pathogenic bacteria.	the production of cysts, an integral part of the life cycle of many free-living protozoa, allows these organisms to survive adverse environmental conditions. given the prevalence of free-living protozoa in food-related environments, it is hypothesized that these organisms play an important yet currently underinvestigated role in the epidemiology of foodborne pathogenic bacteria. intracystic bacterial survival is highly relevant, as this would allow bacteria to survive the stringent cleaning and ...	2015	26070667
an update on the transport and metabolism of iron in listeria monocytogenes: the role of proteins involved in pathogenicity.	listeria monocytogenes is a gram-positive bacterium that causes a rare but severe human disease with high mortality rate. the microorganism is widespread in the natural environment where it shows a saprophytic lifestyle. in the human body it infects many different cell types, where it lives intracellularly, however it may also temporarily live extracellularly. the ability to survive and grow in such diverse niches suggests that this bacterium has a wide range of mechanisms for both the acquisiti ...	2015	25820385
proteolytic activities expressed by gastrointestinal pathogens bacillus cereus, listeria monocytogenes and enterococcus faecium in different growth phases.	bacterial proteases are implicated in protein quality control, biofilm formation or might have a direct function in pathogenesis by processing virulence factors or cleaving host factors. in recent years, knowledge of proteases expressed by gram-negative pathogens remarkably increased. however, investigation of proteases from gram-positive bacteria is rather rare, but required for the analysis of pathogenesis-relevant proteases. in this study, we extracted and detected proteases from the gastroin ...	2015	26682199
detection of foodborne bacterial pathogens from individual filth flies.	there is unanimous consensus that insects are important vectors of foodborne pathogens. however, linking insects as vectors of the pathogen causing a particular foodborne illness outbreak has been challenging. this is because insects are not being aseptically collected as part of an environmental sampling program during foodborne outbreak investigations and because there is not a standardized method to detect foodborne bacteria from individual insects. to take a step towards solving this problem ...	2015	25742168
comparative study of the effects of citral on the growth and injury of listeria innocua and listeria monocytogenes cells.	this study investigates the effect of citral on growth and on the occurrence of sublethal damage in listeria innocua serovar 6a (cect 910) and listeria monocytogenes serovar 4b (cect 4032) cells that were exposed to citral as a natural antimicrobial agent. two initial inoculum concentrations were considered in this investigation: 10(2) and 10(6) cfu/ml. citral exhibited antilisterial activity against l. innocua and l. monocytogenes, and the observed effects were dependent on the concentration of ...	2015	25643164
phenotypic heterogeneity, a phenomenon that may explain why quorum sensing does not always result in truly homogenous cell behavior.	phenotypic heterogeneity describes the occurrence of "nonconformist" cells within an isogenic population. the nonconformists show an expression profile partially different from that of the remainder of the population. phenotypic heterogeneity affects many aspects of the different bacterial lifestyles, and it is assumed that it increases bacterial fitness and the chances for survival of the whole population or smaller subpopulations in unfavorable environments. well-known examples for phenotypic ...	2015	26025903
temperature affects c-di-gmp signalling and biofilm formation in vibrio cholerae.	biofilm formation is crucial to the environmental survival and transmission of vibrio cholerae, the facultative human pathogen responsible for the disease cholera. during its infectious cycle, v. cholerae experiences fluctuations in temperature within the aquatic environment and during the transition between human host and aquatic reservoirs. in this study, we report that biofilm formation is induced at low temperatures through increased levels of the signalling molecule, cyclic diguanylate (c-d ...	2015	25684220
inactivation of the mapk signaling pathway by listeria monocytogenes infection promotes trophoblast giant cell death.	listeria monocytogenes has a well-characterized ability to cross the placental barrier, resulting in spontaneous abortion and fetal infections. however, the mechanisms resulting in infection-associated abortion are not fully understood. in this study, we demonstrate that the dephosphorylation of mapk family proteins caused by l. monocytogenes infection of trophoblast giant (tg) cells, which are placental immune cells, contributes to infectious abortion. dephosphorylation of c-jun, p38, and erk1/ ...	2015	26528279
interferon γ-induced gtpase promotes invasion of listeria monocytogenes into trophoblast giant cells.	listeria monocytogenes is well known for having the ability to cross the placental barrier, leading to fetal infections and abortion. however, the mechanisms leading to infectious abortion are poorly understood. in this study, we demonstrate that interferon γ-induced gtpase (igtp) contributes to the invasion of l. monocytogenes into trophoblast giant (tg) cells, which are placental immune cells. knockdown of igtp in tg cells decreased the relative efficiencies of l. monocytogenes invasion. moreo ...	2015	25645570
rna helicase important for listeria monocytogenes hemolytic activity and virulence factor expression.	rna helicases have been shown to be important for the function of rna molecules at several levels, although their putative involvement in microbial pathogenesis has remained elusive. we have previously shown that listeria monocytogenes dexd-box rna helicases are important for bacterial growth, motility, ribosomal maturation, and rrna processing. we assessed the importance of the rna helicase lmo0866 (here named csha) for expression of virulence traits. we observed a reduction in hemolytic activi ...	2015	26483402
an hd-domain phosphodiesterase mediates cooperative hydrolysis of c-di-amp to affect bacterial growth and virulence.	the nucleotide cyclic di-3',5'- adenosine monophosphate (c-di-amp) was recently identified as an essential and widespread second messenger in bacterial signaling. among c-di-amp-producing bacteria, altered nucleotide levels result in several physiological defects and attenuated virulence. thus, a detailed molecular understanding of c-di-amp metabolism is of both fundamental and practical interest. currently, c-di-amp degradation is recognized solely among dhh-dhha1 domain-containing phosphodiest ...	2015	25583510
intracellular organisms as placental invaders.	in this article we present a novel model for how the human placenta might get infected via the hematogenous route. we present a list of diverse placental pathogens, like listeria monocytogenes or cytomegalovirus, which are familiar to most obstetricians, but others, like salmonella typhi, have only been reported in case studies or small case series. remarkably, all of these organisms on this list are either obligate or facultative intracellular organisms. these pathogens are able to enter and su ...	2015	27695204
ingested salmonella enterica, cronobacter sakazakii, escherichia coli o157:h7, and listeria monocytogenes: transmission dynamics from adult house flies to their eggs and first filial (f1) generation adults.	the mechanical transmission of pathogenic bacteria by synanthropic filth flies is widely recognized. while many studies report the fate and the temporospatial distribution of ingested foodborne bacteria by filth flies, there is little evidence about the transmission dynamics of ingested foodborne bacteria by adult house flies (musca domestica) to their progeny. in this study, we fed parental house fly adults with food contaminated with low, medium, and high concentrations of salmonella enterica, ...	2015	26228457
effect of atmospheric pressure plasma jet on the foodborne pathogens attached to commercial food containers.	bacterial biofilms are associated with numerous infections and problems in the health care and food industries. the aim of this study was to evaluate the bactericidal effect of an atmospheric pressure plasma (app) jet on escherichia coli o157:h7, listeria monocytogenes, and salmonella typhimurium biofilm formation on collagen casing (cc), polypropylene (pp) and polyethylene terephthalate (pet), which are widely used food container materials. the samples were treated separately with the app jet a ...	2015	26604423
dnase-sensitive and -resistant modes of biofilm formation by listeria monocytogenes.	listeria monocytogenes is able to form biofilms on various surfaces and this ability is thought to contribute to persistence in the environment and on contact surfaces in the food industry. extracellular dna (edna) is a component of the biofilm matrix of many bacterial species and was shown to play a role in biofilm establishment of l. monocytogenes. in the present study, the effect of dnasei treatment on biofilm formation of l. monocytogenes egd-e was investigated under static and dynamic condi ...	2015	26733972
culture negative listeria monocytogenes meningitis resulting in hydrocephalus and severe neurological sequelae in a previously healthy immunocompetent man with penicillin allergy.	a previously healthy 74-year-old caucasian man with penicillin allergy was admitted with evolving headache, confusion, fever, and neck stiffness. treatment for bacterial meningitis with dexamethasone and monotherapy ceftriaxone was started. the cerebrospinal fluid showed negative microscopy for bacteria, no bacterial growth, and negative polymerase chain reaction for bacterial dna. the patient developed hydrocephalus on a second ct scan of the brain on the 5th day of admission. an external ventr ...	2015	26697245
listeria monocytogenes meningitis in an immunosuppressed patient with autoimmune hepatitis and igg4 subclass deficiency.	a 51-year-old caucasian woman with listeria monocytogenes meningitis was treated and discharged after an uncomplicated course. her medical history included immunosuppressive treatment with prednisolone and azathioprine for autoimmune hepatitis. a diagnostic work-up after the meningitis episode revealed that she had low levels of the igg4 subclass. to our knowledge, this is the first case report describing a possible association between autoimmune hepatitis and the occurrence of listeria monocyto ...	2015	26558118
assessment of the microbiological quality of fresh produce on sale in sicily, italy: preliminary results.	fresh produce occupies an increasingly important place in the human food supply because of its health-promoting nutritional properties. most fresh produce is eaten raw or after minimal processing and, consequently, pathogen contamination can represent a serious health risk. there has been an increase in foodborne outbreaks and cases associated with fresh produce, but literature data about the prevalence of pathogen contamination are inconsistent. this study was undertaken to assess the hygienic ...	2015	25984508
identification of a potent microbial lipid antigen for diverse nkt cells.	semi-invariant/type i nkt cells are a well-characterized cd1d-restricted t cell subset. the availability of potent ags and tetramers for semi-invariant/type i nkt cells allowed this population to be extensively studied and revealed their central roles in infection, autoimmunity, and tumor immunity. in contrast, diverse/type ii nkt (dnkt) cells are poorly understood because the lipid ags that they recognize are largely unknown. we sought to identify dnkt cell lipid ag(s) by interrogating a panel ...	2015	26254340
treatment with incomplete freund's adjuvant and listeria monocytogenes delays diabetes via an interleukin-17-secretion-independent pathway.	non-obese diabetes (nod) mice are widely used as an animal model in studies of type i diabetes (tid). treatment with complete freund's adjuvant (cfa) in pro-diabetic nod mice is known to inhibit disease progression by activating cd1d-specific natural killer (nk) t cells and inducing interleukin (il)-17 secretion in innate immune cells. the aim of the present study was to examine the effect of incomplete freund's adjuvant (ifa) and l. monocytogenes treatment on the development of tid in nod mice. ...	2015	26136917
cloning, expression analysis, and rna interference study of a horma domain containing autophagy-related gene 13 (atg13) from the coleopteran beetle, tenebrio molitor.	autophagy is a process that is necessary during starvation, as it replenishes metabolic precursors by eliminating damaged organelles. autophagy is mediated by more than 35 autophagy-related (atg) proteins that participate in the nucleation, elongation, and curving of the autophagosome membrane. in a pursuit to address the role of autophagy during development and immune resistance of the mealworm beetle, tenebrio molitor, we screened atg gene sequences from the whole-larva transcriptome database. ...	2015	26136688
cutting edge: bcl6-interacting corepressor contributes to germinal center t follicular helper cell formation and b cell helper function.	cd4(+) germinal center (gc)-t follicular helper (tfh) cells help b cells become long-lived plasma cells and memory cells. the transcriptional repressor bcl6 plays a key role in gc-tfh formation by inhibiting the expression of genes that promote differentiation into other lineages. we determined whether bcor, a component of a polycomb repressive complex that interacts with the bcl6 btb domain, influences gc-tfh differentiation. t cell-targeted bcor deficiency led to a substantial loss of peptide: ...	2015	25964495
genomes of sequence type 121 listeria monocytogenes strains harbor highly conserved plasmids and prophages.	the food-borne pathogen listeria (l.) monocytogenes is often found in food production environments. thus, controlling the occurrence of l. monocytogenes in food production is a great challenge for food safety. among a great diversity of l. monocytogenes strains from food production, particularly strains belonging to sequence type (st)121 are prevalent. the molecular reasons for the abundance of st121 strains are however currently unknown. we therefore determined the genome sequences of three l. ...	2015	25972859
listeria monocytogenes spreads within the brain by actin-based intra-axonal migration.	listeria monocytogenes rhombencephalitis is a severe progressive disease despite a swift intrathecal immune response. based on previous observations, we hypothesized that the disease progresses by intra-axonal spread within the central nervous system. to test this hypothesis, neuroanatomical mapping of lesions, immunofluorescence analysis, and electron microscopy were performed on brains of ruminants with naturally occurring rhombencephalitis. in addition, infection assays were performed in bovi ...	2015	25824833
validation of a previously developed geospatial model that predicts the prevalence of listeria monocytogenes in new york state produce fields.	technological advancements, particularly in the field of geographic information systems (gis), have made it possible to predict the likelihood of foodborne pathogen contamination in produce production environments using geospatial models. yet, few studies have examined the validity and robustness of such models. this study was performed to test and refine the rules associated with a previously developed geospatial model that predicts the prevalence of listeria monocytogenes in produce farms in n ...	2015	26590280
the impact of carvacrol on ammonia and biogenic amine production by common foodborne pathogens.	the impact of carvacrol at different levels (0.1%, 0.5%, and 1%) on ammonia (amn) and biogenic amines (bas) production by 8 common foodborne pathogens (fbps) (staphylococcus aureus, escherichia coli, klebsiella pneumoniae, enterococcus faecalis, pseudomonas aeruginosa, listeria monocytogenes, aeromonas hydrophila, and salmonella paratyphi a) was studied using a rapid high-performance liquid chromatography method. significant differences among bacteria (p < 0.05) in amn and ba production were obs ...	2015	26580308
molecular characterization of a transmembrane c-type lectin receptor gene from ayu (plecoglossus altivelis) and its effect on the recognition of different bacteria by monocytes/macrophages.	c-type lectin receptors (ctlrs) play vital roles in immune responses as pattern-recognition receptors (prrs). in this study, we identified a novel c-type lectin receptor (pactlrc) gene from ayu, plecoglossus altivelis. predicted pactlrc is a single transmembrane receptor with a typical carbohydrate recognition domain (crd) at its c-terminus. sequence comparison and phylogenetic tree analysis showed that pactlrc was most closely related to atlantic salmon (salmo salar) clrc, but was significantly ...	2015	26010409
efficacy of ultraviolet (uv-c) light in a thin-film turbulent flow for the reduction of milkborne pathogens.	nonthermal technologies are being investigated as viable alternatives to, or supplemental utilization, with thermal pasteurization in the food-processing industry. in this study, the effect of ultraviolet (uv)-c light on the inactivation of seven milkborne pathogens (listeria monocytogenes, serratia marcescens, salmonella senftenberg, yersinia enterocolitica, aeromonas hydrophila, escherichia coli, and staphylococcus aureus) was evaluated. the pathogens were suspended in ultra-high-temperature w ...	2015	25884367
microbial biofilms in seafood: a food-hygiene challenge.	seafood forms a part of a healthy diet. however, seafood can be contaminated with foodborne pathogens, resulting in disease outbreaks. because people consume large amounts of seafood, such disease outbreaks are increasing worldwide. seafood contamination is largely due to the naturally occurring phenomenon of biofilm formation. the common seafood bacterial pathogens that form biofilms are vibrio spp., aeromonas hydrophila, salmonella spp., and listeria monocytogenes. as these organisms pose a gl ...	2015	25846914
aerobic bacterial microbiota isolated from the cloaca of the european pond turtle (emys orbicularis) in poland.	we conducted a comparative analysis of the aerobic cloacal bacteria of european pond turtles (emys orbicularis) living in their natural environment and juvenile turtles reared under controlled conditions in a breeding center. we included 130 turtles in the study. the aerobic bacteria isolated from the cloaca of the juvenile turtles were less diverse and more prevalent than the bacteria isolated from free-living adults. we isolated 17 bacterial species from juvenile captive turtles, among which t ...	2015	25380369
hygienisation and nutrient conservation of sewage sludge or cattle manure by lactic acid fermentation.	manure from animal farms and sewage sludge contain pathogens and opportunistic organisms in various concentrations depending on the health of the herds and human sources. other than for the presence of pathogens, these waste substances are excellent nutrient sources and constitute a preferred organic fertilizer. however, because of the pathogens, the risks of infection of animals or humans increase with the indiscriminate use of manure, especially liquid manure or sludge, for agriculture. this p ...	2015	25786255
biological activity of the essential oils from cinnamodendron dinisii and siparuna guianensis.	this study had analyzed the antibacterial, antifungal and trypanocidal activity of the essential oils from cinnamodendron dinisii schwacke (canellaceae) and siparuna guianensis aublet (siparunaceae). the essential oils were obtained from fresh leaves by hydrodistillation, using a modified clevenger apparatus. chemical analysis by gas-liquid chromatography coupled to mass spectrometry (gc-ms) showed that these essential oils are rich in monoterpene and sesquiterpene hydrocarbons. activity against ...	2015	26221107
antimicrobial activity of neo-clerodane diterpenoids isolated from lamiaceae species against pathogenic and food spoilage microorganisms.	antimicrobial activity of nineteen neo-clerodane diterpenoids, isolated from the acetone extracts of the aerial parts of scutellaria and salvia species (lamiaceae) were tested against thirteen strains belonging to nine different species of pathogenic and food spoilage bacteria aeromonas hydrophila, bacillus cereus, escherichia coli, listeria monocytogenes, proteus vulgaris, pseudomonas aeruginosa, pseudomonas fluorescens, salmonella abony and staphylococcus aureus as well as against two yeast st ...	2015	26749799
comparison of three bacillus amyloliquefaciens strains growth behaviour and evaluation of the spoilage risk during bread shelf-life.	this study aims at the characterisation of growth behaviour of three strains of bacillus amyloliquefaciens, isolated from ropy bread (atcc8473), wheat grain (ispa-s109.3) and semolina (ispa-n9.1) to estimate rope spoilage risk in pan bread during shelf-life using the sym'previus tool. cardinal values and growth/no growth boundaries were determined in broth, while artificial spore inoculations were performed in dough for various pan bread recipes to compare experimental counts with in silico grow ...	2015	25481056
immobilization of proteases on chitosan for the development of films with anti-biofilm properties.	bacterial resistance due to biofilm formation-particularly staphylococci biofilms-is associated with multiple problems in medical settings where biofilms can colonize medical indwelling devices and cause nosocomial infections. it was against this backdrop that we explored the anti-biofilm activities of a set of proteases against biofilm formation by staphylococcus aureus, listeria monocytogenes and pseudomonas aeruginosa. the selected screened enzymes were immobilized on chitosan to obtain films ...	2015	25451753
mechanistic aspects of biologically synthesized silver nanoparticles against food- and water-borne microbes.	in the present study, silver nanoparticles (agnps) synthesized from aqueous leaves extract of malva crispa and their mode of interaction with food- and water-borne microbes were investigated. formation of agnps was conformed through uv-vis, fe-sem, eds, afm, and hr-tem analyses. further the concentration of silver (ag) in the reaction mixture was conformed through icp-ms analysis. different concentration of nanoparticles (1-3 mm) tested to know the inhibitory effect of bacterial pathogens such a ...	2015	26178241
composition, antimicrobial, antioxidant, and antiproliferative activity of origanum dictamnus (dittany) essential oil.	nowadays, there has been an increased interest in essential oils from various plant origins as potential antimicrobial, antioxidant, and antiproliferative agents. this trend can be mainly attributed to the rising number and severity of food poisoning outbreaks worldwide along with the recent negative consumer perception against artificial food additives and the demand for novel functional foods with possible health benefits. origanum dictamnus (dittany) is an aromatic, tender perennial plant tha ...	2015	25952773
recent developments in understanding the iron acquisition strategies of gram positive pathogens.	iron is a versatile redox-active catalyst and a required cofactor within a diverse array of biological processes. to almost all organisms, iron is both essential and potentially toxic, where homeostatic concentrations must be stringently maintained. within the iron-restricted host, the survival and proliferation of microbial invaders is contingent upon exploiting the host iron pool. bacteria express a multitude of complex, and often redundant means of acquiring iron, including surface-associated ...	2015	25862688
evaluation of anti-bacterial effects of some novel thiazole and imidazole derivatives against some pathogenic bacteria.	bacterial resistance to antibiotics has motivated the researchers to evaluate the novel anti-bacterial compounds such as some thiazole and imidazole derivatives. thereby, in this work, we investigated the anti-bacterial effects of one new thiazole and two new imidazole derivatives on bacillus cereus, listeria monocytogenes, escherichia coli, salmonella typhimurium, proteus mirabilis and shigella dysenteriae.	2015	26719785
comparison of primary models to predict microbial growth by the plate count and absorbance methods.	the selection of a primary model to describe microbial growth in predictive food microbiology often appears to be subjective. the objective of this research was to check the performance of different mathematical models in predicting growth parameters, both by absorbance and plate count methods. for this purpose, growth curves of three different microorganisms (bacillus cereus, listeria monocytogenes, and escherichia coli) grown under the same conditions, but with different initial concentrations ...	2015	26539483
enteric bacteria isolated from diarrheal patients in korea in 2014.	the aim of this study was to characterize the pathogens responsible for causing diarrhea according to season, region of isolation, patient age, and sex as well as to provide useful data for the prevention of diarrheal disease.	2015	26473090
total phenolic and flavonoid content and antibacterial activity of punica granatum l. var. pleniflora flowers (golnar) against bacterial strains causing foodborne diseases.	flowers of punica granatum l. (punicaceae) var. pleniflora, known as "golnar" in iranian traditional medicine have been used for the prevention and treatment of foodborne diseases. in this study, antibacterial activities of ethanol extract of golnar and its fractions were scientifically evaluated against bacteria causing foodborne diseases including staphylococcus aureus, bacillus cereus, listeria monocytogenes, escherichia coli, shigella dysantriae, and salmonella typhi. the total phenolic and ...	2015	26470879
isolation and characterization of bacteriocinogenic lactic bacteria from m-tuba and tepache, two traditional fermented beverages in méxico.	mexican tuba (m-tuba) and tepache are mexican fermented beverages prepared mainly with pineapple pulp and coconut palm, respectively. at present, reports on the microbiota and nutritional effects of both beverages are lacking. the purpose of this study was to determine whether m-tuba and tepache contain cultivable lactic acid bacteria (lab) capable of producing bacteriocins. tepache and m-tuba contain mesophilic aerobic bacteria, lab, and yeast. bacillus subtilis, listeria monocytogenes, listeri ...	2015	26405529
antibacterial effect and mechanism of high-intensity 405 ± 5 nm light emitting diode on bacillus cereus, listeria monocytogenes, and staphylococcus aureus under refrigerated condition.	this study investigated the antibacterial effect of 405 ± 5 nm light emitting diode (led) on bacillus cereus, listeria monocytogenes, and staphylococcus aureus, and examined its antibacterial mechanism by determining the bacterial membrane and dna damages. a 405 ± 5 nm led illuminated the gram-positive pathogens until 486 j/cm(2) at 4 °c. weibull model was used to calculate reliable life (tr) to compare bacterial sensitivities to led illumination. the membrane damage was determined by nacl and l ...	2015	26398810
chemical composition and in vitro antibacterial activity of mentha spicata essential oil against common food-borne pathogenic bacteria.	the aim of the present study was to investigate chemical composition and antibacterial activity of essential oil from the leaf of mentha spicata plant against common food-borne pathogenic bacteria (staphylococcus aureus, bacillus subtilis, bacillus cereus, listeria monocytogenes, salmonella typhimurium, and escherichia coli o157:h7). chemical composition of the essential oil was identified by gas chromatography coupled with mass spectrometer detector (gc-ms). the antibacterial activity of the es ...	2015	26351584
reduction of listeria monocytogenes and bacillus cereus in milk by zinc oxide nanoparticles.	direct addition of antimicrobial materials to food during food processing is an effective method for controlling microbial contaminants of food and extending the shelf- life of food products. objective of this research was to study the antimicrobial effect of zinc oxide (zno) nanoparticle and potential applications of zno nanoparticles in terms of controling two food-borne pathogens in milk.	2015	26351469
a study on characterization of new bacteriocin produced from a novel strain of lactobacillus spicheri g2 isolated from gundruk- a fermented vegetable product of north east india: a novel bacteriocin production from lactobacillus spicheri g2.	bacteriocin producing lactobacillus spicheri g2, isolated from gundruk - a traditional fermented vegetable product of north east india. l. spicheri g2 identified by morphological, biochemical techniques followed by 16s rrna gene technique. the 16sr rna sequence of bacteriocin producer is registered in ncbi under accession no. jx481912. the bacteriocin producing potential of l. spicheri is being reported for the first time in the present investigation. bacteriocin of l. spicheri g2 showed strong ...	2015	26344995
antibacterial effects and action modes of asiatic acid.	in this study, the antibacterial effects and action modes of asiatic acid against the foodborne bacterial pathogens escherichia coli o157:h7, salmonella typhimurium dt104, pseudomonas aeruginosa, listeria monocytogenes, staphylococcus aureus, enterococcus faecalis, and bacillus cereus were examined. minimal inhibitory concentrations (mics) of asiatic acid against these bacteria were in the range of 20-40 μg/ml. minimum bactericidal concentrations of asiatic acid were in the range of 32-52 μg/ml. ...	2015	26280399
stability, antimicrobial activity, and effect of nisin on the physico-chemical properties of fruit juices.	heat processing is the most commonly used hurdle for inactivating microorganisms in fruit juices. however, this preservation method could interfere with the organoleptic characteristics of the product. alternative methods have been proposed and bacteriocins such as nisin are potential candidates. however, the approval of bacteriocins as food additives is limited, especially in foods from vegetal origin. we aimed to verify the stability, the effect on physico-chemical properties, and the antimicr ...	2015	26162590
evaluation of synergistic antibacterial and antioxidant efficacy of essential oils of spices and herbs in combination.	the present study was carried out to evaluate the possible synergistic interactions on antibacterial and antioxidant efficacy of essential oils of some selected spices and herbs [bay leaf, black pepper, coriander (seed and leaf), cumin, garlic, ginger, mustard, onion and turmeric] in combination. antibacterial combination effect was evaluated against six important food-borne bacteria (bacillus cereus, listeria monocytogenes, micrococcus luteus, staphylococcus aureus, escherichia coli and salmone ...	2015	26132146
antimicrobial mechanism of resveratrol-trans-dihydrodimer produced from peroxidase-catalyzed oxidation of resveratrol.	plant polyphenols are known to have varying antimicrobial potencies, including direct antibacterial activity, synergism with antibiotics and suppression of bacterial virulence. we performed the in vitro oligomerization of resveratrol catalyzed by soybean peroxidase, and the two isomers (resveratrol-trans-dihydrodimer and pallidol) produced were tested for antimicrobial activity. the resveratrol-trans-dihydrodimer displayed antimicrobial activity against the gram-positive bacteria bacillus cereus ...	2015	26109045
a novel suicide plasmid for efficient gene mutation in listeria monocytogenes.	although several plasmids have been used in listeria monocytogenes for generating mutants by allelic exchange, construction of l. monocytogenes mutants has been inefficient due to lack of effective selection markers for first and second recombination events. to address this problem, we have developed a new suicide plasmid, phoss1, by using the pmad plasmid backbone and anhydrotetracycline selection marker (secy antisense rna) driven by an inducible pxyl/teto promoter. expression of the secy anti ...	2015	26038185
kinetics of bacterial inactivation by 405nm and 520nm light emitting diodes and the role of endogenous coproporphyrin on bacterial susceptibility.	photodynamic inactivation studies of microbial pathogens have focused on the use of an external photosensitizer or a precursor compound to eliminate bacteria. the present study investigated the inactivation kinetics of six bacterial pathogens by a 405nm light emitting diode (led) without the addition of any external compound. the role of endogenous coproporphyrin on the bacterial susceptibility to leds was also examined. pathogens were illuminated with leds at 25, 10 and 4°c for 9h and the inact ...	2015	26036659
isolation of lactic acid bacteria showing antioxidative and probiotic activities from kimchi and infant feces.	the purpose of this study was to investigate lactic acid bacteria with antioxidative and probiotic activities isolated from korean healthy infant feces and kimchi. isolates a1, a2, s1, s2, and s3 were assigned to lactobacillus sp. and isolates a3, a4, e1, e2, e3, and e4 were assigned to leuconostoc sp. on the basis of their physiological properties and 16s ribosomal dna sequence analysis. most strains were confirmed as safe bioresources through nonhemolytic activities and non-production of harmf ...	2015	25951843
bactericidal mechanism of bio-oil obtained from fast pyrolysis of pinus densiflora against two foodborne pathogens, bacillus cereus and listeria monocytogenes.	foodborne bacteria are the leading cause of food spoilage and other related diseases. in the present study, the antibacterial activity of bio-oil (bo) manufactured by fast pyrolysis of pinewood sawdust (pinus densiflora siebold and zucc.) against two disease-causing foodborne pathogens (bacillus cereus and listeria monocytogenes) was evaluated. bo at a concentration of 1000 μg/disc was highly active against both b. cereus (10.0-10.6 mm-inhibition zone) and l. monocytogenes (10.6-12.0-mm inhibiti ...	2015	25928035
microbiological diversity and prevalence of spoilage and pathogenic bacteria in commercial fermented alcoholic beverages (beer, fruit wine, refined rice wine, and yakju).	the present study examined 469 commercially available fermented alcoholic beverages (fabs), including beer (draft, microbrewed, and pasteurized), fruit wine (grape and others), refined rice wine, and yakju (raw and pasteurized). samples were screened for escherichia coli and eight foodborne pathogens (bacillus cereus, campylobacter jejuni, clostridium perfringens, escherichia coli o157:h7, listeria monocytogenes, salmonella spp., staphylococcus aureus, and yersinia enterocolitica), and the aerob ...	2015	25836410
expert elicitation as a means to attribute 28 enteric pathogens to foodborne, waterborne, animal contact, and person-to-person transmission routes in canada.	enteric illness contributes to a significant burden of illness in canada and globally. understanding its sources is a critical step in identifying and preventing health risks. expert elicitation is a powerful tool, used previously, to obtain information about enteric illness source attribution where information is difficult or expensive to obtain. thirty-one experts estimated transmission of 28 pathogens via major transmission routes (foodborne, waterborne, animal contact, person-to-person, and ...	2015	25835810
antimicrobial activities of active component isolated from lawsonia inermis leaves and structure-activity relationships of its analogues against food-borne bacteria.	the antimicrobial activities of lawsonia inermis leaf extract and 2-hydroxy-1,4-naphthoquinone analogues against food-borne bacteria. the antimicrobial activities of five fractions derived from the methanol extract of lawsonia inermis leaves were evaluated against 7 food-borne bacteria. 2-hydroxy-1,4-naphthoquinone was isolated by chromatographic analyses. 2-hydroxy-1,4-naphthoquinone showed the strong activities against bacillus cereus, listeria monocytogenes, salmonella enterica, shigella sonn ...	2015	25829631
lactic acid bacteria: promising supplements for enhancing the biological activities of kombucha.	kombucha is sweetened black tea that is fermented by a symbiosis of bacteria and yeast embedded within a cellulose membrane. it is considered a health drink in many countries because it is a rich source of vitamins and may have other health benefits. it has previously been reported that adding lactic acid bacteria (lactobacillus) strains to kombucha can enhance its biological functions, but in that study only lactic acid bacteria isolated from kefir grains were tested. there are many other natur ...	2015	25763303
the length of a lantibiotic hinge region has profound influence on antimicrobial activity and host specificity.	lantibiotics are ribosomally synthesized (methyl)lanthionine containing peptides which can efficiently inhibit the growth of gram-positive bacteria. as lantibiotics kill bacteria efficiently and resistance to them is difficult to be obtained, they have the potential to be used in many applications, e.g., in pharmaceutical industry or food industry. nisin can inhibit the growth of gram-positive bacteria by binding to lipid ii and by making pores in their membrane. the c-terminal part of nisin is ...	2015	25688235
antimicrobial potential of cauliflower, broccoli, and okara byproducts against foodborne bacteria.	the antimicrobial potential of cauliflower, broccoli, and okara byproducts was assessed against gram-positive and gram-negative bacteria. salmonella enterica serovar typhimurium, escherichia coli o157:h7, bacillus cereus, and listeria monocytogenes serovar 4b growth behavior was assessed under exposure to 5% vegetable byproducts added to the reference medium, buffered peptone water (0.1% [wt/vol]), at 37°c. although the byproducts were not effective against l. monocytogenes, they were bactericid ...	2015	25587927
effect of packaging technology on microbiological and sensory quality of a cooked blood sausage, morcela de arroz, from monchique region of portugal.	morcela de arroz (ma), a popular portuguese blood sausage, with high ph and water activity (aw), is traditionally commercialized without preservatives and unpacked. this study evaluated the best packaging solution to extend ma shelf life stored at 4±1°c for 44days: without packaging (wp), vacuum (vp) and modified atmosphere packaging (map) (80% co2; 20% n2). mesophilic (mtvc), psychrotrophic (ptvc), lactic acid bacteria (lab), pseudomonads, molds and yeasts, enterobacteriaceae, listeria monocyto ...	2015	25462380
antibacterial activity and genotypic-phenotypic characteristics of bacteriocin-producing bacillus subtilis kku213: potential as a probiotic strain.	the antimicrobial activity and probiotic properties of bacillus subtilis strain kku213, isolated from local soil, were investigated. the cell-free supernatant (cfs) of a kku213 culture containing crude bacteriocins exhibited inhibitory effects on gram-positive bacteria, including bacillus cereus, listeria monocytogenes, micrococcus luteus, and staphylococcus aureus. the antibacterial activity of the cfs precipitated with 40% ammonium sulfate (as) remained even after treatment at 60 and 100 °c, a ...	2015	25440998
evaluation of haccp system implementation on the quality of mixed fresh-cut salad prepared in a university canteen: a case study.	the increasing awareness that foods can represent vehicles for health risk factors has caused scientists and public authorities to multiply their efforts to reduce these risks to within acceptable limits. nevertheless, some challenging issues still remain unsolved and new ones have recently emerged, such as the increase in outbreaks of foodborne diseases originating from the consumption of meals at catering facilities. the study described in this article was aimed at evaluating the microbiologic ...	2015	25619040
potential probiotic attributes of a new strain of bacillus coagulans cgmcc 9951 isolated from healthy piglet feces.	a new strain of bacillus coagulans cgmcc 9551, which has a broad range of antibacterial activities against six main pathogenic bacteria including escherichia coli o8, staphylococcus aureus, salmonella enterica subsp. enterica serovar enteritidis, streptococcus suis, listeria monocytogenes and pasteurella multocida, was isolated from healthy piglet feces. in adhesion assay, the isolate exhibited a stronger adhesion to pig intestinal mucus than that of b. subtilis jt143 and l. acidophilus ly24 res ...	2015	25752235
to modulate survival under secondary stress conditions, listeria monocytogenes 10403s employs rsbx to downregulate σb activity in the poststress recovery stage or stationary phase.	listeria monocytogenes is a saprophytic bacterium that thrives in diverse environments and causes listeriosis via ingestion of contaminated food. rsbx, a putative sigma b (σ(b)) regulator, is thought to maintain the ready state in the absence of stress and reset the bacterium to the initial state in the poststress stage in bacillus subtilis. we wondered whether rsbx is functional in l. monocytogenes under different stress scenarios. genetic deletion and complementation of the rsbx gene were comb ...	2015	26637594
secondary metabolites constituents and antioxidant, anticancer and antibacterial activities of etlingera elatior (jack) r.m.sm grown in different locations of malaysia.	etlingera elatior is a well-known herb in malaysia with various pharmaceutical properties.	2015	26399961
identification of conserved and species-specific functions of the listeria monocytogenes prsa2 secretion chaperone.	the gram-positive bacterium listeria monocytogenes is a facultative intracellular pathogen that relies on the regulated secretion and activity of a variety of proteins that sustain life within diverse environments. prsa2 has recently been identified as a secreted peptidyl-prolyl cis/trans isomerase and chaperone that is dispensable for bacterial growth in broth culture but essential for l. monocytogenes virulence. following host infection, prsa2 contributes to the proper folding and activity of ...	2015	26216425
label-free identification of individual bacteria using fourier transform light scattering.	rapid identification of bacterial species is crucial in medicine and food hygiene. in order to achieve rapid and label-free identification of bacterial species at the single bacterium level, we propose and experimentally demonstrate an optical method based on fourier transform light scattering (ftls) measurements and statistical classification. for individual rod-shaped bacteria belonging to four bacterial species (listeria monocytogenes, escherichia coli, lactobacillus casei, and bacillus subti ...	2015	26193558
employing carbon dots modified with vancomycin for assaying gram-positive bacteria like staphylococcus aureus.	by employing attractive performance of fluorescent carbon dots, we herein successfully established an assay for analyzing bacteria firstly. specifically, carbon dots with blue fluorescence were initially synthesized according to a previous report, and modified with vancomycin on their surfaces. subsequently, the prepared carbon dots were applied to detect staphylococcus aureus accompanied with a linear range of 3.18×10(5)-1.59×10(8) cfu/ml as well as a detection limit of 9.40×10(4) cfu/ml. compa ...	2015	26188677
biological activities and dna interactions of amanita ovoidea.	amanita ovoidea (bull.) link (amanitaceae) is a well-known species due to its pleasant aroma and flavor since ancient times in the worldwide. this species is also known in turkey and people consume it extensively.	2015	25874387
a jack of all trades: the multiple roles of the unique essential second messenger cyclic di-amp.	second messengers are key components of many signal transduction pathways. in addition to cyclic amp, ppgpp and cyclic di-gmp, many bacteria use also cyclic di-amp as a second messenger. this molecule is synthesized by distinct classes of diadenylate cyclases and degraded by phosphodiesterases. the control of the intracellular c-di-amp pool is very important since both a lack of this molecule and its accumulation can inhibit growth of the bacteria. in many firmicutes, c-di-amp is essential, maki ...	2015	25869574
bacillus subtilis subsp. subtilis cbmdc3f with antimicrobial activity against gram-positive foodborne pathogenic bacteria: uv-maldi-tof ms analysis of its bioactive compounds.	in this work a new bacillus sp. strain, isolated from honey, was characterized phylogenetically. its antibacterial activity against three relevant foodborne pathogenic bacteria was studied; the main bioactive metabolites were analyzed using ultraviolet matrix assisted laser desorption-ionization mass spectrometry (uv-maldi ms). bacillus cbmdc3f was phylogenetically characterized as bacillus subtilis subsp. subtilis after rrna analysis of the 16s subunit and the gyra gene (access codes genbank jx ...	2015	25820813
prospecting russula senecis: a delicacy among the tribes of west bengal.	russula senecis, a worldwide distributed mushroom, is exclusively popular among the tribal communities of west bengal for food purposes. the present study focuses on its reliable taxonomic identification through macro- and micro-morphological features, dna barcoding, confirmation of its systematic placement by phylogenetic analyses, myco-chemicals and functional activities. for the first time, the complete internal transcribed spacer region of r. senecis has been sequenced and its taxonomic posi ...	2015	25780764
metabolic gene expression shift by listeria monocytogenes in coculture biofilms.	coculture communities of microbes are more realistic and common in nature than in laboratory-grown pure cultures. in a mixed community, when resources with a potential role in growth are shared, conflict (as a consequence of competition) or cooperation is certain. in our study, this situation of conflict and cooperation was explored to understand the population dynamics and community behavior of listeria monocytogenes. the social behavioral response of l. monocytogenes to the presence of bacillu ...	2015	25776109
probiotic potential of lactobacillus strains isolated from sorghum-based traditional fermented food.	sorghum-based traditional fermented food was screened for potential probiotic lactic acid bacteria. the isolates were identified by biochemical, physiological and genetic methods. species identification was done by 16s rrna sequence analysis. the functional probiotic potential of the two lactobacillus species viz., lactobacillus plantarum (lact. plantarum) and lactobacillus pentosus (lact. pentosus) was assessed by different standard parameters. the strains were tolerant to ph 2 for 1 h and resi ...	2015	25666113
structural and biochemical analysis of the essential diadenylate cyclase cdaa from listeria monocytogenes.	the recently identified second messenger cyclic di-amp (c-di-amp) is involved in several important cellular processes, such as cell wall metabolism, maintenance of dna integrity, ion transport, transcription regulation, and allosteric regulation of enzyme function. interestingly, c-di-amp is essential for growth of the gram-positive model bacterium bacillus subtilis. although the genome of b. subtilis encodes three c-di-amp-producing diadenlyate cyclases that can functionally replace each other, ...	2015	25605729
production of antibacterial bombyx mori cecropin a in mealworm-pathogenic beauveria bassiana erl1170.	efforts are underway to produce antimicrobial peptides in yellow mealworms (tenebrio molitor), which can be developed as more effective and safer animal feed additives. in this work, we expressed bombyx mori (bm) cecropin-a in mealworms by the infection of transformed entomopathogenic beauveria bassiana erl1170. the active domain of bm cecropin a gene was tagged with a signal sequence of b. bassiana for extracellular secretion, and the fragment was inserted into erl1170 by the restriction enzyme ...	2015	25429897
bioprocess development for the production of sonorensin by bacillus sonorensis mt93 and its application as a food preservative.	media composition and environmental conditions were optimized using statistical tools, plackett burman design and response surface methodology, to maximize the yield of a bacteriocin, named as sonorensin, from a new marine isolate bacillus sonorensis mt93 showing broad spectrum of antimicrobial activity. under optimized conditions, mt93 produced 15-fold higher yield of sonorensin compared to that under initial fermentation conditions. as oxygen supply is a critical parameter controlling growth a ...	2015	25459843
association of histophilus somni with spontaneous abortions in dairy cattle herds from brazil.	this study investigated the participation of infectious agents in spontaneous abortions and reproductive problems at eight dairy cattle herds from three geographical regions of brazil. fourteen aborted fetuses and the organ sections of one cow with history of repeated abortions were received for pathological evaluations and molecular diagnostics. pcr/rt-pcr assays targeted specific genes of abortifacient agents of cattle: bovine viral diarrhea virus (bvdv), bovine herpesvirus 1 (bohv-1), listeri ...	2015	25480485
rapid identification and classification of campylobacter spp. using laser optical scattering technology.	campylobacter jejuni and campylobacter coli are the two important species responsible for most of the campylobacter infections in humans. reliable isolation and detection of campylobacter spp. from food samples are challenging due to the interferences from complex food substances and the fastidious growth requirements of this organism. in this study, a novel biosensor-based detection called bardot (bacterial rapid detection using optical scattering technology) was developed for high-throughput s ...	2015	25583335
[identification of zoonotic bacterial pathogens by the maldi tof ms method].	to verify whether the maldi tof ms method can be used for rapid identification of selected zoonotic bacterial pathogens isolated from various types of materials in the real conditions of routine laboratory work.	2015	26312374
production of bacteriocin-like inhibitory substance by bifidobacterium lactis in skim milk supplemented with additives.	bacteriocins are natural compounds used as food biopreservatives instead of chemical preservatives. bifidobacterium animalis subsp. lactis (bifid. lactis) was shown to produce a bacteriocin-like inhibitory substance (blis) able to inhibit the growth of listeria monocytogenes selected as an indicator microorganism. to enhance this production by the strain bifid. lactis bl 04, skim milk (sm) was used as a fermentation medium either in the presence or in the absence of yeast extract, tween 80 or in ...	2015	25850555
effectiveness of inactivation of foodborne pathogens during simulated home pan frying of steak, hamburger or meat strips.	in order to evaluate the effect of simulated home pan frying of raw meat and meat preparations of different animal species on the thermal inactivation of pathogens, the heat resistance (d-value) of three strains of campylobacter jejuni, escherichia coli o157:h7, salmonella spp., listeria monocytogenes and two strains of generic e. coli was validated in bhi and adjusted bhi (i.e. ph5.6 and 1.5% nacl) at 60°c. the d-values were obtained of the linear phase of the survivor curves created in ginafit ...	2015	26004267
effect of oxygen stress on growth and survival of clostridium perfringens, campylobacter jejuni, and listeria monocytogenes under different storage conditions.	this study investigated the growth and survival of three foodborne pathogens (clostridium perfringens, campylobacter jejuni, and listeria monocytogenes) in beef (7% fat) and nutrient broth under different oxygen levels. samples were tested under anoxic (<0.5%), microoxic (6 to 8%), and oxic (20%) conditions during storage at 7 °c for 14 days and at 22 °c for 5 days. two initial inoculum concentrations were used (1 and 2 log cfu per g of beef or per ml of broth). the results show that c. perfring ...	2015	25836393
antibiotic susceptibility and prevalence of foodborne pathogens in poultry meat in romania.	the occurrence of pathogenic strains in poultry meat is of growing concern in romania. another problem found on a global level is the continuous increase of antimicrobial resistance in bacteria isolated from food. this study aimed to evaluate the prevalence of pathogenic bacteria in poultry carcasses obtained in romania in 2012-2013 and to reveal the most prevalent patterns of antimicrobial resistance in the isolated strains.	2015	25596569