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biological activity, design, synthesis and structure activity relationship of some novel derivatives of curcumin containing sulfonamides.five series of curcumin derivatives with sulfonamides 3a-3e, 4a-4e, 5a-5e, 6a-6e and 7a-7e have been synthesized and evaluated for in vitro antibacterial activity against selected medically important gram-(+) and gram-(-) bacterial species viz. staphylococcus aureus, bacillus cereus, salmonella typhi, pseudomonas aeruginosa and escherichia coli, and antifungal activity against few pathogenic fungal species viz. aspergillus niger, aspergillus flavus, trichoderma viride and curvularia lunata. the ...201323685942
synergized antimicrobial activity of eugenol incorporated polyhydroxybutyrate films against food spoilage microorganisms in conjunction with pediocin.biopolymers and biopreservatives produced by microorganisms play an essential role in food technology. polyhydroxyalkanoates and bacteriocins produced by bacteria are promising components to safeguard the environment and for food preservation applications. polyhydroxybutyrate (phb)-based antimicrobial films were prepared incorporating eugenol, from 10 to 200 μg/g of phb. the films were evaluated for antimicrobial activity against foodborne pathogens, spoilage bacteria, and fungi such as staphylo ...201323666640
design, characterization, teratogenicity testing, antibacterial, antifungal and dna interaction of few high spin fe(ii) schiff base amino acid complexes.in this study, new fe(ii) schiff base amino acid chelates derived from the condensation of o-hydroxynaphthaldehyde with l-alanine, l-phenylalanine, l-aspartic acid, l-histidine and l-arginine were synthesized and characterized via elemental, thermogravimetric analysis, molar conductance, ir, electronic, mass spectra and magnetic moment measurements. the stoichiometry and the stability constants of the complexes were determined spectrophotometrically. correlation of all spectroscopic data suggest ...201323665616
synthesis, antimicrobial, antiquorum-sensing, antitumor and cytotoxic activities of new series of fused [1,3,4]thiadiazoles.new series of [1,3,4]thiadiazolo[3,2-a]pyrimidines, benzo[h][1,3,4]thiadiazolo[2,3-b]quinazolines, benzothiadiazolotriazocine, and imidazo[2,1-b][1,3,4]thiadiazoles have been synthesized and characterized by analytical and spectrometrical methods (ir, ms, (1)h and (13)c nmr). twenty of the synthesized compounds were screened for antibacterial activity against escherichia coli, staphylococcus aureus and bacillus cereus. they were found to be either moderately active, slightly active or inactive a ...201323474904
purification of an antifungal compound, cyclo(l-pro-d-leu) for cereals produced by bacillus cereus subsp. thuringiensis associated with entomopathogenic nematode.mold spoilage is the main cause of substantial economic loss in cereals and might also cause public health problems due to the production of mycotoxins. the aim of this study was to separate and purify and to identify antifungal compounds of bacterium associated with novel entomopathogenic nematode and check the antifungal property of identified compound in particular food model systems. the antifungal compound was purified using silica gel column chromatography, tlc and hplc and its structure w ...201323305769
plant-based hydrocarbon esters from tragia involucrata possess antimicrobial and anti-inflammatory activities.antimicrobial and anti-inflammatory activities of hydrocarbon esters obtained from tragia involucrata were evaluated by disk-diffusion (250 µg/ml), and broth-dilution (500-7.8 µg/ml), methods against bacteria. among the compounds, shellsol showed the most potent activity against burkholderia pseudomallei (khw), aeromonas hydrophila, staphylococcus aureus, bacillus subtilis, streptococcus pyogenes, klebsiella pneumoniae, proteus mirabilis, and streptococcus pneumoniae. interestingly, vinyl hexyle ...201323713670
[synthesis and antimicrobial ivestigation of 8-quinolinols glucosaminides].in the phase transfer system solid calcium carbonate--acetonitrile per acetylated o-beta-d-glucosaminides of 8-quinolinol, 2-methyl-8-quinolinol and 5-chloro-8-quinolinol were synthesized. zemplen deacetylation was led to corresponding trioles. obtained o-beta-d-glucosaminides were identified with 1h-nmr spectroscopy. antimicrobial activity of deacetylated forms has been studied in a test of inhibition of bioluminescence of marine luminescent bacteria vibrio fischeri f1 and method of the serial ...201323189563
secondary metabolites: applications on cultural heritage.biological sciences and related bio-technology play a very important role in research projects concerning protection and preservation of cultural heritage for future generations. in this work secondary metabolites of burkholderia gladioli pv. agaricicola (bga) icmp 11096 strain and crude extract of glycoalkaloids from solanaceae plants, were tested against a panel of microorganisms isolated from calcarenite stones of two historical bridges located in potenza and in campomaggiore (southern italy) ...201325145230
chemical composition and biological activity of the essential oil from leaves of moringa oleifera lam. cultivated in mozambique.the antioxidant capacity and antimicrobial activity of the essential oil of moringa oleifera (moringaceae) grown in mozambique was investigated. the chemical composition was studied by means of gc and gc-ms analysis. hexacosane (13.9%), pentacosane (13.3%) and heptacosane (11.4%) were the main components. ultra high performance chromatography-dad analysis detected the flavonoids quercetin (126 μg/g) and luteolin (6.2 μg/g). the essential oil exhibited a relatively low free radical scavenging cap ...201324022760
volatile constituents of pinus roxburghii from nepal.pinus roxburghii sarg. is one of 3 species of pine found in nepal, the oil of which is traditionally used to treat cuts, wounds, boils, and blisters.201323598924
antimicrobial activity of a new preservative for multiuse ophthalmic solutions.the aim of this study was to examine the antimicrobial activity and the preservative efficacy of a novel preservative solution containing sodium hydroxymethyl glycinate (shmg) and edetate disodium (edta), which is used for preservation of some commercial ophthalmic formulations.201323428175
copper(ii) triflate promoted multicomponent catalytic clubbing of piperazinyl-thiazoloquinolines and thiazolocoumarins as antimicrobials.step economical, multicomponent construction of two series based on quinoline and coumarin ring systems was carried out. annulation towards the 2-piperazinylthiazole system was established in satisfactory yields, using copper(ii) triflate. these multicomponent reaction products were examined for their potential inhibitory effect against two gram-positive bacteria (staphylococcus aureus and bacillus cereus), three gram-negative bacteria (escherichia coli, pseudomonas aeruginosa, and klebsiella pn ...201323423648
influence of substituent effects on spectroscopic properties and antimicrobial activity of 5-(4'-substituted phenylazo)-2-thioxothiazolidinone derivatives.5-(4'-substituted phenylazo)-2-thioxothiazolidinone derivatives (hl(n)) have been synthesized and characterized by elemental analysis, spectra (ir, electronic and (1)h nmr). the ir spectral data indicate that the compounds can exist in two resonance structures. the synthesized ligands were screened for their antimicrobial activity against four bacterial species, two gram positive bacteria (bacillus cereus and staphylococcus aureus) and two gram negative bacteria (escherichia coli and klebsiella ...201322968655
synthesis of a new class of antimicrobial agents incorporating the indolin-2-one moiety.new furanone derivatives incorporating the indolin-2-one moiety 3 were prepared via the perkin reaction of isatins 1 with aroylpropionic acids 2 under conventional conditions or microwave irradiation. a series of functionally heterocyclic derivatives (e.g., pyridazines, pyrroles, and sulfonamides) incorporating the indolin-2-one moiety was achieved via reaction of 3 with different reagents under microwave irradiation conditions. the newly synthesized compounds were characterized on the basis of ...201322651801
characterization of amylolysin, a novel lantibiotic from bacillus amyloliquefaciens ga1.lantibiotics are heat-stable peptides characterized by the presence of thioether amino acid lanthionine and methyllanthionine. they are capable to inhibit the growth of gram-positive bacteria, including listeria monocytogenes, staphylococcus aureus or bacillus cereus, the causative agents of food-borne diseases or nosocomial infections. lantibiotic biosynthetic machinery is encoded by gene cluster composed by a structural gene that codes for a pre-lantibiotic peptide and other genes involved in ...201324349428
use of silkworms to evaluate the pathogenicity of bacteria attached to cedar pollen.injection of a japanese cedar pollen suspension into silkworm hemolymph kills the silkworms. a certain species of bacteria proliferated in the hemolymph of the dead silkworms. a 16s rdna analysis demonstrated that the proliferating bacteria were bacillus cereus, bacillus thuringiensis, bacillus weihenstephanensis, and bacillus amyloliquefaciens. among them, b. cereus, b. thuringiensis, and b. weihenstephanensis exhibited hemolysis against sheep red blood cells and were lethal to mice. a culture ...201324071577
characterization of aerobic spore-forming bacteria associated with industrial dairy processing environments and product spoilage.due to changes in the design of industrial food processing and increasing international trade, highly thermoresistant spore-forming bacteria are an emerging problem in food production. minimally processed foods and products with extended shelf life, such as milk products, are at special risk for contamination and subsequent product damages, but information about origin and food quality related properties of highly heat-resistant spore-formers is still limited. therefore, the aim of this study wa ...201323973839
bacillus amyloliquefaciens ssp. plantarum strains as potential protective starter cultures for the production of bikalga, an alkaline fermented food.to identify and screen dominant bacillus spp. strains isolated from bikalga, fermented seeds of hibiscus sabdariffa for their antimicrobial activities in brain heart infusion (bhi) medium and in a h. sabdariffa seed-based medium. further, to characterize the antimicrobial substances produced.201323565829
characterisation and profiling of bacillus subtilis, bacillus cereus and bacillus licheniformis by maldi-tof mass fingerprinting.the bacillus genus includes species such as bacillus cereus, bacillus licheniformis and bacillus subtilis, some of which may be pathogenic or causative agents in the spoilage of food products. the main goal of this work was to apply matrix-assisted laser desorption ionisation-time of flight (maldi-tof) mass fingerprinting to the classification of these bacillus species. genetic analyses were also compared to phyloproteomic analyses. a collection of 57 bacillus strains isolated from fresh and pro ...201323200657
bacillus cereus sensu lato genomes: basis for identifying anthrax disease strain sources. 201324092785
whole-genome sequences of 94 environmental isolates of bacillus cereus sensu lato.bacillus cereus sensu lato is a species complex that includes the anthrax pathogen bacillus anthracis and other bacterial species of medical, industrial, and ecological importance. their phenotypes of interest are typically linked to large plasmids that are closely related to the anthrax plasmids pxo1 and pxo2. here, we present the draft genome sequences of 94 isolates of b. cereus sensu lato, which were chosen for their plasmid content and environmental origins.201324092776
bacillus anthracis diagnostic detection and rapid antibiotic susceptibility determination using 'bioluminescent' reporter phage.genetically modified phages have the potential to detect pathogenic bacteria from clinical, environmental, or food-related sources. herein we assess an engineered 'bioluminescent' reporter phage (wß::luxab) as a clinical diagnostic tool for bacillus anthracis, the etiological agent of anthrax. wß::luxab is able to rapidly (within minutes) detect a panel of b. anthracis strains by transducing a bioluminescent phenotype. the reporter phage displays species specificity by its inability, or signific ...201323994352
differentiation of strains from the bacillus cereus group by rflp-pfge genomic fingerprinting.bacillus mycoides, bacillus pseudomycoides, bacillus weihenstephanensis, bacillus anthracis, bacillus thuringiensis, and bacillus cereus belong to the b. cereus group. the last three species are characterized by different phenotype features and pathogenicity spectrum, but it has been shown that these species are genetically closely related. the macrorestriction analysis of the genomic dna with the noti enzyme was used to generate polymorphism of restriction profiles for 39 food-borne isolates (b ...201323893780
characterization of amiba2446, a novel bacteriolytic enzyme active against bacillus species.there continues to be a need for developing efficient and environmentally friendly treatments for bacillus anthracis, the causative agent of anthrax. one emerging approach for inactivation of vegetative b. anthracis is the use of bacteriophage endolysins or lytic enzymes encoded by bacterial genomes (autolysins) with highly evolved specificity toward bacterium-specific peptidoglycan cell walls. in this work, we performed in silico analysis of the genome of bacillus anthracis strain ames, using a ...201323872558
razor ex anthrax air detection system for detection of bacillus anthracis spores from aerosol collection samples: collaborative study.the razor ex anthrax air detection system was validated in a collaborative study for the detection of bacillus anthracis in aerosol collection buffer. phosphate-buffered saline was charged with 1 mg/ml standardized dust to simulate an authentic aerosol collection sample. the dust-charged buffer was spiked with either b. anthracis ames at 2000 spores/ml or bacillus cereus at 20 000 spores/ml. twelve collaborators participated in the study, with four collaborators at each of three sites. each coll ...201323767365
cross-functionalities of bacillus deacetylases involved in bacillithiol biosynthesis and bacillithiol-s-conjugate detoxification pathways.bshb, a key enzyme in bacillithiol biosynthesis, hydrolyses the acetyl group from n-acetylglucosamine malate to generate glucosamine malate. in bacillus anthracis, ba1557 has been identified as the n-acetylglucosamine malate deacetylase (bshb); however, a high content of bacillithiol (~70%) was still observed in the b. anthracis ∆ba1557 strain. genomic analysis led to the proposal that another deacetylase could exhibit cross-functionality in bacillithiol biosynthesis. in the present study, ba155 ...201323758290
differentiation of bacillus anthracis, b. cereus, and b. thuringiensis on the basis of the csab gene reflects host source.csab gene analysis clustered 198 strains of bacillus anthracis, bacillus cereus, and bacillus thuringiensis into two groups related to mammalian and insect hosts, respectively. mammal-related group i strains also have more s-layer homology (slh) protein genes than group ii strains. this indicates that csab-based differentiation reflects selective pressure from animal hosts.201323563945
bacillus weihenstephanensis characteristics are present in bacillus cereus and bacillus mycoides strains.the bacillus cereus group comprises seven bacterial species: bacillus cereus, bacillus anthracis, bacillus thuringiensis, bacillus mycoides, bacillus pseudomycoides, bacillus cytotoxicus, and bacillus weihenstephanensis. bacillus weihenstephanensis is distinguished based on its capability to grow at 7 °c but not at 43 °c, and the presence of specific signature sequences in the 16s rrna and cspa genes and in several housekeeping genes: glpf, gmk, purh, and tpi. bacillus weihenstephanensis-specifi ...201323413955
vaccine protection against bacillus cereus-mediated respiratory anthrax-like disease in mice.bacillus cereus strains harboring a pxo1-like virulence plasmid cause respiratory anthrax-like disease in humans, particularly in welders. we developed mouse models for intraperitoneal as well as aerosol challenge with spores of b. cereus g9241, harboring pbcxo1 and pbc218 virulence plasmids. compared to wild-type b. cereus g9241, spores with a deletion of the pbcxo1-carried protective antigen gene (paga1) were severely attenuated, whereas spores with a deletion of the pbc218-carried protective ...201323319564
study of charge distribution on the surface of biocolloids.potentiometric titration and zeta potential measurements are crucial techniques for the characterization of the surface properties of bacterial cells. in this study, we investigated the effects of two commonly used electrolytes, nano(3) and naclo(4), on the viability and acid-base properties of gram-positive bacteria bacillus subtilis. b. subtilis are non-pathogenic bacteria which are often used to model the surface properties of pathogenic microorganisms of the same genus, including bacillus an ...201323298596
bacillus cereus g9241 s-layer assembly contributes to the pathogenesis of anthrax-like disease in mice.bacillus cereus g9241, the causative agent of anthrax-like disease, harbors virulence plasmids encoding anthrax toxins as well as hyaluronic acid (ha) and b. cereus exopolysaccharide (bps) capsules. b. cereus g9241 also harbors s-layer genes, including homologs of bacillus anthracis surface array protein (sap), extractable antigen 1 (ea1), and the s-layer-associated proteins (bsls). in b. anthracis, s-layer proteins and bsls attach via their s-layer homology domains (slh) to the secondary cell w ...201323204457
role of mechanical vs. chemical action in the removal of adherent bacillus spores during cip procedures.this study was designed to evaluate the respective roles of mechanical and chemical effects on the removal of bacillus spores during cleaning-in-place. this analysis was performed on 12 strains belonging to the bacillus cereus group (b. cereus, bacillus anthracis, bacillus thuringiensis) or to less related bacillus species (bacillus pumilus, bacillus licheniformis, bacillus sporothermodurans, bacillus subtilis). adherent spores were subjected to rinsing-in-place (mechanical action) and cleaning- ...201323200646
perhydrolase-nanotube paint composites with sporicidal and antiviral activity.act (perhydrolase) containing paint composites were prepared leading to broad-spectrum decontamination. act was immobilized onto multi-walled carbon nanotubes (mwnts) and then incorporated into latex-based paints to form catalytic coatings. these act-based paint composites showed a 6-log reduction in the viability of spores of bacillus cereus and bacillus anthracis (sterne) within 60 min. the paint composites also showed >4-log reduction in the titer of influenza virus (x-31) within 10 min (init ...201323188457
immunization of mice with formalin-inactivated spores from avirulent bacillus cereus strains provides significant protection from challenge with bacillus anthracis ames.bacillus anthracis spores are the infectious form of the organism for humans and animals. however, the approved human vaccine in the united states is derived from a vegetative culture filtrate of a toxigenic, nonencapsulated b. anthracis strain that primarily contains protective antigen (pa). immunization of mice with purified spore proteins and formalin-inactivated spores (fis) from a nonencapsulated, nontoxigenic b. anthracis strain confers protection against b. anthracis challenge when pa is ...201323114705
multilocus sequence analysis of bacillus thuringiensis serovars navarrensis, bolivia and vazensis and bacillus weihenstephanensis reveals a common phylogeny.the bacillus cereus group sensu lato includes six closely-related bacterial species: bacillus cereus, bacillus anthracis, bacillus thuringiensis, bacillus mycoides, bacillus pseudomycoides and bacillus weihenstephanensis. b. thuringiensis is distinguished from the other species mainly by the appearance of an inclusion body upon sporulation. b. weihenstephanensis is distinguished based on its psychrotolerance and the presence of specific signature sequences in the 16s rrna gene and cspa genes. a ...201323073664
molecular signatures for bacillus species: demarcation of the bacillus subtilis and bacillus cereus clades in molecular terms and proposal to limit the placement of new species into the genus bacillus.the genus bacillus is a phylogenetically incoherent taxon with members of the group lacking a common evolutionary history. comprising aerobic and anaerobic spore-forming bacteria, no characteristics are known that can distinguish species of this genus from other similar endospore-forming genera. with the availability of complete genomic data from over 30 different species from this group, we have constructed detailed phylogenetic trees to determine the relationships among bacillus and other clos ...201323475340
discrimination between mesophilic and psychrotolerant strains in the bacillus cereus group based on the psti digestion of the pyca gene.a simple and rapid assay for the detection of bacillus weihenstephanensis isolates and other psychrotolerant strains in the bacillus cereus group was developed. it is based on the presence of a nucleotide substitution at position 795 on the housekeeping pyca gene in all b. weihenstephanensis strains. this mutation creates a psti recognition site. it is absent in mesophilic strains in the b. cereus group. the pyca gene is amplified by pcr and the amplicons submitted to psti digestions. in mesophi ...201323475137
biosorption of cd(ii) by live and dead cells of bacillus cereus rc-1 isolated from cadmium-contaminated soil.the present study investigated the biosorption capacity of live and dead cells of bacillus cereus rc-1 for cd(ii). the biosorption characteristics were investigated as a function of initial ph, contact time, and initial cadmium concentration. equilibrium biosorption was modeled using langmuir, freundlich and redlich-peterson isotherm equations. it was found that the maximum biosorption capacities calculated from langmuir isotherm were 31.95 mg/g and 24.01 mg/g for dead cells and live cells, resp ...201323466537
co-production of surfactin and a novel bacteriocin by bacillus subtilis subsp. subtilis h4 isolated from bikalga, an african alkaline hibiscus sabdariffa seed fermented condiment.bikalga is a hibiscus sabdariffa seed fermented condiment widely consumed in burkina faso and neighboring countries. the fermentation is dominated by bacillus subtilis group species. ten b. subtilis subsp. subtilis (six isolates) and bacillus licheniformis (four isolates) isolated from traditional bikalga were examined for their antimicrobial activity against a panel of 36 indicator organisms including gram-positive and gram-negative bacteria and yeasts. the bacillus spp. isolates showed variabl ...201323466466
airborne microbial composition in a high-throughput poultry slaughtering facility.a high-throughput chicken slaughtering facility in beijing was systematically investigated for numbers of airborne microorganisms. samples were assessed for counts of aerobic bacteria, staphylococcus aureus, total coliforms, escherichia coli, pseudomonas aeruginosa, listeria monocytogenes, bacillus cereus, and salmonella. during a 4-month period (september to december 2011), samples were collected for 10 min three times daily (preproduction, production, and postproduction). samples were collecte ...201323462077
screening of solvent dependent antibacterial activity of prunus domestica.fruit of prunus domestica was extracted in ethanol. the ethanol extract was further extracted with two solvents ethyl acetate and chloroform. the crude ethanol extract and two fractions (ethyl acetate and chloroform) were screened for their antibacterial activity using the agar well diffusion method .they were tested against nine bacteria; five gram positive bacteria (staphylococcus aureus, streptococcuc intermedius, bacillus cereus, bacillus pumilus) and four gram negative bacteria (eschrichia ...201323455215
the significance of genome-based diagnosis for the bacillus cereus species. 201323448787
about the bacillus cereus group. 201323448786
microbial contamination associated with consumption and the growth in plastic bottled beverage.plastic bottles enable the storage of unfinished beverages, and most of microbial contamination has occurred in the unfinished beverage that was left. therefore, we investigated microorganisms in various beverages contaminated by pouring and drinking directly by mouth from the bottle, and analyzed the growth of microorganisms in the beverages at room temperature. in the pouring test, microbial growth was detected in 60 of 320 samples, and 13 bacterial strains, 49 mold strains, and 8 yeast strain ...201323445421
gangrenous mastitis caused by bacillus species in six goats.6 lactating dairy goats were examined because of acute mastitis.201323445297
antimicrobial activity of essential oils from mediterranean aromatic plants against several foodborne and spoilage bacteria.the antimicrobial activity of essential oils extracted from a variety of aromatic plants, often used in the portuguese gastronomy was studied in vitro by the agar diffusion method. the essential oils of thyme, oregano, rosemary, verbena, basil, peppermint, pennyroyal and mint were tested against gram-positive (listeria monocytogenes, clostridium perfringens, bacillus cereus, staphylococcus aureus, enterococcus faecium, enterococcus faecalis, and staphylococcus epidermidis) and gram-negative stra ...201323444311
bacillus "next generation" diagnostics: moving from detection toward subtyping and risk-related strain profiling.the highly heterogeneous genus bacillus comprises the largest species group of endospore forming bacteria. because of their ubiquitous nature, bacillus spores can enter food production at several stages resulting in significant economic losses and posing a potential risk to consumers due the capacity of certain bacillus strains for toxin production. in the past, food microbiological diagnostics was focused on the determination of species using conventional culture-based methods, which are still ...201323440299
sterility testing of apheresis hematopoietic progenitor cell products using an automated blood culture system.aabb standards require monitoring of hematopoietic progenitor cell (hpc) products for microbial contamination. to date, there is no automated blood culture system cleared by the food and drug administration for this application. our objective was to validate the versatrek system (trek diagnostic systems) for sterility testing of apheresis hpc products.201323438051
a probability model for enterotoxin production of bacillus cereus as a function of ph and temperature.bacillus cereus is frequently isolated from a variety of foods, including vegetables, dairy products, meats, and other raw and processed foods. the bacterium is capable of producing an enterotoxin and emetic toxin that can cause severe nausea, vomiting, and diarrhea. the objectives of this study were to assess and model the probability of enterotoxin production of b. cereus in a broth model as affected by the broth ph and storage temperature. a three-strain mixture of b. cereus was inoculated in ...201323433386
microbiological quality and risk factors related to sandwiches served in bakeries, cafés, and sandwich bars in south korea.the objective of this study was to investigate the microbiological quality of sandwiches produced on site and served in bakeries, cafés, and sandwich bars in south korea and to determine the major risk factors affecting the sanitation level in each store (n = 1,120). the microbiological quality of the sandwiches was analyzed, and the sanitation level of each store was evaluated as satisfactory or unsatisfactory based on sanitation guidelines. total coliforms were detected in 906 samples (80.9%), ...201323433370
bacillus cereus fasciitis: a unique pathogen and clinically challenging sequela of inoculation.bacillus cereus is an aerobic, spore-forming, gram-positive rod. it has historically been associated with "fried rice syndrome," a foodborne diarrheal and emetic illness resulting from eating fried rice dishes that have been sitting at room temperature for hours. we report the case of a 9-year-old boy who developed culture-positive b cereus fasciitis of the right lower extremity after being impaled on a tree branch. this case report further elucidates and emphasizes the importance of recognizing ...201323431539
a nanoporous membrane-based impedimetric immunosensor for label-free detection of pathogenic bacteria in whole milk.we introduce a nanoporous membrane based impedimetric immunosensor for the label-free detection of bacterial pathogens in whole milk. a simple and rapid method to modify a commercially available alumina nanoporous membrane with hyaluronic acid (ha) effectively reduced the non-specific binding of biomolecules and other cells, and permitted successful immobilization of antibodies. escherichia coli o157:h7, one of the most harmful food-borne pathogenic bacteria, was tested as a model pathogen in th ...201323428735
antiradical, antimicrobial and cytotoxic activities of commercial beetroot pomace.the by-product of food processing is often utilized as feed, and for the preparation of dietary fiber and biofuel. however, these products are also promising sources of bioactive antioxidants and color giving compounds, which could be used as additives in the food, pharmaceutical and cosmetic industry. the aim of this study was to investigate the phytochemical profile, and the antiradical, antimicrobial and cytotoxic activities of industrial beetroot pomace extract (bpe). the content of phenolic ...201323423147
glucose 6p binds and activates hlyiir to repress bacillus cereus haemolysin hlyii gene expression.bacillus cereus is a gram-positive spore-forming bacterium causing food poisoning and serious opportunistic infections. these infections are characterized by bacterial accumulation despite the recruitment of phagocytic cells. we have previously shown that b. cereus haemolysin ii (hlyii) induces macrophage cell death by apoptosis. in this work, we investigated the regulation of the hlyii gene. we show that hlyiir, the negative regulator of hlyii expression in b. cereus, is especially active durin ...201323405113
concerted action of sphingomyelinase and non-hemolytic enterotoxin in pathogenic bacillus cereus.bacillus cereus causes food poisoning and serious non-gastrointestinal-tract infections. non-hemolytic enterotoxin (nhe), which is present in most b. cereus strains, is considered to be one of the main virulence factors. however, a b. cereus δnhebc mutant strain lacking nhe is still cytotoxic to intestinal epithelial cells. in a screen for additional cytotoxic factors using an in vitro model for polarized colon epithelial cells we identified b. cereus sphingomyelinase (smase) as a strong inducer ...201323613846
identification of tet45, a tetracycline efflux pump, from a poultry-litter-exposed soil isolate and persistence of tet(45) in the soil.to characterize a tetracycline resistance (tc(r)) determinant, closely related to the tetl efflux pump, in a bhargavaea cecembensis strain previously isolated from a poultry-litter-impacted soil.201323595824
evaluation of the haccp system in a university canteen: microbiological monitoring and internal auditing as verification tools.food safety is essential in mass catering. in europe, regulation (ec) no. 852/2004 requires food business operators to put in place, implement and maintain permanent procedures based on hazard analysis and critical control point (haccp) principles. each haccp plan is specifically implemented for the processing plant and processing methods and requires a systematic collection of data on the incidence, elimination, prevention, and reduction of risks. in this five-year-study, the effectiveness of t ...201323594937
reactive oxygen species and the bacteriostatic and bactericidal effects of isoconazole nitrate.bacterial superinfections often occur in dermatomycoses, resulting in greatly inflamed or eczematous skin. the objective of this study was to evaluate the antibacterial efficacy of isoconazole nitrate (isn), a broad-spectrum antimicrobial imidazole, commonly used to treat dermatomycoses. several gram-positive bacteria minimal inhibitory concentrations (mics) for isn (isn solution or isn-containing creams: travogen or corticosteroid-containing travocort) and ampicillin were obtained using the bro ...201323574020
foodborne disease outbreaks caused by bacillus cereus, clostridium perfringens, and staphylococcus aureus--united states, 1998-2008.from 1998 to 2008, 1229 foodborne outbreaks caused by bacillus cereus, clostridium perfringens, and staphylococcus aureus were reported in the united states; 39% were reported with a confirmed etiology. vomiting was commonly reported in b. cereus (median, 75% of cases) and s. aureus outbreaks (median, 87%), but rarely in c. perfringens outbreaks (median, 9%). meat or poultry dishes were commonly implicated in c. perfringens (63%) and s. aureus (55%) outbreaks, and rice dishes were commonly impli ...201323592829
optimization of cultural conditions using response surface methodology versus artificial neural network and modeling of l-glutaminase production by bacillus cereus mtcc 1305.response surface methodology and artificial neural network were used to optimize cultural conditions of l-glutaminase production from bacillus cereus mtcc 1305. ann model was superior to rsm model with higher value of coefficient of determination (99.97ann>97.78rsm), predicted distribution coefficient (0.9992ann>0.896rsm) and lower value of absolute average deviation (1.17%ann<18.47%rsm). optimum cultural conditions predicted by ann were ph (7.5), fermentation time (40 h), temperature (34°c), in ...201323587828
kinetics of bacillus cereus spore inactivation in cooked rice by combined pressure-heat treatment.the efficacy of pressure-heat treatment was evaluated for the inactivation of bacillus cereus spores in cooked rice. the spores of b. cereus atcc 9818 were inoculated (1.1 × 10(8) cfu/g) in a parboiled rice product (ph 6.0, water activity of 0.95) and inactivated to an undetectable level (<10 cfu/g) by treatment of 600 mpa and process temperatures of 60 to 85 °c or 0.1 mpa and 85 °c. kinetic inactivation parameters were estimated with linear and nonlinear models. the potential recovery of injure ...201323575123
antibacterial activity-guided purification and identification of a novel c-20 oxygenated ent-kaurane from rabdosia serra (maxim.) hara.the objective of this work was to conduct an activity-guided isolation of antibacterial compounds from rabdosia serra. the ethanol extracts of r. serra leaf and stem were partitioned sequentially into petroleum ether, ethyl acetate, butanol and water fractions, respectively. the ethanol extract of leaf evidenced broad-spectrum antibacterial activity against gram-positive bacterial, including bacillus subtilis, bacillus cereus, staphylococcus aureus, and listeria monocytogenes. the ethyl acetate ...201323561188
synthesis of amino acid conjugates of tetrahydrocurcumin and evaluation of their antibacterial and anti-mutagenic properties.tetrahydrocurcumin (thc), the hydrogenated and stable form of curcumin, exhibits physiological and pharmacological activities similar to curcumin. a protocol has been developed for the synthesis of novel conjugates of thc with alanine (2a), isoleucine (2b), proline (2c), valine (2d), phenylalanine (2e), glycine (2f) and leucine (2g) in high yields (43-82%). all the derivatives of thc exhibited more potent anti-microbial activity than thc against bacillus cereus, staphylococcus aureus, escherichi ...201323561114
genetic diversity of bacillus thuringiensis from different geo-ecological regions of ukraine by analyzing the 16s rrna and gyrb genes and by ap-pcr and saaflp.the bacillus cereus group consists of closely related species of bacteria and is of interest to researchers due to its importance in industry and medicine. however, it remains difficult to distinguish these bacteria at the intra- and inter-species level. bacillus thuringiensis (bt) is a member of the b. cereus group. in this work, we studied the inter-species structure of five entomopathogenic strains and 20 isolates of bt, which were collected from different geo-ecological regions of ukraine, u ...201323556134
microbial diversity and prevalence of foodborne pathogens in cheap and junk foods consumed by primary schoolchildren.aerobic plate counts (apc), coliforms, bacillus cereus, escherichia coli and eight foodborne pathogens were tested in 1008 cheap and junk foods, including candies, dried cakes, chewing gum, chocolate, dried and seasoned seafood, ice cream, and sugary foods. apcs were positive for 342 samples (33·9%), and the majority of the counts were 2-3 log cfu g(-1) or ml(-1) (average: 1·10 log cfu g(-1) or ml(-1) ). most samples (97·3%) contained no coliforms (average: 0·07 log cfu g(-1) or ml(-1) ). bacill ...201323551123
bacillus cereus. 201323547067
germination and proliferation of emetic bacillus cereus sensu lato strains in milk.the bacillus cereus sensu lato group includes potentially pathogenic bacteria that are ubiquitous in the environment and, importantly, could also be present in food products. this study focuses on emetic isolates which presumably could cause acute food poisoning and emetic syndrome. here, we evaluate the ability of psychrotolerant bacillus weihenstephanensis mc118 (isolated from soil) and mesophilic b. cereus bod3/9 isolated from milk to germinate and multiply at 7 and 30 °c. whereas the rates o ...201323546831
activity and regulation of various forms of cwlj, sleb, and ypeb proteins in degrading cortex peptidoglycan of spores of bacillus species in vitro and during spore germination.germination of bacillus spores requires degradation of a modified layer of peptidoglycan (pg) termed the spore cortex by two redundant cortex-lytic enzymes (cles), cwlj and sleb, plus sleb's partner protein, ypeb. in this study, in vitro and in vivo analyses have been used to clarify the roles of individual sleb and ypeb domains in pg degradation. purified mature bacillus cereus sleb without its signal sequence (sleb(m)) and the sleb c-terminal catalytic domain (sleb(c)) efficiently triggered ge ...201323543708
microbiological examination of ready-to-eat foods and ready-to-bake frozen pastries from university canteens.during a 10-year inspection survey (2001-2010), a microbiological study of ready-to-eat (rte) foods and ready-to-bake frozen pastries from 15 canteens of the university campus was undertaken to determine their microbiological quality. the cumulative study revealed that the aerobic colony counts for the rte product groups were as follows: from 10(6) to 10(8) cfu/g for 50% of sandwiches; under the detection limit (<10 cfu/g) for 88.6% of oven baked pastries; <10(5) cfu/g for 86.5% of desserts oven ...201323541200
in vitro assessment of functional properties of lactic acid bacteria isolated from faecal microbiota of healthy dogs for potential use as probiotics.lactic acid bacteria were isolated and identified in the faeces of chinese crested and yorkshire terrier pups and their probiotic features were investigated in vitro. thirty seven isolates were identified as lactobacillus or enterococcus. out of these isolates, 31 were lactic acid bacteria (lab) and belonged to the species lactobacillus reuteri (16/37; 43.3%), lactobacillus animalis (7/37; 18.9%), lactobacillus acidophilus (3/37; 8.1%), lactobacillus sanfranciscensis (2/37; 5.4%), lactobacillus ...201323538205
pathogenic psychrotolerant sporeformers: an emerging challenge for low-temperature storage of minimally processed foods.sporeforming bacteria are a significant problem in the food industry as they are ubiquitous in nature and capable of resisting inactivation by heat and chemical treatments designed to inactivate them. beyond spoilage issues, psychrotolerant sporeformers are becoming increasingly recognized as a potential hazard given the ever-expanding demand for refrigerated processed foods with extended shelf-life. in these products, the sporeforming pathogens of concern are bacillus cereus, bacillus weihenste ...201323536982
synthesis of 1-isopropyl-3-acyl-5-methyl-benzimidazolone derivatives and their antimicrobial activity.a series of n-acylated analogues of 1-isopropyl-3-acyl-5-methyl-benzimidazolone were synthesized. bioassay results indicated that analogues 5-07 and 5-19 exhibited the most potency against bacillus cereus, bacillus subtilis, staphylococcus aureus, escherichia coli and pseudomonas aeruginosa. analogues 5-02, 5-07, 5-12, 5-15, 5-19, 5-20 and 5-25 could effectively inhibit the spore germination of botrytis cinerea. the relationship between structure and their antimicrobial activity (sar) has also b ...201323531538
potato crop as a source of emetic bacillus cereus and cereulide-induced mammalian cell toxicity.bacillus cereus, aseptically isolated from potato tubers, were screened for cereulide production and for toxicity on human and other mammalian cells. the cereulide-producing isolates grew slowly, the colonies remained small (~1 mm), tested negative for starch hydrolysis, and varied in productivity from 1 to 100 ng of cereulide mg (wet weight)(-1) (~0.01 to 1 ng per 10(5) cfu). by dna-fingerprint analysis, the isolates matched b. cereus f5881/94, connected to human food-borne illness, but were di ...201323524678
microbial contamination of hospital reusable cleaning towels.hospital cleaning practices are critical to the prevention of nosocomial infection transmission. to this end, cloth towels soaked in disinfectants are commonly used to clean and disinfect hospital surfaces. cloth cleaning towels have been linked to an outbreak of bacillus cereus and have been shown to reduce the effectiveness of commonly used quaternary ammonium disinfectants. thus, it is important to determine whether the reuse of cloth towels increases the risk of pathogen transmission in hosp ...201323523522
application of asian pumpkin (cucurbita ficifolia) serine proteinase for production of biologically active peptides from casein.the main objective of this study was to determine potential application of a serine proteinase derived from asian pumpkin for obtaining biologically active peptides from casein. the course of casein hydrolysis by three doses of the enzyme (50, 150, 300 u/mg of protein) was monitored for 24 hours by the determinations of: hydrolysis degree dh (%), free amino group content (μmole gly/g), rp hplc peptide profiles and by polyacrylamide gel electrophoresis. in all hydrolyzates analyzed antioxidant ac ...201323520577
antibacterial and anti-inflammatory activities of mycelia of a medicinal mushroom from taiwan, taiwanofungus salmoneus (higher basidiomycetes).taiwanofungus salmoneus (t.t. chang et w.n. chou) sheng h. wu et al. (shiang-shan-chih), is a medicinal fungus indigenous to taiwan. the mycelium was prepared from submerged culture and its ethanolic and hot-water extracts were used to study its antibacterial and anti-inflammatory activities. gram-positive species (bacillus cereus, listeria monocytogenes, and staphylococcus aureus) and gram-negative species (escherichia coli and salmonella typhimurium) of bacteria were used. in addition to the i ...201323510283
selection of dna aptamers for capture and detection of salmonella typhimurium using a whole-cell selex approach in conjunction with cell sorting.alternative ligands such as nucleic acid aptamers can be used for pathogen capture and detection and offer advantages over antibodies, including reduced cost, ease of production and modification, and improved stability. dna aptamers demonstrating binding specificity to salmonella enterica serovar typhimurium were identified by whole-cell-systematic evolution of ligands by exponential enrichment (selex) beginning with a combinatorial library of biotin-labeled single stranded dna molecules. aptame ...201323494620
synthesis and antimicrobial activity of polyhalo isophthalonitrile derivatives.a series of polyhalo isophthalonitrile derivatives (3 and 4) that incorporate a variety of substituents at the 2-, 4-, 5- and/or 6-positions of the isophthalonitrile moieties have been designed and synthesized. these derivatives were evaluated for their antimicrobial activity against staphylococcus aureus, bacillus cereus (gram-positive bacteria), escherichia coli, pseudomonas aeruginosa (gram-negative bacteria); and candida albicans (fungi). compounds 3 and 4 showed stronger inhibition of gram- ...201323489634
diversity of bacillus cereus group strains is reflected in their broad range of pathogenicity and diverse ecological lifestyles.bacillus cereus comprises a highly versatile group of bacteria, which are of particular interest because of their capacity to cause disease. emetic food poisoning is caused by the toxin cereulide produced during the growth of emetic b. cereus in food, while diarrhoeal food poisoning is the result of enterotoxin production by viable vegetative b. cereus cells in the small intestine, probably in the mucus layer and/or attached to the host's intestinal epithelium. the numbers of b. cereus causing d ...201323488744
evaluation of bacterial flora during the ripening of kedong sufu, a typical chinese traditional bacteria-fermented soybean product.kedong sufu is a typical bacteria-fermented sufu in china. isolation and identification of the autochthonous bacteria involved would allow the design of specific starters for this speciality. the purpose of the present study was to evaluate the bacterial flora during the ripening of kedong sufu using polymerase chain reaction denaturing gradient gel electrophoresis (pcr-dgge) and culturing.201323400969
characterization of an atmospheric pressure plasma jet and its applications for disinfection and cancer treatment.in this work an atmospheric pressure non-thermal resistive barrier (rb) plasma jet was constructed, characterized and was applied for biomedical applications. the rb plasma source can operate in both dc (battery) as well as in standard 60/50 hz low frequency ac excitation, and it functions effectively in both direct and indirect plasma exposure configurations. the characteristics of the rb plasma jet such as electrical properties, plasma gas temperature and nitric oxides concentration were deter ...201323400199
proteolytic activity of gut bacteria isolated from the velvet bean caterpillar anticarsia gemmatalis.the development of proteinase inhibitors as potential insect control agents has been constrained by insect adaptation to these compounds. the velvet bean caterpillar (anticarsia gemmatalis) is a key soybean pest species that is well-adapted to proteinase inhibitors, particularly serine-proteinase inhibitors, which are abundant in the caterpillar host. the expression of diverse proteolytic enzymes by gut symbionts may allow the velvet bean caterpillar to circumvent proteinase inhibitors produced ...201323392900
genome sequence and analysis of a broad-host range lytic bacteriophage that infects the bacillus cereus group.comparatively little information is available on members of the myoviridae infecting low g+c content, gram-positive host bacteria of the family firmicutes. while numerous bacillus phages have been isolated up till now only very few bacillus cereus phages have been characterized in detail.201323388049
activity of the bacillus thuringiensis nprr-nprx cell-cell communication system is co-ordinated to the physiological stage through a complex transcriptional regulation.nprr is a quorum sensor of the rnpp family found in bacteria of the bacillus cereus group. in association with its cognate peptide nprx, nprr controls the expression of genes essential for survival and sporulation of bacillus thuringiensis during its necrotrophic development in insects. here, we report that the nprr-nprx genes are not autoregulated and are co-transcribed from a σ(a) -dependent promoter (pa ) located upstream from nprr. the transcription from pa starts at the onset of the station ...201323388036
structure determination through homology modelling and torsion-angle simulated annealing: application to a polysaccharide deacetylase from bacillus cereus.the structure of bc0361, a polysaccharide deacetylase from bacillus cereus, has been determined using an unconventional molecular-replacement procedure. tens of putative models of the c-terminal domain of the protein were constructed using a multitude of homology-modelling algorithms, and these were tested for the presence of signal in molecular-replacement calculations. of these, only the model calculated by the sam-t08 server gave a consistent and convincing solution, but the resulting model w ...201323385463
inhibition of bacillus cereus growth by bacteriocin producing bacillus subtilis isolated from fermented baobab seeds (maari) is substrate dependent.maari is a spontaneously alkaline fermented food condiment made from baobab tree seeds. due to the spontaneous nature of maari fermentations growth of the opportunistic human pathogen bacillus cereus is occasionally observed. bacillus subtilis strains are important for alkaline seed fermentations because of their enzymatic activities contributing to desirable texture, flavor and ph development. some b. subtilis strains have antimicrobial properties against b. cereus. in the present work, three b ...201323376785
molecular identification and safety of bacillus species involved in the fermentation of african oil beans (pentaclethra macrophylla benth) for production of ugba.molecular identification of bacillus spp. involved in the fermentation of african oil bean seeds for production of ugba, as well as ability of the bacillus spp. isolated to produce toxins, were investigated. forty-nine bacteria were isolated from ugba produced in different areas of south eastern nigeria and identified by phenotyping and sequencing of 16s rrna, gyrb and rpob genes. genotypic diversities at interspecies and intraspecies level of the isolates were screened by pcr amplification of t ...201323376783
pathogenic bacteria in finnish bulk tank milk.while the quality of raw cow milk in finland is known for its high hygienic standard, with the national average total bacterial count being below 10(4) cfu/ml annually, the prevalence of pathogenic bacteria in finnish raw milk is underreported. the aim of this study was to determine the occurrence of listeria monocytogenes, thermophilic campylobacter spp., salmonella spp., stx-positive escherichia coli (stec), coagulase-positive staphylococci, yersinia spp., and bacillus cereus group in raw cow ...201323373473
decolorization and detoxification of two textile industry effluents by the laccase/1-hydroxybenzotriazole system.the aim of this work was to determine the optimal conditions for the decolorization and the detoxification of two effluents from a textile industry-effluent a (the reactive dye bath bezactive) and effluent b (the direct dye bath tubantin)-using a laccase mediator system. response surface methodology (rsm) was applied to optimize textile effluents decolorization. a box-behnken design using rsm with the four variables ph, effluent concentration, 1-hydroxybenzotriazole (hbt) concentration, and enzy ...201323361176
antimicrobial and antifungal activities of lactobacillus curvatus strain isolated from homemade azerbaijani cheese.the aims of this study were to characterize inhibitory activity spectra, some probiotic properties and safety of lactobacillus curvatus a61 for its future application in production of fermented foods. the studied strain was isolated from traditional homemade cheese manufactured in azerbaijan. the cell-free supernatant of culture of lb. curvatus a61 inhibited the growth of tested lab, as well as of listeria monocytogenes and bacillus cereus strains. the strain presented antifungal activity and in ...201323357316
biofilm inhibitors that target amyloid proteins.bacteria establish stable communities, known as biofilms, that are resistant to antimicrobials. biofilm robustness is due to the presence of an extracellular matrix, which for several species-among them bacillus subtilis-includes amyloid-like protein fibers. in this work, we show that b. subtilis biofilms can be a simple and reliable tool for screening of molecules with antiamyloid activity. we identified two molecules, aa-861 and parthenolide, which efficiently inhibited biofilms by preventing ...201323352144
transport and retention of selected engineered nanoparticles by porous media in the presence of a biofilm.column experiments were conducted to investigate the transport of aqueous c60 (aqu-nc60), fullerol, silver nanoparticles (nps) coated with polyvinylpyrrolidone (ag-pvp) and stabilized by citrate (ag-cit) in biofilm-laden porous media. gram-negative pseudomonas aeruginosa (pa) and gram-positive bacillus cereus (bc) biofilm-laden glass beads were selected to represent the bacterial interfaces nps might encounter in the natural aquatic environment. the biomass distribution, extracellular polymeric ...201323346937
calcium-ion-induced stabilization of the protease from bacillus cereus wq9-2 in aqueous hydrophilic solvents: effect of calcium ion binding on the hydration shell and intramolecular interactions.the neutral protease wq from bacillus cereus is stable in various aqueous organic mixtures, with the exception of those containing acetonitrile (acn) and dimethylformamide (dmf). the stability of the enzyme in aqueous hydrophilic solvents was dramatically enhanced with the addition of calcium ions, with the degree of improvement in the half-life relative to different solutions ranging from fourfold to more than 70-fold. studies of the kinetic constants showed that calcium ions induced slight con ...201323322168
inhibitory effect of cinnamon powder on pathogen growth in laboratory media and oriental-style rice cakes (sulgidduk).there has been an increasing interest in the use of natural plant materials as alternative food preservatives. we examined the antimicrobial effects of natural plant materials used as additives against foodborne pathogens in laboratory media and sulgidduk, oriental-style rice cakes. cinnamon, mugwort, and garlic powder solutions (3%) were tested for their antimicrobial activities against pathogens in laboratory media. sulgidduk prepared with different amounts of cinnamon powder (1, 3, and 6%) wa ...201323317869
bacillus cereus sphingomyelinase recognizes ganglioside gm3.sphingomyelinase (smase) from bacillus cereus (bc-smase) hydrolyzes sphingomyelin (sm) to phosphocholine and ceramide in a divalent metal ion-dependent manner, and is a virulence factor for septicemia. bc-smase has three characteristic sites, viz., the central site (catalytic site), side-edge site (membrane binding site), and β-hairpin region (membrane binding site). here, we show that the β-hairpin directly binds to gangliosides, especially neuacα2-3galβ1-4glcβ1-1ceramide (gm3) through a carboh ...201323313504
bacillus cereus necrotizing pneumonia in a patient with nephrotic syndrome.bacillus cereus (b. cereus) is a gram-positive rod that is widely distributed in the environment and can be a cause of food poisoning. we herein present a case of b. cereus necrotizing pneumonia in a patient with nephrotic syndrome under corticosteroid treatment after developing transient gastroenteritis symptoms. b. cereus was isolated from bronchial lavage fluid and transbronchial biopsy specimens. a multiplex polymerase chain reaction analysis of the toxin genes revealed a strain possessing e ...201323291682
combined effects of ultrasound and surfactants to reduce bacillus cereus spores on lettuce and carrots.this study was performed to compare the effectiveness of ultrasound treatment singly and in combination with surfactants as an alternative method to conventional sanitizers containing chlorine for reducing numbers of bacillus cereus spores on fresh produce. a cocktail of three strains of b. cereus (10876, atcc 13061, and w-1) spores was inoculated onto iceberg lettuce and then treated with ultrasound for 0, 5, 10, 20 and 60 min. five minutes was found to be an adequate ultrasound (40 khz, 30 w/l ...201323290247
combined effects of plant extracts in inhibiting the growth of bacillus cereus in reconstituted infant rice cereal.a study was done to determine the potential use of plant extracts to inhibit the growth of bacillus cereus in reconstituted infant rice cereal. a total of 2116 extracts were screened for inhibitory activity against b. cereus using an agar well diffusion assay. the minimal inhibitory concentrations (mic) and minimal lethal concentrations (mlc) of 14 promising extracts in tryptic soy broth (tsb) were determined. dryopteris erythrosora (autumn fern) root extract showed the lowest mic (0.0156 mg/ml) ...201323290233
inactivation of foodborne pathogens in ground beef by cooking with highly controlled radio frequency energy.the consumer demand for fresh tasting, high quality, low salt, preservative-free meals which require minimal preparation time magnifies the safety concern and emphasizes the need to use innovative technologies for food processing. a modern technique to uniformly heat and cook foods is based on a combination of convection and controlled radio frequency (rf) energy. however any advantage conferred on meat cooked by this method would be lost if application of the technology results in decreased saf ...201323290228
assessment of the microbial safety and quality of cooked chilled foods and their production process.refrigerated processed foods of extended durability (repfeds) are a heterogeneous group of food products. this study assesses the microbial safety and quality along the production process in five repfed companies. samples were taken of raw materials (n=123), intermediate products (n=123), end products at production day (n=45) and at end of shelf life (n=90), food contact surfaces (n=226) and worker's hands/gloves (n=92). samples are analysed for total psychrotrophic aerobic count, aerobic spore ...201323290224
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