Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| does the in-utero exposure to furosemide delay the renal maturation? | two groups of pregnant wistar rats were constituted, one treated with furosemide ip and one with saline. the drug was given on days 7-11 and 14-18 of the pregnancy. litters from the two groups were not different in number of pups, body weight, and/or kidney weight, but exposed in-utero neonates, studied shortly after birth, exhibited a significant lower number of differentiated glomeruli than those of the control group. as well as the difficulty of explaining this phenomenon, this condition rais ... | 1985 | 4047752 |
| microwaves and the cell membrane. ii. temperature, plasma, and oxygen mediate microwave-induced membrane permeability in the erythrocyte. | microwaves (2450 mhz) are shown to increase 22na permeability of rabbit erythrocytes for exposures only within the narrow temperature range of 17.7 to 19.5 degrees c (tc) which coincides with a nonlinearity in the arrhenius plot reflecting an apparent membrane phase transition. significantly, this response is not observed for cholesterol-loaded erythrocyte membranes which exhibit a linear arrhenius plot and no apparent phase transition at tc. the permeability increase at tc is a nonlinear functi ... | 1985 | 4070542 |
| [the effect of large dose dexamethasone on local cerebral blood flow (l-cbf) in ischemic rat brain]. | changes of local cerebral blood flow (l-cbf) were estimated by autoradiographic techniques in middle cerebral artery occlusion rats given a large dose of (3 mg/kg) dexamethasone. the sensory-motor, parietal, auditory and olfactory cortices showed a significant reduction (greater than 25%) of l-cbf. in mca occlusion with large dose dexamethasone group, ischemic cortices showed significant reduction from the only mca occlusion group (highest 36%). there was no significant reduction of l-cbf in the ... | 1985 | 4074585 |
| structural bases of the cytolytic mechanisms of entamoeba histolytica. | the cellular bases of the powerful cytolytic activity of the human protozoan parasite entamoeba histolytica were explored by studying the effect of the virulent strain hm1:imss on epithelial monolayers of mdck cells using a combination of time-lapse microcinematography and transmission and scanning electron microscopy. early alterations of the epithelial cell membranes were detected by measuring changes in the transepithelial electrical resistance of mdck monolayers mounted in ussing chambers. t ... | 1985 | 2859368 |
| [interaction of local anesthetics with secretory cells at the membrane level]. | 1985 | 2408715 | |
| serologic diagnosis of infectious cyclic thrombocytopenia in dogs using an indirect fluorescent antibody test. | an indirect fluorescent antibody test was used for detection of serum antibodies to the platelet-specific rickettsial organism that is the causative agent of infectious cyclic thrombocytopenia (ict) in dogs. the test converted from negative to positive in 7 of 7 experimentally inoculated dogs. one of 2 attempts to recover the rickettsial agent of ict from naturally occurring seropositive dogs, by blood inoculation of experimental dogs, was successful. seemingly, the test did not detect antibodie ... | 1983 | 6362502 |
| treatment and thrombocyte levels in experimentally induced canine ehrlichiosis and canine babesiosis. | three dogs which were carriers of babesia canis were infected with ehrlichia canis. these animals developed clinical signs and haematological evidence of ehrlichiosis and made an uneventful recovery, despite the fact that they were subsequently treated with doxycycline only. three control dogs which were also carriers of b. canis were clinically normal, despite the fact that they showed a distinct drop in the thrombocyte levels associated with increases in the numbers of parasitized red cells. | 1983 | 6676688 |
| serum hyperviscosity syndrome associated with ehrlichia canis infection in a dog. | a 10-year-old male lhasa apso was examined because of pyrexia, vomiting, weight loss, lethargy, and disorientation. the clinical and laboratory findings were supportive of a diagnosis of serum hyperviscosity syndrome associated with an ehrlichia canis infection. after tetracycline therapy the serum hyperviscosity syndrome and e. canis infection were successfully resolved. | 1983 | 12002576 |
| rickettsia sennetsu in human blood monocyte cultures: similarities to the growth cycle of ehrlichia canis. | microscopic examination of cultured human monocytes infected with rickettsia sennetsu and stained by the giemsa method revealed the presence of various organismal growth forms in the cytoplasm of the infected cells. the growth forms were loosely scattered individual organisms, clusters of organisms, various sizes of dense inclusion bodies in intact and vacuolated cytoplasm, and organisms in close proximity to disintegrated monocytes. the appearance and the morphology of these r. sennetsu growth ... | 1982 | 6274798 |
| distribution of ehrlichia canis among military working dogs in the world and selected civilian dogs in the united states. | antibodies to ehrlichia canis were detected by indirect immunofluorescence in sera from 233 of 2,077 (11%) military working dogs in various locations throughout the world and from 535 of 938 (57%) civilian dogs in the united states during a 1-year period of study. overall, e canis infection rates ranged from 13% in the tropical and temperate zones below 45 degrees n to 8% in the cold zone north of 45 degrees n latitude. the highest antibody prevalence rate (24%) was found among a select populati ... | 1982 | 7050062 |
| acute experimental canine ehrlichiosis. ii. sequential reaction of the hemic and lymphoreticular system of selectively immunosuppressed dogs. | the lymphoreticular response of dogs to ehrlichia canis infection was studied after immunosuppressive therapy with cyclophosphamide or antilymphocyte serum. immunosuppression did not increase mortality, nor did it prevent or significantly modify the clinical manifestations of acute ehrlichiosis. the clinical, hematologic and serum biochemical characteristics of acute ehrlichiosis appeared seven or more days later in immunosuppressed than in immunologically intact infected dogs. immunosuppressive ... | 1981 | 7257081 |
| attempted transmission of canine ehrlichiosis to the vervet monkey (cercopithecus pygerythrus). | blood from a domestic dog infected with ehrlichia canis was injected into 3 adult vervet monkeys cercopithecus pygerythrus. the monkeys did not develop any clinical or haematological signs of disease, nor did they seem to be able to harbour the parasite. furthermore, neither morulae of e. canis nor haematological evidence of ehrlichiosis were seen after these monkeys had been splenectomized. the splenectomized monkeys did, however, develop a severe parasitaemia of their red cells with entopolypo ... | 1981 | 7312306 |
| acute experimental canine ehrlichiosis. i. sequential reaction of the hemic and lymphoreticular systems. | dogs with acute ehrlichia canis infection showed the established clinical features of acute ehrlichiosis and had thrombocytopenia, anemia, increased serum glutamic pyruvic transaminase activity, and decreased total serum protein and albumin concentrations during the first weeks after infection, and increasing gamma globulin concentrations after the third week. gross lesions in hemic and lymphoreticular tissues were transient lymphadenomegaly, splenomegaly, and increased red long-bone marrow. lym ... | 1981 | 7467072 |
| evidence of a serologic relationship between ehrlichia canis and rickettsia sennetsu. | a serologic relationship between rickettsia sennetsu, the etiologic agent of human sennetsu rickettsiosis in western japan, and ehrlichia canis, the agent of canine ehrlichiosis, has been demonstrated. using the indirect fluorescent antibody test, convalescent sera from patients with sennetsu fever reacted with ehrlichia canis antigen. in the direct fluorescent antibody test, immunoglobulins from four patients with sennetsu rickettsiosis stained e. canis morulae in canine monocytes. this finding ... | 1981 | 7034563 |
| cell-mediated and humoral immune responses of german shepherd dogs and beagles to experimental infection with ehrlichia canis. | the cell-mediated and the humoral immune responses of 12 german shepherd dogs and 5 beagles inoculated with ehrlichia canis were evaluated. results indicated that specific and nonspecific immunosuppression due to e canis occurred in the german shepherd dogs. canine leukocyte migration-inhibition factor was successfully isolated and shown to be physically and functionally similar to human and guinea pig migration inhibition factor. of the german shepherd dogs, 58% developed positive cell-mediated ... | 1980 | 6989300 |
| positive ehrlichia canis serology in kawasaki disease. | 1980 | 6103489 | |
| somatic cell hybrids of canine peritoneal macrophages and sv40-transformed human cells: derivation, characterization, and infection with ehrlichia canis. | somatic cell hybrids were obtained by fusion of canine peritoneal macrophages and sv40-transformed human skin fibroblasts. a cell line (wrh-2) was established from a single isolated hybrid clone. the wrh-2 cell line has been serially passaged 60 times and has a population doubling time of approximately 24 hours. karyotypic analysis showed the modal number of chromosomes to be 80, with a selective segregation of canine chromosomes. expression of incorporated canine dna was substantiated by cellul ... | 1980 | 6245605 |
| serial propagation of ehrlichia canis in primary canine peripheral blood monocyte cultures. | cultures of primary canine monocytes were prepared from blood to which dextran (3%) had been added to enhance sedimentation of the erythrocytes. homogeneous populations of monocytes were observed by 5 days after seeding, and some cultures were maintained as long as 30 days. the monocyte cultures were used successfully for the serial passage of ehrlichia canis. after the 3rd passage, small intracytoplasmic inclusions of e canis were detected by 60 hrs after inoculation and greater than 90% of the ... | 1980 | 7379552 |
| serum complement and blood platelet adhesiveness in acute canine ehrlichiosis. | three dogs inoculated with ehrlichia canis served as infected controls (group i), 3 dogs treated with cobra venom factor (cvf) after inoculation with e canis were the principals (group ii), 3 noninfected dogs were the noninfected controls (group iii), and 1 noninfected dog given cvf was the noninfected, decomplemented control (group iv). thrombocytopenia occurred in all e. canis-inoculated dogs (groups i and ii) within 3 weeks after they were inoculated. however, the severity of the thrombocytop ... | 1980 | 7447122 |
| free-living jackals (canis mesomelas)-potential reservoir hosts for ehrlichia canis in kenya. | using a modified cell culture test, ehrlichia canis was found in eight of 15 free-living jackals (canis mesomelas) and 14 of 31 dogs owned by pastoral communities in the same areas of kenya. two cross-bred puppies inoculated with blood from infected jackals developed mild, transient clinical disease, and e. canis was recovered from the puppies. tick species found on the jackals were similar to those found on the infected dogs. ehrlichia canis was not found in eight spotted hyaenas (crocuta crocu ... | 1980 | 7463598 |
| [intravenous cholangiography in hyperbilirubinaemia (author's transl)]. | the effect of bilirubin on biliary and renal excretion of methylglucamine-iodipamide at an infusion rate of 1.3, 2.6 and 5.2 mu mol/min/kg was studied by intravenous injection into five dogs with normal liver functions and chronic duodenal fistulas. unconjugated bilirubin administered in a dose of 0.1 mu mol/min/kg reduced biliary iodipamide excretion and concentration significantly, but did not affect renal contrast material excretion. this indicates that the unsatisficatory radiological result ... | 1979 | 153309 |
| quantitative estimation of leishmanial antibody titers by enzyme-linked immunosorbent assay. | the response to immunization against leishmania braziliensis in rabbits was followed by measurement of antibody by enzyme-linked immunosorbent assay (elisa), passive hemagglutination, complement fixation, and countercurrent immunoelectrophoresis. passive hemagglutination and complement-fixation titers were interpreted according to standard methods, whereas elisa exact titers were derived by the equation y = a-b log x, which describes the straight line that results when absorbances are plotted ag ... | 1979 | 387886 |
| [dynamics of the development of different microorganisms in soil]. | the dynamics of individual components from the complex of soil microorganisms (bacteria, fungi, actinomycetes) and the population of stm. olivocinereus introduced into soil was studied by luminescent microscopy and inoculation. the population density maxima for individual components from the complex of soil microorganisms were shown to be separated in time suggesting a succession. fungi developed at the first steps of succession and dominated in biomass over the remaining components of the compl ... | 1979 | 470632 |
| ehrlichiosis in wolves, dogs, and wolf-dog crosses. | canine ehrlichiosis was diagnosed in wolves, dogs, and wolf-dog crosses at a small zoo in northcentral florida. five of 9 adult canids and all 8 pups confined to a common kennel died as a result of the infection. the epizootic was associated with a massive rhipicephalus sanguineus infestation. hematologic and pathologic findings in an adult wolf-dog cross that died were characteristic of canine ehrlichiosis. that animal and four other canids were seropositive for ehrlichia canis. no deaths occur ... | 1979 | 521367 |
| effect of quantity and quality of igg antibodies on blocking of allergenic histamine release in vitro. | in a study of the protective role of igg antibodies in allergen injection therapy, serum antibodies to timothy grass allergens were raised in rabbits and tested for inhibition of histamine release from basophils of grass pollen-sensitive human subjects. rabbit sera were not inhibitory until late in the immune response when large amounts of relatively low affinity igg antibodies were detected by primary binding. inhibition of histamine release by late sera required preincubation of allergen and a ... | 1979 | 84798 |
| fatal adenovirus infection with misleading positive serology for infectious mononucleosis. | symptoms and serological tests in a 19-year-old man with pneumonia indicated that he had infectious mononucleosis. adenovirus type 7 was demonstrated in the lung by electron microscopy and was cultured from the liver, lung, lymph-nodes, and kidney. | 1979 | 84950 |
| safety of hepatitis-b vaccines containing intact 20 nm particles. | 1979 | 85121 | |
| expression in escherichia coli of chemically synthesized genes for human insulin. | synthetic genes for human insulin a and b chains were cloned separately in plasmid pbr322. the cloned synthetic genes were then fused to an escherichia coli beta-galactosidase gene to provide efficient transcription and translation and a stable precursor protein. the insulin peptides were cleaved from beta-galactosidase, detected by radioimmunoassay, and purified. complete purification of the a chain and partial purification of the b chain were achieved. these products were mixed, reduced, and r ... | 1979 | 85300 |
| [mechanisms of the potentiating and blocking effects of the sera from tumor patients in the leukocyte migration inhibition reaction model]. | in the model of the reaction of human blood leucocyte migration inhibition the in vitro study was conducted on some mechanisms of blocking and enhancement effects of the serum in 54 tumor patients. both effects were shown to be specific to an antigen. the enhancement effect could render a nonspecific action on intact cells in the presence of the corresponding antigen. this effect is thermolabile and may occur due to the interaction with cells. the blocking effect is thermostabile and may be real ... | 1979 | 85367 |
| the patterns and distribution of euchrysine-binding grains in nonstimulated human peripheral t and non-t lymphocytes. | 1979 | 86386 | |
| effects of antiprostatic agents on 5alpha-dihydrotestosterone binding to rat hypophyseal and hypothalamic cytosol macromolecules. | the binding of 5alpha-dihydrotestosterone to the hypophyseal and hypothalamic cytosol macromolecules prepared from castrated male rats was observed. the effects of antiprostatic agents on 5alpha-dihydrotestosterone binding to both hypophyseal and hypothalamic cytosol macromolecules was examined. cyproterone acetate and chlormadionone acetate showed the significant inhibiting effects on 5alpha-dihydrotestosterone binding to 7-8 s macromolecules of cytosol from both hypophysis and hypothalamus. sc ... | 1979 | 86441 |
| treatment of mammalian ova for the demonstration of nucleoprotein structures. | 1979 | 86485 | |
| specificity of human lymphocytes primed against allogeneic cells in vitro. ii. discrimination and cross-reactivity in repeated priming. | when studying the specificity of human lymphocytes primed in vitro against hla-d determinants on allogeneic cells, it was found that specific restimulation of the primed cells 10 days after the first priming did not influence their discriminatory power compared to cells primed only once. likewise, priming to one hla-d determinant and repeated priming against another hla-d determinant did not change the discrimination for the first priming antigen. neither was there any increased relative reactiv ... | 1979 | 87030 |
| binding studies with antibodies having phosphorylcholine specificity and fragments derived from their homologous streptococcus pneumoniae type 27 capsular polysaccharide. | the capsular polysaccharide from streptococcus pneumoniae type 27 (s-27) has been fragmented into oligosaccharides by controlled degradations. binding of these derived small ligands with heterogeneous rabbit anti-s-27 antibody of selected phosphorylcholine affinity indicates that a major immunodeterminant of s-27 is the phosphorylcholine moiety, but that these anti-pc. antibodies are also capable of binding to the polysaccharide backbone from which the p.c. has been removed. | 1979 | 87460 |
| guinea pig transfer factor: restriction of macrophage migration inhibitory activity to extraordinary dilution confirmed with demonstration of diminished potency of stored preparations. | 1979 | 88292 | |
| detection of adenovirus by enzyme-linked immunosorbent assay. | a solid-phase direct enzyme-linked immunosorbent assay (elisa) was developed for the detection of adenovirus antigen in extracts of infected cells by using antihexon serum. results with simulated clinical specimens consisting of normal nasal wash specimens seeded with varying concentrations of adenovirus type 5 showed that antigen could be detected in extracts of hep-2 cell cultures inoculated with 10(2.5) 50% tissue culture infective doses (tcid50) and 10(1.5) tcid50 after 2 and 4 days of incub ... | 1979 | 88457 |
| characterization of functional fc-receptor material from human lymphoblastoid cell lines. ii. serologic and cellular analysis. | 1979 | 88475 | |
| studies on mononuclear phagocyte progenitor cells: morphology of cells and colonies in liquid culture of mouse bone marrow. | 1979 | 88518 | |
| a technique for investigating the antigenicity of cultured rheumatoid synovial cells. | antisera to rheumatoid and nonrheumatoid synovial cells were prepared by: intraarticular injection of rabbits with these cells, subsequent synovectomy, and autologous immunization of each animal with the cultured rabbit synovial cells, or homogenized whole rabbit synovial membrane. resultant antisera were studied by immunofluorescence and 51chromium cytotoxicity tests against other cultured rheumatoid and nonrheumatoid cells. activity of positive sera could be removed by absorption with nonrheum ... | 1979 | 88519 |
| use of antibodies for studying the sidedness of membrane components. | 1979 | 88659 | |
| [mrna of mouse plasmacytoma. reverse transcription and translation in cell-free systems]. | total poly(a)-mrna from polyribosomes of mopc 21 mouse myeloma were investigated. poly(a)-mrna was released by two successive chromatography on oligo (dt)-cellulose. a 14s fraction of total poly(a)-mrna was obtained and partially purified by sucrose gradient centrifigation followed by acrylamide gel electrophoresis. as estimated from the electrophoretic analysis, the 14s mrna has three components, one of which appears to be 18s rrna and two others--mrnas with molecular weight of 5.2.10(5) and 3. ... | 1979 | 88667 |
| the influenza virus: antigenic composition and immune response. | the architecture and chemical composition of the influenza virus particle is described with particular reference to the protein constituents and their genetic control. the dominant role in infection of the surface proteins - haemagglutinins and neuraminidases - acting as antigens and undergoing variation in time known as antigenic drift and shift is explained. the immuno-diffusion technique has illuminated the interrelationships of the haemagglutinins of influenza a viruses recovered over long p ... | 1979 | 88726 |
| inhibition of spontaneous transformation of rat embryo cells releasing endogenous type c virus by virus-specific antiserum. | noninbred sprague-dawley rat embryo cell clones predictably undergo transformation after 20-30 in vitro passages following spontaneous release of endogenous rat leukemia virus (ralv). in the presence of ralv-specific antiserum, virus production and infectivity were reduced and transformation was delayed from 6 to 25 weeks. transformation was not associated with an increased expression of kirsten murine sarcoma virus-related src gene rna. | 1979 | 89211 |
| amylase levels in the tissues and body fluids of the domestic cat (felis catus). | 1. the amylase activities of serum, liver, pancreas, submaxillary and parotid glands, saliva, duodenum, colon, lung, heart, spleen, kidney and skeletal muscle of the domestic cat, were determined. 2. as in most mammals, the highest amylase content was found in the pancreas. levels in saliva and salivary glands were very low. 3. amylase activities of the serum, liver and pancreas have an optimum ph 7.0-7.5. 4. amylases of serum, liver and pancreas were inactivated by removal of chloride and these ... | 1979 | 95689 |
| a model of biliary pancreatic reflux. | a primate model for the study of biliary pancreatic reflux under relatively physiological conditions is described. cannulas were inserted into the gallbladder and the common bile duct of rhesus monkeys, and a pedicled segment of small bowel was used to create a pancreaticocutaneous fistula after resection of the spleen and pancreatic tail. following recovery, hypaque was instilled into the gallbladder with maintenance of common duct pressure within a normal range. the pancreatic duct was visuali ... | 1979 | 105415 |
| interference of iodine-125 ligands in radioimmunoassay: evidence implicating thyroxine-binding globulin. | although there is abundant published evidence that radioiodinated antigens interfere in digoxin radioimmunoassays, other radioimmunoassays are similarly affected. we investigated the relationship of radioiodinated antigen structure to its function in the immunoassay. carrier-free 125i-labeled iodotyrosine and iodohistamine derivatives were incubated with human serum, and the bound and free fractions were separated. we demonstrated that diiodotyrosyl analogs bind avidly to serum proteins. because ... | 1979 | 108034 |
| specificity and biological distribution of coenzyme m (2-mercaptoethanesulfonic acid). | the specificity of the growth requirement of methanobacterium ruminantium strain m1 for a new coenzyme, 2-mercaptoethanesulfonic acid (hs--com), was examined. a variety of derivatives, analogs, and potential biosynthetic precursors of coenzyme m were tested; only a restricted range of thioether, thioester, and thiocarbonate derivatives of the cofactor were found to replace the hs--com requirement. bromoethanesulfonic acid (brch2ch2so3-), a halogenated analog of hs--com, potently inhibited the gr ... | 1979 | 104960 |
| influence of adrenocorticotropin on transport of a cholesteryl linoleate-low density lipoprotein complex into adrenal tumor cells. | the action of adrenocorticotropin (acth) on the specific (receptor-mediated) uptake of cholesteryl linoleate . low density lipoprotein complexes was examined in y-1 mouse adrenal tumor cells. high affinity binding (ka 4.1 x 10(8) m) was observed with acth; lower affinity was seen in the absence of acth. the effect of acth was observed within 10 min at physiological concentrations of low density lipoprotein (100 microgram/ml). binding was followed by uptake (internalization) of the ester . lipopr ... | 1979 | 227899 |
| a slowly dissociating form of the cell surface cyclic adenosine 3':5'-monophosphate receptor of dictyostelium discoideum. | experiments using a phosphodiesterase-minus mutant of dictyostelium discoideum indicate that ligand-induced loss of cell surface cyclic adenosine 3':5'-monophosphate binding sites (down regulation) can be evoked with concentrations of cyclic adenosine 3':5'-monophosphate as low as 10(-8) m. the loss of receptor sites is observed after 5 min of cell preincubation with cyclic adenosine 3':5'-monophosphate and can be as extensive as 75 to 80%. this decrease in binding sites is correlated with the a ... | 1979 | 227901 |
| marek's disease lymphoma of chicken and its causative herpesvirus. | 1979 | 228727 | |
| isolation and partial biochemical characterization of the brush border plasma membrane from the cestode, hymenolepis diminuta. | 1979 | 229204 | |
| effects of althesin and its steroidal components on a variety of excised smooth muscle preparations. | 1979 | 262472 | |
| influence of the substituent radicals in antimonyl naphthalein derivatives on the potentialities of anti-schistosomal activity in mice. | 1979 | 262474 | |
| the measurement of bile acid-dependent and independent fractions of rat bile. | bile flow and bile acid secretory rate in rate in rats with interruption of the enterohepatic circulation had a biphasic decay with the second slower slope less marked for bile flow. a direct relationship between both parameters was observed in these animals. by knowing the choleretic efficiency of bile acids calculated from the regression line, the total bile acid output and the volume of bile collected, the fraction of bile produced by other way than bile water carried by bile acids was estima ... | 1978 | 263162 |
| specific labelling of replicating spp1 dna: analysis of viral dna synthesis and identification of phage dna-genes. | specific labelling of replicating bacteriophage spp1 dna can be achieved by infection at nonpermissive temperature of a b. subtilis strain carrying the initation mutation dnab ts134. under these conditions host dna synthesis is reduced by 90 to 95%. this technique was used to identify cistrons of spp1 involved in phage dna synthesis and to define intermediates in spp1 replication. | 1978 | 105242 |
| giant group a marker chromosome in three human lymphoblastoid cell strains from nasopharyngeal carcinoma. | 1978 | 95929 | |
| hematological data of the laboratory-maintained bonnet (macaca radiata) and the langur (presbytis entellus entellus) monkeys. | 1978 | 95998 | |
| ultrastructural localization of calcium around the membrane of the surface connected system in the human platelet. | the localization of calcium in the membrane system of human platelets was determined by ultrahistochemical methods equipped with an electron probe x-ray microanalyzer. after potassium oxalate-glutaraldehyde treatment large amounts of electron opaque precipitates were observed around the membrane of the surface connected system. electron probe x-ray microanalysis clearly defined that the precipitates were composed of calcium oxalate. the localization of calcium on the membrane of the surface conn ... | 1978 | 96043 |
| [adaptation of lipase and phospholipase a activities in pancreas and pancreatic juice in rats with diets rich in triglycerides and phospholipids]. | lipid rich diets containing about 20% of triglycerides or phospholipids given to rats during 2 months were observed to increase lipase and phospholipase a2 activities in pancrease and pancreatic juice. the phospholipase and lipase activities are higher, respectively, on the phospholipid and triglyceride diet. lower effects are observed after a 7-day administration of diet containing 40% of total lipid. | 1978 | 96963 |
| [the effects of testosterone and estradiol on the activity of lysosomal enzymes in rat liver and kidneys]. | in in vitro tests with lysosomes isolated from the liver and kidneys of castrated rats of both sexes the action of testosterone and beta-estradiol in concentrations of 3.76.10(-4)m on the activity of beta-glucosidase, beta-galactosidase and acid phosphatase was investigated. testosterone is shown to reduce the total and free activity of the membrane-bound enzymes and to increase the release from the matrix lysosomes. estradiol proved less active than is testosteron. the renal lysosomes in vitro ... | 1978 | 97095 |
| induction of arylhydrocarbon hydroxylase and udp glucuronosyl transferase by pcb in hepatic cell cultures. | the purpose of the investigation was to derive a hepatic cell line to be used in induction studies of drug metabolizing enzymes. two pure cell lines were isolated from primary liver cell cultures, one with an epithelial-like appearance, the other with a fibroblast-like appearance. the specific activities of arylhydrocarbon hydroxylase (ahh) and udp glucuronosyl transferase (udpgt) were greater in hepatocyte cultures than in primary cultures or in fibroblast cultures. pcb enhanced the activity of ... | 1978 | 98133 |
| [immuno-electro-diffusion on cellulose acetate membrane for serological testing in human malaria (author's transl)]. | an immuno-electro-diffusion assay for human malaria, using cellulose acetate gel, is described. more than 3500 sera have been tested by this method, which proved very useful for epidemiology since 400 sera a day can be tested by one person, with simple equipment. its sensitivity compared well to the ifa test, although the antibodies revealed are quite different. a constnat precipitin line appeared, against 56 degrees c labile antigens, in sera of subjects infected by p. falciparum, and up to 8 o ... | 1978 | 98748 |
| guinea pig immunoglobulin light chain isotypes. i. separation of kappa and lambda chains and the identification of three isotypes of the lambda chain constant homology region. | the separation of intact kappa chain and two fragments comprising lambda (lambda) chain from immunoglobulin light chain pools isolated from strain 13 guinea pigs is achieved by cyanogen bromide digestion and gel filtration before and after reductive clevage of disulfide bonds. the smaller lambda chain fragment derives from the original carboxyl-terminus of lambda chain. the partial sequence of component tryptic and thermolytic peptides of this thirty-nine residue fragment allowed its complete se ... | 1978 | 103729 |
| transfer of 2,4,5,2',4',5'-hexachlorobiphenyl and 2,2-bis-(p-chlorophenyl),1,1,1-trichloroethane (p,p'-ddt) from maternal to newborn and suckling rats. | pregnant rats were given a small dose of 14c-2,4,5,2',4',5'-hexachlorobiphenyl (hcb) and 3h-ddt intraperitoneally. the transfer of hcb and ddt through the placenta and milk was then investigated. transfer through the placenta was 2.7 and 1.5% (respectively) of the initial doses; transfer through milk was 39.2 and 21.5%. hcb is obviously more transferable than ddt through the placenta and milk, the ratio of the amount of hcb transferred through milk to the amount transferred through the placenta ... | 1978 | 104695 |
| retrospective study of an ehrlichia canis epizootic around phoenix, arizona. | antibodies to ehrlichia canis were detected by indirect immunofluorescence in sera from 40 of 339 dogs (12%) examined from around phoenix, az, during 1975. the prevalence was higher in purebred and crossbred german shepherd dogs (21%), as compared with 8% in the remaining breeds. the difference in antibody prevalence between sexes was not significant. dogs 12 weeks to 13 years old were seropositive. the causative agent was isolated by inoculation of whole blood from a seropositive dog into a sus ... | 1978 | 624663 |
| cyclic thrombocytopenia induced by a rickettsia-like agent in dogs. | hematologic manifestations and the ultrastructure of a platelet-specific microorganism isolated from a dog in florida were studied. the agent was readily transmitted experimentally to adult dogs by intravenous inoculation with infected blood. parasitemias and concomitant thrombocytopenias were cyclic in that both recurred within relatively constant periods of one to two weeks following experimental infections. hemorrhage was not a manifestation of the disease even though thrombocytopenias were s ... | 1978 | 627738 |
| effect of canine immune serum on the growth of ehrlichia canis within nonimmune canine macrophages. | the effect of canine immune serum (cis) on the growth of ehrlichia canis was studied in macrophage cultures derived from peripheral blood monocytes of normal dogs. ehrlichiae treated with canine normal serum and then introduced into normal macrophage cultures maintained in canine normal serum multiplied within the macrophages and destroyed them. immune serum, collected from e canis carrier dogs, suppressed ehrlichial growth in normal macrophages. an inverse relationship existed between the amoun ... | 1978 | 629451 |
| effect of canine immune macrophages and canine immune serum on the growth of ehrlichia canis. | the effect of canine immune macrophages and canine immune serum (cis) on the growth of ehrlichia canis was studied in vitro. treatment of e canis organisms with cis before they were inoculated into immune macrophage cultures, coupled with pre- and postinoculation maintenance of cultures in cis, suppressed macrophage infection rates to 1% or less. immune macrophages collected from e canis-carrier dogs and maintained in canine normal serum were more resistant to the intracellular growth of e canis ... | 1978 | 629452 |
| prophylactic and therapeutic use of tetracycline during an epizootic of ehrlichiosis among military dogs. | an epizootic of canine ehrlichiosis at the military working dog center, pakchong, thailand, was arrested by prophylactic and therapeutic use of tetracycline and by control of ticks. oral treatment with tetracycline (66 mg/kg of body weight daily for 14 days) effected remission of signs of disease in 60 of 62 clinically affected dogs and resulted in disappearance of serum antibody in 119 (60%) of 198 seropositive dogs treated during the 2-year study period. transmission of ehrlichia canis was int ... | 1978 | 640933 |
| [morphological changes in the digestive organs during prolonged space flight on the kosmos-782 biosatellite]. | a reduction in the content of neutral mucopolysaccharides in mucous cells of the neck, a slight decrease in the activity of succinate dehydrogenase and nad-diaphorase in parietal cells, a decrease in the dna synthesis rate, and an increase in the area of mitochondria and cristae were detected in the gastric mucosa of rats which were in a long-term space flight. in the small intestine, an increase in the activity of glucose-6-phosphate dehydrogenase and leucine aminopeptidase were found. morpholo ... | 1978 | 152100 |
| human bone marrow colony formation in diffusion chambers. normal values. | 1978 | 340240 | |
| nucleotide sequence of the ampicillin resistance gene of escherichia coli plasmid pbr322. | i have determined the nucleotide sequence of the ampicillin resistance gene of pbr322, an escherichia coli plasmid that encodes a penicillin beta-lactamase. this gene codes for a protein of 286 amino acid residues. the first 23 amino acids presumably form a signal for secretion, because they do not appear in the mature enzyme, whose partial amino acid sequence has been determined independently. | 1978 | 358200 |
| autologous lymphocyte-mediated cytotoxicity against monocytes in canine ehrlichiosis. | the 51cr-release technique for detecting cytotoxicity had been adapted to immunologic studies of canine ehrlichiosis. lymphocytes from dogs infected with ehrlichia canis, cause of canine ehrlichiosis, were shown to be cytotoxic for autologous monocytes. the effect was dependent on the concentraton of lymphocytes, being optimal at 100:1 lymphocyte:monocyte ratio. neither immune serum and complement, nor anti-canine globulin had any observable effect on cytotoxicity. the monocytotoxicity bore a te ... | 1977 | 931138 |
| canine peritoneal macrophages: cultivation and infection with ehrlichia canis. | canine peritoneal macrophages were obtained by repeated peritoneal lavage of dogs at 14 day intervals. intraperitoneal administration of sterile mineral oil increased the leukocyte yield approximately 20-fold and the macrophage recovery approximately 35-fold. cell recovery was maximum 7 to 21 days after administration of oil and declined slightly by 35 days. restimulation with a 2nd injection of oil promptly revitalized cell recovery. peritoneal macrophage cultures were well established by 6 day ... | 1977 | 931165 |
| acute canine ehrlichiosis: platelet survival and factor 3 assay. | ten dogs experimentally inoculated with ehrlichia canis were thrombocytopenic 14 days after inoculation. circulating platelet concentrations had declined rapidly by postinoculation day 10, but remained at or slightly below the 14-day concentration during the remainder of the 42-day experimental period. the percentage of circulating megathrombocytes also increased to 8.7 times preinoculation concentrations indicating the existence of accelerated thrombocytopoiesis. the release of platelet factor ... | 1977 | 931166 |
| the brown dog tick rhipicephalus sanguineus and the dog as experimental hosts of ehrlichia canis. | adult rhipicephalus sanguineus ticks efficiently transmitted ehrlichia canis to susceptible dogs for 155 days after detachment as engorged nymphs from a dog in the acute phase of ehrlichiosis. adult ticks that had similarly engorged on a dog in the chronic phase of ehrlichiosis failed to transmit e canis to susceptible dogs. infected but unfed adult ticks may be of greater importance than the chronically infected carrier dog as a natural reservoir of e canis. | 1977 | 596693 |
| structural studies and physical maps for the herpes simplex virus genome. | 1977 | 193820 | |
| [study of cytoplasmic rna in tissues of the rat, regenerating liver and in liver of rats with hepatoma pc-1]. | cytoplasmic rna of the rat regenerating liver and liver of rats with hepatoma pc-1 is separated into five fractions on the column of kieselgur with methylated albumin (mak). studies in the nucleotide composition showed that certain fractions of cytoplasm rna are ribosomal and messenger rna. the highest amount of radioactive precursor is incorporated into messenger rna of the rat regenerating liver and liver of the rats with hepatoma pc-1 which is eluated from the column by 0.2% solution of sodiu ... | 1977 | 201068 |
| the presence of disulfide-linked gp70-p15(e) complexes in akr murine leukemia virus. | 1977 | 201090 | |
| surface ultrastructural response of the human uterine lining epithelium to hormonal environment. a scanning electron microscopic study. | 1977 | 269610 | |
| therapy for infections due to anaerobic bacteria: an overview. | several classes of antimicrobial agents (e.g., penicillins, cephalosporins, tetracyclines, chloramphenicol, and clindamycin) are useful in treatment of infections due to anaerobic bacteria. however, certain anaerobic bacteria have shown a striking resistance to antimicrobial agents. in vitro susceptibility tests are useful for selection of optimal therapy. the choice of agent depends, to some extent, on the organisms responsible for the infection. bacteroides fragilis is the most commonly encoun ... | 1977 | 321708 |
| [the ovary and the immunity system]. | 1977 | 340059 | |
| inhibitory effects of mersalyl and of antibodies directed against smooth-muscle myosin on a calcium adenosine triphosphatase of the plasma membrane from mouse liver cells. | 1976 | 130830 | |
| development of ehrlichia canis, causative agent of canine ehrlichiosis, in the tick rhipicephalus sanguineus and its differentiation from a symbiotic rickettsia. | certain aspects of the development of ehrlichia canis, causative agent of canine ehrlichiosis (tropical canine pancytopenia) in rhipicephalus sanguineus ticks were studied. it was found that partial feeding of nymphs infected as larvae with e canis was a desirable, if not necessary, preliminary treatment for successful infection of dogs with ground-up ticks. it remains unclear whether feeding increased the number or altered the virulence of ehrlichiae within tick tissues. ehrlichia canis organis ... | 1976 | 944000 |
| identification of ehrlichia canis in east africa. | ehrlichia canis, the causative agent of canine ehrlichiosis or tropical canine pancytopaenia (tcp) has been identified in kenya. transmission studies to two dogs resulted in signs of ehrlichiosis including marked thrombocytopaenia, pyrexia, reduction in the packed cell volume and the presence of e canis in peripheral blood mononuclear cells. | 1976 | 997191 |
| pre- and postnatal enzyme capacity for drug metabolite production. | most lipid-soluble foreign compounds including drugs, insecticides and many environmental pollutants are metabolized in animals by cytochrome p-450 enzymes in the endoplasmic reticulum of liver. these enzymes are virtually absent in fetuses of laboratory animals, but their activities increase to adult levels within 3-8 weeks after birth. in human fetuses, the enzymes appear during the first half of pregnancy, and their activities during gestation reach about one third of those found in adults. t ... | 1975 | 1090453 |
| isolation in illinois of a foreign strain of ehrlichia canis, the causative agent of canine ehrlichiosis (tropical canine pancytopenia). | ehrlichiosis (tropical canine pancytopenia) was diagnosed in illinois in a 4 1/2-year-old mixed breed dog that had spent the previous 2 years in india. the dog had periodic epistaxis, anemia, leukopenia, hypoalbuminemia, and hypergammaglobulinemia. a titer of 1:160 for ehrlichia canis was detected by the indirect fluorescent antibody test (ifat). subinoculation of blood into a clinically normal dgo resulted in typical signs of canine ehrlichiosis, and e canis was identified in circulating mononu ... | 1975 | 1112743 |
| experimentally induced infection of dogs, cats, and nonhuman primates with ehrlichia equi, etiologic agent of equine ehrlichiosis. | dogs (german shepherd dogs and beagles), cates, rhesus macaques (macaca mulatta), and baboons (papio anubis) were inoculated with whrlichia equi, the etiologic agent of equine ehrlichiosis. within 3 to 7 days after inoculation, morulae were observed in the eosinophils of cats, neurtrophils of macaques and baboons, and in both neutrophils and eosinophils of dogs. the severe disease produced in horses by this agent was not a feature of e equi infection in dogs, cats, macaques, and baboons. however ... | 1975 | 1115421 |
| platelet kinetics in canine ehrlichiosis: evidence for increased platelet destruction as the cause of thrombocytopenia. | a significant (p < 0.025) increase in the mean platelet diameter occurred in five ehrlichia canis-infected dogs when platelet numbers decreased to 100,000/mul or less. maximal incorporation of [(75)se]selenomethionine into platelets of six uninfected dogs was 0.080 +/- 0.019% (mean +/- standard error) and occurred 5 to 6 days after dosage, whereas maximal incorporation was 0.036 +/- 0.004% within 2 to 3 days after dosage in seven chronically infected dogs that had thrombocytopenia. analysis of t ... | 1975 | 1140846 |
| transmission of ehrlichia canis to dogs by ticks (rhipicephalus sanguineus). | two strains of rhipicephalus sanguineus acquired ehrlichia canis by feeding as either larvae or nymphs on acutely infected dogs and, in subsequent instars, transmitted the agent to normal dogs. three strains of r sanguineus transmitted e canis as adults after their larval and nymphal stages fed on infected dogs. more than 400 adult female ticks were fed on infected dogs as larvae or nymphs or both, but none transmitted e canis transovarially. | 1975 | 1147359 |
| ehrlichia canis infection in a dog in virginia. | 1975 | 1150498 | |
| sex specific oestrogen binding in the middle hypothalamus of rats. | 1975 | 179802 | |
| malignant transformation-linked imbalance: decreased xanthine oxidase activity in hepatomas. | xanthine oxidase was decreased 2- to 10-fold in all examined rat hepatomas irrespective of the malignancy; growth rate and degrees of histological differentiation of the neoplasms. the affinity to substrate (km=6-8 mum) and the ph optimum (8.0) of the liver and hepatoma enzymes were the same. the reprogramming of gene expression, as manifested in the decreased activity of this key purine metabolizing enzyme, appears to be specific to neoplastic transformation. since glutamine prpp amidotransfera ... | 1975 | 179860 |
| kinetics of antibody response to ehrlichia canis assayed by the indirect fluorescent antibody method. | the kinetics of antibody production response to experimentally induced infection of dogs with ehrlichia canis was determined by ion-exchange and molecularsieve chromatography and by indirect fluorescent antibody (ifa) test. the first ifa antibody at 7 days after inoculation resided in immunoglobulin m (igm) and immunoglobulin a (iga) classes. at approximately 21 days after inoculation, the antibody was in igm, iga, and immunoglobulin g (igg) classes. thereafter, antibody concentrations continued ... | 1975 | 49162 |
| effect of morphine on intracranial self-stimulation behavior following brain amine depletion. | 1975 | 124807 | |
| ultrastructural aspects of cardiac lymphatic capillaries in experimental cardiac hypertrophy. | 1975 | 124810 | |
| flaujeac trait. deficiency of human plasma kininogen. | flaujeac trait plasma resembled hageman trait or fletcher trait, in that the intrinsic coagulation pathway, plasma fibinolytic pathway, kinin-forming system, permeability factor of dilution (pf/dil) phenomenon were abnormal. the defect in each assay was reconstituted by afactor separable from hageman factor or fletcher factor. this substance was an alpha-globulin with an approximate mol wt of 170,000. flaujeac plasma did not release a kinin upon incubation with kallikrein and was deficient in to ... | 1975 | 127805 |
| the nature of the protein moieties of cartilage proteoglycans of pig and ox. | proteoglycans extracted with 4m-guanidinium chloride from pig laryngeal cartilage and bovine nasal septum were purified by density-gradient centrifugation in cscl under 'associative' followed by 'dissociative' conditions [hascall & sajdera (1969) j. biol. chem. 244, 2384-2396]. proteoglycans were then digested exhaustively with testicular hyaluronidase, which removed about 80% of the chondroitin sulphate. the hyaluronidase was purified until no proteolytic activity was detectable under the condi ... | 1975 | 128355 |
| [morphological phase-contrast cinematographic studies of bone marrow and blood cells in vitro. 8. monocyte system in man]. | 1974 | 4589947 | |
| biological effects associated with the modification of intraphage dna with o-methylhydroxylamine. | 1974 | 4610346 |