Publications

in vivo dna cross-linking by cyclophosphamide: comparison of human chronic lymphatic leukemia cells with mouse l1210 leukemia and normal bone marrow cells.	alkaline elution was done on a variety of cells following cyclophosphamide (cy) treatment in vivo. cells used were l1210 leukemia, normal mouse bone marrow, and peripheral blood cells obtained from a patient with chronic lymphatic leukemia (cll). endpoints used were determination of single strand breaks, dna-dna interstrand and dna-protein cross-links. after treatment of mice with cy (4 mg/mouse), low levels of single strand breaks were observed in both l1210 leukemia and cdf1 normal bone marrow ...	1989	2731167
amyloid neuropathy in light chain multiple myeloma.	we describe a 73-year-old man with peripheral neuropathy and light chain lambda-type myeloma. a sural nerve biopsy showed the typical neuropathological picture of amyloid neuropathy. with the present case we first demonstrate amyloid deposits in peripheral nerves during light chain multiple myeloma and stress the importance of amyloidosis in the development of peripheral neuropathy.	1989	2743653
inhibin mrnas in ovine and bovine ovarian follicles and corpora lutea throughout the estrous cycle and gestation.	follicles and corpora lutea were dissected from ovine and bovine ovaries and the rna extracted and subjected to northern blot analyses for alpha- and beta a-inhibin mrnas, using bovine cdna and crna probes. a cdna probe detecting mrna for cholesterol side-chain cleavage cytochrome p-450 (p-450scc) was used as a positive control. in cattle, alpha- and beta a-inhibin mrnas were not detected in ovarian stroma, which could potentially have contained follicles up to 0.5 mm in diameter. inhibin-alpha ...	1989	2744224
homologous induction of growth hormone receptors in cultured rat hepatocytes.	the administration of growth hormone (gh) to animals has been reported to induce gh receptors in liver and adipose tissue. however, gh addition to cultured fibroblasts and lymphoblasts downregulated gh receptors, suggesting an indirect mechanism for gh upregulation of its receptors in vivo. we evaluated the direct role of gh by adding it to rat hepatocytes cultured in serum-free medium supplemented as previously described (barash et al. (1988) endocrinology 122, 1151-1158). after 3 days in cultu ...	1989	2744229
phylogenetic diversity of the rickettsiae.	small subunit rrna sequences have been determined for representative strains of six species of the family rickettsiaceae: rickettsia rickettsii, rickettsia prowazekii, rickettsia typhi, coxiella burnetii, ehrlichia risticii, and wolbachia persica. the relationships among these sequences and those of other eubacteria show that all members of the family rickettsiaceae belong to the so-called purple bacterial phylum. the three representatives of the genus rickettsia form a tight monophyletic cluste ...	1989	2753854
[herpesvirus and acute peripheral facial paresis].		1989	2756590
protein synthesis and degradation in non-cultured and in-vitro cultured rabbit blastocysts.	in 'pulse-chase' experiments synthesis and half-lives of leucine-labelled proteins were determined in rabbit blastocysts. embryos were either non-cultured controls or were cultured for 24 h or 48 h in ham's f-10 medium supplemented with homologous serum or uterine flushings. in control blastocysts protein synthesis increased by a factor of 10 between day 4 and day 5. half-lives of newly synthesized proteins were 32 h in day-4 and 99 h in day-5 control blastocysts. in-vitro culture of day-4 blast ...	1989	2760880
extracellular space in the developing retina assessed by electron microscopy: laminar and topographic distribution.	the extent of extracellular space (ecs) in the developing retina of the cat has been measured by electron microscopy in material fixed using techniques developed by others to preserve ecs. ecs is generally greater in foetal than in adult material. it is particularly marked in the plexiform layers of retina at the time of synaptogenesis and in the axon layer at the time of axon growth. the changes in ecs occur first in the central retina, and spread to the periphery. these observations suggest th ...	1989	2761786
primidone/phenobarbital-induced periodic alternating nystagmus.	a 37-year-old man with a history of seizures developed periodic alternating nystagmus (pan) along with other signs of primidone/phenobarbital toxicity. the pan gradually diminished in cycle length and intensity, finally resolving with gradual discontinuation of the drugs.	1989	2764437
[prospects for measuring the rheologic characteristics of human soft tissues based on the recording of their transverse rigidity].	it is shown by an example of a forearm that to determine objective characteristics of rheological properties of soft human tissues by measuring their transverse stiffness (k) one should first investigate the relation between k and the diameter of the used indentor (d). the nonlinearity of the dependence k(d) for indentor diameters from 2 to 8 mm is demonstrated and it is assumed that this nonlinearity is due to heterogeneity of tissue properties in the directions normal to the axis of force acti ...	1989	2765581
comparative sequence analysis of a genus-common rickettsial antigen gene.	the genes encoding the 17-kilodalton genus-common antigen have been cloned and sequenced from rickettsia conorii, rickettsia prowazekii, and rickettsia typhi. compared with the r. rickettsii sequence, this sequence had a high degree of homology within the coding and control regions (r. conorii, 99.8%; r. prowazekii, 88.1%; r. typhi, 88.7%). the 5' flanking regions, including the promoter and the transcription initiation sites, were extremely well conserved for all four species, suggesting that c ...	1989	2768201
human aldehyde dehydrogenase: coenzyme binding studies.	the binding of nadh and nad+ to the human liver cytoplasmic, e1, and mitochondrial, e2, isozymes at ph 7.0 and 25 degrees c was studied by the nadh fluorescence enhancement technique, the sedimentation technique, and steady-state kinetics. the binding of radiolabeled [14c]nadh and [14c]nad+ to the e1 isozyme when measured by the sedimentation technique yielded linear scatchard plots with a dissociation constant of 17.6 microm for nadh and 21.4 microm for nad+ and a stoichiometry of ca. two coenz ...	1989	2775711
adrenal vein catecholamines and neuropeptides during splanchnic nerve stimulation in cats.	splanchnic nerve stimulation in bursts at low (5 hz) and high (50 hz) frequency (30 v, 1 msec; train duration 1 sec; train rate 0.5/second) was employed in 10 cats under halothane anesthesia, during 10-minute periods, while blood samples were concurrently collected from the adrenal vein and femoral artery for the measurement of norepinephrine (ne), epinephrine (epi), dopamine (da), met-enkephalin (me), neuropeptide y (npy), peptide yy (pyy) and neurotensin (nt). in group i (n = 5), splanchnic ne ...	1989	2780418
the mrna for alpha 1-acid glycoprotein is deadenylated during stimulation by dexamethasone.		1989	2780624
the induction of protective immunity to leishmania major in the balb/c mouse by interleukin 4 treatment.	the ability of interleukin 4 (il4), administered subcutaneously around the cutaneous lesion in the form of hydrophilic gels, to affect the development of established leishmania major infections in the balb/c mouse was studied. il4 had a therapeutic effect on l. major lesion growth compared with control mice, causing not only resolution of the parasite lesions over a period of 10 weeks but also rendering animals resistant to reinfection. adoptive transfer of splenic t cells, obtained from il4-tre ...	1989	2786473
morphological studies of rat lingual filiform papillae.	in an attempt to elucidate the functions of three types of filiform papillae in the rat's tongue, corrosive resin casts of the microvessels in the filiform papillae were observed under the scanning electron microscope. the filiform papillae were also examined by light and transmission electron microscopy. the three dimensional architecture of the microvessel loops in the small conical filiform papillae was relatively simple with limited divergence and anastomosis in the papillae, whereas that in ...	1989	2812683
morphological studies of rat lingual filiform papillae.	in an attempt to elucidate the functions of three types of filiform papillae in the rat's tongue, corrosive resin casts of the microvessels in the filiform papillae were observed under the scanning electron microscope. the filiform papillae were also examined by light and transmission electron microscopy. the three dimensional architecture of the microvessel loops in the small conical filiform papillae was relatively simple with limited divergence and anastomosis in the papillae, whereas that in ...	1989	2812683
synthesis and copper-dependent antimycoplasmal activity of 1-amino-3-(2-pyridyl)isoquinoline derivatives. 2. amidines.	in our search for new compounds with antimycoplasmal activity, a series of aromatic amidines derived from 1-amino-3-(2-pyridyl)isoquinoline (1) was synthesized. in the presence of 40 microm copper the most active compounds show growth inhibition of mycoplasma gallisepticum in the nanomolar range. these compounds are 3 times as active as tylosin, an antimycoplasmal therapeutic agent that is used in veterinary practice. in the presence of copper, amidines derived from 1 are 2-3 times more active t ...	1989	2913309
improved sexual function in hemodialysis patients on recombinant erythropoietin: a possible role for prolactin.	as it was reported that correction of anemia in long-term hemodialysis patients by recombinant human erythropoietin (r-huepo) is associated with improved sexual function, we conducted the present study to further delineate the mechanism(s) by which this is brought about. serum prolactin, testosterone, and parathyroid hormone (pth) levels were followed during 4 months of r-huepo therapy. within 4 months of treatment with r-huepo, hematocrit values rose from 23.7 +/- 1.2 to 35.7 +/- 0.2% and hemog ...	1989	2914405
potassium secretion by the cortical collecting tubule: effect of c1 gradients and ouabain.	in various epithelia k and cl transport are molecularly coupled and kcl cotransport is dependent on the na-k pump. the present study examines 1) the effect of a bath-to-lumen cl gradient on k secretion during active na transport and 2) the effect of basolateral ouabain on k secretion and na absorption in the presence and the absence of a bath-to-lumen cl gradient. under symmetrical conditions there was significant k secretion (jk = -24.0 +/- 3.9 pmol.mm-1.min-1) and cl secretion (jcl = -15.7 +/- ...	1989	2916662
ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 3-kinase in rat and bovine brain tissues.	inositol 1,4,5-trisphosphate (ins p3) 3-kinase catalyzes the atp-dependent phosphorylation of ins p3 to inositol 1,3,4,5-tetrakisphosphate (ins p4). ca2+/calmodulin (cam)-sensitivity of ins p3 3-kinase was measured in the crude soluble fraction from rat brain and different anatomic regions of bovine brain. kinase activity was inhibited in the presence of egta (free ca2+ below 1 nm) as compared to ca2+ (10 microm free ca2+) or ca2+ (10 microm free ca2+) and cam (1 microm). ca2+-sensitivity was al ...	1988	2838022
antagonism of the analgesic effects of mu and kappa opioid agonists in the squirrel monkey.	the effects of several mu and kappa opioid agonists were examined alone and in combination with the opioid antagonist quadazocine in squirrel monkeys responding under a schedule of shock titration. under this procedure, shock was scheduled to increase once every 15 sec from 0.01 to 2.0 ma in 30 steps. five responses on a lever during the 15-sec shock period terminated the shock for 15 sec, after which the shock resumed at the next lower intensity. the intensity below which the monkeys maintained ...	1988	2843630
heterogeneity of glycine receptors and their messenger rnas in rat brain and spinal cord.	messenger rnas isolated from adult or newborn rat spinal cord were fractionated in a sucrose gradient. the fractions were injected into xenopus oocytes to determine their potencies for expression of glycine receptors (glyrs), which were then examined electrophysiologically. the sedimentation profiles disclosed two classes of glyr mrnas, one heavy and the other light. the adult spinal cord was rich in heavy glyr mrna, whereas the light glyr mrna was more abundant in neonatal spinal cord and in ad ...	1988	2845580
molecular biological characterization of equine herpesvirus type 1 (ehv-1) isolates from ruminant hosts.	isolates of equine herpesvirus type 1 (ehv-1) originating from affected antelope and cattle were compared with reference ehv-1 and ehv-4 isolates and were characterized. based on cross-neutralization, dna restriction profiles and blot-hybridization data these isolates could be characterized as ehv-1. one isolate (from an antelope) with a different restriction profile showed significant dna homology with ehv-1, partial homology with ehv-4, and little or no homology with ehv-2 and hsv-1 dnas. blot ...	1988	2849252
multiple subdural abscesses following colonic perforation--a rare complication of a ventriculoperitoneal shunt.	a case of colonic perforation by a ventriculoperitoneal shunt, its subsequent migration and protrusion from the anal orifice is reported. the shunt reservoir and ventricular catheter were removed percutaneously, and the disconnected peritoneal catheter was pulled out through the anus. blood culture grew klebsiella pneumoniae and streptococcus fecalis. ct scan showed multiple subdural abscesses with evidence of ventriculitis. removal of the shunt (as described), evacuation of subdural pus and sys ...	1988	3050846
characterization of mannoproteins from a virulent candida albicans strain and its derived, avirulent strain.	antigenic differences between a wild-type virulent strain of candida albicans 4918 (wt) and its spontaneous avirulent mutant (m-10) were found with crossed-immunoelectrophoresis and western blotting. mannoprotein fractions from yeast and mycelial forms were obtained by affinity chromatography on concanavalin a and analyzed with use of patient sera or sera from rabbits infected with live wt cells and boosted with whole extracts or rabbits immunized with purified wt cell wall preparations. a spike ...	1988	3055210
biochemical and chemical characterization of trifluoromethylglyoxal bis(guanylhydrazone), a close analog of the antileukemic drug mitoguazone.	in order to study the structure-activity relationships of bis(guanylhydrazone) type polyamine antimetabolites, trifluoromethylglyoxal bis(guanylhydrazone) (cf3-gbg), a close analog of the antileukemic drug methylglyoxal bis(guanylhydrazone) (mitoguazone, mgbg) was synthesized according to a novel modification of previous methods, yielding single crystals. single-crystal x-ray crystallography revealed the presence of an isomer different from the one detected in the case of mgbg and all other bis( ...	1988	3066048
estrogenic control of spermidine/spermine n1-acetyltransferase activity in rat uterus.		1988	3076330
[experimental chemo- and chemoendocrine therapy of human breast carcinomas serially transplanted into nude mice].	four human breast carcinoma strains serially transplanted into nude mice were used for the experimental chemotherapy and combination chemoendocrine therapy. whereas three of these strains (mcf-7, br-10, tm-61) possessed cytosol estrogen receptor (erc) and were dependent on estradiol for the tumor growth, the other strain (mx-1) without erc was hormone independent. for the chemotherapy, mitomycin c (mmc), adriamycin (adm), cyclophosphamide (cpm) and 5-fluorouracil (5-fu) were administered 3 times ...	1988	3129645
[infectivity and sensitivity to antibiotics of rickettsia prowazekii mutants of the low-pathogenic strain e cultured in chick embryos].	selection of mutants of a low pathogenic strain e of r. prowazekii is a trend in genetic investigation of this rickettsia species and one of the approaches to stabilizing the strain avirulent properties with a purpose of using in vaccine prophylaxis of typhus. the mutants of r. prowazekii, strain e selected by the authors earlier were characterized with respect to their infective capacity for chick embryos (ce) and antibiotic sensitivity. it was found that the infective capacity for ce of the er ...	1988	3132113
recognition of typhus group rickettsia-infected targets by human lymphokine-activated killer cells.	lymphokine-activated killer cells caused significant lysis of target cells infected with typhus group rickettsiae (tgr) but not cells infected with rickettsia tsutsugamushi. monoclonal and polyclonal antibodies against tgr or tgr-infected cells failed to bind specifically to infected targets, as determined by flow cytometry or by immunoprecipitation of radiolabeled cell surface proteins. major unaltered antigenic determinants of tgr therefore cannot be detected on the surface of infected targets ...	1988	3137174
[epidemiology and prevention of louse-borne typhus (a review of the literature)].		1988	3137725
[the sensitivity of a number of cell cultures to different strains of rickettsia prowazekii].	the sensitivity of some cell cultures to different r. prowazekii strains (strain e with low pathogenicity, virulent strain breinl, strains erifri and evir) has been studied with a view to the selection of an adequate culture for growing these strains and the study of their biological properties. experiments on titration in cells have revealed that 6- to 7-day primary and secondary irradiated quail fibroblasts and human amnion cells fl show the maximum sensitivity to all strains under study, comp ...	1988	3137741
proline incorporation into protein by rickettsia prowazekii during growth in chinese hamster ovary (cho-k1) cells.	both the requirement of rickettsia prowazekii for the amino acid proline for growth and rickettsial proline incorporation were determined in chinese hamster ovary (cho-k1) cells auxotrophic for proline. incubation of cells in dulbecco modified eagle medium supplemented with various concentrations of proline resulted in a range of host intracellular proline pools, as determined by both dansylation and equilibration of specific radioactivities. maximal rickettsial growth was observed only in host ...	1988	3141283
rickettsia prowazekii and the host cell: entry, growth and control of the parasite.		1988	3143519
[immunoenzyme analysis in a system of serologic evaluation of louse-borne typhus vaccines].	the blood sera of persons immunized with different typhus vaccines have been studied in the complement fixation test, the indirect hemagglutination test and the enzyme immunoassay. the data thus obtained indicate that the enzyme immunoassay is highly sensitive and can be universally used for the determination of antibodies to rickettsia prowazekii after primary and booster immunization with different typhus vaccines. this method detects specific antibodies both at an early period and, which is o ...	1988	3144818
[protective effect of various agents against ischemic neuronal damage in guinea pig hippocampal neurons studied in vitro].	a study was made of protective effect of several drugs (mannitol, phenytoin, vitamin e and dexamethasone) against the deprivation of both glucose and oxygen from the bathing medium on electrical activities of guinea-pig hippocampal neurons in vitro. using guinea-pig hippocampal slice, we recorded antidromic field potentials in the granular cell layer of the dentate gyrus with stimulating mossy fibers. "ischemia" of a slice was achieved by substracting both glucose and oxygen from the perfusing m ...	1988	3147404
creatinine excretion rates for evaluation of kidney function in children.	a protein load protocol for evaluation of kidney function was tested in normal children and pediatric renal patients. an overnight, timed urine collection was used for calculation of the baseline creatinine clearance and creatinine excretion rate. one hour following ingestion of a standardized protein meal (baked chicken), a 2-3 h urine collection was begun. the post-protein meal changes in creatinine clearance showed considerable variation in both the normal children and those with renal disord ...	1988	3153054
antibacterial action of powdered semiconductor on a serotype g streptococcus mutans (short communication).		1988	3165714
a new approach to the quality control of steroid receptor assays.	lyophilized cytosols prepared from calf uterus and human breast tumor tissue are commonly used to assess the reliability of routine steroid receptor assays. however, preanalytical error (sample preparation, storage, homogenization) cannot be detected in this way. participating laboratories were asked to mail us all their receptor results obtained over a four-month interval, and to include some information about the patients involved (age, menopausal status, nodal status). after verifying the hom ...	1988	3168219
in utero exposure to organic solvents and human neurodevelopment.	recent reports of growth and mental retardation in infants whose mothers abused solvent-containing substances, and of an association between central nervous system malformations and solvent exposure, have suggested that in utero exposure to organic solvents may have a profound effect on the development of the human brain. the present investigation compared the neurobehavioral development of 41 children whose mothers worked with organic solvents during pregnancy with a group of matched, unexposed ...	1988	3169389
postnatal development of the cutaneous flexor reflex: comparative study of preterm infants and newborn rat pups.	cutaneous flexor reflex thresholds have been used as a measure of somatosensory function: the authors measured threshold in preterm infants and newborn rat pups. preterm infants of less than 30 weeks postconceptional age (pca) had very low thresholds of less than 1.0 g, but by 37.5+ weeks pca thresholds were equivalent to those in normal term babies, although these are still well below adult levels. newborn rat pups also have very low flexor reflex thresholds, which do not approach adult levels ...	1988	3169392
autoimmune cd4+ t cells interfere with immune tolerance to a thymic-independent antigen.	the ability of autoimmune t cell subsets to interfere with tolerization of b cells can be studied by using thymic-independent ag. we have defined an abnormality within the cd4+ t cell compartment in young nzb and mrl-lpr/lpr mice by studying tolerance of spleen and b cells to the thymic independent ag, fluorescein-brucella abortus. tolerization of spleen cells is defective in mrl-lpr/lpr mice, but not mrl-+/+ or c3h.lpr mice, suggesting that the defect requires both the autosomal mrl background ...	1988	3286767
identification of three isoform patterns of human serum amyloid a protein.	three patterns of human apo-saa (serum amyloid a protein) isoforms have been identified by electrofocusing. in pattern 1, six major apo-saa isoforms of pi 6.0, 6.4, 7.0, 7.4, 7.5 and 8.0 were found. in pattern 2, the apo-saa isoforms of pi 7.4 and 8.0 were not detected, whereas in pattern 3 the pi-7.0 and -7.5 isoforms were lacking. six patients displayed apo-saa isoform pattern 1, 11 displayed pattern 2 and one displayed pattern 3.	1988	3355511
pasteurella multocida in wild mammals and birds in california: prevalence and virulence for turkeys.	samples collected from the oropharynx of wild mammals and birds trapped on 36 turkey farms in california were evaluated for the presence of pasteurella multocida. a total of 966 animals were collected from 18 premises that had experienced an outbreak of fowl cholera within the past 2-8 months; samples were collected from 16 of these 18 premises within 2-8 weeks of outbreak notification and while the infected flock was still present. a total of 939 animals were trapped from an additional 18 premi ...	1988	3382384
[self-protective measures in the emergency service in relation to the hiv problem].		1988	3388922
regional myocardial ajmaline concentration and antiarrhythmic activity for ischaemia- and reperfusion-induced arrhythmias in rats.	1. antiarrhythmic actions of ajmaline against ischaemia (left coronary artery occlusion for 15 min) and subsequent reperfusion-induced arrhythmias were investigated in anaesthetized rats. 2. ajmaline (2 mg kg-1, i.v.) was effective in suppressing ischaemia-induced arrhythmias whether given pre- or post-occlusion. 3. ajmaline diminished the reperfusion-induced arrhythmias completely when given pre-occlusion but had little effect when given post-occlusion. 4. reperfusion-induced increases in plasm ...	1988	3390652
c-myc expression and transformed phenotypes in hybrid clones between mouse plasmacytoma s194 cells and normal spleen cells or fibroblasts.	expression of the rearranged c-myc oncogene and transformed phenotypes was investigated in 2 different types of somatic cell hybrid clones between a balb/c mouse plasmacytoma line (s194) and normal allogeneic spleen cells or fibroblasts. in the parental s194 cells, one allele of the c-myc was rearranged and its 5'-flanking region was partially deleted by recombination with the immunoglobulin c alpha gene. due to this recombination, s194 cells expressed approximately 20-fold higher than normal sp ...	1988	3417371
genetic characterization of leishmania isolates at 37 enzyme loci.		1988	3417374
experimental ischemia induces cell mitosis in the adult rat retina.	experimental occlusion of the central retinal vessels in albino rats followed by reperfusion for 2 or 4 days resulted in a highly active proliferative response. mitotic figures were produced in retinal vascular cells, presumed müller cells, retinal pigment epithelial cells, and possibly other cell populations in the retina. a large number of mitoses were observed in 60-100% of 50 experimental eyes with 2, 4 or 6 hr of experimental ischemia and 2 or 4 days of reperfusion and in none of the 37 con ...	1988	3417399
common genetic pools between human populations.	a further inquiry on modern human origins, based on common genetic pool surveys of rigorously selected population samples and highly informative immunological polymorphisms, provides new evidence of an occidental-oriental population split as the origin of human gene pool divergences. the most likely ancestral genetic profile is discussed in the context of the debate raised by preliminary dna restriction fragment length polymorphism studies, which contradict the conclusions drawn from classical b ...	1988	3422217
comparison of adrenal medullary, carotid body and pc12 cell grafts in 6-ohda lesioned rats.	the survival and functional properties of dispersed cell implants of catecholaminergic cells obtained from the peripheral nervous system of adult rats (adrenal medulla and carotid body glomus cells) and pc12 cells from a rat pheochromocytoma cell line were examined following transplantation into the striatum of the adult rat. the host animals, all with unilateral 6-hydroxydopamine (6-ohda) nigrostriatal lesions, were divided into 5 groups: (1) pc12 cells transplanted into cyclosporin-a treated h ...	1988	2896533
atp-dependent glutamate uptake into synaptic vesicles from cerebellar mutant mice.	the atp-dependent glutamate uptake system in synaptic vesicles prepared from mouse cerebellum was characterized, and the levels of glutamate uptake were investigated in the cerebellar mutant mice, staggerer and weaver, whose main defect is the loss of cerebellar granule cells, and the nervous mutant, whose main defect is the loss of purkinje cells. the atp-dependent glutamate uptake is stimulated by low concentrations of chloride, is insensitive to aspartate, and is inhibited by agents known to ...	1988	2901460
epitopes of an influenza viral peptide recognized by antibody at single amino acid resolution.	antibodies raised against the synthetic peptide corresponding to the carboxy-terminal 24 amino acids (305-328) of the heavy chain of the hemagglutinin molecule of influenza virus a/x-31 (h3) bind this peptide at three antigenic sites. these sites were identified by assaying binding of polyclonal balb/c mouse antipeptide sera to the complete set of all possible di-, tri, tetra-, penta-, hexa-, hepta-, and octapeptides homologous with the 24-residue sequence. individual epitopes were defined and e ...	1988	2447184
perturbation of differentiated functions in vivo during persistent viral infection. iii. decreased growth hormone mrna.	lymphocytic choriomeningitis virus (lcmv) persistent infection that results from the inoculation of c3h/st newborn mice causes growth hormone (gh) deficiency and associated disease characterized by both reduced weight and serum glucose levels. molecular analysis of pituitary nucleic acids shows gh deficient mice have, on average, fivefold reduced levels of gh mrna although the histopathology of such gh producing cells is normal. northern blots indicate that the length of gh mrna is comparable in ...	1987	3811236
cardiovascular and neurological effects of laudanosine. studies in mice and rats, and in conscious and anaesthetized dogs.	the effects of laudanosine, a metabolite of atracurium, on the behaviour of conscious mice, rats and dogs, and on cardiovascular function in conscious and anaesthetized dogs have been evaluated: eeg studies were performed in anaesthetized dogs. in mice and rats, i.v. bolus doses of laudanosine 10-20 mg kg-1, caused convulsions and hind limb extensions; these effects were prevented by pretreatment with diazepam. after the continuous infusion of laudanosine to conscious dogs, plasma concentrations ...	1987	3828170
rickettsia prowazekii requires host cell serine and glycine for growth.	the growth requirement of rickettsia prowazekii for the amino acids serine and glycine was assessed in both wild-type cell lines and a mutant cell line. x-irradiated l929 cells supported the growth of r. prowazekii when the cells were incubated in eagle minimal essential medium supplemented with serum. in contrast, in this medium, x-irradiated vero cells did not support the growth of rickettsiae unless cycloheximide, serine, or glycine was added. other nonessential amino acids, additional glucos ...	1987	3098686
cloning and functional expression of the coxiella burnetii citrate synthase gene in escherichia coli.	the citrate synthase gene from the obligate intracellular rickettsial parasite coxiella burnetii was cloned and expressed in escherichia coli. transduction into e. coli with a c. burnetii gene library constructed in the cosmid vector phk17 resulted in the functional complementation of the glta mutation of e. coli mob154. a glta+ clone carrying 16.4 kilobase pairs of c. burnetii dna and designated pjcc959 was isolated and characterized. southern hybridization analysis confirmed that the pjcc959 c ...	1987	3104207
endoscopic findings of bleeding esophageal varices and experimental study.	it has been considered that the rupture of esophageal varices occurs with high incidences, and once bleeding occurs it sometimes cause fetal massive bleeding. however, from our experiences with the active application of emergency endoscopy, the incidence of bleeding from varix was unexpectedly low. from these results, we studied the rupture signs of esophageal varices in experiments on dogs and clinical cases. the actual rupture signs of varices were summarized as active bleeding, adhesion of fr ...	1987	3497471
vector-directed interleukin expression and infection.		1987	3499574
recognition of the tactaac box during mrna splicing in yeast involves base pairing to the u2-like snrna.	the u2 snrnp binds to the site of branch formation during splicing of mammalian pre-mrna in vitro. in saccharomyces cerevisiae the branch site is within the so-called tactaac box (uacuaac box), an absolutely conserved intron sequence required for splicing. based on the identification and sequence of a u2 analogue in yeast, a specific base pairing interaction between the uacuaac box and a highly conserved region of this snrna can be proposed. to test this hypothesis, we have taken advantage of tw ...	1987	3552247
sulpiride injection into the dorsal striatum increases methamphetamine-induced gnawing in rats.	sulpiride injected into the dorsal striatum enhanced both methamphetamine- and apomorphine-induced gnawing but blocked both when injected into the ventral striatum. the hyperlocomotion produced by methamphetamine was reduced by sulpiride injected into the dorsal striatum. the da, dopac and hva concentrations in the ventral striatum were increased 60 min after the injection of sulpiride into the dorsal striatum. possible reasons for the enhancement of methamphetamine-induced gnawing by sulpiride ...	1987	3556388
[buerger's disease: etiologic role of the rickettsiae?].	specific seroagglutination tests were performed in 70 patients with buerger's disease to detect possible antibodies to rickettsia, the test being repeated in 8 cases after activation by doxycycline administration over 10 days. in 21 patients a weil-felix test was also carried out. tests were assessed as positive in 44 cases (62.8%): 18 times for rickettsia q. 18, 14 times for rickettsia mooseri, 13 times for rickettsia burnetii, 5 times for rickettsia conorii and 3 times for rickettsia prowazeki ...	1987	3559416
hydrogen ion dependent reversible fibrillarization and hydration of chromatin in divalent cation-depleted isolated rat liver nuclei.	rat liver cell nuclei isolated in the presence of ca++ and mg++ ions were exposed to citrate buffer at different ph levels. their morphology was studied with the electron microscope and the water content measured. at a low ph the chromatin remained compact, but at neutral ph an intense fibrillarization of the chromatin was observed; and, seemed to be reversible, because of deoxyribonucleoproteins (dnp) filaments which dispersed at a ph of 7 were repacked to their previous site at a ph of 3. the ...	1987	3615642
[acid-base equilibrium in calves with enteritis].	studied were the indices of the acid-alkaline balance and the lactate level in a total of 19 black-and-white calves manifesting clinical forms of enteritis as well as in 10 clinically normal calves that belonged to the same breed. in most of the calves affected with enteritis the blood ph values proved essentially lower than those found in normal calves. differences were established between the normal and the diseased animals with regard to abe, hco-3, and pco2, the abe values with some of the a ...	1987	3617465
platelet aggregation induced by ether-linked phospholipids. 1. inhibitory actions of bovine serum albumin and structural analogues of platelet activating factor.	ether-linked lysophosphatidic acid was able to induce aggregation of platelets from various animals. feline platelets were the most sensitive followed in decreasing order by human, bovine and rabbit platelets. ono-6240 and cv-3988, which are antagonists of platelet activating factor, did not inhibit aggregation of feline platelets induced by ether-linked lysophosphatidic acid, indicating that the aggregation induced by the lysophosphatidic acid did not involve receptors for platelet activating f ...	1987	3647677
terbium-binding properties of calsequestrin from skeletal muscle sarcoplasmic reticulum.	calsequestrin (mr = 40,000) is a calcium-binding protein (kd = 1 mm, 50 sites/molecule) located within the terminal cisternae of the sarcoplasmic reticulum of skeletal muscle cells. the interaction of terbium, a calcium analog, with rabbit skeletal muscle calsequestrin was studied by fluorescence and circular dichroism spectroscopy. direct measurement of terbium binding using a fluorescence assay for terbium revealed that calsequestrin bound approx. 30 mol of terbium per mol of protein with an a ...	1987	3651471
isoperiodic bursting by magnocellular neuroendocrine cells in the rat hypothalamic slice.	1. recruitment of magnocellular neuroendocrine cells (m.n.c.s) to a repetitive burst pattern (phasic firing) is associated with increased vasopressin secretion from neurohypophysial terminals in the intact animal. based on invertebrate studies, bursts of action potentials can arise in two distinct ways: as an intrinsic property of the recorded cell or as an emergent property of synaptic interactions. 2. the majority of phasic m.n.c.s in the hypothalamic slice preparation display an endogenous pa ...	1987	3656153
dermofluorometric prediction of flap survival.	techniques to predict flap survival would help the reconstructive surgeon, but while various modalities exist, none is consistently accurate. the standard clinical method of fluorescein visualization by ultraviolet light requires such a high dose that it precludes rapid repetition. recently, assessment of cutaneous fluorescein with the dermofluorometer has allowed mini-dose fluorescein, quantification, and repetition. the fiberoptic dermofluorometer was tested on experimental and clinical flaps. ...	1987	3662349
characterization of ca2+ uptake and release by vesicles of skeletal-muscle sarcoplasmic reticulum.	homogenization of muscle gives a preparation of sealed vesicles derived from the sarcoplasmic reticulum. in the presence of atp these vesicles will initially accumulate ca2+ from the external medium and then spontaneously release this ca2+ in two phases, an initial slow phase and a faster second phase. by measuring atp concentrations in parallel with measurements of external ca2+ concentrations we have shown that the second phase of release occurs when the added atp has been exhausted, but that ...	1987	3663188
enzyme termini of a phosphocreatine shuttle. purification and characterization of two creatine kinase isozymes from sea urchin sperm.	two isozymes of creatine kinase have been purified from sperm of the sea urchin, strongylocentrotus purpuratus. one isozyme was purified from the sperm flagellum, and the other from the head. both require nonionic detergent for extraction from sperm. the flagellar isozyme is a monomeric species with an mr of 145,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 126,000 from sucrose density gradient and gel filtration analyses. creatine kinase from sperm heads was localized to ...	1987	3680241
fluorescence-microscopic visualization of chitin structures in egg shells of ascaris lumbricoides.		1987	3428767
effects of ouabain on cytosolic calcium in lymphocytes, platelets and adrenomedullary cells.	it has been suggested that a circulating inhibitor of na/k atpase can stimulate natriuresis and cause vasoconstriction in essential hypertension by stimulating transmembrane na/ca exchange to produce increased cytosolic concentrations of ionized calcium ([ca++]i) in renal tubular and arteriolar smooth muscle cells. if this inhibitor affected [ca++]i in all cell types, then clinical assays for its presence could be applied to easily accessible cells such as blood cells or platelets, and the inhib ...	1987	3429864
cytotoxic escherichia coli.		1987	3305326
pharmacokinetic studies in rats with ofloxacin and mf-934, a new fluorinated quinolone.		1987	3334569
[quantitative evaluation of sensitization of lymphoid cells to normal tissue and bacterial antigens].		1987	3149063
[biological properties of antibiotic-resistant mutants of the mildly pathogenic rickettsia prowazekii e strain].	the use of r. prowazekii strain e with low pathogenicity as live vaccine against exanthematous typhus is limited by its high specific reactogenicity, which is probably due to the reversion of the virulence of the strain. one of the approaches to the stabilization of the avirulent properties of strain e is obtaining its mutants with stable decreased pathogenic properties. the article presents the data on the infectious properties of r. prowazekii antibiotic-resistant strain e mutants obtained in ...	1987	3107281
cortical vasodilatation produced by vasoactive intestinal polypeptide (vip) and by physiological stimuli in the cat.	in chloralose-urethanized cats, vasoactive intestinal peptide (vip), applied by superfusion in steady-state concentration (10(-10)-10(-6) m) onto cortical vessels in situ resulted in a rapid concentration-dependent vasodilatation in vessels that were mildly constricted by prostaglandin f2 alpha (pgf2 alpha) (5 x 10(-5) m) or hypocarbia (paco2 = 26). the maximum dilatation produced by vip (10(-6) m) was about 60% over baseline in pial arteries and 40% in pial veins. blockade of local neuronal act ...	1987	3108270
comparison of the chinese schema and the international antigenic typing system for serotyping pseudomonas aeruginosa.	twelve strains of pseudomonas aeruginosa representing 12 serogroups in the serogrouping schema used in the people's republic of china were compared with serogroups in the international antigenic typing system (iats). the first eight groups originated in the people's republic of china, and group ii appears to have a new major antigen that is not found in the iats. groups i, iii, iv, v, vi, vii, and viii correspond to groups 11, 6, 9, 4, 8, 3, and 1, respectively, of the iats. groups ix, x, xi, an ...	1987	3108310
differences between saccharomyces cerevisiae and bacillus subtilis in secretion of human lysozyme.	saccharomyces cerevisiae secreted human lysozyme in the medium as an active form when the signal peptides of chicken lysozyme and a chicken lysozyme-aspergillus awamori glucoamylase hybrid were used, whereas it did not synthesize any human lysozyme protein by using the signal peptide of a. awamori glucoamylase. the secreted lysozyme was easily purified and crystallized. on the other hand, bacillus subtilis secreted an inactive human lysozyme, which seemed to have incorrect disulfide bonds, with ...	1987	3109419
nucleotide sequence of the rickettsia prowazekii citrate synthase gene.	the rickettsia prowazekii citrate synthase (glta) gene, previously cloned in escherichia coli, was localized to a 2.0-kilobase chromosomal fragment. dna sequence analysis of a portion of this fragment revealed an open reading frame of 1,308 base pairs that encodes a protein of 435 amino acids with a molecular weight of 49,171. this translation product is comparable in size to both the e. coli and pig heart citrate synthase monomers and to the protein synthesized in e. coli minicells containing t ...	1987	3112124
[immunization of travelers to (sub)tropical areas].		1987	3112592
implication of ornithine decarboxylase and polyamines in pancreatic growth of neonatal rats.	the current study examines the effects of alpha-difluoromethylornithine (dfmo), an irreversible ornithine decarboxylase inhibitor, on pancreatic growth and development of rat neonates. newborn rats were given daily subcutaneous injections of 300 or 500 mg kg-1 dfmo and killed after 7, 14, 21, and 28 days of treatment. pancreatic weights and dna, rna, protein, amylase and chymotrypsin total contents (per pancreas) and concentrations were evaluated at the end of each period. inhibition of body wei ...	1987	3114741
lysis of cells infected with typhus group rickettsiae by a human cytotoxic t cell clone.	cytolytic human t cell clones generated in response to the intracellular bacterium rickettsia typhi were characterized. growing clones were tested for their ability to proliferate specifically in response to antigens derived from typhus group rickettsiae or to lyse targets infected with r. typhi or rickettsia prowazekii. two clones were able to lyse targets infected with typhus group rickettsiae. one of these clones was more fully characterized because of its rapid growth characteristics. this c ...	1987	3121731
electron microscopic analysis of in vitro interaction of rickettsia prowazekii with guinea pig macrophages. i. macrophages from nonimmune animals.	monolayer cultures of peritoneal macrophages of intact guinea pigs were infected with rickettsia prowazekii (strain breinl) and examined by electron microscopy after 30 min, 4 and 24 hr post-infection (p.i.). three parallel processes developed in infected macrophages: reproduction of rickettsiae in macrophage cytoplasm, destruction in phagolysosomes and production of spheroplast-like forms. reproduction of rickettsiae yielded 2 cell types: those with dense and with light cytoplasm; they were loc ...	1987	2883858
electron microscopic analysis of in vitro interaction of rickettsia prowazekii with guinea pig macrophages. ii. macrophages from immune animals.	alike to macrophages from intact animals, reproduction, destruction and formation of spheroplast-like forms were observed in macrophages from immune guinea pigs 2 months post-infection (p.i.) with the virulent breinl strain of rickettsia prowazekii. unlike to the former, immune macrophages revealed phagolysosomes which were larger in size and contained more rickettsiae showing morphologic signs of destruction. spheroplast-like forms occurred more often and were more numerous than in intact anima ...	1987	2883859
rimcazole (bw 234 u) and regional monoamine concentrations in mouse brain.	rimcazole (bw 234 u), a novel agent with antipsychotic potential, was administered i.p. (50 mg/kg) to adult male icr mice 30 minutes before brain removal. regional hplc analysis of central monoamine levels showed no change in the dopac/da ratio for any tissue as compared with values obtained from control saline treated animals. however, the mhpg/ne ratio was increased in the septum, striatum, hypothalamus and thalamus and there was a reciprocal decrease in the 5hiaa/5ht ratio in the septum. the ...	1987	2884541
two distinct mechanisms for regulation of gamma-glutamyl transpeptidase in cultured rat hepatocytes by glucocorticoid-like steroids.	adult rat hepatocytes maintained in primary monolayer culture with defined medium were used to characterise two effects of glucocorticoid-like steroids in regulating gamma-glutamyltranspeptidase (ggt). low concentrations of glucocorticoids alone had little effect on ggt but synergistically enhanced induction of the enzyme by liver tumor-promoting xenobiotics such as 1,1,1-trichloro-2,2-bis-(4-chlorophenyl)-ethane (ddt) and hexachlorocyclohexane. the enhancing effect appears to be mediated by the ...	1987	2885003
use of enzyme immunoassay and nucleic acid hybridization for detecting sindbis virus in infected mosquitoes.	aedes aegypti mosquitoes were inoculated intrathoracically with prototype sindbis virus, held at 26.7 degrees c for from 0-95 h and placed at -70 degrees c. individual mosquitoes were tested for virus by plaque assay in vero cells, for viral rna by nucleic acid hybridization using a cloned cdna probe, and for viral protein by enzyme-linked immunosorbent assay. virus was detected by plaque assay as early as 8 h after infection. sindbis virus rna was detected by nucleic acid hybridization 18 h aft ...	1987	2890652
[dna diagnosis of huntington's chorea. application and genetic counseling in 4 involved families].	the discovery of linked dna markers permits presymptomatic and prenatal diagnosis of autosomal-dominant huntington's disease. however, family members born at 50% risk can find out if they have inherited the mutant gene only if family analyses are possible. many individuals at risk lack a sufficient number of living relatives for presymptomatic testing. however, prenatal exclusion testing in pregnancy can be offered to most probands. in four case reports we demonstrate the first clinical use of t ...	1987	2963376
cdna and derived amino acid sequence of ethanol-inducible rabbit liver cytochrome p-450 isozyme 3a (p-450alc).	administration of ethanol to rabbits is known to induce a unique liver microsomal cytochrome p-450, termed isozyme 3a or p-450alc, which is responsible for the increased oxidation of ethanol and other alcohols and the activation of toxic or carcinogenic compounds such as acetaminophen and n-nitrosodimethylamine. to further characterize this cytochrome p-450 we have identified cdna clones to isozyme 3a by immunoscreening, dna hybridization, and hybridization-selection. the cdna sequence determine ...	1987	3027695
[the library of rickettsia prowazekii genes].	the dna of rickettsia provazekii strain e was cleaved by psti restriction endonuclease under the conditions of partial restriction. the fragments were inserted into the psti site of pbr325 and cloned in this plasmid. e. coli strain hb101 was used as a recipient for cloning. 880 clones sensitive to ampicillin and resistant to tetracycline were selected from 5120 transformants. the cloning of rickettsial dna has been confirmed by the blot hybridization technique. analysis of individual and net pro ...	1987	3031491
effect of dehydrating agents on dna organization in herpes viruses.	with routine procedures of epon- or gma-embedding and a stain specific for dna, the nucleoid of mature herpes simplex virus-type 1 (hsv-1) assumes the well-known form of a short, compact, hollow cylinder or torus. a new, more complex organization of dna filaments in encapsidated hsv-1 was found in infected cells after aldehyde fixation, methanol dehydration, and lowicryl embedment. we have determined that it is the use of methanol as dehydrating agent that permits visualization of this internal ...	1987	3033063
[homology between the ribosomal and rna-polymerase genes of e. coli and vaccine strain e of rickettsia prowazekii].	the pib52 plasmid contains the ribosomal rp1a, j, k, l and rna-polymerase genes rpo c, b of escherichia coli. no homology has been found between the plasmid used as a molecular probe and the chromosomal dna from rickettsia provazekii in the blot hybridization experiments.	1987	3039361
site-specific hypomethylation of the c-myc oncogene in human hepatocellular carcinoma.	in order to examine the effect of alteration in methylation of the c-myc gene on hepatocarcinogenesis, the extent of methylation of the c-myc gene was examined in 24 tissues of hepatocellular carcinoma (hcc), 24 adjacent non-tumor liver tissues from the same patients and 16 control liver tissues by the use of restriction endonucleases. the following results were obtained. (1) the c-myc gene from hcc tissue tended to be hypomethylated in comparison with that in non-tumor liver tissue from the sam ...	1987	3040653
effect of cyclosporin a immunosuppression on primary lymphotropic herpesvirus infection in the guinea pig.	female guinea pigs pretreated for 2 days with cyclosporin a (csa), were then inoculated intranasally (in) or intraperitoneally (ip) with a lymphotropic herpesvirus (gphlv) and followed by 4 additional daily doses of csa. immunosuppressed animals did not show lymphocytosis and virus infectivity titers in their spleen, cervical lymph node and blood mononuclear cells were lower than those of oil-treated controls. while in-inoculated csa-treated animals expressed higher virus infectivity titer in th ...	1987	2832345
reactivity of monoclonal antibodies to rickettsia rickettsii with spotted fever and typhus group rickettsiae.	analysis of 15 spotted fever group (sfg) and 2 typhus group strains of rickettsiae with a panel of monoclonal antibodies revealed a number of shared and unique epitopes of the 120- and 155-kilodalton surface proteins. all of the sfg strains but neither of the typhus group strains reacted with antibody to the lipopolysaccharidelike antigen of rickettsia rickettsii; possibly the lipopolysaccharidelike antigen is the common antigen which defines the sfg. north carolina and montana strains of r. ric ...	1987	2432081
increase of enkephalin and decrease of substance p immunoreactivity in the dorsal and ventral striatum of the rat after midbrain 6-hydroxydopamine lesions.	unilateral 6-hydroxydopamine lesions of the mesostriatal dopaminergic system in the rat resulted in a decrease of substance p-immunoreactivity in the ventral striatum, and in a heterogeneously distributed increase of enkephalin-immunoreactivity in the dorsal and ventral striatum and the globus pallidus. the respective decrease and increase are caused by a lower or higher staining intensity of the peptidergic fibers, whereas no changes were found in the cell bodies.	1987	2440523
molecular nature of a cell membrane antigen specific for human t-cell acute lymphoblastic leukemia.	in the present work, we characterized the molecular nature of a t-cell acute lymphoblastic leukemia (t all) specific antigen, termed talla, which is defined by monoclonal antibody sn1. sn1 shows an extremely high specificity for t all. in the present study, sn1 was further shown not to react significantly with various normal solid tissues. talla was determined to be a glycoprotein with an approximate molecular weight of 150,000. however, the molecular nature of talla is peculiar in that heating ...	1987	2440562
protein and rna synthesis by isolated rickettsia prowazekii.	purified host-cell-free rickettsia prowazekii synthesized protein from leucine, lysine, or proline as radioactive precursor. most, if not all, of the rickettsial proteins were synthesized. the specific activities of the newly synthesized proteins in the cytoplasmic fraction and in the envelope fraction were not significantly different, indicating that both soluble and envelope proteins were synthesized and the latter were inserted into the envelope. the synthesis of protein was sensitive to chlo ...	1987	2442101