Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| in vitro study of tmao reduction by shewanella putrefaciens isolated from cod fillets packed in modified atmosphere. | a strain of shewanella putrefaciens was isolated from cod fillets in a modified atmosphere (60% co2, 30% o2, 10% n2). the effect of ph on the growth of shewanella putrefaciens and tmao reduction was studied in fish extract with citrate buffer systems at ph values from 5.8 to 6.8. the effect of the modified atmosphere (co2, o2) on the growth of s. putrefaciens and on the reduction of tmao to tma was studied using solid media from fish extract packed under variable mixtures of co2, o2 and n2. all ... | 1998 | 9602930 |
| concurrent infection due to shewanella putrefaciens and mycobacterium marinum acquired at the beach. | infection with marine bacteria is uncommon. a patient with systemic lupus erythematosus who developed concurrent infection with shewanella putrefaciens and mycobacterium marinum (m. marinum) is described. after bathing leg ulcers in sea water, severe cellulitis of the left leg with necrotic areas and extensive bullae developed. infection due to s. putrefaciens was confirmed and a long course of hospitalization, oral ciprofloxacin and skin grafting was required. during hospitalization subcutaneou ... | 1998 | 9611378 |
| predictive models as means to quantify the interactions of spoilage organisms. | the purpose of this paper is to quantify the interactions of some groups of spoilage organisms that can be usually found in refrigerated meat stored in air, such as: enterobacteriaceae, pseudomonas, acinetobacter, psychrobacter, shewanella, carnobacterium, lactobacillus, leuconostoc, brochothrix and kurthia spp. the growth of these organisms was studied in the range of temperature 2-11 degrees c and ph 5.2-6.4, which is characteristic of refrigerated meat. the main growth parameters (maximum spe ... | 1998 | 9631338 |
| sialic acid-binding lectin with antibacterial activity from the horse mussel: further characterization and immunolocalization. | a heterogeneous sialic acid-binding lectin with affinity for bacterial lps was isolated and partially characterized from hemolymph of the horse mussel modiolus modiolus.(1) using two-dimensional electrophoresis with immobilized ph gradients, the lectin revealed three subunits with different molecular weight and isoelectric points (pi); mr14 (pi approximately 5.1 and approximately 5.5), 17.5 (pi approximately 5.5) and 20 (pi approximately 4.9) kda. the affinity purified lectin existed in its nati ... | 1998 | 9639084 |
| design and application of two rapid screening techniques for isolation of mn(iv) reduction-deficient mutants of shewanella putrefaciens. | chemical mutagenesis procedures and two newly developed rapid plate assays were used to identify two mn(iv) reduction-deficient (mnr) mutants of shewanella putrefaciens. all eleven members of a set of previously isolated fe(iii) reduction-deficient (fer) mutants displayed mnr-positive phenotypes on the plate assays and were also capable of anaerobic growth on mn(iv) as the sole terminal electron acceptor. | 1998 | 9647855 |
| purification of a ccb-type quinol oxidase specifically induced in a deep-sea barophilic bacterium, shewanella sp. strain db-172f. | we investigated for the first time the respiratory chain system of a deep-sea barophilic bacterium, shewanella sp. strain db-172f. a membrane-bound ccb-type quinol oxidase, from cells grown at 60 mpa pressure, was purified to an electrophoretically homogeneous state. the purified enzyme complex consisted of four kinds of subunits with molecular masses of 98, 66, 18.5, and 15kda, and it contained 0.96 mol of protoheme and 1.95mol of covalently bound heme c per mol of enzyme. only protoheme in the ... | 1998 | 9672683 |
| sensorial and microbial effects of gaseous ozone on fresh scad (trachurus trachurus). | the bactericidal activity of gaseous ozone was investigated using a commercial ozone generator. five species of fish bacteria, pseudomonas putida, shewanella putrefaciens, brochothrix thermosphacta, enterobacter sp. and lactobacillus plantarum, were inoculated on agar surfaces and exposed to different ozonation times in a gas chamber. results showed ozone in relatively low concentrations (< 0.27 x 10(-3) g l-1) was an effective bactericide of vegetative cells of the five fish bacteria. the age o ... | 1998 | 9674134 |
| isolation of shewanella putrefaciens from a rheumatic heart disease patient with infective endocarditis. | 1998 | 9675697 | |
| volatile components associated with bacterial spoilage of tropical prawns. | analysis of headspace volatiles by gas chromatography/mass spectrometry from king (penaeus plebejus), banana (p. merguiensis), tiger (p. esculentus/semisulcatus) and greasy (metapenaeus bennettae) prawns stored in ice or ice slurry, which is effectively an environment of low oxygen tension, indicated the presence of amines at the early stages of storage (less than 8 days) irrespective of the nature of the storage media. esters were more prevalent in prawns stored on ice (normal oxygen conditions ... | 1998 | 9706797 |
| structure of the acidic polysaccharide chain of the lipopolysaccharide of shewanella alga 48055. | a lipopolysaccharide (lps) with an acidic polysaccharide chain was isolated from the bacterium shewanella alga strain 48055 and cleaved selectively at the glycosidic linkage of n-acetylneuraminic acid to give a tetrasaccharide. studies of the tetrasaccharide and the o-deacylated lps by 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, noesy, rotating-frame noe spectroscopy (roesy), and h-detected 1h, 13c heteronuclear multiple-quantum coherence (hmqc) experiments, revealed the following str ... | 1998 | 9720241 |
| isolation and sequence of omca, a gene encoding a decaheme outer membrane cytochrome c of shewanella putrefaciens mr-1, and detection of omca homologs in other strains of s. putrefaciens. | the sequence of the omca gene, which encodes a decaheme cytochrome c that is localized to the outer membrane (om) of shewanella putrefaciens mr-1, was determined. the 2202 bp nucleotide sequence of omca encodes for 734 amino acids with a predicted molecular protein mass of 78.6 kda. comparison with the amino-terminal sequence of the mature protein suggests the presence of a hydrophobic leader sequence which is cleaved during translocation of the protein to the om. this leader sequence has a lipo ... | 1998 | 9733973 |
| shewanella amazonensis sp. nov., a novel metal-reducing facultative anaerobe from amazonian shelf muds. | a new bacterial species belonging to the genus shewanella is described on the basis of phenotypic characterization and sequence analysis of its 16s rrna-encoding and gyrase b (gyrb) genes. this organism, isolated from shallow-water marine sediments derived from the amazon river delta, is a gram-negative, motile, polarly flagellated, facultatively anaerobic, rod-shaped eubacterium and has a g&c content of 51.7 mol%. strain sb2bt is exceptionally active in the anaerobic reduction of iron, manganes ... | 1998 | 9734053 |
| structure and function of flavocytochrome c3, the soluble fumarate reductase from shewanella ncimb400. | 1998 | 9765890 | |
| mechanisms of gene expression controlled by pressure in deep-sea microorganisms. | a pressure-regulated operon has been cloned and sequenced from deep-sea barophilic shewanella strains. to understand pressure-regulated mechanisms of gene expression, a regulatory element upstream of the pressure-regulated operon from shewanella sp. strain dss12 was studied. regions a and b were classified by sequence analysis. a unique octamer motif, aaggtaag, was found to be repeated in tandem 13 times in region b. an electrophoretic mobility shift assay demonstrated that a o54-like factor rec ... | 1998 | 9783159 |
| the role of biomineralization in microbiologically influenced corrosion. | synthetic iron oxides (goethite, alpha-feo.oh; hematite, fe2o3; and ferrihydrite, fe(oh)3) were used as model compounds to simulate the mineralogy of surface films on carbon steel. dissolution of these oxides exposed to pure cultures of the metal-reducing bacterium, shewanella putrefaciens, was followed by direct atomic absorption spectroscopy measurement of ferrous iron coupled with microscopic analyses using confocal laser scanning and environmental scanning electron microscopies. during an 8- ... | 1998 | 9807800 |
| molecular analysis of the trimethylamine n-oxide (tmao) reductase respiratory system from a shewanella species. | trimethylamine n-oxide (tmao) is an abundant compound of tissues of marine fish and invertebrates. during fish spoilage, certain marine bacteria can reduce tmao to nauseous trimethylamine (tma). one such bacterium has been isolated and identified as a new shewanella species, and called shewanella massilia. the anaerobic growth of s. massilia is greatly increased when tmao is added, indicating that tmao reduction involves a respiratory pathway. the tora enzyme responsible for tmao reduction is a ... | 1998 | 9813127 |
| crystal structure of oxidized trimethylamine n-oxide reductase from shewanella massilia at 2.5 a resolution. | the periplasmic trimethylamine n-oxide (tmao) reductase from the marine bacteria shewanella massilia is involved in a respiratory chain, having trimethylamine n-oxide as terminal electron acceptor. this molybdoenzyme belongs to the dimethyl sulfoxide (dmso) reductase family, but has a different substrate specificity than its homologous enzyme. while the dmso reductases reduce a broad spectra of organic s-oxide and n-oxide compounds, tmao reductase from shewanella massilia reduces only tmao as th ... | 1998 | 9813128 |
| taxonomic studies of deep-sea barophilic shewanella strains and description of shewanella violacea sp. nov. | several barophilic shewanella species have been isolated from deep-sea sediments at depths of 2,485-6,499 m. from the results of taxonomic studies, all of these isolates have been identified as strains of shewanella benthica except for strain dss12. strain dss12 is a member of a novel, moderately barophilic shewanella species isolated from the ryukyu trench at a depth of 5,110 m. on marine agar 2216 plates, this organism produced a violet pigment, whereas the colonies of other isolates (s. benth ... | 1998 | 9818352 |
| shewanella putrefaciens mtrb encodes an outer membrane protein required for fe(iii) and mn(iv) reduction. | iron and manganese oxides or oxyhydroxides are abundant transition metals, and in aquatic environments they serve as terminal electron acceptors for a large number of bacterial species. the molecular mechanisms of anaerobic metal reduction, however, are not understood. shewanella putrefaciens is a facultative anaerobe that uses fe(iii) and mn(iv) as terminal electron acceptors during anaerobic respiration. transposon mutagenesis was used to generate mutants of s. putrefaciens, and one such mutan ... | 1998 | 9829939 |
| moritella japonica sp. nov., a novel barophilic bacterium isolated from a japan trench sediment. | strain dsk1 is a novel moderately barophilic bacterium isolated from the japan trench at a depth of 6,356 m. phylogenetic analysis based on 16s ribosomal dna sequences showed that strain dsk1 represents a separate lineage with the shewanella barophiles branch and is closely related to moritella marina. comparisons of the temperature and pressure range for growth and some biochemical characteristics indicate that strain dsk1 differs from m. marina and shewanella barophilic species. furthermore, s ... | 1998 | 12501424 |
| carbohydrate oxidation coupled to fe(iii) reduction, a novel form of anaerobic metabolism. | an isolate, designated gc-29, that could incompletely oxidize glucose to acetate and carbon dioxide with fe(iii) serving as the electron acceptor was recovered from freshwater sediments of the potomac river, maryland. this metabolism yielded energy to support cell growth. strain gc-29 is a facultatively anaerobic, gram-negative motile rod which, in addition to glucose, also used sucrose, lactate, pyruvate, yeast extract, casamino acids or h2 as alternative electron donors for fe(iii) reduction. ... | 1998 | 16887653 |
| evolutionary relationships of cultivated psychrophilic and barophilic deep-sea bacteria. | evolutionary relationships of cultivated barophilic bacteria were determined. all psychrophilic and barophilic isolates were affiliated with one of five genera of the gamma subdivision of the class proteobacteria ((gamma)-proteobacteria): shewanella, photobacterium, colwellia, moritella, and a new group containing strain cnpt3. the data indicate that the barophilic phenotype has evolved independently in different (gamma)-proteobacteria genera. | 1997 | 16535621 |
| role of hydrophobicity in adhesion of the dissimilatory fe(iii)-reducing bacterium shewanella alga to amorphous fe(iii) oxide. | the mechanisms by which the dissimilatory fe(iii)-reducing bacterium shewanella alga adheres to amorphous fe(iii) oxide were examined through comparative analysis of s. alga bry and an adhesion-deficient strain of this species, s. alga rad20. approximately 100% of s. alga bry cells typically adhered to amorphous fe(iii) oxide, while less than 50% of s. alga rad20 cells adhered. bulk chemical analysis, isoelectric point analysis, and cell surface analysis by time-of-flight secondary-ion mass spec ... | 1997 | 16535706 |
| reduction of hexavalent uranium from organic complexes by sulfate- and iron-reducing bacteria. | the influence of organic-hexavalent-uranium [u(vi)] complexation on u(vi) reduction by a sulfate-reducing bacterium (desulfovibrio desulfuricans) and an iron-reducing bacterium (shewanella alga) was evaluated. four aliphatic ligands (acetate, malonate, oxalate, and citrate) and an aromatic ligand (tiron [4,5-dihydroxy-1,3-benzene disulfonic acid]) were used to study complexed-uranium bioavailability. the trends in uranium reduction varied with the nature and the amount of u(vi)-organic complex f ... | 1997 | 16535729 |
| the molecular biology of barophilic bacteria. | many microorganisms from the deep-sea display high-pressure-adapted--also described as barophilic or piezophilic--growth characteristics. phylogenetic studies have revealed that a large proportion of the barophilic bacteria currently in culture collections belong to a distinct subgroup of the genus shewanella, referred to as the "barophile branch." many of the basic properties of barophiles that enable their survival at extremes of pressure remain to be elucidated. however, several genes whose e ... | 1997 | 9680316 |
| cloning and sequence of cyma, a gene encoding a tetraheme cytochrome c required for reduction of iron(iii), fumarate, and nitrate by shewanella putrefaciens mr-1. | the cyma gene, which encodes a tetraheme cytochrome c, was cloned from shewanella putrefaciens mr-1. this gene complemented a mutant which had a tnphoa insertion in cyma and which was deficient in the respiratory reduction of iron(iii), nitrate, fumarate, and manganese(iv). the 561-bp nucleotide sequence of cyma encodes a protein of 187 amino acids with a predicted molecular mass of 20.8 kda. no n-terminal signal sequence was readily apparent; consistent with this, a cytochrome with a size of 21 ... | 1997 | 9023196 |
| protamine-induced permeabilization of cell envelopes of gram-positive and gram-negative bacteria. | the inhibitory effect of the cationic peptide protamine on listeria monocytogenes, escherichia coli, and shewanella putrefaciens has been studied in detail. the addition of protamine (10 to 1,000 micrograms/ml) resulted in inhibition of oxygen consumption after less than 1 min and loss of intracellular carboxyfluorescein and atp after 2 to 5 min. maximum antibacterial activity was reached at alkaline ph and in the absence of divalent cations. the efficient permeabilization of cell envelopes of b ... | 1997 | 9055431 |
| replication of plasmids with the p15a origin in shewanella putrefaciens mr-1. | the plasmid pacyc184 was introduced into shewanella putrefaciens mr-1 by electroporation. in 100% of the transformants examined, the plasmid was maintained as a free replicon outside the chromosome. this was the case whether or not the plasmid contained a 224-bp dna insert derived from an open-reading frame of mr-1 genomic dna. therefore, in contrast to a report in the literature, plasmids containing the p15a origin of replication can replicate freely in s. putrefaciens mr-1, and do not make con ... | 1997 | 9080705 |
| isolation and characterization of the gene encoding single-stranded-dna-binding protein (ssb) from four marine shewanella strains that differ in their temperature and pressure optima for growth. | the ssb gene, coding for single-stranded-dna-binding protein (ssb), was cloned from four marine shewanella strains that differed in their temperature and pressure optima and ranges of growth. all four shewanella ssb genes complemented escherichia coli ssb point and deletion mutants, with efficiencies that varied with temperature and ssb gene source. the shewanella ssbs are the largest bacterial ssbs identified to date (24.9-26.3 kda) and may be divided into conserved amino- and carboy-terminal r ... | 1997 | 9141679 |
| comparison of the gene expression of aspartate beta-d-semialdehyde dehydrogenase at elevated hydrostatic pressure in deep-sea bacteria. | aspartate beta-d-semialdehyde dehydrogenase genes (asd) were cloned and sequenced from a deep-sea-adapted strictly barophilic bacterium, shewanella sp. strain db6705, and a moderately barophilic bacterium, shewanella sp. strain dss12. the determined asd sequences of these two strains were very similar, and the identity of the deduced amino acids sequences was 96.2%. the 5'-ends of the asd mrna from both strains were localized at corresponding sites by primer extension analysis, and two transcrip ... | 1997 | 9163523 |
| differentiation of shewanella putrefaciens and shewanella alga on the basis of whole-cell protein profiles, ribotyping, phenotypic characterization, and 16s rrna gene sequence analysis. | seventy-six presumed shewanella putrefaciens isolates from fish, oil drillings, and clinical specimens, the type strain of shewanella putrefaciens (atcc 8071), the type strain of shewanella alga (iam 14159), and the type strain of shewanella hanedai (atcc 33224) were compared by several typing methods. numerical analysis of sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell protein and ribotyping patterns showed that the strains were separated into two distinct clusters with ... | 1997 | 9172338 |
| outer membrane cytochromes of shewanella putrefaciens mr-1: spectral analysis, and purification of the 83-kda c-type cytochrome. | the metal-reducing bacterium shewanella putrefaciens mr-1 is known to localize a majority of its membrane-bound cytochromes to its outer membrane when grown under anaerobic conditions. in this study, pyridine hemochrome spectra confirmed that these outer membrane cytochromes are c-type, and electrophoretic data demonstrated the presence of four distinct outer membrane cytochromes, with apparent molecular masses of 150, 83, 65, and 53 kda. fourth-order derivative analysis of 77 k spectra of the o ... | 1997 | 9218561 |
| biosynthesis of furan fatty acids (f-acids) by a marine bacterium, shewanella putrefaciens. | a mutant derived from shewanella putrefaciens 8cs7-4 treated with n-methyl-n'-nitro-n-nitrosoguanidine was found to produce 15-20 mg of a furan fatty acid (f-acid), 10,13-epoxy-11-methyloctadeca-10,12-dienoic acid (f18), per liter of growth medium (10-15% of total fatty acids). capillary gas chromatography-mass spectrometry and proton nuclear magnetic resonance analysis of the fatty acid methyl esters of the mutant revealed the presence of other f-acids, 8,11-epoxy-9-methylhexadeca-8,10-dienoic ... | 1997 | 9219910 |
| diversity and association of psychrophilic bacteria in antarctic sea ice. | the bacterial populations associated with sea ice sampled from antarctic coastal areas were investigated by use of a phenotypic approach and a phylogenetic approach based on genes encoding 16s rrna (16s rdna). the diversity of bacteria associated with sea ice was also compared with the bacterial diversity of seawater underlying sea ice. psychrophilic (optimal growth temperature, < or = 15 degrees c; no growth occurring at 20 degrees c) bacterial diversity was found to be significantly enriched i ... | 1997 | 9251193 |
| expression of the eicosapentaenoic acid synthesis gene cluster from shewanella sp. in a transgenic marine cyanobacterium, synechococcus sp. | the eicosapentaenoic acid (epa) synthesis gene cluster isolated from a marine bacterium, shewanella putrefaciens strain scrc-2738, was cloned and expressed in the marine cyanobacterium synechococcus sp. a broad-host-range cosmid vector, pjrd215 (10.2 kb, smr kmr), was used to clone a 38 kb insert, pepa, containing the epa synthesis gene cluster, creating plasmid pjrdepa (approx. 48 kb). this plasmid was transferred to the cyanobacterial host at a frequency of 2.2 x 10(-7). cyanobacterial transco ... | 1997 | 9274025 |
| polyunsaturated fatty acids in the psychrophilic bacterium shewanella gelidimarina acam 456t: molecular species analysis of major phospholipids and biosynthesis of eicosapentaenoic acid. | the production of eicosapentaenoic acid [20:5omega3; epa] from shewanella gelidimarina (acam 456t) was investigated with respect to growth temperature and growth on sole carbon sources. the percentage and quantitative yield of epa remained relatively constant at all growth temperatures within or below the optimal growth temperature region. at higher growth temperatures, these values decreased greatly. growth on differing sole carbon sources also influenced the percentage and amount of epa produc ... | 1997 | 9295160 |
| preparation of a naturally occurring d-erythro-(2s, 3r)-sphingosylphosphocholine using shewanella alga ns-589. | sphingosylphosphocholine, an n-deacylated derivative of sphingomyelin, has been found to be involved in many cellular events. this paper describes a new method for preparation of a d-erythro-sphingosylphosphocholine, which is naturally occurring but difficult to prepare by chemical methods, using marine bacteria as a biocatalyst. when cultured with shewanella alga ns-589 in synthetic medium, sphingomyelin was found to be efficiently converted by sphingomyelin deacylase to sphingosylphosphocholin ... | 1997 | 9323602 |
| cloning and sequence analysis of a novel hemolysin gene (vlly) from vibrio vulnificus. | a gene (vlly) encoding a novel hemolysin of vibrio vulnificus ckm-1 has been cloned and sequenced. when the vlly gene was expressed in minicells, a unique peptide of approximately 40 kda was identified. subcellular fractionation of escherichia coli cells carrying the vlly gene indicated that the vlly protein was distributed in both the cytoplasmic and the periplasmic fractions, with the notable ability to appear in the latter compartment. nucleotide sequence analysis predicted a single open read ... | 1997 | 9327548 |
| magnification of tributyl tin toxicity to oyster larvae by bioconcentration in biofilms of shewanella colwelliana. | the toxic effects of dissolved versus bioconcentrated tributyl tin (tbt) on oyster larvae were compared. water column tbt levels, which had no effect in solution, inhibited natural attachment and metamorphosis of oyster larvae on bottom surfaces due to bioconcentration by biofilms. this mechanism should be considered when evaluating heavy metal toxicity in the environment. | 1997 | 9327578 |
| skin and soft-tissue manifestations of shewanella putrefaciens infection. | shewanella putrefaciens, a saprophytic gram-negative rod, is infrequently recovered from clinical specimens. although a number of clinical syndromes have been attributed to s. putrefaciens, the pathogenic role of this agent remains largely undefined. we report 16 cases of s. putrefaciens infection that occurred at the veterans general hospital-kaohsiung in taiwan between 1990 and 1995. s. putrefaciens infection was associated with a wide clinical spectrum including bacteremia/septicemia, skin an ... | 1997 | 9332516 |
| shewanella woodyi sp. nov., an exclusively respiratory luminous bacterium isolated from the alboran sea. | thirty-four strains of nonfermentative, respiratory, luminous bacteria were isolated from samples of squid ink and seawater from depths of 200 to 300 m in the alboran sea. although these strains had a few properties similar to properties of shewanella (alteromonas) hanedai, they did not cluster phenotypically with any previously described bacterium. the nucleotide sequence of a 740-bp segment of luxa was not homologous with other known luxa sequences but clustered with the luxa sequences of shew ... | 1997 | 9336902 |
| shewanella gelidimarina sp. nov. and shewanella frigidimarina sp. nov., novel antarctic species with the ability to produce eicosapentaenoic acid (20:5 omega 3) and grow anaerobically by dissimilatory fe(iii) reduction. | a polyphasic taxonomic study was performed to characterize dissimilatory iron-reducing strains mostly isolated from antarctic sea ice. the strains were isolated from samples of congelated (land-fast) sea ice, grease ice, and ice algal biomass collected from the coastal areas of the vestfold hills in eastern antarctica (68 degrees s 78 degrees e). the strains were facultatively anaerobic, motile, and rod shaped, were capable of anaerobic growth either by fermentation of carbohydrates or by anaero ... | 1997 | 9336903 |
| isolation and characterization of a transposon mutant of shewanella putrefaciens mr-1 deficient in fumarate reductase. | a transposon mutant, designated cmtn-3, of shewanella putrefaciens mr-1 that was deficient in fumarate reduction was isolated and characterized. in contrast to the wild-type, cmtn-3 could not grow anaerobically with fumarate as the electron acceptor, and it lacked benzyl viologen-linked fumarate reductase activity. consistent with this, cmtn-3 lacked a 65 kda c-type cytochrome, which is the same size as the fumarate reductase enzyme. cmtn-3 retained the wild-type ability to use nitrate, iron(iii ... | 1997 | 9351256 |
| structure of a phosphorylated polysaccharide from shewanella putrefaciens strain s29. | a phosphorylated polysaccharide was isolated from the aqueous layer of the phenol-water extract of a non-halophilic bacterium shewanella putrefaciens strain s29. the glycosyl phosphate linkage in the polysaccharide was split under mild acid conditions to give, after borohydride reduction, a phosphorylated oligosaccharide-alditol. on the basis of sugar analysis and 1h, 13c and 31p nmr spectroscopy, including 2d cosy, relayed cosy, rotating-frame noe spectroscopy (roesy), heteronuclear 13c,1h cosy ... | 1997 | 9373938 |
| fatty acid synthesis of an eicosapentaenoic acid-producing bacterium: de novo synthesis, chain elongation, and desaturation systems. | the fatty acid synthesis systems of a shewanella sp., strain scrc-2738, that produces a large amount of eicosapentaenoic acid were investigated. two kinds of fatty acid synthesis system, de novo synthesis and chain elongation ones, were detected in the cytosol. the de novo synthesis system required an acyl carrier protein, and produced palmitoyl- and palmitoleoyl-acyl carrier proteins as final products. the chain elongation system also required an acyl carrier protein, and produced an acyl-acyl ... | 1997 | 9378728 |
| shewanella putrefaciens abscess of the lower extremity. | 1997 | 9399562 | |
| [bacteremia caused by shewanella putrefaciens]. | 1997 | 9410056 | |
| physiology and enzymology involved in denitrification by shewanella putrefaciens. | nitrate reduction to n2o was investigated in batch cultures of shewanella putrefaciens mr-1, mr-4, and mr-7. all three strains reduced nitrate to nitrite to n2o, and this reduction was coupled to growth, whereas ammonium accumulation was very low (0 to 1 micromol liter-1). all s. putrefaciens isolates were also capable of reducing nitrate aerobically; under anaerobic conditions, nitrite levels were three- to sixfold higher than those found under oxic conditions. nitrate reductase activities (31 ... | 1997 | 11536813 |
| ear infections with shewanella alga: a bacteriologic, clinical and epidemiologic study of 67 cases. | objective: to present bacteriologic and clinical data on 67 patients from the island of funen, denmark, with shewanella alga, a bacterium rarely seen in scandinavia, isolated from ear swabs. included in the study is an examination of the occurrence of s. alga in sea water around the island. methods: bacteriologic examination and antibiotic susceptibility testing of 67 clinical isolates, 11 sea-water isolates and two reference strains were conducted. clinical information was obtained from the ref ... | 1997 | 11864129 |
| polyamine distribution patterns within the families aeromonadaceae, vibrionaceae, pasteurellaceae, and halomonadaceae, and related genera of the gamma subclass of the proteobacteria. | polyamines of the four families and the five related genera within the gamma subclass of the class proteobacteria were analyzed by hplc with the objective of developing a chemotaxonomic system. the production of putrescine, diaminopropane, cadaverine, and agmatine are not exactly correlated to the phylogenetic genospecies within 36 strains of the genus aeromonas (the family aeromonadaceae) lacking in triamines. the occurrence of norspermidine was limited but not ubiquitous within the family vibr ... | 1997 | 12501353 |
| kinetics of u(vi) reduction by a dissimilatory fe(iii)-reducing bacterium under non-growth conditions. | dissimilatory metal-reducing microorganisms may be useful in processes designed for selective removal of uranium from aqueous streams. these bacteria can use u(vi) as an electron acceptor and thereby reduce soluble u(vi) to insoluble u(iv). while significant research has been devoted to demonstrating and describing the mechanism of dissimilatory metal reduction, the reaction kinetics necessary to apply this for remediation processes have not been adequately defined. in this study, pure culture s ... | 1997 | 18636514 |
| structure and properties of novel inclusions in shewanella putrefaciens. | cytoplasmic inclusions surrounded by a bilayer membrane were seen in thin sections. negatively stained and freeze-fractured preparations of shewanella putrefaciens. cells harvested from the late exponential and early stationary phase showed a higher number of these vesicles than bacteria isolated from early exponential or late stationary phase. chemical dyes for polyphosphate or poly-beta-hydroxybutyrate did not stain the material enclosed within these vesicles. elemental analysis of the materia ... | 1996 | 11536730 |
| growth of the facultative anaerobe shewanella putrefaciens by elemental sulfur reduction. | the growth of bacteria by dissimilatory elemental sulfur reduction is generally associated with obligate anaerobes and thermophiles in particular. here we describe the sulfur-dependent growth of the facultatively anaerobic mesophile shewanella putrefaciens. six of nine representative s. putrefaciens isolates from a variety of environments proved able to grow by sulfur reduction, and strain mr-1 was chosen for further study. growth was monitored in a minimal medium (usually with 0.05% casamino ac ... | 1996 | 11536738 |
| effect of modified atmosphere packaging on the tvb/tma-producing microflora of cod fillets. | cod fillets (gadus morhua) were packed under modified atmospheres, with four different gas compositions (60% co2-10% o2-30% n2, 60% co2-20% o2-20% n2, 60% co2-30% o2-10% n2, 60% co2-40% o2), and stored at 6 degrees c. plate counts were carried out after 3, 4, 5, 6 and 7 days, to follow the growth of aerobic and anaerobic bacteria, lactic acid bacteria, h2s-producing bacteria and enterobacteriaceae. the production of total volatile bases (tvb) and trimethylamine (tma), and the changes in ph of th ... | 1996 | 8880310 |
| upstream sequence of the parahydroxyphenyl-pyruvate dioxygenase (mela) coding region leading to enhanced expression in shewanella colwelliana. | hyperexpression of the enzyme, parahydroxyphenylpyruvate dioxygenase (phpph; mela), in the tyrosine degradative pathway yields excess homogentisic acid which oxidatively polymerizes to pyomelanin. depression of mela in shewanella colwelliana strain d was found to result from a single base pair transition upstream of the promoter. this was the sole lesion detected in phpph hyperexpressing strains. it is suggested that a t to c transition alters the mrna structure, exposing the ribosome binding si ... | 1996 | 8885416 |
| purification and properties of a low-redox-potential tetraheme cytochrome c3 from shewanella putrefaciens. | shewanella putrefaciens is a facultatively anaerobic bacterium in the gamma group of the proteobacteria, capable of utilizing a wide variety of anaerobic electron acceptors. an examination of its cytochrome content revealed the presence of a tetraheme, low-redox-potential (e'o = -233 mv), cytochrome c-type cytochrome with a molecular mass of 12,120 da and a pi of 5.8. the electron spin resonance data indicate a bis-histidine coordination of heme groups. reduction of ferric citrate was accompanie ... | 1996 | 8892848 |
| bacterial spoilage of meat and cured meat products. | the influence of environmental factors (product composition and storage conditions) on the selection, growth rate and metabolic activity of the bacterial flora is presented for meat (pork and beef) and cooked, cured meat products. the predominant bacteria associated with spoilage of refrigerated beef and pork, are brochothrix thermosphacta, carnobacterium spp., enterobacteriaceae, lactobacillus spp., leuconostoc spp., pseudomonas spp. and shewanella putrefaciens. the main defects in meat are off ... | 1996 | 8913812 |
| microbiological spoilage of fish and fish products. | spoilage of fresh and lightly preserved fish products is caused by microbial action. this paper reviews the current knowledge in terms of the microbiology of fish and fish products with particular emphasis on identification of specific spoilage bacteria and the qualitative and quantitative biochemical indicators of spoilage. shewanella putrefaciens and pseudomonas spp. are the specific spoilage bacteria of iced fresh fish regardless of the origin of the fish. modified atmosphere stored marine fi ... | 1996 | 8913813 |
| morphological and metabolic responses to starvation by the dissimilatory metal-reducing bacterium shewanella alga bry. | the response of the dissimilatory metal-reducing bacterium shewanella alga bry to carbon and nitrogen starvation was examined. starvation resulted in a gradual decrease in the mean cell volume from 0.48 to 0.2 micron 3 and a dramatic decrease in fe(iii) reductase activity. growth of starved cultures was initiated with o2, ferric oxyhydroxide, co(iii)-edta, or fe(iii)-bearing subsurface materials as the sole electron acceptor. microbially reduced subsurface materials reduced cro(4)2-. starvation ... | 1996 | 8953739 |
| changes in cell morphology of listeria monocytogenes and shewanella putrefaciens resulting from the action of protamine. | protamine, which is an antibacterial basic peptide, was shown to alter the cell morphology of listeria monocytogenes and shewanella putrefaciens. atomic force microscopy revealed that protamine smoothed the surface of cells, formed holes in the cell envelope, and caused fusion of s. putrefaciens cells. immunoelectron microscopy of protamine-treated cells of both l. monocytogenes and s. putrefaciens showed great damage to the cell wall and condensation of the cytoplasm. respiration of the cells w ... | 1996 | 8975598 |
| characterization of bacterial communities from activated sludge: culture-dependent numerical identification versus in situ identification using group- and genus-specific rrna-targeted oligonucleotide probes | the structures of bacterial communities were studied in activated sludge samples obtained from the aerobic and anaerobic zones of a wastewater treatment plant showing enhanced phosphorous removal. samples were analyzed by in situ hybridization with oligonucleotide probes complementary to selected regions of the 16s and 23s ribosomal rna (rrna) characteristic for defined phylogenetic entities (genera and larger groups). the microbial community structures revealed by molecular techniques were comp ... | 1996 | 8688004 |
| interaction between fish spoilage bacteria pseudomonas sp. and shewanella putrefaciens in fish extracts and on fish tissue. | the interaction between fish spoilage bacteria, pseudomonas sp. and shewanella putrefaciens, was investigated using fish extract and fish tissue as model systems. isolates of pseudomonas that produced iron chelators, siderophores, inhibited growth of s. putrefaciens in a fish-extract-agar diffusion assay but no, or only weak, antagonistic activity was seen when the medium was supplemented with iron. sterile-filtered supernatant fluid from a siderophore-producing pseudomonas grown in fish extract ... | 1996 | 8698659 |
| production of eicosapentaenoic acid from marine bacteria. | a marine bacterium, judged as a new species close to shewanella putrefaciens, was isolated from the intestinal contents of the pacific mackerel. the isolated strain scrc-2378 produced eicosapentaenoic acid (epa) as the sole polyunsaturated fatty acid, which amounted to 24-40% of the total fatty acid in the cell, which corresponded to 2% of dry cell weight. under the optimal growth conditions (ph 7.0, 20 degrees c, and grown aerobically for 12-18 h), the yield of scrc-2738 reached 15 g of dry cel ... | 1996 | 8729138 |
| study of a melanic pigment of proteus mirabilis. | the present study sought to determine whether the pigment produced by proteus mirabilis from the l-forms of various aromatic amino acids under aerobic conditions is melanic in nature. it is a black-brown pigment which behaves like a melanin in many respects, namely solubility features, bleaching by oxidizing agents and positive response to the fontana-masson assay. in the present study, for the first time, it was shown by electron spin resonance analysis that a bacterial melanin is able to act a ... | 1996 | 8761735 |
| shewanella alga bacteremia in two patients with lower leg ulcers. | the first danish cases of shewanella alga bacteremia in two patients with chronic lower leg ulcers are reported. both patients were admitted to the hospital during the same month of a very warm summer and had been exposed to the same marine environment, thereby suggesting the same source of infection. both patients survived; however, one of them had extensive myonecrosis, while the other patient had an uncomplicated course. the strains were initially believed to be shewanella putrefaciens on the ... | 1996 | 8783706 |
| synergistic iron reduction and citrate dissimilation by shewanella alga and aeromonas veronii | two bacterial isolates from great bay estuary, new hampshire, in co-culture carried out anaerobic dissimilation of citric acid with fe(iii) as the terminal electron acceptor. neither isolate oxidized citrate with fe(iii) anaerobically in axenic culture. the fe(iii) reducer, shewanella alga strain bry, did not grow anaerobically with citrate as an energy source. the citrate utilizer, aeromonas veronii, did not reduce iron axenically with a variety of electron donors including citrate. the onset o ... | 1996 | 8824150 |
| a novel phosphorimager-based technique for monitoring the microbial reduction of technetium. | a novel phosphorimager-based technique which can be used to quantify low concentrations of radionuclides is described. the technique offers several benefits, combining very high sensitivity with containment of the radioisotope in the solid state, thus minimizing disposal procedures. in this study, it was used in conjunction with paper chromatography to quantify different oxidation states of (sup99)tc in solution. the technique was used to evaluate the potential of anaerobic cultures of shewanell ... | 1996 | 16535242 |
| genotyping of heterotrophic bacteria from the central baltic sea by use of low-molecular-weight rna profiles. | the gotland deep, an anoxic basin, was investigated for its heterotrophic microflora as a station representative of the central baltic sea and as an example of a brackish water environment. one hundred twenty-three bacterial strains were isolated along the water column by use of four different cultivation procedures. high-resolution electrophoresis of the low-molecular-weight (lmw) rna (5s rrna and trna) was used for analysis of the taxonomic position of the strains. the banding pattern of the l ... | 1996 | 16535296 |
| deflocculation of activated sludge by the dissimilatory fe(iii)-reducing bacterium shewanella alga bry. | the influence of microbial fe(iii) reduction on the deflocculation of autoclaved activated sludge was investigated. fe(iii) flocculated activated sludge better than fe(ii). decreasing concentrations of fe(iii) caused an increase in sludge bulk water turbidity, while bulk water turbidity remained relatively constant over a range of fe(ii) concentrations. cells of the dissimilatory metal-reducing bacterium shewanella alga bry coupled the oxidation of h(inf2) to the reduction of fe(iii) bound in sl ... | 1996 | 16535299 |
| a pressurized chemostat for the study of marine barophilic and oligotrophic bacteria. | a continuous culture system that allows bacteria to be grown in steady-state populations under pressures of up to 700 atm (71 mpa) was constructed and tested. with readily available or slightly modified high-pressure chromatography equipment, a continuous flow of sterile medium is pressurized and passed through a 500-ml nylon-coated titanium reactor at flow rates of 0.01 to 10 ml min(sup-1). the pressure in the reactor is controlled by a backpressure regulator with greater than 1% accuracy. in t ... | 1996 | 16535311 |
| clustering of marine bacteria in seawater enrichments. | seawater enrichments of marine bacteria clustered in 20- to 50-(mu)m-wide bands near air-water interfaces. the cells within the band travelled at up to 212 (mu)m s(sup-1) and at an average speed of 163 (mu)m s(sup-1). mean cell speeds peaked mid-run at 187 (mu)m s(sup-1). at the end of the run, bacteria reversed direction rather than randomly reorienting. the duration of the stops during reversal was estimated at 18 ms, six to seven times shorter than that found in enteric bacteria. cells hundre ... | 1996 | 16535420 |
| spoilage bacteria of fresh broiler chicken carcasses. | studies were conducted to identify the bacteria responsible for spoilage of fresh broiler chicken carcasses and to characterize the off-odors these bacteria produce. broiler carcasses were collected from processing plants in the northeast georgia area, the southeastern u.s., arkansas, california, and north carolina. the carcasses were allowed to spoil under controlled conditions at 3 c and spoilage bacteria were isolated. each spoilage bacterium was separately inoculated into a sterile chicken s ... | 1995 | 8825595 |
| homogentisic acid is the primary precursor of melanin synthesis in vibrio cholerae, a hyphomonas strain, and shewanella colwelliana. | the enzyme p-hydroxyphenylpyruvate hydroxylase (hpph) is involved in pigmentation (pyomelanin) via homogentisic acid (hga). pyomelanin formation is correlated with hga production and expression of hpph in three disparate marine species: vibrio cholerae, a hyphomonas strain, and shewanella colwelliana. induction of pigmentation in v. cholerae 569b by nutrient limitation also correlated with production of hga. | 1995 | 7747976 |
| phylogenetic analysis of the genera alteromonas, shewanella, and moritella using genes coding for small-subunit rrna sequences and division of the genus alteromonas into two genera, alteromonas (emended) and pseudoalteromonas gen. nov., and proposal of twelve new species combinations. | small-subunit ribosomal dna sequences were determined for 17 strains belonging to the genera alteromonas, shewanella, vibrio, and pseudomonas, and these sequences were analyzed by phylogenetic methods. the resulting data confirmed the existence of the genera shewanella and moritella, but suggested that the genus alteromonas should be split into two genera. we propose that a new genus, the genus pseudoalteromonas, should be created to accommodate 11 species that were previously alteromonas specie ... | 1995 | 7547295 |
| occurrence of a furan fatty acid in marine bacteria. | a fatty acid containing a furan ring was detected in the cellular lipids of marine bacteria, shewanella putrefaciens, marinomonas comunis, enterobacter agglomerans, pseudomonas fluorescens, etc., which were isolated from the intestinal liquor of fishes. analytical data indicated that the fatty acid was 10,13-epoxy-11-methyloctadeca-10, 12-dienoic acid. therefore, we propose that furan fatty acids detected in marine fish are derived not only from marine plants but also from intestinal bacteria of ... | 1995 | 7548190 |
| [the neferm test in the identification of psychrotrophic bacteria isolated from milk and dairy products]. | sanitary and nutriti on quality of foods from animal sources, that means also of milk and dairy products, are significantly influenced by the presence of psychrotrophic bacteria, the lipolytic and proteolytic activity of which contributes to their nutritive and sensory changes. this applies particularly to psychrotrophic bacteria of the genus pseudomonas, shewanella, alcaligenes and flavobacterium (muir and phillips, 1984; piton and richard, 1985; craven and macauley, 1990). these bacteria cause ... | 1995 | 7571245 |
| molecular cloning, expression in streptomyces lividans, and analysis of a gene cluster from arthrobacter simplex encoding 3-ketosteroid-delta 1-dehydrogenase, 3-ketosteroid-delta 5-isomerase and a hypothetical regulatory protein. | the arthrobacter simplex gene coding for 3-ketosteroid-delta 1-dehydrogenase, a key enzyme in the degradation of the steroid nucleus, was cloned in streptomyces lividans. nucleotide sequence analysis revealed that the gene for 3-ketosteroid-delta 1-dehydrogenase (ksdd) is clustered with at least two more genes possibly involved in steroid metabolism. upstream of ksdd, we found a gene, ksdr, encoding a hypothetical regulatory protein that shows homologies to kdgr, the negative regulator of pectin ... | 1995 | 7596291 |
| shewanella (pseudomonas) putrefaciens bacteremia. | shewanella (pseudomonas) putrefaciens is a rare pathogen in humans, and to our knowledge only 13 cases of s. putrefaciens bacteremia have ever been reported in the literature. in this retrospective study we describe 28 cases of s. putrefaciens bacteremia: 16 in premature and 1-day-old neonates, 9 in adults, and 3 in children younger than 1 year of age. all the babies presented with respiratory distress and/or pneumonia. six of the adults had associated traumatic lesions of the lower extremity, a ... | 1995 | 7620019 |
| cloning and expression of a gene encoding a t-cell reactive protein from coccidioides immitis: homology to 4-hydroxyphenylpyruvate dioxygenase and the mammalian f antigen. | the gene which encodes a previously described t-cell reactive protein (tcrp) of the human fungal pathogen coccidioides immitis (ci) was cloned and sequenced. both the genomic and cdna sequences were determined. the transcription start point was confirmed. the tcrp gene has three introns and a 1197-bp orf which translates to a 399-amino-acid (aa) protein (45.2 kda). the predicted protein has approx. 50% aa sequence identity and 70% similarity to mammalian 4-hydroxyphenylpyruvate dioxygenase (hppd ... | 1995 | 7642122 |
| modelling of microbial activity and prediction of shelf life for packed fresh fish. | prediction of shelf life based on growth of specific spoilage organisms (sso) in model substrates was studied. the effect of co2 on the growth kinetics for photobacterium phosphoreum and shewanella putrefaciens was quantified and modelled. results showed that microbial spoilage of packed cod stored with various concentrations of co2 was accurately predicted from the effect of co2 on p. phosphoreum grown in model substrates. the short shelf life extensions previously reported for packed cod there ... | 1995 | 7488526 |
| qualitative and quantitative characterization of spoilage bacteria from packed fish. | the large cells recently suggested to be responsible for spoilage of packed cod, have been identified as photobacterium phosphoreum. the spoilage activity of these cells, of shewanella putrefaciens and of other microorganisms isolated form spoiled packed cod has been studied. both qualitative and quantitative tests were used for characterization of the microbial spoilage activity. the importance of the different groups of microorganisms was evaluated by comparison of microbial spoilage activity ... | 1995 | 7488527 |
| antibacterial effect of protamine assayed by impedimetry. | impedimetric measurements were used to assay the antibacterial effect of protamine. a good linear correlation between the impedance detection time and the initial cell counts was obtained (r = 0.99, n = 2). as basic peptides may cause clumping of cells, this correlation curve was used when estimating the cell number after protamine treatment, rather than colony counts. protamine from salmon killed growing gram-positive bacteria and significantly inhibited growth of gram-negative bacteria in tryp ... | 1995 | 7730206 |
| spectroscopic and kinetic studies of the tetraheme flavocytochrome c from shewanella putrefaciens ncimb400. | electron paramagnetic resonance (epr) and magnetic circular dichroism (mcd) spectroscopic studies were carried out on the tetraheme flavocytochrome c from shewanella putrefaciens ncimb400. the epr spectrum reveals two sets of g-values--gz = 2.93, gy = 2.28, and gx = 1.51; and gz = 3.58--and the mcd spectrum shows a charge-transfer band at 1510 nm. these data combined show that all four hemes are low spin and have a nitrogenous sixth ligand. sequence comparisons with other tetraheme cytochromes, ... | 1995 | 7742319 |
| effects of electron donor and acceptor conditions on reductive dehalogenation of tetrachloromethane by shewanella putrefaciens 200. | shewanella putrefaciens 200 is a nonfermentative bacterium that is capable of dehalogenating tetrachloromethane to chloroform and other, unidentified products under anaerobic conditions. since s. putrefaciens 200 can respire anaerobically by using a variety of terminal electron acceptors, including no3-, no2-, and fe(iii), it provides a unique opportunity to study the competitive effects of different electron acceptors on dehalogenation in a single organism. the results of batch studies showed t ... | 1995 | 7887629 |
| purification and characterization of the 3-ketosteroid-delta 1-dehydrogenase of arthrobacter simplex produced in streptomyces lividans. | the 3-ketosteroid-delta 1-dehydrogenase (ks1dh) gene of arthrobacter simplex ifo12069 cloned in streptomyces lividans was overexpressed, resulting in production of the enzyme both extracellularly and intracellularly. the enzyme was purified by ammonium sulfate fractionation and chromatographies using deae-toyopearl, butyl-toyopearl and toyopearl hw55s from the supernatant of culture broth and cell-free extracts of s. lividans, and both preparations showed the same characteristics. the n-terminal ... | 1995 | 8586617 |
| dissolution and reduction of magnetite by bacteria. | magnetite (fe3o4) is an iron oxide of mixed oxidation state [fe(ii), fe(iii)] that contributes largely to geomagnetism and plays a significant role in diagenesis in marine and freshwater sediments. magnetic data are the primary evidence for ocean floor spreading and accurate interpretation of the sedimentary magnetic record depends on an understanding of the conditions under which magnetite is stable. though chemical reduction of magnetite by dissolved sulfide is well known, biological reduction ... | 1995 | 11539843 |
| anaerobic electron acceptor chemotaxis in shewanella putrefaciens. | shewanella putrefaciens mr-1 can grow either aerobically or anaerobically at the expense of many different electron acceptors and is often found in abundance at redox interfaces in nature. such redox interfaces are often characterized by very strong gradients of electron acceptors resulting from rapid microbial metabolism. the coincidence of s. putrefaciens abundance with environmental gradients prompted an examination of the ability of mr-1 to sense and respond to electron acceptor gradients ... | 1995 | 11536689 |
| the 16s rrna sequence and genome sizing of tributyltin resistant marine bacterium, strain m-1. | the 16s rrna of the tributyltin resistant marine bacterium, strain m-1, was partly sequenced to confirm the taxonomic status. the results indicated that this bacterium should be classified under the genus alteromonas, instead of a previous report in which this strain was identified as a vibrio. the genome size of this strain was also measured by pulsed field gel electrophoresis (pfge) using a contoureclamped homogeneous electric field. the strain was found to contain a genome size of 2,240 kilo ... | 1994 | 7512189 |
| effects of electron acceptors and donors on transformation of tetrachloromethane by shewanella putrefaciens mr-1. | transformation of chlorinated aliphatic compounds was examined in shewanella putrefaciens strain mr-1, an obligately respiring facultative anaerobe. under anaerobic conditions, mr-1 has been shown to transform tetrachloromethane to trichloromethane (24%), co2 (7%), cell-bound material (50%) and unidentified nonvolatile products (4%). the highest rate and extent of transformation were observed with mr-1 cells grown under iron(iii)-respiring conditions. lactate, formate and hydrogen were the most ... | 1994 | 7926693 |
| homogentisic acid is the product of mela, which mediates melanogenesis in the marine bacterium shewanella colwelliana d. | shewanella colwelliana d is a marine procaryote which produces a diffusible brown pigment that correlates with mela gene expression. previously, mela had been cloned, sequenced, and expressed in escherichia coli; however, the reaction product of mela had not been identified. this report identifies that product as homogentisic acid, provides evidence that the pigment is homogentisic acid-melanin (pyomelanin), and suggests that mela is p-hydroxyphenylpyruvate hydroxylase. this is the first report ... | 1994 | 8085836 |
| purification and properties of a novel cytochrome: flavocytochrome c from shewanella putrefaciens. | the major soluble cytochrome isolated from microaerobically grown cells of shewanella putrefaciens has been shown to be a novel type of flavocytochrome with fumarate reductase activity. this flavocytochrome, located in the periplasmic fraction of cell extracts, has been purified to homogeneity and shown to contain 4 mol of haem c and 1 mol of non-covalently bound fad per mol of protein. an m(r) value of 63,800 is estimated from sequence analysis assuming 4 mol of haem/mol of protein. in the pres ... | 1994 | 8093012 |
| sequence determination and mutational analysis of the lly locus of legionella pneumophila. | the lly (legiolysin) locus codes for a 39-kda protein which confers hemolysis, pigment production, and fluorescence on recombinant escherichia coli k-12 clones carrying the lly gene. the nucleotide sequences of the lly genes from two legionella pneumophila isolates were determined. the lly loci exhibited identical nucleotide sequences. they contained open reading frames of 348 amino acid residues, encoding proteins with a deduced molecular mass of 38.9 kda. n-terminal amino acid sequencing furth ... | 1994 | 8112844 |
| isolation of anaerobic respiratory mutants of shewannella putrefaciens and genetic analysis of mutants deficient in anaerobic growth on fe3+. | a genetic approach was used to study (dissimilatory) ferric iron (fe3+) reduction in shewanella putrefaciens 200. chemical mutagenesis procedures and two rapid plate assays were developed to facilitate the screening of fe3+ reduction-deficient mutants. sixty-two putative fe3+ reduction-deficient mutants were identified, and each was subsequently tested for its ability to grow anaerobically on various compounds as sole terminal electron acceptors, including fe3+, nitrate (no3-), nitrite (no2-), m ... | 1994 | 8113188 |
| ferric iron reduction-linked growth yields of shewanella putrefaciens mr-1. | the anaerobic reduction of ferric citrate by shewanella putrefaciens mr-1 cells was inhibited markedly by p-chloromercuriphenylsulphonate, moderately by potassium cyanide, and to a small extent by 2-heptyl-4-hydroxyquinolone-n-oxide. iron reduction was accompanied by increases in total cellular protein, with values of 0.33-7.54 g cell protein produced per mol fe(iii) reduced. the growth yields were dependent upon the growth conditions of the inoculum and the initial concentration of fe(iii) citr ... | 1994 | 8157545 |
| a biochemical study of the intermediary carbon metabolism of shewanella putrefaciens. | cell extracts were used to determine the enzymes involved in the intermediary carbon metabolism of several strains of shewanella putrefaciens. enzymes of the entner-doudoroff pathway (6-phosphogluconate dehydratase and 2-keto-3-deoxy-6-phosphogluconate aldolase) were detected, but those of the embden-meyerhof-parnas pathway were not. while several tricarboxylic acid cycle enzymes were present under both aerobic and anaerobic conditions, two key enzymes (2-oxoglutarate dehydrogenase and pyruvate ... | 1994 | 8195102 |
| the fnr family of transcriptional regulators. | homologues of the transcriptional regulator fnr from escherichia coli have been identified in a variety of taxonomically diverse bacterial species. despite being structurally very similar, members of the fnr family have disparate regulatory roles. those from shewanella putrefaciens, pseudomonas aeruginosa, pseudomonas stutzeri and rhodopseudomonas palustris are functionally similar to fnr in that they regulate anaerobic respiration or carbon metabolism. four rhizobial proteins (from rhizobium me ... | 1994 | 7747934 |
| the protein encoded by the shewanella colwelliana mela gene is a p-hydroxyphenylpyruvate dioxygenase. | the identity of the product of the mela gene from shewanella colwelliana with the enzyme p-hydroxyphenylpyruvic dioxygenase is shown. cloning of the mela gene endowed escherichia coli with the capacity to synthesize melanin-like pigments from l-tyrosine. e. coli contained transaminases that transforms l-tyrosine into p-hydroxyphenylpyruvate. this keto acid was detected in the cultures. on the other hand, e. coli containing mela was able to go further in the catabolic pathway, releasing a great a ... | 1994 | 7813886 |
| iron and manganese in anaerobic respiration: environmental significance, physiology, and regulation. | dissimilatory iron and/or manganese reduction is known to occur in several organisms, including anaerobic sulfur-reducing organisms such as geobacter metallireducens or desulfuromonas acetoxidans, and facultative aerobes such as shewanella putrefaciens. these bacteria couple both carbon oxidation and growth to the reduction of these metals, and inhibitor and competition experiments suggest that mn(iv) and fe(iii) are efficient electron acceptors similar to nitrate in redox abilities and capable ... | 1994 | 7826009 |
| [a simple method for differentiation of hydrogen sulfide-producing bacteria by the ph-dependent edta-sensitivity test]. | the ph-dependent edta-sensitivity test was performed to differentiate several strains of bacteria forming black colonies by the production of hydrogen sulfide on tcbs (thiosulfate-citrate-bile salt-sucrose agar) medium (tentatively designated as hydrogen-sulfide production bacteria). two halotorelant strains of 16 hydrogen sulfide-producing strains showed the same bacteriological properties and isoprenoid quinone type as did a reference strain of proteus mirabilis and were classified into the ed ... | 1994 | 7830320 |
| siderophore-mediated iron sequestering by shewanella putrefaciens. | the iron-sequestering abilities of 51 strains of shewanella putrefaciens isolated from different sources (fish, water, and warm-blooded animals) were assessed. thirty strains (60%) produced siderophores in heat-sterilized fish juice as determined by the chrome-azurol-s assay. all cultures were negative for the catechol-type siderophore, whereas 24 of the 30 siderophore-producing strains tested positive in the csáky test, indicating the production of siderophores of the hydroxamate type. sideroph ... | 1994 | 16349298 |