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dengue fever. reappearance in northern queensland after 26 years.during march, 1981, a number of cases of dengue fever occurred in cairns and townsville, northern queensland. from october, 1981, an outbreak of the infection was recognized on thursday island and, by may 1982, an estimated 38% of residents had been infected. isolated cases were reported from other towns in northern queensland and from other islands in the torres strait. clinical presentation varied from that of severe incapacitating illness lasting up to seven days to infections which were conf ...19846700459
bioassay of solubilized bacillus thuringiensis var. israelensis crystals by attachment to latex beads.solubilized crystals of bacillus thuringiensis var. israelensis were 7000 times less toxic to aedes aegypti larvae than intact crystals, presumably because mosquito larvae are filter feeders and selectively concentrate particles while excluding water and soluble molecules. a procedure is described whereby soluble toxins are adsorbed to 0.8-micrometer latex beads, with retention of toxicity. the latex bead assay should make it possible to analyze the structure and mode of action of the mosquito t ...19846701520
a large transmissible plasmid is required for crystal toxin production in bacillus thuringiensis variety israelensis.bacillus thuringiensis var. israelensis (bti), serotype 14, which produces parasporal crystals toxic to certain dipteran larvae, was analyzed by agarose gel electrophoresis and found to contain a complex plasmid array. eight plasmids were detected, with approximate sizes of 3.3, 4.2, 4.9, 10.6, 68, 75, 105, and 135 mda, as well as a plasmidlike linear dna element of approximately 10 mda. partially cured mutants of bti implicated the 75-mda plasmid in crystal production. fifteen independently iso ...19846709732
the effect of humidity on the transmission of brugia pahangi infective larvae to mammalian hosts by aedes aegypti.the transmission of brugia pahangi by aedes aegypti to the mammalian host was compared at low and high humidity. there was no statistical difference between the number of infected mosquitoes feeding or the egress of infective larvae from these mosquitoes at high or low humidity. the penetration of the host by the infective larvae was however significantly greater (p less than 0.05) at high than at a low humidity.19846710572
plasmodium gallinaceum: erythrocyte factor essential for zygote infection of aedes aegypti.zygotes of plasmodium gallinaceum, fertilized in vitro and fed to aedes aegypti mosquitoes through a membrane, formed oocysts only when a substance in the cytoplasm of uninfected erythrocytes was present. the relation between erythrocyte volume and infectivity was linear (1:1.2) up to a 50% hematocrit. the intraerythrocytic substance was both nondialyzable and poorly soluble in plasma. by carboxymethyl cellulose chromatography, cytoplasmic constituents eluted at ph 8.6 supported the same infecti ...19846714357
[comparison of the flight capability of plasmodia-infected and plasmodia-free aedes aegypti]. 19846717377
the use of mosquitoes (aedes aegypti) to detect ciguatoxin in surgeon fishes (ctenochaetus striatus).a new animal assay to detect ciguatoxin in fishes was developed. mosquitoes were subjected to intrathoracic injection of serial dilutions of ciguatoxin crude extracts. toxicity of the fishes was expressed as the mosquito ld50 (g of flesh per mosquito). a significant correlation between the mosquito bioassay and the mouse bioassay performed on the same extracts was shown (r = 0.72, n = 80). we were able to detect 96% of the toxic fishes, while 91% of the fishes non-toxic in mice were also non-tox ...19846719474
a mosquito-virulent bacillus sphaericus in adult simulium damnosum from northern nigeria.from newly emerged adult simulium damnosum from kaduna river, northern nigeria, a mosquito-virulent strain of bacillus sphaericus was isolated. in another four cases non-virulent b. sphaericus were present. the isolate exerted a lc50 of 50 spores/ml for culex pipiens l4-larvae and was less virulent for anopheles stephensi (400 sp/ml) and aedes aegypti (800 sp/ml).19846720123
plasmodium gallinaceum: density dependent limits on infectivity to aedes aegypti.in acute, blood-induced infections of chickens, the malarial parasite plasmodium gallinaceum is most infective to the mosquito aedes aegypti 1 day before gametocyte numbers peak. in an effort to account for this disynchrony , daily changes in parasite infectivity, parasitemia, hematocrit, and hemoglobin were measured during the course of infections. three events were correlated with the loss of infectivity: (1) in the 24 hr between park infectivity and peak gametocytemia , schizont-induced hemol ...19846723894
[ecology of dengue and aedes aegypti. preliminary research. 2]. 19846729600
genetic heterogeneity among caribbean populations of aedes aegypti.using starch gel electrophoresis, we have analyzed genetic variation at 11 loci in 18 caribbean collections of aedes aegypti. our results show that, while there is some relationship between geographic proximity and genetic distance, the overall picture among islands is one of gene frequency patchiness , with some collections clearly not conforming to any geographic pattern. we attribute this to the combined effects of high rates of gene flow among islands and with the mainland american continent ...19846731681
experimental aerial spraying with ultra-low-volume (ulv) malathion to control aedes aegypti in buga, colombia. 19846733345
blood feeding affects age-related changes in the host-seeking behavior of aedes aegypti (diptera: culicidae) during oocyte maturation. 19846748001
in vitro cultivation of infective larvae of dirofilaria immitis.infective larvae (l3) of dirofilaria immitis were cultivated in various synthetic media. l3 obtained from aedes aegypti mosquitoes by mass dissection were inoculated into the media, viz: rpmi 1640 medium with 10 per cent human ab serum (medium-a); tc-199 medium with organic acids, sugars of grace's insect medium and 10 per cent foetal calf serum (fcs) (medium-b); and grace's insect medium with 10 per cent fcs (medium-c). maximum survival of 26 days was found in medium 'a'. approximately 80 per c ...198412055792
the inactivation of acetylcholinesterase by alpha-terthienyl and ultraviolet light. studies in vitro and in larvae of the mosquito aedes aegypti.acetylcholinesterase (ec 3.1.1.7) was inactivated photochemically in solution, in the presence of dissolved terthiophene sensitizers. alpha-terthienyl (2,2':5,2"-terthiophene) and its isomers 3,2':5',2"- and 3,2':5',3"-terthiophenes showed very similar sensitizing properties. with all three terthiophenes, the photosensitization was completely suppressed under anaerobic conditions, and therefore the inactivation process required the presence of oxygen. the enzyme was inactivated in vivo when four ...19846509086
genetic variation in an urban population of aedes aegypti (diptera: culicidae). 19846502629
amino acids and peptides. xi. synthesis of attractant and repellent peptides for aedes aegypti and blattella germanica. 19846532560
paradoxical effects of simulated larviciding on production of adult mosquitoes.we determined whether simulated mortality of larval mosquitoes in food-deprived bodies of water increases emergence of adult females as well as their size by releasing the remaining larvae from competition for food. removal of larval aedes aegypti from rearing containers with suboptimal amounts of food increased the number of adults that emerged. thus, larvicidal measures do not inevitably lead to reduced numbers of mosquito vectors of disease. in food-limiting breeding sites, partially effectiv ...19846507734
quantitative structure-activity relationship of insect juvenile hormone mimetic compounds.juvenile hormone mimetic activities on aedes aegypti (yellow-fever mosquito) and tenebrio molitor (yellow mealworm) of compounds having (2e,4e)-3,7,11-trimethyl-2,4-dodecadienone structures were comparatively and quantitatively analyzed in terms of their physiochemical structural parameters and by regression analysis. they were structurally composed of three classes, ester and thiol ester derivatives, amides, and ketones, depending on the c1 substituents. the results indicated that the steric di ...19846492079
hormonal regulation of ovarian ecdysteroid production in the autogenous mosquito, aedes atropalpus.the effects of juvenile hormone (jh) and egg development neurosecretory hormone (ednh) on ovarian ecdysteroid production during vitellogenesis in the autogenous mosquito, aedes atropalpus, were investigated using in vitro techniques coupled with radioimmunoassay (ria) and high-pressure liquid chromatography (hplc). normal females were characterized by quantitative, qualitative, and temporal patterns of in vitro ovarian ecdysteroid production. females decapitated at emergence showed little ovaria ...19846541607
esterase activity in strains of aedes aegypti (diptera: culicidae) tolerant and susceptible to the organophosphate insecticide malathion. 19846492084
lithium attenuates the active transport of calcium in the larva of aëdes aegypti. 19846491591
the toxic effects of triorganotin(iv) compounds on the culicine mosquito, aedes aegypti (l).the structure-activity relationships of 25 triorganotin(iv) compounds were studied using the 4th instar a. aegypti larvae of the susceptible liverpool red-eye strain. the lc50 values of these compounds were compared with those of malathion, ddt and temephos. maximal activities were obtained with tritolyltin chloride and its complexes with triphenyl-phosphine oxide and pyridine n-oxide, dimethyloctyltin- and diethyloctyltin acetates and tributyltin sucrose phthalate. a limited number of the trior ...19846204424
effects of toxorhynchites amboinensis on laboratory-reared aedes aegypti populations (diptera: culicidae). 19846144797
salivary apyrase of aedes aegypti: characterization and secretory fate.salivary gland homogenates of female adult aedes aegypti hydrolyzed atp and adp thereby defining an apyrase activity. activity is divalent cation dependent with an optimum ph of 9.0. atpase and adpase activities could not be dissociated thus suggesting the presence of a true apyrase enzyme. apyrase activity is low on the day of emergence but increases to 160 mu per pair of glands on the second day. the site at which mosquitoes probed into warm polyacrylamide gels retains apyrase activity, confir ...19846094095
cryopreservation of the microfilariae of brugia malayi, dirofilaria corynodes, and wuchereria bancrofti.methods are presented for the cryopreservation of a sheathed microfilaria, brugia malayi, and an unsheathed species, dirofilaria corynodes. the former survived best when frozen at the rate of -0.8 degree or -0.5 degree c per minute using 9% dimethyl sulfoxide (dmso) as the cryopreservative. approximately 52-79% of the thawed microfilariae developed to the third stage in aedes aegypti mosquitoes versus 79% of the unfrozen specimens. for d. corynodes the optimum freezing rate was -2 degrees or -5 ...19836824119
epidemiologic investigations of dengue infection in mexico, 1980.a binational investigation was conducted in two mexican cities in 1980 to study epidemiologic characteristics of dengue. two study areas were selected in each of the cities (merida and tampico); in each area, in february and in september, sanitarians recorded information concerning abundance of aedes aegypti, and public health nurses obtained blood specimens and clinical information from residents. ninety-nine individuals (24% of the study population) showed serologic evidence of recent dengue 1 ...19836829561
plasmodium gallinaceum: avian screen for drugs with radical curative properties.existing primary screens for radical curative antimalarial drugs fail to adequately detect many compounds which affect the latent, exoerythrocytic hypnozoite, the stage of the parasite responsible for relapse. at the same time, these screens falsely identify a wide range of compounds with no radical curative activity. the avian malaria, plasmodium gallinaceum, and aedes aegypti mosquitos were used in a screen which measures the effects of candidate compounds on gametocytes and their development ...19836832279
larvicidal activity of bacillus thuringiensis subsp. israelensis, serovar h14 in aedes aegypti: histopathological studies. 19836841993
[repellent properties of mannich bases and cresol and phenol derivatives for aedes aegypti l. mosquitoes and the flea xenopsylla cheopis roths]. 19836843496
[effect of the larval density of aedes aegypti mosquitoes on their mortality as affected by bacillus thuringiensis berl. preparations]. 19836843501
transovarial transmission of dengue 2 virus by aedes aegypti in nature.dengue 2 virus was recovered from three of 123 pools of naturally infected aedes aegypti larvae (6,200 insects) collected from water containers in rangoon. the virus was also isolated from two of 76 pools (7,730 mosquitoes) of male ae. aegypti, collected as larvae and reared in the laboratory to adults. minimum field infection rates among these two groups of mosquitoes were 1:2,067 and 1:3,865, respectively. insect pools were inoculated into toxorhynchites splendens mosquitoes and dengue viral a ...19836859404
[action of crystals of bacillus thuringiensis var. israelensis on the midgut of aedes aegypti l. larvae, studied by electron microscopy].ingestion of bacillus thuringiensis var. israelensis crystals by aedes aegypti larvae is followed by midgut epithelium disruption. earliest ultrastructural changes consist of an enlargement of intra- and intercellular spaces in the basal region of the cell. endoplasmic reticula disintegrate by forming spherical structures which increase in size during intoxication. mitochondria are transformed at first into a condensed form, then become swollen with the disappearance of internal cristae. in the ...19836870088
toxicity of bacillus thuringiensis var. israelensis crystals to aedes aegypti larvae: carbonate reversal.the toxicity of purified bacillus thuringiensis var. israelensis crystals to larvae of aedes aegypti could be reversed 100-fold by levels of k(2)co(3) as low as 0.15%.19836870244
effect of trypsin and neuraminidase on the infectivity of plasmodium gallinaceum sporozoites.an investigation was carried out to examine the effect of trypsin and neuraminidase on the infectivity of plasmodium gallinaceum sporozoites. the sporozoites were obtained from freshly dissected salivary glands of aedes aegypti: their infectivity was assayed by their ability to produce a patent parasitaemia following their inoculation into day-old chicks. infectivity was abolished by treatment of the sporozoites with trypsin at a concentration of 100 micrograms ml-1 for 30 minutes at 30 degrees ...19836882061
the regulation of preimaginal populations of aedes aegypti l. (diptera: culicidae) on the kenya coast. i. preimaginal population dynamics and the role of human behaviour.on the kenya coast aedes aegypti breeds in pots maintained indoors for water storage. pupal numbers vary greatly from one pot to another. the rhythm of water replenishment and the accidental introduction of food into the pots regulate pupal numbers. when water replenishment occurs more often than once per week, pupal numbers are small. when replenishment occurs less often, both low and high pupal numbers may be observed. the presence or absence of larval food distinguishes pots of high versus lo ...19836882066
production of bacillus sphaericus strain 1593 primary powder on media made from locally obtainable nigerian agricultural products.five media, formulated from dried cow blood, mineral salts, and seeds from four species of legumes, were assessed for growth, sporulation, and insecticidal properties of bacillus sphaericus strain 1593. bacterial powders, prepared from broth, were assayed against culex quinquefasciatus, anopheles gambiae, and aedes aegypti. good growth and sporulation were obtained with all the media. the highest number of viable cells and spores per mililitre (8.6 x 10(8) and 8.1 x 10(8] were obtained in media ...19836883226
macrogeographic genetic variation in a human commensal: aedes aegypti, the yellow fever mosquito. 19836884770
development of dirofilaria repens in aedes aegypti reared in contrasting habitat.the susceptibility of dirofilaria repens infection in aedes aegypti adults reared in hay-infusion and tap-water during their larval stages was tested. ae. aegypti reared in hay-infusion ingested a bigger amount of blood than those reared in tap-water but harboured fewer d. repens larvae (developing and arrested). the difference in the susceptibility of mosquitoes from the two treatments was correlated with the difference in the robustness of the mosquitoes.19836612416
breeding of aedes aegypti in closed septic tanks. 19836618537
transovarial transmission of dengue viruses by mosquitoes: aedes albopictus and aedes aegypti.transovarial transmission of all four dengue serotypes was demonstrated in aedes albopictus mosquitoes. the rates of such transmission varied with the serotype and strain of virus. in general, the highest rates were observed with strains of dengue type 1 and the lowest with dengue type 3. surprisingly, despite the use of viral strains of the four dengue serotypes which gave the highest rates with ae. albopictus, transovarial transmission was observed in aedes aegypti only with dengue type 1, and ...19836625066
toxicity of bacillus thuringiensis subsp. israelensis to adult aedes aegypti mosquitoes.adult female aedes aegypti mosquitoes were killed by the parasporal crystals of bacillus thuringiensis subsp. israelensis (onr-60a) when the crystals were introduced into the insect midgut as an enema. the 50% lethal dose for intact parasporal crystals was 0.21 microgram/mg of mosquito (wet weight), and for solubilized crystals the 50% lethal dose was 0.04 microgram/mg. these values were compared with 50% lethal concentrations in a free-feeding larval mosquito bioassay of 0.018 and 1.28 microgra ...19836625566
the problems of aedes aegypti control in the americas. 19836626807
[diseases transmitted by mosquitoes and urbanization. examples of urban vectors of dengue and filariasis].once located in time and place the beginning of urbanization process, the mechanisms of adaptation to urban ecosystem and of dissemination by human conveyances are studied for two domestic mosquitoes: aedes aegypti and culex pipiens fatigans (= quinque-fasciatus). then epidemiological consequences are discussed. ae. aegypti pullulation constitutes a potential risk of urban yellow fever outbreak and the main factor of dengue haemorrhagic fever appearance; the increase of c. p. fatigans population ...19836627544
monoclonal antibodies against surface determinants on gametes of plasmodium gallinaceum block transmission of malaria parasites to mosquitoes.monoclonal antibodies (mab) against gametes of the chicken malaria plasmodium gallinaceum have been derived. all reacted with the surface of extracellular gametes of the parasite in immunofluorescent antibody reactions and all agglutinated both male and female gametes. in the absence of active complement one mu isotype mab, la 1-d5, mediated at least 95% suppression of infectivity of the parasites to aedes aegypti mosquitoes. individually, mab of the gamma 1 or gamma 2a isotypes mediated only sl ...19836631012
[action of the delta-endotoxin of bacillus thuringiensis var. israelensis on cultured cells from aedes aegypti l].the effects of bacillus thuringiensis var. israelensis delta-endotoxin were investigated on a cell culture of aedes aegypti with the electron microscope. the ultrastructural changes following intoxication were: disintegration of endoplasmic reticula by the formation of spherical structures; condensation and then swelling of mitochondria with disappearance of internal cristae; frequent dilatation of the perinuclear space; cellular lysis. the action of a metabolic inhibitor, 2,6-dibromophenol (2,6 ...19836638755
vertebrate host specificity and experimental vectors of plasmodium (novyella) kempi sp. n. from the eastern wild turkey in iowa.vertebrate host specificity, experimental laboratory vectors, and a description of plasmodium (novyella) kempi sp. n. from eastern wild turkeys (meleagris gallopavo silvestris vieillot) in iowa are presented. plasmodium kempi is infective for domestic turkeys, bobwhites (colinus virginianus), chukars (alectoris graeca), guinea fowl (numida meleagris), peacocks (pavo cristatus), and canaries (serinus canaria), produces a transient infection in mallards (anas platyrhynchos) and domestic geese (ans ...19836644918
transmission of dengue virus by orally infected aedes triseriatus.transmission of dengue type 1 was demonstrated for 3 strains of aedes triseriatus mosquitoes after oral infection. rates of infection were similar to those observed in a control strain of aedes aegypti. three additional species belonging to the subgenus protomacleaya (aedes brelandi, aedes hendersoni, and aedes zoosophus) were also susceptible to oral infection with dengue type 1 virus but transmission could not be demonstrated although virus was detected in the salivary glands of infected mosqu ...19836650743
a case study of aedes aegypti prevalence by settlement types in dehu town group of maharashtra state. 19836668009
linkage relationships of eleven enzyme loci in the aedes scutellaris group.linkage relationships of 11 enzyme loci were determined in backcrosses between aedes polynesiensis and aedes kesseli. three linkage groups established were aat2-lap2-me-sex, cat-ao-pgm-idh2-est6, and gpi-odh-pgd. lap2 and cat have not been previously mapped in aedes. locus order and linkage groups were the same as those observed for seven loci mapped in aedes aegypti. the significance of the observed similarities in chromosome organization and differences in crossover values among closely relate ...19836670992
susceptibility of aedes aegypti to infections with dirofilaria immitis and dirofilaria repens.eight stocks of aedes aegypti-4 geographic stocks and 4 selected stocks-were tested for their susceptibility to infection with dirofilaria immitis and d. repens. four of the stocks were found to be susceptible and the other 4 were refractory of infection with d. immitis. all the stocks of ae. aegypti that were susceptible to d. immitis were also susceptible to d. repens but the stocks that were refractory to d. immitis were all partially susceptible to d. repens.19836673132
characterization of antigens on mosquito midgut stages of plasmodium gallinaceum. i. zygote surface antigens.we have defined the surface protein antigens on plasmodium gallinaceum zygotes using radioiodination methods and rabbit anti-zygote serum which blocks transmission of the parasite to aedes aegypti mosquitoes. fifteen protein bands (1-15) in the molecular weight range of 40 000-240 000 and one band at the bromophenol blue dye marker were labelled by the lactoperoxidase and iodogen (1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril) methods. the localization of these radioiodinated components ...19836683783
cultivation of mosquito cell lines in serum-free media and their effects on dengue virus replication.seven mosquito cell lines from five species (aedes aegypti, ae. albopictus, ae. pseudoscutellaris, culex tarsalis, and toxorhynchites amboinensis) were adapted to three kinds of serum-free media (sem), which were composed of equal volumes of tryptose phosphate broth and of either leibovitz (l15) medium, eagle's minimum essential medium, or medium 199 with hanks' salts. population growth rates of the cells cultivated in the smfs were generally slower than those of original cell cultures maintaine ...19836137452
the biological control of aedes aegypti on sa-med island, rayong province by means of toxorhynchites splendens, a predatory mosquito larva. 19836138386
experimental model of transovarial transmission of tahyna virus in aedes aegypti mosquitoes.the progeny of 31 viruliferous aedes aegypti females infected with tahyna virus by sucking on viraemic newborn mice was investigated for virus presence. out of 1587 individuals of the f1 generation, 16 suspensions representing the progeny of 7 females were positive in 146 trials. individuals of the f1 generation failed to transfer the virus by sucking. electron microscopy revealed the presence of tahyna virus particles in the cytoplasm of maturing oocytes inevitably confirming the transovarial t ...19836138986
toxicity of cyclic peptide antibiotics to larvae of aedes aegypti. 19836196417
relative toxicity of organic solvents to aedes aegypti larvae. 19836631047
evaluation of a controlled-release silicate formulation of temephos against aedes aegypti larvae in the laboratory and psorophora columbiae larvae (diptera: culicidae) in rice field plots. 19836192242
peritrophic membranes and protease activity in the midgut of the malaria mosquito, anopheles stephensi (liston) (insecta: diptera) under normal and experimental conditions.formation and solubility of the peritrophic membranes (pm) in the midgut (stomach) of female anopheles stephensi depended on salt concentration in the gut lumen. high calcium drastically reduced the solubility of the pm in vitro. thin-layer chromatography revealed the presence of n-acetylgalactosamine and galactose in the pm. in contrast to aedes aegypti no n-acetylglucosamine was present indicating significant differences between anopheles and aedes mosquitoes with respect to pm formation and c ...19836345801
the exchange of calcium in larvae of the mosquito aedes aegypti.the exchange of labelled calcium between the external medium and the whole body was investigated in the larva of aedes aegypti (l.) using a closed, two-compartmental model. the transport system for the uptake of ca2+ was found to be saturable and obeyed michaelis-menten kinetics. the efficiency of the inward transport of calcium from dilute solutions was markedly reduced by starvation or by ruthenium-red, a selective inhibitor of ca2+ activated atpase, indicating that this transport system is en ...19836192197
insecticidal evaluation of zolone against culex quinque-fasciatus, anopheles culicifacies, a. stephensi and aedes aegypti. 19836137454
inheritance of brown-eye and colourless-eye in the mosquito aedes aegypti.an autosomal recessive gene bw (probably closely linked to y on chromosome 2) gives a brown eye colour. when this gene and the sex-linked gene re (red-eye) are both homozygous, a colourless-eye results. brown-eye shows good viability and penetrance, and it has been maintained as a pure stock. colourless-eye shows 87% penetrance in both sexes, and the females are low in viability and fertility. a colourless-eye stock has been maintained by crossing colourless-eyed males to their heterozygous red- ...19836882068
diuresis in newly emerged, unfed mosquitoes. ii. the basic pattern in relation to escape from the water, preparation for mature flight, mating and the first blood meal.a study of 2000 newly emerged adult mosquitoes (aedes aegypti) shows that the rate of excretion is high at the time of eclosion but falls rapidly over the next few minutes. in both sexes, however, the high rate of excretion is regained during a second peak in the middle of day 1. it remains high for about 6 h and then falls in a remarkably steady manner to reach a low level plain, which persists at least till the end of day 3. in females this plain is not reached till some 8 h after the onset in ...19836132392
an osmoregulatory syncytium and associated cells in a freshwater mosquito.in embryological terms the anal papillae are the product of eversion of the hindgut tissues. the rectum and the anal papillae have the same origin and have a marked structural similarity. the insect hindgut is very labile being able to produce salt transporting or 'chloride cells' from any of the tissues of which it is composed. the hindgut consists of four distinct regions: the ileum and part of the anal canal have a mechanical function, the rectum and the posterior anal canal contain transport ...19836879594
insecticidal effect of aliphatic alcohols against aquatic stages of aedes mosquitoes.effectiveness of certain long carbon chain aliphatic alcohols as ovicides and larvicides was investigated under laboratory conditions against eggs and larvae of aedes aegypti and aedes scutellaris. out of the eight alcohols tested, three (1-decanol, 1-undecanol and 1-dodecanol) proved to be very effective. the hatching of mosquito eggs was inhibited at a dosage of 5 to 7 litres/hectare. first, third and fourth instar larvae were killed at a dosage of 3 to 7 litres/hectare. first instar larvae we ...19836683011
[new regioselective synthesis of racemic 7,11-dimethyloctadecane--an ovipositon attractant for the yellow fever mosquito (aedes aegypti)]. 19836628194
previtellogenic development and vitellogenin synthesis in the fat body of a mosquito: an ultrastructural and immunocytochemical study.we describe two phases, previtellogenic and vitellogenic, in the activity of the trophocytes in the fat body of the mosquito aedes aegypti. the previtellogenic phase, leading to trophocyte competence to synthesize vitellogenin (vg), occurred during the first 3 days after eclosion. this phase was characterized by enlargement and activation of the nucleoli, proliferation of ribosomes and rough endoplasmic reticulum (rer), development of golgi complexes, and extensive invaginations of the plasma me ...19836349013
[reproduction of powassan and west nile viruses in aedes aegypti mosquitoes and their cell culture]. 19826289063
eradication of aedes aegypti on cayman brac and little cayman, west indies, with abate (temephos) in 1970-1971. 19826176286
role of the parasporal body in causing toxicity of bacillus thuringiensis toward aedes aegypti larvae. 19826120198
[study of the larvicidal activity of bacillus thuringiensis var. israelensis on toxorhynchitinae larvae (author's transl)].toxicity of bacillus thuringiensis var. israelensis, h 14 serotype, has been tested on larvae of toxorhynchites amboinensis, predaceous larvae of other culicinae larvae. the toxorhynchites larvae are not sensitive to the bacterial toxin up to relatively high dosages, except when larvae of aedes aegypti are provided in the suspension. mode of action of the crystal appears to be similar to the one observed in the epithelial cells of intoxicated aedes aegypti larvae midgut.19826125271
ingestion and development of wuchereria bancrofti in culex quinquefasciatus, anopheles gambiae and aedes aegypti after feeding on humans with varying densities of microfilariae in tanzania. 19826126022
comparative bioassays of bacillus thuringiensis h-14 formulations against four species of mosquitoes in malaysia.comparative laboratory bioassays of three formulations of bacillus thuringiensis h-14 (ips-78, san 402-i and bactimos) were conducted against late 3rd/early 4th instar larvae of four species of mosquito, viz., aedes aegypti, culex quinquefasciatus, anopheles balabacensis and mansonia (mansonioides) indiana, in malaysia. from the average response of the mosquito larvae to the three formulations of b. thuringiensis h-14, ae. aegypti was found to be most susceptible, followed by cx. quinquefasciatu ...19826128794
the effects of parabiotic twinning of susceptible and refractory mosquitoes on the development of plasmodium gallinaceum.two species of mosquitoes were joined parabiotically with glass capillaries so as to share common hemolymph. in experiments designed to determine optimum physical factors was found that capillaries of 2.5 mm in length, 100 microns od and with pointed ends were tolerated best by mosquitoes and permitted optimum hemolymph transfer. maximum survival of mosquitoes was noted when capillaries were inserted in the post mesospiracular membranous area, in the largest mosquito first and allowed to fill wi ...19826129293
nondevelopment of wuchereria bancrofti in nude (congenitally athymic) mice.infective larvae of wuchereria bancrofti (nematoda: filarioidea) were harvested from aedes aegypti mosquitoes fed in vitro on microfilaremic blood of haitian volunteers. larvae were inoculated either into the subcutaneous tissue (sc) or peritoneal cavity (ip) of congenitally athymic (nude) mice, c3h/hen (nu/nu). initially 15 male and two female mice received larval doses from 38-180, and necropsy was done between 11 and 75 days post-inoculation. no worms were found at necropsy. four additional m ...19826751107
comparative study of hemocytes and associated cells of some medically important dipterans.the aim of this work is to study, characterize, and compare different morphological types of hemocytes of glossina austeni, g. morsitans, calliphora erythrocephala, stomoxys calcitrans, lucilia sericata, aedes aegypti, and culex quinquefasciatus. this information is intended to provide a basis for future studies of the cellular defense mechanisms of these dipterans. seven morphological types of hemocytes were identified by phase-contrast optics: prohemocytes, plasmatocytes, thrombocytoids, granu ...19826764649
host behaviour and mosquito feeding success: an experimental study.the susceptibility of laboratory rabbits to attack by mosquitoes (aedes aegypti) is found to be closely linked to their grooming activity. prevention of grooming significantly increases both the percentage of mosquitoes feeding and the size of blood meals. grooming responses intensify with parasite density, and lead to a slight decline in parasite feeding success and blood meal size. grooming efficiency varied between individual rabbits and also a consequence of experience, with rabbits improvin ...19827080144
the infectivity of microfilariae of brugia pahangi of different ages to aedes aegypti.by transferring microfilariae of brugia pahangi which had been born over a 24-hour period in the peritoneal cavities of jirds (meriones unguiculatus) to the blood circulation of other jirds, infections of known age were tested for their ability to develop into third-stage larvae (l3) in mosquitoes aedes aegypti. microfilariae less than three days old were not able to develop to l3. microfilariae which had been in circulation for three days to six months were capable of developing if ingested.19827096971
[on an outbreak caused by dengue type 4 virus, in thio, new caledonia. epidemiological and clinical aspects (author's transl)].after imported cases, a dengue outbreak, caused by type 4 virus, affected 11 per cent of the habitants of thio, new caledonia. distribution of cases by sex, by ethnic and age group, by locality and by week is established on cases confirmed by serology and/or by virus isolation. clinical aspect was classical but liver and digestive tract of patients were frequently affected. five haemorrhagic forms were observed. aedes aegypti was found in each visited house; pooled specimens were found infected ...19827105285
infection of aedes aegypti with zygotes of plasmodium gallinaceum fertilized in vitro.female gametes of plasmodium gallinaceum fertilized in vitro, cleaned of all other blood constituents, resuspended in blood, and fed to aedes aegypti through a membrane were infective. at the lowest zygote concentration, 10(4)/ml, nearly every ingested parasite produced an oocyst. as the concentration ingested increased, efficiency to infect diminished, until above 10(7) zygotes/ml the number of oocysts produced became constant. this method should be valuable for determining the nutrient require ...19827119992
estimation of the amount of venezuelan equine encephalomyelitis virus transmitted by a single infected aedes aegypti (diptera: culicidae). 19827120306
dengue-2 vaccine: infection of aedes aegypti mosquitoes by feeding on viremic recipients.colonized aedes aegypti mosquitoes were fed on voluntary recipients of an experimental, live, attenuated, dengue type 2 (pr 159/s-1) vaccine to estimate the frequency of vector infection and the stability of the virus in mosquitoes. two volunteers were viremic at the time of mosquito feeding, but only two of 114 mosquitoes that took a viremic blood meal became infected with the vaccine virus. strains of virus recovered from the bodies of the mosquitoes and the volunteer's blood retained the temp ...19827149107
dengue-2 vaccine: oral infection, transmission, and lack of evidence for reversion in the mosquito, aedes aegypti.the dengue-2 vaccine virus (s-1), and its parent virus (pr-159), were compared for their ability to infect orally, to replicate in, and subsequently to be transmitted by aedes aegypti mosquitoes. the vaccine virus was markedly less efficient in its ability to infect mosquitoes orally. after ingesting infectious bloodmeals containing 3, 7 to 8.2 log10mid50/ml of the respective viruses, 56% (220/396) of the mosquitoes became orally infected with the parent virus contrasted with 16% (66/397) for th ...19827149108
laboratory evaluation of bacillus thuringiensis var. israelensis serotype h-14 against mosquito larvae with particular reference to aedes aegypti. 19827153478
transmission of venezuelan equine encephalomyelitis virus by aedes aegypti (diptera: culicidae) to mice previously exposed to vector antigens. 19827154017
a kinetic and ultrastructural comparison of alphavirus infection of cultured mosquito and vertebrate cells.vero cells and aedes pseudoscutellaris cells showed rapid production of semliki forest virus (sfv) whereas in aedes aegypti and anopheles stephensi cells no rapid production of sfv was observed. ultrastructurally the only virally induced cell inclusion in early infection was the cytopathic vacuole type 1. later in infection, in mosquito cells, electron-dense bodies appear and budding of new virions appears to be very efficient. in vero cells large accumulations of envelope proteins and nucleocap ...19827154145
well breeding behaviour of aedes aegypti. 19827166362
the ultrastructure of the larval malpighian tubules of a saline-water mosquito.the larval malpighian tubules of the saline-water mosquito aedes taeniorhynchus were examined using light and electron microscopy. the tubules contain two cell types; primary cells and stellate cells. primary cells are characterized by their size (70 microns x 70 microns x 10 microns) and an abundance of intracellular membrane-bound crystals. two types of microvilli are found on the luminal surface of the primary cells: (1) small microvilli containing core microfilaments and extensions of endopl ...19827170712
multiplication of coxiella burnetii in aedes aegypti. 19827174009
distribution and density of aedes aegypti (l) and aedes albopictus (skuse) in sarawak. 19827176997
[distribution of aedes aegypti in china and its comprehensive control]. 19827185486
[dipetalonema dessetae in proechimys oris. ii. evaluation of the model for pharmacologic investigations of antifilarial chemotherapy (author's transl)].dipetalonema dessetae in proechimys oris is a new model of rodent feafilariasis with several interesting features with respect to its utilisation in antifilarial pharmacology. 1. in the first part of these studies, it has been shown that the final host of the filaria, a rodent, was easy to breed. a prolific mosquito aedes aegypti was an effective vector, and with the selected mode of infection i.e. 200 infesting larvae subcutaneously injected, 80% of the rodents were infected. 2. from 90 to 150 ...19827200173
[intake of microfilariae by the vector in the case of a low microfilareamia (author's transl)].the study of the intake of microfilariae of dipetalonema dessetae by aedes aegypti leads to two conclusions with respect to the microfilaraemia: --there is no concentration of microfilariae in the uptake of blood by aedes. --the microfilariae are nearly homogeneously distributed in the vertebrate host blood available to the vector for feeding. such a distribution of microfilariae in the cutaneous blood supply of the host gives the maximum chance for a mosquito to become infected when taking a bl ...19827200752
melanization and encapsulation in aedes aegypti and aedes togoi in response to parasitization by a filarioid nematode (breinlia booliati).in aedes aegypti (singapore strain), s refractory host, there was a marked progressive decline of developing larvae of breinlia booliati during the incubation period. however, in aedes togoi, a susceptible host, the filarial larvae developed normally and the number of larvae remained constant throughout the incubation period. encapsulation and melanization of b. booliati larvae in ae. aegypti and ae. togoi were studied. ae. togoi occasionally mounted a defence reaction to the infection. in ae. a ...19827177704
field trials on the effectiveness of bioresmethrin reslin 10/10 (r) on aedes aegypti. 19827167094
an efficient floating larval trap for sampling aedes aegypti populations (diptera: culicidae). 19827154026
effects of benzylphenol and benzyl-1,3-benzodioxole derivatives on fertility and longevity of the yellow fever mosquito (diptera:culicidae). 19827175006
diuresis in newly emerged, unfed mosquitoes. i. fluid loss in normal females and males during the first 20 hours of adult life.unfed mosquitoes (aedes aegypti) decapitated soon after emergence from the pupa survive better than those decapitated later. in a test involving more than 700 females and more than 600 males, 80% of the mosquitoes decapitated during the first hour of adult life survived a period of 45 h post emergence (p.e.), whereas about 80% of those decapitated at 12 h or later failed to survive the same period. there was a linear fall in the rate of survival between 1 and 10 h p.e., after which the rate fluc ...19826129627
effect of toxorhynchites brevipalpis on aedes aegypti (diptera: culicidae) in continuous-breeding laboratory populations. 19826130156
geographic and temporal patterns of genetic variation of aedes aegypti in new orleans.populations of aedes aegypti were collected in 1976 and 1979 from a number of different areas of new orleans and analyzed for genetic variation of 12 isozyme loci. although six loci were polymorphic, the majority of loci showed no significant genetic differentiation over time or between regions. the greatest amount of genetic differentiation was found between populations bordering the mississippi river and the two inland areas, mid-city and mount olivet. the low amount of genetic heterogeneity a ...19827102920
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