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structurally related bacillus thuringiensis delta-endotoxins display major differences in insecticidal activity in vivo and in vitro.many strains within the 22 serotypes of bacillus thuringiensis produce crystal delta-endotoxins with slight differences in their insecticidal toxicity spectrum in vivo. since the basis of this specificity is unknown, we chose to compare the activity of delta-endotoxins from three strains: b. thuringiensis var. kurstaki hd-1, var. aizawai hd-249 and var. thuringiensis hd-350, both in vivo and on insect cell lines in vitro. immunoblotting with antisera to activated var. kurstaki p1 lepidopteran to ...19863027111
[biochemical manifestations of the poisoning of larvae of aedes aegypti (insecta, diptera) by the delta-endotoxin of bacillus thuringiensis israelensis. i. hemolymph carbohydrates].the glycemia of 4th instar larvae of aedes aegypti (diptera culicidae) was determined over a 30 min to 36 hrs period following their immersion in water containing 0.00-0.01-0.02-0.1-1.0 mg per liter of a 12,000 i. u. per mg preparation of the delta-endotoxin of bacillus thuringiensis israelensis. seven major carbohydrate fractions were separated in the haemolymph, among which glycogen, trehalose and glucose were identified; the most concentrated of the 4 remaining unknown fractions, exhibits a s ...19863030517
characterization of the genes encoding the haemolytic toxin and the mosquitocidal delta-endotoxin of bacillus thuringiensis israelensis.the crystalline parasporal inclusions (crystals) of bacillus thuringiensis israelensis (bti), which are specifically toxic to mosquito and black fly larvae, contain three main polypeptides of 28 kda, 68 kda and 130 kda. the genes encoding the 28 kda protein and the 130 kda protein have been cloned from a large plasmid of bti. escherichia-coli recombinant clones containing the 130 kda protein gene were highly active against larvae of aedes aegypti and culex pipiens, while b. subtilis recombinant ...19863031426
complement effects on the infectivity of plasmodium gallinaceum to aedes aegypti mosquitoes. i. resistance of zygotes to the alternative pathway of complement.gametocytes are the intraerythrocytic stages of malaria parasites that infect mosquitoes. when gametocytes of the chicken malaria parasite plasmodium gallinaceum are ingested by a mosquito they become extracellular in the mosquito midgut, form gametes, and fertilize within 10 to 15 min after the insect has taken a blood meal. gametocytes of p. gallinaceum were infectious when fed to aedes aegypti mosquitoes in blood meals containing native serum from chickens or from the non-host species, man or ...19863517168
bacteriolytic factor in the salivary glands of aedes aegypti.salivary gland homogenates from adult aedes aegypti lyse micrococcus lysodeikticus cells. the bacteriolytic factor is present in a cell type common to both male and female mosquitoes, as well as in the crop of sugar-feeding mosquitoes. the bacteriolytic factor releases digestion products from sacculi of escherichia coli that are different from those of hen egg white lysozyme.19863519067
aedes aegypti mosquitoes in the americas: a review of their interactions with the human population.this paper describes the significant changes in the relationship between aedes aegypti mosquitoes and the human population in the americas from the 1800s to present. first, the history of the arrival of the mosquito, the consequent epidemics of yellow fever and dengue, and the eventual implication of the mosquito as vector is described. second, the pan american health organization's aedes aegypti eradication program is discussed and the potential threat to the americas of development of the deng ...19863532349
effect of nosema algerae vavra and undeen spores infection on longevity and fecundity of larval instars of aedes aegypti l. and culex fatigans weid. 19863108151
growth of three mosquitoes on two larval diets measured by protein accumulation.larval growth recorded as accumulation of protein was measured in aedes aegypti, culex nigripalpus and culex quinquefasciatus, raised on liver powder or dry brewer's yeast. in the early stages all 3 species grew faster on liver powder, but at pupation there was no significant difference in protein content between diets and among species. aedes aegypti pupated one day ahead of culex.19862906975
comparative studies of metarhizium anisopliae and tolypocladium cylindrosporum as pathogens of mosquito larvae.mosquito fungal pathogens, metarhizium anisopliae and tolypocladium cylindrosporum, were compared with regard to virulence against the larvae of aedes aegypti, anopheles stephensi and culex pipiens. culex pipiens larvae were much more susceptible to m. anisopliae conidia than an. stephensi or ae. aegypti. but ae. aegypti and cx. pipiens larvae were equally susceptible to t. cylindrosporum propagules which weakly attack an. stephensi. using a high concentration conidial suspension (10(7) sp/ml) o ...19862906985
transovarial transmission of tahyna virus by aedes aegypti mosquitoes (strain bangkok) 19862873736
[effect of delta-endotoxin of bacillus thuringiensis israëlensis on biochemical functional relationships in diptera aedes aegypti].a study of the action of very low doses of the delta-endotoxin of bacillus thuringiensis israëlensis on the larvae of aedes aegypti (diptera) gave evidence for peculiar properties of the dose/effect relations based on the variations of the regression slopes of functional relationships between the pairs cysteine/serine and fatty acids/histidine, by contrast with the pair fatty acids/glucose which exhibited a classical shaped relation. this indicates "crypto-toxicological" processes, not lethal by ...19862876759
[dengue vector density survey in liuchiu, pintung, taiwan].the first outbreak of dengue in taiwan in 35 years was recorded in the summer of 1981 in liuchiu, pintung, a small offshore island of southern taiwan. an investigation team visited the affected area during the period of 14-17 october 1981 and collected 8,641 mosquito larvae, of which 4,432 were aedes aegypti and 4,182 were ae. albopictus. the house index, container index and breteau index were 44.2, 23.9 and 73.0 for ae. aegypti and 41.3, 17.0 and 51.9 for ae. albopictus, respectively. both spec ...19862880696
natural transmission of dirofilaria immitis by aedes aegypti.the liverpool strain of the mosquito aedes aegypti was infected with microfilariae of the canine heartworm, dirofilaria immitis, and was used to transmit heartworm larvae to three dogs. methods of confirming heartworm infection in these dogs included the modified knott's test, a commercial enzyme-linked-immunosorbent assay (elisa), an indirect fluorescent antibody (ifa) test, and post-mortem examination.19863507470
evaluation of the cdc gravid trap for the surveillance of st. louis encephalitis vectors in memphis, tennessee.collections with cdc gravid mosquito traps on 954 trap-nights from may through september 1983 are compared to 2,608 concurrent resting site collections made in the same area of memphis, tennessee. gravid traps yielded 88 times more culex per collection and 96 times more culex per man hour. the total gravid trap catch was 135,724 mosquitoes, 99% of which were either culex pipiens or cx. restuans, whereas these species comprised only 63% of the 6,613 mosquitoes collected from resting sites. gravid ...19863507491
effects of sublethal exposure to bacillus thuringiensis var. israelensis on larval development and adult size in aedes aegypti.the effects of exposure to sublethal concentrations of bacillus thuringiensis var. israelensis (serotype h-14) on second instar aedes aegypti larvae were investigated. a test system was developed in which adverse effects would be detected as increased duration of larval development and decreased adult body size. no evidence of negative effects on survivors could be detected when sufficient b.t.i. dosages were applied to kill approximately half of the larvae in the treatment groups. however, when ...19863507506
the perspective of aedes albopictus from the administrative viewpoint.mosquito control administrators should consider a newly introduced species in view of local conditions. aedes albopictus was discovered in harris county while surveying for aedes aegypti. recognizing a potential threat to public health, local and state officials were notified of the discovery. questions posed by the introduction of a foreign species requires a cooperative application of research resources. the centers for disease control (cdc) should serve as the coordinator of this effort.19863507521
toxicity in carcasses of bacillus thuringiensis var. israelensis-killed aedes aegypti larvae against scavenging larvae: implications to bioassay. 19863507532
comparison of three fenitrothion controlled-release formulations as larvicides for aedes aegypti (l.) (diptera: culicidae). 19854078135
the duration of larval life of aedes aegypti as affected by time of hatch.when aedes aegypti eggs (1-10 wk old) were submerged in water, larvae that hatched in the 24 hr period following exposure to reduced oxygen developed significantly faster than larvae from the initial hatch. there were significant differences in the sex ratios between first- and second-hatch groups; however, adult size and fecundity did not differ significantly.19853880263
location of esterase loci in aedes aegypti.linkage relations of two loci, est-4 and est-6, have been studied in aedes aegypti. est-4 is on chromosome i, the sex chromosome, about 33 units from the sex region on the opposite side from red eye (re). this location is demonstrated for the first time. data for est-6 agree with results of other workers.19853880258
egg maturation and ecdysiotropic activity in extracts of mosquito (aedes aegypti) heads.blood-fed, decapitated female aedes aegypti mosquitoes matured eggs when injected with an extract of heads, but non-blood-fed females did not. the response to head extract was optimal when the head was allowed to remain for 2 hr after feeding. a dose response to injected egg development neurosecretory hormone (ednh) was observed in vivo that was similar to in vitro dose responses previously reported. blood-fed decapitated females responded equally well to boiled or unboiled head extract. when bl ...19854065529
the susceptibility of aedes aegypti (l) larvae to a pathogenic non sexual saprolegnia sp. 19853833712
preliminary isolation of mosquito natriuretic factor.a natriuretic factor that triggers diuresis in isolated malpighian tubules of the mosquito was isolated from the head of the yellow-fever mosquito aedes aegypti by passing a saline extract of mosquito heads through low-pressure and then high-pressure liquid chromatography (hplc) columns. three fractions with biologic activity eluted during a reverse-phase hplc linear acetonitrile gradient run. fraction i depolarized the transepithelial voltage (vt) of isolated perfused malpighian tubules but did ...19852413779
high-resolution gradient polyacrylamide electrophoresis of isozymes of the mosquito, aedes aegypti.a procedure for high resolution electrophoresis of isozymes of the mosquito aedes aegypti using nonlinear (2.5%-20%) polyacrylamide gradient gel slabs is described. crude mosquito homogenates were electrophoresed on gradient and on homogeneous (7%) polyacrylamide gels and stained for esterase (est), isocitrate dehydrogenase (idh) and malate dehydrogenase (mdh). the resulting zymograms were compared to demonstrate the high resolving power of the polyacrylamide gradient gel system.19853880252
ethyl-methanesulfonate-induced changes in filarial susceptibility in aedes aegypti (diptera: culicidae). 19854045933
efficacy of three insect growth regulators on the development of aedes aegypti. 19853880238
wind tunnel evaluation of commonly used adulticides against new orleans aedes aegypti. 19853880235
effects of cyromazin and methoprene on the developmental stages of anopheles dirus, aedes aegypti and culex quinquefasciatus (diptera : culicidae).the effects of two chemical compounds, cyromazin and methoprene, on the developmental stages of anopheles dirus, aedes aegypti and culex quinquefasciatus were investigated under laboratory conditions, with the mean temperature of 24 degrees +/- 1 degree c and the relative humidity at 65-75%. both compounds were tested against the second, third and fourth instar larvae. the concentrations of cyromazin used for an. dirus and cx. quinquefasciatus ranged from 0.0008 to 0.5 mg/l; and for ae. aegypti ...19852866585
effect of weekly releases of toxorhynchites amboinensis (doleschall) on aedes aegypti (l.) (diptera: culicidae) in new orleans, louisiana. 19852861216
response of domestic and peridomestic strains of aedes aegypti (diptera: culicidae) in new orleans, louisiana, usa, to organophosphate, organochlorine, and pyrethroid insecticides. 19854009625
dominant lethal effects of thiotepa in male aedes aegypti (diptera: culicidae). 19853925149
hormone-mediated formation of the endocytic complex in mosquito oocytes.the developmental events leading to oocyte competence to internalize proteins, and the hormonal control of these events in the mosquito aedes aegypti have been studied. the oocytes of newly eclosed females have an undifferentiated cortex. during previtellogenic development, a highly specialized endocytic complex, consisting of numerous coated vesicles and uncoated endosomes, microvilli, and, presumably, vitellogenin receptors, forms in the oocyte cortex. morphometric analysis and probes with a p ...19853988025
mathematical models of the effectiveness and persistence of mosquito repellents.two models of the effectiveness and persistence of mosquito repellents on the skin were developed from published functions and data. the probit plane model, y = a + b1x1 + b2x2, relates the response (y, in probits) of the mosquito test population to the log dose (x1) of repellent applied and the test period, or elapsed time from the time of application (x2). the exponential decay model, z = x1 + (b2/b1)x2, estimates the repellent residue (log-1 z) from x1 and x2. the models were validated with o ...19852906657
use of an artificial bromeliad to show the importance of color value in restricting colonization of bromeliads by aedes aegypti and culex quinquefasciatus.an artificial bromeliad was developed which, painted and containing an infusion water, elicited ovipositional response by caged adult aedes aegypti, culex quinquefasciatus, wyeomyia vanduzeei and wy. mitchellii. comparison was made of the ovipositional response of adults of the four mosquito species to artificial bromeliads painted black, white, dark green and deep blue. adult ae, aegypti and wy. vanduzeei did not discriminate significantly between white, dark green and deep blue, but whereas ae ...19852906654
effect of selection for dichlorodiphenyltrichloroethane (ddt) resistance on the uptake and breakdown of ddt in aedes aegypti l.aedes aegypti larvae and adults were selected to high levels of resistance with dichlorodiphenyltrichloroethane (ddt) along separate lines. the larval-selected line showed three responses associated with larval resistance: increased detoxication of ddt by dehydrochlorination to dichlorodiphenyldichloroethane dde (demonstrated both in vivo and in vitro), increased tolerance to unmetabolised ("residual") ddt and, a reduction in uptake of ddt. larval selection caused very little change in adult res ...19853986671
reduced productivity in tretamine-treated adult populations of aedes aegypti (diptera: culicidae). 19853981551
ultrastructure of midgut endocrine cells in the adult mosquito, aedes aegypti.the ultrastructure of endocrine cells in the midgut of the adult mosquito, aedes aegypti, resembled that of endocrine cells in the vertebrate gastro-intestinal tract. midgut endocrine cells, positioned basally in the epithelium as single cells, were cone-shaped and smaller than the columnar digestive cells. the most distinctive characteristic of endocrine cells was numerous round secretory granules along the lateral and basal plasma membranes where contents of the granules were released by exocy ...19854060146
transition from somatic to meiotic pairing and progressional changes of the synaptonemal complex in spermatocytes of aedes aegypti.aedes aegypti spermatocytes were reconstructed from electron micrographs. the species has tight somatic pairing of the chromosomes, and there are therefore no classical leptotene and zygotene stages, but rather a gradual transition from somatic pairing to meiotic pairing (= pachytene). the term "prepachytene" has been used for the transitory stage. the first visible sign of impending meiosis was a reorganization of the chromatin, which resulted in the formation of spaces (synaptic spaces) in the ...19854042770
[biology and behavior of aedes aegypti]. 19853843130
tests of ultrasonic emissions on mosquito attraction to hosts in a flight chamber.five ultrasonic devices generating fundamental frequencies of 20-70 khz were tested for their efficacy in repelling mosquitoes. four species (anopheles quadrimaculatus, aedes aegypti, ae. triseriatus and haemagogus equinus), were used in a flight chamber in which females must fly upwind against the direction of the sound waves and around the ultrasonic devices to reach a trap downwind of a source of human breath and skin emanations. repellency was rated by the number of mosquitoes entering the t ...19852906666
molecular cloning of the delta-endotoxin gene of bacillus thuringiensis var. israelensis.a transformant of bacillus megaterium, vb131, was isolated which carries a 6.3-kb xbai segment of the crystal toxin gene of bacillus thuringiensis var. israelensis (bti) cloned in a vector plasmid pbc16 to yield pvb131. the chimeric plasmid dna from vb131 was introduced into a transformable bacillus subtilis strain by competence transformation. both the b. megaterium vb131 strain and the b. subtilis strain harboring the chimeric plasmid produced irregular, parasporal, phase-refractile, crystalli ...19852987085
baculovirus-mediated expression of bacterial genes in dipteran and mammalian cells.a recombinant baculovirus containing the escherichia coli chloramphenicol acetyltransferase (cat) gene under the control of the rous sarcoma virus long terminal repeat promoter and the e. coli beta-galactosidase gene under the control of the very late baculoviral polyhedrin promoter was used to determine if autographa californica nuclear polyhedrosis virus, a baculovirus of lepidoptera, can enter and express viral dna in dipteran (drosophila sp.) and mammalian (mus sp.) cells that are considered ...19852993646
micro-lipid-droplet encapsulation of bacillus thuringiensis subsp. israelensis delta-endotoxin for control of mosquito larvae.the crystal delta-endotoxin of bacillus thuringiensis subsp. israelensis is less toxic to larvae of anopheles freeborni than to larvae of aedes aegypti. however, when solubilized crystal was used, larvae from both species showed similar sensitivities. this effect presumably was due to the differences in feeding behavior between the two mosquito larvae when crystal preparations are used. a procedure is described whereby both crystal and solubilized b. thuringiensis subsp. israelensis toxin were e ...19853002272
comparative susceptibility of mosquito species and strains to oral and parenteral infection with dengue and japanese encephalitis viruses.thirty-four strains of asian and pacific mosquitoes belonging to 22 species of 7 genera were compared for oral and/or parenteral susceptibility to infection with 1 or more strains of each of the 4 dengue serotypes. surprisingly, several species of common man-biting aedes were much more susceptible to oral infection with each of the 4 dengue serotypes than was aedes aegypti. these species included aedes albopictus and members of the scutellaris group of the subgenus stegomyia found on south pacif ...19852860816
disease-bearing insects brought in by international aircraft into singapore.fifty-seven (17.3%) of 330 incoming aircraft landing at changi international airport were found to harbour mosquitoes and other insects on board. the frequency of insects detected in aircraft from the southeast asian countries varied from 7.1% to 32.2%. live insects were found in 5 (6.1%) of 82 aircraft disinsectized by the "block away" method. the species of mosquitoes brought in were: culex quinquefasciatus (96), culex tritaeniorhynchus (1), aedes albopictus (2) and aedes aegypti (1). none of ...19852862709
arboviruses and lemurs in madagascar: experimental infection of lemur fulvus with yellow fever and west nile viruses.in previous serological surveys of lemurs in madagascar, antibodies against flaviviruses were frequently detected. to examine the epidemiological role of lemur fulvus, experimental infections with yellow fever (yf) virus and west nile (wn) virus were performed. yf and wn infections were clinically unapparent. a 3 to 4-day-long viremia, with moderate levels was observed with yf virus. wn virus, especially the strain isolated in madagascar, provoked a 4 to 6-day-long viremia sufficient to infect a ...19852862804
experimental study of the reproduction of karshi virus (togaviridae, flavivirus) in some species of mosquitoes and ticks.the strain kaz-816 of karshi virus was isolated in 1976 from h. asiaticum ticks collected in the north of central asia (alma-ata region of the kazakh soviet socialist republic). both ticks and mosquitoes can be vectors of karshi virus as proved experimentally by reproduction of the virus in hyalomma asiaticum and dermacentor daghestanicus ticks and culex pipiens molestus, anopheles atroparvus an aedes aegypti mosquitoes as well as by transmission to newborn mice by the bite of infected mosquitoe ...19852864824
variable infectivity of third-stage larvae of brugia malayi.infectivity of third-stage larvae of brugia malayi was assessed following intraperitoneal inoculation into jirds, meriones unguiculatus. larvae were of two ages and were derived from two sites in aedes aegypti mosquitoes, i.e., specimens collected from the thorax 11 days after infection and from the head on day 14. larvae from the thorax had just completed the second moult and measured 990 to 1100 micron in length. only 6% of these specimens developed to adult worms in jirds. larvae that migrate ...19852866213
variations of four aedes aegypti mosquito strains in their susceptibility to and transmissibility of tahyna virus.aedes aegypti mosquitoes originating from different colonies were infected with tahyna virus by sucking on viraemic newborn mice (peak titre 10(2) x 5 mouse i.c. ld50/0.01 ml). the mosquitoes of laboratory strain "london" proved most susceptible (infection rate 74.2%, transmission rate 60%), while mosquitoes of the laboratory strain "basel" and strain "bangkok" (southeast asia) were less susceptible (infection rate 23.7 and 17.6%, respectively, transmission rate 6.0 and 10.0%, respectively). mos ...19852866696
[abundance of larvae of urban mosquitoes during the campaign of the eradication of aedes aegypti (linnaeus, 1762) and of dengue in cuba (1981-82)]. 19852870540
avermectins, mk-933 and mk-936, for mosquito control.two avermectins were shown to be effective as mosquito larvicides or when fed to adult female mosquitoes in sucrose solution or in blood. larval lc50 values of compounds mk-933 and mk-936, expressed as parts per billion, were found to be 3.94 and 2.42 for culex pipiens, 5.85 and 2.90 for anopheles stephensi and 23.4 and 10.4 for aedes aegypti. when fed to adult females of an. stephensi, ae. aegypti or cx quinquefasciatus, mk-933 at 2.8mg active ingredient per litre in sucrose solution caused com ...19853832491
oral infection of aedes aegypti with yellow fever virus: geographic variation and genetic considerations.twenty-eight populations representing a worldwide distribution of aedes aegypti were tested for their ability to become orally infected with yellow fever virus (yfv). populations had been analyzed for genetic variations at 11 isozyme loci and assigned to one of 8 genetic geographic groups of ae. aegypti. infection rates suggest that populations showing isozyme genetic relatedness also demonstrate similarity to oral infection rates with yfv. the findings support the hypothesis that genetic variat ...19853834804
selection for susceptibility and refractoriness of aedes aegypti to oral infection with yellow fever virus.artificial selection on strains of aedes aegypti showing susceptibility and refractoriness to oral infection with yellow fever virus (yfv) suggests that there is a significant genetic component to this trait. using a population with an average susceptibility of 15%, inbreeding of isofemale lines followed by individual selection produced susceptible (29% infected) and refractory (11% infected) lines. the difference between lines was largely apparent before individual selection, which failed to in ...19853834805
rainfall, abundance of aedes aegypti and dengue infection in selangor, malaysia.an epidemio-meteorotropic analytical study of selangor, in the southwest coast of peninsular malaysia, examines the monthly incidence of dengue for the period 1973-1982 to assess possible quantitative association with the monthly rainfall. the relationships between rainfall, abundance of a. aegypti and dengue infection during 1982 in jinjang, a dengue-prone area in selangor, were also examined. a quantitative association between rainfall and the number of dengue cases was found during the first ...19853835698
[new data on the transmission of filariae].this paper summarizes research of the past several years on two phases of filarial transmission: the ingestion of microfilariae by the vector and the regulation of the percentage of successful parasites by the vector. experiments involved several different models for study: injection of inert particles into the bloodstream of a rodent and their subsequent ingestion by a vector; ingestion of gametocytes of different ages by vectors of a rodent malaria; and a monkey simultaneously infected with fo ...19853836764
aedes aegypti: model for blood finding strategy and prediction of parasite manipulation.aedes aegypti mosquitoes salivate during intradermal probing of vertebrate prey before ingesting blood (griffiths and gordon 1952). nonsalivating mosquitoes locate blood more slowly; this difference was ascribed to an anti-platelet activity found in the mosquito's saliva (ribeiro et al. 1984). mosquitoes infected with plasmodium gallinaceum suffer pathology that specifically impairs saliva anti-hemostatic activity but without reducing volume of output (rossignol et al. 1984). the complexity of t ...19853839460
[present viability of the eradication of aedes aegypti in the control of yellow fever in brazil]. 19853843131
geographic genetic differentiation and arbovirus competency: aedes aegypti and yellow fever. 19853870635
a simple method of identifying organophosphate insecticide resistance in adults of the yellow fever mosquito, aedes aegypti.a simple filter paper spot test is described for identifying adult aedes aegypti resistant to malathion as a result of raised esterase levels. the method is compared with established polyacrylamide gel (pag) electrophoresis techniques for determining esterase-6 activity, and its applicability as a field test is discussed.19853880211
laboratory evaluation of controlled-release insect repellent formulations.seven microcapsule formulations and two polymer formulations of deet were tested on white rabbits for their repellency against the mosquito, aedes aegypti. two microcapsule formulations and one polymer formulation provided more than 80% protection for 12 hours. results demonstrated that the protection period of deet can be extended through controlled-release techniques.19853880224
development of dirofilaria immitis third stage larvae (nematoda: filarioidea) in micropore chambers implanted into surrogate hosts.groups of 100 third stage larvae of dirofilaria immitis recovered from aedes aegypti were loaded into 250 microliters capacity micropore chambers (0.3 micron pore size) and implanted into the peritoneal cavity of mice, jirds, cotton rats and ferrets. in all hosts 74-87% of larvae moulted by 74 hours, with less than 5% mortality. the fourth stage worms recovered at 74 hours were cultured in vitro in l-15 (leibovitz) medium plus 20% foetal bovine serum with a dog sarcoma feeder cell line. after 96 ...19853890119
viability of mycobacterium leprae within the gut of aedes aegypti after they feed on multibacillary lepromatous patients: a study by fluorescent and electron microscopes. 19853908862
development of mansonella ozzardi in the liverpool strain of aedes aegypti. 19853914123
mechanical transmission of rift valley fever virus by hematophagous diptera.experimental studies were conducted to determine if hematophagous diptera were capable of mechanical transmission of rift valley fever (rvf) virus to laboratory animals. all species tested (glossina morsitans, aedes aegypti, aedes taeniorhynchus, culex pipiens, stomoxys calcitrans, lutzomyia longipalpis, and culicoides variipennis) mechanically transmitted the virus to hamsters. mechanical transmission rates for g. morsitans ranged from 0-100%, with the probability of mechanical transmission pos ...19853970308
plasmodium gallinaceum: sporozoite activity in immune mosquito hemolymph.sporozoites of plasmodium gallinaceum are relatively inactive in hanks' balanced salt solution or in the hemolymph of the susceptible aedes aegypti mosquito. they become agitated and very active in the presence of the hemolymph of the innately immune mosquito, culex pipiens. sporozoites from the latter are more infective in chicks than the former. this behavior is likely the result of stimulation or irritation by the adverse environment.19853972059
characterization of refractoriness in aedes aegypti (diptera: culicidae) to infection by dirofilaria immitis. 19853981554
[sensitivity of aedes aegypti infected with the malaria agent to ddt]. 19853990637
the effect of different types of skin surfaces on the transmission of brugia pahangi infective larvae by the mosquito aedes aegypti.the transmission of brugia pahangi from aedes aegypti into meriones unguiculatus was compared for four different skin surfaces (hairy, cloth, clean, greasy). unshaven jirds reduced the feeding avidity of infective mosquitoes. the loss of larvae from infective mosquitoes was greatest when these insects fed on "exposed" skin surfaces. significantly fewer infective larvae penetrated the host when infective mosquitoes fed on a jird through a thin layer of cloth.19853992643
failure to detect natural transovarial transmission of dengue viruses by aedes aegypti and aedes albopictus (diptera: culicidae). 19854009622
bioassays of bacillus sphaericus (strain 1593) against mosquitoes of public health importance in malaysia.the pathogenicity of bacillus sphaericus strain 1593 was tested against laboratory-reared larvae of four local species of mosquitoes of public health importance in malaysia; aedes aegypti, anopheles balabacensis, mansonia uniformis and culex quinquefasciatus. the bacteria was shake-cultured at 28 +/- 1 degrees c for three days, using glucose-yeast extract salts medium. after which, the spores and vegetative cells were harvested and stored at 4 degrees c before use. conditions for bioassays were ...19854023816
effect of dirofilaria immitis on blood meal size and fecundity in aedes aegypti (diptera: culicidae). 19854045936
[effect of mosquito contact with ddt and their susceptibility to the causative agent of malaria].the contact of aedes aegypti mosquitoes with ddt one hour prior to the infectious feeding results in a slight decrease of their susceptibility to plasmodium gallinaceum. the contact 24 hours prior to the infectious feeding does not affect the susceptibility of mosquitoes.19854047719
dengue and hepatic failure.dengue hemorrhagic fever/dengue shock syndrome is a serious manifestation of dengue fever, which is observed predominantly in the tropical regions of the west pacific and in southeast asia and is associated with secondary infections, mainly in children under age 15. a concomitant microangiopathic coagulopathy has been described; moreover, encephalopathy and even reye's syndrome have been rarely reported. this report describes a 51-year-old man with secondary dengue infection who presented with c ...19854061482
dibutyryl-camp increases basolateral sodium conductance of mosquito malpighian tubules.dibutyryladenosine 3',5'-cyclic monophosphate (camp) stimulates fluid secretion in isolated malpighian tubules of the mosquito aedes aegypti. in the present study the effects of camp on the basolateral membrane were studied with conventional microelectrodes. membrane conductances were evaluated from the changes of the basolateral membrane potential (vbl) consequent to ion changes in the bath. under control conditions, vbl measured -65.2 +/- 1.5 mv [83 impalements, 67 tubules]. a fivefold decreas ...19852579589
evaluation of slow-release formulations of temephos (abate) and bacillus thuringiensis var. israelensis for the control of aedes aegypti in puerto rico.formulations of temephos (abate) and bacillus thuringiensis var. israelensis (b.t.i.) on corncob and dried coconut husk carriers were tested for slow-release insecticide properties against aedes aegypti larvae in puerto rico. granular formulations of 5% and 10% temephos gave continuous larval control in used automobile tires for 27 to 124 days and 34 to 162 days, respectively. temephos on radial sections of corncob and coconut husk chips gave good larval control in tires for 27 to 63 and 61 to 1 ...19852466106
the inactivation of acetylcholinesterase by alpha-terthienyl and ultraviolet light. studies in vitro and in larvae of the mosquito aedes aegypti.acetylcholinesterase (ec 3.1.1.7) was inactivated photochemically in solution, in the presence of dissolved terthiophene sensitizers. alpha-terthienyl (2,2':5,2"-terthiophene) and its isomers 3,2':5',2"- and 3,2':5',3"-terthiophenes showed very similar sensitizing properties. with all three terthiophenes, the photosensitization was completely suppressed under anaerobic conditions, and therefore the inactivation process required the presence of oxygen. the enzyme was inactivated in vivo when four ...19846509086
genetic variation in an urban population of aedes aegypti (diptera: culicidae). 19846502629
amino acids and peptides. xi. synthesis of attractant and repellent peptides for aedes aegypti and blattella germanica. 19846532560
paradoxical effects of simulated larviciding on production of adult mosquitoes.we determined whether simulated mortality of larval mosquitoes in food-deprived bodies of water increases emergence of adult females as well as their size by releasing the remaining larvae from competition for food. removal of larval aedes aegypti from rearing containers with suboptimal amounts of food increased the number of adults that emerged. thus, larvicidal measures do not inevitably lead to reduced numbers of mosquito vectors of disease. in food-limiting breeding sites, partially effectiv ...19846507734
quantitative structure-activity relationship of insect juvenile hormone mimetic compounds.juvenile hormone mimetic activities on aedes aegypti (yellow-fever mosquito) and tenebrio molitor (yellow mealworm) of compounds having (2e,4e)-3,7,11-trimethyl-2,4-dodecadienone structures were comparatively and quantitatively analyzed in terms of their physiochemical structural parameters and by regression analysis. they were structurally composed of three classes, ester and thiol ester derivatives, amides, and ketones, depending on the c1 substituents. the results indicated that the steric di ...19846492079
hormonal regulation of ovarian ecdysteroid production in the autogenous mosquito, aedes atropalpus.the effects of juvenile hormone (jh) and egg development neurosecretory hormone (ednh) on ovarian ecdysteroid production during vitellogenesis in the autogenous mosquito, aedes atropalpus, were investigated using in vitro techniques coupled with radioimmunoassay (ria) and high-pressure liquid chromatography (hplc). normal females were characterized by quantitative, qualitative, and temporal patterns of in vitro ovarian ecdysteroid production. females decapitated at emergence showed little ovaria ...19846541607
esterase activity in strains of aedes aegypti (diptera: culicidae) tolerant and susceptible to the organophosphate insecticide malathion. 19846492084
lithium attenuates the active transport of calcium in the larva of aëdes aegypti. 19846491591
the toxic effects of triorganotin(iv) compounds on the culicine mosquito, aedes aegypti (l).the structure-activity relationships of 25 triorganotin(iv) compounds were studied using the 4th instar a. aegypti larvae of the susceptible liverpool red-eye strain. the lc50 values of these compounds were compared with those of malathion, ddt and temephos. maximal activities were obtained with tritolyltin chloride and its complexes with triphenyl-phosphine oxide and pyridine n-oxide, dimethyloctyltin- and diethyloctyltin acetates and tributyltin sucrose phthalate. a limited number of the trior ...19846204424
effects of toxorhynchites amboinensis on laboratory-reared aedes aegypti populations (diptera: culicidae). 19846144797
salivary apyrase of aedes aegypti: characterization and secretory fate.salivary gland homogenates of female adult aedes aegypti hydrolyzed atp and adp thereby defining an apyrase activity. activity is divalent cation dependent with an optimum ph of 9.0. atpase and adpase activities could not be dissociated thus suggesting the presence of a true apyrase enzyme. apyrase activity is low on the day of emergence but increases to 160 mu per pair of glands on the second day. the site at which mosquitoes probed into warm polyacrylamide gels retains apyrase activity, confir ...19846094095
specificity of cultured insect tissue cells for bioassay of entomocidal protein from bacillus thuringiensis.cultured tissue cells from lepidopteran and dipteran sources displayed an order-specific response to entomocidal protein from crystals of bacillus thuringiensis. protein isolated from crystals of b. thuringiensis subsp. kurstaki was effective against cells of the spruce budworm (choristoneura fumiferana) and the tobacco hornworm (manduca sexta), but was inactive against both mosquito cell lines tested (aedes aegypti and anopheles gambiae). conversely, protein from inclusion bodies of b. thuringi ...19846321329
a survey of filariasis among refugees in south florida.between january 1981 and march 1982, a filariasis survey was conducted among 668 haitian immigrants and 155 southeast asian refugees residing in florida, u.s.a. microfilariae were detected only in haitians, with 6.7% positive for wuchereria bancrofti and 1.3% positive for mansonella ozzardi. the majority of individuals with bancroftian filariasis came from five coastal urban areas including port-au-prince, duvalierville, gonaïves, leógane and cap-haitien. no unequivocal signs of bancroftian fila ...19846370004
[ecology of dengue and aedes aegypti. preliminary study]. 19846372125
selective primary health care: strategies for control of disease in the developing world. xi. dengue.since world war ii, dengue viruses have progressively extended their geographic domain and have increased as causes of human morbidity and mortality. this complex of four flaviviruses is principally transmitted to humans by the bite of aedes aegypti, the yellow fever vector. factors that promote the indoor storage of water are congenial to the breeding of a. aegypti. these include the dislocations of wars, overpopulation , and urbanization. by the mid-20th century, a. aegypti eradication campaig ...19846374837
arthropod studies with rabies-related mokola virus.a cell culture-adapted variant of the rabies-related mokola virus was demonstrated to replicate in inoculated aedes aegypti mosquitoes. replication was slow compared to many arboviruses in their vectors. maximum titers were not obtained until after approximately 6 weeks of extrinsic incubation. mokola virus underwent nine mosquito-mosquito passages at approximately monthly intervals and was thus maintained in insects for 340 days before terminating the study. virus antigen was detected by immuno ...19846385743
[the initial activity and persistance of an experimental larvicide with a base of bacillus thuringiensis var. israelensis and a commercial preparation containing the organophosphorus insecticide temephos].the larvicide activity of the experimental preparation san 402 i wdc containing b. thuringiensis var. israelensis and that of abate 500 e (44 % temephos) were tested on the larvae of aedes aegypti. the studies were performed on the following media: distilled water, pure and buffered at ph 9, 7 and 4 and pond water whether or not free from materials in suspension. the activity of both preparations is not influenced by the composition of the media, exception made for the presence of materials in s ...19846208235
[the ecology of dengue and aedes aegypti. preliminary investigation 4]. 19846084309
the importance of disease induced changes in mammalian body temperature to mosquito blood feeding.laboratory mice infected with rodent malaria (plasmodium berghei or p. chabaudi) or st. louis encephalitis virus (sle) were not hyperthermic during the infection period. however, all infected animals displayed pathogen-specific periods of hypothermia. hamsters infected with p. berghei were hyperthermic on day 7 postinfection (pi) but became hypothermic on day 8 pi and remained so until death, approximately 20 days pi. body temperatures of mice infected with p. yoelii were not significantly diffe ...19846142803
the effect of p-aminobenzoic acid and folic acid on the development of infective larvae of brugia malayi in aedes aegypti.adult aedes aegypti mosquitoes, infected with the subperiodic brugia malayi, were found to enhance the development of the filarial parasites to the infective stage when they were exposed to a cotton pad soaked in 10% sucrose solution containing p-aminobenzoic acid (paba) in 0.001, 0.005, 0.01, 0.05 and 0.1% concentrations. similarly, larval development increased when the mosquitoes were fed with folic acid at 0.001, 0.01 and 0.1% concentrations. this stimulation was more when paba or folic acid ...19846143484
medium for the production of primary powder of bacillus thuringiensis subsp. israelensis.five media, formulated from the seeds of five legume varieties, dried cow blood, and mineral salts, were assessed for the growth and production of insecticidal properties of bacillus thuringiensis subsp. israelensis. bacterial powders prepared from the broth cultures were assayed against the larvae of aedes aegypti, culex quinquefasciatus, and anopheles gambiae. a standard primary powder of b. thuringiensis subsp. israelensis (ips78) was included in the assay for comparison. good growth was obta ...19846144290
[preliminary report on the susceptibility of mosquitoes to brugia pahangi].a comparative study on the susceptibility of eight species of four genera of mosquitoes to brugia pahangi was carried out. aedes aegypti (liverpool strain), aedes togoi, armigeres subalbatus and mansonia uniformis (taiwan strains) were found with the third-stage larvae 14 days after feeding on infected rats (sprague-dawley). the remaining of aedes aegypti, aedes albopictus, aedes pseudoalbopictus, culex p. fatigans and culex annutus (taiwan strains) were found refractory to infection. to determi ...19846150813
comparative bioassays of tolypocladium cylindrosporum gams (californian strain) against four species of mosquitoes in malaysia.comparative laboratory bioassays of tolypocladium cylindrosporum, california strain (kal) was conducted against third instar larvae of four species of mosquito, viz. aedes aegypti, anopheles balabacensis, culex quinquefasciatus and mansonia uniformis in malaysia. of the four mosquito species tested, ma. uniformis was found to be the most susceptible, followed by cx. quinquefasciatus, an. balabacensis and ae. aegypti, in a decreasing order. the lc50 values for ma. uniformis, cx. quinquefasciatus, ...19846151744
laboratory observations on toxorhynchites splendens wiedemann in thailand.the biology of toxorhynchites splendens predaceous larvae which breed in domestic containers like aedes aegypti was studied. egg stage lasts 52.64 +/- 0.13 hours. there are four larval instars lasting 2.38 +/- 0.04, 1.24 +/- 0.26, 8.09 +/- 1.6 and 28.78 +/- 5.39 days respectively. pupal development lasts 5.18 +/- 1.06 days. the adult life span is 29.55 +/- 8.06 days. growth increment was by measuring the size of head capsule. number of second instar prey larvae consumed by 1 to 4 instars predace ...19846151745
[research on viral infections in south pacific mosquitoes under natural conditions].from 1975, epidemiological studies on arboviral diseases, conducted in new caledonia, vanuatu, wallis and horne islands, have shown that 11 species of mosquitoes may harbour viruses in natural conditions. virus isolations were attempted from 1,025 pools of culicidae totalizing 32,182 individuals belonging to 5 genera and 21 species. viral infections have been demonstrated in 76 pools (7.4%). the species harbouring viruses were: aedes aegypti, ae. alternans, ae. notoscriptus, ae. vexans, ae. vigi ...19846151879
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