Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| correction: the ph-responsive pacc transcription factor of aspergillus fumigatus governs epithelial entry and tissue invasion during pulmonary aspergillosis. | [this corrects the article doi: 10.1371/journal.ppat.1004413.]. | 2015 | 25853727 |
| genome sequence comparison of aspergillus fumigatus strains isolated from patients with pulmonary aspergilloma and chronic necrotizing pulmonary aspergillosis. | aspergillus fumigatus is the aspergillus species most commonly associated with aspergillosis. of the various presentations of aspergillosis, one of the most frequently observed in cases involving a. fumigatus pulmonary infections is aspergilloma (pa). in such infections one finds a fungus ball composed of fungal hyphae, inflammatory cells, fibrin, mucus, and tissue debris. chronic necrotizing pulmonary aspergillosis (cnpa), also known as semi-invasive or invasive aspergillosis, is locally invasi ... | 2015 | 25851262 |
| structure of n-myristoyltransferase from aspergillus fumigatus. | n-myristoyltransferase (nmt) is an enzyme which translocates the 14-carbon saturated fatty acid myristate from myristoyl-coa to the n-terminal glycine of substrate peptides. this myristoylation process is involved in protein modification in various eukaryotes, including animals and fungi. furthermore, this enzyme has been shown to be essential to the growth of various species, such as saccharomyces cerevisiae, which indicates that nmt is an attractive target for the development of a novel antifu ... | 2015 | 25849386 |
| antifungal properties of silver nanoparticles against indoor mould growth. | the presence of moulds in indoor environments causes serious diseases and acute or chronic toxicological syndromes. in order to inhibit or prevent the growth of microorganisms on building materials, the disruption of their vital processes or the reduction of reproduction is required. the development of novel techniques that impair the growth of microorganisms on building materials is usually based on silver nanoparticles (agnps). it makes them an alternative to other biocides. agnps have proven ... | 2015 | 25847174 |
| effects of iron chelators on the formation and development of aspergillus fumigatus biofilm. | iron acquisition is crucial for the growth of aspergillus fumigatus. a. fumigatus biofilm formation occurs in vitro and in vivo and is associated with physiological changes. in this study, we assessed the effects of fe chelators on biofilm formation and development. deferiprone (dfp), deferasirox (dfs), and deferoxamine (dfm) were tested for mic against a reference isolate via a broth macrodilution method. the metabolic effects (assessed by xtt [2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2h-tetraz ... | 2015 | 26239975 |
| identification of the antiphagocytic trypacidin gene cluster in the human-pathogenic fungus aspergillus fumigatus. | the opportunistic human pathogen aspergillus fumigatus produces numerous different natural products. the genetic basis for the biosynthesis of a number of known metabolites has remained unknown. the gene cluster encoding for the biosynthesis of the conidia-bound metabolite trypacidin is of particular interest because of its antiprotozoal activity and possible role in the infection process. here, we show that the genes encoding the biosynthesis enzymes of trypacidin reside within an orphan gene c ... | 2015 | 26278536 |
| transcriptome profiles of human lung epithelial cells a549 interacting with aspergillus fumigatus by rna-seq. | lung epithelial cells constitute the first defense line of host against the inhaled aspergillus fumigatus; however, the transcriptional response of human alveolar type ii epithelial cells was still unclear. here we used rna-seq technology to assess the transcriptome profiles of a549 cells following direct interaction with conidia of a. fumigatus. the total number of identified genes was 19118. compared with uninfected a549 cells, 459 genes were differentially expressed in cells co-incubated with ... | 2015 | 26273834 |
| targeting zinc homeostasis to combat aspergillus fumigatus infections. | aspergillus fumigatus is able to invade and grow in the lungs of immunosuppressed individuals and causes invasive pulmonary aspergillosis. the concentration of free zinc in living tissues is much lower than that required for optimal fungal growth in vitro because most of it is tightly bound to proteins. to obtain efficiently zinc from a living host a. fumigatus uses the zinc transporters zrfa, zrfb, and zrfc. the zafa transcriptional regulator induces the expression of all these transporters and ... | 2015 | 25774155 |
| the malaria co-infection challenge: an investigation into the antimicrobial activity of selected guinean medicinal plants. | in sub-saharan africa, concomitant occurrence of malaria and invasive infections with micro-organisms such as gram-positive staphylococcus aureus, gram-negative escherichia coli and yeasts or fungi such as candida albicans and aspergillus fumigatus is common. non-tuberculous mycobacteriosis caused by mycobacterium chelonae has been recognized as a pulmonary pathogen with increasing frequency without effective therapy. although less important, the high incidence of trichophyton rubrum infections ... | 2015 | 25773488 |
| pathogenic fungi regulate immunity by inducing neutrophilic myeloid-derived suppressor cells. | despite continuous contact with fungi, immunocompetent individuals rarely develop pro-inflammatory antifungal immune responses. the underlying tolerogenic mechanisms are incompletely understood. using both mouse models and human patients, we show that infection with the human pathogenic fungi aspergillus fumigatus and candida albicans induces a distinct subset of neutrophilic myeloid-derived suppressor cells (mdscs), which functionally suppress t and nk cell responses. mechanistically, pathogeni ... | 2015 | 25771792 |
| exploration of aspergillus fumigatus ras pathways for novel antifungal drug targets. | ras pathway signaling is a critical virulence determinant for pathogenic fungi. localization of ras to the plasma membrane (pm) is required for ras network interactions supporting fungal growth and virulence. for example, loss of aspergillus fumigatus rasa signaling at the pm via inhibition of palmitoylation leads to decreased growth, altered hyphal morphogenesis, decreased cell wall integrity and loss of virulence. in order to be properly localized and activated, ras proteins must transit a ser ... | 2015 | 25767465 |
| resistance is not futile: gliotoxin biosynthesis, functionality and utility. | gliotoxin biosynthesis is encoded by the gli gene cluster in aspergillus fumigatus. the biosynthesis of gliotoxin is influenced by a suite of transcriptionally-active regulatory proteins and a bis-thiomethyltransferase. a self-protection system against gliotoxin is present in a. fumigatus. several additional metabolites are also produced via the gliotoxin biosynthetic pathway. moreover, the biosynthesis of unrelated natural products appears to be influenced either by gliotoxin or by the activity ... | 2015 | 25766143 |
| identification of aspergillus fumigatus surface components that mediate interaction of conidia and hyphae with human platelets. | platelets were recently identified as a part of innate immunity. they are activated by contact with aspergillus fumigatus; putative consequences include antifungal defense but also thrombosis, excessive inflammation, and thrombocytopenia. we aimed to identify those fungal surface structures that mediate interaction with platelets. | 2015 | 25810442 |
| highly cadmium tolerant fungi: their tolerance and removal potential. | soil and effluent of lead and zinc industries contain high concentration of cadmium. the present study was conducted to isolate tolerant fungal strains from cadmium -polluted sites in zanjan province, iran. | 2015 | 25806110 |
| fungal isolates from a pu-erh type tea fermentation and their ability to convert tea polyphenols to theabrownins. | the natural microbiota involved in the fermentation influence the quality and taste of fully postfermented teas such as china's pu-erh tea. ten microbial isolates representing 6 species were recovered from a solid-state fermentation of a pu-erh type tea. the isolates were aspergillus tubingensis, aspergillus marvanovae, rhizomucor pusillus, rhizomucor tauricus, aspergillus fumigatus, and candida mogii. with the exception of a. marvanovae and c. mogii, all these microorganisms have been previousl ... | 2015 | 25799937 |
| nebulised amphotericin b-polymethacrylic acid nanoparticle prophylaxis prevents invasive aspergillosis. | aspergillus species are the major life threatening fungal pathogens in transplant patients. germination of inhaled fungal spores initiates infection, causes severe pneumonia, and has a mortality of >50%. this is leading to the consideration of pre-exposure prophylaxis to prevent infection. we made a very low mwt amphotericin b-polymethacrylic acid nanoparticle. it was not toxic to lung epithelial cells or monocyte-derived-macrophages in-vitro, or in an in-vivo transplant immuno-suppression mouse ... | 2015 | 25791815 |
| activity and safety of inhaled itraconazole nanosuspension in a model pulmonary aspergillus fumigatus infection in inoculated young quails. | pulmonary aspergillosis is frequently reported in parrots, falcons, and other birds held in captivity. inhalation is the main route of infection for aspergillus fumigatus, resulting in both acute and chronic disease conditions. itraconazole (itra) is an antifungal commonly used in birds, but its administration requires repeated oral dosing, and the safety margin is narrow. to investigate the efficacy of inhaled itra, six groups of ten young quails (coturnix japonica) were inoculated intratrachea ... | 2015 | 25790942 |
| age influence on mice lung tissue response to aspergillus fumigatus chronic exposure. | exposure to conidia of aspergillus fumigatus was described as a causative factor of a number of the respiratory system diseases, including asthma, chronic eosinophilic pneumonia, hypersensitivity pneumonitis and bronchopulmonary aspergillosis. the study investigates the effects of the repeated exposure to a. fumigatus in mice pulmonary compartment. our work tackles two, so far insufficiently addressed, important aspects of interaction between affected organism and a. fumigatus: 1) recurrent char ... | 2015 | 25780831 |
| in vitro and in vivo evaluation of small cationic abiotic lipopeptides as novel antifungal agents. | we investigated the antifungal potential of short lipopeptides against clinical fungal isolates with an objective to evaluate their clinical feasibility. all tested lipopeptides exhibit good antifungal activity with negligible difference between the mics against susceptible and drug-resistant clinical fungal isolates. the mtt assay results revealed the lower cytotoxicity of lipopeptides toward mammalian cells (nrk-52e). in particular, lp24 displayed highest potency against most of the tested fun ... | 2015 | 25777475 |
| the newly nonsporulated characterization of an aspergillus fumigatus isolate from an immunocompetent patient and its clinic indication. | aspergillus fumigatus (a. fumigatus) commonly produces abundant and heavily melanized infectious conidia, which are the primary agents that cause invasive aspergillosis (ia) in immunocompromised patients. we isolated a white nonsporulating a. fumigatus strain (a1j) from an immunocompetent patient. it was identified by histopathological examination and morphological observation, and subsequently confirmed by dna sequencing of internal transcribed spacer (its) regions and partial β-tubulin genes. ... | 2015 | 25776795 |
| mitochondrial dynamics in the pathogenic mold aspergillus fumigatus: therapeutic and evolutionary implications. | mitochondria within eukaryotic cells continuously fuse and divide. this phenomenon is called mitochondrial dynamics and crucial for mitochondrial function and integrity. we performed a comprehensive analysis of mitochondrial dynamics in the pathogenic mold aspergillus fumigatus. phenotypic characterization of respective mutants revealed the general essentiality of mitochondrial fusion for mitochondrial genome maintenance and the mold's viability. surprisingly, it turned out that the mitochondria ... | 2015 | 26272083 |
| evaluation of the double agar gel immunodiffusion test and of the enzyme-linked immunosorbent assay in the diagnosis and follow-up of patients with chronic pulmonary aspergillosis. | the diagnosis of chronic pulmonary aspergillosis (cpa) depends on the radiologic image and the identification of specific antibodies. the present study aimed to evaluate accuracy parameters of enzyme-linked immunosorbent assay (elisa) and of the determination of serum galactomannan level in the diagnosis of patients with cpa, comparing these results with the double agar gel immunodiffusion (did) test. in addition, the prevalence of cross-reactivity and the serological progression after treatment ... | 2015 | 26271000 |
| structure-functional characterization of cytochrome p450 sterol 14α-demethylase (cyp51b) from aspergillus fumigatus and molecular basis for the development of antifungal drugs. | aspergillus fumigatus is the opportunistic fungal pathogen that predominantly affects the immunocompromised population and causes 600,000 deaths/year. the cytochrome p450 51 (cyp51) inhibitor voriconazole is currently the drug of choice, yet the treatment efficiency remains low, calling for rational development of more efficient agents. a. fumigatus has two cyp51 genes, cyp51a and cyp51b, which share 59% amino acid sequence identity. cyp51b is expressed constitutively, whereas gene cyp51a is rep ... | 2015 | 26269599 |
| evidence for the involvement of cofilin in aspergillus fumigatus internalization into type ii alveolar epithelial cells. | the internalization of aspergillus fumigatus into alveolar epithelial cells (aecs) is tightly controlled by host cellular actin dynamics, which require close modulation of the adf (actin depolymerizing factor)/cofilin family. however, the role of cofilin in a. fumigatus internalization into aecs remains unclear. | 2015 | 26268695 |
| production of short-chain fatty acids from the biodegradation of wheat straw lignin by aspergillus fumigatus. | the wheat straw lignin-rich fraction (wslig-rf) can be used as a raw material for the production of metabolites for industrial use if ligninolytic mitosporic fungi are used for its biodegradation into aromatics and short-chain fatty acids (scfas, i.e., scfa2-6). although methods for the production of scfa2-6 have been described previously, quantitative data of scfa2-6 production have not been reported. the objectives of this study were to investigate the biodegradation of different concentration ... | 2015 | 26263005 |
| aspergillus in endodontic infection near the maxillary sinus. | diseases of the maxillary sinus have been associated with dental roots near the maxillary sinus that have undergone endodontic treatment. | 2015 | 26254203 |
| inhibition of aspergillus fumigatus and its biofilm by pseudomonas aeruginosa is dependent on the source, phenotype and growth conditions of the bacterium. | aspergillus fumigatus (af) and pseudomonas aeruginosa (pa) are leading fungal and bacterial pathogens, respectively, in many clinical situations. relevant to this, their interface and co-existence has been studied. in some experiments in vitro, pa products have been defined that are inhibitory to af. in some clinical situations, both can be biofilm producers, and biofilm could alter their physiology and affect their interaction. that may be most relevant to airways in cystic fibrosis (cf), where ... | 2015 | 26252384 |
| draft genome sequence of the pathogenic filamentous fungus aspergillus udagawae strain ifm 46973t. | the incidence of aspergillosis by aspergillus infection has dramatically increased in recent years. aspergillus udagawae, a species related to aspergillus fumigatus, is known as an emerging pathogen of aspergillosis. here, we present the draft genome sequence of a. udagawae strain ifm 46973(t). | 2015 | 26251487 |
| a case of late implantable cardiac device infection with aspergillus in an immunocompetent host. | with the increasing use of cardiac implantable electronic devices (cied), there has been an associated increase in rate of complications. infection accounts for about 1% of these, of which only a handful were reported secondary to aspergillus fumigatus. all of these were seen in chronically-ill patients with several co-morbid conditions within a few years of implantation. none have been reported in an otherwise immunocompetent patient at 7 years after cied implantation. | 2015 | 26250569 |
| allergic bronchopulmonary aspergillosis in an adult with kartagener syndrome. | allergic bronchopulmonary aspergillosis (abpa) is a pulmonary disorder resulting from immune responses directed against inhaled aspergillus fumigatus antigens. it manifests with poorly controlled asthma, fleeting pulmonary opacities and structural lung damage in the form of bronchiectasis. initially defined in individuals suffering from bronchial asthma and cystic fibrosis, it has also been described in patients with other structural lung disorders such as chronic obstructive pulmonary disease, ... | 2015 | 26250371 |
| drug-drug interaction between isavuconazole and tacrolimus: a case report indicating the need for tacrolimus drug-level monitoring. | despite the known significant drug-drug interaction between isavuconazole and tacrolimus, there are no recommendations on dose adjustment when these drugs are given concomitantly. we report on a patient with a mediastinal aspergillus fumigatus infection resistant to posaconazole and describe how she was successfully managed with tacrolimus therapeutic drug-level monitoring. | 2015 | 26248976 |
| calcium oxalate in the sputum may aid in the diagnosis of pulmonary aspergillosis: a report of two cases. | we present two cases of pulmonary aspergillosis in which calcium oxalate crystals in the sputum proved to be a useful diagnostic clue. in case 1, aspergillus hyphae was not identified; however, calcium oxalate crystals were present, and chronic necrotizing pulmonary aspergillosis was diagnosed. in case 2, calcium oxalate was detected and aspergillus fumigatus was identified later. thus, the presence of calcium oxalate in the sputum may be an important indicator for an a. fumigatus infection. | 2015 | 25834787 |
| fungi in cystic fibrosis and non-cystic fibrosis bronchiectasis. | bronchiectasis is a pathologic bronchial dilatation with loss of function that can result from multiple inflammatory and infectious injuries to the conducting airways of the lung. molds, particularly the filamentous fungus aspergillus fumigatus, have been implicated as a common cause of both cystic fibrosis (cf) and non-cf bronchiectasis, the latter primarily in patients with severe asthma. the pathogenesis of mold-associated bronchiectasis is usually due to atopic sensitization to mold allergen ... | 2015 | 25826588 |
| exposures and health outcomes in relation to bioaerosol emissions from composting facilities: a systematic review of occupational and community studies. | the number of composting sites in europe is rapidly increasing, due to efforts to reduce the fraction of waste destined for landfill, but evidence on possible health impacts is limited. this article systematically reviews studies related to bioaerosol exposures within and near composting facilities and associated health effects in both community and occupational health settings. six electronic databases and bibliographies from january 1960 to july 2014 were searched for studies reporting on heal ... | 2015 | 25825807 |
| azole drug import into the pathogenic fungus aspergillus fumigatus. | the fungal pathogen aspergillus fumigatus causes serious illness and often death when it invades tissues, especially in immunocompromised individuals. the azole class of drugs is the most commonly prescribed treatment for many fungal infections and acts on the ergosterol biosynthesis pathway. one common mechanism of acquired azole drug resistance in fungi is the prevention of drug accumulation to toxic levels in the cell. while drug efflux is a well-known resistance strategy, reduced azole impor ... | 2015 | 25824209 |
| cspa influences biofilm formation and drug resistance in pathogenic fungus aspergillus fumigatus. | the microbial cell wall plays a crucial role in biofilm formation and drug resistance. cspa encodes a repeat-rich glycophosphatidylinositol-anchored cell wall protein in the pathogenic fungus aspergillus fumigatus. to determine whether cspa has a significant impact on biofilm development and sensitivity to antifungal drugs in a. fumigatus, a δcspa mutant was constructed by targeted gene disruption, and we then reconstituted the mutant to wild type by homologous recombination of a functional cspa ... | 2015 | 25821832 |
| identification and mutational analyses of phosphorylation sites of the calcineurin-binding protein cbpa and the identification of domains required for calcineurin binding in aspergillus fumigatus. | calcineurin is a key protein phosphatase required for hyphal growth and virulence in aspergillus fumigatus, making it an attractive antifungal target. however, currently available calcineurin inhibitors, fk506 and cyclosporine a, are immunosuppressive, limiting usage in the treatment of patients with invasive aspergillosis. therefore, the identification of endogenous inhibitors of calcineurin belonging to the calcipressin family is an important parallel strategy. we previously identified the gen ... | 2015 | 25821446 |
| antibodies generated against streptococci protect in a mouse model of disseminated aspergillosis. | invasive aspergillosis (ia) resulting from infection by aspergillus fumigatus is a leading cause of death in immunosuppressed populations. there are limited therapeutic options for this disease and currently no vaccine. there is evidence that some anti-a. fumigatus mabs can provide protection against ia. however, vaccine development has been impeded by a paucity of immunological targets on this organism demonstrated to provide protective responses. sialylated oligosaccharide epitopes found on a ... | 2015 | 25821219 |
| aetiology of bronchiectasis in guangzhou, southern china. | aetiologies of bronchiectasis in mainland china and their comparisons with those in western countries are unknown. we aimed to investigate bronchiectasis aetiologies in guangzhou, southern china, and to determine ethnic or geographic differences with reports from western countries. | 2015 | 25819403 |
| preparative separation and purification of fumigaclavine c from fermented mycelia of aspergillus fumigatus cy018 by macroporous adsorption resin. | in this work, the separation and purification of fumigaclavine c (fc), an ergot alkaloid with strong anti-inflammatory activity from fermented mycelia of aspergillus fumigatus was systematically evaluated. among the eight tested resins, the non-polar resin d101 displayed the best adsorption and desorption based on of static adsorption and desorption tests. adsorption isotherms were constructed on d101 resin and fitted well to the freundlich model. dynamic adsorption and desorption tests on a col ... | 2015 | 25817261 |
| in vitro activity of calcium sulfate and hydroxyapatite antifungal disks loaded with amphotericin b or voriconazole in consideration for adjunctive osteomyelitis management. | regarding antibiotic-loaded cements, there is an abundant amount of literature regarding the antibacterial in vitro inhibitory and clinical applications for the treatment of osteomyelitis. the opposite can be said about literature regarding in vitro antifungal-loaded cement drug delivery for the treatment of fungal osteomyelitis. | 2015 | 25815648 |
| biomarker-based classification of bacterial and fungal whole-blood infections in a genome-wide expression study. | sepsis is a clinical syndrome that can be caused by bacteria or fungi. early knowledge on the nature of the causative agent is a prerequisite for targeted anti-microbial therapy. besides currently used detection methods like blood culture and pcr-based assays, the analysis of the transcriptional response of the host to infecting organisms holds great promise. in this study, we aim to examine the transcriptional footprint of infections caused by the bacterial pathogens staphylococcus aureus and e ... | 2015 | 25814982 |
| significant structural change in both o- and n-linked carbohydrate moieties of the antigenic galactomannan from aspergillus fumigatus grown under different culture conditions. | invasive aspergillosis is an important cause of morbidity and mortality in immunocompromised patients. diagnosis of this infection frequently employs detection of the circulating galactomannan in the patient serum using enzyme immunoassay (eia), a highly sensitive and specific system. although there are many structural studies of the galactomannan of aspergillus fumigatus, some inconsistencies are present in these results. in this study, to clarify the relationship between the growth conditions ... | 2015 | 25187160 |
| concomitant occurrence of itraconazole-resistant and -susceptible strains of aspergillus fumigatus in routine cultures. | resistance to triazoles in aspergillus fumigatus has emerged in several countries and is usually mediated by mutations in the cyp51a gene. we determined the presence of both itraconazole-susceptible and -resistant a. fumigatus in routine cultures isolated from environmental and clinical samples. | 2015 | 25326091 |
| comment on: susceptibility breakpoints and target values for therapeutic drug monitoring of voriconazole and aspergillus fumigatus in an in vitro pharmacokinetic/pharmacodynamic model. | 2015 | 25151206 | |
| effective treatment of invasive aspergillus fumigatus infection using combinations of topical and systemic antifungals in a severely burned patient. | the authors describe an invasive aspergillus fumigatus deep-burn wound infection in a severely burned patient that was successfully treated with a combination of topical terbinafine and systemic voriconazole antifungal therapy. to our knowledge, this is the first case report describing the effective control of an invasive deep-burn wound infection using this combination. | 2015 | 25127025 |
| chemical compositions and biological activities of the essential oils of beilschmiedia madang blume (lauraceae). | the present study aimed to examine the chemical compositions of the essential oils of beilschmiedia madang and their antioxidant, antibacterial, antifungal, anticholinesterase and anti-tyrosinase activities. the major constituents of the essential oils of leaf and bark of b. madang were δ-cadinene (17.0 and 20.5 %), β-caryophyllene (10.3 and 6.7 %), α-cubebene (11.3 and 15.6 %), and α-cadinol (5.8 and 10.6 %). the essential oils were screened for their antioxidant activities using 1,1-diphenyl-2 ... | 2015 | 25098422 |
| pattern recognition pathways leading to a th2 cytokine bias in allergic bronchopulmonary aspergillosis patients. | allergic bronchopulmonary aspergillosis (abpa) is characterised by an exaggerated th2 response to aspergillus fumigatus, but the immunological pathways responsible for this effect are unknown. | 2015 | 24912986 |
| isolation, screening, and identification of potential cellulolytic and xylanolytic producers for biodegradation of untreated oil palm trunk and its application in saccharification of lemongrass leaves. | this study presents the isolation and screening of fungi with excellent ability to degrade untreated oil palm trunk (opt) in a solid-state fermentation system (ssf). qualitative assay of cellulases and xylanase indicates notable secretion of both enzymes by 12 fungal strains from a laboratory collection and 5 strains isolated from a contaminated wooden board. high production of these enzymes was subsequently quantified in opt in ssf. aspergillus fumigates sk1 isolated from cow dung gives the hig ... | 2015 | 24960316 |
| role of inhaled amphotericin in allergic bronchopulmonary aspergillosis. | allergic bronchopulmonary aspergillosis (abpa) is an immunological pulmonary disorder caused by immune reactions mounted against the ubiquitous fungus aspergillus fumigatus. the disease clinically manifests with poorly controlled asthma, hemoptysis, systemic manifestations like fever, anorexia and weight loss, fleeting pulmonary opacities and bronchiectasis. the natural course of the disease is characterized by repeated episodes of exacerbations. almost 30-40% of the patients require prolonged t ... | 2015 | 24625938 |
| serpinb3/b4 contributes to early inflammation and barrier dysfunction in an experimental murine model of atopic dermatitis. | serine proteases are critical for epidermal barrier homeostasis, and their aberrant expression and/or activity is associated with chronic skin diseases. elevated levels of the serine protease inhibitors serpinb3 and serpinb4 are seen in patients with atopic dermatitis and psoriasis. however, their mechanistic role in the skin is unknown. to evaluate the contribution of serpinb3a (mouse homolog of serpinb3 and serpinb4) in atopic dermatitis, we examined the effect of topical aspergillus fumigatus ... | 2015 | 25111616 |
| elevated levels of the neutrophil chemoattractant pro-platelet basic protein in macrophages from individuals with chronic and allergic aspergillosis. | aspergillus fumigatus causes chronic cavitary pulmonary aspergillosis (ccpa) and allergic bronchopulmonary aspergillosis (abpa) in overtly immunocompetent and atopic individuals, respectively. disease mechanisms are poorly understood but may be related to increased neutrophil presence and activation. pro-platelet basic protein (ppbp) is a potent neutrophil chemoattractant and activator whose expression is repressed by interleukin 10 (il-10). | 2015 | 25193981 |
| [allergic bronchopulmonary aspergillosis]. | allergic bronchopulmonary aspergillosis is a slowly progressive disease, caused by the fungus aspergillus fumigatus hypersensitivity when it is found in the airway. it usually affects asthmatics and patients with cystic fibrosis. we report the case of a 20-year-old male patient, student, farmer and rancher with chronic respiratory disease. the diagnosis of allergic bronchopulmonary aspergillosis was made on the basis of the clinical symptoms and complementary studies. | 2015 | 24915624 |
| aspergillus endocarditis in a paediatric patient after a cardiac surgery, associated with septic pulmonary embolism and pulmonary hypertension. | we report a rare case of pulmonary prosthetic valve endocarditis due to aspergillus fumigatus, associated with septic pulmonary embolism and secondary pulmonary hypertension, in a 4-year-old boy with surgically corrected tetralogy of fallot. the diagnosis and treatment of aspergillus endocarditis remains highly challenging. the best therapeutic option for chronic thromboembolic pulmonary hypertension due to an infectious thromboembolic event is highly debatable and the results are poor. | 2015 | 24702799 |
| (1)h, (13)c and (15)n resonance assignments of the roda hydrophobin from the opportunistic pathogen aspergillus fumigatus. | hydrophobins are fungal proteins characterised by their amphipathic properties and an idiosyncratic pattern of eight cysteine residues involved in four disulphide bridges. the soluble form of these proteins spontaneously self-assembles at hydrophobic/hydrophilic interfaces to form an amphipathic monolayer. the roda hydrophobin of the opportunistic pathogen aspergillus fumigatus forms an amyloid layer with a rodlet morphology that covers the surface of fungal spores. this rodlet layer bestows hyd ... | 2015 | 24659460 |
| expression of the protein serum amyloid a in response to aspergillus fumigatus in murine models of allergic airway inflammation. | serum amyloid a (saa) is an acute phase protein that is elevated in blood during inflammation. the role of this protein in allergic diseases of airways remains unclear. | 2015 | 23603100 |
| in vitro antifungal properties of pistacia atlantica and olive extracts on different fungal species. | pistacia atlantica, which belongs to the anacardiaceae family, grows in the zagrossian region of iran. the aim of this study was to evaluate the antifungal properties of pistacia atlantica and olive leaf extracts against different fungal species. | 2015 | 28681004 |
| chemotypic and genotypic diversity in the ergot alkaloid pathway of aspergillus fumigatus. | aspergillus fumigatus is an opportunistic human pathogen that synthesizes a group of mycotoxins via a branch of the ergot alkaloid pathway. this fungus is globally distributed, and genetic data indicate that isolates recombine freely over that range; however, previous work on ergot alkaloids has focused on a limited number of isolates. we hypothesized that a. fumigatus harbors variation in the chemotype of ergot alkaloids and genotype of the ergot alkaloid gene cluster. analysis of 13 isolates b ... | 2015 | 22453123 |
| antifungal activity of terrestrial streptomyces rochei strain hf391 against clinical azole -resistant aspergillus fumigatus. | actinomycetes have been discovered as source of antifungal compounds that are currently in clinical use. invasive aspergillosis (ia) due to aspergillus fumigatus has been identified as individual drug-resistant aspergillus spp. to be an emerging pathogen opportunities a global scale. this paper described the antifungal activity of one terrestrial actinomycete against the clinically isolated azole-resistant a. fumigatus. | 2015 | 28680984 |
| accumulation of ergot alkaloids during conidiophore development in aspergillus fumigatus. | production of ergot alkaloids in the opportunistic fungal pathogen aspergillus fumigatus is restricted to conidiating cultures. these cultures typically accumulate several pathway intermediates at concentrations comparable to that of the pathway end product. we investigated the contribution of different cell types that constitute the multicellular conidiophore of a. fumigatus to the production of ergot alkaloid pathway intermediates versus the pathway end product, fumigaclavine c. a relatively m ... | 2014 | 23925951 |
| myocardial abscess, a rare form of cardiac aspergillosis. | 2014 | 23791602 | |
| environmental fungicides and triazole resistance in aspergillus. | fungal diseases are problematic in both human health and agriculture. treatment options are limited and resistance may emerge. the relatively recent recognition of triazole resistance in aspergillus fumigatus has prompted questioning of the origin of resistance. while multiple mechanisms are described in clinical isolates from triazole-treated patients, some de novo resistance is also recognised, especially attributable to tr34 /l98h. such strains probably arose in the environment, and, indeed, ... | 2014 | 23616354 |
| thermodynamics and kinetic properties of halostable endoglucanase from aspergillus fumigatus abk9. | an endoglucanase from aspergillus fumigatus abk9 was purified from the culture extract of solid-state fermentation and its some characteristics were evaluated. the molecular weight of the purified enzyme (56.3 kda) was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, zymogram analysis and confirmed by maldi-tof mass spectrometry. the enzyme was active optimally at 50 °c, ph 5.0 and stable over a broad range of ph (4.0-7.0) and nacl concentration of 0-3.0 m. the pka1 and p ... | 2014 | 23832828 |
| uncertain areas in the diagnosis of allergic bronchopulmonary aspergillosis in patients with asthma. | the prevalence of allergic bronchopulmonary aspergillosis (abpa) in patients with bronchial asthma remains unknown. we evaluated the roles of various laboratory tests in the diagnosis of abpa, including, skin prick test (spt) for aspergillus fumigatus (af), and serum af specific ige and igg antibody measurement. | 2014 | 23827645 |
| in vitro evaluation of portuguese propolis and floral sources for antiprotozoal, antibacterial and antifungal activity. | propolis is a beehive product with a very complex chemical composition, used since ancient times in several therapeutic treatments. as a contribution to the improvement of drugs against several tropical diseases caused by protozoa, we screened portuguese propolis and its potential floral sources populus x canadensis and cistus ladanifer against plasmodium falciparum, leishmania infantum, trypanosoma brucei and trypanosoma cruzi. the toxicity against mrc-5 fibroblast cells was evaluated to assess ... | 2014 | 23722631 |
| active site model of (r)-selective ω-transaminase and its application to the production of d-amino acids. | ω-transaminase (ω-ta) is one of the important biocatalytic toolkits owing to its unique enzyme property which enables the transfer of an amino group between primary amines and carbonyl compounds. in addition to preparation of chiral amines, ω-ta reactions have been exploited for the asymmetric synthesis of l-amino acids using (s)-selective ω-tas. however, despite the availability of (r)-selective ω-tas, catalytic utility of the ω-tas has not been explored for the production of d-amino acids. her ... | 2014 | 23576035 |
| stability of airborne microbes in the louvre museum over time. | the microbial content of air has as yet been little described, despite its public health implications, and there remains a lack of environmental microbial data on airborne microflora in enclosed spaces. in this context, the aim of this study was to characterize the diversity and dynamics of airborne microorganisms in the louvre museum using high-throughput molecular tools and to underline the microbial signature of indoor air in this human-occupied environment. this microbial community was monit ... | 2014 | 23710880 |
| modified culture method detects a high diversity of fungal species in cystic fibrosis patients. | cystic fibrosis (cf) is one of the most common genetic lung diseases worldwide. the production of sticky viscous mucus leads to enhanced bacterial colonization and infection, but yeasts and filamentous fungi are also found abundantly in the mucus of patients suffering from cf. the role of fungi in the airways of cf patients is still not understood completely. furthermore, recent investigations have shown that the spectrum of fungi isolated from the airways of cf patients depends strongly on the ... | 2014 | 23651180 |
| aspergillus fumigatus during stable state and exacerbations of copd. | bacteria are often isolated in stable chronic obstructive pulmonary disease (copd). whether fungi are also commonly present and associated with clinical and pathological features of disease is uncertain. we investigated the frequency of filamentous fungal culture and ige sensitisation to aspergillus fumigatus and the relationship to clinical outcomes in copd subjects. copd subjects were recruited to enter a 1-year observational study. assessments of lung function, allergen testing and sputum ana ... | 2014 | 23598955 |
| pharmacokinetic evaluation in mice of amorphous itraconazole-based dry powder formulations for inhalation with high bioavailability and extended lung retention. | three itraconazole (itz) dry powders for inhalation (dpi) were prepared by spray-drying a mannitol solution in which the itz was in suspension (f1) or was in solution without (f2) or with phospholipid (pl) (f3). these powders were endotracheally insufflated in vivo at a single dose of 0.5mg/kg for pharmacokinetic profile (lung and plasma concentration) determination in icr cd-1 mice. itz was crystalline in f1 and assumed to be amorphous in the f2 and f3 formulations. f2 and f3 formulations allow ... | 2014 | 23523546 |
| 68ga-triacetylfusarinine c and 68ga-ferrioxamine e for aspergillus infection imaging: uptake specificity in various microorganisms. | (68)ga-triacetylfusarinine c ((68)ga-tafc) and (68)ga-ferrioxamine e ((68)ga-foxe) showed excellent targeting properties in aspergillus fumigatus rat infection model. here, we report on the comparison of specificity towards different microorganisms and human lung cancer cells (h1299). | 2014 | 23818006 |
| volume dependency for culture of fungi from respiratory secretions and increased sensitivity of aspergillus quantitative pcr. | diagnosis of aspergillosis is often difficult. we compared fungal yields from respiratory specimens using the health protection agency standard culture method (bsop57), a higher volume undiluted culture method mycology reference centre manchester (mrcm) and aspergillus quantitative real time polymerase chain reaction (qpcr). sputum, bronchial aspirate and bronchoalveolar lavage (bal) samples (total 23) were collected from aspergillosis patients. one fraction of all samples was cultured using the ... | 2014 | 23786547 |
| discovering the infectome of human endothelial cells challenged with aspergillus fumigatus applying a mass spectrometry label-free approach. | blood vessel invasion is a key feature of invasive aspergillosis. this angioinvasion process contributes to tissue thrombosis, which can impair the access of leukocytes and antifungal drugs to the site of infection. it has been demonstrated that human umbilical vein endothelial cells (huvecs) are activated and assume a prothrombotic phenotype following contact with aspergillus fumigatus hyphae or germlings, a process that is independent of fungus viability. however, the molecular mechanisms by w ... | 2014 | 23886778 |
| activation of nf-κb and respiratory burst following aspergillus fumigatus stimulation of macrophages. | dectin-2, a c-type lectin receptor (clr), plays an essential role in mediating nuclear factor-kappa b (nf-κb) activation and anti-fungal immunity in response to candida albicans infection. however, the molecular mechanisms and function of dectin-2 signaling in response to infection by the pathogenic fungus aspergillus fumigatus have not been characterized. in order to characterize dectin-2 signaling in response to a. fumigatus infection, activation of dectin-2 was analyzed at both transcriptiona ... | 2014 | 23886693 |
| biofilm formation by aspergillus fumigatus. | aspergillus fumigatus is a well adapted, opportunistic fungus that causes a severe and commonly fatal disease, invasive pulmonary aspergillosis (ipa), in highly immunocompromised patients, aspergilloma in patients with lung cavities and allergic bronchopulmonary aspergillosis (abpa) in hypersensitive individuals. recent studies have suggested that biofilm formation by a. fumigatus may be one of the most important virulence factors in ipa and aspergilloma. several fungal constituents may contribu ... | 2014 | 23962172 |
| inhibition of conidiophore development in aspergillus fumigatus by an escherichia coli dh5α strain, a promising antifungal candidate against aspergillosis. | the opportunistic human pathogen aspergillus fumigatus produces a massive number of asexual spores (conidia) as the primary means of dispersal, survival, genome protection and infection of hosts. in this report, we investigated secretory and cytosolic proteins of non-pathogenic bacterial species (mostly belonging to human microbiome) for antifungal potential against a. fumigatus, a. flavus and a. niger. our preliminary results revealed that cytosolic proteins of e. coli dh5α were most active and ... | 2014 | 23978655 |
| acute chiasmal abscess resulting from perineural extension of invasive sino-orbital aspergillosis in an immunocompetent patient. | intracranially invasive sino-orbital aspergillosis is a rare entity seen predominantly in immunocompromised individuals. we report a unique case of an acute chiasmal abscess resulting from perineural extension of an indolent invasive sino-orbital aspergillosis in an immunocompetent patient. | 2014 | 23973515 |
| an anti-aspergillus protein from escherichia coli dh5α: putative inhibitor of siderophore biosynthesis in aspergillus fumigatus. | an antifungal protein designated as anti-aspergillus protein (aap), produced by escherichia coli dh5α, was purified and characterised. it exhibited a molecular weight of 60 kda on sodium dodecyl sulphate-polyacrylamide gel electrophoresis analysis and depicted 99% purity on ultra performance liquid chromatography. the purified protein manifested antimycotic potential against pathogenic isolates of aspergillus spp., depicting a minimum inhibitory concentration in the range 15.62-31.25 μg ml(-1) a ... | 2014 | 23968167 |
| different colonization patterns of aspergillus terreus in patients with cystic fibrosis. | aspergillus terreus is a common soil saprophyte. after aspergillus fumigatus and scedosporium apiospermum it ranks third amongst the filamentous fungi colonizing the airways of patients with cystic fibrosis. in this context, the clinical presentation of a. terreus infection mainly corresponds to allergic broncho-pulmonary aspergillosis. in the work presented here, we studied colonization patterns of a. terreus in cf patients by genotyping using nine short tandem repeat markers. a total of 115 cl ... | 2014 | 23927682 |
| synthesis and characterization of some novel antimicrobial thiosemicarbazone o-carboxymethyl chitosan derivatives. | three novel thiosemicarbazone o-carboxymethyl chitosan derivatives were obtained via a condensation reaction of thiosemicarbazide o-carboxymethyl chitosan with o-hydroxybenzaldehyde, p-methoxybenzaldehyde, and p- chlorobenzaldehyde respectively. their structures were characterized by elemental analysis, ftir, (13)c nmr and x-ray diffraction. the antimicrobial behaviors of the prepared derivatives against three types of bacteria staphylococcus aureus (s. aureus, rcmba 2004), bacillus subtilis (b. ... | 2014 | 24211430 |
| recurrent pulmonary aspergillosis and mycobacterial infection in an unsplenectomized patient with type 1 gaucher disease. | the clinical presentation of gaucher disease (gd), an inherited lysosomal storage disorder caused by the deficient activity of the lysosomal enzyme glucocerebrosidase, is highly variable, and three clinical types are distinguished based upon the presence of neurologic symptoms. thrombocytopenia, anemia, hepatosplenomegaly, and bone manifestations are the most typical signs of gd type 1 (gd1). | 2014 | 24195576 |
| the aspergillus genome database: multispecies curation and incorporation of rna-seq data to improve structural gene annotations. | the aspergillus genome database (aspgd; http://www.aspgd.org) is a freely available web-based resource that was designed for aspergillus researchers and is also a valuable source of information for the entire fungal research community. in addition to being a repository and central point of access to genome, transcriptome and polymorphism data, aspgd hosts a comprehensive comparative genomics toolbox that facilitates the exploration of precomputed orthologs among the 20 currently available asperg ... | 2014 | 24194595 |
| the fungal upr: a regulatory hub for virulence traits in the mold pathogen aspergillus fumigatus. | aspergillus fumigatus is an opportunistic pathogen that is responsible for a life-threatening fungal infection known as invasive aspergillosis. current therapies for the treatment of this disease continue to be associated with a poor outcome, so there is a need for more information about aspects of the fungus-host interaction that could offer novel targets for drug intervention. one attractive possibility is the unfolded protein response (upr), an intracellular signaling network that helps the f ... | 2014 | 24189125 |
| human natural killer cells acting as phagocytes against candida albicans and mounting an inflammatory response that modulates neutrophil antifungal activity. | natural killer (nk) cells are innate lymphocytes with potent cytotoxic activity. whereas activity of nk cells has been demonstrated against the fungal pathogens aspergillus fumigatus and cryptococcus neoformans, little was known about their interaction with candida albicans. | 2014 | 24163416 |
| role of carbonic anhydrases in pathogenic micro-organisms: a focus on aspergillus fumigatus. | aspergillus fumigatus is a ubiquitous saprophytic fungus responsible for organic material decomposition, and plays an important role in recycling environmental carbon and nitrogen. besides its important role in the environment, this fungus has been reported as one of the most important fungal pathogens in immunocompromised patients. due to changes in co2 concentration that some pathogens face during the infection process, studies have been undertaken to understand the pathogenic roles of carboni ... | 2014 | 24149624 |
| biosorption characteristics of aspergillus fumigatus for the decolorization of triphenylmethane dye acid violet 49. | this study focuses on the possible use of aspergillus fumigatus to remove acid violet 49 dye (av49) from aqueous solution. in batch biosorption experiments, the highest biosorption efficiency was achieved at ph 3.0, with biosorbent dosage of 3.0 gl(-1) within about 30 min at 40 °c. the langmuir and freundlich models were able to describe the biosorption equilibrium of av49 onto fungal biomass with maximum dye uptake capacity 136.98 mg g(-1). biosorption followed a pseudo-second-order kinetic mod ... | 2014 | 24136473 |
| deletion of the putative stretch-activated ion channel mid1 is hypervirulent in aspergillus fumigatus. | calcium ion is a universal and physiologically important molecule affecting almost every cellular function, while mid1 (mating induced death) is a stretch-activated ion channel localized in the plasma membrane, which can replenish the fungus with extracellular calcium. aspergillus fumigatus is one of the most important fungal pathogens but little is known about its calcium channels in the plasma membrane. in this study, a homolog of saccharomyces cerevisiae mid1 was identified in a. fumigatus an ... | 2014 | 24239700 |
| assessment of selection and resistance risk for demethylation inhibitor fungicides in aspergillus fumigatus in agriculture and medicine: a critical review. | an increasing number of publications have claimed that demethylation inhibitor (dmi) fungicides are confronted with resistance development in the fungus aspergillus fumigatus and that the origin of resistant isolates may also be outside the medical area. for resistance risk assessment and sourcing the origin of dmi resistance, the primary exposure events ofa. fumigatus with dmi treatments have been analysed case by case, resulting in the pathogen exposure risk (per). | 2014 | 24123539 |
| exopolysaccharides and antimicrobial biosurfactants produced by paenibacillus macerans tku029. | paenibacillus macerans tku029 can produce exopolysaccharides (epss; 3.46 g/l) and a biosurfactant (1.78 g/l) in a medium with 2 % (w/v) squid pen powder as the sole carbon/nitrogen source. the biosurfactant can reduce the surface tension of water from 72.30 to 35.34 mn/m at a concentration of 2.76 g/l and reach an emulsification index of 56 % after a 24-h reaction with machine oil. this biosurfactant is stable at 121 °c for 20 min, over a ph range from 3 to 11, and in <5 % salt solutions. it als ... | 2014 | 24122708 |
| genomic identification of potential targets unique to candida albicans for the discovery of antifungal agents. | despite of modern antifungal therapy, the mortality rates of invasive infection with human fungal pathogen candida albicans are up to 40%. studies suggest that drug resistance in the three most common species of human fungal pathogens viz., c. albicans, aspergillus fumigatus (causing mortality rate up to 90%) and cryptococcus neoformans (causing mortality rate up to 70%) is due to mutations in the target enzymes or high expression of drug transporter genes. drug resistance in human fungal pathog ... | 2014 | 24102473 |
| effect of physical and chemical properties of oil palm empty fruit bunch, decanter cake and sago pith residue on cellulases production by trichoderma asperellum upm1 and aspergillus fumigatus upm2. | the effect of cultivation condition of two locally isolated ascomycetes strains namely trichoderma asperellum upm1 and aspergillus fumigatus upm2 were compared in submerged and solid state fermentation. physical evaluation on water absorption index, solubility index and chemical properties of lignin, hemicellulose and cellulose content as well as the cellulose structure on crystallinity and amorphous region of treated oil palm empty fruit bunch (opefb) (resulted in partial removal of lignin), sa ... | 2014 | 24085387 |
| azole-resistant aspergillus fumigatus with the environmental tr46/y121f/t289a mutation in india. | 2014 | 24084639 | |
| signaling mechanism for aspergillus fumigatus tolerance in corneal fibroblasts induced by lps pretreatment. | tlrs, particularly tlr2 and tlr4, play primary roles in inflammatory responses triggered by aspergillus fumigatus and lead to the activation of signaling pathways that initiate host defense responses. we previously demonstrated that lps, a ligand of tlr4, can induce tolerance of a. fumigatus hyphae in telomerase-immortalized human stroma fibroblasts (thsfs). in the present study we investigated the role of tlr4, tlr2 and their downstream signaling pathways in this activity. the thsfs were pretre ... | 2014 | 24045340 |
| antifungal dimeric chalcone derivative kamalachalcone e from mallotus philippinensis. | from the red coloured extract (kamala) prepared through acetone extraction of the fresh whole uncrushed fruits of mallotus philippinensis, one new dimeric chalcone (1) along with three known compounds 1-(5,7-dihydroxy-2,2,6-trimethyl-2h-1-benzopyran-8-yl)-3-phenyl-2-propen-1-one (2), rottlerin (3) and 4'-hydroxyrottlerin (4) were isolated. the structure of compound 1 was elucidated by 1d and 2d nmr analyses that included hsqc, hmbc, cosy and roesy experiments along with the literature comparison ... | 2014 | 24099509 |
| transcriptional activation of heat shock protein 90 mediated via a proximal promoter region as trigger of caspofungin resistance in aspergillus fumigatus. | invasive aspergillosis is a deadly infection for which new antifungal therapies are needed. heat shock protein 90 (hsp90) is an essential chaperone in aspergillus fumigatus representing an attractive antifungal target. using a thiamine-repressible promoter (pthia), we showed that genetic repression of hsp90 significantly reduced virulence in a murine model of invasive aspergillosis. moreover, substituting the a. fumigatus hsp90 promoter with 2 artificial promoters (potef, pthia) and the candida ... | 2014 | 24096332 |
| oxidative burst and neutrophil elastase contribute to clearance of aspergillus fumigatus pneumonia in mice. | polymorphonuclear neutrophils (pmn) are important for the control of invasive aspergillosis (ia), a major threat to immunocompromised individuals. for clearance of aspergillus fumigatus infections, pmn employ their potent oxidative and non-oxidative mechanisms. to clarify the relative contribution of these mechanisms, we analyzed p47(phox-/-), gp91(phox-/-) and elastase (ela) deficient mice (elane) after intratracheal infection with a. fumigatus. infected p47(phox-/-) and gp91(phox-/-) mice died ... | 2014 | 24054721 |
| death causes and pathogens analysis of systemic lupus erythematosus during the past 26 years. | different causes of mortality have been described over different decades followed by description of pathogens identified from infective episodes that led to death. a retrospective review was performed in 3,831 hospitalized systemic lupus erythematosus (sle) patients in peking union medical college hospital from january 1986 to april 2012. the primary causes of death were identified, and the constituent ratio of specific death causes during different periods was compared. among 3,831 hospitalized ... | 2014 | 24046218 |
| discrimination of aspergillus lentulus from aspergillus fumigatus by raman spectroscopy and maldi-tof ms. | in 2005, a new sibling species of aspergillus fumigatus was discovered: aspergillus lentulus. both species can cause invasive fungal disease in immune-compromised patients. the species are morphologically very similar. current techniques for identification are pcr-based or morphology-based. these techniques are labour-intense and not sufficiently discriminatory. since a. lentulus is less susceptible to several antifungal agents, it is important to correctly identify the causative infectious agen ... | 2014 | 24030717 |
| comparison of methods for isolation of bacterial and fungal dna from human blood. | the study aimed at optimization of dna isolation from blood of representatives of four microbial groups causing sepsis, i.e., gram negative: escherichia coli, gram positive: staphylococcus aureus, yeast: candida albicans, and filamentous fungus: aspergillus fumigatus. additionally, the five commercial kits for microbial dna isolation from the blood were tested. the developed procedure of dna isolation consisted of three consecutive steps, i.e., mechanical disruption, chemical lysis, and thermal ... | 2014 | 24026449 |
| comparative evaluation of galactomannan optical density indices and culture results in bronchoscopic specimens obtained from neutropenic and non-neutropenic patients. | aspergillus infections are major causes of morbidity and mortality among immunocompromised patients. this study was designed to investigate the galactomannan assay optical density (od) indices relative to the culture results in bronchoscopic samples obtained from neutropenic and non-neutropenic patients. galactomannan od indices from 1427 samples from 2005 to 2012, which were sent from 839 patients and were composed of bronchial lavage (bl = 727) and bronchoalveolar lavage fluids (bal = 700), we ... | 2014 | 24010950 |